Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G24840 |
| fragaria_vesca | FvH4_2g36340 |
| malus_domestica | MD08G1121000.v1.1 MD15G1100000.v1.1 |
| prunus_persica | Prupe.1G454800_v2.0.a1 |
| pyrus_communis | pycom08g10110 pycom15g09200 |
| rosa_chinensis | RchiOBHm_Chr6g0305541 |
| rosa_laevigata | RLG00000010878 |
| rosa_multiflora | Rmu_co8330221.1_g000001 Rmu_sc0000841.1_g000004 |
| rosa_roxburghii | Rroxscaffold_7G00162600 |
| rosa_rugosa | Rorug06G0341500 |
| rosa_samantha | Rh6AG453500 Rh6BG448100 Rh6CG467300 Rh6DG453900 |
| rosa_wichuraiana | Rw6G039520 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 741 |
| AciI | CCGC | 2 cut(s) | 155, 189 |
| AclWI | GGATC | 5 cut(s) | 193, 332, 449, 462, 644 |
| AcoI | YGGCCR | 1 cut(s) | 352 |
| AfaI | GTAC | 1 cut(s) | 114 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 451 |
| AflIII | ACRYGT | 1 cut(s) | 739 |
| AgsI | TTSAA | 4 cut(s) | 252, 466, 569, 620 |
| AjnI | CCWGG | 2 cut(s) | 124, 349 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 581, 613 |
| AluBI | AGCT | 6 cut(s) | 289, 427, 623, 640, 664, 728 |
| AluI | AGCT | 6 cut(s) | 289, 427, 623, 640, 664, 728 |
| Alw21I | GWGCWC | 2 cut(s) | 79, 642 |
| Alw26I | GTCTC | 1 cut(s) | 260 |
| AlwI | GGATC | 5 cut(s) | 193, 332, 449, 462, 644 |
| Ama87I | CYCGRG | 1 cut(s) | 263 |
| AoxI | GGCC | 2 cut(s) | 43, 352 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 59 |
| AsuC2I | CCSGG | 1 cut(s) | 452 |
| AsuHPI | GGTGA | 2 cut(s) | 313, 352 |
| AsuII | TTCGAA | 1 cut(s) | 431 |
| AvaI | CYCGRG | 1 cut(s) | 263 |
| BalI | TGGCCA | 1 cut(s) | 354 |
| BamHI | GGATCC | 1 cut(s) | 454 |
| BanII | GRGCYC | 1 cut(s) | 642 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 47, 79 |
| Bbv12I | GWGCWC | 2 cut(s) | 79, 642 |
| BccI | CCATC | 3 cut(s) | 125, 486, 757 |
| BceAI | ACGGC | 1 cut(s) | 468 |
| BciT130I | CCWGG | 2 cut(s) | 126, 351 |
| BclI | TGATCA | 1 cut(s) | 175 |
| BcnI | CCSGG | 1 cut(s) | 452 |
| BcoDI | GTCTC | 1 cut(s) | 260 |
| BfaI | CTAG | 1 cut(s) | 530 |
| Bme1390I | CCNGG | 3 cut(s) | 126, 351, 452 |
| BmeT110I | CYCGRG | 1 cut(s) | 263 |
| BmiI | GGNNCC | 1 cut(s) | 456 |
| BmrFI | CCNGG | 3 cut(s) | 126, 351, 452 |
| Bpu14I | TTCGAA | 1 cut(s) | 431 |
| BpuMI | CCSGG | 1 cut(s) | 452 |
| BsaJI | CCNNGG | 2 cut(s) | 50, 124 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 451 |
| Bse1I | ACTGG | 6 cut(s) | 46, 110, 202, 513, 587, 653 |
| BseBI | CCWGG | 2 cut(s) | 126, 351 |
| BseDI | CCNNGG | 2 cut(s) | 50, 124 |
| BseGI | GGATG | 4 cut(s) | 517, 591, 607, 749 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 451 |
| BseMII | CTCAG | 2 cut(s) | 83, 384 |
| BseNI | ACTGG | 6 cut(s) | 46, 110, 202, 513, 587, 653 |
| Bsh1236I | CGCG | 1 cut(s) | 741 |
| BshFI | GGCC | 2 cut(s) | 45, 354 |
| BsiHKAI | GWGCWC | 2 cut(s) | 79, 642 |
| BsiHKCI | CYCGRG | 1 cut(s) | 263 |
| BsiSI | CCGG | 1 cut(s) | 452 |
| BslFI | GGGAC | 2 cut(s) | 246, 726 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 451 |
| BsmAI | GTCTC | 1 cut(s) | 260 |
| BsmBI | CGTCTC | 1 cut(s) | 260 |
| BsmFI | GGGAC | 2 cut(s) | 246, 726 |
| BsnI | GGCC | 2 cut(s) | 45, 354 |
| BsoBI | CYCGRG | 1 cut(s) | 263 |
| Bsp119I | TTCGAA | 1 cut(s) | 431 |
| Bsp1286I | GDGCHC | 2 cut(s) | 79, 642 |
| Bsp143I | GATC | 6 cut(s) | 175, 198, 276, 324, 454, 649 |
| BspACI | CCGC | 2 cut(s) | 155, 189 |
| BspANI | GGCC | 2 cut(s) | 45, 354 |
| BspCNI | CTCAG | 2 cut(s) | 84, 383 |
| BspFNI | CGCG | 1 cut(s) | 741 |
| BspLI | GGNNCC | 1 cut(s) | 456 |
| BspPI | GGATC | 5 cut(s) | 193, 332, 449, 462, 644 |
| BspQI | GCTCTTC | 1 cut(s) | 630 |
| BspT104I | TTCGAA | 1 cut(s) | 431 |
| BsrI | ACTGG | 6 cut(s) | 46, 110, 202, 513, 587, 653 |
| BssECI | CCNNGG | 2 cut(s) | 50, 124 |
| BssMI | GATC | 6 cut(s) | 175, 198, 276, 324, 454, 649 |
| Bst2UI | CCWGG | 2 cut(s) | 126, 351 |
| Bst6I | CTCTTC | 2 cut(s) | 150, 630 |
| BstBI | TTCGAA | 1 cut(s) | 431 |
| BstC8I | GCNNGC | 2 cut(s) | 47, 595 |
| BstDEI | CTNAG | 3 cut(s) | 92, 290, 370 |
| BstF5I | GGATG | 4 cut(s) | 517, 591, 607, 749 |
| BstFNI | CGCG | 1 cut(s) | 741 |
| BstKTI | GATC | 6 cut(s) | 178, 201, 279, 327, 457, 652 |
| BstMAI | GTCTC | 1 cut(s) | 260 |
| BstMBI | GATC | 6 cut(s) | 175, 198, 276, 324, 454, 649 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 55 |
| BstNI | CCWGG | 2 cut(s) | 126, 351 |
| BstSCI | CCNGG | 3 cut(s) | 124, 349, 450 |
| BstUI | CGCG | 1 cut(s) | 741 |
| BstX2I | RGATCY | 3 cut(s) | 198, 324, 454 |
| BstYI | RGATCY | 3 cut(s) | 198, 324, 454 |
| BsuRI | GGCC | 2 cut(s) | 45, 354 |
| BtgZI | GCGATG | 1 cut(s) | 464 |
| BtsCI | GGATG | 4 cut(s) | 517, 591, 607, 749 |
| BtsIMutI | CAGTG | 1 cut(s) | 378 |
| Cac8I | GCNNGC | 2 cut(s) | 47, 595 |
| CseI | GACGC | 2 cut(s) | 277, 747 |
| Csp6I | GTAC | 1 cut(s) | 113 |
| CviAII | CATG | 2 cut(s) | 131, 391 |
| CviQI | GTAC | 1 cut(s) | 113 |
| DdeI | CTNAG | 3 cut(s) | 92, 290, 370 |
| DpnI | GATC | 6 cut(s) | 177, 200, 278, 326, 456, 651 |
| DpnII | GATC | 6 cut(s) | 175, 198, 276, 324, 454, 649 |
| EaeI | YGGCCR | 1 cut(s) | 352 |
| Eam1104I | CTCTTC | 2 cut(s) | 150, 630 |
| EarI | CTCTTC | 2 cut(s) | 150, 630 |
| Ecl136II | GAGCTC | 1 cut(s) | 640 |
| Eco24I | GRGCYC | 1 cut(s) | 642 |
| Eco53kI | GAGCTC | 1 cut(s) | 640 |
| Eco88I | CYCGRG | 1 cut(s) | 263 |
| EcoICRI | GAGCTC | 1 cut(s) | 640 |
| EcoRII | CCWGG | 2 cut(s) | 124, 349 |
| EcoT38I | GRGCYC | 1 cut(s) | 642 |
| Esp3I | CGTCTC | 1 cut(s) | 260 |
| FaeI | CATG | 2 cut(s) | 134, 394 |
| FaiI | YATR | 9 cut(s) | 132, 312, 392, 519, 525, 548, 550, 614, 757 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 411, 443, 581, 613 |
| FaqI | GGGAC | 2 cut(s) | 246, 726 |
| FatI | CATG | 2 cut(s) | 130, 390 |
| FauI | CCCGC | 1 cut(s) | 162 |
| FbaI | TGATCA | 1 cut(s) | 175 |
| FokI | GGATG | 4 cut(s) | 524, 594, 598, 736 |
| FriOI | GRGCYC | 1 cut(s) | 642 |
| FspBI | CTAG | 1 cut(s) | 530 |
| HaeIII | GGCC | 2 cut(s) | 45, 354 |
| HapII | CCGG | 1 cut(s) | 452 |
| HgaI | GACGC | 2 cut(s) | 277, 747 |
| Hin1II | CATG | 2 cut(s) | 134, 394 |
| HincII | GTYRAC | 2 cut(s) | 39, 700 |
| HindII | GTYRAC | 2 cut(s) | 39, 700 |
| HinfI | GANTC | 2 cut(s) | 98, 604 |
| HpaII | CCGG | 1 cut(s) | 452 |
| HphI | GGTGA | 2 cut(s) | 313, 352 |
| Hpy166II | GTNNAC | 4 cut(s) | 39, 184, 700, 733 |
| Hpy188I | TCNGA | 8 cut(s) | 7, 54, 93, 175, 281, 381, 405, 725 |
| Hpy188III | TCNNGA | 1 cut(s) | 530 |
| Hpy8I | GTNNAC | 4 cut(s) | 39, 184, 700, 733 |
| HpyAV | CCTTC | 3 cut(s) | 583, 680, 688 |
| HpyCH4V | TGCA | 3 cut(s) | 209, 677, 746 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 55 |
| HpyF3I | CTNAG | 3 cut(s) | 92, 290, 370 |
| Hsp92II | CATG | 2 cut(s) | 134, 394 |
| Ksp22I | TGATCA | 1 cut(s) | 175 |
| Kzo9I | GATC | 6 cut(s) | 175, 198, 276, 324, 454, 649 |
| LguI | GCTCTTC | 1 cut(s) | 630 |
| LmnI | GCTCC | 1 cut(s) | 286 |
| MaeI | CTAG | 1 cut(s) | 530 |
| MaeIII | GTNAC | 4 cut(s) | 95, 301, 340, 655 |
| MalI | GATC | 6 cut(s) | 177, 200, 278, 326, 456, 651 |
| MboI | GATC | 6 cut(s) | 175, 198, 276, 324, 454, 649 |
| MboII | GAAGA | 4 cut(s) | 137, 445, 581, 647 |
| MflI | RGATCY | 3 cut(s) | 198, 324, 454 |
| MhlI | GDGCHC | 2 cut(s) | 79, 642 |
| MlsI | TGGCCA | 1 cut(s) | 354 |
| MluCI | AATT | 3 cut(s) | 385, 406, 555 |
| MluI | ACGCGT | 1 cut(s) | 739 |
| MluNI | TGGCCA | 1 cut(s) | 354 |
| MlyI | GAGTC | 1 cut(s) | 92 |
| MnlI | CCTC | 6 cut(s) | 60, 99, 178, 468, 526, 676 |
| Mox20I | TGGCCA | 1 cut(s) | 354 |
| MroXI | GAANNNNTTC | 1 cut(s) | 59 |
| MscI | TGGCCA | 1 cut(s) | 354 |
| MseI | TTAA | 1 cut(s) | 335 |
| MslI | CAYNNNNRTG | 1 cut(s) | 754 |
| Msp20I | TGGCCA | 1 cut(s) | 354 |
| MspA1I | CMGCKG | 1 cut(s) | 189 |
| MspI | CCGG | 1 cut(s) | 452 |
| MspR9I | CCNGG | 3 cut(s) | 126, 351, 452 |
| MvaI | CCWGG | 2 cut(s) | 126, 351 |
| MvnI | CGCG | 1 cut(s) | 741 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 55 |
| NciI | CCSGG | 1 cut(s) | 452 |
| NdeII | GATC | 6 cut(s) | 175, 198, 276, 324, 454, 649 |
| NlaIII | CATG | 2 cut(s) | 134, 394 |
| NlaIV | GGNNCC | 1 cut(s) | 456 |
| NmuCI | GTSAC | 3 cut(s) | 95, 301, 340 |
| NspV | TTCGAA | 1 cut(s) | 431 |
| PciSI | GCTCTTC | 1 cut(s) | 630 |
| PdmI | GAANNNNTTC | 1 cut(s) | 59 |
| PfeI | GAWTC | 1 cut(s) | 604 |
| PfoI | TCCNGGA | 1 cut(s) | 450 |
| PleI | GAGTC | 1 cut(s) | 92 |
| PpsI | GAGTC | 1 cut(s) | 92 |
| Psp124BI | GAGCTC | 1 cut(s) | 642 |
| Psp6I | CCWGG | 2 cut(s) | 124, 349 |
| PspGI | CCWGG | 2 cut(s) | 124, 349 |
| PspN4I | GGNNCC | 1 cut(s) | 456 |
| PsuI | RGATCY | 3 cut(s) | 198, 324, 454 |
| RsaI | GTAC | 1 cut(s) | 114 |
| RsaNI | GTAC | 1 cut(s) | 113 |
| RseI | CAYNNNNRTG | 1 cut(s) | 754 |
| SacI | GAGCTC | 1 cut(s) | 642 |
| SapI | GCTCTTC | 1 cut(s) | 630 |
| SaqAI | TTAA | 1 cut(s) | 335 |
| Sau3AI | GATC | 6 cut(s) | 175, 198, 276, 324, 454, 649 |
| SchI | GAGTC | 1 cut(s) | 92 |
| ScrFI | CCNGG | 3 cut(s) | 126, 351, 452 |
| SduI | GDGCHC | 2 cut(s) | 79, 642 |
| SfuI | TTCGAA | 1 cut(s) | 431 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 754 |
| Sse9I | AATT | 3 cut(s) | 385, 406, 555 |
| SsiI | CCGC | 2 cut(s) | 155, 189 |
| SspMI | CTAG | 1 cut(s) | 530 |
| SstI | GAGCTC | 1 cut(s) | 642 |
| StyD4I | CCNGG | 3 cut(s) | 124, 349, 450 |
| TaqI | TCGA | 1 cut(s) | 431 |
| TasI | AATT | 3 cut(s) | 385, 406, 555 |
| TfiI | GAWTC | 1 cut(s) | 604 |
| Tru1I | TTAA | 1 cut(s) | 335 |
| Tru9I | TTAA | 1 cut(s) | 335 |
| TscAI | CASTG | 1 cut(s) | 378 |
| TseFI | GTSAC | 3 cut(s) | 95, 301, 340 |
| Tsp45I | GTSAC | 3 cut(s) | 95, 301, 340 |
| TspDTI | ATGAA | 4 cut(s) | 137, 407, 602, 648 |
| TspRI | CASTG | 1 cut(s) | 378 |
| XbaI | TCTAGA | 1 cut(s) | 529 |
| XmnI | GAANNNNTTC | 1 cut(s) | 59 |
| XspI | CTAG | 1 cut(s) | 530 |