Rh6BG448100

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0012895
Physical Location & Seq
Reverse (-)
66318951 .. 66320224
1274 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG448100.1

Sequence Viewer

Length: 759 bp
ATGTTGGAAAACAAAGTAAACTCAACTCATAGCAAGTCCACTGGTCTGCCTCGGAAGCGTTTCTACAGAGCAAGAGCACACAGTAACCCTCTGAGTGACTCCCACTTTCCAGTACCTGTCGCCCCCAGTCATGTGGATTATTCTCTTCATTACCCGCAGCTCTTCCCCTCATCTGATGAAGCTGGTAGTTGCAAAAAGATCCAGTTTGCAGATATTGGCTGTGGTTTTGGGGGGCTTCTGATAAGCCTTTCAACTCTTTTCCCTAGCACTCTGATGATTGGAATGGAGCTTAGGGATAAGGTGACAGAGTATGTCAAGGAAAGGATTTTGGCTTTAAGGGTGACAAATCCGGGTCAATACCAGAATATCTCAGTGGTTCGCACTAATTCCATGAAGTACATCCCAAATTACTTTGAGAAAGCACAGCTTTCAAAGATGTTTTTCCTGTTCCCGGATCCTCACTTCAAAGAGAAAAATCACCGTCGTAGGGTGATCAGTCCATTCTTGCTGGATGAGTATGCTTATGCTCTAGAAGTTGGTGGCATTATATACACAATAACAGATGTTGAAGAACTTGGAGACTGGATGAAGGCTTGTTTAGAGAATCACCCTATGTTTGAAGCTCTCACAAGTGAAGAGGTCGAAGCAGATCCAGTTACGAAGCTCTTGAGTTCTGCAACTGAGGAAGGACAGAAGGTGGACAGAAACGGGGGACAGACTTTCCGAGCAATTTACAGACGCATTGCTCCATCCCTATGA

Protein Analysis

252

Amino Acids

28.6

Weight (kDa)

7.74

Isoelectric Point (pI)

46.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_4 PF02390 67 - 242 1.4e-49 Putative methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012895)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24840
fragaria_vesca FvH4_2g36340
malus_domestica MD08G1121000.v1.1 MD15G1100000.v1.1
prunus_persica Prupe.1G454800_v2.0.a1
pyrus_communis pycom08g10110 pycom15g09200
rosa_chinensis RchiOBHm_Chr6g0305541
rosa_laevigata RLG00000010878
rosa_multiflora Rmu_co8330221.1_g000001 Rmu_sc0000841.1_g000004
rosa_roxburghii Rroxscaffold_7G00162600
rosa_rugosa Rorug06G0341500
rosa_samantha Rh6AG453500 Rh6BG448100 Rh6CG467300 Rh6DG453900
rosa_wichuraiana Rw6G039520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 155
AclWI GGATC 4 cut(s) 193, 449, 462, 644
AfaI GTAC 2 cut(s) 114, 398
AfiI CCNNNNNNNGG 2 cut(s) 451, 487
AgsI TTSAA 5 cut(s) 252, 432, 466, 569, 620
AluBI AGCT 6 cut(s) 160, 182, 289, 427, 623, 664
AluI AGCT 6 cut(s) 160, 182, 289, 427, 623, 664
Alw21I GWGCWC 1 cut(s) 79
Alw26I GTCTC 1 cut(s) 573
AlwI GGATC 4 cut(s) 193, 449, 462, 644
AlwNI CAGNNNCTG 1 cut(s) 116
ApeKI GCWGC 1 cut(s) 157
Asp700I GAANNNNTTC 1 cut(s) 59
AsuC2I CCSGG 2 cut(s) 351, 452
AsuHPI GGTGA 5 cut(s) 313, 352, 470, 502, 599
BamHI GGATCC 1 cut(s) 454
BarI GAAGNNNNNNTAC 2 cut(s) 47, 79
Bbv12I GWGCWC 1 cut(s) 79
BbvI GCAGC 1 cut(s) 169
BccI CCATC 1 cut(s) 757
BclI TGATCA 1 cut(s) 492
BcnI CCSGG 2 cut(s) 351, 452
BcoDI GTCTC 1 cut(s) 573
BfaI CTAG 2 cut(s) 264, 530
BfmI CTRYAG 1 cut(s) 64
BisI GCNGC 1 cut(s) 158
BlsI GCNGC 1 cut(s) 159
Bme1390I CCNGG 2 cut(s) 351, 452
BmiI GGNNCC 1 cut(s) 456
BmrFI CCNGG 2 cut(s) 351, 452
BmrI ACTGGG 1 cut(s) 120
BmuI ACTGGG 1 cut(s) 120
Bpu10I CCTNAGC 1 cut(s) 290
BpuEI CTTGAG 1 cut(s) 688
BpuMI CCSGG 2 cut(s) 351, 452
BsaJI CCNNGG 1 cut(s) 50
Bsc4I CCNNNNNNNGG 2 cut(s) 451, 487
Bse1I ACTGG 6 cut(s) 46, 110, 126, 202, 587, 653
Bse3DI GCAATG 1 cut(s) 741
BseDI CCNNGG 1 cut(s) 50
BseGI GGATG 4 cut(s) 399, 517, 591, 749
BseLI CCNNNNNNNGG 2 cut(s) 451, 487
BseMI GCAATG 1 cut(s) 741
BseMII CTCAG 3 cut(s) 83, 384, 672
BseNI ACTGG 6 cut(s) 46, 110, 126, 202, 587, 653
BseXI GCAGC 1 cut(s) 169
BsiHKAI GWGCWC 1 cut(s) 79
BsiSI CCGG 2 cut(s) 350, 452
BslFI GGGAC 1 cut(s) 726
BslI CCNNNNNNNGG 2 cut(s) 451, 487
BsmAI GTCTC 1 cut(s) 573
BsmFI GGGAC 1 cut(s) 726
Bsp1286I GDGCHC 1 cut(s) 79
Bsp143I GATC 4 cut(s) 198, 454, 492, 649
BspACI CCGC 1 cut(s) 155
BspCNI CTCAG 3 cut(s) 84, 383, 673
BspLI GGNNCC 1 cut(s) 456
BspPI GGATC 4 cut(s) 193, 449, 462, 644
BspQI GCTCTTC 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 741
BsrI ACTGG 6 cut(s) 46, 110, 126, 202, 587, 653
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 4 cut(s) 198, 454, 492, 649
Bst4CI ACNGT 2 cut(s) 83, 482
Bst6I CTCTTC 3 cut(s) 150, 167, 630
BstDEI CTNAG 4 cut(s) 92, 290, 370, 681
BstF5I GGATG 4 cut(s) 399, 517, 591, 749
BstKTI GATC 4 cut(s) 201, 457, 495, 652
BstMAI GTCTC 1 cut(s) 573
BstMBI GATC 4 cut(s) 198, 454, 492, 649
BstMWI GCNNNNNNNGC 1 cut(s) 55
BstSCI CCNGG 2 cut(s) 349, 450
BstSFI CTRYAG 1 cut(s) 64
BstV1I GCAGC 1 cut(s) 169
BstX2I RGATCY 3 cut(s) 198, 454, 649
BstXI CCANNNNNNTGG 1 cut(s) 133
BstYI RGATCY 3 cut(s) 198, 454, 649
BtsCI GGATG 4 cut(s) 399, 517, 591, 749
BtsIMutI CAGTG 2 cut(s) 39, 378
CaiI CAGNNNCTG 1 cut(s) 116
CseI GACGC 1 cut(s) 747
Csp6I GTAC 2 cut(s) 113, 397
CviAII CATG 2 cut(s) 131, 391
CviQI GTAC 2 cut(s) 113, 397
DdeI CTNAG 4 cut(s) 92, 290, 370, 681
DpnI GATC 4 cut(s) 200, 456, 494, 651
DpnII GATC 4 cut(s) 198, 454, 492, 649
Eam1104I CTCTTC 3 cut(s) 150, 167, 630
EarI CTCTTC 3 cut(s) 150, 167, 630
FaeI CATG 2 cut(s) 134, 394
FalI AAGNNNNNCTT 2 cut(s) 411, 443
FaqI GGGAC 1 cut(s) 726
FatI CATG 2 cut(s) 130, 390
FauI CCCGC 1 cut(s) 162
FbaI TGATCA 1 cut(s) 492
Fnu4HI GCNGC 1 cut(s) 158
FokI GGATG 4 cut(s) 386, 524, 598, 736
Fsp4HI GCNGC 1 cut(s) 158
FspBI CTAG 2 cut(s) 264, 530
GluI GCNGC 1 cut(s) 158
HapII CCGG 2 cut(s) 350, 452
HgaI GACGC 1 cut(s) 747
Hin1II CATG 2 cut(s) 134, 394
HinfI GANTC 2 cut(s) 98, 604
HpaII CCGG 2 cut(s) 350, 452
HphI GGTGA 5 cut(s) 313, 352, 470, 502, 599
Hpy166II GTNNAC 3 cut(s) 19, 39, 700
Hpy188I TCNGA 6 cut(s) 54, 93, 175, 240, 273, 725
Hpy188III TCNNGA 2 cut(s) 530, 667
Hpy8I GTNNAC 3 cut(s) 19, 39, 700
Hpy99I CGWCG 1 cut(s) 486
HpyAV CCTTC 3 cut(s) 583, 680, 688
HpyCH4III ACNGT 2 cut(s) 83, 482
HpyCH4V TGCA 3 cut(s) 192, 209, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 55
HpyF3I CTNAG 4 cut(s) 92, 290, 370, 681
Hsp92II CATG 2 cut(s) 134, 394
Ksp22I TGATCA 1 cut(s) 492
Kzo9I GATC 4 cut(s) 198, 454, 492, 649
LguI GCTCTTC 1 cut(s) 167
LmnI GCTCC 2 cut(s) 286, 751
Lsp1109I GCAGC 1 cut(s) 169
MaeI CTAG 2 cut(s) 264, 530
MaeIII GTNAC 5 cut(s) 83, 95, 301, 340, 655
MalI GATC 4 cut(s) 200, 456, 494, 651
MboI GATC 4 cut(s) 198, 454, 492, 649
MboII GAAGA 4 cut(s) 137, 154, 581, 647
MflI RGATCY 3 cut(s) 198, 454, 649
MhlI GDGCHC 1 cut(s) 79
MluCI AATT 3 cut(s) 385, 406, 729
MlyI GAGTC 1 cut(s) 92
MnlI CCTC 6 cut(s) 60, 99, 178, 468, 631, 676
MroXI GAANNNNTTC 1 cut(s) 59
MseI TTAA 1 cut(s) 335
MslI CAYNNNNRTG 2 cut(s) 272, 754
MspI CCGG 2 cut(s) 350, 452
MspR9I CCNGG 2 cut(s) 351, 452
MwoI GCNNNNNNNGC 1 cut(s) 55
NciI CCSGG 2 cut(s) 351, 452
NdeII GATC 4 cut(s) 198, 454, 492, 649
NlaIII CATG 2 cut(s) 134, 394
NlaIV GGNNCC 1 cut(s) 456
NmuCI GTSAC 3 cut(s) 95, 301, 340
PciSI GCTCTTC 1 cut(s) 167
PdmI GAANNNNTTC 1 cut(s) 59
PfeI GAWTC 1 cut(s) 604
PfoI TCCNGGA 1 cut(s) 450
PkrI GCNGC 1 cut(s) 159
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
PspN4I GGNNCC 1 cut(s) 456
PstNI CAGNNNCTG 1 cut(s) 116
PsuI RGATCY 3 cut(s) 198, 454, 649
RsaI GTAC 2 cut(s) 114, 398
RsaNI GTAC 2 cut(s) 113, 397
RseI CAYNNNNRTG 2 cut(s) 272, 754
SapI GCTCTTC 1 cut(s) 167
SaqAI TTAA 1 cut(s) 335
SatI GCNGC 1 cut(s) 158
Sau3AI GATC 4 cut(s) 198, 454, 492, 649
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 2 cut(s) 351, 452
SduI GDGCHC 1 cut(s) 79
SfcI CTRYAG 1 cut(s) 64
SmiMI CAYNNNNRTG 2 cut(s) 272, 754
SmlI CTYRAG 1 cut(s) 667
SmoI CTYRAG 1 cut(s) 667
Sse9I AATT 3 cut(s) 385, 406, 729
SsiI CCGC 1 cut(s) 155
SspMI CTAG 2 cut(s) 264, 530
StyD4I CCNGG 2 cut(s) 349, 450
TaaI ACNGT 2 cut(s) 83, 482
TaqI TCGA 1 cut(s) 642
TasI AATT 3 cut(s) 385, 406, 729
TatI WGTACW 1 cut(s) 396
TfiI GAWTC 1 cut(s) 604
Tru1I TTAA 1 cut(s) 335
Tru9I TTAA 1 cut(s) 335
TscAI CASTG 2 cut(s) 46, 378
TseFI GTSAC 3 cut(s) 95, 301, 340
TseI GCWGC 1 cut(s) 157
Tsp45I GTSAC 3 cut(s) 95, 301, 340
TspDTI ATGAA 4 cut(s) 137, 192, 407, 602
TspRI CASTG 2 cut(s) 46, 378
XbaI TCTAGA 1 cut(s) 529
XmnI GAANNNNTTC 1 cut(s) 59
XspI CTAG 2 cut(s) 264, 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.