FvH4_2g36340

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012895
Physical Location & Seq
Reverse (-)
26612770 .. 26614649
1880 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36340.t1

Sequence Viewer

Length: 759 bp
ATGTTGGAAAACAAAGTACAGTCAACTCATAGCAAGTCCACTGGTCTGCCTCGGAAGCGGTTCTACCGAGCAAGAGCACACAGTAACCCTCTGAGTGACTCTCACTTTCCAGTACCTGTCGCCCCAAGTCATGTGGATTACTCTCTTCATTATCCGCAGCTCTTCCCATCATCTGATGTAGCTGGTAGTTGCAAAAAGATTCAGTTTGCAGATATTGGCTGTGGTTTTGGGGGGCTTCTGATTAGCCTGTCAACTCTTTTCCCCGAGACTCTGATGATTGGAATGGAGCTTAGGGATAAGGTGACAGAGTATGTCAAGGAAAGGATTTTGGCTCTAAGGGTGACAAACCCAGGTCAATACCAGAATATCTCCGTGGTTCGCACTAATTCCATGAAGTACATCCCAAACTACTTTGAGAAAGCACAGCTGTCAAAGATGTTTTTCTTGTTCCCAGATCCTCACTTCAAAGAGAAAAACCATCGTCGTAGGGTGATCAGTCCATTCCTGCTGGATGAGTATGCTTATGCTCTAGAAGTTGGTGGCATCATTTACACAATCACTGATGTTGAAGAACTCGGAGAGTGGATGAAGACTTGTTTAGAGAATCACCCTATGTTTGAAGCTCTCACAAGTGAGGAGCTCGAAGCAGATCCAGTCACAGAGCTCTTGAGTTCTGCAACCGAGGAAGGACAGAAGGTTGACAGAAACGGGGGACAGACTTTCCGAGCAATTTACAGACGCATTGCTCCATCCCTATGA

Protein Analysis

253

Amino Acids

28.69

Weight (kDa)

6.72

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_4 PF02390 67 - 242 1.7e-50 Putative methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012895)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24840
fragaria_vesca FvH4_2g36340
malus_domestica MD08G1121000.v1.1 MD15G1100000.v1.1
prunus_persica Prupe.1G454800_v2.0.a1
pyrus_communis pycom08g10110 pycom15g09200
rosa_chinensis RchiOBHm_Chr6g0305541
rosa_laevigata RLG00000010878
rosa_multiflora Rmu_co8330221.1_g000001 Rmu_sc0000841.1_g000004
rosa_roxburghii Rroxscaffold_7G00162600
rosa_rugosa Rorug06G0341500
rosa_samantha Rh6AG453500 Rh6BG448100 Rh6CG467300 Rh6DG453900
rosa_wichuraiana Rw6G039520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 58, 155
AclWI GGATC 2 cut(s) 449, 644
AfaI GTAC 3 cut(s) 18, 114, 398
AgsI TTSAA 3 cut(s) 466, 569, 620
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 7 cut(s) 160, 182, 289, 427, 623, 640, 664
AluI AGCT 7 cut(s) 160, 182, 289, 427, 623, 640, 664
Alw21I GWGCWC 3 cut(s) 79, 642, 666
Alw26I GTCTC 1 cut(s) 260
AlwI GGATC 2 cut(s) 449, 644
AlwNI CAGNNNCTG 1 cut(s) 116
Ama87I CYCGRG 1 cut(s) 263
ApeKI GCWGC 1 cut(s) 157
Asp700I GAANNNNTTC 1 cut(s) 59
AsuHPI GGTGA 4 cut(s) 313, 352, 502, 599
AvaI CYCGRG 1 cut(s) 263
BanII GRGCYC 2 cut(s) 642, 666
BarI GAAGNNNNNNTAC 2 cut(s) 47, 79
BbsI GAAGAC 1 cut(s) 596
Bbv12I GWGCWC 3 cut(s) 79, 642, 666
BbvI GCAGC 1 cut(s) 169
BccI CCATC 3 cut(s) 175, 486, 757
BciT130I CCWGG 1 cut(s) 351
BclI TGATCA 1 cut(s) 492
BcoDI GTCTC 1 cut(s) 260
BfaI CTAG 1 cut(s) 530
BisI GCNGC 1 cut(s) 158
BlsI GCNGC 1 cut(s) 159
Bme1390I CCNGG 1 cut(s) 351
BmeT110I CYCGRG 1 cut(s) 263
BmrFI CCNGG 1 cut(s) 351
BmsI GCATC 1 cut(s) 552
BpiI GAAGAC 1 cut(s) 596
BplI GAGNNNNNCTC 2 cut(s) 85, 117
Bpu10I CCTNAGC 1 cut(s) 290
BpuEI CTTGAG 1 cut(s) 688
BsaJI CCNNGG 4 cut(s) 50, 349, 372, 681
Bse1I ACTGG 3 cut(s) 46, 110, 653
Bse3DI GCAATG 1 cut(s) 741
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 4 cut(s) 50, 349, 372, 681
BseGI GGATG 4 cut(s) 399, 517, 591, 749
BseMI GCAATG 1 cut(s) 741
BseMII CTCAG 1 cut(s) 83
BseNI ACTGG 3 cut(s) 46, 110, 653
BseRI GAGGAG 1 cut(s) 650
BseXI GCAGC 1 cut(s) 169
BsiHKAI GWGCWC 3 cut(s) 79, 642, 666
BsiHKCI CYCGRG 1 cut(s) 263
BslFI GGGAC 1 cut(s) 726
BsmAI GTCTC 1 cut(s) 260
BsmFI GGGAC 1 cut(s) 726
BsoBI CYCGRG 1 cut(s) 263
Bsp1286I GDGCHC 3 cut(s) 79, 642, 666
Bsp143I GATC 3 cut(s) 454, 492, 649
BspACI CCGC 2 cut(s) 58, 155
BspCNI CTCAG 1 cut(s) 84
BspPI GGATC 2 cut(s) 449, 644
BspQI GCTCTTC 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 741
BsrI ACTGG 3 cut(s) 46, 110, 653
BssECI CCNNGG 4 cut(s) 50, 349, 372, 681
BssMI GATC 3 cut(s) 454, 492, 649
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 2 cut(s) 21, 83
Bst6I CTCTTC 2 cut(s) 150, 167
BstDEI CTNAG 3 cut(s) 92, 290, 335
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 4 cut(s) 399, 517, 591, 749
BstKTI GATC 3 cut(s) 457, 495, 652
BstMAI GTCTC 1 cut(s) 260
BstMBI GATC 3 cut(s) 454, 492, 649
BstMWI GCNNNNNNNGC 1 cut(s) 55
BstNI CCWGG 1 cut(s) 351
BstSCI CCNGG 1 cut(s) 349
BstV1I GCAGC 1 cut(s) 169
BstV2I GAAGAC 1 cut(s) 596
BstX2I RGATCY 2 cut(s) 454, 649
BstYI RGATCY 2 cut(s) 454, 649
BtgI CCRYGG 1 cut(s) 372
BtsCI GGATG 4 cut(s) 399, 517, 591, 749
BtsIMutI CAGTG 2 cut(s) 39, 558
CaiI CAGNNNCTG 1 cut(s) 116
CseI GACGC 1 cut(s) 747
Csp6I GTAC 3 cut(s) 17, 113, 397
CspCI CAANNNNNGTGG 2 cut(s) 114, 149
CviAII CATG 2 cut(s) 131, 391
CviQI GTAC 3 cut(s) 17, 113, 397
DdeI CTNAG 3 cut(s) 92, 290, 335
DpnI GATC 3 cut(s) 456, 494, 651
DpnII GATC 3 cut(s) 454, 492, 649
Eam1104I CTCTTC 2 cut(s) 150, 167
EarI CTCTTC 2 cut(s) 150, 167
Ecl136II GAGCTC 2 cut(s) 640, 664
Eco24I GRGCYC 2 cut(s) 642, 666
Eco53kI GAGCTC 2 cut(s) 640, 664
Eco88I CYCGRG 1 cut(s) 263
EcoICRI GAGCTC 2 cut(s) 640, 664
EcoRII CCWGG 1 cut(s) 349
EcoT38I GRGCYC 2 cut(s) 642, 666
FaeI CATG 2 cut(s) 134, 394
FaiI YATR 8 cut(s) 30, 132, 312, 392, 519, 525, 614, 757
FaqI GGGAC 1 cut(s) 726
FatI CATG 2 cut(s) 130, 390
FbaI TGATCA 1 cut(s) 492
Fnu4HI GCNGC 1 cut(s) 158
FokI GGATG 4 cut(s) 386, 524, 598, 736
FriOI GRGCYC 2 cut(s) 642, 666
Fsp4HI GCNGC 1 cut(s) 158
FspBI CTAG 1 cut(s) 530
GluI GCNGC 1 cut(s) 158
HgaI GACGC 1 cut(s) 747
Hin1II CATG 2 cut(s) 134, 394
HincII GTYRAC 3 cut(s) 24, 252, 700
HindII GTYRAC 3 cut(s) 24, 252, 700
HinfI GANTC 4 cut(s) 98, 199, 268, 604
HphI GGTGA 4 cut(s) 313, 352, 502, 599
Hpy166II GTNNAC 4 cut(s) 24, 39, 252, 700
Hpy188I TCNGA 7 cut(s) 54, 93, 175, 240, 273, 578, 725
Hpy188III TCNNGA 2 cut(s) 530, 667
Hpy8I GTNNAC 4 cut(s) 24, 39, 252, 700
Hpy99I CGWCG 1 cut(s) 486
HpyAV CCTTC 2 cut(s) 680, 688
HpyCH4III ACNGT 2 cut(s) 21, 83
HpyCH4V TGCA 3 cut(s) 192, 209, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 55
HpyF3I CTNAG 3 cut(s) 92, 290, 335
Hsp92II CATG 2 cut(s) 134, 394
Ksp22I TGATCA 1 cut(s) 492
Kzo9I GATC 3 cut(s) 454, 492, 649
LguI GCTCTTC 1 cut(s) 167
LmnI GCTCC 3 cut(s) 286, 637, 751
Lsp1109I GCAGC 1 cut(s) 169
LweI GCATC 1 cut(s) 552
MaeI CTAG 1 cut(s) 530
MaeIII GTNAC 5 cut(s) 83, 95, 301, 340, 655
MalI GATC 3 cut(s) 456, 494, 651
MboI GATC 3 cut(s) 454, 492, 649
MboII GAAGA 4 cut(s) 137, 154, 581, 601
MflI RGATCY 2 cut(s) 454, 649
MhlI GDGCHC 3 cut(s) 79, 642, 666
MluCI AATT 2 cut(s) 385, 729
MlyI GAGTC 2 cut(s) 92, 262
MnlI CCTC 5 cut(s) 60, 99, 468, 628, 676
MroXI GAANNNNTTC 1 cut(s) 59
MslI CAYNNNNRTG 1 cut(s) 754
MspA1I CMGCKG 1 cut(s) 427
MspR9I CCNGG 1 cut(s) 351
MvaI CCWGG 1 cut(s) 351
MwoI GCNNNNNNNGC 1 cut(s) 55
NdeII GATC 3 cut(s) 454, 492, 649
NlaIII CATG 2 cut(s) 134, 394
NmuCI GTSAC 4 cut(s) 95, 301, 340, 655
PciSI GCTCTTC 1 cut(s) 167
PdmI GAANNNNTTC 1 cut(s) 59
PfeI GAWTC 2 cut(s) 199, 604
PkrI GCNGC 1 cut(s) 159
PleI GAGTC 2 cut(s) 92, 262
PpsI GAGTC 2 cut(s) 92, 262
Psp124BI GAGCTC 2 cut(s) 642, 666
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PstNI CAGNNNCTG 1 cut(s) 116
PsuI RGATCY 2 cut(s) 454, 649
PvuII CAGCTG 1 cut(s) 427
RsaI GTAC 3 cut(s) 18, 114, 398
RsaNI GTAC 3 cut(s) 17, 113, 397
RseI CAYNNNNRTG 1 cut(s) 754
SacI GAGCTC 2 cut(s) 642, 666
SapI GCTCTTC 1 cut(s) 167
SatI GCNGC 1 cut(s) 158
Sau3AI GATC 3 cut(s) 454, 492, 649
SchI GAGTC 2 cut(s) 92, 262
ScrFI CCNGG 1 cut(s) 351
SduI GDGCHC 3 cut(s) 79, 642, 666
SfaNI GCATC 1 cut(s) 552
SmiMI CAYNNNNRTG 1 cut(s) 754
SmlI CTYRAG 1 cut(s) 667
SmoI CTYRAG 1 cut(s) 667
Sse9I AATT 2 cut(s) 385, 729
SsiI CCGC 2 cut(s) 58, 155
SspMI CTAG 1 cut(s) 530
SstI GAGCTC 2 cut(s) 642, 666
StyD4I CCNGG 1 cut(s) 349
TaaI ACNGT 2 cut(s) 21, 83
TaqI TCGA 1 cut(s) 642
TasI AATT 2 cut(s) 385, 729
TatI WGTACW 2 cut(s) 16, 396
TfiI GAWTC 2 cut(s) 199, 604
TscAI CASTG 2 cut(s) 46, 565
TseFI GTSAC 4 cut(s) 95, 301, 340, 655
TseI GCWGC 1 cut(s) 157
Tsp45I GTSAC 4 cut(s) 95, 301, 340, 655
TspDTI ATGAA 3 cut(s) 137, 407, 602
TspGWI ACGGA 1 cut(s) 361
TspRI CASTG 2 cut(s) 46, 565
XbaI TCTAGA 1 cut(s) 529
XmnI GAANNNNTTC 1 cut(s) 59
XspI CTAG 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.