AT5G25080

Sas10 Utp3 C1D (InterPro IPR007146), Exosome-associated factor Rrp47 DNA strand repair C1D (InterPro IPR011082)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010385
Physical Location & Seq
Forward (+)
8643662 .. 8647058
3397 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25080.1

Sequence Viewer

Length: 654 bp
ATGGAAGGAGGTGGAGGAAGTAGCGGCGGAGGAGCTGGAGGAGTGGTTCCGGAATCAGCGATTGAAGCGGTGAATCAAACACTTGCGTACCTCAAGGAACTGAAGCCACAGCTTGAGCAGATGCTGACTTTAGCTGAGCCAGAGGTTCTCGCTGCAATGCAACCATTACAGCGAGCTAAGACTATGCACTTGCTAGCTGAAGCCACCACTACTCTTTACGAACTGAGGTTGAGATGTACTGGTGTTGATCCTGATGATCATCGTGTAAAATCTGAAATTGAGCGGATAAACGTGTACAGAGAGAAGTTTCAGAAGTGTGTTGATCAAAGTAAAGGACCTTTGCGCCCGACCACTGTCTTAAACCGTCAGGCAGCAACCCGTTTCATCGAACATTCTCTACCTGATCTTACATCGACCCAAAAGCAAAGCATTAGAGACCTCAGTAAAGGAGAGAAATCGAGGATCAGGTATTCTGAAACATCTGCGAGAAAGAGGAAATACCAATCAAATGAGAAACAGTCTGTTCAGTCAGCTGCGAAGGACTTTCTTGAAAAGGCAGCTCGTGAGATTATCGGTCACAATGAAAATGGTTTGAAGGGCCCTTTAGTAGCGGCAGCTGATGGTTCAGATGATGTCGAAGTTGGTACAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Sas10_Utp3

Protein Analysis

217

Amino Acids

23.75

Weight (kDa)

7.76

Isoelectric Point (pI)

45.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sas10_Utp3 PF04000 26 - 102 1.7e-10 Sas10/Utp3/C1D family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 283
AccIII TCCGGA 1 cut(s) 49
AciI CCGC 5 cut(s) 24, 27, 68, 283, 611
AclWI GGATC 2 cut(s) 242, 470
AcuI CTGAAG 2 cut(s) 122, 219
AfaI GTAC 4 cut(s) 89, 238, 296, 646
AflIII ACRYGT 1 cut(s) 291
AgsI TTSAA 3 cut(s) 65, 551, 595
AloI GAACNNNNNNTCC 2 cut(s) 30, 62
AluBI AGCT 8 cut(s) 35, 112, 134, 176, 197, 533, 560, 617
AluI AGCT 8 cut(s) 35, 112, 134, 176, 197, 533, 560, 617
Alw26I GTCTC 1 cut(s) 429
AlwI GGATC 2 cut(s) 242, 470
AlwNI CAGNNNCTG 1 cut(s) 124
Aor13HI TCCGGA 1 cut(s) 49
AoxI GGCC 1 cut(s) 598
ApaI GGGCCC 1 cut(s) 602
ApeKI GCWGC 5 cut(s) 152, 371, 533, 557, 614
AspLEI GCGC 1 cut(s) 345
AspS9I GGNCC 3 cut(s) 335, 598, 599
AsuHPI GGTGA 1 cut(s) 82
AsuNHI GCTAGC 1 cut(s) 193
AvaII GGWCC 1 cut(s) 335
BaeGI GKGCMC 1 cut(s) 602
BaeI ACNNNNGTAYC 2 cut(s) 71, 104
BanII GRGCYC 1 cut(s) 602
BauI CACGAG 1 cut(s) 561
BbvI GCAGC 5 cut(s) 139, 383, 520, 569, 626
BccI CCATC 1 cut(s) 614
BclI TGATCA 2 cut(s) 256, 322
BcoDI GTCTC 1 cut(s) 429
BfaI CTAG 1 cut(s) 194
BisI GCNGC 7 cut(s) 25, 153, 372, 534, 558, 612, 615
BlpI GCTNAGC 1 cut(s) 135
BlsI GCNGC 7 cut(s) 26, 154, 373, 535, 559, 613, 616
Bme18I GGWCC 1 cut(s) 335
BmgT120I GGNCC 3 cut(s) 335, 598, 599
BmiI GGNNCC 2 cut(s) 48, 600
BmsI GCATC 1 cut(s) 111
BmtI GCTAGC 1 cut(s) 197
BoxI GACNNNNGTC 1 cut(s) 353
BpmI CTGGAG 1 cut(s) 57
Bpu1102I GCTNAGC 1 cut(s) 135
BpuEI CTTGAG 2 cut(s) 77, 134
BsaBI GATNNNNATC 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 429
BsaWI WCCGGW 1 cut(s) 49
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bse1I ACTGG 1 cut(s) 244
Bse3DI GCAATG 1 cut(s) 162
Bse8I GATNNNNATC 1 cut(s) 258
BseAI TCCGGA 1 cut(s) 49
BseJI GATNNNNATC 1 cut(s) 258
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 3 cut(s) 126, 215, 454
BseNI ACTGG 1 cut(s) 244
BseRI GAGGAG 2 cut(s) 45, 54
BseSI GKGCMC 1 cut(s) 602
BseXI GCAGC 5 cut(s) 139, 383, 520, 569, 626
BshFI GGCC 1 cut(s) 600
BsiSI CCGG 1 cut(s) 50
BsmAI GTCTC 1 cut(s) 429
BsnI GGCC 1 cut(s) 600
Bso31I GGTCTC 1 cut(s) 429
Bsp120I GGGCCC 1 cut(s) 598
Bsp1286I GDGCHC 1 cut(s) 602
Bsp13I TCCGGA 1 cut(s) 49
Bsp1407I TGTACA 1 cut(s) 294
Bsp143I GATC 5 cut(s) 247, 256, 322, 403, 462
Bsp1720I GCTNAGC 1 cut(s) 135
BspACI CCGC 5 cut(s) 24, 27, 68, 283, 611
BspANI GGCC 1 cut(s) 600
BspCNI CTCAG 3 cut(s) 127, 216, 453
BspEI TCCGGA 1 cut(s) 49
BspLI GGNNCC 2 cut(s) 48, 600
BspOI GCTAGC 1 cut(s) 197
BspPI GGATC 2 cut(s) 242, 470
BspTNI GGTCTC 1 cut(s) 429
BsrBI CCGCTC 1 cut(s) 283
BsrDI GCAATG 1 cut(s) 162
BsrGI TGTACA 1 cut(s) 294
BsrI ACTGG 1 cut(s) 244
BssMI GATC 5 cut(s) 247, 256, 322, 403, 462
BssSI CACGAG 1 cut(s) 561
Bst2BI CACGAG 1 cut(s) 561
Bst4CI ACNGT 3 cut(s) 355, 365, 519
BstAUI TGTACA 1 cut(s) 294
BstC8I GCNNGC 2 cut(s) 174, 195
BstDEI CTNAG 4 cut(s) 135, 177, 224, 440
BstHHI GCGC 1 cut(s) 345
BstKTI GATC 5 cut(s) 250, 259, 325, 406, 465
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 5 cut(s) 247, 256, 322, 403, 462
BstMWI GCNNNNNNNGC 1 cut(s) 65
BstPAI GACNNNNGTC 1 cut(s) 353
BstSLI GKGCMC 1 cut(s) 602
BstV1I GCAGC 5 cut(s) 139, 383, 520, 569, 626
BsuRI GGCC 1 cut(s) 600
BtsIMutI CAGTG 1 cut(s) 351
Cac8I GCNNGC 2 cut(s) 174, 195
CaiI CAGNNNCTG 1 cut(s) 124
CfoI GCGC 1 cut(s) 345
Cfr13I GGNCC 3 cut(s) 335, 598, 599
Csp6I GTAC 4 cut(s) 88, 237, 295, 645
CviQI GTAC 4 cut(s) 88, 237, 295, 645
DdeI CTNAG 4 cut(s) 135, 177, 224, 440
DpnI GATC 5 cut(s) 249, 258, 324, 405, 464
DpnII GATC 5 cut(s) 247, 256, 322, 403, 462
EciI GGCGGA 1 cut(s) 42
Eco24I GRGCYC 1 cut(s) 602
Eco31I GGTCTC 1 cut(s) 429
Eco47I GGWCC 1 cut(s) 335
Eco57I CTGAAG 2 cut(s) 122, 219
EcoO109I RGGNCCY 3 cut(s) 335, 598, 599
EcoT38I GRGCYC 1 cut(s) 602
FaiI YATR 1 cut(s) 185
FbaI TGATCA 2 cut(s) 256, 322
Fnu4HI GCNGC 7 cut(s) 25, 153, 372, 534, 558, 612, 615
FriOI GRGCYC 1 cut(s) 602
Fsp4HI GCNGC 7 cut(s) 25, 153, 372, 534, 558, 612, 615
FspBI CTAG 1 cut(s) 194
GlaI GCGC 1 cut(s) 344
GluI GCNGC 7 cut(s) 25, 153, 372, 534, 558, 612, 615
GsuI CTGGAG 1 cut(s) 57
HaeIII GGCC 1 cut(s) 600
HapII CCGG 1 cut(s) 50
HhaI GCGC 1 cut(s) 345
Hin6I GCGC 1 cut(s) 343
HinP1I GCGC 1 cut(s) 343
HinfI GANTC 2 cut(s) 53, 73
HpaII CCGG 1 cut(s) 50
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 4 cut(s) 274, 312, 475, 628
Hpy188III TCNNGA 4 cut(s) 50, 251, 548, 563
Hpy8I GTNNAC 1 cut(s) 295
HpyAV CCTTC 2 cut(s) 532, 589
HpyCH4III ACNGT 3 cut(s) 355, 365, 519
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 3 cut(s) 155, 160, 187
HpyF10VI GCNNNNNNNGC 1 cut(s) 65
HpyF3I CTNAG 4 cut(s) 135, 177, 224, 440
HpySE526I ACGT 1 cut(s) 291
HspAI GCGC 1 cut(s) 343
Kpn2I TCCGGA 1 cut(s) 49
Ksp22I TGATCA 2 cut(s) 256, 322
Kzo9I GATC 5 cut(s) 247, 256, 322, 403, 462
LmnI GCTCC 1 cut(s) 32
LpnPI CCDG 8 cut(s) 21, 63, 153, 225, 264, 353, 414, 451
Lsp1109I GCAGC 5 cut(s) 139, 383, 520, 569, 626
LweI GCATC 1 cut(s) 111
MaeI CTAG 1 cut(s) 194
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 1 cut(s) 575
MalI GATC 5 cut(s) 249, 258, 324, 405, 464
MbiI CCGCTC 1 cut(s) 283
MboI GATC 5 cut(s) 247, 256, 322, 403, 462
MhlI GDGCHC 1 cut(s) 602
MluCI AATT 1 cut(s) 276
MnlI CCTC 9 cut(s) 8, 23, 32, 101, 136, 219, 449, 453, 486
MroI TCCGGA 1 cut(s) 49
MseI TTAA 1 cut(s) 359
MspA1I CMGCKG 2 cut(s) 533, 617
MspI CCGG 1 cut(s) 50
MwoI GCNNNNNNNGC 1 cut(s) 65
NdeII GATC 5 cut(s) 247, 256, 322, 403, 462
NheI GCTAGC 1 cut(s) 193
NlaIV GGNNCC 2 cut(s) 48, 600
NmuCI GTSAC 1 cut(s) 575
PfeI GAWTC 2 cut(s) 53, 73
PkrI GCNGC 7 cut(s) 26, 154, 373, 535, 559, 613, 616
PpuMI RGGWCCY 1 cut(s) 335
PshAI GACNNNNGTC 1 cut(s) 353
Psp5II RGGWCCY 1 cut(s) 335
PspN4I GGNNCC 2 cut(s) 48, 600
PspOMI GGGCCC 1 cut(s) 598
PspPI GGNCC 3 cut(s) 335, 598, 599
PspPPI RGGWCCY 1 cut(s) 335
PsrI GAACNNNNNNTAC 2 cut(s) 381, 413
PstNI CAGNNNCTG 1 cut(s) 124
PvuII CAGCTG 2 cut(s) 533, 617
RsaI GTAC 4 cut(s) 89, 238, 296, 646
RsaNI GTAC 4 cut(s) 88, 237, 295, 645
SaqAI TTAA 1 cut(s) 359
SatI GCNGC 7 cut(s) 25, 153, 372, 534, 558, 612, 615
Sau3AI GATC 5 cut(s) 247, 256, 322, 403, 462
Sau96I GGNCC 3 cut(s) 335, 598, 599
SduI GDGCHC 1 cut(s) 602
SfaNI GCATC 1 cut(s) 111
SinI GGWCC 1 cut(s) 335
SmlI CTYRAG 2 cut(s) 92, 113
SmoI CTYRAG 2 cut(s) 92, 113
Sse9I AATT 1 cut(s) 276
SsiI CCGC 5 cut(s) 24, 27, 68, 283, 611
SspMI CTAG 1 cut(s) 194
TaaI ACNGT 3 cut(s) 355, 365, 519
TaiI ACGT 1 cut(s) 294
TaqI TCGA 4 cut(s) 387, 413, 458, 636
TaqII GACCGA 1 cut(s) 563
TasI AATT 1 cut(s) 276
TatI WGTACW 2 cut(s) 236, 294
TauI GCSGC 2 cut(s) 27, 614
TfiI GAWTC 2 cut(s) 53, 73
Tru1I TTAA 1 cut(s) 359
Tru9I TTAA 1 cut(s) 359
TscAI CASTG 1 cut(s) 358
TseFI GTSAC 1 cut(s) 575
TseI GCWGC 5 cut(s) 152, 371, 533, 557, 614
Tsp45I GTSAC 1 cut(s) 575
TspDTI ATGAA 2 cut(s) 373, 597
TspRI CASTG 1 cut(s) 358
VpaK11BI GGWCC 1 cut(s) 335
XspI CTAG 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.