MD08G1055900.v1.1

Nuclear nucleic acid-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010385
Physical Location & Seq
Forward (+)
4337057 .. 4340299
3243 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1055900.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGAAGGAAGCTCGATAATACCAGATTCAGTGATGGAATCAGTGAAGAAAACCTTGGGGGACGTCGAAGAACTTCGACCCCACCTCCTCCAGTTCCTCTCCCTCGCCGACGCCGACGTCCTCGCCGAAATGCCGCCGCTCGAGCGCGCCCACTCGCTGCTTATGCTCTCCAAGGCCACCACCCTCCTTTTTGCCTTGAGGCTGAGGTGCACTGGAGTAAACCCATTTAACCATCCAGTCAAAACGGAGTTCGATAGATTAAAATTGTATGAGGAGAAGCTTGAGAAGTTGGTGGATTTGAGTAAAGCACCCTTGCGACCATCCACTACTTTAAATCATCAGGCAGCTACCCGTTTCATCGAGCATTCTTTGCCCGACCTCACCCCTGATCAGAGGCAAAGTATGAGAGCCATTAGCAAAGGAGATGGGCCAACCATGAAGTATCTAGAAAGGAATGCACCAAAGAAAAGGAAATATCAATCTCCTAATAAACAATCAGTTCAATCTGCTGCCAAGGAATTCCTTGAGAAAGCAGCACGAGAGATACTTGGTGATAATGAAGGTGGTATGAAGGGACCTATACAGATCAGTTCAGATGAAGAGGACGGTTGCGTTGCGGAGACCAGATTTGCTCTCGTTTTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Sas10_Utp3

Protein Analysis

216

Amino Acids

24.03

Weight (kDa)

6.25

Isoelectric Point (pI)

48.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sas10_Utp3 PF04000 21 - 93 2.5e-10 Sas10/Utp3/C1D family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 116
AatII GACGTC 2 cut(s) 66, 120
AccBSI CCGCTC 1 cut(s) 139
AccII CGCG 1 cut(s) 147
AciI CCGC 3 cut(s) 134, 137, 617
AcsI RAATTY 1 cut(s) 518
AcyI GRCGYC 3 cut(s) 63, 111, 117
AgsI TTSAA 1 cut(s) 503
AjuI GAANNNNNNNTTGG 2 cut(s) 38, 70
AluBI AGCT 3 cut(s) 12, 280, 347
AluI AGCT 3 cut(s) 12, 280, 347
Alw21I GWGCWC 1 cut(s) 212
Alw26I GTCTC 1 cut(s) 614
Alw44I GTGCAC 1 cut(s) 208
Ama87I CYCGRG 1 cut(s) 140
AoxI GGCC 2 cut(s) 174, 428
ApaLI GTGCAC 1 cut(s) 208
ApeKI GCWGC 4 cut(s) 157, 344, 509, 533
ApoI RAATTY 1 cut(s) 518
Asp700I GAANNNNTTC 1 cut(s) 72
AspLEI GCGC 2 cut(s) 147, 149
AspS9I GGNCC 2 cut(s) 428, 575
AsuHPI GGTGA 2 cut(s) 373, 563
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 1 cut(s) 575
BaeGI GKGCMC 1 cut(s) 212
BauI CACGAG 1 cut(s) 537
Bbv12I GWGCWC 1 cut(s) 212
BbvCI CCTCAGC 1 cut(s) 203
BbvI GCAGC 4 cut(s) 144, 356, 496, 545
BccI CCATC 4 cut(s) 28, 240, 328, 419
BclI TGATCA 1 cut(s) 388
BcoDI GTCTC 1 cut(s) 614
BfaI CTAG 1 cut(s) 446
BisI GCNGC 6 cut(s) 134, 137, 158, 345, 510, 534
BlsI GCNGC 6 cut(s) 135, 138, 159, 346, 511, 535
Bme18I GGWCC 1 cut(s) 575
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 2 cut(s) 428, 575
BmiI GGNNCC 1 cut(s) 576
BpmI CTGGAG 2 cut(s) 74, 234
Bpu10I CCTNAGC 1 cut(s) 203
BpuEI CTTGAG 3 cut(s) 217, 302, 545
BsaHI GRCGYC 3 cut(s) 63, 111, 117
BsaI GGTCTC 1 cut(s) 614
BsaJI CCNNGG 3 cut(s) 54, 171, 513
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bse1I ACTGG 3 cut(s) 91, 217, 236
BseDI CCNNGG 3 cut(s) 54, 171, 513
BseGI GGATG 2 cut(s) 232, 320
BseMII CTCAG 1 cut(s) 194
BseNI ACTGG 3 cut(s) 91, 217, 236
BsePI GCGCGC 1 cut(s) 145
BseRI GAGGAG 2 cut(s) 77, 287
BseSI GKGCMC 1 cut(s) 212
BseXI GCAGC 4 cut(s) 144, 356, 496, 545
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 2 cut(s) 176, 430
BsiHKAI GWGCWC 1 cut(s) 212
BsiHKCI CYCGRG 1 cut(s) 140
BslFI GGGAC 2 cut(s) 74, 588
BsmAI GTCTC 1 cut(s) 614
BsmFI GGGAC 2 cut(s) 74, 588
BsmI GAATGC 2 cut(s) 364, 460
BsnI GGCC 2 cut(s) 176, 430
Bso31I GGTCTC 1 cut(s) 614
BsoBI CYCGRG 1 cut(s) 140
Bsp1286I GDGCHC 1 cut(s) 212
Bsp143I GATC 2 cut(s) 388, 585
BspACI CCGC 3 cut(s) 134, 137, 617
BspANI GGCC 2 cut(s) 176, 430
BspCNI CTCAG 1 cut(s) 195
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 1 cut(s) 576
BspTNI GGTCTC 1 cut(s) 614
BsrBI CCGCTC 1 cut(s) 139
BsrI ACTGG 3 cut(s) 91, 217, 236
BssECI CCNNGG 3 cut(s) 54, 171, 513
BssHII GCGCGC 1 cut(s) 145
BssMI GATC 2 cut(s) 388, 585
BssNI GRCGYC 3 cut(s) 63, 111, 117
BssSI CACGAG 1 cut(s) 537
BssT1I CCWWGG 3 cut(s) 54, 171, 513
Bst2BI CACGAG 1 cut(s) 537
Bst4CI ACNGT 1 cut(s) 608
Bst6I CTCTTC 1 cut(s) 594
BstACI GRCGYC 3 cut(s) 63, 111, 117
BstAPI GCANNNNNTGC 1 cut(s) 370
BstC8I GCNNGC 1 cut(s) 147
BstDEI CTNAG 1 cut(s) 203
BstF5I GGATG 2 cut(s) 232, 320
BstFNI CGCG 1 cut(s) 147
BstHHI GCGC 2 cut(s) 147, 149
BstKTI GATC 2 cut(s) 391, 588
BstMAI GTCTC 1 cut(s) 614
BstMBI GATC 2 cut(s) 388, 585
BstMWI GCNNNNNNNGC 3 cut(s) 142, 163, 370
BstSLI GKGCMC 1 cut(s) 212
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 4 cut(s) 144, 356, 496, 545
BsuRI GGCC 2 cut(s) 176, 430
BtsCI GGATG 2 cut(s) 232, 320
BtsIMutI CAGTG 3 cut(s) 36, 48, 210
Cac8I GCNNGC 1 cut(s) 147
CfoI GCGC 2 cut(s) 147, 149
Cfr13I GGNCC 2 cut(s) 428, 575
CseI GACGC 1 cut(s) 119
CviAII CATG 1 cut(s) 436
CviJI RGCY 7 cut(s) 12, 176, 202, 280, 347, 410, 430
CviKI_1 RGCY 7 cut(s) 12, 176, 202, 280, 347, 410, 430
DdeI CTNAG 1 cut(s) 203
DpnI GATC 2 cut(s) 390, 587
DpnII GATC 2 cut(s) 388, 585
DraI TTTAAA 1 cut(s) 333
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Eco130I CCWWGG 3 cut(s) 54, 171, 513
Eco31I GGTCTC 1 cut(s) 614
Eco47I GGWCC 1 cut(s) 575
Eco88I CYCGRG 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 575
EcoRI GAATTC 1 cut(s) 518
EcoT14I CCWWGG 3 cut(s) 54, 171, 513
ErhI CCWWGG 3 cut(s) 54, 171, 513
FaeI CATG 1 cut(s) 439
FaiI YATR 6 cut(s) 164, 270, 404, 437, 569, 581
FalI AAGNNNNNCTT 2 cut(s) 38, 70
FaqI GGGAC 2 cut(s) 74, 588
FatI CATG 1 cut(s) 435
FbaI TGATCA 1 cut(s) 388
Fnu4HI GCNGC 6 cut(s) 134, 137, 158, 345, 510, 534
FokI GGATG 2 cut(s) 219, 307
Fsp4HI GCNGC 6 cut(s) 134, 137, 158, 345, 510, 534
FspBI CTAG 1 cut(s) 446
GlaI GCGC 2 cut(s) 146, 148
GluI GCNGC 6 cut(s) 134, 137, 158, 345, 510, 534
GsuI CTGGAG 2 cut(s) 74, 234
HaeIII GGCC 2 cut(s) 176, 430
HgaI GACGC 1 cut(s) 119
HhaI GCGC 2 cut(s) 147, 149
Hin1I GRCGYC 3 cut(s) 63, 111, 117
Hin1II CATG 1 cut(s) 439
Hin6I GCGC 2 cut(s) 145, 147
HinP1I GCGC 2 cut(s) 145, 147
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 2 cut(s) 26, 38
HphI GGTGA 2 cut(s) 373, 563
Hpy166II GTNNAC 2 cut(s) 210, 220
Hpy188I TCNGA 2 cut(s) 393, 595
Hpy188III TCNNGA 1 cut(s) 446
Hpy8I GTNNAC 2 cut(s) 210, 220
Hpy99I CGWCG 3 cut(s) 68, 113, 119
HpyAV CCTTC 2 cut(s) 554, 565
HpyCH4III ACNGT 1 cut(s) 608
HpyCH4IV ACGT 2 cut(s) 63, 117
HpyCH4V TGCA 2 cut(s) 210, 458
HpyF10VI GCNNNNNNNGC 3 cut(s) 142, 163, 370
HpyF3I CTNAG 1 cut(s) 203
HpySE526I ACGT 2 cut(s) 63, 117
Hsp92I GRCGYC 3 cut(s) 63, 111, 117
Hsp92II CATG 1 cut(s) 439
HspAI GCGC 2 cut(s) 145, 147
Ksp22I TGATCA 1 cut(s) 388
Kzo9I GATC 2 cut(s) 388, 585
LpnPI CCDG 7 cut(s) 36, 104, 198, 249, 326, 399, 637
Lsp1109I GCAGC 4 cut(s) 144, 356, 496, 545
MaeI CTAG 1 cut(s) 446
MaeII ACGT 2 cut(s) 63, 117
MalI GATC 2 cut(s) 390, 587
MbiI CCGCTC 1 cut(s) 139
MboI GATC 2 cut(s) 388, 585
MboII GAAGA 3 cut(s) 58, 80, 611
MhlI GDGCHC 1 cut(s) 212
MluCI AATT 2 cut(s) 263, 518
MroXI GAANNNNTTC 1 cut(s) 72
MseI TTAA 3 cut(s) 228, 260, 332
Mva1269I GAATGC 2 cut(s) 364, 460
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 3 cut(s) 142, 163, 370
NdeII GATC 2 cut(s) 388, 585
NlaIII CATG 1 cut(s) 439
NlaIV GGNNCC 1 cut(s) 576
PaeR7I CTCGAG 1 cut(s) 140
PauI GCGCGC 1 cut(s) 145
PcsI WCGNNNNNNNCGW 2 cut(s) 111, 123
PctI GAATGC 2 cut(s) 364, 460
PdmI GAANNNNTTC 1 cut(s) 72
PfeI GAWTC 2 cut(s) 26, 38
PkrI GCNGC 6 cut(s) 135, 138, 159, 346, 511, 535
PpuMI RGGWCCY 1 cut(s) 575
Psp5II RGGWCCY 1 cut(s) 575
PspN4I GGNNCC 1 cut(s) 576
PspPI GGNCC 2 cut(s) 428, 575
PspPPI RGGWCCY 1 cut(s) 575
PspXI VCTCGAGB 1 cut(s) 140
PteI GCGCGC 1 cut(s) 145
SaqAI TTAA 3 cut(s) 228, 260, 332
SatI GCNGC 6 cut(s) 134, 137, 158, 345, 510, 534
Sau3AI GATC 2 cut(s) 388, 585
Sau96I GGNCC 2 cut(s) 428, 575
SduI GDGCHC 1 cut(s) 212
Sfr274I CTCGAG 1 cut(s) 140
SinI GGWCC 1 cut(s) 575
SlaI CTCGAG 1 cut(s) 140
SmlI CTYRAG 4 cut(s) 140, 196, 281, 524
SmoI CTYRAG 4 cut(s) 140, 196, 281, 524
Sse9I AATT 2 cut(s) 263, 518
SsiI CCGC 3 cut(s) 134, 137, 617
SspMI CTAG 1 cut(s) 446
StyI CCWWGG 3 cut(s) 54, 171, 513
TaaI ACNGT 1 cut(s) 608
TaiI ACGT 2 cut(s) 66, 120
TaqI TCGA 6 cut(s) 14, 66, 76, 141, 252, 360
TasI AATT 2 cut(s) 263, 518
TauI GCSGC 2 cut(s) 136, 139
TfiI GAWTC 2 cut(s) 26, 38
Tru1I TTAA 3 cut(s) 228, 260, 332
Tru9I TTAA 3 cut(s) 228, 260, 332
TscAI CASTG 3 cut(s) 36, 48, 217
TseI GCWGC 4 cut(s) 157, 344, 509, 533
TspDTI ATGAA 5 cut(s) 346, 452, 573, 584, 612
TspGWI ACGGA 1 cut(s) 260
TspRI CASTG 3 cut(s) 36, 48, 217
VneI GTGCAC 1 cut(s) 208
VpaK11BI GGWCC 1 cut(s) 575
XapI RAATTY 1 cut(s) 518
XbaI TCTAGA 1 cut(s) 445
XhoI CTCGAG 1 cut(s) 140
XmnI GAANNNNTTC 1 cut(s) 72
XspI CTAG 1 cut(s) 446
ZraI GACGTC 2 cut(s) 64, 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.