AT5G25550

Leucine-rich repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012723
Physical Location & Seq
Forward (+)
8893989 .. 8895914
1926 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25550.1

Sequence Viewer

Length: 1302 bp
ATGAGGATTTATCAACCAACCTTATTAATATTCACCACCGTCGTCTTACTCTCCATCTCCGCCGTCGCTCCTGGTGGCTCCCGGCAACTTTTGTACACCCGAGACGACCCTATAACCATACCTCCGTACCTAATATTCGAAAACGTACGGCTCGAAAGAGCTTACGTGGCGTTACAAGCGTGGAAACGTGCCATGATCTCTGACCCGTGGAATCTGACAACTAACTGGTTTGGATCACGTGTGTGCGACTACAACGGTGTAGTATGCTCCGAATCTCTCGACGACCCTTTGGTTAAAACTGTCTCCGGTGTTGATCTAAACCAGGGAGATATCGCTGGTCATCTCCCTGAAGAGCTTGGCCTCTTAACCGATATTGCTCTGTTTCACGTTAACTCAAACCGGTTCTGTGGTACACTCCCGGTCGGGTTTTCGCAGTTGAGTCTCCTTTTCGAACTTGATCTTAGTAATAACCGGTTCGCTGGTAAATTCCCGGAAGTGGTAATCGGTTTACCGAAATTAAAGTATCTTGATCTCCGGTACAACGAGTTTGAAGGTGAATTACCAGAATCTTTGTTCGATAAAGATCTTGACGCTTTATTCCTGAATAGTAACCGGTTTAGAAGTAAAATCCCGGTTAACATGGGAAATTCACCGGTATCGGTTCTAGTTCTAGCCTCAAACCGGTTTGAAGGATGTATACCGCCGAGTTTCGGTAAAATGGGTAAGACTCTAAACGAGATCATTCTTATGGATAACGGTTTACAATCTTGTATACCTAATGACATGGGATTACTTCAAAACGTTACGGTTTTGGACATTAGTTATAACTGGTTGGTCGGAGAGTTACCTAAGTCGATGGGTCAGATGGAGAATCTTGAGGTGTTGAACGTTGAACGTAACATGCTCTCCGGTTTAATACCGGATGAGCTATGTTCGCTTGAGAAGCTAAGAGATTTTAGATACGGGTCTAACTATTTCACCGGAGAACCAGCCACGTGTCGCTATCTAGAGAATTATAATTATACGATGAATTGCTTCAAGGACGTGAGGGACCAAAGATCGATGATGGAGTGTAAGATGTTTTTGTCGAAACCTGTAGATTGTGACTCTTTCAAGTGTAGTCCTGGCTCTTCGTGTTTTTCTCCTCCTCCGAGTCAAATATCACCATCTTCGCAACCATTGGCACCGGCGCCATCTCCTACTTCACCACCATTGTCAACACCGCCTCCGGCTAGACCATGTCCGCCGGTATACTCACCACCTCCACCACCACCATTGTCACTGGCGCCGTCTATGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005199 GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8

Protein Analysis

433

Amino Acids

48.09

Weight (kDa)

4.93

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 129 - 182 5.1e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012723)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25550
fragaria_vesca FvH4_2g30240
malus_domestica MD08G1135200.v1.1 MD15G1114000.v1.1
prunus_persica Prupe.1G465200_v2.0.a1
pyrus_communis pycom08g11410 pycom15g10230
rosa_chinensis RchiOBHm_Chr6g0312631
rosa_laevigata RLG00000010309
rosa_multiflora Rmu_sc0002859.1_g000007 Rmu_sc0034244.1_g000001 Rmu_ssc0000412.1_g000003
rosa_roxburghii Rroxscaffold_7G00156630
rosa_rugosa Rorug06G0398000
rosa_samantha Rh6AG515300 Rh6BG524800 Rh6CG530200
rosa_wichuraiana Rw6G044750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 825, 1017
AccB1I GGYRCC 3 cut(s) 1183, 1189, 1285
AccI GTMKAC 3 cut(s) 697, 772, 1251
AciI CCGC 4 cut(s) 60, 701, 1223, 1244
AclI AACGTT 2 cut(s) 801, 888
AclWI GGATC 1 cut(s) 241
AcsI RAATTY 2 cut(s) 485, 646
AcuI CTGAAG 1 cut(s) 369
AcvI CACGTG 2 cut(s) 239, 996
AcyI GRCGYC 2 cut(s) 1190, 1286
AfaI GTAC 5 cut(s) 95, 128, 147, 412, 539
AfiI CCNNNNNNNGG 3 cut(s) 496, 681, 710
AflIII ACRYGT 2 cut(s) 238, 995
AgeI ACCGGT 5 cut(s) 399, 471, 612, 652, 681
AgsI TTSAA 7 cut(s) 551, 689, 797, 886, 893, 1039, 1114
AjiI CACGTC 1 cut(s) 1045
AjnI CCWGG 3 cut(s) 70, 321, 1123
AleI CACNNNNGTG 1 cut(s) 241
AluBI AGCT 4 cut(s) 161, 355, 928, 946
AluI AGCT 4 cut(s) 161, 355, 928, 946
Alw26I GTCTC 3 cut(s) 96, 307, 446
AlwI GGATC 1 cut(s) 241
Ama87I CYCGRG 1 cut(s) 99
AoxI GGCC 1 cut(s) 358
ApoI RAATTY 2 cut(s) 485, 646
AseI ATTAAT 1 cut(s) 26
AsiGI ACCGGT 5 cut(s) 399, 471, 612, 652, 681
Asp700I GAANNNNTTC 1 cut(s) 1034
AspLEI GCGC 2 cut(s) 1192, 1288
AspS9I GGNCC 1 cut(s) 1051
AsuC2I CCSGG 4 cut(s) 82, 419, 491, 632
AsuHPI GGTGA 7 cut(s) 25, 566, 642, 970, 1155, 1197, 1248
AsuII TTCGAA 2 cut(s) 138, 450
AvaI CYCGRG 1 cut(s) 99
AvaII GGWCC 1 cut(s) 1051
BaeI ACNNNNGTAYC 2 cut(s) 110, 143
BanI GGYRCC 3 cut(s) 1183, 1189, 1285
BarI GAAGNNNNNNTAC 4 cut(s) 543, 575, 681, 713
BbrPI CACGTG 2 cut(s) 239, 996
BccI CCATC 6 cut(s) 62, 850, 859, 1060, 1174, 1201
BceAI ACGGC 3 cut(s) 47, 164, 1273
BciT130I CCWGG 3 cut(s) 72, 323, 1125
BcnI CCSGG 4 cut(s) 82, 419, 491, 632
BcoDI GTCTC 3 cut(s) 96, 307, 446
BfaI CTAG 4 cut(s) 665, 671, 1007, 1233
BfmI CTRYAG 1 cut(s) 1095
BfoI RGCGCY 2 cut(s) 1193, 1289
BglII AGATCT 1 cut(s) 583
Bme1390I CCNGG 7 cut(s) 72, 82, 323, 419, 491, 632, 1125
Bme18I GGWCC 1 cut(s) 1051
BmeT110I CYCGRG 1 cut(s) 99
BmgBI CACGTC 1 cut(s) 1045
BmgT120I GGNCC 1 cut(s) 1051
BmiI GGNNCC 5 cut(s) 79, 1052, 1185, 1191, 1287
BmrFI CCNGG 7 cut(s) 72, 82, 323, 419, 491, 632, 1125
Bpu14I TTCGAA 2 cut(s) 138, 450
BpuEI CTTGAG 2 cut(s) 896, 959
BpuMI CCSGG 4 cut(s) 82, 419, 491, 632
Bsa29I ATCGAT 1 cut(s) 1061
BsaAI YACGTR 3 cut(s) 166, 239, 996
BsaBI GATNNNNATC 1 cut(s) 582
BsaHI GRCGYC 2 cut(s) 1190, 1286
BsaJI CCNNGG 2 cut(s) 206, 322
BsaXI ACNNNNNCTCC 6 cut(s) 106, 136, 890, 920, 1210, 1240
Bsc4I CCNNNNNNNGG 3 cut(s) 496, 681, 710
Bse118I RCCGGY 7 cut(s) 399, 471, 612, 652, 681, 1186, 1246
Bse1I ACTGG 3 cut(s) 230, 833, 1287
Bse8I GATNNNNATC 1 cut(s) 582
BseBI CCWGG 3 cut(s) 72, 323, 1125
BseCI ATCGAT 1 cut(s) 1061
BseDI CCNNGG 2 cut(s) 206, 322
BseGI GGATG 2 cut(s) 698, 928
BseJI GATNNNNATC 1 cut(s) 582
BseLI CCNNNNNNNGG 3 cut(s) 496, 681, 710
BseNI ACTGG 3 cut(s) 230, 833, 1287
BseRI GAGGAG 2 cut(s) 1134, 1137
Bsh1285I CGRYCG 1 cut(s) 423
BshFI GGCC 1 cut(s) 360
BshNI GGYRCC 3 cut(s) 1183, 1189, 1285
BshTI ACCGGT 5 cut(s) 399, 471, 612, 652, 681
BshVI ATCGAT 1 cut(s) 1061
BsiEI CGRYCG 1 cut(s) 423
BsiHKCI CYCGRG 1 cut(s) 99
BsiWI CGTACG 1 cut(s) 145
BslFI GGGAC 1 cut(s) 1064
BslI CCNNNNNNNGG 3 cut(s) 496, 681, 710
BsmAI GTCTC 3 cut(s) 96, 307, 446
BsmBI CGTCTC 1 cut(s) 96
BsmFI GGGAC 1 cut(s) 1064
BsnI GGCC 1 cut(s) 360
BsoBI CYCGRG 1 cut(s) 99
Bsp119I TTCGAA 2 cut(s) 138, 450
Bsp1407I TGTACA 1 cut(s) 93
Bsp143I GATC 8 cut(s) 195, 233, 313, 457, 529, 583, 738, 1058
BspACI CCGC 4 cut(s) 60, 701, 1223, 1244
BspANI GGCC 1 cut(s) 360
BspDI ATCGAT 1 cut(s) 1061
BspLI GGNNCC 5 cut(s) 79, 1052, 1185, 1191, 1287
BspPI GGATC 1 cut(s) 241
BspQI GCTCTTC 2 cut(s) 345, 1135
BspT104I TTCGAA 2 cut(s) 138, 450
BspT107I GGYRCC 3 cut(s) 1183, 1189, 1285
BsrFI RCCGGY 7 cut(s) 399, 471, 612, 652, 681, 1186, 1246
BsrGI TGTACA 1 cut(s) 93
BsrI ACTGG 3 cut(s) 230, 833, 1287
BssAI RCCGGY 7 cut(s) 399, 471, 612, 652, 681, 1186, 1246
BssECI CCNNGG 2 cut(s) 206, 322
BssMI GATC 8 cut(s) 195, 233, 313, 457, 529, 583, 738, 1058
BssNAI GTATAC 3 cut(s) 698, 773, 1252
BssNI GRCGYC 2 cut(s) 1190, 1286
Bst1107I GTATAC 3 cut(s) 698, 773, 1252
Bst2UI CCWGG 3 cut(s) 72, 323, 1125
Bst4CI ACNGT 5 cut(s) 40, 257, 301, 758, 808
Bst6I CTCTTC 2 cut(s) 345, 1135
BstACI GRCGYC 2 cut(s) 1190, 1286
BstAUI TGTACA 1 cut(s) 93
BstBAI YACGTR 3 cut(s) 166, 239, 996
BstBI TTCGAA 2 cut(s) 138, 450
BstDEI CTNAG 3 cut(s) 461, 849, 947
BstDSI CCRYGG 1 cut(s) 206
BstF5I GGATG 2 cut(s) 698, 928
BstH2I RGCGCY 2 cut(s) 1193, 1289
BstHHI GCGC 2 cut(s) 1192, 1288
BstKTI GATC 8 cut(s) 198, 236, 316, 460, 532, 586, 741, 1061
BstMAI GTCTC 3 cut(s) 96, 307, 446
BstMBI GATC 8 cut(s) 195, 233, 313, 457, 529, 583, 738, 1058
BstMCI CGRYCG 1 cut(s) 423
BstMWI GCNNNNNNNGC 4 cut(s) 167, 176, 934, 943
BstNI CCWGG 3 cut(s) 72, 323, 1125
BstNSI RCATGY 1 cut(s) 904
BstSCI CCNGG 7 cut(s) 70, 80, 321, 417, 489, 630, 1123
BstSFI CTRYAG 1 cut(s) 1095
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
BstZ17I GTATAC 3 cut(s) 698, 773, 1252
Bsu15I ATCGAT 1 cut(s) 1061
BsuRI GGCC 1 cut(s) 360
BsuTUI ATCGAT 1 cut(s) 1061
BtgI CCRYGG 1 cut(s) 206
BtrI CACGTC 1 cut(s) 1045
BtsCI GGATG 2 cut(s) 698, 928
BtsIMutI CAGTG 1 cut(s) 1280
CfoI GCGC 2 cut(s) 1192, 1288
Cfr10I RCCGGY 7 cut(s) 399, 471, 612, 652, 681, 1186, 1246
Cfr13I GGNCC 1 cut(s) 1051
ClaI ATCGAT 1 cut(s) 1061
CseI GACGC 1 cut(s) 599
Csp6I GTAC 5 cut(s) 94, 127, 146, 411, 538
CspAI ACCGGT 5 cut(s) 399, 471, 612, 652, 681
CspCI CAANNNNNGTGG 2 cut(s) 1257, 1292
CviAII CATG 5 cut(s) 193, 640, 784, 901, 1239
CviQI GTAC 5 cut(s) 94, 127, 146, 411, 538
DdeI CTNAG 3 cut(s) 461, 849, 947
DinI GGCGCC 2 cut(s) 1191, 1287
DpnI GATC 8 cut(s) 197, 235, 315, 459, 531, 585, 740, 1060
DpnII GATC 8 cut(s) 195, 233, 313, 457, 529, 583, 738, 1058
Eam1104I CTCTTC 2 cut(s) 345, 1135
EarI CTCTTC 2 cut(s) 345, 1135
EciI GGCGGA 2 cut(s) 49, 1233
Eco32I GATATC 1 cut(s) 331
Eco47I GGWCC 1 cut(s) 1051
Eco57I CTGAAG 1 cut(s) 369
Eco72I CACGTG 2 cut(s) 239, 996
Eco88I CYCGRG 1 cut(s) 99
EcoRII CCWGG 3 cut(s) 70, 321, 1123
EcoRV GATATC 1 cut(s) 331
EgeI GGCGCC 2 cut(s) 1191, 1287
EheI GGCGCC 2 cut(s) 1191, 1287
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 5 cut(s) 196, 643, 787, 904, 1242
FaqI GGGAC 1 cut(s) 1064
FatI CATG 5 cut(s) 192, 639, 783, 900, 1238
FblI GTMKAC 3 cut(s) 697, 772, 1251
FokI GGATG 2 cut(s) 705, 935
FspBI CTAG 4 cut(s) 665, 671, 1007, 1233
GlaI GCGC 2 cut(s) 1191, 1287
HaeII RGCGCY 2 cut(s) 1193, 1289
HaeIII GGCC 1 cut(s) 360
HgaI GACGC 1 cut(s) 599
HhaI GCGC 2 cut(s) 1192, 1288
Hin1I GRCGYC 2 cut(s) 1190, 1286
Hin1II CATG 5 cut(s) 196, 643, 787, 904, 1242
Hin6I GCGC 2 cut(s) 1190, 1286
HinP1I GCGC 2 cut(s) 1190, 1286
HincII GTYRAC 3 cut(s) 391, 637, 1218
HindII GTYRAC 3 cut(s) 391, 637, 1218
HinfI GANTC 8 cut(s) 211, 272, 439, 566, 727, 871, 1106, 1153
HpaI GTTAAC 2 cut(s) 391, 637
HphI GGTGA 7 cut(s) 25, 566, 642, 970, 1155, 1197, 1248
Hpy188I TCNGA 6 cut(s) 202, 216, 271, 839, 864, 1152
Hpy188III TCNNGA 6 cut(s) 278, 527, 587, 601, 875, 1007
Hpy99I CGWCG 3 cut(s) 44, 68, 284
HpyAV CCTTC 2 cut(s) 545, 683
HpyCH4III ACNGT 5 cut(s) 40, 257, 301, 758, 808
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 176, 934, 943
HpyF3I CTNAG 3 cut(s) 461, 849, 947
Hsp92I GRCGYC 2 cut(s) 1190, 1286
Hsp92II CATG 5 cut(s) 196, 643, 787, 904, 1242
HspAI GCGC 2 cut(s) 1190, 1286
KasI GGCGCC 2 cut(s) 1189, 1285
KspAI GTTAAC 2 cut(s) 391, 637
Kzo9I GATC 8 cut(s) 195, 233, 313, 457, 529, 583, 738, 1058
LguI GCTCTTC 2 cut(s) 345, 1135
LmnI GCTCC 3 cut(s) 73, 83, 272
MaeI CTAG 4 cut(s) 665, 671, 1007, 1233
MaeIII GTNAC 7 cut(s) 171, 608, 802, 843, 896, 1103, 1278
MalI GATC 8 cut(s) 197, 235, 315, 459, 531, 585, 740, 1060
MboI GATC 8 cut(s) 195, 233, 313, 457, 529, 583, 738, 1058
MboII GAAGA 3 cut(s) 362, 1122, 1161
MflI RGATCY 1 cut(s) 583
MluCI AATT 7 cut(s) 485, 515, 557, 646, 1012, 1018, 1030
Mly113I GGCGCC 2 cut(s) 1190, 1286
MlyI GAGTC 4 cut(s) 448, 721, 1100, 1162
MmeI TCCRAC 1 cut(s) 817
MnlI CCTC 9 cut(s) 132, 371, 685, 871, 1041, 1155, 1158, 1236, 1272
MroXI GAANNNNTTC 1 cut(s) 1034
MseI TTAA 7 cut(s) 26, 294, 365, 390, 518, 636, 914
MslI CAYNNNNRTG 2 cut(s) 241, 746
MspR9I CCNGG 7 cut(s) 72, 82, 323, 419, 491, 632, 1125
MvaI CCWGG 3 cut(s) 72, 323, 1125
MwoI GCNNNNNNNGC 4 cut(s) 167, 176, 934, 943
NarI GGCGCC 2 cut(s) 1190, 1286
NciI CCSGG 4 cut(s) 82, 419, 491, 632
NdeII GATC 8 cut(s) 195, 233, 313, 457, 529, 583, 738, 1058
NlaIII CATG 5 cut(s) 196, 643, 787, 904, 1242
NlaIV GGNNCC 5 cut(s) 79, 1052, 1185, 1191, 1287
NmeAIII GCCGAG 1 cut(s) 729
NmuCI GTSAC 2 cut(s) 1103, 1278
NspI RCATGY 1 cut(s) 904
NspV TTCGAA 2 cut(s) 138, 450
OliI CACNNNNGTG 1 cut(s) 241
PciSI GCTCTTC 2 cut(s) 345, 1135
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PdmI GAANNNNTTC 1 cut(s) 1034
PfeI GAWTC 4 cut(s) 211, 272, 566, 871
Pfl23II CGTACG 1 cut(s) 145
PflFI GACNNNGTC 1 cut(s) 1239
PfoI TCCNGGA 1 cut(s) 489
PinAI ACCGGT 5 cut(s) 399, 471, 612, 652, 681
PleI GAGTC 4 cut(s) 447, 721, 1100, 1161
PluTI GGCGCC 2 cut(s) 1193, 1289
PmaCI CACGTG 2 cut(s) 239, 996
PmlI CACGTG 2 cut(s) 239, 996
PpsI GAGTC 4 cut(s) 447, 721, 1100, 1161
Ppu21I YACGTR 3 cut(s) 166, 239, 996
PshBI ATTAAT 1 cut(s) 26
PsiI TTATAA 2 cut(s) 825, 1017
Psp1406I AACGTT 2 cut(s) 801, 888
Psp6I CCWGG 3 cut(s) 70, 321, 1123
PspCI CACGTG 2 cut(s) 239, 996
PspGI CCWGG 3 cut(s) 70, 321, 1123
PspLI CGTACG 1 cut(s) 145
PspN4I GGNNCC 5 cut(s) 79, 1052, 1185, 1191, 1287
PspPI GGNCC 1 cut(s) 1051
PsuI RGATCY 1 cut(s) 583
PsyI GACNNNGTC 1 cut(s) 1239
RsaI GTAC 5 cut(s) 95, 128, 147, 412, 539
RsaNI GTAC 5 cut(s) 94, 127, 146, 411, 538
RseI CAYNNNNRTG 2 cut(s) 241, 746
SapI GCTCTTC 2 cut(s) 345, 1135
SaqAI TTAA 7 cut(s) 26, 294, 365, 390, 518, 636, 914
Sau3AI GATC 8 cut(s) 195, 233, 313, 457, 529, 583, 738, 1058
Sau96I GGNCC 1 cut(s) 1051
SchI GAGTC 4 cut(s) 448, 721, 1100, 1162
ScrFI CCNGG 7 cut(s) 72, 82, 323, 419, 491, 632, 1125
SfcI CTRYAG 1 cut(s) 1095
SfoI GGCGCC 2 cut(s) 1191, 1287
SfuI TTCGAA 2 cut(s) 138, 450
SgrAI CRCCGGYG 1 cut(s) 1186
SinI GGWCC 1 cut(s) 1051
SmiMI CAYNNNNRTG 2 cut(s) 241, 746
SmlI CTYRAG 2 cut(s) 875, 938
SmoI CTYRAG 2 cut(s) 875, 938
Sse9I AATT 7 cut(s) 485, 515, 557, 646, 1012, 1018, 1030
SsiI CCGC 4 cut(s) 60, 701, 1223, 1244
SspDI GGCGCC 2 cut(s) 1189, 1285
SspI AATATT 2 cut(s) 30, 135
SspMI CTAG 4 cut(s) 665, 671, 1007, 1233
StyD4I CCNGG 7 cut(s) 70, 80, 321, 417, 489, 630, 1123
TaaI ACNGT 5 cut(s) 40, 257, 301, 758, 808
TaqI TCGA 8 cut(s) 138, 153, 279, 450, 576, 854, 1061, 1088
TasI AATT 7 cut(s) 485, 515, 557, 646, 1012, 1018, 1030
TatI WGTACW 1 cut(s) 93
TfiI GAWTC 4 cut(s) 211, 272, 566, 871
Tru1I TTAA 7 cut(s) 26, 294, 365, 390, 518, 636, 914
Tru9I TTAA 7 cut(s) 26, 294, 365, 390, 518, 636, 914
TscAI CASTG 1 cut(s) 1287
TseFI GTSAC 2 cut(s) 1103, 1278
Tsp45I GTSAC 2 cut(s) 1103, 1278
TspDTI ATGAA 1 cut(s) 1043
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 1 cut(s) 1287
Tth111I GACNNNGTC 1 cut(s) 1239
VpaK11BI GGWCC 1 cut(s) 1051
VspI ATTAAT 1 cut(s) 26
XapI RAATTY 2 cut(s) 485, 646
XbaI TCTAGA 1 cut(s) 1006
XceI RCATGY 1 cut(s) 904
XmiI GTMKAC 3 cut(s) 697, 772, 1251
XmnI GAANNNNTTC 1 cut(s) 1034
XspI CTAG 4 cut(s) 665, 671, 1007, 1233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.