Rw6G044750

leucine-rich repeat extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012723
Physical Location & Seq
Forward (+)
67889116 .. 67889947
832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G044750.1

Sequence Viewer

Length: 738 bp
ATGTCACCCTCGCTTATTTTTCCCAACCCGAGAATCAAGACTGCCTACATTGCCCTCCAAGCATGGAAGCAAGCCATGCTCTCAGACCCTCTGGGACACACAGCCAACTGGGTTGGATCCAATGTCTGCAACTACACTGGAGTCTTCTGTGCTGAAGCTCTCGACGACCCAACAATAGAAACAGTCGCTGGCATCGATCTAAACCATGCTGACATTGCCGGGTACCTCCCAGAAGAGCTTGGCCATCTTTCAGACATTGCATTGTTTCACGTGAACTCCAATCGCTTCTGTGGCACTGTCCCAAAGAGTTTCAGCAAGTTGAAGCTTCTTCATGAGCTTGATCTAAGTAATAACCGGTTTGCTGGGAAATTCCCTCTTGTTGTTCTTCAACTGCCAGTACTTAAATACCTTGACATTCGGTTCAATGAATTTGAAGGTAGAATTCCCAAGGAACTATTTGATAAGCATCTTGATGCCATCTTCCTCAACGACAACAGATTCACCTATGAACTGCCACATAATTTAGGAAACTCGCCGGTCTCAGTTATTGTTCTTGCAAACAATAAGTTCCACGGATGTTTACCGGCCAGCCTTGGCAACATGTCCAAGAACTTAAACGAGCTGATCCTTACTAGAAATGGGTTGTACTCCTGTTTGCCAGAGGAAATAGGAATGCTCAAGAATCTTACTGTGTTAGATGGACTATACCAGAGAGCATTTGTGGGCTTCCAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005199
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8

Protein Analysis

245

Amino Acids

27.33

Weight (kDa)

6.05

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 18 - 50 8.8e-07 Leucine rich repeat N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012723)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25550
fragaria_vesca FvH4_2g30240
malus_domestica MD08G1135200.v1.1 MD15G1114000.v1.1
prunus_persica Prupe.1G465200_v2.0.a1
pyrus_communis pycom08g11410 pycom15g10230
rosa_chinensis RchiOBHm_Chr6g0312631
rosa_laevigata RLG00000010309
rosa_multiflora Rmu_sc0002859.1_g000007 Rmu_sc0034244.1_g000001 Rmu_ssc0000412.1_g000003
rosa_roxburghii Rroxscaffold_7G00156630
rosa_rugosa Rorug06G0398000
rosa_samantha Rh6AG515300 Rh6BG524800 Rh6CG530200
rosa_wichuraiana Rw6G044750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 222
AccB1I GGYRCC 1 cut(s) 222
AclWI GGATC 3 cut(s) 111, 124, 619
AcoI YGGCCR 2 cut(s) 241, 585
AcsI RAATTY 3 cut(s) 368, 428, 441
AcuI CTGAAG 1 cut(s) 174
AcvI CACGTG 1 cut(s) 271
AfaI GTAC 3 cut(s) 224, 399, 647
AflIII ACRYGT 1 cut(s) 600
AgeI ACCGGT 1 cut(s) 354
AgsI TTSAA 4 cut(s) 322, 389, 424, 434
AluBI AGCT 5 cut(s) 158, 238, 325, 337, 622
AluI AGCT 5 cut(s) 158, 238, 325, 337, 622
Alw26I GTCTC 1 cut(s) 544
AlwI GGATC 3 cut(s) 111, 124, 619
AlwNI CAGNNNCTG 1 cut(s) 188
Ama87I CYCGRG 1 cut(s) 28
AoxI GGCC 2 cut(s) 241, 585
ApoI RAATTY 3 cut(s) 368, 428, 441
AsiGI ACCGGT 1 cut(s) 354
Asp718I GGTACC 1 cut(s) 222
AsuC2I CCSGG 1 cut(s) 220
AsuHPI GGTGA 1 cut(s) 493
AvaI CYCGRG 1 cut(s) 28
BalI TGGCCA 1 cut(s) 243
BamHI GGATCC 1 cut(s) 116
BanI GGYRCC 1 cut(s) 222
BbrPI CACGTG 1 cut(s) 271
BbsI GAAGAC 1 cut(s) 136
BccI CCATC 3 cut(s) 252, 485, 692
BcnI CCSGG 1 cut(s) 220
BcoDI GTCTC 1 cut(s) 544
BfaI CTAG 1 cut(s) 633
BmcAI AGTACT 1 cut(s) 399
Bme1390I CCNGG 1 cut(s) 220
BmeT110I CYCGRG 1 cut(s) 28
BmiI GGNNCC 2 cut(s) 118, 224
BmrFI CCNGG 1 cut(s) 220
BmrI ACTGGG 1 cut(s) 118
BmsI GCATC 3 cut(s) 201, 463, 475
BmuI ACTGGG 1 cut(s) 118
BpiI GAAGAC 1 cut(s) 136
BpmI CTGGAG 1 cut(s) 159
BpuEI CTTGAG 1 cut(s) 662
BpuMI CCSGG 1 cut(s) 220
Bsa29I ATCGAT 1 cut(s) 195
BsaAI YACGTR 1 cut(s) 271
BsaBI GATNNNNATC 1 cut(s) 465
BsaI GGTCTC 1 cut(s) 544
BsaJI CCNNGG 3 cut(s) 447, 571, 592
BsaWI WCCGGW 1 cut(s) 354
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bse118I RCCGGY 3 cut(s) 354, 535, 583
Bse1I ACTGG 3 cut(s) 113, 142, 395
Bse3DI GCAATG 3 cut(s) 48, 213, 255
Bse8I GATNNNNATC 1 cut(s) 465
BseCI ATCGAT 1 cut(s) 195
BseDI CCNNGG 3 cut(s) 447, 571, 592
BseGI GGATG 1 cut(s) 581
BseJI GATNNNNATC 1 cut(s) 465
BseMI GCAATG 3 cut(s) 48, 213, 255
BseMII CTCAG 2 cut(s) 96, 555
BseNI ACTGG 3 cut(s) 113, 142, 395
BseYI CCCAGC 1 cut(s) 362
BshFI GGCC 2 cut(s) 243, 587
BshNI GGYRCC 1 cut(s) 222
BshTI ACCGGT 1 cut(s) 354
BshVI ATCGAT 1 cut(s) 195
BsiHKCI CYCGRG 1 cut(s) 28
BsiSI CCGG 4 cut(s) 219, 355, 536, 584
BslFI GGGAC 2 cut(s) 108, 284
BsmAI GTCTC 1 cut(s) 544
BsmFI GGGAC 2 cut(s) 108, 284
BsmI GAATGC 1 cut(s) 678
BsnI GGCC 2 cut(s) 243, 587
Bso31I GGTCTC 1 cut(s) 544
BsoBI CYCGRG 1 cut(s) 28
Bsp143I GATC 4 cut(s) 116, 196, 340, 624
BspANI GGCC 2 cut(s) 243, 587
BspCNI CTCAG 2 cut(s) 95, 554
BspDI ATCGAT 1 cut(s) 195
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 2 cut(s) 118, 224
BspPI GGATC 3 cut(s) 111, 124, 619
BspQI GCTCTTC 1 cut(s) 228
BspT107I GGYRCC 1 cut(s) 222
BspTNI GGTCTC 1 cut(s) 544
BsrDI GCAATG 3 cut(s) 48, 213, 255
BsrFI RCCGGY 3 cut(s) 354, 535, 583
BsrI ACTGG 3 cut(s) 113, 142, 395
BssAI RCCGGY 3 cut(s) 354, 535, 583
BssECI CCNNGG 3 cut(s) 447, 571, 592
BssMI GATC 4 cut(s) 116, 196, 340, 624
BssT1I CCWWGG 2 cut(s) 447, 592
Bst4CI ACNGT 3 cut(s) 184, 298, 691
Bst6I CTCTTC 1 cut(s) 228
BstAPI GCANNNNNTGC 1 cut(s) 76
BstBAI YACGTR 1 cut(s) 271
BstC8I GCNNGC 3 cut(s) 72, 190, 589
BstDEI CTNAG 3 cut(s) 82, 344, 541
BstDSI CCRYGG 1 cut(s) 571
BstF5I GGATG 1 cut(s) 581
BstKTI GATC 4 cut(s) 119, 199, 343, 627
BstMAI GTCTC 1 cut(s) 544
BstMBI GATC 4 cut(s) 116, 196, 340, 624
BstMWI GCNNNNNNNGC 5 cut(s) 50, 59, 76, 215, 291
BstNSI RCATGY 1 cut(s) 604
BstSCI CCNGG 1 cut(s) 218
BstV2I GAAGAC 1 cut(s) 136
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
Bsu15I ATCGAT 1 cut(s) 195
BsuRI GGCC 2 cut(s) 243, 587
BsuTUI ATCGAT 1 cut(s) 195
BtgI CCRYGG 1 cut(s) 571
BtsCI GGATG 1 cut(s) 581
BtsIMutI CAGTG 2 cut(s) 135, 294
Cac8I GCNNGC 3 cut(s) 72, 190, 589
CaiI CAGNNNCTG 1 cut(s) 188
CciI TCATGA 1 cut(s) 331
Cfr10I RCCGGY 3 cut(s) 354, 535, 583
ClaI ATCGAT 1 cut(s) 195
Csp6I GTAC 3 cut(s) 223, 398, 646
CspAI ACCGGT 1 cut(s) 354
CviAII CATG 5 cut(s) 63, 76, 206, 332, 601
CviQI GTAC 3 cut(s) 223, 398, 646
DdeI CTNAG 3 cut(s) 82, 344, 541
DpnI GATC 4 cut(s) 118, 198, 342, 626
DpnII GATC 4 cut(s) 116, 196, 340, 624
EaeI YGGCCR 2 cut(s) 241, 585
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
Eco130I CCWWGG 2 cut(s) 447, 592
Eco31I GGTCTC 1 cut(s) 544
Eco57I CTGAAG 1 cut(s) 174
Eco72I CACGTG 1 cut(s) 271
Eco88I CYCGRG 1 cut(s) 28
EcoRI GAATTC 1 cut(s) 441
EcoT14I CCWWGG 2 cut(s) 447, 592
ErhI CCWWGG 2 cut(s) 447, 592
FaeI CATG 5 cut(s) 66, 79, 209, 335, 604
FaiI YATR 8 cut(s) 64, 77, 207, 333, 507, 519, 602, 706
FaqI GGGAC 2 cut(s) 108, 284
FatI CATG 5 cut(s) 62, 75, 205, 331, 600
FokI GGATG 1 cut(s) 588
FspBI CTAG 1 cut(s) 633
GsaI CCCAGC 1 cut(s) 366
GsuI CTGGAG 1 cut(s) 159
HaeIII GGCC 2 cut(s) 243, 587
HapII CCGG 4 cut(s) 219, 355, 536, 584
Hin1II CATG 5 cut(s) 66, 79, 209, 335, 604
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 4 cut(s) 33, 141, 498, 682
HpaII CCGG 4 cut(s) 219, 355, 536, 584
HphI GGTGA 1 cut(s) 493
Hpy166II GTNNAC 2 cut(s) 274, 581
Hpy188I TCNGA 3 cut(s) 85, 253, 737
Hpy188III TCNNGA 5 cut(s) 37, 161, 332, 470, 679
Hpy8I GTNNAC 2 cut(s) 274, 581
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 1 cut(s) 428
HpyCH4III ACNGT 3 cut(s) 184, 298, 691
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 3 cut(s) 129, 260, 557
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 59, 76, 215, 291
HpyF3I CTNAG 3 cut(s) 82, 344, 541
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 5 cut(s) 66, 79, 209, 335, 604
KpnI GGTACC 1 cut(s) 226
Kzo9I GATC 4 cut(s) 116, 196, 340, 624
LguI GCTCTTC 1 cut(s) 228
LweI GCATC 3 cut(s) 201, 463, 475
MaeI CTAG 1 cut(s) 633
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 1 cut(s) 3
MalI GATC 4 cut(s) 118, 198, 342, 626
MboI GATC 4 cut(s) 116, 196, 340, 624
MboII GAAGA 5 cut(s) 136, 245, 320, 377, 472
MflI RGATCY 1 cut(s) 116
MlsI TGGCCA 1 cut(s) 243
MluCI AATT 4 cut(s) 368, 428, 441, 520
MluNI TGGCCA 1 cut(s) 243
MlyI GAGTC 1 cut(s) 150
MmeI TCCRAC 1 cut(s) 94
MnlI CCTC 7 cut(s) 19, 65, 99, 236, 384, 494, 655
Mox20I TGGCCA 1 cut(s) 243
MscI TGGCCA 1 cut(s) 243
MseI TTAA 2 cut(s) 402, 614
MslI CAYNNNNRTG 1 cut(s) 471
Msp20I TGGCCA 1 cut(s) 243
MspI CCGG 4 cut(s) 219, 355, 536, 584
MspR9I CCNGG 1 cut(s) 220
Mva1269I GAATGC 1 cut(s) 678
MwoI GCNNNNNNNGC 5 cut(s) 50, 59, 76, 215, 291
NciI CCSGG 1 cut(s) 220
NdeII GATC 4 cut(s) 116, 196, 340, 624
NlaIII CATG 5 cut(s) 66, 79, 209, 335, 604
NlaIV GGNNCC 2 cut(s) 118, 224
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 1 cut(s) 604
PagI TCATGA 1 cut(s) 331
PciI ACATGT 1 cut(s) 600
PciSI GCTCTTC 1 cut(s) 228
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PctI GAATGC 1 cut(s) 678
PfeI GAWTC 3 cut(s) 33, 498, 682
PinAI ACCGGT 1 cut(s) 354
PleI GAGTC 1 cut(s) 149
PmaCI CACGTG 1 cut(s) 271
PmlI CACGTG 1 cut(s) 271
PpsI GAGTC 1 cut(s) 149
Ppu21I YACGTR 1 cut(s) 271
PscI ACATGT 1 cut(s) 600
PspCI CACGTG 1 cut(s) 271
PspFI CCCAGC 1 cut(s) 362
PspN4I GGNNCC 2 cut(s) 118, 224
PstNI CAGNNNCTG 1 cut(s) 188
PsuI RGATCY 1 cut(s) 116
RsaI GTAC 3 cut(s) 224, 399, 647
RsaNI GTAC 3 cut(s) 223, 398, 646
RseI CAYNNNNRTG 1 cut(s) 471
SapI GCTCTTC 1 cut(s) 228
SaqAI TTAA 2 cut(s) 402, 614
Sau3AI GATC 4 cut(s) 116, 196, 340, 624
ScaI AGTACT 1 cut(s) 399
SchI GAGTC 1 cut(s) 150
ScrFI CCNGG 1 cut(s) 220
SfaNI GCATC 3 cut(s) 201, 463, 475
SmiMI CAYNNNNRTG 1 cut(s) 471
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
Sse9I AATT 4 cut(s) 368, 428, 441, 520
SspMI CTAG 1 cut(s) 633
StyD4I CCNGG 1 cut(s) 218
StyI CCWWGG 2 cut(s) 447, 592
TaaI ACNGT 3 cut(s) 184, 298, 691
TaiI ACGT 1 cut(s) 273
TaqI TCGA 2 cut(s) 162, 195
TasI AATT 4 cut(s) 368, 428, 441, 520
TatI WGTACW 2 cut(s) 397, 645
TfiI GAWTC 3 cut(s) 33, 498, 682
Tru1I TTAA 2 cut(s) 402, 614
Tru9I TTAA 2 cut(s) 402, 614
TscAI CASTG 2 cut(s) 142, 301
TseFI GTSAC 1 cut(s) 3
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 3 cut(s) 320, 441, 522
TspGWI ACGGA 1 cut(s) 588
TspRI CASTG 2 cut(s) 142, 301
XapI RAATTY 3 cut(s) 368, 428, 441
XceI RCATGY 1 cut(s) 604
XspI CTAG 1 cut(s) 633
ZrmI AGTACT 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.