RchiOBHm_Chr6g0312631

leucine-rich repeat extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0012723
Physical Location & Seq
Forward (+)
69234675 .. 69236862
2188 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28125

Sequence Viewer

Length: 1107 bp
ATGAAGAGAAAGATTGATCACACTCGCACTATTCTCATACTCTTCTTTTGCCTCAGCACTACTACTACTGCCCAAGAACCCCATATTTTCCACAATGCAGGCTTAACAGACTCCCACATCAAGCAAAGGCTGCTCCTTAATGACGGTTCCAGAGGTGAGACTGTCACGGTGTCACCCTCACTTATTTTTCCCAACCCGAGAATCAAGACTGCCTACGTTGCCCTCCAAGCATGGAAGCAAGCCATGCTCTCAGACCCTCTGGGACACACAGCCAACTGGATTGGATCCAATGTCTGCAACTACACTGGAGTCTTCTGTGCTGAAGCTCTCGACGACCCAACAATAGAAACAGTCGCTGGCATCGATCTAAACCATGCTGACATTGCCGGGTACCTCCCAGAAGAGCTTGGCCATCTTTCAGACATTGCATTGTTTCACGTGAACTCCAATCGTTTCTGTGGCACTGTCCCAAAGAGTTTCAGCAAGTTGAAGCTTCTTCATGAGCTTGATCTAAGTAATAACCGGTTTGCTGGGAAATTCCCTCTTGTTGTTCTTCAACTGCCAGTACTTAAATACCTTGACATTCGGTTCAATGAATTTGAAGGTAGAATTCCCAAGGACCTATTTGATAAGCATCTTGATGCCATCTTCCTCAACGACAACAGATTCGCCTATGAACTGCCACATAATTTAGGAAACTCGCCGGTCTCAGTTATTGTTCTTGCAAACAATAAGTTCCATGGATGTTTACCGGCCAGCCTTGGCAACATGTCCAAGAACTTAAACGAGCTGATCCTTACTAGAAATGGGTTGTACTCCTGTTTGCCAGAGGAAATAGGAATGCTCAAGAATCTTACCGTGTTAGATGTGAGTCATAATGGTCTTTTCGGTGAGTTGCCACACGCACTAGGTGAAATGTTGAGCTTGGAGGAGCTGAATGTGGCTCATAACAATCTTACAGGGACTATACCAGAGAGCATTTGTGGGCTTCCAAATCTGAAAAAATTTAGCTTTGGGTACAACTTTTTCACAGGTGAGCCACCAGTGTGTTTAGATTTGGAAGAGTTTGATGATAGCAGGAATTGTTTGCGGCTCTGTTGGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005199
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8

Protein Analysis

368

Amino Acids

41.04

Weight (kDa)

5.82

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 74 - 106 4.8e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 272 - 336 3.3e-06 Leucine-rich repeat region
LRR_8 PF13855 284 - 337 5.4e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012723)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25550
fragaria_vesca FvH4_2g30240
malus_domestica MD08G1135200.v1.1 MD15G1114000.v1.1
prunus_persica Prupe.1G465200_v2.0.a1
pyrus_communis pycom08g11410 pycom15g10230
rosa_chinensis RchiOBHm_Chr6g0312631
rosa_laevigata RLG00000010309
rosa_multiflora Rmu_sc0002859.1_g000007 Rmu_sc0034244.1_g000001 Rmu_ssc0000412.1_g000003
rosa_roxburghii Rroxscaffold_7G00156630
rosa_rugosa Rorug06G0398000
rosa_samantha Rh6AG515300 Rh6BG524800 Rh6CG530200
rosa_wichuraiana Rw6G044750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 390
AccB1I GGYRCC 1 cut(s) 390
AciI CCGC 1 cut(s) 1090
AclWI GGATC 3 cut(s) 279, 292, 787
AcoI YGGCCR 2 cut(s) 409, 753
AcsI RAATTY 4 cut(s) 536, 596, 609, 1004
AcuI CTGAAG 1 cut(s) 342
AcvI CACGTG 1 cut(s) 439
AdeI CACNNNGTG 1 cut(s) 911
AfaI GTAC 4 cut(s) 392, 567, 815, 1019
AflIII ACRYGT 1 cut(s) 768
AgeI ACCGGT 1 cut(s) 522
AgsI TTSAA 4 cut(s) 490, 557, 592, 602
AleI CACNNNNGTG 1 cut(s) 1045
AluBI AGCT 8 cut(s) 326, 406, 493, 505, 790, 924, 934, 1011
AluI AGCT 8 cut(s) 326, 406, 493, 505, 790, 924, 934, 1011
Alw26I GTCTC 2 cut(s) 152, 712
AlwI GGATC 3 cut(s) 279, 292, 787
AlwNI CAGNNNCTG 1 cut(s) 356
Ama87I CYCGRG 1 cut(s) 196
AoxI GGCC 2 cut(s) 409, 753
ApeKI GCWGC 1 cut(s) 130
ApoI RAATTY 4 cut(s) 536, 596, 609, 1004
ArsI GACNNNNNNTTYG 2 cut(s) 650, 682
AsiGI ACCGGT 1 cut(s) 522
Asp718I GGTACC 1 cut(s) 390
AspS9I GGNCC 1 cut(s) 619
AsuC2I CCSGG 1 cut(s) 388
AsuHPI GGTGA 5 cut(s) 165, 167, 902, 923, 1046
AvaI CYCGRG 1 cut(s) 196
AvaII GGWCC 1 cut(s) 619
BalI TGGCCA 1 cut(s) 411
BamHI GGATCC 1 cut(s) 284
BanI GGYRCC 1 cut(s) 390
BbrPI CACGTG 1 cut(s) 439
BbsI GAAGAC 1 cut(s) 304
BbvCI CCTCAGC 1 cut(s) 53
BbvI GCAGC 1 cut(s) 117
BccI CCATC 2 cut(s) 420, 653
BclI TGATCA 1 cut(s) 16
BcnI CCSGG 1 cut(s) 388
BcoDI GTCTC 2 cut(s) 152, 712
BfaI CTAG 2 cut(s) 801, 908
BisI GCNGC 2 cut(s) 131, 1091
BlsI GCNGC 2 cut(s) 132, 1092
BmcAI AGTACT 1 cut(s) 567
Bme1390I CCNGG 1 cut(s) 388
Bme18I GGWCC 1 cut(s) 619
BmeT110I CYCGRG 1 cut(s) 196
BmgT120I GGNCC 1 cut(s) 619
BmiI GGNNCC 3 cut(s) 148, 286, 392
BmrFI CCNGG 1 cut(s) 388
BmsI GCATC 3 cut(s) 369, 631, 643
BpiI GAAGAC 1 cut(s) 304
BpmI CTGGAG 1 cut(s) 327
Bpu10I CCTNAGC 1 cut(s) 53
BpuEI CTTGAG 1 cut(s) 830
BpuMI CCSGG 1 cut(s) 388
Bsa29I ATCGAT 1 cut(s) 363
BsaAI YACGTR 1 cut(s) 439
BsaBI GATNNNNATC 1 cut(s) 633
BsaI GGTCTC 1 cut(s) 712
BsaJI CCNNGG 3 cut(s) 615, 739, 760
BsaWI WCCGGW 1 cut(s) 522
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bse118I RCCGGY 3 cut(s) 522, 703, 751
Bse1I ACTGG 4 cut(s) 281, 310, 563, 1043
Bse3DI GCAATG 2 cut(s) 381, 423
Bse8I GATNNNNATC 1 cut(s) 633
BseCI ATCGAT 1 cut(s) 363
BseDI CCNNGG 3 cut(s) 615, 739, 760
BseGI GGATG 1 cut(s) 749
BseJI GATNNNNATC 1 cut(s) 633
BseMI GCAATG 2 cut(s) 381, 423
BseMII CTCAG 3 cut(s) 67, 264, 723
BseNI ACTGG 4 cut(s) 281, 310, 563, 1043
BseRI GAGGAG 1 cut(s) 944
BseXI GCAGC 1 cut(s) 117
BseYI CCCAGC 1 cut(s) 530
BshFI GGCC 2 cut(s) 411, 755
BshNI GGYRCC 1 cut(s) 390
BshTI ACCGGT 1 cut(s) 522
BshVI ATCGAT 1 cut(s) 363
BsiHKCI CYCGRG 1 cut(s) 196
BsiSI CCGG 4 cut(s) 387, 523, 704, 752
BslFI GGGAC 3 cut(s) 276, 452, 976
BsmAI GTCTC 2 cut(s) 152, 712
BsmFI GGGAC 3 cut(s) 276, 452, 976
BsmI GAATGC 1 cut(s) 846
BsnI GGCC 2 cut(s) 411, 755
Bso31I GGTCTC 1 cut(s) 712
BsoBI CYCGRG 1 cut(s) 196
Bsp143I GATC 5 cut(s) 16, 284, 364, 508, 792
Bsp19I CCATGG 1 cut(s) 739
BspACI CCGC 1 cut(s) 1090
BspANI GGCC 2 cut(s) 411, 755
BspCNI CTCAG 3 cut(s) 66, 263, 722
BspDI ATCGAT 1 cut(s) 363
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 3 cut(s) 148, 286, 392
BspPI GGATC 3 cut(s) 279, 292, 787
BspQI GCTCTTC 1 cut(s) 396
BspT107I GGYRCC 1 cut(s) 390
BspTNI GGTCTC 1 cut(s) 712
BsrDI GCAATG 2 cut(s) 381, 423
BsrFI RCCGGY 3 cut(s) 522, 703, 751
BsrI ACTGG 4 cut(s) 281, 310, 563, 1043
BssAI RCCGGY 3 cut(s) 522, 703, 751
BssECI CCNNGG 3 cut(s) 615, 739, 760
BssMI GATC 5 cut(s) 16, 284, 364, 508, 792
BssT1I CCWWGG 3 cut(s) 615, 739, 760
Bst4CI ACNGT 6 cut(s) 146, 163, 169, 352, 466, 859
Bst6I CTCTTC 3 cut(s) 47, 396, 1056
BstAPI GCANNNNNTGC 2 cut(s) 130, 244
BstBAI YACGTR 1 cut(s) 439
BstC8I GCNNGC 4 cut(s) 100, 240, 358, 757
BstDEI CTNAG 4 cut(s) 53, 250, 512, 709
BstDSI CCRYGG 1 cut(s) 739
BstF5I GGATG 1 cut(s) 749
BstKTI GATC 5 cut(s) 19, 287, 367, 511, 795
BstMAI GTCTC 2 cut(s) 152, 712
BstMBI GATC 5 cut(s) 16, 284, 364, 508, 792
BstMWI GCNNNNNNNGC 5 cut(s) 130, 218, 227, 244, 383
BstNSI RCATGY 1 cut(s) 772
BstSCI CCNGG 1 cut(s) 386
BstV1I GCAGC 1 cut(s) 117
BstV2I GAAGAC 1 cut(s) 304
BstX2I RGATCY 1 cut(s) 284
BstYI RGATCY 1 cut(s) 284
Bsu15I ATCGAT 1 cut(s) 363
BsuRI GGCC 2 cut(s) 411, 755
BsuTUI ATCGAT 1 cut(s) 363
BtgI CCRYGG 1 cut(s) 739
BtsCI GGATG 1 cut(s) 749
BtsIMutI CAGTG 3 cut(s) 303, 462, 1050
Cac8I GCNNGC 4 cut(s) 100, 240, 358, 757
CaiI CAGNNNCTG 1 cut(s) 356
CciI TCATGA 1 cut(s) 499
Cfr10I RCCGGY 3 cut(s) 522, 703, 751
Cfr13I GGNCC 1 cut(s) 619
ClaI ATCGAT 1 cut(s) 363
Csp6I GTAC 4 cut(s) 391, 566, 814, 1018
CspAI ACCGGT 1 cut(s) 522
CviAII CATG 6 cut(s) 231, 244, 374, 500, 740, 769
CviQI GTAC 4 cut(s) 391, 566, 814, 1018
DdeI CTNAG 4 cut(s) 53, 250, 512, 709
DpnI GATC 5 cut(s) 18, 286, 366, 510, 794
DpnII GATC 5 cut(s) 16, 284, 364, 508, 792
DraIII CACNNNGTG 1 cut(s) 911
EaeI YGGCCR 2 cut(s) 409, 753
Eam1104I CTCTTC 3 cut(s) 47, 396, 1056
EarI CTCTTC 3 cut(s) 47, 396, 1056
Eco130I CCWWGG 3 cut(s) 615, 739, 760
Eco31I GGTCTC 1 cut(s) 712
Eco47I GGWCC 1 cut(s) 619
Eco57I CTGAAG 1 cut(s) 342
Eco72I CACGTG 1 cut(s) 439
Eco88I CYCGRG 1 cut(s) 196
EcoO109I RGGNCCY 1 cut(s) 619
EcoRI GAATTC 1 cut(s) 609
EcoT14I CCWWGG 3 cut(s) 615, 739, 760
ErhI CCWWGG 3 cut(s) 615, 739, 760
FaeI CATG 6 cut(s) 234, 247, 377, 503, 743, 772
FaqI GGGAC 3 cut(s) 276, 452, 976
FatI CATG 6 cut(s) 230, 243, 373, 499, 739, 768
FbaI TGATCA 1 cut(s) 16
Fnu4HI GCNGC 2 cut(s) 131, 1091
FokI GGATG 1 cut(s) 756
Fsp4HI GCNGC 2 cut(s) 131, 1091
FspBI CTAG 2 cut(s) 801, 908
GluI GCNGC 2 cut(s) 131, 1091
GsaI CCCAGC 1 cut(s) 534
GsuI CTGGAG 1 cut(s) 327
HaeIII GGCC 2 cut(s) 411, 755
HapII CCGG 4 cut(s) 387, 523, 704, 752
Hin1II CATG 6 cut(s) 234, 247, 377, 503, 743, 772
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 6 cut(s) 110, 201, 309, 666, 850, 871
HpaII CCGG 4 cut(s) 387, 523, 704, 752
HphI GGTGA 5 cut(s) 165, 167, 902, 923, 1046
Hpy166II GTNNAC 2 cut(s) 442, 749
Hpy188I TCNGA 3 cut(s) 253, 421, 999
Hpy188III TCNNGA 6 cut(s) 150, 205, 329, 500, 638, 847
Hpy8I GTNNAC 2 cut(s) 442, 749
Hpy99I CGWCG 1 cut(s) 335
HpyAV CCTTC 1 cut(s) 596
HpyCH4III ACNGT 6 cut(s) 146, 163, 169, 352, 466, 859
HpyCH4IV ACGT 2 cut(s) 216, 438
HpyCH4V TGCA 4 cut(s) 98, 297, 428, 725
HpyF10VI GCNNNNNNNGC 5 cut(s) 130, 218, 227, 244, 383
HpyF3I CTNAG 4 cut(s) 53, 250, 512, 709
HpySE526I ACGT 2 cut(s) 216, 438
Hsp92II CATG 6 cut(s) 234, 247, 377, 503, 743, 772
KpnI GGTACC 1 cut(s) 394
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 5 cut(s) 16, 284, 364, 508, 792
LguI GCTCTTC 1 cut(s) 396
LmnI GCTCC 2 cut(s) 138, 931
Lsp1109I GCAGC 1 cut(s) 117
LweI GCATC 3 cut(s) 369, 631, 643
MaeI CTAG 2 cut(s) 801, 908
MaeII ACGT 2 cut(s) 216, 438
MaeIII GTNAC 2 cut(s) 163, 171
MalI GATC 5 cut(s) 18, 286, 366, 510, 794
MboI GATC 5 cut(s) 16, 284, 364, 508, 792
MboII GAAGA 8 cut(s) 16, 34, 304, 413, 488, 545, 640, 1073
MflI RGATCY 1 cut(s) 284
MlsI TGGCCA 1 cut(s) 411
MluCI AATT 6 cut(s) 536, 596, 609, 688, 1004, 1081
MluNI TGGCCA 1 cut(s) 411
MlyI GAGTC 3 cut(s) 104, 318, 880
Mox20I TGGCCA 1 cut(s) 411
MscI TGGCCA 1 cut(s) 411
MseI TTAA 4 cut(s) 104, 138, 570, 782
MslI CAYNNNNRTG 2 cut(s) 639, 1045
Msp20I TGGCCA 1 cut(s) 411
MspI CCGG 4 cut(s) 387, 523, 704, 752
MspR9I CCNGG 1 cut(s) 388
Mva1269I GAATGC 1 cut(s) 846
MwoI GCNNNNNNNGC 5 cut(s) 130, 218, 227, 244, 383
NciI CCSGG 1 cut(s) 388
NcoI CCATGG 1 cut(s) 739
NdeII GATC 5 cut(s) 16, 284, 364, 508, 792
NlaIII CATG 6 cut(s) 234, 247, 377, 503, 743, 772
NlaIV GGNNCC 3 cut(s) 148, 286, 392
NmuCI GTSAC 2 cut(s) 163, 171
NspI RCATGY 1 cut(s) 772
OliI CACNNNNGTG 1 cut(s) 1045
PagI TCATGA 1 cut(s) 499
PciI ACATGT 1 cut(s) 768
PciSI GCTCTTC 1 cut(s) 396
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PctI GAATGC 1 cut(s) 846
PfeI GAWTC 3 cut(s) 201, 666, 850
PinAI ACCGGT 1 cut(s) 522
PkrI GCNGC 2 cut(s) 132, 1092
PleI GAGTC 3 cut(s) 104, 317, 879
PmaCI CACGTG 1 cut(s) 439
PmlI CACGTG 1 cut(s) 439
PpsI GAGTC 3 cut(s) 104, 317, 879
Ppu21I YACGTR 1 cut(s) 439
PpuMI RGGWCCY 1 cut(s) 619
PscI ACATGT 1 cut(s) 768
Psp5II RGGWCCY 1 cut(s) 619
PspCI CACGTG 1 cut(s) 439
PspFI CCCAGC 1 cut(s) 530
PspN4I GGNNCC 3 cut(s) 148, 286, 392
PspPI GGNCC 1 cut(s) 619
PspPPI RGGWCCY 1 cut(s) 619
PstNI CAGNNNCTG 1 cut(s) 356
PsuI RGATCY 1 cut(s) 284
RsaI GTAC 4 cut(s) 392, 567, 815, 1019
RsaNI GTAC 4 cut(s) 391, 566, 814, 1018
RseI CAYNNNNRTG 2 cut(s) 639, 1045
SapI GCTCTTC 1 cut(s) 396
SaqAI TTAA 4 cut(s) 104, 138, 570, 782
SatI GCNGC 2 cut(s) 131, 1091
Sau3AI GATC 5 cut(s) 16, 284, 364, 508, 792
Sau96I GGNCC 1 cut(s) 619
ScaI AGTACT 1 cut(s) 567
SchI GAGTC 3 cut(s) 104, 318, 880
ScrFI CCNGG 1 cut(s) 388
SfaNI GCATC 3 cut(s) 369, 631, 643
SinI GGWCC 1 cut(s) 619
SmiMI CAYNNNNRTG 2 cut(s) 639, 1045
SmlI CTYRAG 1 cut(s) 845
SmoI CTYRAG 1 cut(s) 845
Sse9I AATT 6 cut(s) 536, 596, 609, 688, 1004, 1081
SsiI CCGC 1 cut(s) 1090
SspMI CTAG 2 cut(s) 801, 908
StyD4I CCNGG 1 cut(s) 386
StyI CCWWGG 3 cut(s) 615, 739, 760
TaaI ACNGT 6 cut(s) 146, 163, 169, 352, 466, 859
TaiI ACGT 2 cut(s) 219, 441
TaqI TCGA 2 cut(s) 330, 363
TasI AATT 6 cut(s) 536, 596, 609, 688, 1004, 1081
TatI WGTACW 2 cut(s) 565, 813
TauI GCSGC 1 cut(s) 1093
TfiI GAWTC 3 cut(s) 201, 666, 850
Tru1I TTAA 4 cut(s) 104, 138, 570, 782
Tru9I TTAA 4 cut(s) 104, 138, 570, 782
TscAI CASTG 3 cut(s) 310, 469, 1050
TseFI GTSAC 2 cut(s) 163, 171
TseI GCWGC 1 cut(s) 130
Tsp45I GTSAC 2 cut(s) 163, 171
TspDTI ATGAA 4 cut(s) 17, 488, 609, 690
TspRI CASTG 3 cut(s) 310, 469, 1050
VpaK11BI GGWCC 1 cut(s) 619
XapI RAATTY 4 cut(s) 536, 596, 609, 1004
XceI RCATGY 1 cut(s) 772
XspI CTAG 2 cut(s) 801, 908
ZrmI AGTACT 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.