Rorug06G0398000

leucine-rich repeat extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0012723
Physical Location & Seq
Forward (+)
54356877 .. 54359357
2481 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0398000.1

Sequence Viewer

Length: 1659 bp
ATGTCGACAAGAAAACCTTCATACACAAACCCAAGACCCTGCAAGCACCTTTCAGCTTACAAGCTCAAACATGGCCTGAGTGGGTATAAATCACTCCAAGAGTGCCTCAAGACCACCCCAAATGGAAGGACAAGTGTTGAGAATCCGGGCACAAAGATATCCAGATGCAGTTCCTGTAATGGGTATCAAGGTAGACTCTATTTTTGTCTGATTTGCTCTTCAATCTCATGCTCAAGTCACACCCTTTTGCACACCCAGTCTGAGATTGGCCATGATTTAGCGGTGGACATTGAAAGGGCTGAGCTTTATTGCTGTGCCTGCTCTGATCAGGTCTACGATCCTGATTTTGATAAGACTGTCATGTCTAAACACATGTTGGACTTGAGTTATAGCACAAACAGAAACAGTACAGATAGTATAGGGGAGAGGCTGTGTGAGAGGAAGAGGCTGGGTTCTGGGGTAGAATTGGCAGATTTGAACAAGTCAGGACAGCTGGCTTTGATGAGAGATCATAGGGCTAAATCATGTTATCCATTAGGTTTGAGGGGACTGAATAATTTGGGCAATACTTGTTTCATGAATTCTGTGCTGCAAGCACTGTTGCATGCTCCTCCAATGAGAAATTACTTCTTGAGTGATCGGCATAACCGCAGGACGTGCAGGAAGAGGTCGGCCAACCGGCTCTGTCTCCCTTGTGAGCTTGATGGTATCTTCTCCGCTGTCTACTCGGGTGGTGGTGATCGCACTCCTTATAGCCCGGCTCAATTTCTTTACAGTTGGTGGCAGCATTCAGAAAATCTGGCAAGTTATGAGCAGCAGGATGCTCATGAGTTTTTCATTTCAGTGTTAGATGGGATCCATGAGAGAGAAAGCAAAGCAAGGAACCAAACTAGAGATAGTGGAGATTGCCAGTGTATTACTCATAGGGTCTTCTCTGGACAGTTGAGATCAGATGTCACTTGTATGACTTGTGGATTCACTTCAACAACTTATGACCCTTGTTTGGACATATCGCTCGACTTGGACACAAACCATTGCTCTAATAAGCCTGTCAAACGAAATGACAACAGCAGTACATCTACTCTTCTTGGTTGTTTAGACTTGTTCACCAAACCAGAGAAGTTGGGAACAGACCAGAAGCTGTTTTGTCAAAACTGTCAAGAACCGCGAGACTCATCGAAGCAACTGTCTATTAGAAAGCTCCCTTTGGTGTTGTGTTTGCATATAAAACGATTCGAGCACTCCCTGGTCAGAAAGATGATGAGGAAAATCGACAGCTATATGCATTTTCCTTTCTCCTTAGACATGACTCCATATCTGTCCTCTTCAATTGTCAGAAATAGATTTGGGAATAGAATCTTTGCCTTTGAGGGTGATGATTCCGATAATTCTACAGAATTTGAGATTTTTGCAGTTATTACTCATTCTGGGACGCTAGACTCTGGCCATTATTTAACTTATTTACGCATAAAAGAGCAATGGTATAAGTGTGACAATGCTTGGATTAGTGAGGTTGATGAGAGGGTTGTAAGAGCTTCACAGTGCTATATGATGTTTTATGCGCACAAGACGCTTTACTATAAAGCAAAAGAAGATTTGAGTTGCTTGCCAGTTTCCACACGGAAGGAGGCTTTTCATAGGGTTGCCGGTTGCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005199
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8

Protein Analysis

552

Amino Acids

62.91

Weight (kDa)

8.5

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBP PF02148 56 - 115 5.7e-10 Zn-finger in ubiquitin-hydrolases and other protein
UCH PF00443 182 - 520 2.1e-59 Ubiquitin carboxyl-terminal hydrolase
UCH_1 PF13423 183 - 495 9.7e-20 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012723)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25550
fragaria_vesca FvH4_2g30240
malus_domestica MD08G1135200.v1.1 MD15G1114000.v1.1
prunus_persica Prupe.1G465200_v2.0.a1
pyrus_communis pycom08g11410 pycom15g10230
rosa_chinensis RchiOBHm_Chr6g0312631
rosa_laevigata RLG00000010309
rosa_multiflora Rmu_sc0002859.1_g000007 Rmu_sc0034244.1_g000001 Rmu_ssc0000412.1_g000003
rosa_roxburghii Rroxscaffold_7G00156630
rosa_rugosa Rorug06G0398000
rosa_samantha Rh6AG515300 Rh6BG524800 Rh6CG530200
rosa_wichuraiana Rw6G044750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 361
Acc16I TGCGCA 1 cut(s) 1563
AccI GTMKAC 4 cut(s) 5, 193, 333, 723
AccII CGCG 1 cut(s) 1168
AciI CCGC 4 cut(s) 281, 649, 717, 1166
AclWI GGATC 3 cut(s) 332, 850, 863
AcoI YGGCCR 3 cut(s) 268, 672, 1444
AcsI RAATTY 2 cut(s) 580, 1397
AfaI GTAC 2 cut(s) 409, 1075
AfiI CCNNNNNNNGG 2 cut(s) 180, 1003
AflIII ACRYGT 1 cut(s) 372
AgsI TTSAA 5 cut(s) 222, 293, 478, 984, 1329
AjiI CACGTC 1 cut(s) 657
AjnI CCWGG 1 cut(s) 1245
AluBI AGCT 9 cut(s) 56, 64, 304, 493, 700, 1141, 1201, 1278, 1535
AluI AGCT 9 cut(s) 56, 64, 304, 493, 700, 1141, 1201, 1278, 1535
Alw21I GWGCWC 1 cut(s) 1242
Alw26I GTCTC 2 cut(s) 692, 1164
AlwI GGATC 3 cut(s) 332, 850, 863
AlwNI CAGNNNCTG 2 cut(s) 174, 1141
Ama87I CYCGRG 1 cut(s) 727
AoxI GGCC 4 cut(s) 73, 268, 672, 1444
ApeKI GCWGC 4 cut(s) 589, 784, 814, 1653
ApoI RAATTY 2 cut(s) 580, 1397
ArsI GACNNNNNNTTYG 4 cut(s) 186, 218, 1172, 1204
Asp700I GAANNNNTTC 1 cut(s) 16
AspLEI GCGC 1 cut(s) 1564
AsuC2I CCSGG 2 cut(s) 147, 758
AsuHPI GGTGA 3 cut(s) 749, 1099, 1385
AvaI CYCGRG 1 cut(s) 727
BaeGI GKGCMC 1 cut(s) 152
BalI TGGCCA 2 cut(s) 270, 1446
BamHI GGATCC 1 cut(s) 855
BbsI GAAGAC 1 cut(s) 922
Bbv12I GWGCWC 1 cut(s) 1242
BbvI GCAGC 4 cut(s) 576, 796, 826, 1640
BccI CCATC 2 cut(s) 698, 845
BciT130I CCWGG 1 cut(s) 1247
BclI TGATCA 1 cut(s) 325
BcnI CCSGG 2 cut(s) 147, 758
BcoDI GTCTC 2 cut(s) 692, 1164
BfaI CTAG 2 cut(s) 891, 1436
BfmI CTRYAG 1 cut(s) 1392
BisI GCNGC 4 cut(s) 590, 785, 815, 1654
BlpI GCTNAGC 1 cut(s) 300
BlsI GCNGC 4 cut(s) 591, 786, 816, 1655
Bme1390I CCNGG 3 cut(s) 147, 758, 1247
BmeT110I CYCGRG 1 cut(s) 727
BmgBI CACGTC 1 cut(s) 657
BmiI GGNNCC 2 cut(s) 857, 884
BmrFI CCNGG 3 cut(s) 147, 758, 1247
BmrI ACTGGG 1 cut(s) 250
BmsI GCATC 2 cut(s) 155, 811
BmuI ACTGGG 1 cut(s) 250
BpiI GAAGAC 1 cut(s) 922
Bpu1102I GCTNAGC 1 cut(s) 300
BpuEI CTTGAG 4 cut(s) 92, 217, 403, 652
BpuMI CCSGG 2 cut(s) 147, 758
BsaJI CCNNGG 1 cut(s) 1245
BsaXI ACNNNNNCTCC 2 cut(s) 416, 446
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 1003
Bse118I RCCGGY 2 cut(s) 678, 1646
Bse1I ACTGG 3 cut(s) 256, 910, 1610
Bse3DI GCAATG 2 cut(s) 1033, 1484
BseBI CCWGG 1 cut(s) 1247
BseDI CCNNGG 1 cut(s) 1245
BseGI GGATG 1 cut(s) 826
BseLI CCNNNNNNNGG 2 cut(s) 180, 1003
BseMI GCAATG 2 cut(s) 1033, 1484
BseMII CTCAG 3 cut(s) 68, 252, 291
BseNI ACTGG 3 cut(s) 256, 910, 1610
BseRI GAGGAG 1 cut(s) 600
BseSI GKGCMC 1 cut(s) 152
BseXI GCAGC 4 cut(s) 576, 796, 826, 1640
BseYI CCCAGC 1 cut(s) 448
BsgI GTGCAG 1 cut(s) 679
Bsh1236I CGCG 1 cut(s) 1168
BshFI GGCC 4 cut(s) 75, 270, 674, 1446
BsiHKAI GWGCWC 1 cut(s) 1242
BsiHKCI CYCGRG 1 cut(s) 727
BsiSI CCGG 4 cut(s) 146, 679, 758, 1647
BslFI GGGAC 2 cut(s) 561, 1444
BslI CCNNNNNNNGG 2 cut(s) 180, 1003
BsmAI GTCTC 2 cut(s) 692, 1164
BsmFI GGGAC 2 cut(s) 561, 1444
BsmI GAATGC 1 cut(s) 787
BsnI GGCC 4 cut(s) 75, 270, 674, 1446
BsoBI CYCGRG 1 cut(s) 727
Bsp1286I GDGCHC 2 cut(s) 152, 1242
Bsp143I GATC 7 cut(s) 325, 337, 508, 637, 739, 855, 947
Bsp1720I GCTNAGC 1 cut(s) 300
BspACI CCGC 4 cut(s) 281, 649, 717, 1166
BspANI GGCC 4 cut(s) 75, 270, 674, 1446
BspCNI CTCAG 3 cut(s) 69, 253, 292
BspFNI CGCG 1 cut(s) 1168
BspHI TCATGA 2 cut(s) 576, 826
BspLI GGNNCC 2 cut(s) 857, 884
BspPI GGATC 3 cut(s) 332, 850, 863
BspQI GCTCTTC 1 cut(s) 223
BsrDI GCAATG 2 cut(s) 1033, 1484
BsrFI RCCGGY 2 cut(s) 678, 1646
BsrI ACTGG 3 cut(s) 256, 910, 1610
BssAI RCCGGY 2 cut(s) 678, 1646
BssECI CCNNGG 1 cut(s) 1245
BssMI GATC 7 cut(s) 325, 337, 508, 637, 739, 855, 947
Bst2UI CCWGG 1 cut(s) 1247
Bst4CI ACNGT 8 cut(s) 358, 407, 600, 776, 942, 1157, 1188, 1542
Bst6I CTCTTC 5 cut(s) 223, 437, 659, 1089, 1330
BstAPI GCANNNNNTGC 1 cut(s) 657
BstC8I GCNNGC 6 cut(s) 44, 319, 495, 594, 606, 1607
BstDEI CTNAG 4 cut(s) 77, 261, 300, 1300
BstF5I GGATG 1 cut(s) 826
BstFNI CGCG 1 cut(s) 1168
BstHHI GCGC 1 cut(s) 1564
BstKTI GATC 7 cut(s) 328, 340, 511, 640, 742, 858, 950
BstMAI GTCTC 2 cut(s) 692, 1164
BstMBI GATC 7 cut(s) 325, 337, 508, 637, 739, 855, 947
BstMWI GCNNNNNNNGC 3 cut(s) 318, 657, 1570
BstNI CCWGG 1 cut(s) 1247
BstNSI RCATGY 2 cut(s) 376, 608
BstSCI CCNGG 3 cut(s) 145, 756, 1245
BstSFI CTRYAG 1 cut(s) 1392
BstSLI GKGCMC 1 cut(s) 152
BstUI CGCG 1 cut(s) 1168
BstV1I GCAGC 4 cut(s) 576, 796, 826, 1640
BstV2I GAAGAC 1 cut(s) 922
BstX2I RGATCY 1 cut(s) 855
BstYI RGATCY 1 cut(s) 855
BsuRI GGCC 4 cut(s) 75, 270, 674, 1446
BtrI CACGTC 1 cut(s) 657
BtsCI GGATG 1 cut(s) 826
BtsIMutI CAGTG 4 cut(s) 596, 849, 917, 1547
Cac8I GCNNGC 6 cut(s) 44, 319, 495, 594, 606, 1607
CaiI CAGNNNCTG 2 cut(s) 174, 1141
CciI TCATGA 2 cut(s) 576, 826
CfoI GCGC 1 cut(s) 1564
Cfr10I RCCGGY 2 cut(s) 678, 1646
CseI GACGC 2 cut(s) 1441, 1579
Csp6I GTAC 2 cut(s) 408, 1074
CviQI GTAC 2 cut(s) 408, 1074
DdeI CTNAG 4 cut(s) 77, 261, 300, 1300
DpnI GATC 7 cut(s) 327, 339, 510, 639, 741, 857, 949
DpnII GATC 7 cut(s) 325, 337, 508, 637, 739, 855, 947
DrdI GACNNNNNNGTC 1 cut(s) 361
DseDI GACNNNNNNGTC 1 cut(s) 361
EaeI YGGCCR 3 cut(s) 268, 672, 1444
Eam1104I CTCTTC 5 cut(s) 223, 437, 659, 1089, 1330
EarI CTCTTC 5 cut(s) 223, 437, 659, 1089, 1330
Eco32I GATATC 1 cut(s) 159
Eco88I CYCGRG 1 cut(s) 727
EcoRI GAATTC 1 cut(s) 580
EcoRII CCWGG 1 cut(s) 1245
EcoRV GATATC 1 cut(s) 159
EcoT22I ATGCAT 1 cut(s) 1287
FalI AAGNNNNNCTT 1 cut(s) 33
FaqI GGGAC 2 cut(s) 561, 1444
FbaI TGATCA 1 cut(s) 325
FblI GTMKAC 4 cut(s) 5, 193, 333, 723
Fnu4HI GCNGC 4 cut(s) 590, 785, 815, 1654
FokI GGATG 1 cut(s) 833
Fsp4HI GCNGC 4 cut(s) 590, 785, 815, 1654
FspAI RTGCGCAY 1 cut(s) 1563
FspBI CTAG 2 cut(s) 891, 1436
FspI TGCGCA 1 cut(s) 1563
GlaI GCGC 1 cut(s) 1563
GluI GCNGC 4 cut(s) 590, 785, 815, 1654
GsaI CCCAGC 1 cut(s) 452
HaeIII GGCC 4 cut(s) 75, 270, 674, 1446
HapII CCGG 4 cut(s) 146, 679, 758, 1647
HgaI GACGC 2 cut(s) 1441, 1579
HhaI GCGC 1 cut(s) 1564
Hin6I GCGC 1 cut(s) 1562
HinP1I GCGC 1 cut(s) 1562
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 9 cut(s) 142, 195, 975, 1172, 1233, 1309, 1356, 1379, 1439
HpaII CCGG 4 cut(s) 146, 679, 758, 1647
HphI GGTGA 3 cut(s) 749, 1099, 1385
Hpy166II GTNNAC 6 cut(s) 6, 194, 286, 334, 724, 1107
Hpy188I TCNGA 8 cut(s) 210, 262, 325, 793, 952, 1253, 1337, 1384
Hpy188III TCNNGA 9 cut(s) 109, 162, 341, 486, 577, 631, 827, 936, 1160
Hpy8I GTNNAC 6 cut(s) 6, 194, 286, 334, 724, 1107
HpyAV CCTTC 3 cut(s) 27, 120, 1618
HpyCH4III ACNGT 8 cut(s) 358, 407, 600, 776, 942, 1157, 1188, 1542
HpyCH4IV ACGT 1 cut(s) 656
HpyCH4V TGCA 9 cut(s) 42, 168, 250, 592, 604, 660, 1222, 1285, 1412
HpyF10VI GCNNNNNNNGC 3 cut(s) 318, 657, 1570
HpyF3I CTNAG 4 cut(s) 77, 261, 300, 1300
HpySE526I ACGT 1 cut(s) 656
HspAI GCGC 1 cut(s) 1562
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 7 cut(s) 325, 337, 508, 637, 739, 855, 947
LguI GCTCTTC 1 cut(s) 223
LmnI GCTCC 2 cut(s) 613, 1206
Lsp1109I GCAGC 4 cut(s) 576, 796, 826, 1640
LweI GCATC 2 cut(s) 155, 811
MaeI CTAG 2 cut(s) 891, 1436
MaeII ACGT 1 cut(s) 656
MaeIII GTNAC 3 cut(s) 236, 955, 1490
MalI GATC 7 cut(s) 327, 339, 510, 639, 741, 857, 949
MboI GATC 7 cut(s) 325, 337, 508, 637, 739, 855, 947
MboII GAAGA 8 cut(s) 210, 454, 676, 703, 922, 1076, 1317, 1604
MfeI CAATTG 1 cut(s) 1329
MflI RGATCY 1 cut(s) 855
MhlI GDGCHC 2 cut(s) 152, 1242
MlsI TGGCCA 2 cut(s) 270, 1446
MluCI AATT 8 cut(s) 464, 556, 580, 622, 764, 1329, 1387, 1397
MluNI TGGCCA 2 cut(s) 270, 1446
MlyI GAGTC 4 cut(s) 189, 1166, 1303, 1433
MmeI TCCRAC 1 cut(s) 357
Mox20I TGGCCA 2 cut(s) 270, 1446
Mph1103I ATGCAT 1 cut(s) 1287
MroXI GAANNNNTTC 1 cut(s) 16
MscI TGGCCA 2 cut(s) 270, 1446
MseI TTAA 1 cut(s) 1454
MslI CAYNNNNRTG 2 cut(s) 842, 962
Msp20I TGGCCA 2 cut(s) 270, 1446
MspA1I CMGCKG 2 cut(s) 493, 719
MspI CCGG 4 cut(s) 146, 679, 758, 1647
MspR9I CCNGG 3 cut(s) 147, 758, 1247
MunI CAATTG 1 cut(s) 1329
Mva1269I GAATGC 1 cut(s) 787
MvaI CCWGG 1 cut(s) 1247
MvnI CGCG 1 cut(s) 1168
MwoI GCNNNNNNNGC 3 cut(s) 318, 657, 1570
NciI CCSGG 2 cut(s) 147, 758
NdeII GATC 7 cut(s) 325, 337, 508, 637, 739, 855, 947
NlaIV GGNNCC 2 cut(s) 857, 884
NmuCI GTSAC 3 cut(s) 236, 955, 1490
NsbI TGCGCA 1 cut(s) 1563
NsiI ATGCAT 1 cut(s) 1287
NspI RCATGY 2 cut(s) 376, 608
PaeI GCATGC 1 cut(s) 608
PagI TCATGA 2 cut(s) 576, 826
PciI ACATGT 1 cut(s) 372
PciSI GCTCTTC 1 cut(s) 223
PctI GAATGC 1 cut(s) 787
PdmI GAANNNNTTC 1 cut(s) 16
PfeI GAWTC 5 cut(s) 142, 975, 1233, 1356, 1379
PkrI GCNGC 4 cut(s) 591, 786, 816, 1655
PleI GAGTC 4 cut(s) 189, 1166, 1303, 1433
PpsI GAGTC 4 cut(s) 189, 1166, 1303, 1433
PscI ACATGT 1 cut(s) 372
Psp6I CCWGG 1 cut(s) 1245
PspFI CCCAGC 1 cut(s) 448
PspGI CCWGG 1 cut(s) 1245
PspN4I GGNNCC 2 cut(s) 857, 884
PstNI CAGNNNCTG 2 cut(s) 174, 1141
PsuI RGATCY 1 cut(s) 855
PvuII CAGCTG 1 cut(s) 493
RsaI GTAC 2 cut(s) 409, 1075
RsaNI GTAC 2 cut(s) 408, 1074
RseI CAYNNNNRTG 2 cut(s) 842, 962
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 223
SaqAI TTAA 1 cut(s) 1454
SatI GCNGC 4 cut(s) 590, 785, 815, 1654
Sau3AI GATC 7 cut(s) 325, 337, 508, 637, 739, 855, 947
SchI GAGTC 4 cut(s) 189, 1166, 1303, 1433
ScrFI CCNGG 3 cut(s) 147, 758, 1247
SduI GDGCHC 2 cut(s) 152, 1242
SfaNI GCATC 2 cut(s) 155, 811
SfcI CTRYAG 1 cut(s) 1392
SmiMI CAYNNNNRTG 2 cut(s) 842, 962
SmlI CTYRAG 4 cut(s) 107, 232, 382, 631
SmoI CTYRAG 4 cut(s) 107, 232, 382, 631
SphI GCATGC 1 cut(s) 608
Sse9I AATT 8 cut(s) 464, 556, 580, 622, 764, 1329, 1387, 1397
SsiI CCGC 4 cut(s) 281, 649, 717, 1166
SspMI CTAG 2 cut(s) 891, 1436
StyD4I CCNGG 3 cut(s) 145, 756, 1245
TaaI ACNGT 8 cut(s) 358, 407, 600, 776, 942, 1157, 1188, 1542
TaiI ACGT 1 cut(s) 659
TaqI TCGA 5 cut(s) 5, 1017, 1178, 1236, 1272
TasI AATT 8 cut(s) 464, 556, 580, 622, 764, 1329, 1387, 1397
TatI WGTACW 2 cut(s) 407, 1073
TfiI GAWTC 5 cut(s) 142, 975, 1233, 1356, 1379
Tru1I TTAA 1 cut(s) 1454
Tru9I TTAA 1 cut(s) 1454
TscAI CASTG 4 cut(s) 603, 849, 917, 1547
TseFI GTSAC 3 cut(s) 236, 955, 1490
TseI GCWGC 4 cut(s) 589, 784, 814, 1653
Tsp45I GTSAC 3 cut(s) 236, 955, 1490
TspDTI ATGAA 5 cut(s) 9, 565, 593, 826, 1625
TspGWI ACGGA 1 cut(s) 1636
TspRI CASTG 4 cut(s) 603, 849, 917, 1547
XapI RAATTY 2 cut(s) 580, 1397
XceI RCATGY 2 cut(s) 376, 608
XcmI CCANNNNNNNNNTGG 1 cut(s) 263
XmiI GTMKAC 4 cut(s) 5, 193, 333, 723
XmnI GAANNNNTTC 1 cut(s) 16
XspI CTAG 2 cut(s) 891, 1436
Zsp2I ATGCAT 1 cut(s) 1287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.