AT5G40950

ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009984
Physical Location & Seq
Forward (+)
16410782 .. 16412114
1333 bp
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UTR
Exon/CDS
Intron
AT5G40950.1

Sequence Viewer

Length: 597 bp
ATGGCGATGGCAACATCGATGAGTTTGAATCTAATTGGGGCATTCAAAGGCTTGTCTCTTTCTTCGACTTCGTCTTTTCTTAGAGGCGATTTGAGTTTTTCCCCCAAAACCTCTTTCACGGTGACTCTTCCTCTGGAAAATCTTCAAGCTCCGATTCCGTTGACAATTGAATCGGCCCATAAGAAAGGAGCTGGTAGCACCAAGAACGGTCGTGATTCTCCTGGGCAACGACTCGGCGTCAAGATCTACGGTGACCAAGTTGCTAAACCTGGTGCTATCATTGTTCGTCAACGTGGCACTAAGTTCCATGCTGGGAAAAATGTTGGGATTGGTAAAGATCATACCATCTTCTCTTTAATCGATGGATTAGTCAAGTTCGAGAAGTTTGGTCCTGACAGGAAGAAGATAAGTGTGTATCCAAGAGAAATTGTACCAGAGAATCCCAATAGCTACAGAGCAAGAAAGAGAGAAAACTTCAGATTGCAAAGGGAGAAGAAGAAGGCGAGACGCGAGAATTACTCGTACACACTTCCTACACCAGAACTTGTTCTTGCATCTGCCTCAGTCGATGATGCTGAAGCCAATCCGGAGTGCTAG

Protein Analysis

198

Amino Acids

21.74

Weight (kDa)

9.96

Isoelectric Point (pI)

29.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27 PF01016 59 - 138 1.5e-35 Ribosomal L27 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 510
AccIII TCCGGA 1 cut(s) 586
AcuI CTGAAG 2 cut(s) 460, 597
AcyI GRCGYC 1 cut(s) 237
AfaI GTAC 2 cut(s) 432, 524
AgsI TTSAA 4 cut(s) 28, 46, 146, 170
AhdI GACNNNNNGTC 1 cut(s) 236
AjnI CCWGG 2 cut(s) 220, 268
AjuI GAANNNNNNNTTGG 2 cut(s) 98, 130
AluBI AGCT 3 cut(s) 149, 191, 450
AluI AGCT 3 cut(s) 149, 191, 450
Alw26I GTCTC 2 cut(s) 60, 499
Aor13HI TCCGGA 1 cut(s) 586
AoxI GGCC 1 cut(s) 174
Asp700I GAANNNNTTC 2 cut(s) 141, 546
AspS9I GGNCC 2 cut(s) 175, 389
AsuHPI GGTGA 2 cut(s) 133, 263
AvaII GGWCC 1 cut(s) 389
BccI CCATC 2 cut(s) 353, 356
BciT130I CCWGG 2 cut(s) 222, 270
BciVI GTATCC 1 cut(s) 426
BcoDI GTCTC 2 cut(s) 60, 499
BfaI CTAG 1 cut(s) 595
BfmI CTRYAG 1 cut(s) 451
BfuI GTATCC 1 cut(s) 426
BglII AGATCT 1 cut(s) 243
Bme1390I CCNGG 2 cut(s) 222, 270
Bme18I GGWCC 1 cut(s) 389
BmeRI GACNNNNNGTC 1 cut(s) 236
BmgT120I GGNCC 2 cut(s) 175, 389
BmrFI CCNGG 2 cut(s) 222, 270
BmsI GCATC 2 cut(s) 562, 563
BplI GAGNNNNNCTC 2 cut(s) 503, 535
Bsa29I ATCGAT 2 cut(s) 17, 360
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 221
BsaWI WCCGGW 1 cut(s) 586
BseAI TCCGGA 1 cut(s) 586
BseBI CCWGG 2 cut(s) 222, 270
BseCI ATCGAT 2 cut(s) 17, 360
BseDI CCNNGG 1 cut(s) 221
BseMII CTCAG 1 cut(s) 576
BseYI CCCAGC 1 cut(s) 311
Bsh1236I CGCG 1 cut(s) 510
Bsh1285I CGRYCG 1 cut(s) 211
BshFI GGCC 1 cut(s) 176
BshVI ATCGAT 2 cut(s) 17, 360
BsiEI CGRYCG 1 cut(s) 211
BsiSI CCGG 1 cut(s) 587
BsmAI GTCTC 2 cut(s) 60, 499
BsmBI CGTCTC 1 cut(s) 499
BsmI GAATGC 1 cut(s) 41
BsnI GGCC 1 cut(s) 176
Bsp13I TCCGGA 1 cut(s) 586
Bsp143I GATC 2 cut(s) 243, 337
BspANI GGCC 1 cut(s) 176
BspCNI CTCAG 1 cut(s) 575
BspDI ATCGAT 2 cut(s) 17, 360
BspEI TCCGGA 1 cut(s) 586
BspFNI CGCG 1 cut(s) 510
BssECI CCNNGG 1 cut(s) 221
BssMI GATC 2 cut(s) 243, 337
BssNI GRCGYC 1 cut(s) 237
Bst2UI CCWGG 2 cut(s) 222, 270
Bst4CI ACNGT 3 cut(s) 121, 209, 251
Bst6I CTCTTC 1 cut(s) 132
BstACI GRCGYC 1 cut(s) 237
BstDEI CTNAG 3 cut(s) 80, 300, 562
BstEII GGTNACC 1 cut(s) 251
BstFNI CGCG 1 cut(s) 510
BstKTI GATC 2 cut(s) 246, 340
BstMAI GTCTC 2 cut(s) 60, 499
BstMBI GATC 2 cut(s) 243, 337
BstMCI CGRYCG 1 cut(s) 211
BstNI CCWGG 2 cut(s) 222, 270
BstPI GGTNACC 1 cut(s) 251
BstSCI CCNGG 2 cut(s) 220, 268
BstSFI CTRYAG 1 cut(s) 451
BstUI CGCG 1 cut(s) 510
BstX2I RGATCY 1 cut(s) 243
BstYI RGATCY 1 cut(s) 243
Bsu15I ATCGAT 2 cut(s) 17, 360
BsuI GTATCC 1 cut(s) 426
BsuRI GGCC 1 cut(s) 176
BsuTUI ATCGAT 2 cut(s) 17, 360
BtgZI GCGATG 1 cut(s) 20
Cfr13I GGNCC 2 cut(s) 175, 389
ClaI ATCGAT 2 cut(s) 17, 360
CseI GACGC 2 cut(s) 226, 516
CsiI ACCWGGT 1 cut(s) 268
Csp6I GTAC 2 cut(s) 431, 523
CviAII CATG 1 cut(s) 308
CviJI RGCY 6 cut(s) 51, 149, 176, 191, 450, 581
CviKI_1 RGCY 6 cut(s) 51, 149, 176, 191, 450, 581
CviQI GTAC 2 cut(s) 431, 523
DdeI CTNAG 3 cut(s) 80, 300, 562
DpnI GATC 2 cut(s) 245, 339
DpnII GATC 2 cut(s) 243, 337
DriI GACNNNNNGTC 1 cut(s) 236
Eam1104I CTCTTC 1 cut(s) 132
Eam1105I GACNNNNNGTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 389
Eco57I CTGAAG 2 cut(s) 460, 597
Eco91I GGTNACC 1 cut(s) 251
EcoO65I GGTNACC 1 cut(s) 251
EcoRII CCWGG 2 cut(s) 220, 268
Esp3I CGTCTC 1 cut(s) 499
FaeI CATG 1 cut(s) 311
FaiI YATR 3 cut(s) 180, 309, 342
FatI CATG 1 cut(s) 307
FspBI CTAG 1 cut(s) 595
GsaI CCCAGC 1 cut(s) 315
HaeIII GGCC 1 cut(s) 176
HapII CCGG 1 cut(s) 587
HgaI GACGC 2 cut(s) 226, 516
Hin1I GRCGYC 1 cut(s) 237
Hin1II CATG 1 cut(s) 311
HincII GTYRAC 2 cut(s) 162, 290
HindII GTYRAC 2 cut(s) 162, 290
HinfI GANTC 7 cut(s) 28, 124, 154, 170, 215, 231, 439
HpaII CCGG 1 cut(s) 587
HphI GGTGA 2 cut(s) 133, 263
Hpy166II GTNNAC 3 cut(s) 162, 290, 525
Hpy188I TCNGA 2 cut(s) 153, 479
Hpy188III TCNNGA 6 cut(s) 134, 212, 241, 379, 392, 587
Hpy8I GTNNAC 3 cut(s) 162, 290, 525
HpyAV CCTTC 1 cut(s) 493
HpyCH4III ACNGT 3 cut(s) 121, 209, 251
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 2 cut(s) 484, 554
HpyF3I CTNAG 3 cut(s) 80, 300, 562
HpySE526I ACGT 1 cut(s) 292
Hsp92I GRCGYC 1 cut(s) 237
Hsp92II CATG 1 cut(s) 311
Kpn2I TCCGGA 1 cut(s) 586
Kzo9I GATC 2 cut(s) 243, 337
LmnI GCTCC 2 cut(s) 154, 188
LweI GCATC 2 cut(s) 562, 563
MabI ACCWGGT 1 cut(s) 268
MaeI CTAG 1 cut(s) 595
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 2 cut(s) 121, 251
MalI GATC 2 cut(s) 245, 339
MboI GATC 2 cut(s) 243, 337
MboII GAAGA 8 cut(s) 54, 119, 134, 340, 412, 415, 505, 508
MfeI CAATTG 1 cut(s) 165
MflI RGATCY 1 cut(s) 243
MluCI AATT 4 cut(s) 33, 165, 426, 514
MlyI GAGTC 2 cut(s) 118, 225
MnlI CCTC 4 cut(s) 77, 121, 141, 571
MroI TCCGGA 1 cut(s) 586
MroXI GAANNNNTTC 2 cut(s) 141, 546
MseI TTAA 1 cut(s) 356
MspI CCGG 1 cut(s) 587
MspR9I CCNGG 2 cut(s) 222, 270
MunI CAATTG 1 cut(s) 165
Mva1269I GAATGC 1 cut(s) 41
MvaI CCWGG 2 cut(s) 222, 270
MvnI CGCG 1 cut(s) 510
NdeII GATC 2 cut(s) 243, 337
NlaIII CATG 1 cut(s) 311
NmeAIII GCCGAG 1 cut(s) 213
NmuCI GTSAC 2 cut(s) 121, 251
PctI GAATGC 1 cut(s) 41
PdmI GAANNNNTTC 2 cut(s) 141, 546
PfeI GAWTC 5 cut(s) 28, 154, 170, 215, 439
PflFI GACNNNGTC 1 cut(s) 70
PleI GAGTC 2 cut(s) 118, 225
PpsI GAGTC 2 cut(s) 118, 225
Psp6I CCWGG 2 cut(s) 220, 268
PspEI GGTNACC 1 cut(s) 251
PspFI CCCAGC 1 cut(s) 311
PspGI CCWGG 2 cut(s) 220, 268
PspPI GGNCC 2 cut(s) 175, 389
PsuI RGATCY 1 cut(s) 243
PsyI GACNNNGTC 1 cut(s) 70
RsaI GTAC 2 cut(s) 432, 524
RsaNI GTAC 2 cut(s) 431, 523
SaqAI TTAA 1 cut(s) 356
Sau3AI GATC 2 cut(s) 243, 337
Sau96I GGNCC 2 cut(s) 175, 389
SchI GAGTC 2 cut(s) 118, 225
ScrFI CCNGG 2 cut(s) 222, 270
SetI ASST 6 cut(s) 113, 151, 193, 271, 295, 452
SexAI ACCWGGT 1 cut(s) 268
SfaNI GCATC 2 cut(s) 562, 563
SfcI CTRYAG 1 cut(s) 451
SinI GGWCC 1 cut(s) 389
Sse9I AATT 4 cut(s) 33, 165, 426, 514
SspMI CTAG 1 cut(s) 595
StyD4I CCNGG 2 cut(s) 220, 268
TaaI ACNGT 3 cut(s) 121, 209, 251
TaiI ACGT 1 cut(s) 295
TaqI TCGA 5 cut(s) 17, 65, 360, 378, 567
TasI AATT 4 cut(s) 33, 165, 426, 514
TfiI GAWTC 5 cut(s) 28, 154, 170, 215, 439
Tru1I TTAA 1 cut(s) 356
Tru9I TTAA 1 cut(s) 356
TseFI GTSAC 2 cut(s) 121, 251
Tsp45I GTSAC 2 cut(s) 121, 251
TspGWI ACGGA 1 cut(s) 147
Tth111I GACNNNGTC 1 cut(s) 70
VpaK11BI GGWCC 1 cut(s) 389
XmnI GAANNNNTTC 2 cut(s) 141, 546
XspI CTAG 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.