MD00G1153700.v1.1

50S ribosomal protein L27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0009984
Physical Location & Seq
Reverse (-)
33645495 .. 33646706
1212 bp
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UTR
Exon/CDS
Intron
MD00G1153700.v1.1.491

Sequence Viewer

Length: 528 bp
ATGGCGGTTGCGTCCATGAGTTTCAACGTAGTCGGAGCTTTCAGGGGTCTGTCTCTTCGCCCAACCTCCACCTCCAGCTCGTCGTCCTCCTCCTTCTTCAGAGGTGATTTGGGTTCTCTGCAACTGGGTCCCAAGCTATCGTTAGTGTCGTTGGGCCCGCAGAGACTTCCATTGACGATTCAAAACGCGCACAAGAAAGGAGCTGGGAGTACCAAGAACGGACGTGATTCCAAAGGCCAGAGGCTCTGCGTCAAGATTTACGACGACCAGGTCGCCAAGGCTGGCTCCATCATTGTCCGCCAGCGTGGCACCAAGATTAGTGTTTACCCTCGAGAAGTCCAGCCGGAGAACCCCAACAGCTACCGGGTTAAAAAGAGGGAGAACTTCAGGCTACATCGTGAACGTAAGAAAGCGAGAAAGGAGGGCTACATCATTCAAAACAAACCCGAGCTACTACTTGCTTCTGCTAATGCTGCAGATAGGGCTGGGCACGGGCCGGGGCGGGCCGGGCCGAACCTAAGTTTGTAG

Protein Analysis

176

Amino Acids

19.01

Weight (kDa)

11.02

Isoelectric Point (pI)

23.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27 PF01016 63 - 106 3.5e-17 Ribosomal L27 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 269
AccB1I GGYRCC 1 cut(s) 308
AccII CGCG 1 cut(s) 188
AciI CCGC 4 cut(s) 5, 158, 298, 502
AcuI CTGAAG 2 cut(s) 82, 370
AfaI GTAC 1 cut(s) 211
AgsI TTSAA 3 cut(s) 25, 182, 437
AjiI CACGTC 1 cut(s) 224
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 6 cut(s) 38, 78, 136, 203, 360, 451
AluI AGCT 6 cut(s) 38, 78, 136, 203, 360, 451
Alw26I GTCTC 2 cut(s) 57, 157
Ama87I CYCGRG 2 cut(s) 330, 446
AoxI GGCC 5 cut(s) 154, 235, 494, 504, 509
ApaI GGGCCC 1 cut(s) 158
ApeKI GCWGC 1 cut(s) 473
AspLEI GCGC 1 cut(s) 190
AspS9I GGNCC 6 cut(s) 128, 154, 155, 494, 504, 509
AsuC2I CCSGG 3 cut(s) 365, 498, 508
AsuHPI GGTGA 1 cut(s) 116
AvaI CYCGRG 2 cut(s) 330, 446
AvaII GGWCC 1 cut(s) 128
BaeGI GKGCMC 2 cut(s) 158, 492
BanI GGYRCC 1 cut(s) 308
BanII GRGCYC 1 cut(s) 158
BbvI GCAGC 1 cut(s) 460
BccI CCATC 1 cut(s) 296
BciT130I CCWGG 1 cut(s) 269
BcnI CCSGG 3 cut(s) 365, 498, 508
BcoDI GTCTC 2 cut(s) 57, 157
BfmI CTRYAG 1 cut(s) 474
BglI GCCNNNNNGGC 1 cut(s) 306
BisI GCNGC 1 cut(s) 474
BlsI GCNGC 1 cut(s) 475
Bme1390I CCNGG 4 cut(s) 269, 365, 498, 508
Bme18I GGWCC 1 cut(s) 128
BmeT110I CYCGRG 2 cut(s) 330, 446
BmgBI CACGTC 1 cut(s) 224
BmgT120I GGNCC 6 cut(s) 128, 154, 155, 494, 504, 509
BmiI GGNNCC 5 cut(s) 129, 130, 156, 286, 310
BmrFI CCNGG 4 cut(s) 269, 365, 498, 508
BmrI ACTGGG 1 cut(s) 134
BmuI ACTGGG 1 cut(s) 134
BpmI CTGGAG 1 cut(s) 58
BpuMI CCSGG 3 cut(s) 365, 498, 508
BsaJI CCNNGG 2 cut(s) 276, 497
Bse1I ACTGG 1 cut(s) 129
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 2 cut(s) 276, 497
BseNI ACTGG 1 cut(s) 129
BseRI GAGGAG 1 cut(s) 79
BseSI GKGCMC 2 cut(s) 158, 492
BseXI GCAGC 1 cut(s) 460
BseYI CCCAGC 2 cut(s) 203, 485
Bsh1236I CGCG 1 cut(s) 188
BshFI GGCC 5 cut(s) 156, 237, 496, 506, 511
BshNI GGYRCC 1 cut(s) 308
BsiHKCI CYCGRG 2 cut(s) 330, 446
BsiSI CCGG 4 cut(s) 344, 364, 497, 507
BslFI GGGAC 1 cut(s) 114
BsmAI GTCTC 2 cut(s) 57, 157
BsmFI GGGAC 1 cut(s) 114
BsnI GGCC 5 cut(s) 156, 237, 496, 506, 511
BsoBI CYCGRG 2 cut(s) 330, 446
Bsp120I GGGCCC 1 cut(s) 154
Bsp1286I GDGCHC 2 cut(s) 158, 492
BspACI CCGC 4 cut(s) 5, 158, 298, 502
BspANI GGCC 5 cut(s) 156, 237, 496, 506, 511
BspFNI CGCG 1 cut(s) 188
BspLI GGNNCC 5 cut(s) 129, 130, 156, 286, 310
BspMAI CTGCAG 1 cut(s) 478
BspT107I GGYRCC 1 cut(s) 308
BsrI ACTGG 1 cut(s) 129
BssECI CCNNGG 2 cut(s) 276, 497
BssT1I CCWWGG 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 269
Bst6I CTCTTC 1 cut(s) 60
BstC8I GCNNGC 4 cut(s) 158, 283, 302, 504
BstDEI CTNAG 1 cut(s) 518
BstFNI CGCG 1 cut(s) 188
BstHHI GCGC 1 cut(s) 190
BstMAI GTCTC 2 cut(s) 57, 157
BstMWI GCNNNNNNNGC 4 cut(s) 306, 473, 482, 508
BstNI CCWGG 1 cut(s) 269
BstSCI CCNGG 4 cut(s) 267, 363, 496, 506
BstSFI CTRYAG 1 cut(s) 474
BstSLI GKGCMC 2 cut(s) 158, 492
BstUI CGCG 1 cut(s) 188
BstV1I GCAGC 1 cut(s) 460
BsuRI GGCC 5 cut(s) 156, 237, 496, 506, 511
BtrI CACGTC 1 cut(s) 224
Cac8I GCNNGC 4 cut(s) 158, 283, 302, 504
CfoI GCGC 1 cut(s) 190
Cfr13I GGNCC 6 cut(s) 128, 154, 155, 494, 504, 509
CseI GACGC 1 cut(s) 238
CsiI ACCWGGT 1 cut(s) 267
Csp6I GTAC 1 cut(s) 210
CviAII CATG 1 cut(s) 16
CviQI GTAC 1 cut(s) 210
DdeI CTNAG 1 cut(s) 518
DrdI GACNNNNNNGTC 1 cut(s) 269
DseDI GACNNNNNNGTC 1 cut(s) 269
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
EciI GGCGGA 1 cut(s) 287
Eco130I CCWWGG 1 cut(s) 276
Eco24I GRGCYC 1 cut(s) 158
Eco47I GGWCC 1 cut(s) 128
Eco57I CTGAAG 2 cut(s) 82, 370
Eco88I CYCGRG 2 cut(s) 330, 446
EcoO109I RGGNCCY 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 267
EcoT14I CCWWGG 1 cut(s) 276
EcoT38I GRGCYC 1 cut(s) 158
ErhI CCWWGG 1 cut(s) 276
FaeI CATG 1 cut(s) 19
FaiI YATR 1 cut(s) 17
FaqI GGGAC 1 cut(s) 114
FatI CATG 1 cut(s) 15
FauI CCCGC 2 cut(s) 165, 495
Fnu4HI GCNGC 1 cut(s) 474
FriOI GRGCYC 1 cut(s) 158
Fsp4HI GCNGC 1 cut(s) 474
GlaI GCGC 1 cut(s) 189
GluI GCNGC 1 cut(s) 474
GsaI CCCAGC 2 cut(s) 207, 489
GsuI CTGGAG 1 cut(s) 58
HaeIII GGCC 5 cut(s) 156, 237, 496, 506, 511
HapII CCGG 4 cut(s) 344, 364, 497, 507
HgaI GACGC 1 cut(s) 238
HhaI GCGC 1 cut(s) 190
Hin1II CATG 1 cut(s) 19
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HinfI GANTC 2 cut(s) 178, 227
HpaII CCGG 4 cut(s) 344, 364, 497, 507
HphI GGTGA 1 cut(s) 116
Hpy166II GTNNAC 2 cut(s) 325, 401
Hpy188I TCNGA 2 cut(s) 35, 101
Hpy188III TCNNGA 3 cut(s) 253, 332, 398
Hpy8I GTNNAC 2 cut(s) 325, 401
Hpy99I CGWCG 2 cut(s) 85, 266
HpyAV CCTTC 1 cut(s) 103
HpyCH4IV ACGT 3 cut(s) 27, 223, 403
HpyCH4V TGCA 2 cut(s) 121, 476
HpyF10VI GCNNNNNNNGC 4 cut(s) 306, 473, 482, 508
HpyF3I CTNAG 1 cut(s) 518
HpySE526I ACGT 3 cut(s) 27, 223, 403
Hsp92II CATG 1 cut(s) 19
HspAI GCGC 1 cut(s) 188
KflI GGGWCCC 1 cut(s) 128
LmnI GCTCC 3 cut(s) 35, 200, 290
Lsp1109I GCAGC 1 cut(s) 460
MabI ACCWGGT 1 cut(s) 267
MaeII ACGT 3 cut(s) 27, 223, 403
MboII GAAGA 2 cut(s) 47, 88
MhlI GDGCHC 2 cut(s) 158, 492
MmeI TCCRAC 1 cut(s) 13
MnlI CCTC 9 cut(s) 76, 82, 95, 97, 100, 234, 339, 369, 415
MseI TTAA 1 cut(s) 369
MspI CCGG 4 cut(s) 344, 364, 497, 507
MspR9I CCNGG 4 cut(s) 269, 365, 498, 508
MvaI CCWGG 1 cut(s) 269
MvnI CGCG 1 cut(s) 188
MwoI GCNNNNNNNGC 4 cut(s) 306, 473, 482, 508
NciI CCSGG 3 cut(s) 365, 498, 508
NlaIII CATG 1 cut(s) 19
NlaIV GGNNCC 5 cut(s) 129, 130, 156, 286, 310
PaeR7I CTCGAG 1 cut(s) 330
PcsI WCGNNNNNNNCGW 1 cut(s) 146
PfeI GAWTC 2 cut(s) 178, 227
PflFI GACNNNGTC 1 cut(s) 269
PkrI GCNGC 1 cut(s) 475
PpuMI RGGWCCY 1 cut(s) 128
Psp5II RGGWCCY 1 cut(s) 128
Psp6I CCWGG 1 cut(s) 267
PspFI CCCAGC 2 cut(s) 203, 485
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 5 cut(s) 129, 130, 156, 286, 310
PspOMI GGGCCC 1 cut(s) 154
PspPI GGNCC 6 cut(s) 128, 154, 155, 494, 504, 509
PspPPI RGGWCCY 1 cut(s) 128
PstI CTGCAG 1 cut(s) 478
PsyI GACNNNGTC 1 cut(s) 269
RsaI GTAC 1 cut(s) 211
RsaNI GTAC 1 cut(s) 210
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 1 cut(s) 474
Sau96I GGNCC 6 cut(s) 128, 154, 155, 494, 504, 509
ScrFI CCNGG 4 cut(s) 269, 365, 498, 508
SduI GDGCHC 2 cut(s) 158, 492
SexAI ACCWGGT 1 cut(s) 267
SfcI CTRYAG 1 cut(s) 474
Sfr274I CTCGAG 1 cut(s) 330
SinI GGWCC 1 cut(s) 128
SlaI CTCGAG 1 cut(s) 330
SmlI CTYRAG 1 cut(s) 330
SmoI CTYRAG 1 cut(s) 330
SsiI CCGC 4 cut(s) 5, 158, 298, 502
StyD4I CCNGG 4 cut(s) 267, 363, 496, 506
StyI CCWWGG 1 cut(s) 276
TaiI ACGT 3 cut(s) 30, 226, 406
TaqI TCGA 1 cut(s) 331
TfiI GAWTC 2 cut(s) 178, 227
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TseI GCWGC 1 cut(s) 473
TspGWI ACGGA 1 cut(s) 234
Tth111I GACNNNGTC 1 cut(s) 269
VpaK11BI GGWCC 1 cut(s) 128
XhoI CTCGAG 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.