MD09G1010200.v1.1

50S ribosomal protein L27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009984
Physical Location & Seq
Reverse (-)
687735 .. 689232
1498 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1010200.v1.1.491

Sequence Viewer

Length: 612 bp
ATGGCGGTTGCGTCCATGGCTTTCAACGTCGTCGGAGCTTTCAGAGGGCTGTCTCTGCGCCCAACCTCCACCTCCACCTCCATCTCCAGCTCGTCCTCCTCGTCCTTCCTCAGAGGCGACTTGGGTTCTCTGCCAGTGGGTCCCAAGCTATCGTTAGTGTCGTCGGGCCCTCAGCGACTGCCATTGACGATTCAAAACGCGCACAAGAAAGGAGCCGGGAGTACAAAGAACGGCCGCGATTCCAAAGGGCAGAGGCTCGGCGTCAAGATTTACGGCGACCAGGTGGCCAAGGCTGGCTCCATCATTGTCCGCCAGCGCGGCACCAAGTTCCATGCAGGGAAGAATGTGGGGCTTGGCAAGGACCACACCATCTTCTCTTTGATCGACGGCATGGTGAAGTTCGAGAAGTTTGGACCCGACAAGAAGAAGATTAGTGTTTACCCGCGAGAAGTGCAGCCGGAGAACCCCAACAGCTACCGGGTTAAAAAGAGGGAGAACGTCAGGCTGCAGCGCGAACGTAAGAAAGCAAGGAAGGAAGGCTACATCCTCCAGAACAAACCCGAGCTAATACTTGCGTCTGCCGATGCTGCAGATGCTGATCCAGTCTGTTGA

Protein Analysis

204

Amino Acids

21.94

Weight (kDa)

10.38

Isoelectric Point (pI)

25.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27 PF01016 67 - 146 1.7e-35 Ribosomal L27 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 320
AccII CGCG 5 cut(s) 200, 237, 318, 445, 513
AciI CCGC 5 cut(s) 5, 235, 310, 318, 443
AclWI GGATC 1 cut(s) 593
AcoI YGGCCR 2 cut(s) 232, 285
AcyI GRCGYC 1 cut(s) 261
AfaI GTAC 1 cut(s) 223
AgsI TTSAA 2 cut(s) 25, 194
AjnI CCWGG 1 cut(s) 279
AluBI AGCT 5 cut(s) 38, 90, 148, 474, 565
AluI AGCT 5 cut(s) 38, 90, 148, 474, 565
Alw26I GTCTC 1 cut(s) 57
AlwI GGATC 1 cut(s) 593
AlwNI CAGNNNCTG 2 cut(s) 178, 596
Ama87I CYCGRG 1 cut(s) 560
AoxI GGCC 3 cut(s) 166, 232, 285
ApaI GGGCCC 1 cut(s) 170
ApeKI GCWGC 4 cut(s) 454, 505, 508, 587
AspLEI GCGC 4 cut(s) 60, 202, 318, 513
AspS9I GGNCC 5 cut(s) 140, 166, 167, 361, 413
AsuC2I CCSGG 2 cut(s) 217, 479
AsuHPI GGTGA 1 cut(s) 406
AvaI CYCGRG 1 cut(s) 560
AvaII GGWCC 3 cut(s) 140, 361, 413
BaeGI GKGCMC 1 cut(s) 170
BalI TGGCCA 1 cut(s) 287
BanI GGYRCC 1 cut(s) 320
BanII GRGCYC 1 cut(s) 170
BarI GAAGNNNNNNTAC 2 cut(s) 524, 556
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 4 cut(s) 466, 492, 520, 574
BccI CCATC 3 cut(s) 89, 308, 377
BceAI ACGGC 3 cut(s) 247, 289, 403
BciT130I CCWGG 1 cut(s) 281
BcnI CCSGG 2 cut(s) 217, 479
BcoDI GTCTC 1 cut(s) 57
BfmI CTRYAG 2 cut(s) 506, 588
BglI GCCNNNNNGGC 1 cut(s) 318
BisI GCNGC 6 cut(s) 235, 319, 455, 506, 509, 588
BlsI GCNGC 6 cut(s) 236, 320, 456, 507, 510, 589
Bme1390I CCNGG 3 cut(s) 217, 281, 479
Bme18I GGWCC 3 cut(s) 140, 361, 413
BmeT110I CYCGRG 1 cut(s) 560
BmgT120I GGNCC 5 cut(s) 140, 166, 167, 361, 413
BmiI GGNNCC 7 cut(s) 141, 142, 168, 214, 298, 322, 415
BmrFI CCNGG 3 cut(s) 217, 281, 479
BmsI GCATC 2 cut(s) 574, 583
BpmI CTGGAG 2 cut(s) 70, 533
Bpu10I CCTNAGC 1 cut(s) 171
BpuMI CCSGG 2 cut(s) 217, 479
BsaBI GATNNNNATC 1 cut(s) 597
BsaHI GRCGYC 1 cut(s) 261
BsaJI CCNNGG 2 cut(s) 15, 288
Bse1I ACTGG 2 cut(s) 134, 602
Bse8I GATNNNNATC 1 cut(s) 597
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 2 cut(s) 15, 288
BseGI GGATG 1 cut(s) 543
BseJI GATNNNNATC 1 cut(s) 597
BseMII CTCAG 2 cut(s) 124, 185
BseNI ACTGG 2 cut(s) 134, 602
BseRI GAGGAG 1 cut(s) 88
BseSI GKGCMC 1 cut(s) 170
BseX3I CGGCCG 1 cut(s) 232
BseXI GCAGC 4 cut(s) 466, 492, 520, 574
BsgI GTGCAG 1 cut(s) 473
Bsh1236I CGCG 5 cut(s) 200, 237, 318, 445, 513
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 3 cut(s) 168, 234, 287
BshNI GGYRCC 1 cut(s) 320
BsiEI CGRYCG 1 cut(s) 235
BsiHKCI CYCGRG 1 cut(s) 560
BsiSI CCGG 3 cut(s) 216, 458, 478
BslFI GGGAC 1 cut(s) 126
BsmAI GTCTC 1 cut(s) 57
BsmFI GGGAC 1 cut(s) 126
BsnI GGCC 3 cut(s) 168, 234, 287
BsoBI CYCGRG 1 cut(s) 560
Bsp120I GGGCCC 1 cut(s) 166
Bsp1286I GDGCHC 1 cut(s) 170
Bsp143I GATC 2 cut(s) 381, 598
Bsp19I CCATGG 1 cut(s) 15
BspACI CCGC 5 cut(s) 5, 235, 310, 318, 443
BspANI GGCC 3 cut(s) 168, 234, 287
BspCNI CTCAG 2 cut(s) 123, 184
BspFNI CGCG 5 cut(s) 200, 237, 318, 445, 513
BspLI GGNNCC 7 cut(s) 141, 142, 168, 214, 298, 322, 415
BspMAI CTGCAG 2 cut(s) 510, 592
BspPI GGATC 1 cut(s) 593
BspT107I GGYRCC 1 cut(s) 320
BsrI ACTGG 2 cut(s) 134, 602
BssECI CCNNGG 2 cut(s) 15, 288
BssMI GATC 2 cut(s) 381, 598
BssNI GRCGYC 1 cut(s) 261
BssT1I CCWWGG 2 cut(s) 15, 288
Bst2UI CCWGG 1 cut(s) 281
BstACI GRCGYC 1 cut(s) 261
BstC8I GCNNGC 2 cut(s) 295, 314
BstDEI CTNAG 2 cut(s) 110, 171
BstDSI CCRYGG 1 cut(s) 15
BstF5I GGATG 1 cut(s) 543
BstFNI CGCG 5 cut(s) 200, 237, 318, 445, 513
BstHHI GCGC 4 cut(s) 60, 202, 318, 513
BstKTI GATC 2 cut(s) 384, 601
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 2 cut(s) 381, 598
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 6 cut(s) 17, 55, 318, 451, 587, 593
BstNI CCWGG 1 cut(s) 281
BstSCI CCNGG 3 cut(s) 215, 279, 477
BstSFI CTRYAG 2 cut(s) 506, 588
BstSLI GKGCMC 1 cut(s) 170
BstUI CGCG 5 cut(s) 200, 237, 318, 445, 513
BstV1I GCAGC 4 cut(s) 466, 492, 520, 574
BstZI CGGCCG 1 cut(s) 232
BsuRI GGCC 3 cut(s) 168, 234, 287
BtgI CCRYGG 1 cut(s) 15
BtsCI GGATG 1 cut(s) 543
BtsIMutI CAGTG 1 cut(s) 141
Cac8I GCNNGC 2 cut(s) 295, 314
CaiI CAGNNNCTG 2 cut(s) 178, 596
CfoI GCGC 4 cut(s) 60, 202, 318, 513
Cfr13I GGNCC 5 cut(s) 140, 166, 167, 361, 413
CseI GACGC 2 cut(s) 250, 564
CsiI ACCWGGT 1 cut(s) 279
Csp6I GTAC 1 cut(s) 222
CviAII CATG 3 cut(s) 16, 332, 391
CviQI GTAC 1 cut(s) 222
DdeI CTNAG 2 cut(s) 110, 171
DpnI GATC 2 cut(s) 383, 600
DpnII GATC 2 cut(s) 381, 598
EaeI YGGCCR 2 cut(s) 232, 285
EagI CGGCCG 1 cut(s) 232
EciI GGCGGA 1 cut(s) 299
EclXI CGGCCG 1 cut(s) 232
Eco130I CCWWGG 2 cut(s) 15, 288
Eco24I GRGCYC 1 cut(s) 170
Eco47I GGWCC 3 cut(s) 140, 361, 413
Eco52I CGGCCG 1 cut(s) 232
Eco88I CYCGRG 1 cut(s) 560
EcoO109I RGGNCCY 2 cut(s) 140, 167
EcoRII CCWGG 1 cut(s) 279
EcoT14I CCWWGG 2 cut(s) 15, 288
EcoT38I GRGCYC 1 cut(s) 170
ErhI CCWWGG 2 cut(s) 15, 288
FaeI CATG 3 cut(s) 19, 335, 394
FaiI YATR 3 cut(s) 17, 333, 392
FaqI GGGAC 1 cut(s) 126
FatI CATG 3 cut(s) 15, 331, 390
FauI CCCGC 1 cut(s) 450
Fnu4HI GCNGC 6 cut(s) 235, 319, 455, 506, 509, 588
FokI GGATG 1 cut(s) 530
FriOI GRGCYC 1 cut(s) 170
Fsp4HI GCNGC 6 cut(s) 235, 319, 455, 506, 509, 588
GlaI GCGC 4 cut(s) 59, 201, 317, 512
GluI GCNGC 6 cut(s) 235, 319, 455, 506, 509, 588
GsuI CTGGAG 2 cut(s) 70, 533
HaeIII GGCC 3 cut(s) 168, 234, 287
HapII CCGG 3 cut(s) 216, 458, 478
HgaI GACGC 2 cut(s) 250, 564
HhaI GCGC 4 cut(s) 60, 202, 318, 513
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 3 cut(s) 19, 335, 394
Hin6I GCGC 4 cut(s) 58, 200, 316, 511
HinP1I GCGC 4 cut(s) 58, 200, 316, 511
HinfI GANTC 2 cut(s) 190, 239
HpaII CCGG 3 cut(s) 216, 458, 478
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 1 cut(s) 439
Hpy188I TCNGA 3 cut(s) 35, 44, 113
Hpy188III TCNNGA 3 cut(s) 265, 403, 550
Hpy8I GTNNAC 1 cut(s) 439
Hpy99I CGWCG 4 cut(s) 32, 35, 166, 389
HpyAV CCTTC 3 cut(s) 115, 526, 530
HpyCH4IV ACGT 3 cut(s) 27, 498, 517
HpyCH4V TGCA 4 cut(s) 335, 454, 508, 590
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 55, 318, 451, 587, 593
HpyF3I CTNAG 2 cut(s) 110, 171
HpySE526I ACGT 3 cut(s) 27, 498, 517
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 3 cut(s) 19, 335, 394
HspAI GCGC 4 cut(s) 58, 200, 316, 511
KflI GGGWCCC 1 cut(s) 140
Kzo9I GATC 2 cut(s) 381, 598
LmnI GCTCC 3 cut(s) 35, 212, 302
Lsp1109I GCAGC 4 cut(s) 466, 492, 520, 574
LweI GCATC 2 cut(s) 574, 583
MabI ACCWGGT 1 cut(s) 279
MaeII ACGT 3 cut(s) 27, 498, 517
MalI GATC 2 cut(s) 383, 600
MboI GATC 2 cut(s) 381, 598
MboII GAAGA 4 cut(s) 352, 364, 436, 439
MhlI GDGCHC 1 cut(s) 170
MlsI TGGCCA 1 cut(s) 287
MluNI TGGCCA 1 cut(s) 287
MmeI TCCRAC 1 cut(s) 13
Mox20I TGGCCA 1 cut(s) 287
MscI TGGCCA 1 cut(s) 287
MseI TTAA 1 cut(s) 483
Msp20I TGGCCA 1 cut(s) 287
MspI CCGG 3 cut(s) 216, 458, 478
MspR9I CCNGG 3 cut(s) 217, 281, 479
MvaI CCWGG 1 cut(s) 281
MvnI CGCG 5 cut(s) 200, 237, 318, 445, 513
MwoI GCNNNNNNNGC 6 cut(s) 17, 55, 318, 451, 587, 593
NciI CCSGG 2 cut(s) 217, 479
NcoI CCATGG 1 cut(s) 15
NdeII GATC 2 cut(s) 381, 598
NlaIII CATG 3 cut(s) 19, 335, 394
NlaIV GGNNCC 7 cut(s) 141, 142, 168, 214, 298, 322, 415
NmeAIII GCCGAG 1 cut(s) 237
PcsI WCGNNNNNNNCGW 2 cut(s) 98, 158
PfeI GAWTC 2 cut(s) 190, 239
PkrI GCNGC 6 cut(s) 236, 320, 456, 507, 510, 589
PpuMI RGGWCCY 1 cut(s) 140
Psp5II RGGWCCY 1 cut(s) 140
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PspN4I GGNNCC 7 cut(s) 141, 142, 168, 214, 298, 322, 415
PspOMI GGGCCC 1 cut(s) 166
PspPI GGNCC 5 cut(s) 140, 166, 167, 361, 413
PspPPI RGGWCCY 1 cut(s) 140
PstI CTGCAG 2 cut(s) 510, 592
PstNI CAGNNNCTG 2 cut(s) 178, 596
RsaI GTAC 1 cut(s) 223
RsaNI GTAC 1 cut(s) 222
SaqAI TTAA 1 cut(s) 483
SatI GCNGC 6 cut(s) 235, 319, 455, 506, 509, 588
Sau3AI GATC 2 cut(s) 381, 598
Sau96I GGNCC 5 cut(s) 140, 166, 167, 361, 413
ScrFI CCNGG 3 cut(s) 217, 281, 479
SduI GDGCHC 1 cut(s) 170
SexAI ACCWGGT 1 cut(s) 279
SfaNI GCATC 2 cut(s) 574, 583
SfcI CTRYAG 2 cut(s) 506, 588
SinI GGWCC 3 cut(s) 140, 361, 413
SsiI CCGC 5 cut(s) 5, 235, 310, 318, 443
StyD4I CCNGG 3 cut(s) 215, 279, 477
StyI CCWWGG 2 cut(s) 15, 288
TaiI ACGT 3 cut(s) 30, 501, 520
TaqI TCGA 2 cut(s) 384, 402
TatI WGTACW 1 cut(s) 221
TauI GCSGC 2 cut(s) 237, 321
TfiI GAWTC 2 cut(s) 190, 239
Tru1I TTAA 1 cut(s) 483
Tru9I TTAA 1 cut(s) 483
TscAI CASTG 1 cut(s) 141
TseI GCWGC 4 cut(s) 454, 505, 508, 587
TspRI CASTG 1 cut(s) 141
VpaK11BI GGWCC 3 cut(s) 140, 361, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.