Rorug02G0587100

50S ribosomal protein L27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009984
Physical Location & Seq
Reverse (-)
70999055 .. 70999864
810 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0587100.1

Sequence Viewer

Length: 810 bp
ATGGATGCTCAACAGAAGATTTATGTGGAGTGTTTGCCATGGGACAAAGCTTGGAACTTATTTCAGGAGAAGGTTGGCAAAGACGCACTTCTTATACATGCAGATATTCCTAAACTTGCTGAAGCTGTCGCAAAAGAGTGTGGTGGTTTGCCGCTTGCACTAATTACAGTTGGTCGGATGATGTCTTGCAAGAAGACACCCCAAGAATGGAATCATGCGATCGAAGTATTGAAGAAATTTAGCTTCGAGTATTCAGGTATGGGAGACCAGGTGTTCCCCCTCTTGAAATTCAGTTACGATAATTTTCCTAGTGAGAAAGTCAGATCATGCTTCTTGTACTGTGCTTTATTTCCTGAAGACTATCCCATACCTAAAGATGATTTAGCATGCTTTTGGATGTGTGAAAATATGTTAGATGAACATACTGATCTAGAAGAAGCACGAGATGAGAGCTACCATATCATAGGTACTCTTCTCAATGCATGTATGCTAGAAGAGAGTAAAGAAGGTTGTGTAAAAATGCATGATGTTGTTCGTGACATGGCATTGTGGTTGGCTTGTGATCCGAAGAAAGCAGATGAGAGTTTTCTTGTGCATGCAGGCGCTGATTTGATTGAAGCACCAATTGCCGAAAAATGGAAGAACTCGAAAAGGGTATCATTGATGGCCAACCACATCAAAGAATTGGTTGAAAAACCAAACTCTCCATATCTGTTGACTTTGTTTCTCACGGGAAATCATCTGAAGATTATTGTTGCAGGCTTCTTTGCCTCTATGTCTAAGCTGCTAGTTTTAGATCTCAAATCATGA

Protein Analysis

269

Amino Acids

30.57

Weight (kDa)

5.33

Isoelectric Point (pI)

51.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 116 - 182 2.7e-09 Disease resistance protein Winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 152
AclWI GGATC 1 cut(s) 557
AcoI YGGCCR 1 cut(s) 666
AcsI RAATTY 2 cut(s) 236, 287
AcuI CTGAAG 3 cut(s) 141, 375, 764
AfaI GTAC 2 cut(s) 338, 469
AfiI CCNNNNNNNGG 2 cut(s) 207, 636
AgsI TTSAA 4 cut(s) 232, 286, 617, 692
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 5 cut(s) 50, 125, 243, 453, 784
AluI AGCT 5 cut(s) 50, 125, 243, 453, 784
Alw26I GTCTC 1 cut(s) 258
AlwI GGATC 1 cut(s) 557
AlwNI CAGNNNCTG 1 cut(s) 605
AoxI GGCC 1 cut(s) 666
ApeKI GCWGC 1 cut(s) 784
ApoI RAATTY 2 cut(s) 236, 287
AspLEI GCGC 1 cut(s) 605
BalI TGGCCA 1 cut(s) 668
BauI CACGAG 1 cut(s) 441
BbsI GAAGAC 2 cut(s) 200, 363
BbvI GCAGC 1 cut(s) 771
BccI CCATC 1 cut(s) 658
BciT130I CCWGG 1 cut(s) 269
BcoDI GTCTC 1 cut(s) 258
BfaI CTAG 4 cut(s) 309, 431, 491, 788
BfoI RGCGCY 1 cut(s) 606
BglII AGATCT 1 cut(s) 796
BisI GCNGC 2 cut(s) 152, 785
BlsI GCNGC 2 cut(s) 153, 786
Bme1390I CCNGG 1 cut(s) 269
BmrFI CCNGG 1 cut(s) 269
BpiI GAAGAC 2 cut(s) 200, 363
BsaI GGTCTC 1 cut(s) 258
BsaJI CCNNGG 1 cut(s) 38
BsaXI ACNNNNNCTCC 2 cut(s) 255, 285
Bsc4I CCNNNNNNNGG 2 cut(s) 207, 636
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 1 cut(s) 38
BseGI GGATG 3 cut(s) 10, 183, 402
BseLI CCNNNNNNNGG 2 cut(s) 207, 636
BseXI GCAGC 1 cut(s) 771
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 1 cut(s) 668
BsiEI CGRYCG 1 cut(s) 222
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 2 cut(s) 207, 636
BsmAI GTCTC 1 cut(s) 258
BsmFI GGGAC 1 cut(s) 56
BsnI GGCC 1 cut(s) 668
Bso31I GGTCTC 1 cut(s) 258
Bsp143I GATC 5 cut(s) 219, 323, 427, 562, 796
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 1 cut(s) 152
BspANI GGCC 1 cut(s) 668
BspHI TCATGA 1 cut(s) 806
BspPI GGATC 1 cut(s) 557
BspTNI GGTCTC 1 cut(s) 258
BssECI CCNNGG 1 cut(s) 38
BssMI GATC 5 cut(s) 219, 323, 427, 562, 796
BssSI CACGAG 1 cut(s) 441
BssT1I CCWWGG 1 cut(s) 38
Bst2BI CACGAG 1 cut(s) 441
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 2 cut(s) 169, 341
Bst6I CTCTTC 2 cut(s) 477, 489
BstAPI GCANNNNNTGC 1 cut(s) 626
BstC8I GCNNGC 5 cut(s) 156, 388, 597, 601, 760
BstDEI CTNAG 1 cut(s) 780
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 3 cut(s) 10, 183, 402
BstH2I RGCGCY 1 cut(s) 606
BstHHI GCGC 1 cut(s) 605
BstKTI GATC 5 cut(s) 222, 326, 430, 565, 799
BstMAI GTCTC 1 cut(s) 258
BstMBI GATC 5 cut(s) 219, 323, 427, 562, 796
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 1 cut(s) 626
BstNI CCWGG 1 cut(s) 269
BstNSI RCATGY 4 cut(s) 101, 390, 486, 599
BstSCI CCNGG 1 cut(s) 267
BstV1I GCAGC 1 cut(s) 771
BstV2I GAAGAC 2 cut(s) 200, 363
BstX2I RGATCY 1 cut(s) 796
BstYI RGATCY 1 cut(s) 796
BsuRI GGCC 1 cut(s) 668
BtgI CCRYGG 1 cut(s) 38
BtsCI GGATG 3 cut(s) 10, 183, 402
Cac8I GCNNGC 5 cut(s) 156, 388, 597, 601, 760
CaiI CAGNNNCTG 1 cut(s) 605
CciI TCATGA 1 cut(s) 806
CfoI GCGC 1 cut(s) 605
CseI GACGC 1 cut(s) 92
CsiI ACCWGGT 1 cut(s) 267
Csp6I GTAC 2 cut(s) 337, 468
CviJI RGCY 8 cut(s) 50, 125, 243, 453, 557, 668, 762, 784
CviKI_1 RGCY 8 cut(s) 50, 125, 243, 453, 557, 668, 762, 784
CviQI GTAC 2 cut(s) 337, 468
DdeI CTNAG 1 cut(s) 780
DpnI GATC 5 cut(s) 221, 325, 429, 564, 798
DpnII GATC 5 cut(s) 219, 323, 427, 562, 796
EaeI YGGCCR 1 cut(s) 666
Eam1104I CTCTTC 2 cut(s) 477, 489
EarI CTCTTC 2 cut(s) 477, 489
Eco130I CCWWGG 1 cut(s) 38
Eco31I GGTCTC 1 cut(s) 258
Eco57I CTGAAG 3 cut(s) 141, 375, 764
EcoRII CCWGG 1 cut(s) 267
EcoT14I CCWWGG 1 cut(s) 38
EcoT22I ATGCAT 2 cut(s) 484, 525
ErhI CCWWGG 1 cut(s) 38
FalI AAGNNNNNCTT 2 cut(s) 72, 104
FaqI GGGAC 1 cut(s) 56
Fnu4HI GCNGC 2 cut(s) 152, 785
FokI GGATG 3 cut(s) 17, 190, 409
Fsp4HI GCNGC 2 cut(s) 152, 785
FspBI CTAG 4 cut(s) 309, 431, 491, 788
GlaI GCGC 1 cut(s) 604
GluI GCNGC 2 cut(s) 152, 785
HaeII RGCGCY 1 cut(s) 606
HaeIII GGCC 1 cut(s) 668
HgaI GACGC 1 cut(s) 92
HhaI GCGC 1 cut(s) 605
Hin6I GCGC 1 cut(s) 603
HinP1I GCGC 1 cut(s) 603
HincII GTYRAC 1 cut(s) 717
HindII GTYRAC 1 cut(s) 717
HindIII AAGCTT 1 cut(s) 48
HinfI GANTC 1 cut(s) 211
Hpy166II GTNNAC 1 cut(s) 717
Hpy188I TCNGA 4 cut(s) 177, 323, 567, 744
Hpy188III TCNNGA 6 cut(s) 65, 283, 353, 431, 536, 807
Hpy8I GTNNAC 1 cut(s) 717
HpyAV CCTTC 2 cut(s) 64, 500
HpyCH4III ACNGT 2 cut(s) 169, 341
HpyCH4V TGCA 8 cut(s) 101, 158, 189, 482, 523, 595, 599, 758
HpyF10VI GCNNNNNNNGC 1 cut(s) 626
HpyF3I CTNAG 1 cut(s) 780
HspAI GCGC 1 cut(s) 603
Kzo9I GATC 5 cut(s) 219, 323, 427, 562, 796
LpnPI CCDG 7 cut(s) 50, 240, 254, 281, 366, 585, 744
Lsp1109I GCAGC 1 cut(s) 771
MabI ACCWGGT 1 cut(s) 267
MaeI CTAG 4 cut(s) 309, 431, 491, 788
MaeIII GTNAC 2 cut(s) 293, 536
MalI GATC 5 cut(s) 221, 325, 429, 564, 798
MboI GATC 5 cut(s) 219, 323, 427, 562, 796
MfeI CAATTG 1 cut(s) 624
MflI RGATCY 1 cut(s) 796
MlsI TGGCCA 1 cut(s) 668
MluCI AATT 6 cut(s) 162, 236, 287, 301, 624, 683
MluNI TGGCCA 1 cut(s) 668
MmeI TCCRAC 1 cut(s) 155
MnlI CCTC 2 cut(s) 290, 781
Mox20I TGGCCA 1 cut(s) 668
Mph1103I ATGCAT 2 cut(s) 484, 525
MscI TGGCCA 1 cut(s) 668
Msp20I TGGCCA 1 cut(s) 668
MspR9I CCNGG 1 cut(s) 269
MunI CAATTG 1 cut(s) 624
MvaI CCWGG 1 cut(s) 269
MwoI GCNNNNNNNGC 1 cut(s) 626
NcoI CCATGG 1 cut(s) 38
NdeII GATC 5 cut(s) 219, 323, 427, 562, 796
NmuCI GTSAC 1 cut(s) 536
NsiI ATGCAT 2 cut(s) 484, 525
NspI RCATGY 4 cut(s) 101, 390, 486, 599
PaeI GCATGC 2 cut(s) 390, 599
PagI TCATGA 1 cut(s) 806
PfeI GAWTC 1 cut(s) 211
PkrI GCNGC 2 cut(s) 153, 786
Ple19I CGATCG 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 267
PspGI CCWGG 1 cut(s) 267
PstNI CAGNNNCTG 1 cut(s) 605
PsuI RGATCY 1 cut(s) 796
PvuI CGATCG 1 cut(s) 222
RsaI GTAC 2 cut(s) 338, 469
RsaNI GTAC 2 cut(s) 337, 468
SatI GCNGC 2 cut(s) 152, 785
Sau3AI GATC 5 cut(s) 219, 323, 427, 562, 796
ScrFI CCNGG 1 cut(s) 269
SexAI ACCWGGT 1 cut(s) 267
SphI GCATGC 2 cut(s) 390, 599
Sse9I AATT 6 cut(s) 162, 236, 287, 301, 624, 683
SsiI CCGC 1 cut(s) 152
SspMI CTAG 4 cut(s) 309, 431, 491, 788
StyD4I CCNGG 1 cut(s) 267
StyI CCWWGG 1 cut(s) 38
TaaI ACNGT 2 cut(s) 169, 341
TaqI TCGA 3 cut(s) 222, 246, 647
TasI AATT 6 cut(s) 162, 236, 287, 301, 624, 683
TatI WGTACW 1 cut(s) 336
TauI GCSGC 1 cut(s) 154
TfiI GAWTC 1 cut(s) 211
TseFI GTSAC 1 cut(s) 536
TseI GCWGC 1 cut(s) 784
Tsp45I GTSAC 1 cut(s) 536
TspDTI ATGAA 1 cut(s) 432
XapI RAATTY 2 cut(s) 236, 287
XbaI TCTAGA 1 cut(s) 430
XceI RCATGY 4 cut(s) 101, 390, 486, 599
XspI CTAG 4 cut(s) 309, 431, 491, 788
Zsp2I ATGCAT 2 cut(s) 484, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.