RLG00000022346

50S ribosomal protein L27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009984
Physical Location & Seq
Forward (+)
82735896 .. 82737015
1120 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022346

Sequence Viewer

Length: 594 bp
ATGGCGGTCGCGTCTATGAGTTTCAACCTAGTCGGAGCATTCAGAGGGATGGCTCTGAGCTCAAGCTCTTCCTCTTCCTCCTCTTTCTTCAGAGGTGATTTGGGCTCCCTACAGTTGGGACCAAAGCTATCCTTGTCTACTACTACCCAACGCAGATTGCCATTGACGATTCAAAACGCGCACAAGAAAGGAGCTGGTAGTACCAAGAACGGTCGCGATTCCAAAGGCCAAAGGCTCGGGGTTAAGATTTACGGAGACCAGGTCGCCAAGCCCGGCTCCATCATCATACGTCAGCGGGGCACCAAGTTCCATGCGGGGAAGAATGTGGGGCTTGGCAAGGACCATACCATTTTCTCTCTGATTGATGGGTTGGTTAAGTTTGAGAAGTATGGACCTGACAGGAAGAAGGTGAGTGTTTACCCTCGGGAGATACAGCCTGAAAATCCCAACAGCTACAGGGTTAGGAAGAGGGAGAACTTCAAGCTACAACGCGAGAAAAAGAAAGCAAGAACGGAAGGCTATATTCTTCAAAACAAACCACAACTGGTGCTTGCATCTGCTGATAATGGTGCAGAGACCAATCCTCTTTGTTGA

Protein Analysis

198

Amino Acids

21.61

Weight (kDa)

10.46

Isoelectric Point (pI)

24.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27 PF01016 60 - 139 8.6e-36 Ribosomal L27 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 137
AccII CGCG 4 cut(s) 11, 179, 216, 492
AciI CCGC 3 cut(s) 5, 295, 314
AcuI CTGAAG 1 cut(s) 73
AfaI GTAC 1 cut(s) 202
AfiI CCNNNNNNNGG 1 cut(s) 115
AgsI TTSAA 4 cut(s) 25, 173, 481, 530
AjnI CCWGG 1 cut(s) 258
AluBI AGCT 6 cut(s) 60, 66, 127, 194, 453, 484
AluI AGCT 6 cut(s) 60, 66, 127, 194, 453, 484
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 2 cut(s) 249, 569
Ama87I CYCGRG 2 cut(s) 236, 423
AoxI GGCC 1 cut(s) 226
AspLEI GCGC 1 cut(s) 181
AspS9I GGNCC 3 cut(s) 119, 340, 392
AsuC2I CCSGG 1 cut(s) 273
AsuHPI GGTGA 2 cut(s) 107, 421
AvaI CYCGRG 2 cut(s) 236, 423
AvaII GGWCC 3 cut(s) 119, 340, 392
BaeGI GKGCMC 1 cut(s) 302
BanI GGYRCC 1 cut(s) 299
BanII GRGCYC 2 cut(s) 62, 107
Bbv12I GWGCWC 1 cut(s) 62
BccI CCATC 3 cut(s) 43, 287, 359
BciT130I CCWGG 1 cut(s) 260
BcnI CCSGG 1 cut(s) 273
BcoDI GTCTC 2 cut(s) 249, 569
BfaI CTAG 1 cut(s) 29
BfmI CTRYAG 2 cut(s) 110, 454
Bme1390I CCNGG 2 cut(s) 260, 273
Bme18I GGWCC 3 cut(s) 119, 340, 392
BmeT110I CYCGRG 2 cut(s) 236, 423
BmgT120I GGNCC 3 cut(s) 119, 340, 392
BmiI GGNNCC 4 cut(s) 106, 120, 277, 301
BmrFI CCNGG 2 cut(s) 260, 273
BmsI GCATC 1 cut(s) 563
BpuEI CTTGAG 1 cut(s) 46
BpuMI CCSGG 1 cut(s) 273
BsaI GGTCTC 2 cut(s) 249, 569
BsaJI CCNNGG 1 cut(s) 422
BsaXI ACNNNNNCTCC 2 cut(s) 246, 276
Bsc4I CCNNNNNNNGG 1 cut(s) 115
Bse1I ACTGG 1 cut(s) 549
BseBI CCWGG 1 cut(s) 260
BseDI CCNNGG 1 cut(s) 422
BseGI GGATG 1 cut(s) 54
BseLI CCNNNNNNNGG 1 cut(s) 115
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 549
BseRI GAGGAG 1 cut(s) 70
BseSI GKGCMC 1 cut(s) 302
BsgI GTGCAG 1 cut(s) 591
Bsh1236I CGCG 4 cut(s) 11, 179, 216, 492
Bsh1285I CGRYCG 2 cut(s) 9, 214
BshFI GGCC 1 cut(s) 228
BshNI GGYRCC 1 cut(s) 299
BsiEI CGRYCG 2 cut(s) 9, 214
BsiHKAI GWGCWC 1 cut(s) 62
BsiHKCI CYCGRG 2 cut(s) 236, 423
BsiSI CCGG 1 cut(s) 273
BslFI GGGAC 1 cut(s) 132
BslI CCNNNNNNNGG 1 cut(s) 115
BsmAI GTCTC 2 cut(s) 249, 569
BsmFI GGGAC 1 cut(s) 132
BsmI GAATGC 1 cut(s) 38
BsnI GGCC 1 cut(s) 228
Bso31I GGTCTC 2 cut(s) 249, 569
BsoBI CYCGRG 2 cut(s) 236, 423
Bsp1286I GDGCHC 3 cut(s) 62, 107, 302
Bsp68I TCGCGA 1 cut(s) 216
BspACI CCGC 3 cut(s) 5, 295, 314
BspANI GGCC 1 cut(s) 228
BspCNI CTCAG 1 cut(s) 48
BspFNI CGCG 4 cut(s) 11, 179, 216, 492
BspLI GGNNCC 4 cut(s) 106, 120, 277, 301
BspQI GCTCTTC 1 cut(s) 73
BspT107I GGYRCC 1 cut(s) 299
BspTNI GGTCTC 2 cut(s) 249, 569
BsrI ACTGG 1 cut(s) 549
BssECI CCNNGG 1 cut(s) 422
Bst2UI CCWGG 1 cut(s) 260
Bst4CI ACNGT 2 cut(s) 114, 212
Bst6I CTCTTC 3 cut(s) 73, 79, 461
BstC8I GCNNGC 1 cut(s) 552
BstDEI CTNAG 1 cut(s) 56
BstF5I GGATG 1 cut(s) 54
BstFNI CGCG 4 cut(s) 11, 179, 216, 492
BstHHI GCGC 1 cut(s) 181
BstMAI GTCTC 2 cut(s) 249, 569
BstMCI CGRYCG 2 cut(s) 9, 214
BstNI CCWGG 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 258, 271
BstSFI CTRYAG 2 cut(s) 110, 454
BstSLI GKGCMC 1 cut(s) 302
BstUI CGCG 4 cut(s) 11, 179, 216, 492
BsuRI GGCC 1 cut(s) 228
BtsCI GGATG 1 cut(s) 54
BtuMI TCGCGA 1 cut(s) 216
Cac8I GCNNGC 1 cut(s) 552
CfoI GCGC 1 cut(s) 181
Cfr13I GGNCC 3 cut(s) 119, 340, 392
CsiI ACCWGGT 1 cut(s) 258
Csp6I GTAC 1 cut(s) 201
CviAII CATG 1 cut(s) 311
CviQI GTAC 1 cut(s) 201
DdeI CTNAG 1 cut(s) 56
Eam1104I CTCTTC 3 cut(s) 73, 79, 461
EarI CTCTTC 3 cut(s) 73, 79, 461
Ecl136II GAGCTC 1 cut(s) 60
Eco24I GRGCYC 2 cut(s) 62, 107
Eco31I GGTCTC 2 cut(s) 249, 569
Eco47I GGWCC 3 cut(s) 119, 340, 392
Eco53kI GAGCTC 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 73
Eco88I CYCGRG 2 cut(s) 236, 423
EcoICRI GAGCTC 1 cut(s) 60
EcoRII CCWGG 1 cut(s) 258
EcoT38I GRGCYC 2 cut(s) 62, 107
FaeI CATG 1 cut(s) 314
FaiI YATR 6 cut(s) 17, 287, 312, 345, 390, 522
FalI AAGNNNNNCTT 2 cut(s) 116, 148
FaqI GGGAC 1 cut(s) 132
FatI CATG 1 cut(s) 310
FauI CCCGC 2 cut(s) 288, 307
FblI GTMKAC 1 cut(s) 137
FokI GGATG 1 cut(s) 61
FriOI GRGCYC 2 cut(s) 62, 107
FspBI CTAG 1 cut(s) 29
GlaI GCGC 1 cut(s) 180
HaeIII GGCC 1 cut(s) 228
HapII CCGG 1 cut(s) 273
HhaI GCGC 1 cut(s) 181
Hin1II CATG 1 cut(s) 314
Hin6I GCGC 1 cut(s) 179
HinP1I GCGC 1 cut(s) 179
HinfI GANTC 2 cut(s) 169, 218
HpaII CCGG 1 cut(s) 273
HphI GGTGA 2 cut(s) 107, 421
Hpy166II GTNNAC 2 cut(s) 138, 418
Hpy188I TCNGA 5 cut(s) 35, 44, 57, 92, 360
Hpy188III TCNNGA 2 cut(s) 215, 425
Hpy8I GTNNAC 2 cut(s) 138, 418
HpyAV CCTTC 2 cut(s) 400, 509
HpyCH4III ACNGT 2 cut(s) 114, 212
HpyCH4IV ACGT 1 cut(s) 289
HpyCH4V TGCA 2 cut(s) 554, 572
HpyF3I CTNAG 1 cut(s) 56
HpySE526I ACGT 1 cut(s) 289
Hsp92II CATG 1 cut(s) 314
HspAI GCGC 1 cut(s) 179
LguI GCTCTTC 1 cut(s) 73
LmnI GCTCC 4 cut(s) 35, 110, 191, 281
LpnPI CCDG 9 cut(s) 180, 245, 272, 286, 385, 408, 442, 450, 530
LweI GCATC 1 cut(s) 563
MabI ACCWGGT 1 cut(s) 258
MaeI CTAG 1 cut(s) 29
MaeII ACGT 1 cut(s) 289
MboII GAAGA 7 cut(s) 60, 66, 79, 331, 415, 478, 518
MhlI GDGCHC 3 cut(s) 62, 107, 302
MmeI TCCRAC 1 cut(s) 13
MnlI CCTC 8 cut(s) 38, 82, 86, 88, 91, 432, 462, 594
MseI TTAA 2 cut(s) 243, 375
MspA1I CMGCKG 1 cut(s) 295
MspI CCGG 1 cut(s) 273
MspR9I CCNGG 2 cut(s) 260, 273
Mva1269I GAATGC 1 cut(s) 38
MvaI CCWGG 1 cut(s) 260
MvnI CGCG 4 cut(s) 11, 179, 216, 492
NciI CCSGG 1 cut(s) 273
NlaIII CATG 1 cut(s) 314
NlaIV GGNNCC 4 cut(s) 106, 120, 277, 301
NruI TCGCGA 1 cut(s) 216
PciSI GCTCTTC 1 cut(s) 73
PctI GAATGC 1 cut(s) 38
PfeI GAWTC 2 cut(s) 169, 218
PflFI GACNNNGTC 1 cut(s) 260
Psp124BI GAGCTC 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 258
PspGI CCWGG 1 cut(s) 258
PspN4I GGNNCC 4 cut(s) 106, 120, 277, 301
PspPI GGNCC 3 cut(s) 119, 340, 392
PsyI GACNNNGTC 1 cut(s) 260
RruI TCGCGA 1 cut(s) 216
RsaI GTAC 1 cut(s) 202
RsaNI GTAC 1 cut(s) 201
SacI GAGCTC 1 cut(s) 62
SapI GCTCTTC 1 cut(s) 73
SaqAI TTAA 2 cut(s) 243, 375
Sau96I GGNCC 3 cut(s) 119, 340, 392
ScrFI CCNGG 2 cut(s) 260, 273
SduI GDGCHC 3 cut(s) 62, 107, 302
SexAI ACCWGGT 1 cut(s) 258
SfaNI GCATC 1 cut(s) 563
SfcI CTRYAG 2 cut(s) 110, 454
SinI GGWCC 3 cut(s) 119, 340, 392
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
SsiI CCGC 3 cut(s) 5, 295, 314
SspMI CTAG 1 cut(s) 29
SstI GAGCTC 1 cut(s) 62
StyD4I CCNGG 2 cut(s) 258, 271
TaaI ACNGT 2 cut(s) 114, 212
TaiI ACGT 1 cut(s) 292
TfiI GAWTC 2 cut(s) 169, 218
Tru1I TTAA 2 cut(s) 243, 375
Tru9I TTAA 2 cut(s) 243, 375
TspGWI ACGGA 2 cut(s) 267, 527
Tth111I GACNNNGTC 1 cut(s) 260
VpaK11BI GGWCC 3 cut(s) 119, 340, 392
XmiI GTMKAC 1 cut(s) 137
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.