Rorug02G0586900

50S ribosomal protein L27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009984
Physical Location & Seq
Forward (+)
70995839 .. 70996279
441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0586900.1

Sequence Viewer

Length: 333 bp
ATGATAAATGCTACTTCTCAGTCACAACAATCAAGTCGACCAATCAATGCTCCAAGCAATGATCTTGAAAATAGAAGATGTGCACTTTTGAATTGGAACAATTATGAAGAAGATGAAGTGGTGGCTGAAGGGAAGATCTCTTCAACAAATCCAAGTACAAAAGTTCATTGTGTGCCTCTTGGACGAGATTGTTGGAAGGTTTGGGTTGATGTGGTGTTTGATGAGTATCAGACTATGGAAGTGTATAGAGCAACCATGGATGCCAAACAACTTGGTGAAGCTGTAGGAAGCACTCTTGCATGGCCCAAAAGCTCCATTAAAATGCTCCCTTAG

Protein Analysis

110

Amino Acids

12.38

Weight (kDa)

5.07

Isoelectric Point (pI)

46.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8039 PF26133 24 - 108 2.7e-15 Domain of unknown function (DUF8039)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 37
AcuI CTGAAG 1 cut(s) 147
AfaI GTAC 1 cut(s) 157
AgsI TTSAA 3 cut(s) 68, 91, 144
AluBI AGCT 2 cut(s) 281, 312
AluI AGCT 2 cut(s) 281, 312
Alw21I GWGCWC 1 cut(s) 85
Alw44I GTGCAC 1 cut(s) 81
AoxI GGCC 1 cut(s) 302
ApaLI GTGCAC 1 cut(s) 81
AspS9I GGNCC 1 cut(s) 303
AsuHPI GGTGA 1 cut(s) 287
BaeGI GKGCMC 1 cut(s) 85
Bbv12I GWGCWC 1 cut(s) 85
BfmI CTRYAG 1 cut(s) 282
BglII AGATCT 1 cut(s) 135
BmgT120I GGNCC 1 cut(s) 303
BmsI GCATC 1 cut(s) 250
BsaBI GATNNNNATC 1 cut(s) 225
BsaJI CCNNGG 1 cut(s) 255
Bse3DI GCAATG 1 cut(s) 64
Bse8I GATNNNNATC 1 cut(s) 225
BseDI CCNNGG 1 cut(s) 255
BseGI GGATG 1 cut(s) 265
BseJI GATNNNNATC 1 cut(s) 225
BseMI GCAATG 1 cut(s) 64
BseMII CTCAG 1 cut(s) 32
BseSI GKGCMC 1 cut(s) 85
BshFI GGCC 1 cut(s) 304
BsiHKAI GWGCWC 1 cut(s) 85
BsnI GGCC 1 cut(s) 304
Bsp1286I GDGCHC 1 cut(s) 85
Bsp143I GATC 2 cut(s) 61, 135
Bsp19I CCATGG 1 cut(s) 255
BspANI GGCC 1 cut(s) 304
BspCNI CTCAG 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 64
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 2 cut(s) 61, 135
BssT1I CCWWGG 1 cut(s) 255
Bst6I CTCTTC 1 cut(s) 145
BstDEI CTNAG 2 cut(s) 18, 330
BstDSI CCRYGG 1 cut(s) 255
BstF5I GGATG 1 cut(s) 265
BstKTI GATC 2 cut(s) 64, 138
BstMBI GATC 2 cut(s) 61, 135
BstSFI CTRYAG 1 cut(s) 282
BstSLI GKGCMC 1 cut(s) 85
BstX2I RGATCY 1 cut(s) 135
BstYI RGATCY 1 cut(s) 135
BsuRI GGCC 1 cut(s) 304
BtgI CCRYGG 1 cut(s) 255
BtsCI GGATG 1 cut(s) 265
Cfr13I GGNCC 1 cut(s) 303
Csp6I GTAC 1 cut(s) 156
CviAII CATG 2 cut(s) 256, 300
CviJI RGCY 4 cut(s) 125, 281, 304, 312
CviKI_1 RGCY 4 cut(s) 125, 281, 304, 312
CviQI GTAC 1 cut(s) 156
DdeI CTNAG 2 cut(s) 18, 330
DpnI GATC 2 cut(s) 63, 137
DpnII GATC 2 cut(s) 61, 135
Eam1104I CTCTTC 1 cut(s) 145
EarI CTCTTC 1 cut(s) 145
Eco130I CCWWGG 1 cut(s) 255
Eco57I CTGAAG 1 cut(s) 147
EcoT14I CCWWGG 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 2 cut(s) 259, 303
FaiI YATR 5 cut(s) 105, 236, 246, 257, 301
FatI CATG 2 cut(s) 255, 299
FblI GTMKAC 1 cut(s) 37
FokI GGATG 1 cut(s) 272
HaeIII GGCC 1 cut(s) 304
Hin1II CATG 2 cut(s) 259, 303
HincII GTYRAC 1 cut(s) 38
HindII GTYRAC 1 cut(s) 38
HphI GGTGA 1 cut(s) 287
Hpy166II GTNNAC 2 cut(s) 38, 83
Hpy188I TCNGA 1 cut(s) 231
Hpy188III TCNNGA 1 cut(s) 65
Hpy8I GTNNAC 2 cut(s) 38, 83
HpyAV CCTTC 2 cut(s) 122, 190
HpyCH4V TGCA 2 cut(s) 83, 299
HpyF3I CTNAG 2 cut(s) 18, 330
Hsp92II CATG 2 cut(s) 259, 303
Kzo9I GATC 2 cut(s) 61, 135
LmnI GCTCC 3 cut(s) 55, 317, 330
LweI GCATC 1 cut(s) 250
MaeIII GTNAC 1 cut(s) 21
MalI GATC 2 cut(s) 63, 137
MboI GATC 2 cut(s) 61, 135
MboII GAAGA 5 cut(s) 87, 119, 122, 132, 145
MflI RGATCY 1 cut(s) 135
MhlI GDGCHC 1 cut(s) 85
MluCI AATT 2 cut(s) 91, 100
MmeI TCCRAC 1 cut(s) 173
MnlI CCTC 1 cut(s) 186
MseI TTAA 1 cut(s) 318
MslI CAYNNNNRTG 1 cut(s) 320
NcoI CCATGG 1 cut(s) 255
NdeII GATC 2 cut(s) 61, 135
NlaIII CATG 2 cut(s) 259, 303
NmuCI GTSAC 1 cut(s) 21
PspPI GGNCC 1 cut(s) 303
PsuI RGATCY 1 cut(s) 135
RsaI GTAC 1 cut(s) 157
RsaNI GTAC 1 cut(s) 156
RseI CAYNNNNRTG 1 cut(s) 320
SalI GTCGAC 1 cut(s) 36
SaqAI TTAA 1 cut(s) 318
Sau3AI GATC 2 cut(s) 61, 135
Sau96I GGNCC 1 cut(s) 303
SduI GDGCHC 1 cut(s) 85
SetI ASST 3 cut(s) 201, 283, 314
SfaNI GCATC 1 cut(s) 250
SfcI CTRYAG 1 cut(s) 282
SmiMI CAYNNNNRTG 1 cut(s) 320
Sse9I AATT 2 cut(s) 91, 100
StyI CCWWGG 1 cut(s) 255
TaqI TCGA 1 cut(s) 37
TasI AATT 2 cut(s) 91, 100
TatI WGTACW 1 cut(s) 155
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TseFI GTSAC 1 cut(s) 21
Tsp45I GTSAC 1 cut(s) 21
TspDTI ATGAA 3 cut(s) 120, 129, 155
VneI GTGCAC 1 cut(s) 81
XmiI GTMKAC 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.