FvH4_6g53590

50S ribosomal protein L27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009984
Physical Location & Seq
Forward (+)
39266812 .. 39268472
1661 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g53590.t1

Sequence Viewer

Length: 603 bp
ATGGCGGTCGCGTCTATGAGTTTCAACCTAGTCGGAGCATTCAGAGGGATGGCTCTGAGCTCAAGCTTCTCAACCTCCTCTTCTTCGTCCTCCTCTTTCTTCAGAGGTGACTTGGGTTCCCTACAGTTGGGTCCAAAGCTGTCATTGTCTACAACCCAACGGAGATTGCCGTTGACGATTCAAAACGCTCACAAGAAAGGAGCTGGGAGTACCAAGAACGGTCGCGATTCCAAAGGCCAAAGGCTCGGCGTTAAGATTTACGGCGACCAGGTGGCCAAGCCCGGCGCTATCATCATACGTCAGCGTGGCACCAAGTTCCATGCGGGGAAGAATGTAGGGCTCGGCAAGGACCATACCATCTTCTCCTTGATAGATGGCTTGGTTAAGTTTGAGAAGTTCGGACCAGACAGGAAGAAGGTGAGTGTTTACCCTCGGGAGATACAGCCTGAAAACCCCAACAGCTACAGGGTTAGGAAGAGGGAGAACTTCAAGCTACAACGCGAGAAAAAGAAAGCAAGAAAGGAAGCCTATATCCTTCAGAACAAACCACAACTGGTTCTTGCATCTGCAGATAATGGTGCAGAGAACAATGCTCTTTGTTGA

Protein Analysis

201

Amino Acids

21.93

Weight (kDa)

10.55

Isoelectric Point (pI)

24.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27 PF01016 63 - 142 2.2e-35 Ribosomal L27 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 308
AccI GTMKAC 1 cut(s) 149
AccII CGCG 3 cut(s) 11, 225, 501
AciI CCGC 2 cut(s) 5, 323
AcoI YGGCCR 1 cut(s) 273
AcuI CTGAAG 2 cut(s) 85, 521
AfaI GTAC 1 cut(s) 211
AfiI CCNNNNNNNGG 1 cut(s) 127
AgsI TTSAA 3 cut(s) 25, 182, 490
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 6 cut(s) 60, 66, 139, 203, 462, 493
AluI AGCT 6 cut(s) 60, 66, 139, 203, 462, 493
Alw21I GWGCWC 1 cut(s) 62
Ama87I CYCGRG 1 cut(s) 432
AoxI GGCC 2 cut(s) 235, 273
AspLEI GCGC 1 cut(s) 287
AspS9I GGNCC 3 cut(s) 131, 349, 401
AsuC2I CCSGG 1 cut(s) 282
AsuHPI GGTGA 2 cut(s) 119, 430
AvaI CYCGRG 1 cut(s) 432
AvaII GGWCC 3 cut(s) 131, 349, 401
BalI TGGCCA 1 cut(s) 275
BanI GGYRCC 1 cut(s) 308
BanII GRGCYC 2 cut(s) 62, 342
Bbv12I GWGCWC 1 cut(s) 62
BccI CCATC 3 cut(s) 43, 365, 368
BceAI ACGGC 2 cut(s) 154, 277
BciT130I CCWGG 1 cut(s) 269
BcnI CCSGG 1 cut(s) 282
BfaI CTAG 1 cut(s) 29
BfmI CTRYAG 3 cut(s) 122, 463, 567
BfoI RGCGCY 1 cut(s) 288
Bme1390I CCNGG 2 cut(s) 269, 282
Bme18I GGWCC 3 cut(s) 131, 349, 401
BmeT110I CYCGRG 1 cut(s) 432
BmgT120I GGNCC 3 cut(s) 131, 349, 401
BmiI GGNNCC 3 cut(s) 118, 132, 310
BmrFI CCNGG 2 cut(s) 269, 282
BmsI GCATC 1 cut(s) 572
BpuEI CTTGAG 1 cut(s) 46
BpuMI CCSGG 1 cut(s) 282
BsaJI CCNNGG 1 cut(s) 431
Bsc4I CCNNNNNNNGG 1 cut(s) 127
Bse1I ACTGG 1 cut(s) 558
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 1 cut(s) 431
BseGI GGATG 1 cut(s) 54
BseLI CCNNNNNNNGG 1 cut(s) 127
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 558
BseRI GAGGAG 2 cut(s) 67, 82
BseYI CCCAGC 1 cut(s) 203
BsgI GTGCAG 1 cut(s) 600
Bsh1236I CGCG 3 cut(s) 11, 225, 501
Bsh1285I CGRYCG 2 cut(s) 9, 223
BshFI GGCC 2 cut(s) 237, 275
BshNI GGYRCC 1 cut(s) 308
BsiEI CGRYCG 2 cut(s) 9, 223
BsiHKAI GWGCWC 1 cut(s) 62
BsiHKCI CYCGRG 1 cut(s) 432
BsiSI CCGG 1 cut(s) 282
BslI CCNNNNNNNGG 1 cut(s) 127
BsmI GAATGC 1 cut(s) 38
BsnI GGCC 2 cut(s) 237, 275
BsoBI CYCGRG 1 cut(s) 432
Bsp1286I GDGCHC 2 cut(s) 62, 342
Bsp68I TCGCGA 1 cut(s) 225
BspACI CCGC 2 cut(s) 5, 323
BspANI GGCC 2 cut(s) 237, 275
BspCNI CTCAG 1 cut(s) 48
BspFNI CGCG 3 cut(s) 11, 225, 501
BspLI GGNNCC 3 cut(s) 118, 132, 310
BspMAI CTGCAG 1 cut(s) 571
BspT107I GGYRCC 1 cut(s) 308
BsrI ACTGG 1 cut(s) 558
BssECI CCNNGG 1 cut(s) 431
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 2 cut(s) 126, 221
Bst6I CTCTTC 2 cut(s) 85, 470
BstDEI CTNAG 1 cut(s) 56
BstF5I GGATG 1 cut(s) 54
BstFNI CGCG 3 cut(s) 11, 225, 501
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 1 cut(s) 287
BstMCI CGRYCG 2 cut(s) 9, 223
BstNI CCWGG 1 cut(s) 269
BstSCI CCNGG 2 cut(s) 267, 280
BstSFI CTRYAG 3 cut(s) 122, 463, 567
BstUI CGCG 3 cut(s) 11, 225, 501
BsuRI GGCC 2 cut(s) 237, 275
BtsCI GGATG 1 cut(s) 54
BtuMI TCGCGA 1 cut(s) 225
CfoI GCGC 1 cut(s) 287
Cfr13I GGNCC 3 cut(s) 131, 349, 401
CsiI ACCWGGT 1 cut(s) 267
Csp6I GTAC 1 cut(s) 210
CviAII CATG 1 cut(s) 320
CviQI GTAC 1 cut(s) 210
DdeI CTNAG 1 cut(s) 56
EaeI YGGCCR 1 cut(s) 273
Eam1104I CTCTTC 2 cut(s) 85, 470
EarI CTCTTC 2 cut(s) 85, 470
Ecl136II GAGCTC 1 cut(s) 60
Eco24I GRGCYC 2 cut(s) 62, 342
Eco47I GGWCC 3 cut(s) 131, 349, 401
Eco53kI GAGCTC 1 cut(s) 60
Eco57I CTGAAG 2 cut(s) 85, 521
Eco88I CYCGRG 1 cut(s) 432
EcoICRI GAGCTC 1 cut(s) 60
EcoRII CCWGG 1 cut(s) 267
EcoT38I GRGCYC 2 cut(s) 62, 342
FaeI CATG 1 cut(s) 323
FaiI YATR 5 cut(s) 17, 296, 321, 354, 531
FatI CATG 1 cut(s) 319
FauI CCCGC 1 cut(s) 316
FblI GTMKAC 1 cut(s) 149
FokI GGATG 1 cut(s) 61
FriOI GRGCYC 2 cut(s) 62, 342
FspBI CTAG 1 cut(s) 29
GlaI GCGC 1 cut(s) 286
GsaI CCCAGC 1 cut(s) 207
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 2 cut(s) 237, 275
HapII CCGG 1 cut(s) 282
HhaI GCGC 1 cut(s) 287
Hin1II CATG 1 cut(s) 323
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HincII GTYRAC 1 cut(s) 174
HindII GTYRAC 1 cut(s) 174
HindIII AAGCTT 1 cut(s) 64
HinfI GANTC 2 cut(s) 178, 227
HpaII CCGG 1 cut(s) 282
HphI GGTGA 2 cut(s) 119, 430
Hpy166II GTNNAC 3 cut(s) 150, 174, 427
Hpy188I TCNGA 6 cut(s) 35, 44, 57, 104, 401, 540
Hpy188III TCNNGA 2 cut(s) 224, 434
Hpy8I GTNNAC 3 cut(s) 150, 174, 427
HpyAV CCTTC 2 cut(s) 409, 545
HpyCH4III ACNGT 2 cut(s) 126, 221
HpyCH4IV ACGT 1 cut(s) 298
HpyCH4V TGCA 3 cut(s) 563, 569, 581
HpyF3I CTNAG 1 cut(s) 56
HpySE526I ACGT 1 cut(s) 298
Hsp92II CATG 1 cut(s) 323
HspAI GCGC 1 cut(s) 285
LmnI GCTCC 2 cut(s) 35, 200
LpnPI CCDG 9 cut(s) 189, 254, 281, 295, 394, 417, 451, 459, 539
LweI GCATC 1 cut(s) 572
MabI ACCWGGT 1 cut(s) 267
MaeI CTAG 1 cut(s) 29
MaeII ACGT 1 cut(s) 298
MaeIII GTNAC 1 cut(s) 107
MboII GAAGA 7 cut(s) 72, 75, 91, 340, 352, 424, 487
MhlI GDGCHC 2 cut(s) 62, 342
MlsI TGGCCA 1 cut(s) 275
MluNI TGGCCA 1 cut(s) 275
MmeI TCCRAC 1 cut(s) 13
MnlI CCTC 8 cut(s) 38, 85, 88, 98, 100, 103, 441, 471
Mox20I TGGCCA 1 cut(s) 275
MscI TGGCCA 1 cut(s) 275
MseI TTAA 2 cut(s) 252, 384
Msp20I TGGCCA 1 cut(s) 275
MspI CCGG 1 cut(s) 282
MspR9I CCNGG 2 cut(s) 269, 282
Mva1269I GAATGC 1 cut(s) 38
MvaI CCWGG 1 cut(s) 269
MvnI CGCG 3 cut(s) 11, 225, 501
NciI CCSGG 1 cut(s) 282
NlaIII CATG 1 cut(s) 323
NlaIV GGNNCC 3 cut(s) 118, 132, 310
NmeAIII GCCGAG 2 cut(s) 225, 321
NmuCI GTSAC 1 cut(s) 107
NruI TCGCGA 1 cut(s) 225
PctI GAATGC 1 cut(s) 38
PfeI GAWTC 2 cut(s) 178, 227
Psp124BI GAGCTC 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 267
PspFI CCCAGC 1 cut(s) 203
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 3 cut(s) 118, 132, 310
PspPI GGNCC 3 cut(s) 131, 349, 401
PstI CTGCAG 1 cut(s) 571
RruI TCGCGA 1 cut(s) 225
RsaI GTAC 1 cut(s) 211
RsaNI GTAC 1 cut(s) 210
SacI GAGCTC 1 cut(s) 62
SaqAI TTAA 2 cut(s) 252, 384
Sau96I GGNCC 3 cut(s) 131, 349, 401
ScrFI CCNGG 2 cut(s) 269, 282
SduI GDGCHC 2 cut(s) 62, 342
SexAI ACCWGGT 1 cut(s) 267
SfaNI GCATC 1 cut(s) 572
SfcI CTRYAG 3 cut(s) 122, 463, 567
SinI GGWCC 3 cut(s) 131, 349, 401
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
SsiI CCGC 2 cut(s) 5, 323
SspMI CTAG 1 cut(s) 29
SstI GAGCTC 1 cut(s) 62
StyD4I CCNGG 2 cut(s) 267, 280
TaaI ACNGT 2 cut(s) 126, 221
TaiI ACGT 1 cut(s) 301
TfiI GAWTC 2 cut(s) 178, 227
Tru1I TTAA 2 cut(s) 252, 384
Tru9I TTAA 2 cut(s) 252, 384
TseFI GTSAC 1 cut(s) 107
Tsp45I GTSAC 1 cut(s) 107
TspGWI ACGGA 1 cut(s) 175
VpaK11BI GGWCC 3 cut(s) 131, 349, 401
XmiI GTMKAC 1 cut(s) 149
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.