Rw2G054600

50S ribosomal protein L27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009984
Physical Location & Seq
Forward (+)
82175320 .. 82176452
1133 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G054600.1

Sequence Viewer

Length: 603 bp
ATGGCGGTCGCGTCTATGAGTTTCAACCTAGTCGGAGCATTCAGAGGGATGGCTCTGAGCTCAAGCTCCTCAAGCTCTTCCTCTTCCTCCTCTTTCTTCAGAGGCGATTTGGGCTCCCTACAATTGGGCCCAAAGCTATCCTTGTCTACTACTACCCAACGCAGATTGCCATTGACGATTCAAAACGCGCACAAGAAAGGAGCTGGTAGTACCAAGAACGGTCGCGATTCCAAAGGCCAAAGGCTCGGCGTCAAGATTTACGGTGACCAGGTCGCCAAGCCCGGCTCCATCATCATACGTCAGCGGGGCACCAAGTTCCATGCGGGGAAGAATGTGGGGCTTGGCAAGGACCATACCATTTTCTCTCTGATTGATGGGTTGGTTAAGTTTGAGAAGTATGGACCTGACAGGAAGAAGGTGAGTGTTTACCCTCGGGAGATACAGCCTGAAAATCCCAACAGCTACAGGGTTAGGAAGAGGGAGAACTTCAAGCTACAACGCGAGAAAAAGAAAGCAAGAAAGGAAGGCTATATTCTTCAAAACAAACCACAACTGGTGCTTGCATCTGCTGATAATGGTGCAGAGACCAATCCTCTTTGTTGA

Protein Analysis

200

Amino Acids

21.9

Weight (kDa)

10.48

Isoelectric Point (pI)

26.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27 PF01016 63 - 142 8.9e-36 Ribosomal L27 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 308
AccI GTMKAC 1 cut(s) 146
AccII CGCG 4 cut(s) 11, 188, 225, 501
AciI CCGC 3 cut(s) 5, 304, 323
AcuI CTGAAG 1 cut(s) 82
AcyI GRCGYC 1 cut(s) 249
AfaI GTAC 1 cut(s) 211
AfiI CCNNNNNNNGG 1 cut(s) 124
AgsI TTSAA 4 cut(s) 25, 182, 490, 539
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 7 cut(s) 60, 66, 75, 136, 203, 462, 493
AluI AGCT 7 cut(s) 60, 66, 75, 136, 203, 462, 493
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 578
Ama87I CYCGRG 1 cut(s) 432
AoxI GGCC 2 cut(s) 127, 235
ApaI GGGCCC 1 cut(s) 131
AspLEI GCGC 1 cut(s) 190
AspS9I GGNCC 4 cut(s) 127, 128, 349, 401
AsuC2I CCSGG 1 cut(s) 282
AsuHPI GGTGA 2 cut(s) 275, 430
AvaI CYCGRG 1 cut(s) 432
AvaII GGWCC 2 cut(s) 349, 401
BaeGI GKGCMC 2 cut(s) 131, 311
BanI GGYRCC 1 cut(s) 308
BanII GRGCYC 3 cut(s) 62, 116, 131
Bbv12I GWGCWC 1 cut(s) 62
BccI CCATC 3 cut(s) 43, 296, 368
BciT130I CCWGG 1 cut(s) 269
BcnI CCSGG 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 578
BfaI CTAG 1 cut(s) 29
BfmI CTRYAG 1 cut(s) 463
Bme1390I CCNGG 2 cut(s) 269, 282
Bme18I GGWCC 2 cut(s) 349, 401
BmeT110I CYCGRG 1 cut(s) 432
BmgT120I GGNCC 4 cut(s) 127, 128, 349, 401
BmiI GGNNCC 4 cut(s) 115, 129, 286, 310
BmrFI CCNGG 2 cut(s) 269, 282
BmsI GCATC 1 cut(s) 572
BpuEI CTTGAG 2 cut(s) 46, 55
BpuMI CCSGG 1 cut(s) 282
BsaHI GRCGYC 1 cut(s) 249
BsaI GGTCTC 1 cut(s) 578
BsaJI CCNNGG 1 cut(s) 431
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse1I ACTGG 1 cut(s) 558
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 1 cut(s) 431
BseGI GGATG 1 cut(s) 54
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 558
BseRI GAGGAG 2 cut(s) 58, 79
BseSI GKGCMC 2 cut(s) 131, 311
BsgI GTGCAG 1 cut(s) 600
Bsh1236I CGCG 4 cut(s) 11, 188, 225, 501
Bsh1285I CGRYCG 2 cut(s) 9, 223
BshFI GGCC 2 cut(s) 129, 237
BshNI GGYRCC 1 cut(s) 308
BsiEI CGRYCG 2 cut(s) 9, 223
BsiHKAI GWGCWC 1 cut(s) 62
BsiHKCI CYCGRG 1 cut(s) 432
BsiSI CCGG 1 cut(s) 282
BslI CCNNNNNNNGG 1 cut(s) 124
BsmAI GTCTC 1 cut(s) 578
BsmI GAATGC 1 cut(s) 38
BsnI GGCC 2 cut(s) 129, 237
Bso31I GGTCTC 1 cut(s) 578
BsoBI CYCGRG 1 cut(s) 432
Bsp120I GGGCCC 1 cut(s) 127
Bsp1286I GDGCHC 4 cut(s) 62, 116, 131, 311
Bsp68I TCGCGA 1 cut(s) 225
BspACI CCGC 3 cut(s) 5, 304, 323
BspANI GGCC 2 cut(s) 129, 237
BspCNI CTCAG 1 cut(s) 48
BspFNI CGCG 4 cut(s) 11, 188, 225, 501
BspLI GGNNCC 4 cut(s) 115, 129, 286, 310
BspQI GCTCTTC 1 cut(s) 82
BspT107I GGYRCC 1 cut(s) 308
BspTNI GGTCTC 1 cut(s) 578
BsrI ACTGG 1 cut(s) 558
BssECI CCNNGG 1 cut(s) 431
BssNI GRCGYC 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 2 cut(s) 221, 263
Bst6I CTCTTC 3 cut(s) 82, 88, 470
BstACI GRCGYC 1 cut(s) 249
BstC8I GCNNGC 1 cut(s) 561
BstDEI CTNAG 1 cut(s) 56
BstEII GGTNACC 1 cut(s) 263
BstF5I GGATG 1 cut(s) 54
BstFNI CGCG 4 cut(s) 11, 188, 225, 501
BstHHI GCGC 1 cut(s) 190
BstMAI GTCTC 1 cut(s) 578
BstMCI CGRYCG 2 cut(s) 9, 223
BstMWI GCNNNNNNNGC 2 cut(s) 72, 111
BstNI CCWGG 1 cut(s) 269
BstPI GGTNACC 1 cut(s) 263
BstSCI CCNGG 2 cut(s) 267, 280
BstSFI CTRYAG 1 cut(s) 463
BstSLI GKGCMC 2 cut(s) 131, 311
BstUI CGCG 4 cut(s) 11, 188, 225, 501
BsuRI GGCC 2 cut(s) 129, 237
BtsCI GGATG 1 cut(s) 54
BtuMI TCGCGA 1 cut(s) 225
Cac8I GCNNGC 1 cut(s) 561
CfoI GCGC 1 cut(s) 190
Cfr13I GGNCC 4 cut(s) 127, 128, 349, 401
CseI GACGC 1 cut(s) 238
CsiI ACCWGGT 1 cut(s) 267
Csp6I GTAC 1 cut(s) 210
CviAII CATG 1 cut(s) 320
CviQI GTAC 1 cut(s) 210
DdeI CTNAG 1 cut(s) 56
Eam1104I CTCTTC 3 cut(s) 82, 88, 470
EarI CTCTTC 3 cut(s) 82, 88, 470
Ecl136II GAGCTC 1 cut(s) 60
Eco24I GRGCYC 3 cut(s) 62, 116, 131
Eco31I GGTCTC 1 cut(s) 578
Eco47I GGWCC 2 cut(s) 349, 401
Eco53kI GAGCTC 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 82
Eco88I CYCGRG 1 cut(s) 432
Eco91I GGTNACC 1 cut(s) 263
EcoICRI GAGCTC 1 cut(s) 60
EcoO65I GGTNACC 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 267
EcoT38I GRGCYC 3 cut(s) 62, 116, 131
FaeI CATG 1 cut(s) 323
FaiI YATR 6 cut(s) 17, 296, 321, 354, 399, 531
FalI AAGNNNNNCTT 2 cut(s) 125, 157
FatI CATG 1 cut(s) 319
FauI CCCGC 2 cut(s) 297, 316
FblI GTMKAC 1 cut(s) 146
FokI GGATG 1 cut(s) 61
FriOI GRGCYC 3 cut(s) 62, 116, 131
FspBI CTAG 1 cut(s) 29
GlaI GCGC 1 cut(s) 189
HaeIII GGCC 2 cut(s) 129, 237
HapII CCGG 1 cut(s) 282
HgaI GACGC 1 cut(s) 238
HhaI GCGC 1 cut(s) 190
Hin1I GRCGYC 1 cut(s) 249
Hin1II CATG 1 cut(s) 323
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HinfI GANTC 2 cut(s) 178, 227
HpaII CCGG 1 cut(s) 282
HphI GGTGA 2 cut(s) 275, 430
Hpy166II GTNNAC 2 cut(s) 147, 427
Hpy188I TCNGA 5 cut(s) 35, 44, 57, 101, 369
Hpy188III TCNNGA 3 cut(s) 224, 253, 434
Hpy8I GTNNAC 2 cut(s) 147, 427
HpyAV CCTTC 2 cut(s) 409, 518
HpyCH4III ACNGT 2 cut(s) 221, 263
HpyCH4IV ACGT 1 cut(s) 298
HpyCH4V TGCA 2 cut(s) 563, 581
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 111
HpyF3I CTNAG 1 cut(s) 56
HpySE526I ACGT 1 cut(s) 298
Hsp92I GRCGYC 1 cut(s) 249
Hsp92II CATG 1 cut(s) 323
HspAI GCGC 1 cut(s) 188
LguI GCTCTTC 1 cut(s) 82
LmnI GCTCC 5 cut(s) 35, 71, 119, 200, 290
LpnPI CCDG 9 cut(s) 189, 254, 281, 295, 394, 417, 451, 459, 539
LweI GCATC 1 cut(s) 572
MabI ACCWGGT 1 cut(s) 267
MaeI CTAG 1 cut(s) 29
MaeII ACGT 1 cut(s) 298
MaeIII GTNAC 1 cut(s) 263
MboII GAAGA 7 cut(s) 69, 75, 88, 340, 424, 487, 527
MfeI CAATTG 1 cut(s) 122
MhlI GDGCHC 4 cut(s) 62, 116, 131, 311
MluCI AATT 1 cut(s) 122
MmeI TCCRAC 1 cut(s) 13
MnlI CCTC 9 cut(s) 38, 79, 91, 95, 97, 100, 441, 471, 603
MseI TTAA 1 cut(s) 384
MspA1I CMGCKG 1 cut(s) 304
MspI CCGG 1 cut(s) 282
MspR9I CCNGG 2 cut(s) 269, 282
MunI CAATTG 1 cut(s) 122
Mva1269I GAATGC 1 cut(s) 38
MvaI CCWGG 1 cut(s) 269
MvnI CGCG 4 cut(s) 11, 188, 225, 501
MwoI GCNNNNNNNGC 2 cut(s) 72, 111
NciI CCSGG 1 cut(s) 282
NlaIII CATG 1 cut(s) 323
NlaIV GGNNCC 4 cut(s) 115, 129, 286, 310
NmeAIII GCCGAG 1 cut(s) 225
NmuCI GTSAC 1 cut(s) 263
NruI TCGCGA 1 cut(s) 225
PciSI GCTCTTC 1 cut(s) 82
PctI GAATGC 1 cut(s) 38
PfeI GAWTC 2 cut(s) 178, 227
PflFI GACNNNGTC 1 cut(s) 269
Psp124BI GAGCTC 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 267
PspEI GGTNACC 1 cut(s) 263
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 4 cut(s) 115, 129, 286, 310
PspOMI GGGCCC 1 cut(s) 127
PspPI GGNCC 4 cut(s) 127, 128, 349, 401
PsyI GACNNNGTC 1 cut(s) 269
RruI TCGCGA 1 cut(s) 225
RsaI GTAC 1 cut(s) 211
RsaNI GTAC 1 cut(s) 210
SacI GAGCTC 1 cut(s) 62
SapI GCTCTTC 1 cut(s) 82
SaqAI TTAA 1 cut(s) 384
Sau96I GGNCC 4 cut(s) 127, 128, 349, 401
ScrFI CCNGG 2 cut(s) 269, 282
SduI GDGCHC 4 cut(s) 62, 116, 131, 311
SexAI ACCWGGT 1 cut(s) 267
SfaNI GCATC 1 cut(s) 572
SfcI CTRYAG 1 cut(s) 463
SinI GGWCC 2 cut(s) 349, 401
SmlI CTYRAG 2 cut(s) 61, 70
SmoI CTYRAG 2 cut(s) 61, 70
Sse9I AATT 1 cut(s) 122
SsiI CCGC 3 cut(s) 5, 304, 323
SspMI CTAG 1 cut(s) 29
SstI GAGCTC 1 cut(s) 62
StyD4I CCNGG 2 cut(s) 267, 280
TaaI ACNGT 2 cut(s) 221, 263
TaiI ACGT 1 cut(s) 301
TasI AATT 1 cut(s) 122
TfiI GAWTC 2 cut(s) 178, 227
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TseFI GTSAC 1 cut(s) 263
Tsp45I GTSAC 1 cut(s) 263
Tth111I GACNNNGTC 1 cut(s) 269
VpaK11BI GGWCC 2 cut(s) 349, 401
XmiI GTMKAC 1 cut(s) 146
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.