Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003778

Physical Location & Seq

Genomic Coordinates Forward (+)

16945165 .. 16953353

Gene Structure 8189 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G42390.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3798 bp

ATGGCTTCATCGTCCTCTTCCATTTTCACCGGTGTTAAGTTCTCTCCGATCTTAGCTCCCTTTAACTCCGGAGATAGCCGCCGCTCTCGATATCTAAAAGATAGCCGGAATAAAGTTAGGTTTAATCCATCGTCGCCGCGTCTCACTCCTCATCGTGTTCGCGTCGAAGCTCCGTCTTTAATTCCCTATAATGGTCTTTGGGCGGCGCAGCCAAATTCGCATAAGGGACGTTTAAAGAGGAACATTGTTTCGGGAAAAGAAGCTACCGGGATATCACTTTCTCAAGGACGTAATTTCTGTCTTACTTGTAAAAGGAATCAAGCTGGAATCAGAAGAGCATTACCTAGTGCCTTTGTGGATAGAACTGCATTTAGTTTGTCGAGGTCGAGTTTGACATCTTCTCTGAGGAAACATTCTCAGATTGTGAATGCAACTCTTGGACCAGATGAGCCACATGCTGCTGGTACAGCTTGGCCTGATGGTATTGTTGCGGAGAGACAAGATCTCGACTTATTGCCTCCTGAGATTGATAGTGCAGAGCTAGAAGCGTTTCTTGGTTGTGAACTTCCTTCTCATCCAAAGTTGCACCGGGGTCAATTGAAAAATGGGCTTCGATATCTTATTTTGCCAAACAAAGTTCCACCGAACAGATTTGAGGCACACATGGAAGTTCATGTAGGATCGATTGATGAGGAAGAAGATGAGCAAGGGATTGCTCATATGATAGAACATGTTGCTTTCCTTGGGAGCAAGAAACGTGAGAAACTTCTTGGTACAGGTGCCCGCTCTAATGCCTACACCGATTTCCACCATACAGTATTTCATATTCATTCTCCAACCCACACGAAGGACTCTGAGGATGACCTCTTCCCATCTGTGCTCGATGCCTTAAATGAGATAGCCTTTCACCCAAAATTTCTCTCTTCTCGAGTTGAGAAAGAAAGGCGGGCCATACTTTCAGAACTTCAGATGATGAATACAATTGAGTATCGTGTTGACTGCCAGTTGTTGCAACATCTACATTCTGAGAACAAGCTGGGTAGGAGGTTCCCTATTGGATTGGAAGAGCAGATTAAGAAGTGGGATGTTGACAAAATTAGAAAATTTCATGAGCGATGGTACTTCCCAGCCAATGCCACTCTATATATTGTGGGAGATATTGACAACATACCTCGGATAGTCCACAATATTGAAGCTGTGTTTGGAAAAAATGGTTTGGATAATGAATCAACACCCAGTTCTCCTAGTCCTGGTGCCTTCGGTGCCATGGCTAATTTTCTCGTTCCAAAGCTGCCAGCTGGTCTTGGTGGTACCTTTTCAAATGAGAAAACAAATACTGCTGATCAGTCTAAGATGATTAAAAGAGAAAGACATGCAATTCGTCCCCCGGTTGAGCATAATTGGTCGCTTCCTGGAACAAGTGTTGATCTAAAGCCTCCACAGATATTTAAGCACGAGCTTCTTCAGAATTTTGCAATCAACATGTTTTGCAAGATACCTGTTAGCAAGGTTCAGACATTTGGAGACCTGCGAAATGTTCTGATGAAAAGAATATTTCTCTCTGCTCTACATTTTCGAATTAATACGAGATACAAGAGTTCAAACCCACCATTTACGTCTGTTGAACTGGACCATAGTGATTCGGGAAGGGAAGGATGCACTGTTACGACTCTTACTGTCACCGCAGAGCCCCAAAATTGGCAAAATGCAGTCAAAGTTGCTGTCCAAGAGGTTCGAAGACTTAAAGAATTTGGTGTCACCAGGGGTGAACTTACACGTTACATGGATGCTCTTCTAAAAGATAGTGAACATCTTGCAGCTATGATAGATAATGTGTCTTCTGTTGATAACCTGGATTTTATCATGGAAAGTGATGCACTAAGCCACACGGTTATGGATCAGACGCAAGGACATGAGACCTTGGTTGCTGTAGCTGGAACTGTTACTCTTGAAGAGGTAAACACTGTTGGCGCCAAGGTGCTAGAATTTATTTCTGATTTCGGAAGACCCACTGCCCCTCTTCCCGCAGCAATAGTTGCTTGTGTTCCCACTAAGGTGCATGTTGATGGGGTTGGTGAGAGTGATTTCAATATATCTCCTGATGAAATAATAGAGTCTGTCAAATCAGGATTATTAGCACCCATTGAAGCTGAGCCTGAGCTCGAGGTGCCAAAAGAATTGATCTCTCAATCACAATTGAAAGAGTTAACCTTGCAGAGAAATCCATGTTTTGTCCCTATTCCTGGGTCAGGTTTAACCAAATTGCATGACAAAGAGACTGGGATCACTCAGCTTCGGTTATCCAATGGGATAGCTGTAAATTACAAGAAATCTACGACCGAATCCCGGGCAGGTGTCATGCGGCTCATTGTTGGTGGTGGACGAGCTGCTGAAACATCTGATTCCAAAGGAGCTGTGGTTGTTGGTGTTCGTACACTCAGCGAGGGTGGTCGAGTTGGAGACTTTTCAAGGGAGCAGGTGGAGCTGTTCTGTGTAAATCATTTGATAAACTGTTCGTTGGAGTCAACTGAGGAGTTCATTGCCATGGAATTCCGGTTTACTCTGAGAGACAATGGAATGCAAGCGGCTTTTCAGCTGCTTCACATGGTGCTTGAGCGTAGTGTCTGGTTGGAAGATGCATTTGATCGAGCAAGGCAGCTATATCTTTCGTATTTTCGATCAATTCCTAAAAGCTTGGAGCGTGCGACTGCTCATAAACTTATGATCGCTATGCTGAACGGTGATGAGCGTTTTGTTGAGCCGACACCTAAGTCACTACAGAGCTTGAATCTAGAATCTGTGAAAGATGCGGTCATGAGTCATTTTGTTGGGGACAATATGGAGGTAAGCATTGTGGGGGACTTTTCAGAGGAGGAAATCGAGCGTTGCATCCTTGATTACCTTGGTACAGTTAAAGCTAGCCATGATTCTGCAAAACCACCAGGATCTGAGCCTATCTTGTTTCGCCAACCTACAGCTGGCTTGCAATTTCAACAGGTATTCTTGAAGGATACTGATGAGAGAGCATGTGCATACATTGCGGGACCAGCGCCAAATCGATGGGGCTTTACAGTTGACGGGGATGACCTTTTTCAGTCTGTTAGCAAACTTCCTGTTGCTCATGATGGACTTTTGAAATCGGAGGAACAATTGCTAGAGGGAGGAGACAGGGAGTTGCAAAAGAAGCTTCGTGCCCACCCTCTTTTCTTTGGAGTTACAATGGGTCTTCTGGCCGAAATCATAAACTCAAGGCTTTTCACGACAGTAAGAGATTCTCTTGGACTGACATATGACGTATCATTTGAGCTAAACCTATTTGATAGGCTGAATCTCGGGTGGTATGTGATATCTGTGACTTCAACTCCCGGCAAGGTATACAAAGCGGTTGATGCCTGTAAGAGTGTTCTTAGAGGTTTGCATAGCAACCAAATTGCCCCTAGGGAGTTGGATAGGGCAAAGCGAACTCTTCTAATGAGACATGAAGCTGAACTCAAGTCCAATGCCTATTGGCTTAATCTATTAGCTCACTTGCAGGCCTCCTCTGTTCCGAGGAAGGAACTGTCATGCATTAAAGAACTTGTTTCATTGTATGAAGCTGCTTCGATCGAGGACATATACCTAGCTTATAATCAATTAAGAGTGGACGAGGACTCTCTGTACTCCTGTATTGGTATTGCTGGGGCACAGGCGGGTGAAGAGATTACTGTTCTCTCAGAAGAGGAAGAACCAGAAGATGTTTTCTCAGGAGTTGTTCCTGTGGGACGAGGCTCATCGATGACCACAAGACCAACAACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0007275 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M16 Peptidase_M16_C

Protein Analysis

1265

Amino Acids

140.61

Weight (kDa)

6.13

Isoelectric Point (pI)

45.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	218 - 342	6.5e-21	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	361 - 598	1.6e-14	Peptidase M16 inactive domain
Peptidase_M16_C	PF05193	928 - 1020	6e-11	Peptidase M16 inactive domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003778)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42390 AT5G42390
fragaria_vesca	FvH4_1g30291 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800
malus_domestica	MD00G1159400.v1.1 MD00G1204700.v1.1
prunus_persica	Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1
pyrus_communis	pycom290g00010
rosa_chinensis	RchiOBHm_Chr3g0491051
rosa_laevigata	RLG00000022866
rosa_multiflora	Rmu_co8044182.1_g000001 Rmu_ssc0000386.1_g000043
rosa_roxburghii	Rroxscaffold_5G00351210 Rroxscaffold_6G00393270
rosa_rugosa	Rorug03G0245500 Rorug04G0069600 Rorug04G0069700 Rorug04G0070700 Rorug04G0070800
rosa_samantha	Rh3AG295700 Rh3BG331900 Rh3CG329000 Rh3DG329500
rosa_wichuraiana	Rw3G026110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3627
AarI	CACCTGC	2 cut(s)	2342, 2467
AasI	GACNNNNNNGTC	1 cut(s)	2770
Acc36I	ACCTGC	3 cut(s)	1536, 2342, 2467
Acc65I	GGTACC	1 cut(s)	1310
AccB1I	GGYRCC	6 cut(s)	779, 1253, 1262, 1310, 1970, 2167
AccBSI	CCGCTC	2 cut(s)	84, 786
AccI	GTMKAC	1 cut(s)	3375
AccII	CGCG	2 cut(s)	139, 162
AccIII	TCCGGA	1 cut(s)	68
AclWI	GGATC	4 cut(s)	688, 1905, 2291, 2953
AcoI	YGGCCR	1 cut(s)	3231
AcsI	RAATTY	7 cut(s)	214, 914, 1103, 1468, 1748, 1985, 2549
AcuI	CTGAAG	2 cut(s)	950, 1448
AcyI	GRCGYC	1 cut(s)	1971
AdeI	CACNNNGTG	1 cut(s)	2608
AfaI	GTAC	7 cut(s)	466, 775, 1121, 1312, 2434, 2908, 3659
AfiI	CCNNNNNNNGG	8 cut(s)	191, 847, 1250, 1698, 2243, 2249, 2346, 2978
AflIII	ACRYGT	3 cut(s)	730, 1482, 1775
AgeI	ACCGGT	1 cut(s)	29
AjnI	CCWGG	6 cut(s)	1249, 1411, 1760, 1851, 2242, 2941
AjuI	GAANNNNNNNTTGG	4 cut(s)	537, 569, 1185, 1217
AleI	CACNNNNGTG	1 cut(s)	2054
Alw21I	GWGCWC	2 cut(s)	882, 2163
Alw26I	GTCTC	9 cut(s)	146, 490, 1518, 1910, 2270, 2454, 2562, 3159, 3469
AlwI	GGATC	4 cut(s)	688, 1905, 2291, 2953
AlwNI	CAGNNNCTG	1 cut(s)	2948
Ama87I	CYCGRG	4 cut(s)	927, 2162, 2346, 3332
Aor13HI	TCCGGA	1 cut(s)	68
AoxI	GGCC	4 cut(s)	473, 948, 3231, 3534
ApeKI	GCWGC	9 cut(s)	208, 458, 1291, 1817, 2027, 2387, 2596, 2656, 3596
ApoI	RAATTY	7 cut(s)	214, 914, 1103, 1468, 1748, 1985, 2549
ArsI	GACNNNNNNTTYG	6 cut(s)	1362, 1394, 1707, 1739, 3284, 3316
AseI	ATTAAT	1 cut(s)	1581
AsiGI	ACCGGT	1 cut(s)	29
Asp700I	GAANNNNTTC	1 cut(s)	549
Asp718I	GGTACC	1 cut(s)	1310
AspA2I	CCTAGG	1 cut(s)	3437
AspLEI	GCGC	3 cut(s)	208, 1973, 3052
AspS9I	GGNCC	4 cut(s)	440, 948, 1630, 3044
AsuC2I	CCSGG	6 cut(s)	268, 590, 1388, 2347, 2348, 3366
AsuHPI	GGTGA	8 cut(s)	19, 899, 1672, 1750, 1778, 2087, 2753, 3704
AsuII	TTCGAA	2 cut(s)	1576, 1735
AsuNHI	GCTAGC	1 cut(s)	2918
AvaI	CYCGRG	4 cut(s)	927, 2162, 2346, 3332
AvaII	GGWCC	3 cut(s)	440, 1630, 3044
AvrII	CCTAGG	1 cut(s)	3437
BaeGI	GKGCMC	3 cut(s)	784, 3196, 3685
BaeI	ACNNNNGTAYC	2 cut(s)	1582, 1615
BanI	GGYRCC	6 cut(s)	779, 1253, 1262, 1310, 1970, 2167
BanII	GRGCYC	2 cut(s)	1692, 2163
BauI	CACGAG	1 cut(s)	1454
BbsI	GAAGAC	4 cut(s)	1744, 1830, 2011, 3218
Bbv12I	GWGCWC	2 cut(s)	882, 2163
BbvI	GCAGC	9 cut(s)	220, 445, 1278, 1829, 2039, 2374, 2583, 2668, 3583
BccI	CCATC	7 cut(s)	136, 473, 880, 1110, 2060, 3054, 3119
BciT130I	CCWGG	6 cut(s)	1251, 1413, 1762, 1853, 2244, 2943
BciVI	GTATCC	1 cut(s)	3004
BclI	TGATCA	1 cut(s)	1342
BcnI	CCSGG	6 cut(s)	268, 590, 1388, 2347, 2348, 3366
BcoDI	GTCTC	9 cut(s)	146, 490, 1518, 1910, 2270, 2454, 2562, 3159, 3469
BfaI	CTAG	9 cut(s)	345, 542, 1245, 1982, 2792, 2919, 3155, 3438, 3620
BfmI	CTRYAG	3 cut(s)	1929, 2777, 2973
BfoI	RGCGCY	2 cut(s)	1974, 3053
BfuAI	ACCTGC	3 cut(s)	1536, 2342, 2467
BfuI	GTATCC	1 cut(s)	3004
BglII	AGATCT	1 cut(s)	502
BlnI	CCTAGG	1 cut(s)	3437
BlpI	GCTNAGC	1 cut(s)	2151
Bme18I	GGWCC	3 cut(s)	440, 1630, 3044
BmeT110I	CYCGRG	4 cut(s)	927, 2162, 2346, 3332
BmgT120I	GGNCC	4 cut(s)	440, 948, 1630, 3044
BmiI	GGNNCC	8 cut(s)	781, 1049, 1255, 1264, 1312, 1972, 2169, 3045
BmrI	ACTGGG	2 cut(s)	1230, 2289
BmsI	GCATC	8 cut(s)	874, 1646, 1777, 1864, 2626, 2797, 2898, 3379
BmtI	GCTAGC	1 cut(s)	2922
BmuI	ACTGGG	2 cut(s)	1230, 2289
BpiI	GAAGAC	4 cut(s)	1744, 1830, 2011, 3218
Bpu10I	CCTNAGC	1 cut(s)	2157
Bpu1102I	GCTNAGC	1 cut(s)	2151
Bpu14I	TTCGAA	2 cut(s)	1576, 1735
BpuEI	CTTGAG	4 cut(s)	267, 2633, 3232, 3476
BpuMI	CCSGG	6 cut(s)	268, 590, 1388, 2347, 2348, 3366
Bsa29I	ATCGAT	3 cut(s)	683, 3058, 3773
BsaHI	GRCGYC	1 cut(s)	1971
BsaI	GGTCTC	2 cut(s)	1518, 1910
BsaWI	WCCGGW	3 cut(s)	29, 68, 2553
BsaXI	ACNNNNNCTCC	4 cut(s)	2403, 2433, 3346, 3376
Bsc4I	CCNNNNNNNGG	8 cut(s)	191, 847, 1250, 1698, 2243, 2249, 2346, 2978
Bse118I	RCCGGY	1 cut(s)	29
Bse1I	ACTGG	4 cut(s)	1003, 1236, 1632, 2284
Bse3DI	GCAATG	2 cut(s)	2538, 3036
BseAI	TCCGGA	1 cut(s)	68
BseBI	CCWGG	6 cut(s)	1251, 1413, 1762, 1853, 2244, 2943
BseCI	ATCGAT	3 cut(s)	683, 3058, 3773
BseGI	GGATG	7 cut(s)	574, 865, 1090, 1661, 1792, 2889, 3088
BseLI	CCNNNNNNNGG	8 cut(s)	191, 847, 1250, 1698, 2243, 2249, 2346, 2978
BseMI	GCAATG	2 cut(s)	2538, 3036
BseNI	ACTGG	4 cut(s)	1003, 1236, 1632, 2284
BseRI	GAGGAG	5 cut(s)	138, 2546, 2885, 3177, 3529
BseSI	GKGCMC	3 cut(s)	784, 3196, 3685
BseXI	GCAGC	9 cut(s)	220, 445, 1278, 1829, 2039, 2374, 2583, 2668, 3583
BseYI	CCCAGC	3 cut(s)	1036, 1126, 3677
BsgI	GTGCAG	1 cut(s)	555
Bsh1236I	CGCG	2 cut(s)	139, 162
Bsh1285I	CGRYCG	2 cut(s)	2340, 3606
BshFI	GGCC	4 cut(s)	475, 950, 3233, 3536
BshNI	GGYRCC	6 cut(s)	779, 1253, 1262, 1310, 1970, 2167
BshTI	ACCGGT	1 cut(s)	29
BshVI	ATCGAT	3 cut(s)	683, 3058, 3773
BsiEI	CGRYCG	2 cut(s)	2340, 3606
BsiHKAI	GWGCWC	2 cut(s)	882, 2163
BsiHKCI	CYCGRG	4 cut(s)	927, 2162, 2346, 3332
BsiSI	CCGG	9 cut(s)	30, 69, 106, 267, 589, 1388, 2347, 2554, 3366
BslFI	GGGAC	7 cut(s)	240, 1368, 2219, 2846, 2873, 3057, 3774
BslI	CCNNNNNNNGG	8 cut(s)	191, 847, 1250, 1698, 2243, 2249, 2346, 2978
BsmAI	GTCTC	9 cut(s)	146, 490, 1518, 1910, 2270, 2454, 2562, 3159, 3469
BsmBI	CGTCTC	1 cut(s)	146
BsmFI	GGGAC	7 cut(s)	240, 1368, 2219, 2846, 2873, 3057, 3774
BsmI	GAATGC	2 cut(s)	433, 2583
BsnI	GGCC	4 cut(s)	475, 950, 3233, 3536
Bso31I	GGTCTC	2 cut(s)	1518, 1910
BsoBI	CYCGRG	4 cut(s)	927, 2162, 2346, 3332
Bsp119I	TTCGAA	2 cut(s)	1576, 1735
Bsp1286I	GDGCHC	6 cut(s)	784, 882, 1692, 2163, 3196, 3685
Bsp13I	TCCGGA	1 cut(s)	68
Bsp1720I	GCTNAGC	1 cut(s)	2151
Bsp19I	CCATGG	2 cut(s)	1266, 2544
BspANI	GGCC	4 cut(s)	475, 950, 3233, 3536
BspDI	ATCGAT	3 cut(s)	683, 3058, 3773
BspEI	TCCGGA	1 cut(s)	68
BspFNI	CGCG	2 cut(s)	139, 162
BspHI	TCATGA	3 cut(s)	1108, 2814, 3121
BspLI	GGNNCC	8 cut(s)	781, 1049, 1255, 1264, 1312, 1972, 2169, 3045
BspMI	ACCTGC	3 cut(s)	1536, 2342, 2467
BspOI	GCTAGC	1 cut(s)	2922
BspPI	GGATC	4 cut(s)	688, 1905, 2291, 2953
BspQI	GCTCTTC	3 cut(s)	328, 1059, 1797
BspT104I	TTCGAA	2 cut(s)	1576, 1735
BspT107I	GGYRCC	6 cut(s)	779, 1253, 1262, 1310, 1970, 2167
BspTNI	GGTCTC	2 cut(s)	1518, 1910
BsrBI	CCGCTC	2 cut(s)	84, 786
BsrDI	GCAATG	2 cut(s)	2538, 3036
BsrFI	RCCGGY	1 cut(s)	29
BsrI	ACTGG	4 cut(s)	1003, 1236, 1632, 2284
BssAI	RCCGGY	1 cut(s)	29
BssNAI	GTATAC	1 cut(s)	3376
BssNI	GRCGYC	1 cut(s)	1971
BssSI	CACGAG	1 cut(s)	1454
BssT1I	CCWWGG	7 cut(s)	742, 1266, 1920, 1974, 2544, 2902, 3437
Bst1107I	GTATAC	1 cut(s)	3376
Bst2BI	CACGAG	1 cut(s)	1454
Bst2UI	CCWGG	6 cut(s)	1251, 1413, 1762, 1853, 2244, 2943
BstACI	GRCGYC	1 cut(s)	1971
BstAPI	GCANNNNNTGC	2 cut(s)	2036, 3038
BstBI	TTCGAA	2 cut(s)	1576, 1735
BstC8I	GCNNGC	9 cut(s)	784, 948, 1296, 2583, 2703, 2920, 2980, 2984, 3534
BstDSI	CCRYGG	2 cut(s)	1266, 2544
BstENI	CCTNNNNNAGG	1 cut(s)	2247
BstF5I	GGATG	7 cut(s)	574, 865, 1090, 1661, 1792, 2889, 3088
BstFNI	CGCG	2 cut(s)	139, 162
BstH2I	RGCGCY	2 cut(s)	1974, 3053
BstHHI	GCGC	3 cut(s)	208, 1973, 3052
BstMAI	GTCTC	9 cut(s)	146, 490, 1518, 1910, 2270, 2454, 2562, 3159, 3469
BstMCI	CGRYCG	2 cut(s)	2340, 3606
BstNI	CCWGG	6 cut(s)	1251, 1413, 1762, 1853, 2244, 2943
BstNSI	RCATGY	6 cut(s)	458, 734, 1376, 1486, 2063, 3030
BstSFI	CTRYAG	3 cut(s)	1929, 2777, 2973
BstSLI	GKGCMC	3 cut(s)	784, 3196, 3685
BstUI	CGCG	2 cut(s)	139, 162
BstV1I	GCAGC	9 cut(s)	220, 445, 1278, 1829, 2039, 2374, 2583, 2668, 3583
BstV2I	GAAGAC	4 cut(s)	1744, 1830, 2011, 3218
BstX2I	RGATCY	2 cut(s)	502, 2945
BstXI	CCANNNNNNTGG	1 cut(s)	3060
BstYI	RGATCY	2 cut(s)	502, 2945
BstZ17I	GTATAC	1 cut(s)	3376
Bsu15I	ATCGAT	3 cut(s)	683, 3058, 3773
BsuI	GTATCC	1 cut(s)	3004
BsuRI	GGCC	4 cut(s)	475, 950, 3233, 3536
BsuTUI	ATCGAT	3 cut(s)	683, 3058, 3773
BtgI	CCRYGG	2 cut(s)	1266, 2544
BtgZI	GCGATG	1 cut(s)	1129
BtsCI	GGATG	7 cut(s)	574, 865, 1090, 1661, 1792, 2889, 3088
BtsI	GCAGTG	1 cut(s)	2010
BtsIMutI	CAGTG	3 cut(s)	1659, 1962, 2010
BveI	ACCTGC	3 cut(s)	1536, 2342, 2467
Cac8I	GCNNGC	9 cut(s)	784, 948, 1296, 2583, 2703, 2920, 2980, 2984, 3534
CaiI	CAGNNNCTG	1 cut(s)	2948
CciI	TCATGA	3 cut(s)	1108, 2814, 3121
CfoI	GCGC	3 cut(s)	208, 1973, 3052
Cfr10I	RCCGGY	1 cut(s)	29
Cfr13I	GGNCC	4 cut(s)	440, 948, 1630, 3044
Cfr9I	CCCGGG	1 cut(s)	2346
ClaI	ATCGAT	3 cut(s)	683, 3058, 3773
CseI	GACGC	3 cut(s)	128, 151, 1912
Csp6I	GTAC	7 cut(s)	465, 774, 1120, 1311, 2433, 2907, 3658
CspAI	ACCGGT	1 cut(s)	29
CviQI	GTAC	7 cut(s)	465, 774, 1120, 1311, 2433, 2907, 3658
DinI	GGCGCC	1 cut(s)	1972
DraI	TTTAAA	1 cut(s)	234
DraIII	CACNNNGTG	1 cut(s)	2608
DrdI	GACNNNNNNGTC	1 cut(s)	2770
DseDI	GACNNNNNNGTC	1 cut(s)	2770
EaeI	YGGCCR	1 cut(s)	3231
Ecl136II	GAGCTC	1 cut(s)	2161
Eco130I	CCWWGG	7 cut(s)	742, 1266, 1920, 1974, 2544, 2902, 3437
Eco147I	AGGCCT	1 cut(s)	3536
Eco24I	GRGCYC	2 cut(s)	1692, 2163
Eco31I	GGTCTC	2 cut(s)	1518, 1910
Eco32I	GATATC	4 cut(s)	92, 273, 617, 3348
Eco47I	GGWCC	3 cut(s)	440, 1630, 3044
Eco53kI	GAGCTC	1 cut(s)	2161
Eco57I	CTGAAG	2 cut(s)	950, 1448
Eco88I	CYCGRG	4 cut(s)	927, 2162, 2346, 3332
EcoICRI	GAGCTC	1 cut(s)	2161
EcoNI	CCTNNNNNAGG	1 cut(s)	2247
EcoRI	GAATTC	1 cut(s)	2549
EcoRII	CCWGG	6 cut(s)	1249, 1411, 1760, 1851, 2242, 2941
EcoRV	GATATC	4 cut(s)	92, 273, 617, 3348
EcoT14I	CCWWGG	7 cut(s)	742, 1266, 1920, 1974, 2544, 2902, 3437
EcoT22I	ATGCAT	2 cut(s)	2641, 3569
EcoT38I	GRGCYC	2 cut(s)	1692, 2163
EgeI	GGCGCC	1 cut(s)	1972
EheI	GGCGCC	1 cut(s)	1972
ErhI	CCWWGG	7 cut(s)	742, 1266, 1920, 1974, 2544, 2902, 3437
Esp3I	CGTCTC	1 cut(s)	146
FalI	AAGNNNNNCTT	2 cut(s)	537, 569
FaqI	GGGAC	7 cut(s)	240, 1368, 2219, 2846, 2873, 3057, 3774
FauI	CCCGC	5 cut(s)	791, 939, 2032, 3034, 3682
FauNDI	CATATG	2 cut(s)	720, 3289
FbaI	TGATCA	1 cut(s)	1342
FblI	GTMKAC	1 cut(s)	3375
FokI	GGATG	7 cut(s)	561, 872, 1097, 1668, 1799, 2876, 3095
FriOI	GRGCYC	2 cut(s)	1692, 2163
FspBI	CTAG	9 cut(s)	345, 542, 1245, 1982, 2792, 2919, 3155, 3438, 3620
GlaI	GCGC	3 cut(s)	207, 1972, 3051
GsaI	CCCAGC	3 cut(s)	1040, 1130, 3681
HaeII	RGCGCY	2 cut(s)	1974, 3053
HaeIII	GGCC	4 cut(s)	475, 950, 3233, 3536
HapII	CCGG	9 cut(s)	30, 69, 106, 267, 589, 1388, 2347, 2554, 3366
HgaI	GACGC	3 cut(s)	128, 151, 1912
HhaI	GCGC	3 cut(s)	208, 1973, 3052
Hin1I	GRCGYC	1 cut(s)	1971
Hin6I	GCGC	3 cut(s)	206, 1971, 3050
HinP1I	GCGC	3 cut(s)	206, 1971, 3050
HincII	GTYRAC	5 cut(s)	997, 1090, 2208, 2526, 3076
HindII	GTYRAC	5 cut(s)	997, 1090, 2208, 2526, 3076
HindIII	AAGCTT	2 cut(s)	2692, 3185
HpaI	GTTAAC	1 cut(s)	2208
HpaII	CCGG	9 cut(s)	30, 69, 106, 267, 589, 1388, 2347, 2554, 3366
HphI	GGTGA	8 cut(s)	19, 899, 1672, 1750, 1778, 2087, 2753, 3704
Hpy99I	CGWCG	2 cut(s)	136, 167
HpyAV	CCTTC	7 cut(s)	579, 841, 1267, 1641, 1646, 3001, 3547
HpyCH4IV	ACGT	6 cut(s)	229, 289, 757, 1616, 1777, 3294
HpySE526I	ACGT	6 cut(s)	229, 289, 757, 1616, 1777, 3294
Hsp92I	GRCGYC	1 cut(s)	1971
HspAI	GCGC	3 cut(s)	206, 1971, 3050
KasI	GGCGCC	1 cut(s)	1970
Kpn2I	TCCGGA	1 cut(s)	68
KpnI	GGTACC	1 cut(s)	1314
Ksp22I	TGATCA	1 cut(s)	1342
KspAI	GTTAAC	1 cut(s)	2208
LguI	GCTCTTC	3 cut(s)	328, 1059, 1797
LmnI	GCTCC	7 cut(s)	61, 175, 747, 2411, 2473, 2482, 2698
Lsp1109I	GCAGC	9 cut(s)	220, 445, 1278, 1829, 2039, 2374, 2583, 2668, 3583
LweI	GCATC	8 cut(s)	874, 1646, 1777, 1864, 2626, 2797, 2898, 3379
MaeI	CTAG	9 cut(s)	345, 542, 1245, 1982, 2792, 2919, 3155, 3438, 3620
MaeII	ACGT	6 cut(s)	229, 289, 757, 1616, 1777, 3294
MaeIII	GTNAC	8 cut(s)	1663, 1678, 1756, 1778, 1942, 2772, 3214, 3352
MbiI	CCGCTC	2 cut(s)	84, 786
MfeI	CAATTG	4 cut(s)	596, 981, 2195, 3149
MflI	RGATCY	2 cut(s)	502, 2945
MhlI	GDGCHC	6 cut(s)	784, 882, 1692, 2163, 3196, 3685
Mly113I	GGCGCC	1 cut(s)	1971
MlyI	GAGTC	6 cut(s)	845, 1663, 2123, 2531, 2827, 3644
MmeI	TCCRAC	5 cut(s)	860, 2437, 2499, 2610, 3426
Mph1103I	ATGCAT	2 cut(s)	2641, 3569
MroI	TCCGGA	1 cut(s)	68
MroXI	GAANNNNTTC	1 cut(s)	549
MslI	CAYNNNNRTG	4 cut(s)	1892, 2054, 2064, 2543
MspA1I	CMGCKG	3 cut(s)	1298, 2596, 2978
MspI	CCGG	9 cut(s)	30, 69, 106, 267, 589, 1388, 2347, 2554, 3366
MunI	CAATTG	4 cut(s)	596, 981, 2195, 3149
Mva1269I	GAATGC	2 cut(s)	433, 2583
MvaI	CCWGG	6 cut(s)	1251, 1413, 1762, 1853, 2244, 2943
MvnI	CGCG	2 cut(s)	139, 162
NarI	GGCGCC	1 cut(s)	1971
NciI	CCSGG	6 cut(s)	268, 590, 1388, 2347, 2348, 3366
NcoI	CCATGG	2 cut(s)	1266, 2544
NdeI	CATATG	2 cut(s)	720, 3289
NheI	GCTAGC	1 cut(s)	2918
NlaIV	GGNNCC	8 cut(s)	781, 1049, 1255, 1264, 1312, 1972, 2169, 3045
NmuCI	GTSAC	4 cut(s)	1678, 1756, 2772, 3352
NsiI	ATGCAT	2 cut(s)	2641, 3569
NspI	RCATGY	6 cut(s)	458, 734, 1376, 1486, 2063, 3030
NspV	TTCGAA	2 cut(s)	1576, 1735
OliI	CACNNNNGTG	1 cut(s)	2054
PaeR7I	CTCGAG	2 cut(s)	927, 2162
PagI	TCATGA	3 cut(s)	1108, 2814, 3121
PaqCI	CACCTGC	2 cut(s)	2342, 2467
PceI	AGGCCT	1 cut(s)	3536
PciI	ACATGT	2 cut(s)	730, 1482
PciSI	GCTCTTC	3 cut(s)	328, 1059, 1797
PctI	GAATGC	2 cut(s)	433, 2583
PdmI	GAANNNNTTC	1 cut(s)	549
PfoI	TCCNGGA	1 cut(s)	1411
PinAI	ACCGGT	1 cut(s)	29
Ple19I	CGATCG	1 cut(s)	3606
PleI	GAGTC	6 cut(s)	845, 1663, 2122, 2530, 2826, 3644
PluTI	GGCGCC	1 cut(s)	1974
PpsI	GAGTC	6 cut(s)	845, 1663, 2122, 2530, 2826, 3644
PscI	ACATGT	2 cut(s)	730, 1482
PshBI	ATTAAT	1 cut(s)	1581
PsiI	TTATAA	1 cut(s)	3627
Psp124BI	GAGCTC	1 cut(s)	2163
Psp6I	CCWGG	6 cut(s)	1249, 1411, 1760, 1851, 2242, 2941
PspFI	CCCAGC	3 cut(s)	1036, 1126, 3677
PspGI	CCWGG	6 cut(s)	1249, 1411, 1760, 1851, 2242, 2941
PspN4I	GGNNCC	8 cut(s)	781, 1049, 1255, 1264, 1312, 1972, 2169, 3045
PspPI	GGNCC	4 cut(s)	440, 948, 1630, 3044
PspXI	VCTCGAGB	1 cut(s)	2162
PstNI	CAGNNNCTG	1 cut(s)	2948
PsuI	RGATCY	2 cut(s)	502, 2945
PvuI	CGATCG	1 cut(s)	3606
PvuII	CAGCTG	3 cut(s)	1298, 2596, 2978
RsaI	GTAC	7 cut(s)	466, 775, 1121, 1312, 2434, 2908, 3659
RsaNI	GTAC	7 cut(s)	465, 774, 1120, 1311, 2433, 2907, 3658
RseI	CAYNNNNRTG	4 cut(s)	1892, 2054, 2064, 2543
SacI	GAGCTC	1 cut(s)	2163
SapI	GCTCTTC	3 cut(s)	328, 1059, 1797
Sau96I	GGNCC	4 cut(s)	440, 948, 1630, 3044
SchI	GAGTC	6 cut(s)	845, 1663, 2123, 2531, 2827, 3644
SduI	GDGCHC	6 cut(s)	784, 882, 1692, 2163, 3196, 3685
SfaNI	GCATC	8 cut(s)	874, 1646, 1777, 1864, 2626, 2797, 2898, 3379
SfcI	CTRYAG	3 cut(s)	1929, 2777, 2973
SfoI	GGCGCC	1 cut(s)	1972
Sfr274I	CTCGAG	2 cut(s)	927, 2162
SfuI	TTCGAA	2 cut(s)	1576, 1735
SgrAI	CRCCGGYG	1 cut(s)	29
SinI	GGWCC	3 cut(s)	440, 1630, 3044
SlaI	CTCGAG	2 cut(s)	927, 2162
SmaI	CCCGGG	1 cut(s)	2348
SmiMI	CAYNNNNRTG	4 cut(s)	1892, 2054, 2064, 2543
SmlI	CTYRAG	6 cut(s)	282, 927, 2162, 2612, 3247, 3491
SmoI	CTYRAG	6 cut(s)	282, 927, 2162, 2612, 3247, 3491
SseBI	AGGCCT	1 cut(s)	3536
SspDI	GGCGCC	1 cut(s)	1970
SspI	AATATT	2 cut(s)	1189, 1554
SspMI	CTAG	9 cut(s)	345, 542, 1245, 1982, 2792, 2919, 3155, 3438, 3620
SstI	GAGCTC	1 cut(s)	2163
StuI	AGGCCT	1 cut(s)	3536
StyI	CCWWGG	7 cut(s)	742, 1266, 1920, 1974, 2544, 2902, 3437
TaiI	ACGT	6 cut(s)	232, 292, 760, 1619, 1780, 3297
TaqII	GACCGA	1 cut(s)	2354
TatI	WGTACW	1 cut(s)	3657
TauI	GCSGC	6 cut(s)	81, 84, 139, 206, 2365, 2588
TscAI	CASTG	3 cut(s)	1666, 1969, 2017
TseFI	GTSAC	4 cut(s)	1678, 1756, 2772, 3352
TseI	GCWGC	9 cut(s)	208, 458, 1291, 1817, 2027, 2387, 2596, 2656, 3596
Tsp45I	GTSAC	4 cut(s)	1678, 1756, 2772, 3352
TspGWI	ACGGA	1 cut(s)	162
TspMI	CCCGGG	1 cut(s)	2346
TspRI	CASTG	3 cut(s)	1666, 1969, 2017
VpaK11BI	GGWCC	3 cut(s)	440, 1630, 3044
VspI	ATTAAT	1 cut(s)	1581
XagI	CCTNNNNNAGG	1 cut(s)	2247
XapI	RAATTY	7 cut(s)	214, 914, 1103, 1468, 1748, 1985, 2549
XbaI	TCTAGA	1 cut(s)	2791
XceI	RCATGY	6 cut(s)	458, 734, 1376, 1486, 2063, 3030
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2413, 2975
XhoI	CTCGAG	2 cut(s)	927, 2162
XmaI	CCCGGG	1 cut(s)	2346
XmaJI	CCTAGG	1 cut(s)	3437
XmiI	GTMKAC	1 cut(s)	3375
XmnI	GAANNNNTTC	1 cut(s)	549
XspI	CTAG	9 cut(s)	345, 542, 1245, 1982, 2792, 2919, 3155, 3438, 3620
Zsp2I	ATGCAT	2 cut(s)	2641, 3569

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.