Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0003778

Physical Location & Seq

Genomic Coordinates Forward (+)

34889502 .. 34903968

Gene Structure 14467 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG331900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3495 bp

ATGGCGGCTATAAGTTCTCTGGTGGGAAGCATTCCCCAAATTGGTCCGACAATATGCAGAGATGATGGTTATTCAAAAAGGCGGGCGAAGAATTCTGTGGTTTCTATTAGGGGCCCTCGTCTACCCTTATTTTCTTCTCAGACCAGACGTCTGCTTCAAGTTGGTCCGGGTAAATCAAGTTTACCAAGAAAGAAGAGGAATAGTTGGGAACAATGTATCTCCATATTTGGAGAGCCTTTGACTGGAGCACCTTTTCCCCGAAAGTATAACGGCATTTCTAGCTCTTTTTGCCAAAGCAGAAGCTCTTCATGGCAAAGTAGAAGTCGTCTTCGCCTCAGGACGTCCACACCAAGTGCCTTTCCTGACAGAACTTCTTTCTGTTTATCCAACCGTAAACTTGAGAAGGCTTTTGTGAAGGATCTGCATATTCCATATGCAACCGTAGGTCCAGATGAGCCACATGCAGCAAGTACATCTTGGCCAGATGGTATTCTTGAGAAACAGGAGCCAGATTTAGTATATCCTGGAGTAGAACAAACAGAGGTAGAGGGGTTTCTAAGTTCTGAACTTCCATCTCACCCAAAGTTGTATCGAGGACAACTAAAAAATGGTCTGCGCTATCTGATTTTACCAAATAAAGTTCCGCCAAACAGGTTTGAAGCACACATGGAAGTGCATGTAGGATCAATCGACGAGGAAGAGGATGAGCAAGGGATTGCACATATGATTGAACATGTTGCTTTCCTTGGAAGTAAGAAACGCGAGAAACTTCTTGGGACGGGGGCTCGATCCAATGCTTACACCGATTTCCATCATACAGTGTTTCACATCCATTCGCCACTTAGTACAAAGGATTCTGATGAAGATCTCCTTCCAAATGTGCTGGATGCCTTGAATGAGATTGCTTTTCACCCAAAATTCCTTTCTTCTCGTGTTGAGAAGGAACGGCGGGCTATACTTTCAGAATTACAGATGATGAATACAATAGATTATCGCGTTGACTGCCAGTTATTACAACATCTGCACTCTGAAAACAAGCTAAGCAAAAGGTTCCCGATTGGATTAGAGGAGCAGATTAAGAAGTGGGATGTAGATAAAGTGCGAAAATTTCATGAGCGTTGGTACTTCCCTGCAAATGCAACCTTATACATTGTTGGGGATATTGACAACATCTCAAAAACAATTTACCAAATTGAGGCTGTCTTTGGACAAACTGGCCAAGAAAATGGGTCAGCACCTACTCCTAGTGCGTTTGGTGCAATGGCTAGTTTCCTTGTCCCTAAGCTTTCAGTTGGCCTGACTGGAAATTTGCCTACTGAAAGATCGTCTAATTCCATTGATCAAACAAAACTCCTTAAAAAGGAAAAGCACACTGTTCGTCCTCCTGTGAAGCATAATTGGTCACTCCCTGGAAGTAGTATGCATTTGAAGCCTCCACAAATTTTTCAGCATGAATTGATTCAGAATTTCTCATTTAATATGTTCTGCAAGATTCCTGTGAACAGGGTTCGAACATATGGCGACTTGCGAAATGTTCTAATGAAGAGAATATTTCTTTCTGCTTTGCATTTCCGAATTAATACAAGATACAAGAGTTCAAACCCACCATTTACTTCAATTGAATTGGATCATAGTGACTCTGGAAGGGAAGGATGCACAGTCACTACTCTCACTGTAACTGCCGAACCCAAGAATTGGCAAACTGCAATAAGAGTTGCTGTTCACGAGGTTAGAAGGCTCAAAGAATTTGGTGTTACTAAGGGTGAATTGACACGTTATATAGATGCTTTATTAAAAGATAGTGAGCATCTGGCGGCTATGATAGATAATGTATCTTCAGTTGATAATTTGGATTTCATTATGGAGAGTGATGCGCTGGGCCATACAGTAATGGATCAGAGACAAGGACATGACAGTCTGGTTGCTGTTGCTGGAACTGTTACTCTTGAAGAGGTTAACTCTATTGGCGCCAAGGTGTTGGAATTTGTTTCTGATTTTGGAAAGCCTACTGCACCCCTTCCTGCAGCGATCGTTGCTTGTGTTCCAAAGAAAGTGCATGTTGATGGAAAGGGTGAAACTGAGTTCACCATTTCCCCTGATGAGATTACAGCTGCTACGAGAGCTGGATTGGAGGACCTCATTGAACCTGAGCCAGAGCTTGAAGTGCCAAAAGAATTGATATCTTCATCACAGTTGCAGGAATTGAGGCAGGAGCGCATGCCATCCTTCATCACCTGTAGCCCAGAAACAAATATGACAAAAATTTATGACAAGGAAACAGGGATCACTAGAGCTCGTCTTTCAAATGGAATTGCTGTAAATTACAAGATTTCAAAAAGTGAGGCTCGGGGAGGTGTGATGAGGCTTATAGTTGGTGGTGGAAGGGCAACTGAAAGTCCTGAATCAAAAGGTTCTGTTGTTGTGGGTGTACGAACTCTTAGCGAGGGAGGTCGTGTTGGAAACTTTTCAAGGGAGCAGGTAGAACTGTTCTGTGTGAATCACTTGATTAATTGCTCATTGGAGTCCACGGAGGAATTTATTTCTATGGAATTCCGTTTCACTTTAAGAGACAATGGGATGCGTGCCGCTTTCCAACTGCTTCACATGGTACTTGAGCATAGTGTCTGGCTGGATGATGCATTTGATAGAGCAAGACAATTATATTTGTCATATTATCGCTCCATCCCTAAAAGCTTGGAGCGCTCAACTGCTTACAAACTCATGTTGGCAATGTTGGACGGGGATGAGCGTTTTGTAGAACCTACACCAACATCATTGCAAAATTTAACTTTGCAATCTGTGAAGGATGCAGTGATGAACCAATTTGTTGGCAATAACATGGAGGTAAGTGTTGTTGGGGACTTCTCGGAGGAGGAAATCGAGTCTTGTATTCTTGATTACTTGGGAACAGTACAATCAGCCAAAAAATTTGAGACTGAGCAGAAGTATAATCCGGTTGTGTTTCGACCATCTTCTGATTTGCAGTCTCAGCAAGTGTTCTTGAAAGATACAGATGAGAGAGCTTGTGCATATATTGCAGGTCCTGCACCAAATCGTTGGGGTTTTACTGTTGACAGTAAAGACCTGTTTGAGTCGATCACTGATATTTCTTCTAGTGATGATGCGCAGCTGAAATCTGCAGAGCTAGTTGTGGAGGGTAAGGATATTCAGAGGGATCTGCAAAGAAAACTCCGTGGTCATCCGCTTTTCTTTGGCATCACAATGGGGCTCCTGGCTGAGATTATAAATTCCAGGCTGAATCTCGGATGGTATGTCATATCTGTAACATCAACTCCTGGCAAGGTTCACAAAGCTGTTGATGCATGCAAGAATGTCCTTAGAGGTTTGCATAGCAACAAGATTTCCCAAAGGGAGCTGGACAGGGCAAAACGGACGCTTCTGATGAGACATGAAGCTGAGATCAAGTCAAATGGCTACTGGCTTGGACTTTTAGCTCACTTGCAAGCTTCTTCTGTTCCAAGGAAGGTAAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0007275 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M16 Peptidase_M16_C

Protein Analysis

1164

Amino Acids

130.5

Weight (kDa)

7.64

Isoelectric Point (pI)

44.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	219 - 343	1.2e-20	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	362 - 597	1.8e-12	Peptidase M16 inactive domain
Peptidase_M16_C	PF05193	928 - 1133	6.8e-18	Peptidase M16 inactive domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003778)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42390 AT5G42390
fragaria_vesca	FvH4_1g30291 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800
malus_domestica	MD00G1159400.v1.1 MD00G1204700.v1.1
prunus_persica	Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1
pyrus_communis	pycom290g00010
rosa_chinensis	RchiOBHm_Chr3g0491051
rosa_laevigata	RLG00000022866
rosa_multiflora	Rmu_co8044182.1_g000001 Rmu_ssc0000386.1_g000043
rosa_roxburghii	Rroxscaffold_5G00351210 Rroxscaffold_6G00393270
rosa_rugosa	Rorug03G0245500 Rorug04G0069600 Rorug04G0069700 Rorug04G0070700 Rorug04G0070800
rosa_samantha	Rh3AG295700 Rh3BG331900 Rh3CG329000 Rh3DG329500
rosa_wichuraiana	Rw3G026110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3245
AasI	GACNNNNNNGTC	1 cut(s)	1914
AatII	GACGTC	2 cut(s)	151, 344
Acc16I	TGCGCA	1 cut(s)	3126
Acc36I	ACCTGC	2 cut(s)	2467, 3029
AccB1I	GGYRCC	1 cut(s)	1967
AccB7I	CCANNNNNTGG	1 cut(s)	1695
AccI	GTMKAC	1 cut(s)	121
AccII	CGCG	2 cut(s)	762, 996
AciI	CCGC	7 cut(s)	5, 82, 644, 949, 1814, 2586, 3203
AclWI	GGATC	7 cut(s)	426, 691, 783, 1635, 1902, 2291, 3183
AcoI	YGGCCR	2 cut(s)	479, 1216
AcuI	CTGAAG	1 cut(s)	1821
AcyI	GRCGYC	3 cut(s)	148, 341, 1968
AdeI	CACNNNGTG	1 cut(s)	353
AfaI	GTAC	6 cut(s)	472, 847, 1124, 2430, 2610, 2913
AfeI	AGCGCT	1 cut(s)	2702
AfiI	CCNNNNNNNGG	5 cut(s)	41, 242, 1195, 1360, 1695
AflIII	ACRYGT	2 cut(s)	733, 1772
AhdI	GACNNNNNGTC	1 cut(s)	147
AjnI	CCWGG	5 cut(s)	523, 1408, 3231, 3251, 3295
AjuI	GAANNNNNNNTTGG	2 cut(s)	907, 939
Alw21I	GWGCWC	2 cut(s)	250, 2296
Alw26I	GTCTC	5 cut(s)	1894, 2562, 2927, 2991, 3400
AlwI	GGATC	7 cut(s)	426, 691, 783, 1635, 1902, 2291, 3183
AlwNI	CAGNNNCTG	1 cut(s)	3044
Ama87I	CYCGRG	1 cut(s)	2346
Aor51HI	AGCGCT	1 cut(s)	2702
AoxI	GGCC	5 cut(s)	112, 479, 1216, 1294, 1879
ApaI	GGGCCC	1 cut(s)	116
ApeKI	GCWGC	4 cut(s)	464, 2024, 2111, 3127
ArsI	GACNNNNNNTTYG	4 cut(s)	307, 339, 1291, 1323
AseI	ATTAAT	2 cut(s)	1578, 2508
Asp700I	GAANNNNTTC	3 cut(s)	304, 1458, 2464
AspLEI	GCGC	6 cut(s)	618, 1876, 1970, 2217, 2703, 3127
AspS9I	GGNCC	8 cut(s)	44, 112, 113, 164, 446, 1879, 2134, 3041
AsuC2I	CCSGG	1 cut(s)	168
AsuHPI	GGTGA	6 cut(s)	569, 902, 1775, 2077, 2084, 2224
AsuII	TTCGAA	1 cut(s)	1510
AvaI	CYCGRG	1 cut(s)	2346
AvaII	GGWCC	5 cut(s)	44, 164, 446, 2134, 3041
AxyI	CCTNAGG	1 cut(s)	335
BaeGI	GKGCMC	1 cut(s)	116
BaeI	ACNNNNGTAYC	2 cut(s)	1579, 1612
BalI	TGGCCA	2 cut(s)	481, 1218
BanI	GGYRCC	1 cut(s)	1967
BanII	GRGCYC	4 cut(s)	116, 787, 2296, 3231
BauI	CACGAG	2 cut(s)	930, 1724
BbsI	GAAGAC	1 cut(s)	320
Bbv12I	GWGCWC	2 cut(s)	250, 2296
BbvI	GCAGC	4 cut(s)	476, 2036, 2098, 3139
BccI	CCATC	9 cut(s)	59, 479, 580, 819, 2057, 2230, 2690, 2977, 3261
BceAI	ACGGC	2 cut(s)	286, 962
BcgI	CGANNNNNNTGC	2 cut(s)	1358, 1392
BciT130I	CCWGG	5 cut(s)	525, 1410, 3233, 3253, 3297
BclI	TGATCA	1 cut(s)	1339
BcnI	CCSGG	1 cut(s)	168
BcoDI	GTCTC	5 cut(s)	1894, 2562, 2927, 2991, 3400
BfaI	CTAG	7 cut(s)	279, 1245, 1266, 2289, 3114, 3146, 3493
BfmI	CTRYAG	3 cut(s)	2022, 2236, 3138
BfoI	RGCGCY	2 cut(s)	1971, 2704
BfuAI	ACCTGC	2 cut(s)	2467, 3029
BglII	AGATCT	1 cut(s)	865
BisI	GCNGC	7 cut(s)	6, 465, 1815, 2025, 2112, 2586, 3128
BlpI	GCTNAGC	1 cut(s)	1040
BlsI	GCNGC	7 cut(s)	7, 466, 1816, 2026, 2113, 2587, 3129
Bme1390I	CCNGG	6 cut(s)	168, 525, 1410, 3233, 3253, 3297
Bme18I	GGWCC	5 cut(s)	44, 164, 446, 2134, 3041
BmeRI	GACNNNNNGTC	1 cut(s)	147
BmeT110I	CYCGRG	1 cut(s)	2346
BmgT120I	GGNCC	8 cut(s)	44, 112, 113, 164, 446, 1879, 2134, 3041
BmiI	GGNNCC	6 cut(s)	113, 114, 507, 1052, 1969, 3230
BmrFI	CCNGG	6 cut(s)	168, 525, 1410, 3233, 3253, 3297
BpiI	GAAGAC	1 cut(s)	320
BplI	GAGNNNNNCTC	2 cut(s)	1943, 1975
BpmI	CTGGAG	2 cut(s)	264, 546
Bpu10I	CCTNAGC	2 cut(s)	1281, 2148
Bpu1102I	GCTNAGC	1 cut(s)	1040
Bpu14I	TTCGAA	1 cut(s)	1510
BpuEI	CTTGAG	3 cut(s)	419, 515, 2633
BpuMI	CCSGG	1 cut(s)	168
BsaBI	GATNNNNATC	2 cut(s)	810, 864
BsaHI	GRCGYC	3 cut(s)	148, 341, 1968
BsaJI	CCNNGG	6 cut(s)	745, 1408, 1971, 2526, 3193, 3479
BsaWI	WCCGGW	1 cut(s)	2953
BsaXI	ACNNNNNCTCC	4 cut(s)	2439, 2469, 3277, 3307
Bsc4I	CCNNNNNNNGG	5 cut(s)	41, 242, 1195, 1360, 1695
Bse1I	ACTGG	5 cut(s)	247, 1006, 1219, 1306, 3443
Bse21I	CCTNAGG	1 cut(s)	335
Bse3DI	GCAATG	3 cut(s)	1266, 2736, 2774
Bse8I	GATNNNNATC	2 cut(s)	810, 864
BseBI	CCWGG	5 cut(s)	525, 1410, 3233, 3253, 3297
BseDI	CCNNGG	6 cut(s)	745, 1408, 1971, 2526, 3193, 3479
BseJI	GATNNNNATC	2 cut(s)	810, 864
BseLI	CCNNNNNNNGG	5 cut(s)	41, 242, 1195, 1360, 1695
BseMI	GCAATG	3 cut(s)	1266, 2736, 2774
BseMII	CTCAG	8 cut(s)	152, 349, 2070, 2139, 2928, 3002, 3228, 3408
BseNI	ACTGG	5 cut(s)	247, 1006, 1219, 1306, 3443
BseRI	GAGGAG	2 cut(s)	1082, 2885
BseSI	GKGCMC	1 cut(s)	116
BseXI	GCAGC	4 cut(s)	476, 2036, 2098, 3139
BseYI	CCCAGC	1 cut(s)	1876
BsgI	GTGCAG	3 cut(s)	1007, 1995, 3030
Bsh1236I	CGCG	2 cut(s)	762, 996
Bsh1285I	CGRYCG	1 cut(s)	2031
BshFI	GGCC	5 cut(s)	114, 481, 1218, 1296, 1881
BshNI	GGYRCC	1 cut(s)	1967
BsiEI	CGRYCG	1 cut(s)	2031
BsiHKAI	GWGCWC	2 cut(s)	250, 2296
BsiHKCI	CYCGRG	1 cut(s)	2346
BsiSI	CCGG	2 cut(s)	167, 2954
BslFI	GGGAC	3 cut(s)	790, 1262, 2873
BslI	CCNNNNNNNGG	5 cut(s)	41, 242, 1195, 1360, 1695
BsmAI	GTCTC	5 cut(s)	1894, 2562, 2927, 2991, 3400
BsmFI	GGGAC	3 cut(s)	790, 1262, 2873
BsmI	GAATGC	1 cut(s)	30
BsnI	GGCC	5 cut(s)	114, 481, 1218, 1296, 1881
BsoBI	CYCGRG	1 cut(s)	2346
Bsp119I	TTCGAA	1 cut(s)	1510
Bsp120I	GGGCCC	1 cut(s)	112
Bsp1286I	GDGCHC	5 cut(s)	116, 250, 787, 2296, 3231
Bsp1720I	GCTNAGC	1 cut(s)	1040
BspACI	CCGC	7 cut(s)	5, 82, 644, 949, 1814, 2586, 3203
BspANI	GGCC	5 cut(s)	114, 481, 1218, 1296, 1881
BspCNI	CTCAG	8 cut(s)	151, 348, 2071, 2140, 2929, 3001, 3229, 3409
BspFNI	CGCG	2 cut(s)	762, 996
BspHI	TCATGA	1 cut(s)	1111
BspLI	GGNNCC	6 cut(s)	113, 114, 507, 1052, 1969, 3230
BspMAI	CTGCAG	2 cut(s)	2026, 3142
BspMI	ACCTGC	2 cut(s)	2467, 3029
BspPI	GGATC	7 cut(s)	426, 691, 783, 1635, 1902, 2291, 3183
BspQI	GCTCTTC	1 cut(s)	310
BspT104I	TTCGAA	1 cut(s)	1510
BspT107I	GGYRCC	1 cut(s)	1967
BsrDI	GCAATG	3 cut(s)	1266, 2736, 2774
BsrI	ACTGG	5 cut(s)	247, 1006, 1219, 1306, 3443
BssECI	CCNNGG	6 cut(s)	745, 1408, 1971, 2526, 3193, 3479
BssNI	GRCGYC	3 cut(s)	148, 341, 1968
BssSI	CACGAG	2 cut(s)	930, 1724
BssT1I	CCWWGG	3 cut(s)	745, 1971, 3479
Bst2BI	CACGAG	2 cut(s)	930, 1724
Bst2UI	CCWGG	5 cut(s)	525, 1410, 3233, 3253, 3297
Bst6I	CTCTTC	5 cut(s)	188, 310, 693, 1538, 1944
BstACI	GRCGYC	3 cut(s)	148, 341, 1968
BstAPI	GCANNNNNTGC	2 cut(s)	3035, 3044
BstBI	TTCGAA	1 cut(s)	1510
BstC8I	GCNNGC	6 cut(s)	84, 951, 2219, 2583, 3325, 3465
BstDSI	CCRYGG	2 cut(s)	2526, 3193
BstENI	CCTNNNNNAGG	1 cut(s)	1358
BstFNI	CGCG	2 cut(s)	762, 996
BstH2I	RGCGCY	2 cut(s)	1971, 2704
BstHHI	GCGC	6 cut(s)	618, 1876, 1970, 2217, 2703, 3127
BstMAI	GTCTC	5 cut(s)	1894, 2562, 2927, 2991, 3400
BstMCI	CGRYCG	1 cut(s)	2031
BstNI	CCWGG	5 cut(s)	525, 1410, 3233, 3253, 3297
BstNSI	RCATGY	6 cut(s)	464, 680, 737, 2060, 2221, 3327
BstSCI	CCNGG	6 cut(s)	166, 523, 1408, 3231, 3251, 3295
BstSFI	CTRYAG	3 cut(s)	2022, 2236, 3138
BstSLI	GKGCMC	1 cut(s)	116
BstUI	CGCG	2 cut(s)	762, 996
BstV1I	GCAGC	4 cut(s)	476, 2036, 2098, 3139
BstV2I	GAAGAC	1 cut(s)	320
BstX2I	RGATCY	3 cut(s)	418, 865, 3175
BstXI	CCANNNNNNTGG	4 cut(s)	1226, 1978, 2828, 3057
BstYI	RGATCY	3 cut(s)	418, 865, 3175
Bsu36I	CCTNAGG	1 cut(s)	335
BsuRI	GGCC	5 cut(s)	114, 481, 1218, 1296, 1881
BtgI	CCRYGG	2 cut(s)	2526, 3193
BtsI	GCAGTG	1 cut(s)	2817
BtsIMutI	CAGTG	5 cut(s)	825, 1371, 1671, 2817, 3099
BveI	ACCTGC	2 cut(s)	2467, 3029
Cac8I	GCNNGC	6 cut(s)	84, 951, 2219, 2583, 3325, 3465
CaiI	CAGNNNCTG	1 cut(s)	3044
CciI	TCATGA	1 cut(s)	1111
CfoI	GCGC	6 cut(s)	618, 1876, 1970, 2217, 2703, 3127
Cfr13I	GGNCC	8 cut(s)	44, 112, 113, 164, 446, 1879, 2134, 3041
CseI	GACGC	1 cut(s)	3403
Csp6I	GTAC	6 cut(s)	471, 846, 1123, 2429, 2609, 2912
CviQI	GTAC	6 cut(s)	471, 846, 1123, 2429, 2609, 2912
DinI	GGCGCC	1 cut(s)	1969
DraIII	CACNNNGTG	1 cut(s)	353
DrdI	GACNNNNNNGTC	1 cut(s)	1914
DriI	GACNNNNNGTC	1 cut(s)	147
DseDI	GACNNNNNNGTC	1 cut(s)	1914
EaeI	YGGCCR	2 cut(s)	479, 1216
Eam1104I	CTCTTC	5 cut(s)	188, 310, 693, 1538, 1944
Eam1105I	GACNNNNNGTC	1 cut(s)	147
EarI	CTCTTC	5 cut(s)	188, 310, 693, 1538, 1944
EciI	GGCGGA	1 cut(s)	633
Ecl136II	GAGCTC	1 cut(s)	2294
Eco130I	CCWWGG	3 cut(s)	745, 1971, 3479
Eco24I	GRGCYC	4 cut(s)	116, 787, 2296, 3231
Eco32I	GATATC	1 cut(s)	2181
Eco47I	GGWCC	5 cut(s)	44, 164, 446, 2134, 3041
Eco47III	AGCGCT	1 cut(s)	2702
Eco53kI	GAGCTC	1 cut(s)	2294
Eco57I	CTGAAG	1 cut(s)	1821
Eco81I	CCTNAGG	1 cut(s)	335
Eco88I	CYCGRG	1 cut(s)	2346
EcoICRI	GAGCTC	1 cut(s)	2294
EcoNI	CCTNNNNNAGG	1 cut(s)	1358
EcoO109I	RGGNCCY	4 cut(s)	112, 113, 2134, 3041
EcoRI	GAATTC	2 cut(s)	91, 2549
EcoRII	CCWGG	5 cut(s)	523, 1408, 3231, 3251, 3295
EcoRV	GATATC	1 cut(s)	2181
EcoT14I	CCWWGG	3 cut(s)	745, 1971, 3479
EcoT22I	ATGCAT	3 cut(s)	1425, 2641, 3325
EcoT38I	GRGCYC	4 cut(s)	116, 787, 2296, 3231
EgeI	GGCGCC	1 cut(s)	1969
EheI	GGCGCC	1 cut(s)	1969
ErhI	CCWWGG	3 cut(s)	745, 1971, 3479
FalI	AAGNNNNNCTT	4 cut(s)	460, 492, 855, 887
FaqI	GGGAC	3 cut(s)	790, 1262, 2873
FauI	CCCGC	2 cut(s)	75, 942
FauNDI	CATATG	3 cut(s)	433, 723, 1516
FbaI	TGATCA	1 cut(s)	1339
FblI	GTMKAC	1 cut(s)	121
Fnu4HI	GCNGC	7 cut(s)	6, 465, 1815, 2025, 2112, 2586, 3128
FriOI	GRGCYC	4 cut(s)	116, 787, 2296, 3231
Fsp4HI	GCNGC	7 cut(s)	6, 465, 1815, 2025, 2112, 2586, 3128
FspBI	CTAG	7 cut(s)	279, 1245, 1266, 2289, 3114, 3146, 3493
FspI	TGCGCA	1 cut(s)	3126
GlaI	GCGC	6 cut(s)	617, 1875, 1969, 2216, 2702, 3126
GluI	GCNGC	7 cut(s)	6, 465, 1815, 2025, 2112, 2586, 3128
GsaI	CCCAGC	1 cut(s)	1880
GsuI	CTGGAG	2 cut(s)	264, 546
HaeII	RGCGCY	2 cut(s)	1971, 2704
HaeIII	GGCC	5 cut(s)	114, 481, 1218, 1296, 1881
HapII	CCGG	2 cut(s)	167, 2954
HgaI	GACGC	1 cut(s)	3403
HhaI	GCGC	6 cut(s)	618, 1876, 1970, 2217, 2703, 3127
Hin1I	GRCGYC	3 cut(s)	148, 341, 1968
Hin6I	GCGC	6 cut(s)	616, 1874, 1968, 2215, 2701, 3125
HinP1I	GCGC	6 cut(s)	616, 1874, 1968, 2215, 2701, 3125
HincII	GTYRAC	3 cut(s)	1000, 1957, 3073
HindII	GTYRAC	3 cut(s)	1000, 1957, 3073
HindIII	AAGCTT	3 cut(s)	1283, 2692, 3465
HpaI	GTTAAC	1 cut(s)	1957
HpaII	CCGG	2 cut(s)	167, 2954
HphI	GGTGA	6 cut(s)	569, 902, 1775, 2077, 2084, 2224
Hpy99I	CGWCG	1 cut(s)	695
HpyCH4IV	ACGT	3 cut(s)	148, 341, 1774
HpySE526I	ACGT	3 cut(s)	148, 341, 1774
Hsp92I	GRCGYC	3 cut(s)	148, 341, 1968
HspAI	GCGC	6 cut(s)	616, 1874, 1968, 2215, 2701, 3125
KasI	GGCGCC	1 cut(s)	1967
Ksp22I	TGATCA	1 cut(s)	1339
KspAI	GTTAAC	1 cut(s)	1957
LguI	GCTCTTC	1 cut(s)	310
LmnI	GCTCC	9 cut(s)	245, 505, 1069, 2212, 2473, 2684, 2698, 3234, 3373
Lsp1109I	GCAGC	4 cut(s)	476, 2036, 2098, 3139
MaeI	CTAG	7 cut(s)	279, 1245, 1266, 2289, 3114, 3146, 3493
MaeII	ACGT	3 cut(s)	148, 341, 1774
MaeIII	GTNAC	7 cut(s)	1401, 1634, 1660, 1675, 1753, 1939, 3283
MfeI	CAATTG	1 cut(s)	1617
MflI	RGATCY	3 cut(s)	418, 865, 3175
MhlI	GDGCHC	5 cut(s)	116, 250, 787, 2296, 3231
MlsI	TGGCCA	2 cut(s)	481, 1218
MluNI	TGGCCA	2 cut(s)	481, 1218
Mly113I	GGCGCC	1 cut(s)	1968
MlyI	GAGTC	4 cut(s)	1631, 2531, 2891, 3101
MmeI	TCCRAC	6 cut(s)	71, 411, 1959, 2437, 2617, 2715
Mox20I	TGGCCA	2 cut(s)	481, 1218
Mph1103I	ATGCAT	3 cut(s)	1425, 2641, 3325
MroXI	GAANNNNTTC	3 cut(s)	304, 1458, 2464
MscI	TGGCCA	2 cut(s)	481, 1218
MseI	TTAA	9 cut(s)	1077, 1356, 1476, 1578, 1793, 1956, 2508, 2564, 2786
MslI	CAYNNNNRTG	3 cut(s)	671, 2061, 3221
Msp20I	TGGCCA	2 cut(s)	481, 1218
MspA1I	CMGCKG	2 cut(s)	2111, 3130
MspI	CCGG	2 cut(s)	167, 2954
MspR9I	CCNGG	6 cut(s)	168, 525, 1410, 3233, 3253, 3297
MunI	CAATTG	1 cut(s)	1617
Mva1269I	GAATGC	1 cut(s)	30
MvaI	CCWGG	5 cut(s)	525, 1410, 3233, 3253, 3297
MvnI	CGCG	2 cut(s)	762, 996
NarI	GGCGCC	1 cut(s)	1968
NciI	CCSGG	1 cut(s)	168
NdeI	CATATG	3 cut(s)	433, 723, 1516
NlaIV	GGNNCC	6 cut(s)	113, 114, 507, 1052, 1969, 3230
NmuCI	GTSAC	3 cut(s)	1401, 1634, 1660
NsbI	TGCGCA	1 cut(s)	3126
NsiI	ATGCAT	3 cut(s)	1425, 2641, 3325
NspI	RCATGY	6 cut(s)	464, 680, 737, 2060, 2221, 3327
NspV	TTCGAA	1 cut(s)	1510
PaeI	GCATGC	2 cut(s)	2221, 3327
PagI	TCATGA	1 cut(s)	1111
PciI	ACATGT	1 cut(s)	733
PciSI	GCTCTTC	1 cut(s)	310
PctI	GAATGC	1 cut(s)	30
PdmI	GAANNNNTTC	3 cut(s)	304, 1458, 2464
PfeI	GAWTC	6 cut(s)	854, 1459, 1492, 2402, 2497, 3259
PflMI	CCANNNNNTGG	1 cut(s)	1695
PfoI	TCCNGGA	1 cut(s)	523
PkrI	GCNGC	7 cut(s)	7, 466, 1816, 2026, 2113, 2587, 3129
Ple19I	CGATCG	1 cut(s)	2031
PleI	GAGTC	4 cut(s)	1631, 2530, 2890, 3100
PluTI	GGCGCC	1 cut(s)	1971
PpsI	GAGTC	4 cut(s)	1631, 2530, 2890, 3100
PpuMI	RGGWCCY	2 cut(s)	2134, 3041
PscI	ACATGT	1 cut(s)	733
PshBI	ATTAAT	2 cut(s)	1578, 2508
PsiI	TTATAA	1 cut(s)	3245
Psp124BI	GAGCTC	1 cut(s)	2296
Psp5II	RGGWCCY	2 cut(s)	2134, 3041
Psp6I	CCWGG	5 cut(s)	523, 1408, 3231, 3251, 3295
PspFI	CCCAGC	1 cut(s)	1876
PspGI	CCWGG	5 cut(s)	523, 1408, 3231, 3251, 3295
PspN4I	GGNNCC	6 cut(s)	113, 114, 507, 1052, 1969, 3230
PspOMI	GGGCCC	1 cut(s)	112
PspPI	GGNCC	8 cut(s)	44, 112, 113, 164, 446, 1879, 2134, 3041
PspPPI	RGGWCCY	2 cut(s)	2134, 3041
PstI	CTGCAG	2 cut(s)	2026, 3142
PstNI	CAGNNNCTG	1 cut(s)	3044
PsuI	RGATCY	3 cut(s)	418, 865, 3175
PvuI	CGATCG	1 cut(s)	2031
PvuII	CAGCTG	2 cut(s)	2111, 3130
RsaI	GTAC	6 cut(s)	472, 847, 1124, 2430, 2610, 2913
RsaNI	GTAC	6 cut(s)	471, 846, 1123, 2429, 2609, 2912
RseI	CAYNNNNRTG	3 cut(s)	671, 2061, 3221
SacI	GAGCTC	1 cut(s)	2296
SapI	GCTCTTC	1 cut(s)	310
SaqAI	TTAA	9 cut(s)	1077, 1356, 1476, 1578, 1793, 1956, 2508, 2564, 2786
SatI	GCNGC	7 cut(s)	6, 465, 1815, 2025, 2112, 2586, 3128
Sau96I	GGNCC	8 cut(s)	44, 112, 113, 164, 446, 1879, 2134, 3041
SchI	GAGTC	4 cut(s)	1631, 2531, 2891, 3101
ScrFI	CCNGG	6 cut(s)	168, 525, 1410, 3233, 3253, 3297
SduI	GDGCHC	5 cut(s)	116, 250, 787, 2296, 3231
SfcI	CTRYAG	3 cut(s)	2022, 2236, 3138
SfoI	GGCGCC	1 cut(s)	1969
SfuI	TTCGAA	1 cut(s)	1510
SinI	GGWCC	5 cut(s)	44, 164, 446, 2134, 3041
SmiMI	CAYNNNNRTG	3 cut(s)	671, 2061, 3221
SmlI	CTYRAG	3 cut(s)	398, 494, 2612
SmoI	CTYRAG	3 cut(s)	398, 494, 2612
SphI	GCATGC	2 cut(s)	2221, 3327
SsiI	CCGC	7 cut(s)	5, 82, 644, 949, 1814, 2586, 3203
SspDI	GGCGCC	1 cut(s)	1967
SspI	AATATT	1 cut(s)	1551
SspMI	CTAG	7 cut(s)	279, 1245, 1266, 2289, 3114, 3146, 3493
SstI	GAGCTC	1 cut(s)	2296
StyD4I	CCNGG	6 cut(s)	166, 523, 1408, 3231, 3251, 3295
StyI	CCWWGG	3 cut(s)	745, 1971, 3479
TaiI	ACGT	3 cut(s)	151, 344, 1777
TaqI	TCGA	7 cut(s)	592, 690, 787, 1510, 2880, 2965, 3095
TatI	WGTACW	3 cut(s)	470, 845, 2911
TauI	GCSGC	3 cut(s)	8, 1817, 2588
TfiI	GAWTC	6 cut(s)	854, 1459, 1492, 2402, 2497, 3259
Tru1I	TTAA	9 cut(s)	1077, 1356, 1476, 1578, 1793, 1956, 2508, 2564, 2786
Tru9I	TTAA	9 cut(s)	1077, 1356, 1476, 1578, 1793, 1956, 2508, 2564, 2786
TscAI	CASTG	5 cut(s)	825, 1378, 1678, 2817, 3106
TseFI	GTSAC	3 cut(s)	1401, 1634, 1660
TseI	GCWGC	4 cut(s)	464, 2024, 2111, 3127
Tsp45I	GTSAC	3 cut(s)	1401, 1634, 1660
TspGWI	ACGGA	4 cut(s)	2543, 2543, 3182, 3406
TspRI	CASTG	5 cut(s)	825, 1378, 1678, 2817, 3106
Van91I	CCANNNNNTGG	1 cut(s)	1695
VpaK11BI	GGWCC	5 cut(s)	44, 164, 446, 2134, 3041
VspI	ATTAAT	2 cut(s)	1578, 2508
XagI	CCTNNNNNAGG	1 cut(s)	1358
XceI	RCATGY	6 cut(s)	464, 680, 737, 2060, 2221, 3327
XmiI	GTMKAC	1 cut(s)	121
XmnI	GAANNNNTTC	3 cut(s)	304, 1458, 2464
XspI	CTAG	7 cut(s)	279, 1245, 1266, 2289, 3114, 3146, 3493
ZraI	GACGTC	2 cut(s)	149, 342
Zsp2I	ATGCAT	3 cut(s)	1425, 2641, 3325

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.