Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0003778

Physical Location & Seq

Genomic Coordinates Reverse (-)

13640998 .. 13698886

Gene Structure 57889 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022866

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3177 bp

ATGAATTGCCGCGGCGGAGGAGGACTTGGGAGTGGAACCGCCGTCATCGTTGGCAGAGAAGTCGTCGACTTCGAATTCGGCGCTGTGATCTGCGGAGAAGTGGACCAGCCAGTCGTCGAGGTCGATTCCTCTGGTGACGGCGCTGTGATGGGCGGCCCGAGTCTGGAGGAAAGAGGTAGACTGCCTGGGTCGAGCGCCGGAGCTCGTGAGCCTGAGCTGGAGATGGCTTGGCTCGTGCGCCGGAGCCGGAGTTCGCCCCATCTTCGATACTCCTTAAAGCCAATGGCGGCTATTAGTTCTCTGGTGGGAAGCATTCCCCAAATTGGTCCGACAATATGCAGAGATGATGGTTATTCAAAAAGGCAGGCGAAGAATTCTGTGGTTTCTATTAGGGGCCCTCGTCTACCCTTATTTTCTTCTCAGACCAGACGTCTGCTTCAAGTTGGTCCGGGTAAATCAATTTTACCAAGAAAGAAGAGGAATAGTTGGGAACAATGCATCTCCATATTTGGAGAGCCTTTGACTGGAGCACCTTTTCCACGAAAGTATAACGGCATTTCTAGCTCTTTTTGCCAAAGCAGAAGCTCTTTATGGCAAAGCAGAAGTCGTCTTCGCCTCAGGACGTCCACACCAAGTGCCTTTCCTGACAGAACTTCTTTCTGTTTATCCAACCGTAAACTTGAGAAGGCTTTTGTGAAGGATCTGCATATTCCATATGCAACTGTAGGTCCAGATGAGCCACATGCAGCAAGTACATCTTGGCCAGATGGTATTCTTGAGAAACAGGAGCCAGATTTAGTATATCCTGGAGTAGGACAAACAGAGGTAGAGGGGTTTCTAAGTTCTGAACTTCCATCTCACCCAAAGTTGTATCGAGGACAACTTAAAAATGGTCTGCGCTATCTGATTTTACCAAATAAAGTTCCGCCAAACAGGTTTGAAGCACACATGGAAGTGCATGTAGGATCAATCGACGAGGAAGAGGATGAGCAAGGGATTGCACATATGATTGAACATGTTGCTTTCCTTGGAAGTAAGAAACGCGAGAAACTTCTTGGGACAGGGGCTCGATCCAATGCTTACACCGATTTCCATCATACAGTGTTTCACATCCATTCGCCACTTAGTACAAAGGATTCTGATGAAGATCTCCTTCCAAATGTGCTGGATGCCTTGAATGAGATTGCTTTTCACCCAAAATTCCTTTCTTCTCGTGTTGAGAAGGAACGGCGGGCTATACTTTCAGAATTACAGATGATGAATACAATAGATTATCGCGTTGACTGCCAGTTATTACAACATCTGCACTCTGAAAACAAGCTAAGCAAAAGGTTCCCGATTGGATTAGAGGAGCAGATTAAGAAGTGGGATGTAGATAAAGTGCGAAAATTTCATGAGCGTTGGTACTTCCCTGCAAATGCAACCTTATACATTGTTGGGGATATTGACAACATCTCAAAAACAATTTACCAAATTGAGGCTGTCTTTGGACAAACTGGCCAAGAAAATGGGTCAGCACCTACTCCTAGTGCGTTTGGTGCAATGGCTAGTTTCCTTGTCCCTAAGCTTTCAGTTGGCCTGACTGGAAATTTGCCTACTGAAAGATCGTCTAATTCCATAGATCAAACAAAACTCCTTAAAAAGGAAAAGCACACTGTTCGTCCTCCTGTGAAGCATAATTGGTCACTCCCTGGAAGTAGTATGCATTTGAAGCCTCCACAGATTTTTCAGCATGAATTGATTCAGAATTTCTCATTTAATATGTTCTGCAAGATTCCTGTGAACAGGGTTCGAACATATGGCGACTTGCGGAATGTTCTAATGAAGAGAATATTTCTTTCTGCTTTGCATTTCCGAATTAATACAAGATACAAGAGTTCAAACCCACCATTTACTTCAATTGAATTGGATCATAGTGACTCTGGAAGGGAAGGATGCACAGTCACTACTCTTACTGTAACTGCTGAACCCAAGAATTGGCAAACTGCAATAAGAGTTGCTGTTCACGAGGTTAGAAGGCTCAAAGAATTTGGTGTTACTAAGGGTGAATTGACACGTTATATAGATGCTTTATTAAAAGATAGTGAGCATCTGGCGGCTATGATAGATAATGTATCTTCGGTTGATAATTTGGATTTCATTATGGAGAGTGATGCGCTGGGCCATACAGTAATGGATCAGAGACAAGGACATGACAGTCTGGTTGCTGTTGCTGGAACTGTTACTCTTGAAGAGGTTAACTCTATTGGCGCCAAGGTGTTGGAATTTGTTTCTGATTTTGGAAAGCCTACTGCACCCCTTCCTGCAGCGATCGTTGCTTGTGTTCCAAAGAAAGTGCATGTTGATGGAAAGGGTGAAACTGAGTTCACCATTTCCCCTGATGAGATTACAGCTGCTACGAGAGCTGGATTGGAGGACCCCATTGAACCTGAGCCAGAGCTTGAAGTGCCAAAAGAATTGATATCTTCATCGCAGTTGCAGGAATTGAGGCAGGAGCGCATGCCATCCTTCATCACCTGTAGCCCAGAAACAAATATGACAAAAATTTATGACAAGGAAACAGGGATCACTAGAGCTCGTCTTTCAAATGGAATTGCTGTAAATTACAAGATTTCAAAAAGTGAGGCTCGGGGAGGTGTGATGAGGCTTATAGTTGGTGGTGGACGGGCAACTGAAAGTCCTGAATCAAAAGGTTCTGTTGTTGTGGGTGTACGAACTCTTAGCGAGGGAGGTCGTGTTGGAAACTTTTCAAGGGAGCAGGTAGAACTGTTCTGTGTGAATCACTTGATTAATTGCTCATTGGAGTCCACGGAGGAATTTATTTCTATGGAATTCCGTTTCACTTTAAGAGATAATGGGATGCGTGCCGCTTTCCAATTGCTTCACATGGTACTTGAGCATAGTGTCTGGCTGGATGATGCATTTGATAGAGCAAGACAATTATATTTGTCATATTATCGCTCCATCCCTAAAAGCTTGGAGCGATCAACTGCTCACAAACTCATGTTGGCAATGTTGGACGGGGATGAGCGTTTTGCAGAACCTACACCAACATCATTGCAAAATTTAACTTTGCAATCTGTGAAGGATGCAGTGATGAACCAATTTGTTGGCAATAACATGGAGGTGTGCATATCCTACCTGCTGATTTGTATTTTATTATTATTTTTTCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0007275 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M16 Peptidase_M16_C

Protein Analysis

1059

Amino Acids

117.86

Weight (kDa)

7.04

Isoelectric Point (pI)

42.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	313 - 437	1.1e-20	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	456 - 691	1.5e-12	Peptidase M16 inactive domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003778)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42390 AT5G42390
fragaria_vesca	FvH4_1g30291 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800
malus_domestica	MD00G1159400.v1.1 MD00G1204700.v1.1
prunus_persica	Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1
pyrus_communis	pycom290g00010
rosa_chinensis	RchiOBHm_Chr3g0491051
rosa_laevigata	RLG00000022866
rosa_multiflora	Rmu_co8044182.1_g000001 Rmu_ssc0000386.1_g000043
rosa_roxburghii	Rroxscaffold_5G00351210 Rroxscaffold_6G00393270
rosa_rugosa	Rorug03G0245500 Rorug04G0069600 Rorug04G0069700 Rorug04G0070700 Rorug04G0070800
rosa_samantha	Rh3AG295700 Rh3BG331900 Rh3CG329000 Rh3DG329500
rosa_wichuraiana	Rw3G026110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	110, 2196
AatII	GACGTC	2 cut(s)	433, 626
Acc36I	ACCTGC	2 cut(s)	2749, 3150
AccB1I	GGYRCC	1 cut(s)	2249
AccB7I	CCANNNNNTGG	1 cut(s)	1977
AccI	GTMKAC	3 cut(s)	66, 178, 403
AccII	CGCG	3 cut(s)	12, 1044, 1278
AclWI	GGATC	6 cut(s)	708, 973, 1065, 1917, 2184, 2573
AcoI	YGGCCR	2 cut(s)	761, 1498
AcyI	GRCGYC	3 cut(s)	430, 623, 2250
AdeI	CACNNNGTG	1 cut(s)	635
AfaI	GTAC	5 cut(s)	754, 1129, 1406, 2712, 2892
AfiI	CCNNNNNNNGG	7 cut(s)	163, 323, 524, 812, 1477, 1642, 1977
AflIII	ACRYGT	2 cut(s)	1015, 2054
AhdI	GACNNNNNGTC	1 cut(s)	429
AjnI	CCWGG	3 cut(s)	184, 805, 1690
AjuI	GAANNNNNNNTTGG	2 cut(s)	1189, 1221
AloI	GAACNNNNNNTCC	2 cut(s)	235, 267
Alw21I	GWGCWC	3 cut(s)	205, 532, 2578
Alw26I	GTCTC	1 cut(s)	2176
AlwI	GGATC	6 cut(s)	708, 973, 1065, 1917, 2184, 2573
Ama87I	CYCGRG	2 cut(s)	157, 2628
AoxI	GGCC	6 cut(s)	154, 394, 761, 1498, 1576, 2161
ApaI	GGGCCC	1 cut(s)	398
ApeKI	GCWGC	3 cut(s)	746, 2306, 2393
ArsI	GACNNNNNNTTYG	6 cut(s)	59, 91, 589, 621, 1573, 1605
AseI	ATTAAT	2 cut(s)	1860, 2790
Asp700I	GAANNNNTTC	2 cut(s)	1740, 2746
AspLEI	GCGC	8 cut(s)	83, 143, 197, 240, 900, 2158, 2252, 2499
AspS9I	GGNCC	9 cut(s)	103, 155, 326, 394, 395, 446, 728, 2161, 2416
AsuC2I	CCSGG	1 cut(s)	450
AsuHPI	GGTGA	7 cut(s)	146, 851, 1184, 2057, 2359, 2366, 2506
AsuII	TTCGAA	2 cut(s)	72, 1792
AvaI	CYCGRG	2 cut(s)	157, 2628
AvaII	GGWCC	5 cut(s)	103, 326, 446, 728, 2416
AxyI	CCTNAGG	1 cut(s)	617
BaeGI	GKGCMC	1 cut(s)	398
BaeI	ACNNNNGTAYC	2 cut(s)	1861, 1894
BalI	TGGCCA	2 cut(s)	763, 1500
BanI	GGYRCC	1 cut(s)	2249
BanII	GRGCYC	4 cut(s)	205, 398, 1069, 2578
BauI	CACGAG	4 cut(s)	204, 233, 1212, 2006
BbsI	GAAGAC	1 cut(s)	602
Bbv12I	GWGCWC	3 cut(s)	205, 532, 2578
BbvI	GCAGC	3 cut(s)	758, 2318, 2380
BceAI	ACGGC	4 cut(s)	26, 154, 568, 1244
BcgI	CGANNNNNNTGC	4 cut(s)	43, 77, 1640, 1674
BciT130I	CCWGG	3 cut(s)	186, 807, 1692
BcnI	CCSGG	1 cut(s)	450
BcoDI	GTCTC	1 cut(s)	2176
BfaI	CTAG	4 cut(s)	561, 1527, 1548, 2571
BfmI	CTRYAG	3 cut(s)	723, 2304, 2518
BfoI	RGCGCY	4 cut(s)	84, 144, 198, 2253
BfuAI	ACCTGC	2 cut(s)	2749, 3150
BglII	AGATCT	1 cut(s)	1147
BisI	GCNGC	9 cut(s)	10, 13, 154, 288, 747, 2097, 2307, 2394, 2868
BlpI	GCTNAGC	1 cut(s)	1322
BlsI	GCNGC	9 cut(s)	11, 14, 155, 289, 748, 2098, 2308, 2395, 2869
Bme1390I	CCNGG	4 cut(s)	186, 450, 807, 1692
Bme18I	GGWCC	5 cut(s)	103, 326, 446, 728, 2416
BmeRI	GACNNNNNGTC	1 cut(s)	429
BmeT110I	CYCGRG	2 cut(s)	157, 2628
BmgT120I	GGNCC	9 cut(s)	103, 155, 326, 394, 395, 446, 728, 2161, 2416
BmiI	GGNNCC	8 cut(s)	37, 245, 395, 396, 789, 1334, 2251, 2418
BmrFI	CCNGG	4 cut(s)	186, 450, 807, 1692
BmsI	GCATC	9 cut(s)	507, 1159, 1925, 2056, 2098, 2143, 2850, 2908, 3079
BpiI	GAAGAC	1 cut(s)	602
BplI	GAGNNNNNCTC	2 cut(s)	2225, 2257
BpmI	CTGGAG	4 cut(s)	185, 239, 546, 828
Bpu10I	CCTNAGC	3 cut(s)	213, 1563, 2430
Bpu1102I	GCTNAGC	1 cut(s)	1322
Bpu14I	TTCGAA	2 cut(s)	72, 1792
BpuEI	CTTGAG	3 cut(s)	701, 797, 2915
BpuMI	CCSGG	1 cut(s)	450
BsaBI	GATNNNNATC	2 cut(s)	1092, 1146
BsaHI	GRCGYC	3 cut(s)	430, 623, 2250
BsaJI	CCNNGG	6 cut(s)	10, 185, 1027, 1690, 2253, 2808
BsaXI	ACNNNNNCTCC	4 cut(s)	235, 265, 2721, 2751
Bsc4I	CCNNNNNNNGG	7 cut(s)	163, 323, 524, 812, 1477, 1642, 1977
Bse1I	ACTGG	5 cut(s)	110, 529, 1288, 1501, 1588
Bse21I	CCTNAGG	1 cut(s)	617
Bse3DI	GCAATG	3 cut(s)	1548, 3018, 3056
Bse8I	GATNNNNATC	2 cut(s)	1092, 1146
BseBI	CCWGG	3 cut(s)	186, 807, 1692
BseDI	CCNNGG	6 cut(s)	10, 185, 1027, 1690, 2253, 2808
BseJI	GATNNNNATC	2 cut(s)	1092, 1146
BseLI	CCNNNNNNNGG	7 cut(s)	163, 323, 524, 812, 1477, 1642, 1977
BseMI	GCAATG	3 cut(s)	1548, 3018, 3056
BseMII	CTCAG	5 cut(s)	204, 434, 631, 2352, 2421
BseNI	ACTGG	5 cut(s)	110, 529, 1288, 1501, 1588
BseRI	GAGGAG	2 cut(s)	33, 1364
BseSI	GKGCMC	1 cut(s)	398
BseXI	GCAGC	3 cut(s)	758, 2318, 2380
BseYI	CCCAGC	1 cut(s)	2158
BsgI	GTGCAG	2 cut(s)	1289, 2277
Bsh1236I	CGCG	3 cut(s)	12, 1044, 1278
Bsh1285I	CGRYCG	1 cut(s)	2313
BshFI	GGCC	6 cut(s)	156, 396, 763, 1500, 1578, 2163
BshNI	GGYRCC	1 cut(s)	2249
BsiEI	CGRYCG	1 cut(s)	2313
BsiHKAI	GWGCWC	3 cut(s)	205, 532, 2578
BsiHKCI	CYCGRG	2 cut(s)	157, 2628
BsiSI	CCGG	4 cut(s)	198, 241, 247, 449
BslFI	GGGAC	2 cut(s)	1072, 1544
BslI	CCNNNNNNNGG	7 cut(s)	163, 323, 524, 812, 1477, 1642, 1977
BsmAI	GTCTC	1 cut(s)	2176
BsmFI	GGGAC	2 cut(s)	1072, 1544
BsmI	GAATGC	1 cut(s)	312
BsnI	GGCC	6 cut(s)	156, 396, 763, 1500, 1578, 2163
BsoBI	CYCGRG	2 cut(s)	157, 2628
Bsp119I	TTCGAA	2 cut(s)	72, 1792
Bsp120I	GGGCCC	1 cut(s)	394
Bsp1286I	GDGCHC	5 cut(s)	205, 398, 532, 1069, 2578
Bsp1720I	GCTNAGC	1 cut(s)	1322
BspANI	GGCC	6 cut(s)	156, 396, 763, 1500, 1578, 2163
BspCNI	CTCAG	5 cut(s)	205, 433, 630, 2353, 2422
BspFNI	CGCG	3 cut(s)	12, 1044, 1278
BspHI	TCATGA	1 cut(s)	1393
BspLI	GGNNCC	8 cut(s)	37, 245, 395, 396, 789, 1334, 2251, 2418
BspMAI	CTGCAG	1 cut(s)	2308
BspMI	ACCTGC	2 cut(s)	2749, 3150
BspPI	GGATC	6 cut(s)	708, 973, 1065, 1917, 2184, 2573
BspT104I	TTCGAA	2 cut(s)	72, 1792
BspT107I	GGYRCC	1 cut(s)	2249
BsrDI	GCAATG	3 cut(s)	1548, 3018, 3056
BsrI	ACTGG	5 cut(s)	110, 529, 1288, 1501, 1588
BssECI	CCNNGG	6 cut(s)	10, 185, 1027, 1690, 2253, 2808
BssNI	GRCGYC	3 cut(s)	430, 623, 2250
BssSI	CACGAG	4 cut(s)	204, 233, 1212, 2006
BssT1I	CCWWGG	2 cut(s)	1027, 2253
Bst2BI	CACGAG	4 cut(s)	204, 233, 1212, 2006
Bst2UI	CCWGG	3 cut(s)	186, 807, 1692
Bst6I	CTCTTC	4 cut(s)	470, 975, 1820, 2226
BstACI	GRCGYC	3 cut(s)	430, 623, 2250
BstBI	TTCGAA	2 cut(s)	72, 1792
BstC8I	GCNNGC	4 cut(s)	366, 1233, 2501, 2865
BstDSI	CCRYGG	2 cut(s)	10, 2808
BstENI	CCTNNNNNAGG	2 cut(s)	810, 1640
BstFNI	CGCG	3 cut(s)	12, 1044, 1278
BstH2I	RGCGCY	4 cut(s)	84, 144, 198, 2253
BstHHI	GCGC	8 cut(s)	83, 143, 197, 240, 900, 2158, 2252, 2499
BstMAI	GTCTC	1 cut(s)	2176
BstMCI	CGRYCG	1 cut(s)	2313
BstMWI	GCNNNNNNNGC	8 cut(s)	561, 570, 1284, 1538, 1711, 2315, 2402, 2446
BstNI	CCWGG	3 cut(s)	186, 807, 1692
BstNSI	RCATGY	5 cut(s)	746, 962, 1019, 2342, 2503
BstSCI	CCNGG	4 cut(s)	184, 448, 805, 1690
BstSFI	CTRYAG	3 cut(s)	723, 2304, 2518
BstSLI	GKGCMC	1 cut(s)	398
BstUI	CGCG	3 cut(s)	12, 1044, 1278
BstV1I	GCAGC	3 cut(s)	758, 2318, 2380
BstV2I	GAAGAC	1 cut(s)	602
BstX2I	RGATCY	2 cut(s)	700, 1147
BstXI	CCANNNNNNTGG	3 cut(s)	1508, 2260, 3110
BstYI	RGATCY	2 cut(s)	700, 1147
Bsu36I	CCTNAGG	1 cut(s)	617
BsuRI	GGCC	6 cut(s)	156, 396, 763, 1500, 1578, 2163
BtgI	CCRYGG	2 cut(s)	10, 2808
BtgZI	GCGATG	1 cut(s)	2454
BtsI	GCAGTG	1 cut(s)	3099
BtsIMutI	CAGTG	3 cut(s)	1107, 1653, 3099
BveI	ACCTGC	2 cut(s)	2749, 3150
Cac8I	GCNNGC	4 cut(s)	366, 1233, 2501, 2865
CciI	TCATGA	1 cut(s)	1393
CfoI	GCGC	8 cut(s)	83, 143, 197, 240, 900, 2158, 2252, 2499
Cfr13I	GGNCC	9 cut(s)	103, 155, 326, 394, 395, 446, 728, 2161, 2416
Cfr42I	CCGCGG	1 cut(s)	13
Csp6I	GTAC	5 cut(s)	753, 1128, 1405, 2711, 2891
CviQI	GTAC	5 cut(s)	753, 1128, 1405, 2711, 2891
DinI	GGCGCC	1 cut(s)	2251
DraIII	CACNNNGTG	1 cut(s)	635
DrdI	GACNNNNNNGTC	2 cut(s)	110, 2196
DriI	GACNNNNNGTC	1 cut(s)	429
DseDI	GACNNNNNNGTC	2 cut(s)	110, 2196
EaeI	YGGCCR	2 cut(s)	761, 1498
Eam1104I	CTCTTC	4 cut(s)	470, 975, 1820, 2226
Eam1105I	GACNNNNNGTC	1 cut(s)	429
EarI	CTCTTC	4 cut(s)	470, 975, 1820, 2226
EciI	GGCGGA	2 cut(s)	30, 915
Ecl136II	GAGCTC	2 cut(s)	203, 2576
Eco130I	CCWWGG	2 cut(s)	1027, 2253
Eco24I	GRGCYC	4 cut(s)	205, 398, 1069, 2578
Eco32I	GATATC	1 cut(s)	2463
Eco47I	GGWCC	5 cut(s)	103, 326, 446, 728, 2416
Eco53kI	GAGCTC	2 cut(s)	203, 2576
Eco81I	CCTNAGG	1 cut(s)	617
Eco88I	CYCGRG	2 cut(s)	157, 2628
EcoICRI	GAGCTC	2 cut(s)	203, 2576
EcoNI	CCTNNNNNAGG	2 cut(s)	810, 1640
EcoO109I	RGGNCCY	3 cut(s)	394, 395, 2416
EcoRI	GAATTC	3 cut(s)	74, 373, 2831
EcoRII	CCWGG	3 cut(s)	184, 805, 1690
EcoRV	GATATC	1 cut(s)	2463
EcoT14I	CCWWGG	2 cut(s)	1027, 2253
EcoT22I	ATGCAT	3 cut(s)	500, 1707, 2923
EcoT38I	GRGCYC	4 cut(s)	205, 398, 1069, 2578
EgeI	GGCGCC	1 cut(s)	2251
EheI	GGCGCC	1 cut(s)	2251
ErhI	CCWWGG	2 cut(s)	1027, 2253
FalI	AAGNNNNNCTT	4 cut(s)	742, 774, 1137, 1169
FaqI	GGGAC	2 cut(s)	1072, 1544
FauI	CCCGC	1 cut(s)	1224
FauNDI	CATATG	3 cut(s)	715, 1005, 1798
FblI	GTMKAC	3 cut(s)	66, 178, 403
Fnu4HI	GCNGC	9 cut(s)	10, 13, 154, 288, 747, 2097, 2307, 2394, 2868
FriOI	GRGCYC	4 cut(s)	205, 398, 1069, 2578
Fsp4HI	GCNGC	9 cut(s)	10, 13, 154, 288, 747, 2097, 2307, 2394, 2868
FspBI	CTAG	4 cut(s)	561, 1527, 1548, 2571
GlaI	GCGC	8 cut(s)	82, 142, 196, 239, 899, 2157, 2251, 2498
GluI	GCNGC	9 cut(s)	10, 13, 154, 288, 747, 2097, 2307, 2394, 2868
GsaI	CCCAGC	1 cut(s)	2162
GsuI	CTGGAG	4 cut(s)	185, 239, 546, 828
HaeII	RGCGCY	4 cut(s)	84, 144, 198, 2253
HaeIII	GGCC	6 cut(s)	156, 396, 763, 1500, 1578, 2163
HapII	CCGG	4 cut(s)	198, 241, 247, 449
HhaI	GCGC	8 cut(s)	83, 143, 197, 240, 900, 2158, 2252, 2499
Hin1I	GRCGYC	3 cut(s)	430, 623, 2250
Hin6I	GCGC	8 cut(s)	81, 141, 195, 238, 898, 2156, 2250, 2497
HinP1I	GCGC	8 cut(s)	81, 141, 195, 238, 898, 2156, 2250, 2497
HincII	GTYRAC	3 cut(s)	67, 1282, 2239
HindII	GTYRAC	3 cut(s)	67, 1282, 2239
HindIII	AAGCTT	2 cut(s)	1565, 2974
HinfI	GANTC	9 cut(s)	125, 160, 1136, 1741, 1774, 1919, 2684, 2779, 2804
HpaI	GTTAAC	1 cut(s)	2239
HpaII	CCGG	4 cut(s)	198, 241, 247, 449
HphI	GGTGA	7 cut(s)	146, 851, 1184, 2057, 2359, 2366, 2506
Hpy99I	CGWCG	3 cut(s)	68, 119, 977
HpyCH4IV	ACGT	3 cut(s)	430, 623, 2056
HpyF10VI	GCNNNNNNNGC	8 cut(s)	561, 570, 1284, 1538, 1711, 2315, 2402, 2446
HpySE526I	ACGT	3 cut(s)	430, 623, 2056
Hsp92I	GRCGYC	3 cut(s)	430, 623, 2250
HspAI	GCGC	8 cut(s)	81, 141, 195, 238, 898, 2156, 2250, 2497
KasI	GGCGCC	1 cut(s)	2249
KspAI	GTTAAC	1 cut(s)	2239
KspI	CCGCGG	1 cut(s)	13
LmnI	GCTCC	9 cut(s)	200, 243, 527, 787, 1351, 2494, 2755, 2966, 2980
Lsp1109I	GCAGC	3 cut(s)	758, 2318, 2380
LweI	GCATC	9 cut(s)	507, 1159, 1925, 2056, 2098, 2143, 2850, 2908, 3079
MaeI	CTAG	4 cut(s)	561, 1527, 1548, 2571
MaeII	ACGT	3 cut(s)	430, 623, 2056
MaeIII	GTNAC	7 cut(s)	134, 1683, 1916, 1942, 1957, 2035, 2221
MfeI	CAATTG	2 cut(s)	1899, 2876
MflI	RGATCY	2 cut(s)	700, 1147
MhlI	GDGCHC	5 cut(s)	205, 398, 532, 1069, 2578
MlsI	TGGCCA	2 cut(s)	763, 1500
MluNI	TGGCCA	2 cut(s)	763, 1500
Mly113I	GGCGCC	1 cut(s)	2250
MlyI	GAGTC	3 cut(s)	169, 1913, 2813
MmeI	TCCRAC	5 cut(s)	353, 693, 2241, 2719, 2997
Mox20I	TGGCCA	2 cut(s)	763, 1500
Mph1103I	ATGCAT	3 cut(s)	500, 1707, 2923
MroXI	GAANNNNTTC	2 cut(s)	1740, 2746
MscI	TGGCCA	2 cut(s)	763, 1500
MslI	CAYNNNNRTG	3 cut(s)	953, 2343, 3125
Msp20I	TGGCCA	2 cut(s)	763, 1500
MspA1I	CMGCKG	2 cut(s)	12, 2393
MspI	CCGG	4 cut(s)	198, 241, 247, 449
MspR9I	CCNGG	4 cut(s)	186, 450, 807, 1692
MunI	CAATTG	2 cut(s)	1899, 2876
Mva1269I	GAATGC	1 cut(s)	312
MvaI	CCWGG	3 cut(s)	186, 807, 1692
MvnI	CGCG	3 cut(s)	12, 1044, 1278
MwoI	GCNNNNNNNGC	8 cut(s)	561, 570, 1284, 1538, 1711, 2315, 2402, 2446
NarI	GGCGCC	1 cut(s)	2250
NciI	CCSGG	1 cut(s)	450
NdeI	CATATG	3 cut(s)	715, 1005, 1798
NlaIV	GGNNCC	8 cut(s)	37, 245, 395, 396, 789, 1334, 2251, 2418
NmuCI	GTSAC	4 cut(s)	134, 1683, 1916, 1942
NsiI	ATGCAT	3 cut(s)	500, 1707, 2923
NspI	RCATGY	5 cut(s)	746, 962, 1019, 2342, 2503
NspV	TTCGAA	2 cut(s)	72, 1792
PaeI	GCATGC	1 cut(s)	2503
PagI	TCATGA	1 cut(s)	1393
PciI	ACATGT	1 cut(s)	1015
PcsI	WCGNNNNNNNCGW	2 cut(s)	69, 120
PctI	GAATGC	1 cut(s)	312
PdmI	GAANNNNTTC	2 cut(s)	1740, 2746
PfeI	GAWTC	6 cut(s)	125, 1136, 1741, 1774, 2684, 2779
PflMI	CCANNNNNTGG	1 cut(s)	1977
PfoI	TCCNGGA	1 cut(s)	805
PkrI	GCNGC	9 cut(s)	11, 14, 155, 289, 748, 2098, 2308, 2395, 2869
Ple19I	CGATCG	1 cut(s)	2313
PleI	GAGTC	3 cut(s)	168, 1913, 2812
PluTI	GGCGCC	1 cut(s)	2253
PpsI	GAGTC	3 cut(s)	168, 1913, 2812
PpuMI	RGGWCCY	1 cut(s)	2416
PscI	ACATGT	1 cut(s)	1015
PshBI	ATTAAT	2 cut(s)	1860, 2790
Psp124BI	GAGCTC	2 cut(s)	205, 2578
Psp5II	RGGWCCY	1 cut(s)	2416
Psp6I	CCWGG	3 cut(s)	184, 805, 1690
PspFI	CCCAGC	1 cut(s)	2158
PspGI	CCWGG	3 cut(s)	184, 805, 1690
PspN4I	GGNNCC	8 cut(s)	37, 245, 395, 396, 789, 1334, 2251, 2418
PspOMI	GGGCCC	1 cut(s)	394
PspPI	GGNCC	9 cut(s)	103, 155, 326, 394, 395, 446, 728, 2161, 2416
PspPPI	RGGWCCY	1 cut(s)	2416
PstI	CTGCAG	1 cut(s)	2308
PsuI	RGATCY	2 cut(s)	700, 1147
PvuI	CGATCG	1 cut(s)	2313
PvuII	CAGCTG	1 cut(s)	2393
RsaI	GTAC	5 cut(s)	754, 1129, 1406, 2712, 2892
RsaNI	GTAC	5 cut(s)	753, 1128, 1405, 2711, 2891
RseI	CAYNNNNRTG	3 cut(s)	953, 2343, 3125
SacI	GAGCTC	2 cut(s)	205, 2578
SacII	CCGCGG	1 cut(s)	13
SalI	GTCGAC	1 cut(s)	65
SatI	GCNGC	9 cut(s)	10, 13, 154, 288, 747, 2097, 2307, 2394, 2868
Sau96I	GGNCC	9 cut(s)	103, 155, 326, 394, 395, 446, 728, 2161, 2416
SchI	GAGTC	3 cut(s)	169, 1913, 2813
ScrFI	CCNGG	4 cut(s)	186, 450, 807, 1692
SduI	GDGCHC	5 cut(s)	205, 398, 532, 1069, 2578
SfaNI	GCATC	9 cut(s)	507, 1159, 1925, 2056, 2098, 2143, 2850, 2908, 3079
SfcI	CTRYAG	3 cut(s)	723, 2304, 2518
SfoI	GGCGCC	1 cut(s)	2251
Sfr303I	CCGCGG	1 cut(s)	13
SfuI	TTCGAA	2 cut(s)	72, 1792
SgrBI	CCGCGG	1 cut(s)	13
SinI	GGWCC	5 cut(s)	103, 326, 446, 728, 2416
SmiMI	CAYNNNNRTG	3 cut(s)	953, 2343, 3125
SmlI	CTYRAG	3 cut(s)	680, 776, 2894
SmoI	CTYRAG	3 cut(s)	680, 776, 2894
SphI	GCATGC	1 cut(s)	2503
SspDI	GGCGCC	1 cut(s)	2249
SspI	AATATT	1 cut(s)	1833
SspMI	CTAG	4 cut(s)	561, 1527, 1548, 2571
SstI	GAGCTC	2 cut(s)	205, 2578
StyD4I	CCNGG	4 cut(s)	184, 448, 805, 1690
StyI	CCWWGG	2 cut(s)	1027, 2253
TaiI	ACGT	3 cut(s)	433, 626, 2059
TatI	WGTACW	2 cut(s)	752, 1127
TauI	GCSGC	6 cut(s)	12, 15, 156, 290, 2099, 2870
TfiI	GAWTC	6 cut(s)	125, 1136, 1741, 1774, 2684, 2779
TscAI	CASTG	3 cut(s)	1107, 1660, 3099
TseFI	GTSAC	4 cut(s)	134, 1683, 1916, 1942
TseI	GCWGC	3 cut(s)	746, 2306, 2393
Tsp45I	GTSAC	4 cut(s)	134, 1683, 1916, 1942
TspGWI	ACGGA	2 cut(s)	2825, 2825
TspRI	CASTG	3 cut(s)	1107, 1660, 3099
Van91I	CCANNNNNTGG	1 cut(s)	1977
VpaK11BI	GGWCC	5 cut(s)	103, 326, 446, 728, 2416
VspI	ATTAAT	2 cut(s)	1860, 2790
XagI	CCTNNNNNAGG	2 cut(s)	810, 1640
XceI	RCATGY	5 cut(s)	746, 962, 1019, 2342, 2503
XmiI	GTMKAC	3 cut(s)	66, 178, 403
XmnI	GAANNNNTTC	2 cut(s)	1740, 2746
XspI	CTAG	4 cut(s)	561, 1527, 1548, 2571
ZraI	GACGTC	2 cut(s)	431, 624
Zsp2I	ATGCAT	3 cut(s)	500, 1707, 2923

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.