Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0003778

Physical Location & Seq

Genomic Coordinates Forward (+)

49604545 .. 49615878

Gene Structure 11334 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1204700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3795 bp

ATGGCGGCCATAAGCTCTCTTGTTGTAAACGTCCCCCAAATCCGTCCGTCCCGGAGAATAAGCGGAGACGATGACACTTATTTGAATAAGCGGCTGAATTCGGTGGTCTGTATTCGCCGTGCTCGTGGCCCTCTCACCCCTTTACATATCAATCGTCTGTCTTTACTTTCTCCAACCAGGCGTCTACTTGAAGCTGCTGCAGGTAGATCAGGTGTACATAGAAAGAAGATTAATGCTTGGGAACAATGTGTCTCCATGCTTGGAGAGCCAGTGACTGAAGCACTTTTTCCAGGAAAGTATAACTGCATTACTAGCTCTTTTTGCCCAAACAACAGTCGTCGTCGCCGTAGGACATCTACACCGAGTGTCTTTAGTGACAGAACTTCTTTTTGTTTATCCAAGCCTAAACTTGGCAATGTTGCTGGCAAACATGCTCATGTTTCATGCGCAACCGTGGGCCCAGATGAGCCACACGCGGCAAGTACAGCCTGGCAAGATGGTATTCTGGAGAAACAGGAGCTGGATTTATTATATCCTGGAGTAGAAAAAACAGAGTTGGAGGGGTTTTTAAGTTCTGAAATTCCATCTCATCCAAAGTTGTATCGAGGGCAACTAAAAAATGGGCTGCGCTATCTGATTTTACCAAATAAAGTCCCGCCAAACAGGTTTGAAGCACACATGGAAGTTCATGCAGGATCAATCGATGAGGAAGACGATGAGCAAGGAATTGCACATATGATTGAACATGTTGCTTTCCTTGGAAGTAAGAAACGTGAGAAACTTCTTGGGACAGGGGCTCGATCGAATGCTTACACTGACTTCCATCATACAGTGTTTCACATCCATTCGCCCATTAGTTCAAAGGATTCTGATGGTGATCTGCTTCCGTATGTACTGGATGCCTTGAATGAGATTGCTTTTCACCCAAAATTCCTTTCTTCTCGTGTTGAAAAAGAACGGCGTGCGATTCTGTCAGAATTACAGATGATGAATACTATAGATTATCGCGTTGACTGCCAGTTATTACAACATCTGCATTCTGAAAACAAGCTTAGCAGAAGGTTCCCGATTGGGTTAGAAGAGCAGATTAAGAAGTGGGATGTAGATAAAATAAGGAAATTCCATGAGCGTTGGTACTTCCCTGCAAATGCAACCTTATACATTGTTGGGGACATTGATAACATCTCAAAAACGATTTACCAGATTGAAGCTGTCTTTGGACAAACTGGCCTAGAAAATGAGACAGTTCCTGCACCTGCTCCTAGTGCTTTTGGTGCAATGGCTAGTTTCCTTGTGCCCAAGCTTTCAGTTGGGCTGACTGGAAGTTCTAATTCCGTTGATCAAGCTAAAATCTTTAAAAAGGAAAGGCACACAGTTCGTCCTCCTGTGAAACATAATTGGTCACTTCCTGGAAGTAATATGGATTTGAAGCCTCCACAGATTTTTCAGCATGAATTGATTCAAAATTTTTCATTTAATATGTTCTGCAAGGTTCCTGTGAGCAGGGTTCAAACATATGGTGACTTGCGAAATGTTCTAATGAAGAGAATATTTCTGTCTGCTTTGCATTTCCGAATTAATACAAGATACAAGAGTTCAAATCCACCATTTACTTCAATTGAATTGGATCATAGTGACTCTGGAAGGGAAGGATGTACTGTCACTACTCTTACGGTAACTGCCGAACCCAAGAATTGGCAAAGTGCAATTAGAGTTGCTGTACAAGAGGTTAGAAGGCTCAAGGAATTTGGTGTTACTAAGGGTGAATTAACTCGCTATATGGATGCCTTATTAAAAGATAGTGAACATCTGGCCGCTATGATAGATAATGTGTCTTCTGTTGATAATTTGGATTTTATTATGGAGAGTGATGCGCTGGGGCATACAGTAATGGATCAGAGGCAAGGACACGAGAGTTTGGTTGCTGTTGCTGGAACAGTTACTCTTGAAGAGGTCAACTCTATTGGCGCCAAGGTGTTGGAATTTGTTTCTAATTTTGGAAATCCTACTGCACCTCTTCCTGCAGCGATTGTTGCTTGTGTTCCAAAGAGAGTGCATGTAGAGGGAATAGGTGAAACTGAGTTCACCATTTCCTCTAATGAGATTATAGCTGCTACAAAAGCTGGATTGGAGGAACCCATCGAACCTGAGCCAGAGCTTGAGGTGCCAAAGGAATTGATATCTTCGTTGCAGTTACAGGAATTAAGGCAGCAGTGCATGCCATCTTTCATTCCCTTTAGTCCAGAAATAAAAGTGACTAAAGTTTATGACAAAGAAACGGGGATCACTAAATGTCGTCTGTCAAATGGAATTTCTGTAAATTACAAGATTTCAAAAAGTGAGGCAAGGGGAGGTGTAATGCGGCTTATAGTTGGCGGTGGACGGGCAGCTGAAAGTTCTGATTCCAAAGGTTCTGTTGTTGTGGGTGTAAGAACTCTTAGTGAGGGAGGACGTGTTGGCAACTTCTCAAGGGAGCAGGTAGAACTGTTCTGTGTGAATCACCTGATTAACTGCTCATTGGAGTCCACCGAGGAATTTATTTCTATGGAATTCCGTTTTACTTTAAGAGATAATGGAATGCGTGCGGCTTTCCAGTTGCTTCATATGGTACTCGAGCATAGTGTCTGGCTGGATGATGCATTTGATCGAGCAAGGCAATTGTATTTGTCATATTATCGGTCCATTCCCAAAAGCTTGGAGCGCTCAACTGCTCACAAACTCATGTTAGCAATGATGGACGGAGACGAGCGGTTTGTAGAGCCTACACCAAAGTCATTGCAAAATTTGACATTGCAATCTGTAAAGGATGCAGTGATGAATCAATTTGTAGGCAGTAACATGGAGGTAAGTATTGTTGGGGACTTCTCAGAGGAGGATGTTGAGTCTTGTATTCTTGATTACTTGGGAACCGTAAGATCGAAAGGAAATTACGAGATGGAGAATGCTTATAATCCTATCGTATTCCGATCATCTCCTTCTGATTTGCAGTCTCAGCAAGTATTCTTGAAAGATACGGATGAGAGAGCATGTGCATATATTGCAGGCCCTGCACCAAACCGATGGGGTTTTACAGTCGATGGCAAAGATCTGTTTGAGTCAGTTAGTCATATTTCAACTCACGATGATGCAGAACTGAAATCGGAGGAGCTGCTTATGGTGGATGAGGATACTCAGAAGGATGTGCAAAGAAAACTCCGTGGTCATCCCCTGTTCTTTGGAATCACAATGGGGCTCCTGGCTGAGATTATAAATTCTAGGCTTTTTACGACTGTCAGAGACTCTCTTGGATTGACATATGATGTATCATTCGAGTTGAACCTTTTTGATAGGCTGAATCTTGGATGGTATGTCGTATCTGTAACATCAACCCCTGCGAAGGTTCACAAAGCTGTGGATGCATGCAAGAATGTCCTTAGAGGTTTACATGGCAACAAGATTTCCCAAAGGGAGTTGGACAGGGCAAAGCGGACCCTTCTGATGAGGCATGAAGCTGAGATCAAGTCAAATGCGTATTGGCTTGGACTGTTAGCTCATTTGCAGGCTTCTTCTGTTCCAAGGAAGGACATATCGTGCATCAAAGATCTCACTAATTTATATGAGGTTGCTTCTATTGAGGACATATACCTTGCATATGATCAATTGAAAGTAGGTGATGATTCTTTGTACTCATGCATCGGAGTTGCTGGAGCTCAAGCAGTAGATGAAATAACTGAAGTCGAAGAATCAGATGATGGCTTTCCAGGAATCTTCCCTGTTGGGCGTGGTTTATCAACCATGACACGGCCTACAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0007275 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M16 Peptidase_M16_C

Protein Analysis

1265

Amino Acids

141.23

Weight (kDa)

6.17

Isoelectric Point (pI)

44.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	223 - 347	5.2e-21	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	366 - 597	4.3e-12	Peptidase M16 inactive domain
Peptidase_M16_C	PF05193	928 - 1158	8.4e-23	Peptidase M16 inactive domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003778)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42390 AT5G42390
fragaria_vesca	FvH4_1g30291 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800
malus_domestica	MD00G1159400.v1.1 MD00G1204700.v1.1
prunus_persica	Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1
pyrus_communis	pycom290g00010
rosa_chinensis	RchiOBHm_Chr3g0491051
rosa_laevigata	RLG00000022866
rosa_multiflora	Rmu_co8044182.1_g000001 Rmu_ssc0000386.1_g000043
rosa_roxburghii	Rroxscaffold_5G00351210 Rroxscaffold_6G00393270
rosa_rugosa	Rorug03G0245500 Rorug04G0069600 Rorug04G0069700 Rorug04G0070700 Rorug04G0070800
rosa_samantha	Rh3AG295700 Rh3BG331900 Rh3CG329000 Rh3DG329500
rosa_wichuraiana	Rw3G026110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2949, 3248
AarI	CACCTGC	1 cut(s)	1264
Acc16I	TGCGCA	1 cut(s)	448
Acc36I	ACCTGC	3 cut(s)	191, 1264, 2467
AccB1I	GGYRCC	2 cut(s)	1967, 2164
AccB7I	CCANNNNNTGG	1 cut(s)	1695
AccBSI	CCGCTC	1 cut(s)	2749
AccI	GTMKAC	1 cut(s)	184
AccII	CGCG	2 cut(s)	476, 1008
AclWI	GGATC	4 cut(s)	703, 1635, 1902, 2291
AcoI	YGGCCR	2 cut(s)	6, 1812
AcuI	CTGAAG	2 cut(s)	297, 3732
AcyI	GRCGYC	2 cut(s)	181, 1968
AdeI	CACNNNGTG	1 cut(s)	365
AfaI	GTAC	8 cut(s)	216, 484, 894, 1136, 1657, 1722, 2610, 3665
AfeI	AGCGCT	1 cut(s)	2702
AfiI	CCNNNNNNNGG	4 cut(s)	410, 1695, 3376, 3780
AflIII	ACRYGT	2 cut(s)	745, 2452
AjiI	CACGTC	1 cut(s)	2453
AjnI	CCWGG	7 cut(s)	176, 289, 488, 535, 1408, 3234, 3739
AjuI	GAANNNNNNNTTGG	4 cut(s)	919, 951, 1200, 1232
Alw21I	GWGCWC	2 cut(s)	124, 3691
Alw26I	GTCTC	6 cut(s)	60, 256, 1235, 2736, 2994, 3270
AlwI	GGATC	4 cut(s)	703, 1635, 1902, 2291
AlwNI	CAGNNNCTG	4 cut(s)	275, 520, 1250, 3047
Ama87I	CYCGRG	1 cut(s)	2612
Aor51HI	AGCGCT	1 cut(s)	2702
AoxI	GGCC	7 cut(s)	6, 127, 457, 1228, 1812, 3043, 3782
ApaI	GGGCCC	1 cut(s)	461
ApeKI	GCWGC	8 cut(s)	194, 197, 625, 2024, 2111, 2209, 2387, 3148
ArsI	GACNNNNNNTTYG	2 cut(s)	2735, 2767
AseI	ATTAAT	2 cut(s)	231, 1578
Asp700I	GAANNNNTTC	1 cut(s)	1458
AspLEI	GCGC	5 cut(s)	449, 630, 1876, 1970, 2703
AspS9I	GGNCC	6 cut(s)	128, 457, 458, 2679, 3044, 3468
AsuC2I	CCSGG	1 cut(s)	52
AsuHPI	GGTGA	9 cut(s)	127, 887, 914, 1532, 1775, 2077, 2084, 2492, 3662
AvaI	CYCGRG	1 cut(s)	2612
AvaII	GGWCC	2 cut(s)	2679, 3468
BaeGI	GKGCMC	2 cut(s)	461, 1299
BaeI	ACNNNNGTAYC	2 cut(s)	1579, 1612
BanI	GGYRCC	2 cut(s)	1967, 2164
BanII	GRGCYC	4 cut(s)	461, 799, 3234, 3691
BauI	CACGAG	3 cut(s)	123, 942, 1910
BbsI	GAAGAC	2 cut(s)	717, 1827
Bbv12I	GWGCWC	2 cut(s)	124, 3691
BbvI	GCAGC	8 cut(s)	181, 184, 612, 2036, 2098, 2221, 2399, 3135
BceAI	ACGGC	3 cut(s)	102, 330, 974
BcgI	CGANNNNNNTGC	2 cut(s)	1358, 1392
BciT130I	CCWGG	7 cut(s)	178, 291, 490, 537, 1410, 3236, 3741
BciVI	GTATCC	1 cut(s)	3160
BclI	TGATCA	2 cut(s)	1339, 3634
BcnI	CCSGG	1 cut(s)	52
BcoDI	GTCTC	6 cut(s)	60, 256, 1235, 2736, 2994, 3270
BfaI	CTAG	5 cut(s)	312, 1232, 1263, 1284, 3255
BfmI	CTRYAG	3 cut(s)	198, 996, 2022
BfoI	RGCGCY	2 cut(s)	1971, 2704
BfuAI	ACCTGC	3 cut(s)	191, 1264, 2467
BfuI	GTATCC	1 cut(s)	3160
BglII	AGATCT	2 cut(s)	3085, 3580
BlpI	GCTNAGC	1 cut(s)	1052
Bme1390I	CCNGG	8 cut(s)	52, 178, 291, 490, 537, 1410, 3236, 3741
Bme18I	GGWCC	2 cut(s)	2679, 3468
BmeT110I	CYCGRG	1 cut(s)	2612
BmgBI	CACGTC	1 cut(s)	2453
BmgT120I	GGNCC	6 cut(s)	128, 457, 458, 2679, 3044, 3468
BmiI	GGNNCC	9 cut(s)	459, 1064, 1494, 1969, 2136, 2166, 2908, 3233, 3470
BmrFI	CCNGG	8 cut(s)	52, 178, 291, 490, 537, 1410, 3236, 3741
BmsI	GCATC	9 cut(s)	889, 1774, 1861, 2626, 2797, 3115, 3385, 3582, 3681
BpiI	GAAGAC	2 cut(s)	717, 1827
BplI	GAGNNNNNCTC	2 cut(s)	1943, 1975
BpmI	CTGGAG	3 cut(s)	527, 558, 3705
Bpu10I	CCTNAGC	1 cut(s)	2148
Bpu1102I	GCTNAGC	1 cut(s)	1052
BpuEI	CTTGAG	4 cut(s)	1724, 2180, 2452, 3675
BpuMI	CCSGG	1 cut(s)	52
Bsa29I	ATCGAT	1 cut(s)	702
BsaBI	GATNNNNATC	1 cut(s)	876
BsaHI	GRCGYC	2 cut(s)	181, 1968
BsaJI	CCNNGG	6 cut(s)	453, 757, 1971, 2529, 3196, 3554
BsaXI	ACNNNNNCTCC	4 cut(s)	255, 285, 2439, 2469
Bsc4I	CCNNNNNNNGG	4 cut(s)	410, 1695, 3376, 3780
Bse1I	ACTGG	6 cut(s)	269, 900, 1018, 1231, 1324, 2593
Bse3DI	GCAATG	5 cut(s)	421, 1284, 2736, 2774, 2789
Bse8I	GATNNNNATC	1 cut(s)	876
BseBI	CCWGG	7 cut(s)	178, 291, 490, 537, 1410, 3236, 3741
BseCI	ATCGAT	1 cut(s)	702
BseDI	CCNNGG	6 cut(s)	453, 757, 1971, 2529, 3196, 3554
BseJI	GATNNNNATC	1 cut(s)	876
BseLI	CCNNNNNNNGG	4 cut(s)	410, 1695, 3376, 3780
BseMI	GCAATG	5 cut(s)	421, 1284, 2736, 2774, 2789
BseMII	CTCAG	7 cut(s)	2070, 2139, 2880, 3005, 3185, 3231, 3483
BseNI	ACTGG	6 cut(s)	269, 900, 1018, 1231, 1324, 2593
BseRI	GAGGAG	2 cut(s)	2885, 3158
BseSI	GKGCMC	2 cut(s)	461, 1299
BseXI	GCAGC	8 cut(s)	181, 184, 612, 2036, 2098, 2221, 2399, 3135
BseYI	CCCAGC	1 cut(s)	1876
BsgI	GTGCAG	3 cut(s)	1236, 1995, 3033
Bsh1236I	CGCG	2 cut(s)	476, 1008
Bsh1285I	CGRYCG	1 cut(s)	803
BshFI	GGCC	7 cut(s)	8, 129, 459, 1230, 1814, 3045, 3784
BshNI	GGYRCC	2 cut(s)	1967, 2164
BshVI	ATCGAT	1 cut(s)	702
BsiEI	CGRYCG	1 cut(s)	803
BsiHKAI	GWGCWC	2 cut(s)	124, 3691
BsiHKCI	CYCGRG	1 cut(s)	2612
BsiSI	CCGG	1 cut(s)	52
BslFI	GGGAC	6 cut(s)	17, 34, 638, 802, 1184, 2873
BslI	CCNNNNNNNGG	4 cut(s)	410, 1695, 3376, 3780
BsmAI	GTCTC	6 cut(s)	60, 256, 1235, 2736, 2994, 3270
BsmBI	CGTCTC	2 cut(s)	60, 2736
BsmFI	GGGAC	6 cut(s)	17, 34, 638, 802, 1184, 2873
BsmI	GAATGC	4 cut(s)	811, 1036, 2583, 2947
BsnI	GGCC	7 cut(s)	8, 129, 459, 1230, 1814, 3045, 3784
BsoBI	CYCGRG	1 cut(s)	2612
Bsp120I	GGGCCC	1 cut(s)	457
Bsp1286I	GDGCHC	6 cut(s)	124, 461, 799, 1299, 3234, 3691
Bsp1407I	TGTACA	2 cut(s)	214, 1720
Bsp1720I	GCTNAGC	1 cut(s)	1052
BspANI	GGCC	7 cut(s)	8, 129, 459, 1230, 1814, 3045, 3784
BspCNI	CTCAG	7 cut(s)	2071, 2140, 2879, 3004, 3184, 3232, 3484
BspDI	ATCGAT	1 cut(s)	702
BspFNI	CGCG	2 cut(s)	476, 1008
BspLI	GGNNCC	9 cut(s)	459, 1064, 1494, 1969, 2136, 2166, 2908, 3233, 3470
BspMAI	CTGCAG	2 cut(s)	202, 2026
BspMI	ACCTGC	3 cut(s)	191, 1264, 2467
BspPI	GGATC	4 cut(s)	703, 1635, 1902, 2291
BspQI	GCTCTTC	1 cut(s)	1074
BspT107I	GGYRCC	2 cut(s)	1967, 2164
BsrBI	CCGCTC	1 cut(s)	2749
BsrDI	GCAATG	5 cut(s)	421, 1284, 2736, 2774, 2789
BsrGI	TGTACA	2 cut(s)	214, 1720
BsrI	ACTGG	6 cut(s)	269, 900, 1018, 1231, 1324, 2593
BssECI	CCNNGG	6 cut(s)	453, 757, 1971, 2529, 3196, 3554
BssNI	GRCGYC	2 cut(s)	181, 1968
BssSI	CACGAG	3 cut(s)	123, 942, 1910
BssT1I	CCWWGG	3 cut(s)	757, 1971, 3554
Bst2BI	CACGAG	3 cut(s)	123, 942, 1910
Bst2UI	CCWGG	7 cut(s)	178, 291, 490, 537, 1410, 3236, 3741
Bst6I	CTCTTC	4 cut(s)	1074, 1538, 1944, 2022
BstACI	GRCGYC	2 cut(s)	181, 1968
BstAPI	GCANNNNNTGC	3 cut(s)	2218, 3038, 3047
BstAUI	TGTACA	2 cut(s)	214, 1720
BstC8I	GCNNGC	7 cut(s)	424, 963, 2219, 2583, 3043, 3400, 3540
BstDSI	CCRYGG	2 cut(s)	453, 3196
BstFNI	CGCG	2 cut(s)	476, 1008
BstH2I	RGCGCY	2 cut(s)	1971, 2704
BstHHI	GCGC	5 cut(s)	449, 630, 1876, 1970, 2703
BstMAI	GTCTC	6 cut(s)	60, 256, 1235, 2736, 2994, 3270
BstMCI	CGRYCG	1 cut(s)	803
BstNI	CCWGG	7 cut(s)	178, 291, 490, 537, 1410, 3236, 3741
BstNSI	RCATGY	6 cut(s)	434, 749, 2060, 2221, 3030, 3402
BstSCI	CCNGG	8 cut(s)	50, 176, 289, 488, 535, 1408, 3234, 3739
BstSFI	CTRYAG	3 cut(s)	198, 996, 2022
BstSLI	GKGCMC	2 cut(s)	461, 1299
BstUI	CGCG	2 cut(s)	476, 1008
BstV1I	GCAGC	8 cut(s)	181, 184, 612, 2036, 2098, 2221, 2399, 3135
BstV2I	GAAGAC	2 cut(s)	717, 1827
BstX2I	RGATCY	2 cut(s)	3085, 3580
BstXI	CCANNNNNNTGG	3 cut(s)	1978, 2695, 3060
BstYI	RGATCY	2 cut(s)	3085, 3580
Bsu15I	ATCGAT	1 cut(s)	702
BsuI	GTATCC	1 cut(s)	3160
BsuRI	GGCC	7 cut(s)	8, 129, 459, 1230, 1814, 3045, 3784
BsuTUI	ATCGAT	1 cut(s)	702
BtgI	CCRYGG	2 cut(s)	453, 3196
BtrI	CACGTC	1 cut(s)	2453
BtsI	GCAGTG	2 cut(s)	2219, 2817
BtsIMutI	CAGTG	5 cut(s)	276, 813, 837, 2219, 2817
BveI	ACCTGC	3 cut(s)	191, 1264, 2467
Cac8I	GCNNGC	7 cut(s)	424, 963, 2219, 2583, 3043, 3400, 3540
CaiI	CAGNNNCTG	4 cut(s)	275, 520, 1250, 3047
CfoI	GCGC	5 cut(s)	449, 630, 1876, 1970, 2703
Cfr13I	GGNCC	6 cut(s)	128, 457, 458, 2679, 3044, 3468
ClaI	ATCGAT	1 cut(s)	702
CseI	GACGC	1 cut(s)	170
Csp6I	GTAC	8 cut(s)	215, 483, 893, 1135, 1656, 1721, 2609, 3664
CviQI	GTAC	8 cut(s)	215, 483, 893, 1135, 1656, 1721, 2609, 3664
DinI	GGCGCC	1 cut(s)	1969
DraI	TTTAAA	1 cut(s)	1357
DraIII	CACNNNGTG	1 cut(s)	365
EaeI	YGGCCR	2 cut(s)	6, 1812
Eam1104I	CTCTTC	4 cut(s)	1074, 1538, 1944, 2022
EarI	CTCTTC	4 cut(s)	1074, 1538, 1944, 2022
Ecl136II	GAGCTC	1 cut(s)	3689
Eco130I	CCWWGG	3 cut(s)	757, 1971, 3554
Eco24I	GRGCYC	4 cut(s)	461, 799, 3234, 3691
Eco32I	GATATC	1 cut(s)	2181
Eco47I	GGWCC	2 cut(s)	2679, 3468
Eco47III	AGCGCT	1 cut(s)	2702
Eco53kI	GAGCTC	1 cut(s)	3689
Eco57I	CTGAAG	2 cut(s)	297, 3732
Eco88I	CYCGRG	1 cut(s)	2612
EcoICRI	GAGCTC	1 cut(s)	3689
EcoO109I	RGGNCCY	1 cut(s)	3044
EcoRI	GAATTC	2 cut(s)	97, 2549
EcoRII	CCWGG	7 cut(s)	176, 289, 488, 535, 1408, 3234, 3739
EcoRV	GATATC	1 cut(s)	2181
EcoT14I	CCWWGG	3 cut(s)	757, 1971, 3554
EcoT22I	ATGCAT	3 cut(s)	2641, 3400, 3674
EcoT38I	GRGCYC	4 cut(s)	461, 799, 3234, 3691
EgeI	GGCGCC	1 cut(s)	1969
EheI	GGCGCC	1 cut(s)	1969
ErhI	CCWWGG	3 cut(s)	757, 1971, 3554
Esp3I	CGTCTC	2 cut(s)	60, 2736
FaqI	GGGAC	6 cut(s)	17, 34, 638, 802, 1184, 2873
FauI	CCCGC	1 cut(s)	663
FauNDI	CATATG	5 cut(s)	735, 1516, 2604, 3295, 3631
FbaI	TGATCA	2 cut(s)	1339, 3634
FblI	GTMKAC	1 cut(s)	184
FriOI	GRGCYC	4 cut(s)	461, 799, 3234, 3691
FspBI	CTAG	5 cut(s)	312, 1232, 1263, 1284, 3255
FspI	TGCGCA	1 cut(s)	448
GlaI	GCGC	5 cut(s)	448, 629, 1875, 1969, 2702
GsaI	CCCAGC	1 cut(s)	1880
GsuI	CTGGAG	3 cut(s)	527, 558, 3705
HaeII	RGCGCY	2 cut(s)	1971, 2704
HaeIII	GGCC	7 cut(s)	8, 129, 459, 1230, 1814, 3045, 3784
HapII	CCGG	1 cut(s)	52
HgaI	GACGC	1 cut(s)	170
HhaI	GCGC	5 cut(s)	449, 630, 1876, 1970, 2703
Hin1I	GRCGYC	2 cut(s)	181, 1968
Hin6I	GCGC	5 cut(s)	447, 628, 1874, 1968, 2701
HinP1I	GCGC	5 cut(s)	447, 628, 1874, 1968, 2701
HincII	GTYRAC	2 cut(s)	1012, 1957
HindII	GTYRAC	2 cut(s)	1012, 1957
HindIII	AAGCTT	3 cut(s)	1049, 1301, 2692
HpaII	CCGG	1 cut(s)	52
HphI	GGTGA	9 cut(s)	127, 887, 914, 1532, 1775, 2077, 2084, 2492, 3662
Hpy188III	TCNNGA	8 cut(s)	506, 1066, 1641, 1946, 2243, 2894, 3004, 3119
Hpy99I	CGWCG	2 cut(s)	342, 345
HpyAV	CCTTC	9 cut(s)	1053, 1638, 1643, 1728, 2985, 3169, 3370, 3482, 3553
HpyCH4IV	ACGT	3 cut(s)	30, 772, 2452
HpySE526I	ACGT	3 cut(s)	30, 772, 2452
Hsp92I	GRCGYC	2 cut(s)	181, 1968
HspAI	GCGC	5 cut(s)	447, 628, 1874, 1968, 2701
KasI	GGCGCC	1 cut(s)	1967
Ksp22I	TGATCA	2 cut(s)	1339, 3634
LguI	GCTCTTC	1 cut(s)	1074
LmnI	GCTCC	7 cut(s)	517, 1264, 2473, 2698, 3145, 3237, 3686
Lsp1109I	GCAGC	8 cut(s)	181, 184, 612, 2036, 2098, 2221, 2399, 3135
LweI	GCATC	9 cut(s)	889, 1774, 1861, 2626, 2797, 3115, 3385, 3582, 3681
MaeI	CTAG	5 cut(s)	312, 1232, 1263, 1284, 3255
MaeII	ACGT	3 cut(s)	30, 772, 2452
MbiI	CCGCTC	1 cut(s)	2749
MfeI	CAATTG	3 cut(s)	1617, 2657, 3638
MflI	RGATCY	2 cut(s)	3085, 3580
MhlI	GDGCHC	6 cut(s)	124, 461, 799, 1299, 3234, 3691
Mly113I	GGCGCC	1 cut(s)	1968
MlyI	GAGTC	5 cut(s)	1631, 2531, 2891, 3104, 3272
MmeI	TCCRAC	4 cut(s)	197, 537, 1959, 3432
Mph1103I	ATGCAT	3 cut(s)	2641, 3400, 3674
MroXI	GAANNNNTTC	1 cut(s)	1458
MslI	CAYNNNNRTG	2 cut(s)	435, 3123
MspA1I	CMGCKG	1 cut(s)	2390
MspI	CCGG	1 cut(s)	52
MspR9I	CCNGG	8 cut(s)	52, 178, 291, 490, 537, 1410, 3236, 3741
MunI	CAATTG	3 cut(s)	1617, 2657, 3638
Mva1269I	GAATGC	4 cut(s)	811, 1036, 2583, 2947
MvaI	CCWGG	7 cut(s)	178, 291, 490, 537, 1410, 3236, 3741
MvnI	CGCG	2 cut(s)	476, 1008
NarI	GGCGCC	1 cut(s)	1968
NciI	CCSGG	1 cut(s)	52
NdeI	CATATG	5 cut(s)	735, 1516, 2604, 3295, 3631
NlaIV	GGNNCC	9 cut(s)	459, 1064, 1494, 1969, 2136, 2166, 2908, 3233, 3470
NmuCI	GTSAC	7 cut(s)	271, 374, 1401, 1520, 1634, 1660, 2254
NsbI	TGCGCA	1 cut(s)	448
NsiI	ATGCAT	3 cut(s)	2641, 3400, 3674
NspI	RCATGY	6 cut(s)	434, 749, 2060, 2221, 3030, 3402
PaeI	GCATGC	2 cut(s)	2221, 3402
PaeR7I	CTCGAG	1 cut(s)	2612
PaqCI	CACCTGC	1 cut(s)	1264
PciI	ACATGT	1 cut(s)	745
PciSI	GCTCTTC	1 cut(s)	1074
PcsI	WCGNNNNNNNCGW	2 cut(s)	121, 343
PctI	GAATGC	4 cut(s)	811, 1036, 2583, 2947
PdmI	GAANNNNTTC	1 cut(s)	1458
PflMI	CCANNNNNTGG	1 cut(s)	1695
PfoI	TCCNGGA	5 cut(s)	50, 289, 535, 1408, 3739
Ple19I	CGATCG	1 cut(s)	803
PleI	GAGTC	5 cut(s)	1631, 2530, 2890, 3103, 3272
PluTI	GGCGCC	1 cut(s)	1971
PpsI	GAGTC	5 cut(s)	1631, 2530, 2890, 3103, 3272
PscI	ACATGT	1 cut(s)	745
PshBI	ATTAAT	2 cut(s)	231, 1578
PsiI	TTATAA	2 cut(s)	2949, 3248
Psp124BI	GAGCTC	1 cut(s)	3691
Psp6I	CCWGG	7 cut(s)	176, 289, 488, 535, 1408, 3234, 3739
PspFI	CCCAGC	1 cut(s)	1876
PspGI	CCWGG	7 cut(s)	176, 289, 488, 535, 1408, 3234, 3739
PspN4I	GGNNCC	9 cut(s)	459, 1064, 1494, 1969, 2136, 2166, 2908, 3233, 3470
PspOMI	GGGCCC	1 cut(s)	457
PspPI	GGNCC	6 cut(s)	128, 457, 458, 2679, 3044, 3468
PspXI	VCTCGAGB	1 cut(s)	2612
PstI	CTGCAG	2 cut(s)	202, 2026
PstNI	CAGNNNCTG	4 cut(s)	275, 520, 1250, 3047
PsuI	RGATCY	2 cut(s)	3085, 3580
PvuI	CGATCG	1 cut(s)	803
PvuII	CAGCTG	1 cut(s)	2390
RsaI	GTAC	8 cut(s)	216, 484, 894, 1136, 1657, 1722, 2610, 3665
RsaNI	GTAC	8 cut(s)	215, 483, 893, 1135, 1656, 1721, 2609, 3664
RseI	CAYNNNNRTG	2 cut(s)	435, 3123
SacI	GAGCTC	1 cut(s)	3691
SapI	GCTCTTC	1 cut(s)	1074
Sau96I	GGNCC	6 cut(s)	128, 457, 458, 2679, 3044, 3468
SchI	GAGTC	5 cut(s)	1631, 2531, 2891, 3104, 3272
ScrFI	CCNGG	8 cut(s)	52, 178, 291, 490, 537, 1410, 3236, 3741
SduI	GDGCHC	6 cut(s)	124, 461, 799, 1299, 3234, 3691
SfaNI	GCATC	9 cut(s)	889, 1774, 1861, 2626, 2797, 3115, 3385, 3582, 3681
SfcI	CTRYAG	3 cut(s)	198, 996, 2022
SfoI	GGCGCC	1 cut(s)	1969
Sfr274I	CTCGAG	1 cut(s)	2612
SinI	GGWCC	2 cut(s)	2679, 3468
SlaI	CTCGAG	1 cut(s)	2612
SmiMI	CAYNNNNRTG	2 cut(s)	435, 3123
SmlI	CTYRAG	5 cut(s)	1739, 2159, 2467, 2612, 3690
SmoI	CTYRAG	5 cut(s)	1739, 2159, 2467, 2612, 3690
SphI	GCATGC	2 cut(s)	2221, 3402
SspDI	GGCGCC	1 cut(s)	1967
SspI	AATATT	1 cut(s)	1551
SspMI	CTAG	5 cut(s)	312, 1232, 1263, 1284, 3255
SstI	GAGCTC	1 cut(s)	3691
StyD4I	CCNGG	8 cut(s)	50, 176, 289, 488, 535, 1408, 3234, 3739
StyI	CCWWGG	3 cut(s)	757, 1971, 3554
TaiI	ACGT	3 cut(s)	33, 775, 2455
TaqII	GACCGA	1 cut(s)	2667
TatI	WGTACW	6 cut(s)	214, 482, 892, 1655, 1720, 3663
TauI	GCSGC	6 cut(s)	8, 94, 479, 1817, 2365, 2588
TscAI	CASTG	5 cut(s)	276, 820, 837, 2219, 2817
TseFI	GTSAC	7 cut(s)	271, 374, 1401, 1520, 1634, 1660, 2254
TseI	GCWGC	8 cut(s)	194, 197, 625, 2024, 2111, 2209, 2387, 3148
Tsp45I	GTSAC	7 cut(s)	271, 374, 1401, 1520, 1634, 1660, 2254
TspGWI	ACGGA	8 cut(s)	32, 36, 876, 1324, 2543, 2754, 3029, 3185
TspRI	CASTG	5 cut(s)	276, 820, 837, 2219, 2817
Van91I	CCANNNNNTGG	1 cut(s)	1695
VpaK11BI	GGWCC	2 cut(s)	2679, 3468
VspI	ATTAAT	2 cut(s)	231, 1578
XceI	RCATGY	6 cut(s)	434, 749, 2060, 2221, 3030, 3402
XhoI	CTCGAG	1 cut(s)	2612
XmiI	GTMKAC	1 cut(s)	184
XmnI	GAANNNNTTC	1 cut(s)	1458
XspI	CTAG	5 cut(s)	312, 1232, 1263, 1284, 3255
Zsp2I	ATGCAT	3 cut(s)	2641, 3400, 3674

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.