Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0003778

Physical Location & Seq

Genomic Coordinates Forward (+)

10887793 .. 10889115

Gene Structure 1323 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0070700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1308 bp

ATGGATCTCGACTGCACCAGAAAGAGAAGATCGTCACCACCAATTTGGTCTAAGCTTCCACCTGAAATATTAGGGTTCATCACGACAAAGCTTGGTTCGGTAGACACCATTCGCTTTAAAGTCGTTTGCCCTTCTTGGAATCGAGCAGCCAAATCTTACATCTCTTCTCCATGTTACAAAGTCATGGAGCAAACTCCGTTGCTCATGATCCCTAGCCAAGAAAAGAAGGATCTCCATACTCGCCGCTTCTTCAGACTTGCAGAGAACAATACTTTCACTCTCAAAAACGTGTTTCGAGATTTTCCGGAGGCTTGGTGTGTGGGTTCATCACACGGATGGCTGGTGCTTATGGATGGGACAGGAAAACTGGATCTTTTTAATCCGTTTACTGAAGATAGGATTAAACTCCCATCGCTTTGGACTCTTCCTCACTTTGCCAAAATTAAAACTCACCCCAACTTCTCCGATGAGTCGTTCGCGCGCAATACCAATTTACTGAAGGGCTATATGATGGGAAAAGCTGTCACTTCTTGTAACCCGTCTTGCAACAAGGACTTTGCTGTTGTGATAACTCACAACTTGCGAGGAAGACTTGCATTTTGTAAGCATGGGAACAACAGATGTACATGGACTGACCTAGGAGACGAGTCTGATCACTATAGTGATATTATCTTCCACAATGGCCATTTATACACATTGACATCCGGTGGTTCAGTTGGAGTTTGGGACATGACGAAATCTTTTCCCACCAAAATTTTTGATCTTCACCCTTTTGCAGACCAGTCTGAGATAAATAAAGATTTTCCCTTTGCCAAATTTGATGTTCAACATTATTTGGTGGAGTCGTCGGGCGACCTTTTATTTGTTTGGCGATTTATAGTGAATACTATTGAAGGCGAAACGTTCCCATATAGAACCTTGCACTTTCATATCTTTAGGCTGAGTTCCACTAGTAAAGAGTGGGAGAAAGTGGAATATTTGCATGACCGGGCTCTGTTTTTGGGAGGCAATCATTCAATGTCAGTATCCTCCCGAGTCTTTCCTGAGTGTCAAGAAAACTCGATTTACTTTACAGATGATAATTCTTATGAGATGAGTTTAAAGTACATTGCTAATGGTCAGGAAGGCGGTCATGATATAGGAGTATACAATTTAAAAGATAAGGTTGTCGAGCCATTACCAGGTCACCAATATTTTGATAAGTGGACAATTTATGATCCACTTCCCTTCTGGATTTTTCCCAACCCTTGGCCTTCCACAGTGAGCTACAATGCAGCATCACCCTCGCTATCAGGGCCACGACCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0007275 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M16 Peptidase_M16_C

Protein Analysis

435

Amino Acids

50.02

Weight (kDa)

7.26

Isoelectric Point (pI)

43.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_KIB1-4	PF03478	83 - 384	5.8e-62	KIB1-4 beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003778)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42390 AT5G42390
fragaria_vesca	FvH4_1g30291 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800
malus_domestica	MD00G1159400.v1.1 MD00G1204700.v1.1
prunus_persica	Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1
pyrus_communis	pycom290g00010
rosa_chinensis	RchiOBHm_Chr3g0491051
rosa_laevigata	RLG00000022866
rosa_multiflora	Rmu_co8044182.1_g000001 Rmu_ssc0000386.1_g000043
rosa_roxburghii	Rroxscaffold_5G00351210 Rroxscaffold_6G00393270
rosa_rugosa	Rorug03G0245500 Rorug04G0069600 Rorug04G0069700 Rorug04G0070700 Rorug04G0070800
rosa_samantha	Rh3AG295700 Rh3BG331900 Rh3CG329000 Rh3DG329500
rosa_wichuraiana	Rw3G026110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1248
AccI	GTMKAC	2 cut(s)	102, 1146
AccII	CGCG	2 cut(s)	479, 481
AccIII	TCCGGA	1 cut(s)	304
AciI	CCGC	2 cut(s)	244, 1128
AclI	AACGTT	1 cut(s)	902
AclWI	GGATC	5 cut(s)	12, 202, 237, 378, 1211
AcoI	YGGCCR	1 cut(s)	682
AcsI	RAATTY	2 cut(s)	753, 815
AcuI	CTGAAG	3 cut(s)	235, 411, 518
AfaI	GTAC	2 cut(s)	625, 1106
AfiI	CCNNNNNNNGG	2 cut(s)	1181, 1248
AflIII	ACRYGT	1 cut(s)	288
AgsI	TTSAA	3 cut(s)	827, 893, 1017
AhlI	ACTAGT	1 cut(s)	950
AjnI	CCWGG	1 cut(s)	1180
AleI	CACNNNNGTG	1 cut(s)	660
AluBI	AGCT	4 cut(s)	55, 91, 521, 1266
AluI	AGCT	4 cut(s)	55, 91, 521, 1266
Alw26I	GTCTC	1 cut(s)	636
AlwI	GGATC	5 cut(s)	12, 202, 237, 378, 1211
Ama87I	CYCGRG	1 cut(s)	1032
Aor13HI	TCCGGA	1 cut(s)	304
AoxI	GGCC	3 cut(s)	682, 1250, 1295
ApeKI	GCWGC	2 cut(s)	146, 1274
ApoI	RAATTY	2 cut(s)	753, 815
ArsI	GACNNNNNNTTYG	4 cut(s)	582, 614, 845, 877
AspA2I	CCTAGG	1 cut(s)	637
AspLEI	GCGC	2 cut(s)	481, 483
AspS9I	GGNCC	1 cut(s)	1295
AsuC2I	CCSGG	1 cut(s)	989
AsuHPI	GGTGA	5 cut(s)	27, 443, 758, 1178, 1272
AvaI	CYCGRG	1 cut(s)	1032
AvrII	CCTAGG	1 cut(s)	637
BalI	TGGCCA	1 cut(s)	684
BanII	GRGCYC	1 cut(s)	994
BbsI	GAAGAC	1 cut(s)	595
BbvI	GCAGC	2 cut(s)	158, 1286
BccI	CCATC	4 cut(s)	330, 347, 418, 505
BcgI	CGANNNNNNTGC	2 cut(s)	1266, 1300
BciT130I	CCWGG	1 cut(s)	1182
BciVI	GTATCC	1 cut(s)	1036
BclI	TGATCA	1 cut(s)	652
BcnI	CCSGG	1 cut(s)	989
BcoDI	GTCTC	1 cut(s)	636
BcuI	ACTAGT	1 cut(s)	950
BfaI	CTAG	3 cut(s)	213, 638, 951
BfmI	CTRYAG	1 cut(s)	658
BfuI	GTATCC	1 cut(s)	1036
BisI	GCNGC	3 cut(s)	147, 244, 1275
BlnI	CCTAGG	1 cut(s)	637
BlsI	GCNGC	3 cut(s)	148, 245, 1276
Bme1390I	CCNGG	2 cut(s)	989, 1182
BmeT110I	CYCGRG	1 cut(s)	1032
BmgT120I	GGNCC	1 cut(s)	1295
BmrFI	CCNGG	2 cut(s)	989, 1182
BmsI	GCATC	1 cut(s)	1286
BpiI	GAAGAC	1 cut(s)	595
BpuMI	CCSGG	1 cut(s)	989
BsaJI	CCNNGG	2 cut(s)	637, 1247
BsaWI	WCCGGW	2 cut(s)	304, 704
BsaXI	ACNNNNNCTCC	2 cut(s)	299, 329
Bsc4I	CCNNNNNNNGG	2 cut(s)	1181, 1248
Bse1I	ACTGG	2 cut(s)	372, 781
Bse3DI	GCAATG	1 cut(s)	1107
BseAI	TCCGGA	1 cut(s)	304
BseBI	CCWGG	1 cut(s)	1182
BseDI	CCNNGG	2 cut(s)	637, 1247
BseGI	GGATG	3 cut(s)	341, 358, 701
BseLI	CCNNNNNNNGG	2 cut(s)	1181, 1248
BseMI	GCAATG	1 cut(s)	1107
BseMII	CTCAG	3 cut(s)	777, 932, 1035
BseNI	ACTGG	2 cut(s)	372, 781
BsePI	GCGCGC	1 cut(s)	479
BseXI	GCAGC	2 cut(s)	158, 1286
Bsh1236I	CGCG	2 cut(s)	479, 481
BshFI	GGCC	3 cut(s)	684, 1252, 1297
BsiHKCI	CYCGRG	1 cut(s)	1032
BsiSI	CCGG	3 cut(s)	305, 705, 988
BslFI	GGGAC	2 cut(s)	370, 740
BslI	CCNNNNNNNGG	2 cut(s)	1181, 1248
BsmAI	GTCTC	1 cut(s)	636
BsmBI	CGTCTC	1 cut(s)	636
BsmFI	GGGAC	2 cut(s)	370, 740
BsnI	GGCC	3 cut(s)	684, 1252, 1297
BsoBI	CYCGRG	1 cut(s)	1032
Bsp1286I	GDGCHC	1 cut(s)	994
Bsp13I	TCCGGA	1 cut(s)	304
Bsp1407I	TGTACA	1 cut(s)	623
Bsp143I	GATC	8 cut(s)	4, 29, 207, 229, 370, 652, 760, 1216
BspACI	CCGC	2 cut(s)	244, 1128
BspANI	GGCC	3 cut(s)	684, 1252, 1297
BspCNI	CTCAG	3 cut(s)	778, 933, 1036
BspEI	TCCGGA	1 cut(s)	304
BspFNI	CGCG	2 cut(s)	479, 481
BspHI	TCATGA	2 cut(s)	204, 1132
BspPI	GGATC	5 cut(s)	12, 202, 237, 378, 1211
BsrDI	GCAATG	1 cut(s)	1107
BsrGI	TGTACA	1 cut(s)	623
BsrI	ACTGG	2 cut(s)	372, 781
BssECI	CCNNGG	2 cut(s)	637, 1247
BssHII	GCGCGC	1 cut(s)	479
BssMI	GATC	8 cut(s)	4, 29, 207, 229, 370, 652, 760, 1216
BssNAI	GTATAC	1 cut(s)	1147
BssT1I	CCWWGG	2 cut(s)	637, 1247
Bst1107I	GTATAC	1 cut(s)	1147
Bst2UI	CCWGG	1 cut(s)	1182
Bst4CI	ACNGT	1 cut(s)	1261
Bst6I	CTCTTC	2 cut(s)	169, 429
BstAUI	TGTACA	1 cut(s)	623
BstC8I	GCNNGC	1 cut(s)	481
BstDEI	CTNAG	4 cut(s)	51, 786, 941, 1044
BstEII	GGTNACC	1 cut(s)	1184
BstF5I	GGATG	3 cut(s)	341, 358, 701
BstFNI	CGCG	2 cut(s)	479, 481
BstHHI	GCGC	2 cut(s)	481, 483
BstKTI	GATC	8 cut(s)	7, 32, 210, 232, 373, 655, 763, 1219
BstMAI	GTCTC	1 cut(s)	636
BstMBI	GATC	8 cut(s)	4, 29, 207, 229, 370, 652, 760, 1216
BstMWI	GCNNNNNNNGC	1 cut(s)	1294
BstNI	CCWGG	1 cut(s)	1182
BstPI	GGTNACC	1 cut(s)	1184
BstSCI	CCNGG	2 cut(s)	987, 1180
BstSFI	CTRYAG	1 cut(s)	658
BstUI	CGCG	2 cut(s)	479, 481
BstV1I	GCAGC	2 cut(s)	158, 1286
BstV2I	GAAGAC	1 cut(s)	595
BstX2I	RGATCY	3 cut(s)	4, 229, 370
BstXI	CCANNNNNNTGG	2 cut(s)	45, 417
BstYI	RGATCY	3 cut(s)	4, 229, 370
BstZ17I	GTATAC	1 cut(s)	1147
BsuI	GTATCC	1 cut(s)	1036
BsuRI	GGCC	3 cut(s)	684, 1252, 1297
BtgZI	GCGATG	1 cut(s)	396
BtsCI	GGATG	3 cut(s)	341, 358, 701
BtsIMutI	CAGTG	1 cut(s)	1266
Cac8I	GCNNGC	1 cut(s)	481
CciI	TCATGA	2 cut(s)	204, 1132
CfoI	GCGC	2 cut(s)	481, 483
Cfr13I	GGNCC	1 cut(s)	1295
CsiI	ACCWGGT	1 cut(s)	1180
Csp6I	GTAC	2 cut(s)	624, 1105
CviAII	CATG	8 cut(s)	171, 184, 205, 608, 627, 730, 983, 1133
CviQI	GTAC	2 cut(s)	624, 1105
DdeI	CTNAG	4 cut(s)	51, 786, 941, 1044
DpnI	GATC	8 cut(s)	6, 31, 209, 231, 372, 654, 762, 1218
DpnII	GATC	8 cut(s)	4, 29, 207, 229, 370, 652, 760, 1216
DraI	TTTAAA	3 cut(s)	118, 1101, 1155
EaeI	YGGCCR	1 cut(s)	682
Eam1104I	CTCTTC	2 cut(s)	169, 429
EarI	CTCTTC	2 cut(s)	169, 429
Eco130I	CCWWGG	2 cut(s)	637, 1247
Eco24I	GRGCYC	1 cut(s)	994
Eco57I	CTGAAG	3 cut(s)	235, 411, 518
Eco88I	CYCGRG	1 cut(s)	1032
Eco91I	GGTNACC	1 cut(s)	1184
EcoO65I	GGTNACC	1 cut(s)	1184
EcoRII	CCWGG	1 cut(s)	1180
EcoT14I	CCWWGG	2 cut(s)	637, 1247
EcoT38I	GRGCYC	1 cut(s)	994
ErhI	CCWWGG	2 cut(s)	637, 1247
Esp3I	CGTCTC	1 cut(s)	636
FaeI	CATG	8 cut(s)	174, 187, 208, 611, 630, 733, 986, 1136
FaqI	GGGAC	2 cut(s)	370, 740
FatI	CATG	8 cut(s)	170, 183, 204, 607, 626, 729, 982, 1132
FbaI	TGATCA	1 cut(s)	652
FblI	GTMKAC	2 cut(s)	102, 1146
Fnu4HI	GCNGC	3 cut(s)	147, 244, 1275
FokI	GGATG	3 cut(s)	348, 365, 688
FriOI	GRGCYC	1 cut(s)	994
Fsp4HI	GCNGC	3 cut(s)	147, 244, 1275
FspBI	CTAG	3 cut(s)	213, 638, 951
GlaI	GCGC	2 cut(s)	480, 482
GluI	GCNGC	3 cut(s)	147, 244, 1275
HaeIII	GGCC	3 cut(s)	684, 1252, 1297
HapII	CCGG	3 cut(s)	305, 705, 988
HhaI	GCGC	2 cut(s)	481, 483
Hin1II	CATG	8 cut(s)	174, 187, 208, 611, 630, 733, 986, 1136
Hin6I	GCGC	2 cut(s)	479, 481
HinP1I	GCGC	2 cut(s)	479, 481
HindIII	AAGCTT	2 cut(s)	53, 89
HinfI	GANTC	6 cut(s)	139, 421, 470, 647, 842, 1035
HpaII	CCGG	3 cut(s)	305, 705, 988
HphI	GGTGA	5 cut(s)	27, 443, 758, 1178, 1272
Hpy166II	GTNNAC	4 cut(s)	103, 387, 1147, 1206
Hpy188I	TCNGA	4 cut(s)	254, 466, 652, 787
Hpy8I	GTNNAC	4 cut(s)	103, 387, 1147, 1206
Hpy99I	CGWCG	1 cut(s)	850
HpyAV	CCTTC	7 cut(s)	141, 220, 493, 887, 1118, 1237, 1263
HpyCH4III	ACNGT	1 cut(s)	1261
HpyCH4IV	ACGT	2 cut(s)	288, 902
HpyCH4V	TGCA	8 cut(s)	15, 260, 546, 596, 776, 922, 982, 1274
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1294
HpyF3I	CTNAG	4 cut(s)	51, 786, 941, 1044
HpySE526I	ACGT	2 cut(s)	288, 902
Hsp92II	CATG	8 cut(s)	174, 187, 208, 611, 630, 733, 986, 1136
HspAI	GCGC	2 cut(s)	479, 481
Kpn2I	TCCGGA	1 cut(s)	304
Ksp22I	TGATCA	1 cut(s)	652
Kzo9I	GATC	8 cut(s)	4, 29, 207, 229, 370, 652, 760, 1216
LmnI	GCTCC	1 cut(s)	187
Lsp1109I	GCAGC	2 cut(s)	158, 1286
LweI	GCATC	1 cut(s)	1286
MabI	ACCWGGT	1 cut(s)	1180
MaeI	CTAG	3 cut(s)	213, 638, 951
MaeII	ACGT	2 cut(s)	288, 902
MaeIII	GTNAC	5 cut(s)	33, 173, 523, 533, 1184
MalI	GATC	8 cut(s)	6, 31, 209, 231, 372, 654, 762, 1218
MboI	GATC	8 cut(s)	4, 29, 207, 229, 370, 652, 760, 1216
MboII	GAAGA	8 cut(s)	39, 156, 241, 404, 416, 600, 664, 755
MflI	RGATCY	3 cut(s)	4, 229, 370
MhlI	GDGCHC	1 cut(s)	994
MlsI	TGGCCA	1 cut(s)	684
MluCI	AATT	8 cut(s)	42, 441, 490, 753, 815, 1081, 1150, 1209
MluNI	TGGCCA	1 cut(s)	684
MlyI	GAGTC	5 cut(s)	415, 479, 656, 851, 1044
MmeI	TCCRAC	1 cut(s)	697
MnlI	CCTC	6 cut(s)	301, 438, 578, 998, 1039, 1294
Mox20I	TGGCCA	1 cut(s)	684
MroI	TCCGGA	1 cut(s)	304
MscI	TGGCCA	1 cut(s)	684
MseI	TTAA	6 cut(s)	117, 378, 402, 444, 1100, 1154
MslI	CAYNNNNRTG	2 cut(s)	334, 660
Msp20I	TGGCCA	1 cut(s)	684
MspI	CCGG	3 cut(s)	305, 705, 988
MspR9I	CCNGG	2 cut(s)	989, 1182
MvaI	CCWGG	1 cut(s)	1182
MvnI	CGCG	2 cut(s)	479, 481
MwoI	GCNNNNNNNGC	1 cut(s)	1294
NciI	CCSGG	1 cut(s)	989
NdeII	GATC	8 cut(s)	4, 29, 207, 229, 370, 652, 760, 1216
NlaIII	CATG	8 cut(s)	174, 187, 208, 611, 630, 733, 986, 1136
NmuCI	GTSAC	3 cut(s)	33, 523, 1184
OliI	CACNNNNGTG	1 cut(s)	660
PagI	TCATGA	2 cut(s)	204, 1132
PauI	GCGCGC	1 cut(s)	479
PfeI	GAWTC	1 cut(s)	139
PflMI	CCANNNNNTGG	1 cut(s)	1248
PkrI	GCNGC	3 cut(s)	148, 245, 1276
PleI	GAGTC	5 cut(s)	415, 478, 655, 850, 1043
PpsI	GAGTC	5 cut(s)	415, 478, 655, 850, 1043
Psp1406I	AACGTT	1 cut(s)	902
Psp6I	CCWGG	1 cut(s)	1180
PspEI	GGTNACC	1 cut(s)	1184
PspGI	CCWGG	1 cut(s)	1180
PspPI	GGNCC	1 cut(s)	1295
PsuI	RGATCY	3 cut(s)	4, 229, 370
PteI	GCGCGC	1 cut(s)	479
RsaI	GTAC	2 cut(s)	625, 1106
RsaNI	GTAC	2 cut(s)	624, 1105
RseI	CAYNNNNRTG	2 cut(s)	334, 660
SaqAI	TTAA	6 cut(s)	117, 378, 402, 444, 1100, 1154
SatI	GCNGC	3 cut(s)	147, 244, 1275
Sau3AI	GATC	8 cut(s)	4, 29, 207, 229, 370, 652, 760, 1216
Sau96I	GGNCC	1 cut(s)	1295
SchI	GAGTC	5 cut(s)	415, 479, 656, 851, 1044
ScrFI	CCNGG	2 cut(s)	989, 1182
SduI	GDGCHC	1 cut(s)	994
SexAI	ACCWGGT	1 cut(s)	1180
SfaNI	GCATC	1 cut(s)	1286
SfcI	CTRYAG	1 cut(s)	658
SmiMI	CAYNNNNRTG	2 cut(s)	334, 660
SpeI	ACTAGT	1 cut(s)	950
Sse9I	AATT	8 cut(s)	42, 441, 490, 753, 815, 1081, 1150, 1209
SsiI	CCGC	2 cut(s)	244, 1128
SspI	AATATT	3 cut(s)	69, 977, 1193
SspMI	CTAG	3 cut(s)	213, 638, 951
StyD4I	CCNGG	2 cut(s)	987, 1180
StyI	CCWWGG	2 cut(s)	637, 1247
TaaI	ACNGT	1 cut(s)	1261
TaiI	ACGT	2 cut(s)	291, 905
TaqI	TCGA	5 cut(s)	9, 142, 295, 1061, 1170
TasI	AATT	8 cut(s)	42, 441, 490, 753, 815, 1081, 1150, 1209
TatI	WGTACW	2 cut(s)	623, 1104
TauI	GCSGC	1 cut(s)	246
TfiI	GAWTC	1 cut(s)	139
Tru1I	TTAA	6 cut(s)	117, 378, 402, 444, 1100, 1154
Tru9I	TTAA	6 cut(s)	117, 378, 402, 444, 1100, 1154
TscAI	CASTG	1 cut(s)	1266
TseFI	GTSAC	3 cut(s)	33, 523, 1184
TseI	GCWGC	2 cut(s)	146, 1274
Tsp45I	GTSAC	3 cut(s)	33, 523, 1184
TspDTI	ATGAA	3 cut(s)	67, 315, 917
TspGWI	ACGGA	3 cut(s)	186, 348, 372
TspRI	CASTG	1 cut(s)	1266
Van91I	CCANNNNNTGG	1 cut(s)	1248
XapI	RAATTY	2 cut(s)	753, 815
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1227
XmaJI	CCTAGG	1 cut(s)	637
XmiI	GTMKAC	2 cut(s)	102, 1146
XspI	CTAG	3 cut(s)	213, 638, 951

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.