Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0003778

Physical Location & Seq

Genomic Coordinates Reverse (-)

35241076 .. 35253026

Gene Structure 11951 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1159400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3771 bp

ATGGCGGCCATAAGCTCTCTTGTTGTAAACGTCCCCCTAATCCGTCCGTCCCAGAGAATAAGCAGAGACGACGACACTTATTCGAATAGGCGGCTGAATTCGGTGTTCTGTGTTCGCAGTGCTCGTGGCCCTCTCACCCTTTTGCATATCAATCGTCTGTCTTTATTTTCTCCAACCAGGCGTCTACTTGAAGCTGGTGCAGGTAGATCAGGTGTACATAGAAAGAAAATTAATACTTGGGAACAATGTGTCTCCATGCTTGGAGAGCCAGTGACTGATGCACTTTTTCCAGGAAAGTATAACTGCATTACTAGCTCTTTTTGCCAAAACAGAAGTCGTCATCACTGTAGGACATCTACACCAAGGGTCTATAATGACAAAACTTCTTTTTGTTTATTCAAGCCTAAACTTGGAAATGTTGCTGGCAAACATGCTCATGTTTCATATGCAACCGTGGGCCCAGATGAGCCACATGCAGCAAGTACAGCCTGGCAAGATGGTCTTCTGGAGAAACAGGAGCTGGATTTATTATATCCTGGAGTAGAAAAAACAGAGTTAGAGGGGTTTTTAAGTTCTGAAATTCCATCTCATCCAAAGTTGTATCGAGGGCAACTAAAAAATGGGCTGCGCTATGTGATCTTACCAAATAAAGTTCCGCCAAACAGGTTTGAAGCACACATGGAAGTTCATTCAGGATCAATTGATGAGCAAGACGATGAGCAAGGAATTGCACATATGATTGAACATGTTGCTTTCCTTGGAAGTAAGAAACGTGAGAAACTTCTTGGGACAGGGGCTCGATCTAATGCTTACACTGACTTCCATCATACAGTGTTTCACATCCATTCGCCCATTAGTTCAAAGGATTCTGATGGTGATCTGCTTCCATATGTACTGGATGCCTTGAATGAGATTGCTTTTCACCCGAAATTCCTTTCTTCTCGTGTTGAAAAAGAACGGCATGCGATTCTGTCAGAATTACAGATGATGAATACTATAGATTATCGAGTTGACTGCCAGTTATTACAACATCTGCATTCTGAAAACAAGCTTAGCAAAAGGTTCCCGATTGGGTTAGAAGAGCAGATTAAGAAGTGGGATGTAGATAAAATAAGGAAATTCCATGAGCGTTGGTACTTCCCTGCAAATGCAACCTTATACATTGTTGGGGACATTGATAACATCTCAAAAACGATTTACCAGATTGAAGCTGTCTTTGGACAAACTGGCCTAGAAAATAAGACAGTTCCTGCACCTGCTCCTAGTGCTTTTGGTGCAATGGCTAGTTTCCTTGTGCCCAAGCTTTCAGTTGGGCTGACTGGAAGTTCTTCTAATTCCGTTGATCAATCTAAAATCTTTAAAAAGGAAAGGCACACAGTTCGTCTTCCTGTGAAACATAATTGGTCACTTCCTGGAAGTAATATGGATTTGAAGCCTCCACAGATTTTTCAGCATGAATTGATTCAAAATTTTTCGTTTAATATGGTTCAAACATATGGTGACTTGCGAAATGTTCTAATGAAGAGAATATTTCTGTCTGCTTTGCATTTCCGAATTAATACAAGATACAAGAGTTCAAATCCACCATTTACTTCAATTGAATTGGATCATAGTGACTCTGGAAGGGAAGGATGTACTGTCACTACTCTTACGGTAACTGCCGAACCCAAGAATTGGCAAGGTGCAATTAGAGTTGCTGTACAAGAGGTTAGAAGGCTCAAGGAATTTGGTGTTACTAAGGGTGAATTAACTCGCTATATGGATGCCTTATTAAAAGATAGTGAACATCTGGCGGCTATGATAGATAATGTGTCTTCTGTTGATAATTTGGATTTTATTATGGAGAGTGATGCGCTGGGGCATACAGTAATGGATCAGAGGCAAGGACACGAGAGTTTGGTTGCTGTTGCTGGAACAGTTACTCTTGAAGAGGTCAACTCTATTGGCGCCAAGGTGTTGGAATTTGTTTCTAATTTTGGAAATCCTACTGCACCTCTTCCTGCAGCGATTGTTGCTTGTGTTCCAAAGAGAGTGCATGTAGAGGGAATAGGTGAAACTGAGTTCACCATTTCCTCTAATGAGATTATAGCTGCTACAAAAGCTGGATTGGAGGAACCCATTGAACCTGAGCCAGAGCTTGAGGTGCCAAAAGAATTGATATCTTCATTGCAGTTACAGGAATTAAGGCAGCGGCGCATGCCATCCTTCATTCCCTGTAGTCCAGAAATAAATGTGACTAAAGTTTATGACAAAGAAACGGGGATCACTAAATGTCGTCTGTCAAATGGAATTTCTGTAAATTACAAGATTTCAAAAAGTGAGGCAAGGGGAGGCGTAATGCGGCTTATAGTTGGCGGTGGACGGGCAGCTGAAAGTTCTGAGTCCAGAGGTTCTGTTGTTGTGGGTGTACGAACTCTTAGTGAGGGAGGTCGTGTTGGCAACTTCTCAAGGGAGCAGGTAGAACTGTTCTGTATGAATCACCTGATTAACTGCTCATTGGAGTCCACTGAGGAATTTATTTCTATGGAATTCCGTTTTACTTTAAGAGATAATGGAATGCGTGCGGCTTTCCAGTTGCTTCATATGGTACTTGAGCATAGTGTCTGGCTGGATGATGCATTTGATAGAGCAAGGCAATTGTATTTGTCATATTATCAGTCCATTCCCAAAAGCTTGGAGCGCTCTACTGCTCACAAACTCATGTTAGCAATGATGGACGGAGACGAGCGGTTTGTAGAGCCTACACCAAAGTCATTGCAAAATTTGACATTGCAATCTGTAAAGGATGCAGTGATGAACCAATTTGTAGGCAGTAACATGGAGGTAAGTATTGTTGGGGACTTCTCAGAGGAGGATGTTGAGTCTTGTATTCTTGATTACTTGGGAACCGTAAGATCGAAAGGAAATTATGAGATGGAGAATGCTTATAATCCTATTGTATTCCGATCATCTCCTTCTGATTTGCAGTCTCAGCAAGTATTCTTGAAGGATACGGATGAGAGAGCATGTGCATATATTGCAGGCCCTGCACCAAACCGATGGGGTTTTACAGTCGATGGCAAAGATCTGTTTGAGTCATTTAGTCATATTTCAACTCACGATGATGCAAAACTGAAATCGGAGGAGGTGCTTATGGTGGATGAGGATACTCAGAAGGATGTGCAAAGAAAACTCTGTGGTCATCCCCTGTTCTTTGGCATCACAATGGGGCTCCTGGCTGAGATTATAAATTCTAGGCTTTTTACGACTGTCAGAGACTCTCTTGGATTGACATATGATGTATCATTCGAGTTGAACCTTTTTGATAGGCTGAATCTTGGATGGTATGTCATATCTGTAACATCAACCCCTGCGAAGGTTCACAAAGCTGTGGATGCATGCAAGAATGTCCTTAGAGGTTTACATGGCAACAAGATTTGCCAAAGGGAGTTGGACAGGGCAAAGCGGACCCTTCTGATGAGGCATGAAGCTGAGATCAAGTCAAATGCGTATTGGCTTGGACTGTTGGCTCATTTGCAGGCTTCTTCTGTTCCAAGGAAGGACATATCGTGCATCAAAGATCTCACAAATTTATATGAGGTTGCTTCTATTGATGACATATACCTTGCATATGATCAATTGAAAGTAGGCGATGATTCTTTGTACTCGTGCATTGGAGTTGCTGGAGCTCAAGCCGGAGATGAAATAACTGTCGAAGAATCAGTTGATGGCTTTCCAGGAATCTTCCCTGTTGGGCGTGGTTTATCAACTATGACACGGCCTACAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0007275 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M16 Peptidase_M16_C

Protein Analysis

1257

Amino Acids

140.49

Weight (kDa)

6.25

Isoelectric Point (pI)

40.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	212 - 347	4.8e-21	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	366 - 590	1.9e-12	Peptidase M16 inactive domain
Peptidase_M16_C	PF05193	921 - 1151	4.7e-23	Peptidase M16 inactive domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003778)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42390 AT5G42390
fragaria_vesca	FvH4_1g30291 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800 FvH4_7g03800
malus_domestica	MD00G1159400.v1.1 MD00G1204700.v1.1
prunus_persica	Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1 Prupe.7G271900_v2.0.a1
pyrus_communis	pycom290g00010
rosa_chinensis	RchiOBHm_Chr3g0491051
rosa_laevigata	RLG00000022866
rosa_multiflora	Rmu_co8044182.1_g000001 Rmu_ssc0000386.1_g000043
rosa_roxburghii	Rroxscaffold_5G00351210 Rroxscaffold_6G00393270
rosa_rugosa	Rorug03G0245500 Rorug04G0069600 Rorug04G0069700 Rorug04G0070700 Rorug04G0070800
rosa_samantha	Rh3AG295700 Rh3BG331900 Rh3CG329000 Rh3DG329500
rosa_wichuraiana	Rw3G026110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2928, 3227
AarI	CACCTGC	1 cut(s)	1264
Acc36I	ACCTGC	3 cut(s)	191, 1264, 2446
AccB1I	GGYRCC	2 cut(s)	1946, 2143
AccB7I	CCANNNNNTGG	1 cut(s)	1674
AccBSI	CCGCTC	1 cut(s)	2728
AccI	GTMKAC	1 cut(s)	184
AclWI	GGATC	4 cut(s)	703, 1614, 1881, 2270
AcoI	YGGCCR	1 cut(s)	6
AcyI	GRCGYC	2 cut(s)	181, 1947
AfaI	GTAC	9 cut(s)	216, 484, 894, 1136, 1636, 1701, 2409, 2589, 3644
AfeI	AGCGCT	1 cut(s)	2681
AfiI	CCNNNNNNNGG	3 cut(s)	410, 1674, 3355
AflIII	ACRYGT	1 cut(s)	745
AjnI	CCWGG	7 cut(s)	176, 289, 488, 535, 1411, 3213, 3715
AjuI	GAANNNNNNNTTGG	2 cut(s)	1200, 1232
Alw21I	GWGCWC	2 cut(s)	124, 3670
Alw26I	GTCTC	5 cut(s)	60, 256, 2715, 2973, 3249
AlwI	GGATC	4 cut(s)	703, 1614, 1881, 2270
AlwNI	CAGNNNCTG	4 cut(s)	275, 520, 1250, 3026
Aor51HI	AGCGCT	1 cut(s)	2681
AoxI	GGCC	6 cut(s)	6, 127, 457, 1228, 3022, 3758
ApaI	GGGCCC	1 cut(s)	461
ApeKI	GCWGC	6 cut(s)	476, 625, 2003, 2090, 2188, 2366
ArsI	GACNNNNNNTTYG	4 cut(s)	319, 351, 2714, 2746
AseI	ATTAAT	2 cut(s)	231, 1557
Asp700I	GAANNNNTTC	2 cut(s)	1327, 1461
AspLEI	GCGC	5 cut(s)	630, 1855, 1949, 2196, 2682
AspS9I	GGNCC	5 cut(s)	128, 457, 458, 3023, 3447
AsuHPI	GGTGA	8 cut(s)	127, 887, 914, 1511, 1754, 2056, 2063, 2471
AsuII	TTCGAA	1 cut(s)	83
AvaII	GGWCC	1 cut(s)	3447
BaeGI	GKGCMC	2 cut(s)	461, 1299
BaeI	ACNNNNGTAYC	2 cut(s)	1558, 1591
BanI	GGYRCC	2 cut(s)	1946, 2143
BanII	GRGCYC	4 cut(s)	461, 799, 3213, 3670
BauI	CACGAG	4 cut(s)	123, 942, 1889, 3646
BbsI	GAAGAC	3 cut(s)	494, 1376, 1806
Bbv12I	GWGCWC	2 cut(s)	124, 3670
BbvI	GCAGC	6 cut(s)	488, 612, 2015, 2077, 2200, 2378
BceAI	ACGGC	1 cut(s)	974
BcgI	CGANNNNNNTGC	6 cut(s)	134, 168, 996, 1030, 1361, 1395
BciT130I	CCWGG	7 cut(s)	178, 291, 490, 537, 1413, 3215, 3717
BciVI	GTATCC	2 cut(s)	2983, 3139
BclI	TGATCA	2 cut(s)	1342, 3613
BcoDI	GTCTC	5 cut(s)	60, 256, 2715, 2973, 3249
BfaI	CTAG	5 cut(s)	312, 1232, 1263, 1284, 3234
BfmI	CTRYAG	4 cut(s)	346, 996, 2001, 2215
BfoI	RGCGCY	2 cut(s)	1950, 2683
BfuAI	ACCTGC	3 cut(s)	191, 1264, 2446
BfuI	GTATCC	2 cut(s)	2983, 3139
BglII	AGATCT	2 cut(s)	3064, 3559
BlpI	GCTNAGC	1 cut(s)	1052
Bme1390I	CCNGG	7 cut(s)	178, 291, 490, 537, 1413, 3215, 3717
Bme18I	GGWCC	1 cut(s)	3447
BmgT120I	GGNCC	5 cut(s)	128, 457, 458, 3023, 3447
BmiI	GGNNCC	8 cut(s)	459, 1064, 1948, 2115, 2145, 2887, 3212, 3449
BmrFI	CCNGG	7 cut(s)	178, 291, 490, 537, 1413, 3215, 3717
BpiI	GAAGAC	3 cut(s)	494, 1376, 1806
BplI	GAGNNNNNCTC	2 cut(s)	1922, 1954
BpmI	CTGGAG	3 cut(s)	527, 558, 3684
Bpu10I	CCTNAGC	1 cut(s)	2127
Bpu1102I	GCTNAGC	1 cut(s)	1052
Bpu14I	TTCGAA	1 cut(s)	83
BpuEI	CTTGAG	5 cut(s)	1703, 2159, 2431, 2612, 3654
BsaBI	GATNNNNATC	1 cut(s)	876
BsaHI	GRCGYC	2 cut(s)	181, 1947
BsaJI	CCNNGG	5 cut(s)	362, 453, 757, 1950, 3533
BsaXI	ACNNNNNCTCC	4 cut(s)	255, 285, 2418, 2448
Bsc4I	CCNNNNNNNGG	3 cut(s)	410, 1674, 3355
Bse1I	ACTGG	6 cut(s)	269, 900, 1018, 1231, 1324, 2572
Bse3DI	GCAATG	5 cut(s)	1284, 2165, 2715, 2753, 2768
Bse8I	GATNNNNATC	1 cut(s)	876
BseBI	CCWGG	7 cut(s)	178, 291, 490, 537, 1413, 3215, 3717
BseDI	CCNNGG	5 cut(s)	362, 453, 757, 1950, 3533
BseJI	GATNNNNATC	1 cut(s)	876
BseLI	CCNNNNNNNGG	3 cut(s)	410, 1674, 3355
BseMI	GCAATG	5 cut(s)	1284, 2165, 2715, 2753, 2768
BseMII	CTCAG	9 cut(s)	2049, 2118, 2370, 2499, 2859, 2984, 3164, 3210, 3462
BseNI	ACTGG	6 cut(s)	269, 900, 1018, 1231, 1324, 2572
BseRI	GAGGAG	2 cut(s)	2864, 3137
BseSI	GKGCMC	2 cut(s)	461, 1299
BseXI	GCAGC	6 cut(s)	488, 612, 2015, 2077, 2200, 2378
BseYI	CCCAGC	1 cut(s)	1855
BsgI	GTGCAG	4 cut(s)	219, 1236, 1974, 3012
BshFI	GGCC	6 cut(s)	8, 129, 459, 1230, 3024, 3760
BshNI	GGYRCC	2 cut(s)	1946, 2143
BsiHKAI	GWGCWC	2 cut(s)	124, 3670
BsiSI	CCGG	1 cut(s)	3675
BslFI	GGGAC	5 cut(s)	17, 34, 802, 1184, 2852
BslI	CCNNNNNNNGG	3 cut(s)	410, 1674, 3355
BsmAI	GTCTC	5 cut(s)	60, 256, 2715, 2973, 3249
BsmBI	CGTCTC	2 cut(s)	60, 2715
BsmFI	GGGAC	5 cut(s)	17, 34, 802, 1184, 2852
BsmI	GAATGC	3 cut(s)	1036, 2562, 2926
BsnI	GGCC	6 cut(s)	8, 129, 459, 1230, 3024, 3760
Bsp119I	TTCGAA	1 cut(s)	83
Bsp120I	GGGCCC	1 cut(s)	457
Bsp1286I	GDGCHC	6 cut(s)	124, 461, 799, 1299, 3213, 3670
Bsp1407I	TGTACA	2 cut(s)	214, 1699
Bsp1720I	GCTNAGC	1 cut(s)	1052
BspANI	GGCC	6 cut(s)	8, 129, 459, 1230, 3024, 3760
BspCNI	CTCAG	9 cut(s)	2050, 2119, 2371, 2500, 2858, 2983, 3163, 3211, 3463
BspLI	GGNNCC	8 cut(s)	459, 1064, 1948, 2115, 2145, 2887, 3212, 3449
BspMAI	CTGCAG	1 cut(s)	2005
BspMI	ACCTGC	3 cut(s)	191, 1264, 2446
BspPI	GGATC	4 cut(s)	703, 1614, 1881, 2270
BspQI	GCTCTTC	1 cut(s)	1074
BspT104I	TTCGAA	1 cut(s)	83
BspT107I	GGYRCC	2 cut(s)	1946, 2143
BsrBI	CCGCTC	1 cut(s)	2728
BsrDI	GCAATG	5 cut(s)	1284, 2165, 2715, 2753, 2768
BsrGI	TGTACA	2 cut(s)	214, 1699
BsrI	ACTGG	6 cut(s)	269, 900, 1018, 1231, 1324, 2572
BssECI	CCNNGG	5 cut(s)	362, 453, 757, 1950, 3533
BssNI	GRCGYC	2 cut(s)	181, 1947
BssSI	CACGAG	4 cut(s)	123, 942, 1889, 3646
BssT1I	CCWWGG	4 cut(s)	362, 757, 1950, 3533
Bst2BI	CACGAG	4 cut(s)	123, 942, 1889, 3646
Bst2UI	CCWGG	7 cut(s)	178, 291, 490, 537, 1413, 3215, 3717
Bst6I	CTCTTC	4 cut(s)	1074, 1517, 1923, 2001
BstACI	GRCGYC	2 cut(s)	181, 1947
BstAPI	GCANNNNNTGC	2 cut(s)	3017, 3026
BstAUI	TGTACA	2 cut(s)	214, 1699
BstBI	TTCGAA	1 cut(s)	83
BstC8I	GCNNGC	7 cut(s)	424, 963, 2198, 2562, 3022, 3379, 3519
BstDSI	CCRYGG	1 cut(s)	453
BstH2I	RGCGCY	2 cut(s)	1950, 2683
BstHHI	GCGC	5 cut(s)	630, 1855, 1949, 2196, 2682
BstMAI	GTCTC	5 cut(s)	60, 256, 2715, 2973, 3249
BstNI	CCWGG	7 cut(s)	178, 291, 490, 537, 1413, 3215, 3717
BstNSI	RCATGY	8 cut(s)	434, 476, 749, 965, 2039, 2200, 3009, 3381
BstSCI	CCNGG	7 cut(s)	176, 289, 488, 535, 1411, 3213, 3715
BstSFI	CTRYAG	4 cut(s)	346, 996, 2001, 2215
BstSLI	GKGCMC	2 cut(s)	461, 1299
BstV1I	GCAGC	6 cut(s)	488, 612, 2015, 2077, 2200, 2378
BstV2I	GAAGAC	3 cut(s)	494, 1376, 1806
BstX2I	RGATCY	2 cut(s)	3064, 3559
BstXI	CCANNNNNNTGG	3 cut(s)	1957, 2674, 3039
BstYI	RGATCY	2 cut(s)	3064, 3559
BsuI	GTATCC	2 cut(s)	2983, 3139
BsuRI	GGCC	6 cut(s)	8, 129, 459, 1230, 3024, 3760
BtgI	CCRYGG	1 cut(s)	453
BtgZI	GCGATG	1 cut(s)	3645
BtsI	GCAGTG	2 cut(s)	124, 2796
BtsIMutI	CAGTG	7 cut(s)	124, 276, 343, 813, 837, 2505, 2796
BveI	ACCTGC	3 cut(s)	191, 1264, 2446
Cac8I	GCNNGC	7 cut(s)	424, 963, 2198, 2562, 3022, 3379, 3519
CaiI	CAGNNNCTG	4 cut(s)	275, 520, 1250, 3026
CfoI	GCGC	5 cut(s)	630, 1855, 1949, 2196, 2682
Cfr13I	GGNCC	5 cut(s)	128, 457, 458, 3023, 3447
CseI	GACGC	1 cut(s)	170
Csp6I	GTAC	9 cut(s)	215, 483, 893, 1135, 1635, 1700, 2408, 2588, 3643
CviQI	GTAC	9 cut(s)	215, 483, 893, 1135, 1635, 1700, 2408, 2588, 3643
DinI	GGCGCC	1 cut(s)	1948
DraI	TTTAAA	1 cut(s)	1360
EaeI	YGGCCR	1 cut(s)	6
Eam1104I	CTCTTC	4 cut(s)	1074, 1517, 1923, 2001
EarI	CTCTTC	4 cut(s)	1074, 1517, 1923, 2001
EciI	GGCGGA	1 cut(s)	645
Ecl136II	GAGCTC	1 cut(s)	3668
Eco130I	CCWWGG	4 cut(s)	362, 757, 1950, 3533
Eco24I	GRGCYC	4 cut(s)	461, 799, 3213, 3670
Eco32I	GATATC	1 cut(s)	2160
Eco47I	GGWCC	1 cut(s)	3447
Eco47III	AGCGCT	1 cut(s)	2681
Eco53kI	GAGCTC	1 cut(s)	3668
EcoICRI	GAGCTC	1 cut(s)	3668
EcoO109I	RGGNCCY	1 cut(s)	3023
EcoRI	GAATTC	2 cut(s)	97, 2528
EcoRII	CCWGG	7 cut(s)	176, 289, 488, 535, 1411, 3213, 3715
EcoRV	GATATC	1 cut(s)	2160
EcoT14I	CCWWGG	4 cut(s)	362, 757, 1950, 3533
EcoT22I	ATGCAT	2 cut(s)	2620, 3379
EcoT38I	GRGCYC	4 cut(s)	461, 799, 3213, 3670
EgeI	GGCGCC	1 cut(s)	1948
EheI	GGCGCC	1 cut(s)	1948
ErhI	CCWWGG	4 cut(s)	362, 757, 1950, 3533
Esp3I	CGTCTC	2 cut(s)	60, 2715
FalI	AAGNNNNNCTT	2 cut(s)	486, 518
FaqI	GGGAC	5 cut(s)	17, 34, 802, 1184, 2852
FauNDI	CATATG	7 cut(s)	445, 735, 889, 1495, 2583, 3274, 3610
FbaI	TGATCA	2 cut(s)	1342, 3613
FblI	GTMKAC	1 cut(s)	184
FriOI	GRGCYC	4 cut(s)	461, 799, 3213, 3670
FspBI	CTAG	5 cut(s)	312, 1232, 1263, 1284, 3234
GlaI	GCGC	5 cut(s)	629, 1854, 1948, 2195, 2681
GsaI	CCCAGC	1 cut(s)	1859
GsuI	CTGGAG	3 cut(s)	527, 558, 3684
HaeII	RGCGCY	2 cut(s)	1950, 2683
HaeIII	GGCC	6 cut(s)	8, 129, 459, 1230, 3024, 3760
HapII	CCGG	1 cut(s)	3675
HgaI	GACGC	1 cut(s)	170
HhaI	GCGC	5 cut(s)	630, 1855, 1949, 2196, 2682
Hin1I	GRCGYC	2 cut(s)	181, 1947
Hin6I	GCGC	5 cut(s)	628, 1853, 1947, 2194, 2680
HinP1I	GCGC	5 cut(s)	628, 1853, 1947, 2194, 2680
HincII	GTYRAC	2 cut(s)	1012, 1936
HindII	GTYRAC	2 cut(s)	1012, 1936
HindIII	AAGCTT	3 cut(s)	1049, 1301, 2671
HpaII	CCGG	1 cut(s)	3675
HphI	GGTGA	8 cut(s)	127, 887, 914, 1511, 1754, 2056, 2063, 2471
Hpy99I	CGWCG	1 cut(s)	74
HpyCH4IV	ACGT	2 cut(s)	30, 772
HpySE526I	ACGT	2 cut(s)	30, 772
Hsp92I	GRCGYC	2 cut(s)	181, 1947
HspAI	GCGC	5 cut(s)	628, 1853, 1947, 2194, 2680
KasI	GGCGCC	1 cut(s)	1946
Ksp22I	TGATCA	2 cut(s)	1342, 3613
LguI	GCTCTTC	1 cut(s)	1074
LmnI	GCTCC	6 cut(s)	517, 1264, 2452, 2677, 3216, 3665
Lsp1109I	GCAGC	6 cut(s)	488, 612, 2015, 2077, 2200, 2378
MaeI	CTAG	5 cut(s)	312, 1232, 1263, 1284, 3234
MaeII	ACGT	2 cut(s)	30, 772
MbiI	CCGCTC	1 cut(s)	2728
MfeI	CAATTG	4 cut(s)	699, 1596, 2636, 3617
MflI	RGATCY	2 cut(s)	3064, 3559
MhlI	GDGCHC	6 cut(s)	124, 461, 799, 1299, 3213, 3670
Mly113I	GGCGCC	1 cut(s)	1947
MlyI	GAGTC	6 cut(s)	1610, 2390, 2510, 2870, 3083, 3251
MmeI	TCCRAC	3 cut(s)	197, 1938, 3411
Mph1103I	ATGCAT	2 cut(s)	2620, 3379
MroXI	GAANNNNTTC	2 cut(s)	1327, 1461
MslI	CAYNNNNRTG	3 cut(s)	435, 3102, 3203
MspA1I	CMGCKG	2 cut(s)	2191, 2369
MspI	CCGG	1 cut(s)	3675
MspR9I	CCNGG	7 cut(s)	178, 291, 490, 537, 1413, 3215, 3717
MunI	CAATTG	4 cut(s)	699, 1596, 2636, 3617
Mva1269I	GAATGC	3 cut(s)	1036, 2562, 2926
MvaI	CCWGG	7 cut(s)	178, 291, 490, 537, 1413, 3215, 3717
NarI	GGCGCC	1 cut(s)	1947
NdeI	CATATG	7 cut(s)	445, 735, 889, 1495, 2583, 3274, 3610
NlaIV	GGNNCC	8 cut(s)	459, 1064, 1948, 2115, 2145, 2887, 3212, 3449
NmuCI	GTSAC	6 cut(s)	271, 1404, 1499, 1613, 1639, 2233
NsiI	ATGCAT	2 cut(s)	2620, 3379
NspI	RCATGY	8 cut(s)	434, 476, 749, 965, 2039, 2200, 3009, 3381
NspV	TTCGAA	1 cut(s)	83
PaeI	GCATGC	3 cut(s)	965, 2200, 3381
PaqCI	CACCTGC	1 cut(s)	1264
PciI	ACATGT	1 cut(s)	745
PciSI	GCTCTTC	1 cut(s)	1074
PcsI	WCGNNNNNNNCGW	1 cut(s)	121
PctI	GAATGC	3 cut(s)	1036, 2562, 2926
PdmI	GAANNNNTTC	2 cut(s)	1327, 1461
PfeI	GAWTC	8 cut(s)	866, 967, 1462, 2476, 3313, 3635, 3698, 3720
PflMI	CCANNNNNTGG	1 cut(s)	1674
PfoI	TCCNGGA	4 cut(s)	289, 535, 1411, 3715
PleI	GAGTC	6 cut(s)	1610, 2389, 2509, 2869, 3082, 3251
PluTI	GGCGCC	1 cut(s)	1950
PpsI	GAGTC	6 cut(s)	1610, 2389, 2509, 2869, 3082, 3251
PscI	ACATGT	1 cut(s)	745
PshBI	ATTAAT	2 cut(s)	231, 1557
PsiI	TTATAA	2 cut(s)	2928, 3227
Psp124BI	GAGCTC	1 cut(s)	3670
Psp6I	CCWGG	7 cut(s)	176, 289, 488, 535, 1411, 3213, 3715
PspFI	CCCAGC	1 cut(s)	1855
PspGI	CCWGG	7 cut(s)	176, 289, 488, 535, 1411, 3213, 3715
PspN4I	GGNNCC	8 cut(s)	459, 1064, 1948, 2115, 2145, 2887, 3212, 3449
PspOMI	GGGCCC	1 cut(s)	457
PspPI	GGNCC	5 cut(s)	128, 457, 458, 3023, 3447
PstI	CTGCAG	1 cut(s)	2005
PstNI	CAGNNNCTG	4 cut(s)	275, 520, 1250, 3026
PsuI	RGATCY	2 cut(s)	3064, 3559
PvuII	CAGCTG	1 cut(s)	2369
RsaI	GTAC	9 cut(s)	216, 484, 894, 1136, 1636, 1701, 2409, 2589, 3644
RsaNI	GTAC	9 cut(s)	215, 483, 893, 1135, 1635, 1700, 2408, 2588, 3643
RseI	CAYNNNNRTG	3 cut(s)	435, 3102, 3203
SacI	GAGCTC	1 cut(s)	3670
SapI	GCTCTTC	1 cut(s)	1074
Sau96I	GGNCC	5 cut(s)	128, 457, 458, 3023, 3447
SchI	GAGTC	6 cut(s)	1610, 2390, 2510, 2870, 3083, 3251
ScrFI	CCNGG	7 cut(s)	178, 291, 490, 537, 1413, 3215, 3717
SduI	GDGCHC	6 cut(s)	124, 461, 799, 1299, 3213, 3670
SfcI	CTRYAG	4 cut(s)	346, 996, 2001, 2215
SfoI	GGCGCC	1 cut(s)	1948
SfuI	TTCGAA	1 cut(s)	83
SinI	GGWCC	1 cut(s)	3447
SmiMI	CAYNNNNRTG	3 cut(s)	435, 3102, 3203
SmlI	CTYRAG	5 cut(s)	1718, 2138, 2446, 2591, 3669
SmoI	CTYRAG	5 cut(s)	1718, 2138, 2446, 2591, 3669
SphI	GCATGC	3 cut(s)	965, 2200, 3381
SspDI	GGCGCC	1 cut(s)	1946
SspI	AATATT	1 cut(s)	1530
SspMI	CTAG	5 cut(s)	312, 1232, 1263, 1284, 3234
SstI	GAGCTC	1 cut(s)	3670
StyD4I	CCNGG	7 cut(s)	176, 289, 488, 535, 1411, 3213, 3715
StyI	CCWWGG	4 cut(s)	362, 757, 1950, 3533
TaiI	ACGT	2 cut(s)	33, 775
TaqI	TCGA	8 cut(s)	83, 604, 799, 1006, 2897, 3054, 3288, 3693
TatI	WGTACW	6 cut(s)	214, 482, 892, 1634, 1699, 3642
TauI	GCSGC	6 cut(s)	8, 94, 1796, 2194, 2344, 2567
TfiI	GAWTC	8 cut(s)	866, 967, 1462, 2476, 3313, 3635, 3698, 3720
TscAI	CASTG	7 cut(s)	124, 276, 350, 820, 837, 2512, 2796
TseFI	GTSAC	6 cut(s)	271, 1404, 1499, 1613, 1639, 2233
TseI	GCWGC	6 cut(s)	476, 625, 2003, 2090, 2188, 2366
Tsp45I	GTSAC	6 cut(s)	271, 1404, 1499, 1613, 1639, 2233
TspGWI	ACGGA	6 cut(s)	32, 36, 1327, 2522, 2733, 3008
TspRI	CASTG	7 cut(s)	124, 276, 350, 820, 837, 2512, 2796
Van91I	CCANNNNNTGG	1 cut(s)	1674
VpaK11BI	GGWCC	1 cut(s)	3447
VspI	ATTAAT	2 cut(s)	231, 1557
XceI	RCATGY	8 cut(s)	434, 476, 749, 965, 2039, 2200, 3009, 3381
XmiI	GTMKAC	1 cut(s)	184
XmnI	GAANNNNTTC	2 cut(s)	1327, 1461
XspI	CTAG	5 cut(s)	312, 1232, 1263, 1284, 3234
Zsp2I	ATGCAT	2 cut(s)	2620, 3379

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.