AT5G43910

Belongs to the carbohydrate kinase PfkB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004679
Physical Location & Seq
Reverse (-)
17667310 .. 17670292
2983 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G43910.3

Sequence Viewer

Length: 858 bp
ATGTCATCTTGCTCCGACGAAGCGATTCCCGGCCAACCAATCGTCCTCGGTTGTGGTCAACTTTGTCTAGATTACTTAGTCACAGTGGCGTCTTTTCCAATTCCCGATCAAAAGATCCGAGGCACAAGCTTCAAGGTCCAAGGAGGTGGTAACACTGGGAATGCTTTAACATGTGTTGCTCGTTTGGGTTTGCCTTGTCGAATCTTGGCTAAGGTTGCTGATGATTCTCACGGGCGATATATGGTAGAAGAACTCGAATCTAGCGGTGTGGATACTTCGTTTTGTATGAGTGCTAAAGATGGAGCTTCACATTTTAATTACGTCATTGTAGATAACCAAACGAATACTCGTACTTGTATTTACACTCCAGGATATCCTCCTTTGCTACCAGATGACCTTACTGAATCTCTACTTCTAGATGTTCTTGATGGAGTAAGAGTTCTATATGTAAATGGAAGGTCCCGTGAAGCCGAATTGCTTCTTGCGCAAAAGGCACATAGCAAGAATATACCAATCTTAATTAATGCAGAGAAAAAAAGGACAGGGCTAGATGAGCTCATTGACTTAGCTGATTATGCTATTTGTTCTACTAACTTCCCTCAGGAATGGACAGGGGCACCCTCATCTCCCAGTGCACTTCTCTCTATGCTCATAAGATTACCAAAGCTAAAATTTGTGATCATGACTTTGGGGGAACACGGTTGTGTGATGCTTGAGAGATGTTCCAGTGAGGTTTCAGGGTCAGAAGAAGAGACAGACATAGATGAATTACACGAGTCTCTGAAACAAAGCACAGACTTTACAAGTGTCTTACCGGTCTGCAATTCATCGGGAATGTTACAGGGAGATTGGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

285

Amino Acids

30.89

Weight (kDa)

4.69

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 22 - 240 3e-20 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 486
AccB1I GGYRCC 1 cut(s) 616
AciI CCGC 1 cut(s) 264
AclWI GGATC 1 cut(s) 109
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 1 cut(s) 671
AcyI GRCGYC 1 cut(s) 89
AfaI GTAC 1 cut(s) 352
AflIII ACRYGT 1 cut(s) 170
AgeI ACCGGT 1 cut(s) 814
AgsI TTSAA 1 cut(s) 133
AjnI CCWGG 1 cut(s) 367
AleI CACNNNNGTG 1 cut(s) 702
AloI GAACNNNNNNTCC 2 cut(s) 423, 455
AluBI AGCT 5 cut(s) 129, 305, 556, 569, 667
AluI AGCT 5 cut(s) 129, 305, 556, 569, 667
Alw21I GWGCWC 2 cut(s) 558, 637
Alw26I GTCTC 2 cut(s) 746, 783
Alw44I GTGCAC 1 cut(s) 633
AlwI GGATC 1 cut(s) 109
AoxI GGCC 1 cut(s) 31
ApaLI GTGCAC 1 cut(s) 633
ApoI RAATTY 1 cut(s) 671
AseI ATTAAT 1 cut(s) 522
AsiGI ACCGGT 1 cut(s) 814
Asp700I GAANNNNTTC 2 cut(s) 24, 477
AspLEI GCGC 1 cut(s) 487
AspS9I GGNCC 2 cut(s) 136, 459
AsuC2I CCSGG 1 cut(s) 30
AvaII GGWCC 2 cut(s) 136, 459
AxyI CCTNAGG 1 cut(s) 600
BaeGI GKGCMC 2 cut(s) 619, 637
BanI GGYRCC 1 cut(s) 616
BanII GRGCYC 1 cut(s) 558
BauI CACGAG 1 cut(s) 773
Bbv12I GWGCWC 2 cut(s) 558, 637
BccI CCATC 2 cut(s) 293, 422
BciT130I CCWGG 1 cut(s) 369
BciVI GTATCC 1 cut(s) 265
BclI TGATCA 1 cut(s) 678
BcnI CCSGG 1 cut(s) 30
BcoDI GTCTC 2 cut(s) 746, 783
BfaI CTAG 4 cut(s) 68, 261, 416, 548
BfuI GTATCC 1 cut(s) 265
Bme1390I CCNGG 2 cut(s) 30, 369
Bme18I GGWCC 2 cut(s) 136, 459
BmgT120I GGNCC 2 cut(s) 136, 459
BmiI GGNNCC 2 cut(s) 461, 618
BmrFI CCNGG 2 cut(s) 30, 369
BmrI ACTGGG 2 cut(s) 165, 624
BmsI GCATC 1 cut(s) 699
BmuI ACTGGG 2 cut(s) 165, 624
BpmI CTGGAG 1 cut(s) 351
Bpu10I CCTNAGC 1 cut(s) 210
BpuEI CTTGAG 1 cut(s) 734
BpuMI CCSGG 1 cut(s) 30
BsaHI GRCGYC 1 cut(s) 89
BsaJI CCNNGG 3 cut(s) 46, 118, 139
BsaWI WCCGGW 1 cut(s) 814
BsaXI ACNNNNNCTCC 2 cut(s) 423, 453
Bse118I RCCGGY 1 cut(s) 814
Bse1I ACTGG 3 cut(s) 160, 630, 726
Bse21I CCTNAGG 1 cut(s) 600
BseBI CCWGG 1 cut(s) 369
BseDI CCNNGG 3 cut(s) 46, 118, 139
BseMII CTCAG 1 cut(s) 614
BseNI ACTGG 3 cut(s) 160, 630, 726
BseSI GKGCMC 2 cut(s) 619, 637
BshFI GGCC 1 cut(s) 33
BshNI GGYRCC 1 cut(s) 616
BshTI ACCGGT 1 cut(s) 814
BsiHKAI GWGCWC 2 cut(s) 558, 637
BsiSI CCGG 2 cut(s) 30, 815
BslFI GGGAC 1 cut(s) 445
BsmAI GTCTC 2 cut(s) 746, 783
BsmFI GGGAC 1 cut(s) 445
BsmI GAATGC 1 cut(s) 166
BsnI GGCC 1 cut(s) 33
Bsp1286I GDGCHC 3 cut(s) 558, 619, 637
Bsp143I GATC 3 cut(s) 106, 114, 678
BspACI CCGC 1 cut(s) 264
BspANI GGCC 1 cut(s) 33
BspCNI CTCAG 1 cut(s) 613
BspHI TCATGA 1 cut(s) 681
BspLI GGNNCC 2 cut(s) 461, 618
BspPI GGATC 1 cut(s) 109
BspT107I GGYRCC 1 cut(s) 616
BsrFI RCCGGY 1 cut(s) 814
BsrI ACTGG 3 cut(s) 160, 630, 726
BssAI RCCGGY 1 cut(s) 814
BssECI CCNNGG 3 cut(s) 46, 118, 139
BssMI GATC 3 cut(s) 106, 114, 678
BssNI GRCGYC 1 cut(s) 89
BssSI CACGAG 1 cut(s) 773
BssT1I CCWWGG 1 cut(s) 139
Bst2BI CACGAG 1 cut(s) 773
Bst2UI CCWGG 1 cut(s) 369
Bst4CI ACNGT 2 cut(s) 85, 701
Bst6I CTCTTC 1 cut(s) 744
BstACI GRCGYC 1 cut(s) 89
BstDEI CTNAG 4 cut(s) 76, 210, 565, 600
BstHHI GCGC 1 cut(s) 487
BstKTI GATC 3 cut(s) 109, 117, 681
BstMAI GTCTC 2 cut(s) 746, 783
BstMBI GATC 3 cut(s) 106, 114, 678
BstMWI GCNNNNNNNGC 5 cut(s) 215, 484, 491, 553, 575
BstNI CCWGG 1 cut(s) 369
BstNSI RCATGY 1 cut(s) 174
BstSCI CCNGG 2 cut(s) 28, 367
BstSLI GKGCMC 2 cut(s) 619, 637
BstX2I RGATCY 1 cut(s) 114
BstXI CCANNNNNNTGG 1 cut(s) 146
BstYI RGATCY 1 cut(s) 114
Bsu36I CCTNAGG 1 cut(s) 600
BsuI GTATCC 1 cut(s) 265
BsuRI GGCC 1 cut(s) 33
BtsIMutI CAGTG 4 cut(s) 90, 153, 637, 733
CciI TCATGA 1 cut(s) 681
CfoI GCGC 1 cut(s) 487
Cfr10I RCCGGY 1 cut(s) 814
Cfr13I GGNCC 2 cut(s) 136, 459
CseI GACGC 1 cut(s) 78
Csp6I GTAC 1 cut(s) 351
CspAI ACCGGT 1 cut(s) 814
CviAII CATG 2 cut(s) 171, 682
CviJI RGCY 9 cut(s) 33, 129, 209, 305, 470, 547, 556, 569, 667
CviKI_1 RGCY 9 cut(s) 33, 129, 209, 305, 470, 547, 556, 569, 667
CviQI GTAC 1 cut(s) 351
DdeI CTNAG 4 cut(s) 76, 210, 565, 600
DpnI GATC 3 cut(s) 108, 116, 680
DpnII GATC 3 cut(s) 106, 114, 678
EaeI YGGCCR 1 cut(s) 31
Eam1104I CTCTTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 744
Ecl136II GAGCTC 1 cut(s) 556
Eco130I CCWWGG 1 cut(s) 139
Eco24I GRGCYC 1 cut(s) 558
Eco32I GATATC 1 cut(s) 374
Eco47I GGWCC 2 cut(s) 136, 459
Eco53kI GAGCTC 1 cut(s) 556
Eco81I CCTNAGG 1 cut(s) 600
EcoICRI GAGCTC 1 cut(s) 556
EcoO109I RGGNCCY 1 cut(s) 459
EcoRII CCWGG 1 cut(s) 367
EcoRV GATATC 1 cut(s) 374
EcoT14I CCWWGG 1 cut(s) 139
EcoT38I GRGCYC 1 cut(s) 558
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 2 cut(s) 174, 685
FaqI GGGAC 1 cut(s) 445
FatI CATG 2 cut(s) 170, 681
FbaI TGATCA 1 cut(s) 678
FriOI GRGCYC 1 cut(s) 558
FspBI CTAG 4 cut(s) 68, 261, 416, 548
FspI TGCGCA 1 cut(s) 486
GlaI GCGC 1 cut(s) 486
GsuI CTGGAG 1 cut(s) 351
HaeIII GGCC 1 cut(s) 33
HapII CCGG 2 cut(s) 30, 815
HgaI GACGC 1 cut(s) 78
HhaI GCGC 1 cut(s) 487
Hin1I GRCGYC 1 cut(s) 89
Hin1II CATG 2 cut(s) 174, 685
Hin6I GCGC 1 cut(s) 485
HinP1I GCGC 1 cut(s) 485
HincII GTYRAC 1 cut(s) 59
HindII GTYRAC 1 cut(s) 59
HindIII AAGCTT 1 cut(s) 127
HinfI GANTC 6 cut(s) 25, 201, 224, 257, 404, 776
HpaII CCGG 2 cut(s) 30, 815
Hpy166II GTNNAC 2 cut(s) 59, 635
Hpy188I TCNGA 4 cut(s) 16, 119, 745, 783
Hpy188III TCNNGA 7 cut(s) 68, 104, 416, 425, 602, 682, 831
Hpy8I GTNNAC 2 cut(s) 59, 635
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 1 cut(s) 450
HpyCH4III ACNGT 2 cut(s) 85, 701
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 3 cut(s) 527, 635, 822
HpyF10VI GCNNNNNNNGC 5 cut(s) 215, 484, 491, 553, 575
HpyF3I CTNAG 4 cut(s) 76, 210, 565, 600
HpySE526I ACGT 1 cut(s) 321
Hsp92I GRCGYC 1 cut(s) 89
Hsp92II CATG 2 cut(s) 174, 685
HspAI GCGC 1 cut(s) 485
Ksp22I TGATCA 1 cut(s) 678
Kzo9I GATC 3 cut(s) 106, 114, 678
LmnI GCTCC 2 cut(s) 17, 302
LweI GCATC 1 cut(s) 699
MaeI CTAG 4 cut(s) 68, 261, 416, 548
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 3 cut(s) 79, 149, 837
MalI GATC 3 cut(s) 108, 116, 680
MboI GATC 3 cut(s) 106, 114, 678
MboII GAAGA 3 cut(s) 260, 758, 761
MflI RGATCY 1 cut(s) 114
MhlI GDGCHC 3 cut(s) 558, 619, 637
MluCI AATT 7 cut(s) 99, 316, 473, 519, 671, 767, 823
MlyI GAGTC 1 cut(s) 785
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 7 cut(s) 56, 113, 137, 387, 609, 631, 724
MroXI GAANNNNTTC 2 cut(s) 24, 477
MseI TTAA 4 cut(s) 167, 315, 518, 522
MslI CAYNNNNRTG 1 cut(s) 702
MspI CCGG 2 cut(s) 30, 815
MspR9I CCNGG 2 cut(s) 30, 369
Mva1269I GAATGC 1 cut(s) 166
MvaI CCWGG 1 cut(s) 369
MwoI GCNNNNNNNGC 5 cut(s) 215, 484, 491, 553, 575
NciI CCSGG 1 cut(s) 30
NdeII GATC 3 cut(s) 106, 114, 678
NlaIII CATG 2 cut(s) 174, 685
NlaIV GGNNCC 2 cut(s) 461, 618
NmuCI GTSAC 1 cut(s) 79
NsbI TGCGCA 1 cut(s) 486
NspI RCATGY 1 cut(s) 174
OliI CACNNNNGTG 1 cut(s) 702
PacI TTAATTAA 1 cut(s) 522
PagI TCATGA 1 cut(s) 681
PciI ACATGT 1 cut(s) 170
PctI GAATGC 1 cut(s) 166
PdmI GAANNNNTTC 2 cut(s) 24, 477
PfeI GAWTC 5 cut(s) 25, 201, 224, 257, 404
PfoI TCCNGGA 1 cut(s) 367
PinAI ACCGGT 1 cut(s) 814
PleI GAGTC 1 cut(s) 784
PpsI GAGTC 1 cut(s) 784
PpuMI RGGWCCY 1 cut(s) 459
PscI ACATGT 1 cut(s) 170
PshBI ATTAAT 1 cut(s) 522
Psp124BI GAGCTC 1 cut(s) 558
Psp5II RGGWCCY 1 cut(s) 459
Psp6I CCWGG 1 cut(s) 367
PspGI CCWGG 1 cut(s) 367
PspN4I GGNNCC 2 cut(s) 461, 618
PspPI GGNCC 2 cut(s) 136, 459
PspPPI RGGWCCY 1 cut(s) 459
PsuI RGATCY 1 cut(s) 114
RsaI GTAC 1 cut(s) 352
RsaNI GTAC 1 cut(s) 351
RseI CAYNNNNRTG 1 cut(s) 702
SacI GAGCTC 1 cut(s) 558
SaqAI TTAA 4 cut(s) 167, 315, 518, 522
Sau3AI GATC 3 cut(s) 106, 114, 678
Sau96I GGNCC 2 cut(s) 136, 459
SchI GAGTC 1 cut(s) 785
ScrFI CCNGG 2 cut(s) 30, 369
SduI GDGCHC 3 cut(s) 558, 619, 637
SfaNI GCATC 1 cut(s) 699
SinI GGWCC 2 cut(s) 136, 459
SmiMI CAYNNNNRTG 1 cut(s) 702
SmlI CTYRAG 1 cut(s) 713
SmoI CTYRAG 1 cut(s) 713
Sse9I AATT 7 cut(s) 99, 316, 473, 519, 671, 767, 823
SsiI CCGC 1 cut(s) 264
SspMI CTAG 4 cut(s) 68, 261, 416, 548
SstI GAGCTC 1 cut(s) 558
StyD4I CCNGG 2 cut(s) 28, 367
StyI CCWWGG 1 cut(s) 139
TaaI ACNGT 2 cut(s) 85, 701
TaiI ACGT 1 cut(s) 324
TaqI TCGA 2 cut(s) 199, 255
TasI AATT 7 cut(s) 99, 316, 473, 519, 671, 767, 823
TfiI GAWTC 5 cut(s) 25, 201, 224, 257, 404
Tru1I TTAA 4 cut(s) 167, 315, 518, 522
Tru9I TTAA 4 cut(s) 167, 315, 518, 522
TscAI CASTG 4 cut(s) 90, 160, 637, 733
TseFI GTSAC 1 cut(s) 79
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 2 cut(s) 780, 816
TspRI CASTG 4 cut(s) 90, 160, 637, 733
VneI GTGCAC 1 cut(s) 633
VpaK11BI GGWCC 2 cut(s) 136, 459
VspI ATTAAT 1 cut(s) 522
XapI RAATTY 1 cut(s) 671
XbaI TCTAGA 2 cut(s) 67, 415
XceI RCATGY 1 cut(s) 174
XmnI GAANNNNTTC 2 cut(s) 24, 477
XspI CTAG 4 cut(s) 68, 261, 416, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.