Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004679

Physical Location & Seq

Genomic Coordinates Reverse (-)

31398754 .. 31402840

Gene Structure 4087 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1185300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1104 bp

ATGTCTCACGATTCTCTCCCACGTCTCTCTGAAAACCAAATCGTCCTCGGCGTCGGCGGGGTGGGCGTTGACTTTTTGGCCGCAGTGCCCTCTTATCCTAAGCCGGACGAAAAGATCAGAACCACCAGCTTAAAGGTTCAAGGAGGTGGGAATACGGGTAATGCCTTAACTTGTGCAGCTCGTTTGGGTTTGACTACGAGGGTGATTTCCAAGATTGCAAATGATACGCAAGGCAGGGCTATACTGGAGGTGCTACGGAACGATGGTGTTGATACTTCTTTTATTGTGGTTTCTGAGGAGGGTAATTCACCATTTACCTACATTATTGTAGACAACGAAACGAAAACCCGTACCTGTATTTTTACTCCAGGGTATCCTCTCATGATGCCGGATGACCTTTCCCAATCAAGTTTATCATCTGCGTTGGATGGAGCAAGAATTGTTTATTTTGATGTACGATGGCCTGAAACTGCTTTAGTTGTTGCGCAGGAGGCTGCTCGGAAAAATATACCTATATTAATTGATAATGAACGGAAAAGAGAAGGGTTGGATGATCTTATAACCTTTGCTGATTATGCTGTATGCTCAGCGAAATTTCCACAGGCATGGACAGAAGCAGCATCTGCTCCTAGTGCTCTTGTTTCGATGCTTTTGAAATTGCCAAAGCTCAAATTCGTAATTGTGACATTGGGAGAAGATGGATGCATTATGCTTGAGAGAAGTGTGGATGAGATACCAGACATAGAAGAAATGGATGTCGACAGCTTATTGGGATCGCTGAAGCAGAGAAAGAATGATAGCACAGCTATCCCAACATATGTTTCATCGCCAGTGACAAAATTGAGAGCCAACGGAATAGGGACTGTTTGTGGGAGGTTGCTGGTGGGAACAGCTGAGAAAGTACCTCCAGAAGAACTCTTAGATACAACAGGTGCTGGAGATAGTTTTGTTGGAGCAGTTCTCTATGCTATTTGCACCAATATGCCACCAGAGAAAATGCTGCCGTTTGCTGCTCAAGTGGCTGCCCTCTGCTGTAGGGCTTTAGGAGCTCGAGCTGGCCTTCCACACCGCACAGATTCACGTCTGGCATCATTTTTATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PfkB

Protein Analysis

368

Amino Acids

39.32

Weight (kDa)

5.05

Isoelectric Point (pI)

36.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PfkB	PF00294	23 - 240	6.5e-25	pfkB family carbohydrate kinase
PfkB	PF00294	299 - 356	1.5e-13	pfkB family carbohydrate kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004679)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G28706 AT4G28706 AT4G28706 AT4G28706 AT5G43910 AT5G43910 AT5G43910 AT5G43910 AT5G43910
fragaria_vesca	FvH4_2g20180 FvH4_2g20180 FvH4_2g20180 FvH4_2g20180
malus_domestica	MD05G1185300.v1.1 MD10G1173500.v1.1
prunus_persica	Prupe.8G212100_v2.0.a1 Prupe.8G212100_v2.0.a1 Prupe.8G212100_v2.0.a1
pyrus_communis	pycom05g17010 pycom10g14960
rosa_chinensis	RchiOBHm_Chr6g0285941
rosa_laevigata	RLG00000012595
rosa_multiflora	Rmu_sc0004808.1_g000008
rosa_roxburghii	Rroxscaffold_7G00182580
rosa_rugosa	Rorug06G0177600 Rorug06G0177600
rosa_samantha	Rh6AG289700 Rh6BG291600 Rh6CG292500 Rh6DG284600
rosa_wichuraiana	Rw6G024960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	560
Acc16I	TGCGCA	1 cut(s)	486
AccI	GTMKAC	2 cut(s)	330, 759
AciI	CCGC	3 cut(s)	57, 81, 1069
AclWI	GGATC	1 cut(s)	781
AcoI	YGGCCR	1 cut(s)	78
AcsI	RAATTY	2 cut(s)	593, 671
AcuI	CTGAAG	1 cut(s)	800
AcyI	GRCGYC	1 cut(s)	51
AfaI	GTAC	3 cut(s)	352, 456, 903
AgsI	TTSAA	2 cut(s)	140, 655
AjiI	CACGTC	2 cut(s)	23, 1082
AjnI	CCWGG	1 cut(s)	367
AjuI	GAANNNNNNNTTGG	2 cut(s)	805, 837
AluBI	AGCT	8 cut(s)	129, 179, 667, 765, 806, 893, 1049, 1055
AluI	AGCT	8 cut(s)	129, 179, 667, 765, 806, 893, 1049, 1055
Alw21I	GWGCWC	2 cut(s)	637, 1051
Alw26I	GTCTC	2 cut(s)	9, 29
AlwI	GGATC	1 cut(s)	781
AlwNI	CAGNNNCTG	2 cut(s)	623, 935
Ama87I	CYCGRG	1 cut(s)	1050
AoxI	GGCC	3 cut(s)	78, 461, 1057
ApeKI	GCWGC	6 cut(s)	176, 494, 617, 1000, 1010, 1022
ApoI	RAATTY	2 cut(s)	593, 671
AseI	ATTAAT	1 cut(s)	518
AspLEI	GCGC	1 cut(s)	487
AsuHPI	GGTGA	2 cut(s)	214, 300
AvaI	CYCGRG	1 cut(s)	1050
BaeGI	GKGCMC	1 cut(s)	90
BanII	GRGCYC	1 cut(s)	1051
Bbv12I	GWGCWC	2 cut(s)	637, 1051
BbvI	GCAGC	6 cut(s)	188, 481, 629, 987, 997, 1009
BccI	CCATC	4 cut(s)	257, 422, 453, 692
BceAI	ACGGC	1 cut(s)	988
BciT130I	CCWGG	1 cut(s)	369
BciVI	GTATCC	1 cut(s)	384
BcoDI	GTCTC	2 cut(s)	9, 29
BfaI	CTAG	1 cut(s)	630
BfmI	CTRYAG	1 cut(s)	1033
BfuI	GTATCC	1 cut(s)	384
BisI	GCNGC	7 cut(s)	81, 177, 495, 618, 1001, 1011, 1023
BlpI	GCTNAGC	1 cut(s)	586
BlsI	GCNGC	7 cut(s)	82, 178, 496, 619, 1002, 1012, 1024
Bme1390I	CCNGG	1 cut(s)	369
BmeT110I	CYCGRG	1 cut(s)	1050
BmgBI	CACGTC	2 cut(s)	23, 1082
BmrFI	CCNGG	1 cut(s)	369
BmsI	GCATC	5 cut(s)	375, 629, 636, 692, 1097
BplI	GAGNNNNNCTC	2 cut(s)	945, 977
BpmI	CTGGAG	4 cut(s)	266, 351, 891, 957
Bpu10I	CCTNAGC	1 cut(s)	99
Bpu1102I	GCTNAGC	1 cut(s)	586
BpuEI	CTTGAG	2 cut(s)	734, 999
BsaHI	GRCGYC	1 cut(s)	51
BsaJI	CCNNGG	2 cut(s)	46, 368
Bse1I	ACTGG	2 cut(s)	249, 830
BseBI	CCWGG	1 cut(s)	369
BseDI	CCNNGG	2 cut(s)	46, 368
BseGI	GGATG	6 cut(s)	397, 433, 556, 707, 733, 760
BseMII	CTCAG	3 cut(s)	285, 600, 885
BseNI	ACTGG	2 cut(s)	249, 830
BseRI	GAGGAG	1 cut(s)	311
BseSI	GKGCMC	1 cut(s)	90
BseXI	GCAGC	6 cut(s)	188, 481, 629, 987, 997, 1009
BsgI	GTGCAG	1 cut(s)	195
BshFI	GGCC	3 cut(s)	80, 463, 1059
BsiHKAI	GWGCWC	2 cut(s)	637, 1051
BsiHKCI	CYCGRG	1 cut(s)	1050
BsiSI	CCGG	2 cut(s)	104, 389
BslFI	GGGAC	1 cut(s)	874
BsmAI	GTCTC	2 cut(s)	9, 29
BsmBI	CGTCTC	1 cut(s)	29
BsmFI	GGGAC	1 cut(s)	874
BsnI	GGCC	3 cut(s)	80, 463, 1059
BsoBI	CYCGRG	1 cut(s)	1050
Bsp1286I	GDGCHC	3 cut(s)	90, 637, 1051
Bsp143I	GATC	3 cut(s)	114, 553, 773
Bsp1720I	GCTNAGC	1 cut(s)	586
BspACI	CCGC	3 cut(s)	57, 81, 1069
BspANI	GGCC	3 cut(s)	80, 463, 1059
BspCNI	CTCAG	3 cut(s)	286, 599, 886
BspHI	TCATGA	1 cut(s)	381
BspPI	GGATC	1 cut(s)	781
BsrI	ACTGG	2 cut(s)	249, 830
BssECI	CCNNGG	2 cut(s)	46, 368
BssMI	GATC	3 cut(s)	114, 553, 773
BssNI	GRCGYC	1 cut(s)	51
Bst2UI	CCWGG	1 cut(s)	369
Bst4CI	ACNGT	1 cut(s)	865
BstACI	GRCGYC	1 cut(s)	51
BstAPI	GCANNNNNTGC	1 cut(s)	623
BstC8I	GCNNGC	1 cut(s)	1057
BstDEI	CTNAG	5 cut(s)	99, 294, 586, 894, 919
BstF5I	GGATG	6 cut(s)	397, 433, 556, 707, 733, 760
BstHHI	GCGC	1 cut(s)	487
BstKTI	GATC	3 cut(s)	117, 556, 776
BstMAI	GTCTC	2 cut(s)	9, 29
BstMBI	GATC	3 cut(s)	114, 553, 773
BstMWI	GCNNNNNNNGC	7 cut(s)	63, 491, 575, 623, 632, 1019, 1046
BstNI	CCWGG	1 cut(s)	369
BstSCI	CCNGG	1 cut(s)	367
BstSFI	CTRYAG	1 cut(s)	1033
BstSLI	GKGCMC	1 cut(s)	90
BstV1I	GCAGC	6 cut(s)	188, 481, 629, 987, 997, 1009
BstXI	CCANNNNNNTGG	1 cut(s)	606
BsuI	GTATCC	1 cut(s)	384
BsuRI	GGCC	3 cut(s)	80, 463, 1059
BtgZI	GCGATG	1 cut(s)	810
BtrI	CACGTC	2 cut(s)	23, 1082
BtsCI	GGATG	6 cut(s)	397, 433, 556, 707, 733, 760
BtsI	GCAGTG	1 cut(s)	90
BtsIMutI	CAGTG	2 cut(s)	90, 837
Cac8I	GCNNGC	1 cut(s)	1057
CaiI	CAGNNNCTG	2 cut(s)	623, 935
CciI	TCATGA	1 cut(s)	381
CfoI	GCGC	1 cut(s)	487
CseI	GACGC	1 cut(s)	40
Csp6I	GTAC	3 cut(s)	351, 455, 902
CviAII	CATG	2 cut(s)	382, 606
CviQI	GTAC	3 cut(s)	351, 455, 902
DdeI	CTNAG	5 cut(s)	99, 294, 586, 894, 919
DpnI	GATC	3 cut(s)	116, 555, 775
DpnII	GATC	3 cut(s)	114, 553, 773
EaeI	YGGCCR	1 cut(s)	78
Ecl136II	GAGCTC	1 cut(s)	1049
Eco24I	GRGCYC	1 cut(s)	1051
Eco53kI	GAGCTC	1 cut(s)	1049
Eco57I	CTGAAG	1 cut(s)	800
Eco88I	CYCGRG	1 cut(s)	1050
EcoICRI	GAGCTC	1 cut(s)	1049
EcoRII	CCWGG	1 cut(s)	367
EcoT22I	ATGCAT	1 cut(s)	707
EcoT38I	GRGCYC	1 cut(s)	1051
Esp3I	CGTCTC	1 cut(s)	29
FaeI	CATG	2 cut(s)	385, 609
FaqI	GGGAC	1 cut(s)	874
FatI	CATG	2 cut(s)	381, 605
FauI	CCCGC	1 cut(s)	50
FauNDI	CATATG	1 cut(s)	817
FblI	GTMKAC	2 cut(s)	330, 759
Fnu4HI	GCNGC	7 cut(s)	81, 177, 495, 618, 1001, 1011, 1023
FokI	GGATG	6 cut(s)	404, 440, 563, 714, 740, 767
FriOI	GRGCYC	1 cut(s)	1051
Fsp4HI	GCNGC	7 cut(s)	81, 177, 495, 618, 1001, 1011, 1023
FspBI	CTAG	1 cut(s)	630
FspI	TGCGCA	1 cut(s)	486
GlaI	GCGC	1 cut(s)	486
GluI	GCNGC	7 cut(s)	81, 177, 495, 618, 1001, 1011, 1023
GsuI	CTGGAG	4 cut(s)	266, 351, 891, 957
HaeIII	GGCC	3 cut(s)	80, 463, 1059
HapII	CCGG	2 cut(s)	104, 389
HgaI	GACGC	1 cut(s)	40
HhaI	GCGC	1 cut(s)	487
Hin1I	GRCGYC	1 cut(s)	51
Hin1II	CATG	2 cut(s)	385, 609
Hin6I	GCGC	1 cut(s)	485
HinP1I	GCGC	1 cut(s)	485
HincII	GTYRAC	2 cut(s)	70, 760
HindII	GTYRAC	2 cut(s)	70, 760
HinfI	GANTC	2 cut(s)	11, 1076
HpaII	CCGG	2 cut(s)	104, 389
HphI	GGTGA	2 cut(s)	214, 300
Hpy166II	GTNNAC	3 cut(s)	70, 331, 760
Hpy188I	TCNGA	4 cut(s)	31, 119, 295, 501
Hpy188III	TCNNGA	3 cut(s)	8, 382, 908
Hpy8I	GTNNAC	3 cut(s)	70, 331, 760
Hpy99I	CGWCG	1 cut(s)	56
HpyAV	CCTTC	2 cut(s)	536, 1070
HpyCH4III	ACNGT	1 cut(s)	865
HpyCH4IV	ACGT	2 cut(s)	22, 1081
HpyCH4V	TGCA	4 cut(s)	176, 218, 705, 975
HpyF10VI	GCNNNNNNNGC	7 cut(s)	63, 491, 575, 623, 632, 1019, 1046
HpyF3I	CTNAG	5 cut(s)	99, 294, 586, 894, 919
HpySE526I	ACGT	2 cut(s)	22, 1081
Hsp92I	GRCGYC	1 cut(s)	51
Hsp92II	CATG	2 cut(s)	385, 609
HspAI	GCGC	1 cut(s)	485
Kzo9I	GATC	3 cut(s)	114, 553, 773
LmnI	GCTCC	4 cut(s)	431, 631, 953, 1046
Lsp1109I	GCAGC	6 cut(s)	188, 481, 629, 987, 997, 1009
LweI	GCATC	5 cut(s)	375, 629, 636, 692, 1097
MaeI	CTAG	1 cut(s)	630
MaeII	ACGT	2 cut(s)	22, 1081
MaeIII	GTNAC	2 cut(s)	682, 832
MalI	GATC	3 cut(s)	116, 555, 775
MboI	GATC	3 cut(s)	114, 553, 773
MboII	GAAGA	3 cut(s)	707, 758, 923
MhlI	GDGCHC	3 cut(s)	90, 637, 1051
MluCI	AATT	8 cut(s)	304, 438, 519, 593, 656, 671, 678, 839
MmeI	TCCRAC	3 cut(s)	405, 528, 931
Mph1103I	ATGCAT	1 cut(s)	707
MseI	TTAA	4 cut(s)	131, 167, 518, 1102
MslI	CAYNNNNRTG	2 cut(s)	604, 980
MspA1I	CMGCKG	1 cut(s)	893
MspI	CCGG	2 cut(s)	104, 389
MspR9I	CCNGG	1 cut(s)	369
MvaI	CCWGG	1 cut(s)	369
MwoI	GCNNNNNNNGC	7 cut(s)	63, 491, 575, 623, 632, 1019, 1046
NdeI	CATATG	1 cut(s)	817
NdeII	GATC	3 cut(s)	114, 553, 773
NlaIII	CATG	2 cut(s)	385, 609
NmeAIII	GCCGAG	1 cut(s)	27
NmuCI	GTSAC	2 cut(s)	682, 832
NsbI	TGCGCA	1 cut(s)	486
NsiI	ATGCAT	1 cut(s)	707
PaeR7I	CTCGAG	1 cut(s)	1050
PagI	TCATGA	1 cut(s)	381
PcsI	WCGNNNNNNNCGW	1 cut(s)	48
PfeI	GAWTC	2 cut(s)	11, 1076
PkrI	GCNGC	7 cut(s)	82, 178, 496, 619, 1002, 1012, 1024
PshBI	ATTAAT	1 cut(s)	518
PsiI	TTATAA	1 cut(s)	560
Psp124BI	GAGCTC	1 cut(s)	1051
Psp6I	CCWGG	1 cut(s)	367
PspGI	CCWGG	1 cut(s)	367
PspXI	VCTCGAGB	1 cut(s)	1050
PstNI	CAGNNNCTG	2 cut(s)	623, 935
PvuII	CAGCTG	1 cut(s)	893
RsaI	GTAC	3 cut(s)	352, 456, 903
RsaNI	GTAC	3 cut(s)	351, 455, 902
RseI	CAYNNNNRTG	2 cut(s)	604, 980
SacI	GAGCTC	1 cut(s)	1051
SalI	GTCGAC	1 cut(s)	758
SaqAI	TTAA	4 cut(s)	131, 167, 518, 1102
SatI	GCNGC	7 cut(s)	81, 177, 495, 618, 1001, 1011, 1023
Sau3AI	GATC	3 cut(s)	114, 553, 773
ScrFI	CCNGG	1 cut(s)	369
SduI	GDGCHC	3 cut(s)	90, 637, 1051
SfaNI	GCATC	5 cut(s)	375, 629, 636, 692, 1097
SfcI	CTRYAG	1 cut(s)	1033
Sfr274I	CTCGAG	1 cut(s)	1050
SlaI	CTCGAG	1 cut(s)	1050
SmiMI	CAYNNNNRTG	2 cut(s)	604, 980
SmlI	CTYRAG	3 cut(s)	713, 1014, 1050
SmoI	CTYRAG	3 cut(s)	713, 1014, 1050
Sse9I	AATT	8 cut(s)	304, 438, 519, 593, 656, 671, 678, 839
SsiI	CCGC	3 cut(s)	57, 81, 1069
SspMI	CTAG	1 cut(s)	630
SstI	GAGCTC	1 cut(s)	1051
StyD4I	CCNGG	1 cut(s)	367
TaaI	ACNGT	1 cut(s)	865
TaiI	ACGT	2 cut(s)	25, 1084
TaqI	TCGA	3 cut(s)	644, 759, 1051
TasI	AATT	8 cut(s)	304, 438, 519, 593, 656, 671, 678, 839
TauI	GCSGC	1 cut(s)	83
TfiI	GAWTC	2 cut(s)	11, 1076
Tru1I	TTAA	4 cut(s)	131, 167, 518, 1102
Tru9I	TTAA	4 cut(s)	131, 167, 518, 1102
TscAI	CASTG	2 cut(s)	90, 837
TseFI	GTSAC	2 cut(s)	682, 832
TseI	GCWGC	6 cut(s)	176, 494, 617, 1000, 1010, 1022
Tsp45I	GTSAC	2 cut(s)	682, 832
TspDTI	ATGAA	2 cut(s)	543, 813
TspGWI	ACGGA	3 cut(s)	271, 547, 867
TspRI	CASTG	2 cut(s)	90, 837
VspI	ATTAAT	1 cut(s)	518
XapI	RAATTY	2 cut(s)	593, 671
XhoI	CTCGAG	1 cut(s)	1050
XmiI	GTMKAC	2 cut(s)	330, 759
XspI	CTAG	1 cut(s)	630
Zsp2I	ATGCAT	1 cut(s)	707

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.