Rh6DG284600

Belongs to the carbohydrate kinase PfkB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0004679
Physical Location & Seq
Reverse (-)
47948577 .. 47953938
5362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG284600.1

Sequence Viewer

Length: 1044 bp
ATGCTGTCTCTCGTTTACACCACCCCAAAAGCCAATTCCCCCTCTCCTCTCGTCAGCTTACCCCACTATTCTTCTTCTCCTCCTCCTTTTCAGACTTCACCTTCAAAGACGCACCACACCCTATCTCTACCTTCTTCACCACGTCGCTCAACCAGGTGCAGAATGTCGTCCTCCGATTCGCTTCCCCCTCTCCCGGAAAACCGGACCGTGCTCGGTGTGGGCAGCATGGGGCTGGATTTCTTGGCGGTCGTGTCCTCTTATCCTAAGCCGGACGATAAGATCAGGACCACCAGCTTGAAGGTTCAAGGAGGTGGAAATGCCAGTAATGCTTTAACTTGTGTGGCTCGTTTGGGGCTAAGTCCCAGGGTCATTTCCAAGATTGCGGATGATACACAAGGCAGGGGTATAATGGAGGAGCTACAAGCCGAAGGCGTAGATACTTCGTTTGTTGTGGTTGCTGAGGAGGGTAATTCACCATTTACCTACATTATTGTTGACAATGAAACGAATACTCGTACGTGTATTCATACTGAAGGATATCCTCCTATGATACCGGATGACCTTTCACCTTCAAGTTTATCGTCTGCACTGGATGGAGCAAGACTTGCTTATTTTGATGGACGTCTACATGAAACTGCTTTACTTGTTGCACAGGAGGCTACTCGCCGGAACATACCTATATTAATTGAGGCTGAAAGGATAAGAGAAGGGCTGGATGATCTTCTAAACTTGTCTGAGTATGCCATATGCTCAGTAAAATTTCCAAAGGCATGGACAGGAGCAGAAACTTTTCCAAGTGCTCTTGTTTCAATGCTTTTGAAATTGCCAAAGCTCAAATTTGCAATTGTGACCTTGGGTGAAGATGGATGCATAATGCTTGAGAGAAATCTAGAAGAGGCTCCTCAGACGGAAGAAATTGACGTGGACAGCTTATTCGAAGCACTGAAGCAGAGAAAGGATGACAGCTTAACCAAACCAACATATGTTTCATCTGTAAGCCACTTTCTAATTACATTAAAAGTATACTCCTTTTGCTTTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

347

Amino Acids

37.94

Weight (kDa)

5.37

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 71 - 296 7.3e-22 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 625
AccI GTMKAC 2 cut(s) 625, 1023
AciI CCGC 2 cut(s) 245, 383
AcsI RAATTY 2 cut(s) 758, 836
AcuI CTGAAG 2 cut(s) 552, 965
AcyI GRCGYC 1 cut(s) 622
AfaI GTAC 1 cut(s) 517
AfiI CCNNNNNNNGG 1 cut(s) 193
AflIII ACRYGT 1 cut(s) 518
AgsI TTSAA 6 cut(s) 105, 298, 305, 573, 810, 820
AjiI CACGTC 2 cut(s) 143, 922
AjnI CCWGG 2 cut(s) 152, 362
AjuI GAANNNNNNNTTGG 4 cut(s) 19, 51, 970, 1002
AluBI AGCT 6 cut(s) 57, 294, 418, 832, 930, 966
AluI AGCT 6 cut(s) 57, 294, 418, 832, 930, 966
Alw21I GWGCWC 2 cut(s) 213, 802
Alw26I GTCTC 1 cut(s) 12
ApeKI GCWGC 1 cut(s) 222
ApoI RAATTY 2 cut(s) 758, 836
ArsI GACNNNNNNTTYG 2 cut(s) 917, 949
AseI ATTAAT 1 cut(s) 683
Asp700I GAANNNNTTC 1 cut(s) 789
AspS9I GGNCC 2 cut(s) 204, 285
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 5 cut(s) 90, 129, 465, 558, 869
AsuII TTCGAA 1 cut(s) 936
AvaII GGWCC 2 cut(s) 204, 285
Bbv12I GWGCWC 2 cut(s) 213, 802
BbvCI CCTCAGC 1 cut(s) 459
BbvI GCAGC 1 cut(s) 234
BccI CCATC 3 cut(s) 587, 611, 857
BciT130I CCWGG 2 cut(s) 154, 364
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 1 cut(s) 12
BfaI CTAG 1 cut(s) 890
BisI GCNGC 1 cut(s) 223
BlsI GCNGC 1 cut(s) 224
Bme1390I CCNGG 3 cut(s) 154, 194, 364
Bme18I GGWCC 2 cut(s) 204, 285
BmgBI CACGTC 2 cut(s) 143, 922
BmgT120I GGNCC 2 cut(s) 204, 285
BmiI GGNNCC 1 cut(s) 900
BmrFI CCNGG 3 cut(s) 154, 194, 364
BmsI GCATC 1 cut(s) 857
Bpu10I CCTNAGC 2 cut(s) 264, 459
Bpu14I TTCGAA 1 cut(s) 936
BpuEI CTTGAG 1 cut(s) 899
BpuMI CCSGG 1 cut(s) 194
BsaAI YACGTR 1 cut(s) 519
BsaHI GRCGYC 1 cut(s) 622
BsaJI CCNNGG 3 cut(s) 362, 363, 852
BsaWI WCCGGW 2 cut(s) 201, 553
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse1I ACTGG 2 cut(s) 321, 594
BseBI CCWGG 2 cut(s) 154, 364
BseDI CCNNGG 3 cut(s) 362, 363, 852
BseGI GGATG 6 cut(s) 391, 562, 598, 721, 872, 964
BseLI CCNNNNNNNGG 1 cut(s) 193
BseMII CTCAG 4 cut(s) 450, 726, 765, 917
BseNI ACTGG 2 cut(s) 321, 594
BseRI GAGGAG 6 cut(s) 36, 69, 72, 428, 476, 891
BseXI GCAGC 1 cut(s) 234
BsgI GTGCAG 2 cut(s) 178, 570
Bsh1285I CGRYCG 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 249
BsiHKAI GWGCWC 2 cut(s) 213, 802
BsiSI CCGG 5 cut(s) 194, 202, 269, 554, 667
BsiWI CGTACG 1 cut(s) 515
BslFI GGGAC 1 cut(s) 345
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 1 cut(s) 12
BsmFI GGGAC 1 cut(s) 345
Bsp119I TTCGAA 1 cut(s) 936
Bsp1286I GDGCHC 2 cut(s) 213, 802
Bsp143I GATC 2 cut(s) 279, 718
BspACI CCGC 2 cut(s) 245, 383
BspCNI CTCAG 4 cut(s) 451, 727, 764, 916
BspLI GGNNCC 1 cut(s) 900
BspT104I TTCGAA 1 cut(s) 936
BsrI ACTGG 2 cut(s) 321, 594
BssECI CCNNGG 3 cut(s) 362, 363, 852
BssMI GATC 2 cut(s) 279, 718
BssNAI GTATAC 1 cut(s) 1024
BssNI GRCGYC 1 cut(s) 622
BssT1I CCWWGG 1 cut(s) 852
Bst1107I GTATAC 1 cut(s) 1024
Bst2UI CCWGG 2 cut(s) 154, 364
Bst4CI ACNGT 1 cut(s) 208
Bst6I CTCTTC 1 cut(s) 888
BstACI GRCGYC 1 cut(s) 622
BstAPI GCANNNNNTGC 1 cut(s) 605
BstBAI YACGTR 1 cut(s) 519
BstBI TTCGAA 1 cut(s) 936
BstDEI CTNAG 6 cut(s) 264, 356, 459, 735, 751, 903
BstF5I GGATG 6 cut(s) 391, 562, 598, 721, 872, 964
BstKTI GATC 2 cut(s) 282, 721
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 2 cut(s) 279, 718
BstMCI CGRYCG 1 cut(s) 249
BstMWI GCNNNNNNNGC 3 cut(s) 326, 605, 656
BstNI CCWGG 2 cut(s) 154, 364
BstSCI CCNGG 3 cut(s) 152, 192, 362
BstV1I GCAGC 1 cut(s) 234
BstXI CCANNNNNNTGG 1 cut(s) 771
BstZ17I GTATAC 1 cut(s) 1024
BtrI CACGTC 2 cut(s) 143, 922
BtsCI GGATG 6 cut(s) 391, 562, 598, 721, 872, 964
BtsIMutI CAGTG 2 cut(s) 587, 941
Cfr13I GGNCC 2 cut(s) 204, 285
CpoI CGGWCCG 1 cut(s) 204
CseI GACGC 1 cut(s) 118
CsiI ACCWGGT 1 cut(s) 152
Csp6I GTAC 1 cut(s) 516
CspI CGGWCCG 1 cut(s) 204
CviAII CATG 3 cut(s) 226, 629, 771
CviQI GTAC 1 cut(s) 516
DdeI CTNAG 6 cut(s) 264, 356, 459, 735, 751, 903
DpnI GATC 2 cut(s) 281, 720
DpnII GATC 2 cut(s) 279, 718
Eam1104I CTCTTC 1 cut(s) 888
EarI CTCTTC 1 cut(s) 888
Eco130I CCWWGG 1 cut(s) 852
Eco32I GATATC 1 cut(s) 539
Eco47I GGWCC 2 cut(s) 204, 285
Eco57I CTGAAG 2 cut(s) 552, 965
EcoRII CCWGG 2 cut(s) 152, 362
EcoRV GATATC 1 cut(s) 539
EcoT14I CCWWGG 1 cut(s) 852
EcoT22I ATGCAT 1 cut(s) 872
ErhI CCWWGG 1 cut(s) 852
FaeI CATG 3 cut(s) 229, 632, 774
FalI AAGNNNNNCTT 2 cut(s) 592, 624
FaqI GGGAC 1 cut(s) 345
FatI CATG 3 cut(s) 225, 628, 770
FauNDI CATATG 2 cut(s) 746, 982
FblI GTMKAC 2 cut(s) 625, 1023
Fnu4HI GCNGC 1 cut(s) 223
FokI GGATG 6 cut(s) 398, 569, 605, 728, 879, 971
Fsp4HI GCNGC 1 cut(s) 223
FspBI CTAG 1 cut(s) 890
GluI GCNGC 1 cut(s) 223
HapII CCGG 5 cut(s) 194, 202, 269, 554, 667
HgaI GACGC 1 cut(s) 118
Hin1I GRCGYC 1 cut(s) 622
Hin1II CATG 3 cut(s) 229, 632, 774
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HinfI GANTC 1 cut(s) 176
HpaII CCGG 5 cut(s) 194, 202, 269, 554, 667
HphI GGTGA 5 cut(s) 90, 129, 465, 558, 869
Hpy166II GTNNAC 5 cut(s) 16, 496, 626, 925, 1024
Hpy188I TCNGA 4 cut(s) 93, 175, 736, 906
Hpy188III TCNNGA 2 cut(s) 283, 890
Hpy8I GTNNAC 5 cut(s) 16, 496, 626, 925, 1024
Hpy99I CGWCG 1 cut(s) 147
HpyAV CCTTC 7 cut(s) 111, 141, 292, 422, 527, 579, 701
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 4 cut(s) 142, 518, 622, 921
HpyCH4V TGCA 5 cut(s) 159, 587, 650, 842, 870
HpyF10VI GCNNNNNNNGC 3 cut(s) 326, 605, 656
HpyF3I CTNAG 6 cut(s) 264, 356, 459, 735, 751, 903
HpySE526I ACGT 4 cut(s) 142, 518, 622, 921
Hsp92I GRCGYC 1 cut(s) 622
Hsp92II CATG 3 cut(s) 229, 632, 774
Kzo9I GATC 2 cut(s) 279, 718
LmnI GCTCC 4 cut(s) 415, 596, 779, 904
Lsp1109I GCAGC 1 cut(s) 234
LweI GCATC 1 cut(s) 857
MabI ACCWGGT 1 cut(s) 152
MaeI CTAG 1 cut(s) 890
MaeII ACGT 4 cut(s) 142, 518, 622, 921
MaeIII GTNAC 1 cut(s) 847
MalI GATC 2 cut(s) 281, 720
MboI GATC 2 cut(s) 279, 718
MboII GAAGA 7 cut(s) 63, 66, 126, 713, 872, 905, 923
MfeI CAATTG 1 cut(s) 843
MhlI GDGCHC 2 cut(s) 213, 802
MluCI AATT 9 cut(s) 34, 469, 684, 758, 821, 836, 843, 915, 1008
Mph1103I ATGCAT 1 cut(s) 872
MroXI GAANNNNTTC 1 cut(s) 789
MseI TTAA 4 cut(s) 332, 683, 968, 1016
MspI CCGG 5 cut(s) 194, 202, 269, 554, 667
MspR9I CCNGG 3 cut(s) 154, 194, 364
MunI CAATTG 1 cut(s) 843
MvaI CCWGG 2 cut(s) 154, 364
MwoI GCNNNNNNNGC 3 cut(s) 326, 605, 656
NciI CCSGG 1 cut(s) 194
NdeI CATATG 2 cut(s) 746, 982
NdeII GATC 2 cut(s) 279, 718
NlaIII CATG 3 cut(s) 229, 632, 774
NlaIV GGNNCC 1 cut(s) 900
NmuCI GTSAC 1 cut(s) 847
NsiI ATGCAT 1 cut(s) 872
NspV TTCGAA 1 cut(s) 936
PasI CCCWGGG 1 cut(s) 363
PdmI GAANNNNTTC 1 cut(s) 789
PfeI GAWTC 1 cut(s) 176
Pfl23II CGTACG 1 cut(s) 515
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 1 cut(s) 224
Ppu21I YACGTR 1 cut(s) 519
PshBI ATTAAT 1 cut(s) 683
Psp6I CCWGG 2 cut(s) 152, 362
PspGI CCWGG 2 cut(s) 152, 362
PspLI CGTACG 1 cut(s) 515
PspN4I GGNNCC 1 cut(s) 900
PspPI GGNCC 2 cut(s) 204, 285
RsaI GTAC 1 cut(s) 517
RsaNI GTAC 1 cut(s) 516
Rsr2I CGGWCCG 1 cut(s) 204
RsrII CGGWCCG 1 cut(s) 204
SaqAI TTAA 4 cut(s) 332, 683, 968, 1016
SatI GCNGC 1 cut(s) 223
Sau3AI GATC 2 cut(s) 279, 718
Sau96I GGNCC 2 cut(s) 204, 285
ScrFI CCNGG 3 cut(s) 154, 194, 364
SduI GDGCHC 2 cut(s) 213, 802
SexAI ACCWGGT 1 cut(s) 152
SfaNI GCATC 1 cut(s) 857
SfuI TTCGAA 1 cut(s) 936
SinI GGWCC 2 cut(s) 204, 285
SmlI CTYRAG 1 cut(s) 878
SmoI CTYRAG 1 cut(s) 878
Sse9I AATT 9 cut(s) 34, 469, 684, 758, 821, 836, 843, 915, 1008
SsiI CCGC 2 cut(s) 245, 383
SspMI CTAG 1 cut(s) 890
StyD4I CCNGG 3 cut(s) 152, 192, 362
StyI CCWWGG 1 cut(s) 852
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 4 cut(s) 145, 521, 625, 924
TaqI TCGA 1 cut(s) 936
TasI AATT 9 cut(s) 34, 469, 684, 758, 821, 836, 843, 915, 1008
TfiI GAWTC 1 cut(s) 176
Tru1I TTAA 4 cut(s) 332, 683, 968, 1016
Tru9I TTAA 4 cut(s) 332, 683, 968, 1016
TscAI CASTG 2 cut(s) 594, 948
TseFI GTSAC 1 cut(s) 847
TseI GCWGC 1 cut(s) 222
Tsp45I GTSAC 1 cut(s) 847
TspDTI ATGAA 4 cut(s) 515, 516, 645, 978
TspGWI ACGGA 1 cut(s) 923
TspRI CASTG 2 cut(s) 594, 948
VpaK11BI GGWCC 2 cut(s) 204, 285
VspI ATTAAT 1 cut(s) 683
XapI RAATTY 2 cut(s) 758, 836
XbaI TCTAGA 1 cut(s) 889
XmiI GTMKAC 2 cut(s) 625, 1023
XmnI GAANNNNTTC 1 cut(s) 789
XspI CTAG 1 cut(s) 890
ZraI GACGTC 1 cut(s) 623
Zsp2I ATGCAT 1 cut(s) 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.