Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004679

Physical Location & Seq

Genomic Coordinates Reverse (-)

26626751 .. 26631077

Gene Structure 4327 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1173500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1200 bp

ATGCTTTCTTTCTCCCCAACTTCTAATGCCACTCTTCTCAACTCACCAACATCCTTCTCCCTCCCTCCCTCCCCACCTCGCTCCGCCAGGTTCAAAATGTCAATCGATTCTCTCCCTCCTCTCCCCGAAAACCGAACCGTCGTCGGCGTCGGCGCGGTGGTCGTTGATTTTTTGGCCGCAGTGGCCTCTTATCCTAAGCCGGACGATAAAATCAGATCCACCAGCTTAAAGGTTCAAGGAGGTGGGAATGCAGGTAATGCCTTTACCTGCGCAGCTCGTTTGGGTTTGAATGCCAGGCTGATTTCCAAGGTTGCAAATGATACACAAGGCAGGGGTATACTGGAGGGGCTGCAGGATGACGGCATTGATACTTCTTTTGTTGTGGTTTCCGAGGAGGGTAATTCACCATTTACCTACATTATTGTTGACAACGAAACGAAAACTCGCACCTGTATTCATACTCCAGGATATCCTCCCATGATACCAGATGACCTTTCCCAATCAAGTTTATCATCTGCGTTGGATGGAGCAAGAATTCTTTATTTAGATTGCCGAATACATGAAACTGCATTAGTTGTTGCGCAGGAGGCTGCTCGGAAAAATATACCTATATTAGTTGATGGTGAACGGCTAAGAGAAGGGTTGGATGGTCTTCTAAACTTGGCTGATTATGCTGTATGCTCAGCGAAATTTCCGCAGGCATGGACAGAAGCAGCATCTGTTTCTAGTGCTCTTGTTTCAATGCTTTTGAAATTGCCAAAGATCAAATTTGCAATTGTGACATTGGGAGAAGATGGATGCATTATGCTTGAGAGAAGTGTGGACGAAACTCCTCAGATAGAAGAAATGGATGTCGACAGCTTGTTGGAATCACTGAAGCAGAGAAAGGATGATAGCACAGCTATCCCAACATGTGTTTCATCGCCGGTGACAAAATTGAGAGCCAACGGAATAGGGACAGTTTGTGGGAGGTTGTTGGTTGGAACAGCTGAGAAAGTACCGCCGGAAGAACTCGTAGACACAACAGGTGCTGGAGATAGTTTTATTGGAGCAGTTCTTTATGCTATTTGCACCAACATGCCACCAGAGAAAATGCTGCCGTTTGCTGCACAAGTGGCAGGTGCCTGCTGTAGGGCTTTAGGAGCTCGAACTGGCCTTCCACATCGCACAGATGCACGCCTGGCATCATTTCTATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PfkB

Protein Analysis

400

Amino Acids

42.28

Weight (kDa)

5.13

Isoelectric Point (pI)

39.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PfkB	PF00294	46 - 273	2.3e-24	pfkB family carbohydrate kinase
PfkB	PF00294	331 - 388	1.9e-13	pfkB family carbohydrate kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004679)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G28706 AT4G28706 AT4G28706 AT4G28706 AT5G43910 AT5G43910 AT5G43910 AT5G43910 AT5G43910
fragaria_vesca	FvH4_2g20180 FvH4_2g20180 FvH4_2g20180 FvH4_2g20180
malus_domestica	MD05G1185300.v1.1 MD10G1173500.v1.1
prunus_persica	Prupe.8G212100_v2.0.a1 Prupe.8G212100_v2.0.a1 Prupe.8G212100_v2.0.a1
pyrus_communis	pycom05g17010 pycom10g14960
rosa_chinensis	RchiOBHm_Chr6g0285941
rosa_laevigata	RLG00000012595
rosa_multiflora	Rmu_sc0004808.1_g000008
rosa_roxburghii	Rroxscaffold_7G00182580
rosa_rugosa	Rorug06G0177600 Rorug06G0177600
rosa_samantha	Rh6AG289700 Rh6BG291600 Rh6CG292500 Rh6DG284600
rosa_wichuraiana	Rw6G024960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1109
Acc16I	TGCGCA	2 cut(s)	271, 582
Acc36I	ACCTGC	3 cut(s)	242, 275, 1109
AccB1I	GGYRCC	1 cut(s)	1121
AccI	GTMKAC	3 cut(s)	337, 855, 1017
AccII	CGCG	1 cut(s)	155
AciI	CCGC	5 cut(s)	84, 155, 177, 695, 1001
AclWI	GGATC	1 cut(s)	210
AcoI	YGGCCR	1 cut(s)	174
AcsI	RAATTY	3 cut(s)	534, 689, 767
AcuI	CTGAAG	1 cut(s)	896
AcyI	GRCGYC	1 cut(s)	147
AfaI	GTAC	1 cut(s)	999
AfiI	CCNNNNNNNGG	1 cut(s)	1131
AflIII	ACRYGT	1 cut(s)	911
AgsI	TTSAA	5 cut(s)	94, 236, 289, 741, 751
AjnI	CCWGG	4 cut(s)	86, 293, 463, 1179
AjuI	GAANNNNNNNTTGG	2 cut(s)	901, 933
AloI	GAACNNNNNNTCC	2 cut(s)	1141, 1173
AluBI	AGCT	6 cut(s)	225, 275, 861, 902, 989, 1145
AluI	AGCT	6 cut(s)	225, 275, 861, 902, 989, 1145
Alw21I	GWGCWC	2 cut(s)	733, 1147
AlwI	GGATC	1 cut(s)	210
AlwNI	CAGNNNCTG	2 cut(s)	719, 1031
AoxI	GGCC	3 cut(s)	174, 183, 1153
ApeKI	GCWGC	6 cut(s)	272, 349, 590, 713, 1096, 1106
ApoI	RAATTY	3 cut(s)	534, 689, 767
AspLEI	GCGC	3 cut(s)	155, 272, 583
AsuHPI	GGTGA	4 cut(s)	36, 396, 635, 940
BanI	GGYRCC	1 cut(s)	1121
BanII	GRGCYC	1 cut(s)	1147
BbsI	GAAGAC	1 cut(s)	644
Bbv12I	GWGCWC	2 cut(s)	733, 1147
BbvI	GCAGC	6 cut(s)	284, 336, 577, 725, 1083, 1093
BccI	CCATC	4 cut(s)	518, 614, 641, 788
BceAI	ACGGC	3 cut(s)	376, 644, 1084
BciT130I	CCWGG	4 cut(s)	88, 295, 465, 1181
BfaI	CTAG	1 cut(s)	726
BfmI	CTRYAG	2 cut(s)	350, 1129
BfuAI	ACCTGC	3 cut(s)	242, 275, 1109
BglI	GCCNNNNNGGC	1 cut(s)	182
BisI	GCNGC	7 cut(s)	177, 273, 350, 591, 714, 1097, 1107
BlpI	GCTNAGC	1 cut(s)	682
BlsI	GCNGC	7 cut(s)	178, 274, 351, 592, 715, 1098, 1108
Bme1390I	CCNGG	4 cut(s)	88, 295, 465, 1181
BmiI	GGNNCC	1 cut(s)	1123
BmrFI	CCNGG	4 cut(s)	88, 295, 465, 1181
BmsI	GCATC	4 cut(s)	725, 788, 1162, 1193
BpiI	GAAGAC	1 cut(s)	644
BpmI	CTGGAG	3 cut(s)	362, 447, 1053
Bpu10I	CCTNAGC	1 cut(s)	195
Bpu1102I	GCTNAGC	1 cut(s)	682
BpuEI	CTTGAG	1 cut(s)	830
Bsa29I	ATCGAT	1 cut(s)	105
BsaHI	GRCGYC	1 cut(s)	147
BsaJI	CCNNGG	2 cut(s)	306, 390
Bsc4I	CCNNNNNNNGG	1 cut(s)	1131
Bse118I	RCCGGY	1 cut(s)	925
Bse1I	ACTGG	2 cut(s)	345, 1156
BseBI	CCWGG	4 cut(s)	88, 295, 465, 1181
BseCI	ATCGAT	1 cut(s)	105
BseDI	CCNNGG	2 cut(s)	306, 390
BseGI	GGATG	7 cut(s)	50, 361, 529, 652, 803, 856, 895
BseLI	CCNNNNNNNGG	1 cut(s)	1131
BseMII	CTCAG	3 cut(s)	696, 848, 981
BseNI	ACTGG	2 cut(s)	345, 1156
BseRI	GAGGAG	3 cut(s)	108, 407, 822
BseXI	GCAGC	6 cut(s)	284, 336, 577, 725, 1083, 1093
BsgI	GTGCAG	1 cut(s)	1092
Bsh1236I	CGCG	1 cut(s)	155
BshFI	GGCC	3 cut(s)	176, 185, 1155
BshNI	GGYRCC	1 cut(s)	1121
BshVI	ATCGAT	1 cut(s)	105
BsiHKAI	GWGCWC	2 cut(s)	733, 1147
BsiSI	CCGG	3 cut(s)	200, 926, 1004
BslFI	GGGAC	1 cut(s)	970
BslI	CCNNNNNNNGG	1 cut(s)	1131
BsmFI	GGGAC	1 cut(s)	970
BsmI	GAATGC	2 cut(s)	253, 295
BsnI	GGCC	3 cut(s)	176, 185, 1155
Bsp1286I	GDGCHC	2 cut(s)	733, 1147
Bsp143I	GATC	2 cut(s)	215, 762
Bsp1720I	GCTNAGC	1 cut(s)	682
BspACI	CCGC	5 cut(s)	84, 155, 177, 695, 1001
BspANI	GGCC	3 cut(s)	176, 185, 1155
BspCNI	CTCAG	3 cut(s)	695, 847, 982
BspDI	ATCGAT	1 cut(s)	105
BspFNI	CGCG	1 cut(s)	155
BspLI	GGNNCC	1 cut(s)	1123
BspMAI	CTGCAG	1 cut(s)	354
BspMI	ACCTGC	3 cut(s)	242, 275, 1109
BspPI	GGATC	1 cut(s)	210
BspT107I	GGYRCC	1 cut(s)	1121
BsrFI	RCCGGY	1 cut(s)	925
BsrI	ACTGG	2 cut(s)	345, 1156
BssAI	RCCGGY	1 cut(s)	925
BssECI	CCNNGG	2 cut(s)	306, 390
BssMI	GATC	2 cut(s)	215, 762
BssNAI	GTATAC	1 cut(s)	338
BssNI	GRCGYC	1 cut(s)	147
BssT1I	CCWWGG	1 cut(s)	306
Bst1107I	GTATAC	1 cut(s)	338
Bst2UI	CCWGG	4 cut(s)	88, 295, 465, 1181
Bst4CI	ACNGT	2 cut(s)	139, 961
Bst6I	CTCTTC	1 cut(s)	39
BstACI	GRCGYC	1 cut(s)	147
BstAPI	GCANNNNNTGC	1 cut(s)	257
BstC8I	GCNNGC	3 cut(s)	699, 1126, 1177
BstDEI	CTNAG	5 cut(s)	195, 632, 682, 834, 990
BstENI	CCTNNNNNAGG	1 cut(s)	1129
BstF5I	GGATG	7 cut(s)	50, 361, 529, 652, 803, 856, 895
BstFNI	CGCG	1 cut(s)	155
BstHHI	GCGC	3 cut(s)	155, 272, 583
BstKTI	GATC	2 cut(s)	218, 765
BstMBI	GATC	2 cut(s)	215, 762
BstMWI	GCNNNNNNNGC	7 cut(s)	182, 257, 587, 671, 1115, 1142, 1181
BstNI	CCWGG	4 cut(s)	88, 295, 465, 1181
BstNSI	RCATGY	2 cut(s)	915, 1081
BstSCI	CCNGG	4 cut(s)	86, 293, 463, 1179
BstSFI	CTRYAG	2 cut(s)	350, 1129
BstUI	CGCG	1 cut(s)	155
BstV1I	GCAGC	6 cut(s)	284, 336, 577, 725, 1083, 1093
BstV2I	GAAGAC	1 cut(s)	644
BstX2I	RGATCY	1 cut(s)	215
BstYI	RGATCY	1 cut(s)	215
BstZ17I	GTATAC	1 cut(s)	338
Bsu15I	ATCGAT	1 cut(s)	105
BsuRI	GGCC	3 cut(s)	176, 185, 1155
BsuTUI	ATCGAT	1 cut(s)	105
BtgZI	GCGATG	2 cut(s)	906, 1148
BtsCI	GGATG	7 cut(s)	50, 361, 529, 652, 803, 856, 895
BtsI	GCAGTG	1 cut(s)	186
BtsIMutI	CAGTG	2 cut(s)	186, 872
BveI	ACCTGC	3 cut(s)	242, 275, 1109
Cac8I	GCNNGC	3 cut(s)	699, 1126, 1177
CaiI	CAGNNNCTG	2 cut(s)	719, 1031
CfoI	GCGC	3 cut(s)	155, 272, 583
Cfr10I	RCCGGY	1 cut(s)	925
ClaI	ATCGAT	1 cut(s)	105
CseI	GACGC	1 cut(s)	136
Csp6I	GTAC	1 cut(s)	998
CviAII	CATG	5 cut(s)	478, 560, 702, 912, 1078
CviQI	GTAC	1 cut(s)	998
DdeI	CTNAG	5 cut(s)	195, 632, 682, 834, 990
DpnI	GATC	2 cut(s)	217, 764
DpnII	GATC	2 cut(s)	215, 762
EaeI	YGGCCR	1 cut(s)	174
Eam1104I	CTCTTC	1 cut(s)	39
EarI	CTCTTC	1 cut(s)	39
EciI	GGCGGA	1 cut(s)	73
Ecl136II	GAGCTC	1 cut(s)	1145
Eco130I	CCWWGG	1 cut(s)	306
Eco24I	GRGCYC	1 cut(s)	1147
Eco32I	GATATC	1 cut(s)	470
Eco53kI	GAGCTC	1 cut(s)	1145
Eco57I	CTGAAG	1 cut(s)	896
EcoICRI	GAGCTC	1 cut(s)	1145
EcoNI	CCTNNNNNAGG	1 cut(s)	1129
EcoRI	GAATTC	1 cut(s)	534
EcoRII	CCWGG	4 cut(s)	86, 293, 463, 1179
EcoRV	GATATC	1 cut(s)	470
EcoT14I	CCWWGG	1 cut(s)	306
EcoT22I	ATGCAT	1 cut(s)	803
EcoT38I	GRGCYC	1 cut(s)	1147
ErhI	CCWWGG	1 cut(s)	306
FaeI	CATG	5 cut(s)	481, 563, 705, 915, 1081
FaqI	GGGAC	1 cut(s)	970
FatI	CATG	5 cut(s)	477, 559, 701, 911, 1077
FblI	GTMKAC	3 cut(s)	337, 855, 1017
Fnu4HI	GCNGC	7 cut(s)	177, 273, 350, 591, 714, 1097, 1107
FokI	GGATG	7 cut(s)	37, 368, 536, 659, 810, 863, 902
FriOI	GRGCYC	1 cut(s)	1147
Fsp4HI	GCNGC	7 cut(s)	177, 273, 350, 591, 714, 1097, 1107
FspBI	CTAG	1 cut(s)	726
FspI	TGCGCA	2 cut(s)	271, 582
GlaI	GCGC	3 cut(s)	154, 271, 582
GluI	GCNGC	7 cut(s)	177, 273, 350, 591, 714, 1097, 1107
GsuI	CTGGAG	3 cut(s)	362, 447, 1053
HaeIII	GGCC	3 cut(s)	176, 185, 1155
HapII	CCGG	3 cut(s)	200, 926, 1004
HgaI	GACGC	1 cut(s)	136
HhaI	GCGC	3 cut(s)	155, 272, 583
Hin1I	GRCGYC	1 cut(s)	147
Hin1II	CATG	5 cut(s)	481, 563, 705, 915, 1081
Hin6I	GCGC	3 cut(s)	153, 270, 581
HinP1I	GCGC	3 cut(s)	153, 270, 581
HincII	GTYRAC	2 cut(s)	427, 856
HindII	GTYRAC	2 cut(s)	427, 856
HinfI	GANTC	2 cut(s)	107, 869
HpaII	CCGG	3 cut(s)	200, 926, 1004
HphI	GGTGA	4 cut(s)	36, 396, 635, 940
Hpy166II	GTNNAC	6 cut(s)	338, 427, 626, 823, 856, 1018
Hpy188I	TCNGA	4 cut(s)	215, 391, 597, 837
Hpy8I	GTNNAC	6 cut(s)	338, 427, 626, 823, 856, 1018
Hpy99I	CGWCG	3 cut(s)	143, 146, 152
HpyAV	CCTTC	3 cut(s)	64, 632, 1166
HpyCH4III	ACNGT	2 cut(s)	139, 961
HpyCH4V	TGCA	9 cut(s)	251, 314, 352, 569, 773, 801, 1071, 1109, 1175
HpyF10VI	GCNNNNNNNGC	7 cut(s)	182, 257, 587, 671, 1115, 1142, 1181
HpyF3I	CTNAG	5 cut(s)	195, 632, 682, 834, 990
Hsp92I	GRCGYC	1 cut(s)	147
Hsp92II	CATG	5 cut(s)	481, 563, 705, 915, 1081
HspAI	GCGC	3 cut(s)	153, 270, 581
Kzo9I	GATC	2 cut(s)	215, 762
LmnI	GCTCC	4 cut(s)	86, 527, 1049, 1142
Lsp1109I	GCAGC	6 cut(s)	284, 336, 577, 725, 1083, 1093
LweI	GCATC	4 cut(s)	725, 788, 1162, 1193
MaeI	CTAG	1 cut(s)	726
MaeIII	GTNAC	2 cut(s)	778, 928
MalI	GATC	2 cut(s)	217, 764
MboI	GATC	2 cut(s)	215, 762
MboII	GAAGA	5 cut(s)	26, 644, 803, 854, 1019
MfeI	CAATTG	1 cut(s)	774
MflI	RGATCY	1 cut(s)	215
MhlI	GDGCHC	2 cut(s)	733, 1147
MluCI	AATT	7 cut(s)	400, 534, 689, 752, 767, 774, 935
MmeI	TCCRAC	4 cut(s)	501, 624, 846, 961
Mph1103I	ATGCAT	1 cut(s)	803
MseI	TTAA	2 cut(s)	227, 1198
MslI	CAYNNNNRTG	1 cut(s)	1076
MspA1I	CMGCKG	1 cut(s)	989
MspI	CCGG	3 cut(s)	200, 926, 1004
MspR9I	CCNGG	4 cut(s)	88, 295, 465, 1181
MunI	CAATTG	1 cut(s)	774
Mva1269I	GAATGC	2 cut(s)	253, 295
MvaI	CCWGG	4 cut(s)	88, 295, 465, 1181
MvnI	CGCG	1 cut(s)	155
MwoI	GCNNNNNNNGC	7 cut(s)	182, 257, 587, 671, 1115, 1142, 1181
NdeII	GATC	2 cut(s)	215, 762
NlaIII	CATG	5 cut(s)	481, 563, 705, 915, 1081
NlaIV	GGNNCC	1 cut(s)	1123
NmuCI	GTSAC	2 cut(s)	778, 928
NsbI	TGCGCA	2 cut(s)	271, 582
NsiI	ATGCAT	1 cut(s)	803
NspI	RCATGY	2 cut(s)	915, 1081
PaqCI	CACCTGC	1 cut(s)	1109
PciI	ACATGT	1 cut(s)	911
PctI	GAATGC	2 cut(s)	253, 295
PfeI	GAWTC	2 cut(s)	107, 869
PfoI	TCCNGGA	1 cut(s)	463
PkrI	GCNGC	7 cut(s)	178, 274, 351, 592, 715, 1098, 1108
PscI	ACATGT	1 cut(s)	911
Psp124BI	GAGCTC	1 cut(s)	1147
Psp6I	CCWGG	4 cut(s)	86, 293, 463, 1179
PspGI	CCWGG	4 cut(s)	86, 293, 463, 1179
PspN4I	GGNNCC	1 cut(s)	1123
PstI	CTGCAG	1 cut(s)	354
PstNI	CAGNNNCTG	2 cut(s)	719, 1031
PsuI	RGATCY	1 cut(s)	215
PvuII	CAGCTG	1 cut(s)	989
RsaI	GTAC	1 cut(s)	999
RsaNI	GTAC	1 cut(s)	998
RseI	CAYNNNNRTG	1 cut(s)	1076
SacI	GAGCTC	1 cut(s)	1147
SalI	GTCGAC	1 cut(s)	854
SaqAI	TTAA	2 cut(s)	227, 1198
SatI	GCNGC	7 cut(s)	177, 273, 350, 591, 714, 1097, 1107
Sau3AI	GATC	2 cut(s)	215, 762
ScrFI	CCNGG	4 cut(s)	88, 295, 465, 1181
SduI	GDGCHC	2 cut(s)	733, 1147
SfaNI	GCATC	4 cut(s)	725, 788, 1162, 1193
SfcI	CTRYAG	2 cut(s)	350, 1129
SfiI	GGCCNNNNNGGCC	1 cut(s)	182
SgrAI	CRCCGGYG	1 cut(s)	925
SmiMI	CAYNNNNRTG	1 cut(s)	1076
SmlI	CTYRAG	1 cut(s)	809
SmoI	CTYRAG	1 cut(s)	809
Sse9I	AATT	7 cut(s)	400, 534, 689, 752, 767, 774, 935
SsiI	CCGC	5 cut(s)	84, 155, 177, 695, 1001
SspMI	CTAG	1 cut(s)	726
SstI	GAGCTC	1 cut(s)	1147
StyD4I	CCNGG	4 cut(s)	86, 293, 463, 1179
StyI	CCWWGG	1 cut(s)	306
TaaI	ACNGT	2 cut(s)	139, 961
TaqI	TCGA	3 cut(s)	105, 855, 1147
TasI	AATT	7 cut(s)	400, 534, 689, 752, 767, 774, 935
TauI	GCSGC	1 cut(s)	179
TfiI	GAWTC	2 cut(s)	107, 869
Tru1I	TTAA	2 cut(s)	227, 1198
Tru9I	TTAA	2 cut(s)	227, 1198
TscAI	CASTG	2 cut(s)	186, 879
TseFI	GTSAC	2 cut(s)	778, 928
TseI	GCWGC	6 cut(s)	272, 349, 590, 713, 1096, 1106
Tsp45I	GTSAC	2 cut(s)	778, 928
TspDTI	ATGAA	3 cut(s)	446, 576, 909
TspGWI	ACGGA	1 cut(s)	963
TspRI	CASTG	2 cut(s)	186, 879
XagI	CCTNNNNNAGG	1 cut(s)	1129
XapI	RAATTY	3 cut(s)	534, 689, 767
XceI	RCATGY	2 cut(s)	915, 1081
XmiI	GTMKAC	3 cut(s)	337, 855, 1017
XspI	CTAG	1 cut(s)	726
Zsp2I	ATGCAT	1 cut(s)	803

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.