FvH4_2g20180

Belongs to the carbohydrate kinase PfkB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004679
Physical Location & Seq
Reverse (-)
16974789 .. 16979988
5200 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20180.t2

Sequence Viewer

Length: 1203 bp
ATGCTTTCACTTGTCTACACCACCCCTCTCCTCTCTTCTTCTTCAACAACTCGCCTCACCTCGTCACCACCTCGCTCTACCAGGCTCAAAATGTCGTCTTCATCGCCGTCCGATTCGCTTCCGCCTCTCCCGGAAAACCGCACCGTGCTTGGCGTCGGCAGCATGGGCCTCGATTTCTTGGCCGCCGTCTCCTCTTATCCCAAGCCCGACGATAAGATCAGGACCACCAGCTTAAAGGTTCAAGGAGGTGGAAATGCGAGTAATGCTTTGACTTGTGTGGCTCGTTTGGGGTTGAGTCCCAGGGCGATTTCAAAGGTTTCAGACGATACACAAGGCAGGGGTATATTGGAGGAGCTGCAAGCCGACGGGGTGGATACTTCTTTTGTTGTGGTTGCCGAGGAGGGTAATTCACCATTTTCCTACATTATTGTTGACAAGGAAACGAATACTCGTACTTGTATTCATACTCCAGGATATCCTCCTATGATACCGGATGACCTTTCGCCTTCAAGTTTATCGTCTGCATTGGATGGAGCAAGACTTGCTTATTTTGATGGACGTCTGCACGAAACTGCTTTACTTGTTGCTCAGGAGGCTACTCGCCGGAGCATACCTATATTAATTGAGGCTGAAAGGATAAGAGAAGGGTTGGATGATCTTCTAAACTTGGCTGAGTATGCCATATGCTCAGTGAAATTTCCGCAGGCATGGACAGAAGCGAAATCGTTTCCGAGTGCTCTTGTTTCAATGCTTTTGAAATTGCCGAAGCTCAAGTTTGCAATTGTGACCTTGGGTGAAGATGGTTGCTTAATGCTCGAGAGAAGTGTAGAAGAGCCTCCTCAGACGGAAGAAATAGACGTGGAAAGCTTATTCGAAGCGCTGAAGCAGAGAAAGGATGACAGCATAACCATCCCAACATATATTTCATCACCAGTGACAAAATTGAAAGCCAGCGGAATAGGGACAGTGTCTGGAAGGCTGTTTGTGGGAACAGCTGAGAAAATACCACCCTCTGAGCTCGTAGATACAACAGGTGCTGGAGATAGTTTTATTGGAGCAGTTATTTATGCTGTTTGCACCAACATGCCACCAGAGAAAATGCTCCCTTTCGCTGCTCAAGTGGCGGCTGCCTGCTGTAGAGGTCTGGGAGCCCGAACTAGTCTTCCATACCGCACAGATCCACTCTTGGCACCATTTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

401

Amino Acids

42.74

Weight (kDa)

5.1

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 50 - 276 2.5e-22 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 562
AccB1I GGYRCC 1 cut(s) 1189
AccI GTMKAC 1 cut(s) 15
AciI CCGC 7 cut(s) 122, 139, 183, 701, 954, 1124, 1171
AclWI GGATC 1 cut(s) 1172
AcoI YGGCCR 1 cut(s) 180
AcsI RAATTY 1 cut(s) 695
AcuI CTGAAG 1 cut(s) 902
AcyI GRCGYC 2 cut(s) 153, 559
AfaI GTAC 1 cut(s) 454
AfeI AGCGCT 1 cut(s) 879
AgsI TTSAA 7 cut(s) 45, 242, 312, 510, 747, 757, 946
AhlI ACTAGT 1 cut(s) 1157
AjiI CACGTC 1 cut(s) 859
AjnI CCWGG 3 cut(s) 80, 299, 469
AjuI GAANNNNNNNTTGG 2 cut(s) 907, 939
AloI GAACNNNNNNTCC 2 cut(s) 1147, 1179
AluBI AGCT 6 cut(s) 231, 355, 769, 867, 995, 1018
AluI AGCT 6 cut(s) 231, 355, 769, 867, 995, 1018
Alw21I GWGCWC 2 cut(s) 739, 1020
Alw26I GTCTC 1 cut(s) 193
AlwI GGATC 1 cut(s) 1172
AlwNI CAGNNNCTG 2 cut(s) 971, 1037
Ama87I CYCGRG 1 cut(s) 815
Aor51HI AGCGCT 1 cut(s) 879
AoxI GGCC 2 cut(s) 166, 180
ApeKI GCWGC 4 cut(s) 159, 355, 1112, 1127
ApoI RAATTY 1 cut(s) 695
AseI ATTAAT 1 cut(s) 620
AspLEI GCGC 1 cut(s) 880
AspS9I GGNCC 2 cut(s) 166, 222
AsuC2I CCSGG 1 cut(s) 131
AsuHPI GGTGA 5 cut(s) 49, 57, 402, 806, 921
AsuII TTCGAA 1 cut(s) 873
AvaI CYCGRG 1 cut(s) 815
AvaII GGWCC 1 cut(s) 222
BanI GGYRCC 1 cut(s) 1189
BanII GRGCYC 2 cut(s) 1020, 1153
BbsI GAAGAC 2 cut(s) 90, 1154
Bbv12I GWGCWC 2 cut(s) 739, 1020
BbvI GCAGC 4 cut(s) 171, 342, 1099, 1114
BccI CCATC 4 cut(s) 524, 548, 794, 917
BceAI ACGGC 2 cut(s) 91, 170
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BciT130I CCWGG 3 cut(s) 82, 301, 471
BciVI GTATCC 1 cut(s) 367
BcnI CCSGG 1 cut(s) 131
BcoDI GTCTC 1 cut(s) 193
BcuI ACTAGT 1 cut(s) 1157
BfaI CTAG 1 cut(s) 1158
BfmI CTRYAG 1 cut(s) 1135
BfoI RGCGCY 1 cut(s) 881
BfuI GTATCC 1 cut(s) 367
BisI GCNGC 6 cut(s) 160, 183, 356, 1113, 1125, 1128
BlsI GCNGC 6 cut(s) 161, 184, 357, 1114, 1126, 1129
Bme1390I CCNGG 4 cut(s) 82, 131, 301, 471
Bme18I GGWCC 1 cut(s) 222
BmeT110I CYCGRG 1 cut(s) 815
BmgBI CACGTC 1 cut(s) 859
BmgT120I GGNCC 2 cut(s) 166, 222
BmiI GGNNCC 2 cut(s) 1150, 1191
BmrFI CCNGG 4 cut(s) 82, 131, 301, 471
BpiI GAAGAC 2 cut(s) 90, 1154
BpmI CTGGAG 2 cut(s) 453, 1059
Bpu10I CCTNAGC 1 cut(s) 588
Bpu14I TTCGAA 1 cut(s) 873
BpuEI CTTGAG 2 cut(s) 755, 1101
BpuMI CCSGG 1 cut(s) 131
BsaHI GRCGYC 2 cut(s) 153, 559
BsaJI CCNNGG 4 cut(s) 299, 300, 396, 789
BsaWI WCCGGW 1 cut(s) 490
Bse1I ACTGG 1 cut(s) 932
BseBI CCWGG 3 cut(s) 82, 301, 471
BseDI CCNNGG 4 cut(s) 299, 300, 396, 789
BseGI GGATG 5 cut(s) 499, 535, 658, 901, 909
BseMII CTCAG 6 cut(s) 602, 663, 702, 854, 987, 1005
BseNI ACTGG 1 cut(s) 932
BseRI GAGGAG 5 cut(s) 20, 181, 365, 413, 828
BseXI GCAGC 4 cut(s) 171, 342, 1099, 1114
BsgI GTGCAG 1 cut(s) 548
BshFI GGCC 2 cut(s) 168, 182
BshNI GGYRCC 1 cut(s) 1189
BsiHKAI GWGCWC 2 cut(s) 739, 1020
BsiHKCI CYCGRG 1 cut(s) 815
BsiSI CCGG 3 cut(s) 131, 491, 604
BslFI GGGAC 2 cut(s) 282, 976
BsmAI GTCTC 1 cut(s) 193
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 2 cut(s) 282, 976
BsnI GGCC 2 cut(s) 168, 182
BsoBI CYCGRG 1 cut(s) 815
Bsp119I TTCGAA 1 cut(s) 873
Bsp1286I GDGCHC 3 cut(s) 739, 1020, 1153
Bsp143I GATC 3 cut(s) 216, 655, 1177
BspACI CCGC 7 cut(s) 122, 139, 183, 701, 954, 1124, 1171
BspANI GGCC 2 cut(s) 168, 182
BspCNI CTCAG 6 cut(s) 601, 664, 701, 853, 988, 1006
BspLI GGNNCC 2 cut(s) 1150, 1191
BspPI GGATC 1 cut(s) 1172
BspQI GCTCTTC 1 cut(s) 825
BspT104I TTCGAA 1 cut(s) 873
BspT107I GGYRCC 1 cut(s) 1189
BsrI ACTGG 1 cut(s) 932
BssECI CCNNGG 4 cut(s) 299, 300, 396, 789
BssMI GATC 3 cut(s) 216, 655, 1177
BssNI GRCGYC 2 cut(s) 153, 559
BssT1I CCWWGG 1 cut(s) 789
Bst2UI CCWGG 3 cut(s) 82, 301, 471
Bst4CI ACNGT 2 cut(s) 145, 967
Bst6I CTCTTC 2 cut(s) 40, 825
BstACI GRCGYC 2 cut(s) 153, 559
BstAPI GCANNNNNTGC 1 cut(s) 542
BstBI TTCGAA 1 cut(s) 873
BstC8I GCNNGC 4 cut(s) 360, 705, 952, 1132
BstDEI CTNAG 6 cut(s) 588, 672, 688, 840, 996, 1014
BstF5I GGATG 5 cut(s) 499, 535, 658, 901, 909
BstH2I RGCGCY 1 cut(s) 881
BstHHI GCGC 1 cut(s) 880
BstKTI GATC 3 cut(s) 219, 658, 1180
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 3 cut(s) 216, 655, 1177
BstMWI GCNNNNNNNGC 7 cut(s) 159, 165, 263, 542, 593, 677, 1121
BstNI CCWGG 3 cut(s) 82, 301, 471
BstNSI RCATGY 1 cut(s) 1087
BstSCI CCNGG 4 cut(s) 80, 129, 299, 469
BstSFI CTRYAG 1 cut(s) 1135
BstV1I GCAGC 4 cut(s) 171, 342, 1099, 1114
BstV2I GAAGAC 2 cut(s) 90, 1154
BstX2I RGATCY 1 cut(s) 1177
BstYI RGATCY 1 cut(s) 1177
BsuI GTATCC 1 cut(s) 367
BsuRI GGCC 2 cut(s) 168, 182
BtgZI GCGATG 1 cut(s) 87
BtrI CACGTC 1 cut(s) 859
BtsCI GGATG 5 cut(s) 499, 535, 658, 901, 909
BtsIMutI CAGTG 3 cut(s) 696, 939, 972
Cac8I GCNNGC 4 cut(s) 360, 705, 952, 1132
CaiI CAGNNNCTG 2 cut(s) 971, 1037
CfoI GCGC 1 cut(s) 880
Cfr13I GGNCC 2 cut(s) 166, 222
CseI GACGC 1 cut(s) 142
Csp6I GTAC 1 cut(s) 453
CviAII CATG 3 cut(s) 163, 708, 1084
CviQI GTAC 1 cut(s) 453
DdeI CTNAG 6 cut(s) 588, 672, 688, 840, 996, 1014
DpnI GATC 3 cut(s) 218, 657, 1179
DpnII GATC 3 cut(s) 216, 655, 1177
EaeI YGGCCR 1 cut(s) 180
Eam1104I CTCTTC 2 cut(s) 40, 825
EarI CTCTTC 2 cut(s) 40, 825
EciI GGCGGA 1 cut(s) 111
Ecl136II GAGCTC 1 cut(s) 1018
Eco130I CCWWGG 1 cut(s) 789
Eco24I GRGCYC 2 cut(s) 1020, 1153
Eco32I GATATC 1 cut(s) 476
Eco47I GGWCC 1 cut(s) 222
Eco47III AGCGCT 1 cut(s) 879
Eco53kI GAGCTC 1 cut(s) 1018
Eco57I CTGAAG 1 cut(s) 902
Eco88I CYCGRG 1 cut(s) 815
EcoICRI GAGCTC 1 cut(s) 1018
EcoRII CCWGG 3 cut(s) 80, 299, 469
EcoRV GATATC 1 cut(s) 476
EcoT14I CCWWGG 1 cut(s) 789
EcoT38I GRGCYC 2 cut(s) 1020, 1153
ErhI CCWWGG 1 cut(s) 789
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 3 cut(s) 166, 711, 1087
FalI AAGNNNNNCTT 2 cut(s) 529, 561
FaqI GGGAC 2 cut(s) 282, 976
FatI CATG 3 cut(s) 162, 707, 1083
FauNDI CATATG 1 cut(s) 683
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 6 cut(s) 160, 183, 356, 1113, 1125, 1128
FokI GGATG 5 cut(s) 506, 542, 665, 896, 908
FriOI GRGCYC 2 cut(s) 1020, 1153
Fsp4HI GCNGC 6 cut(s) 160, 183, 356, 1113, 1125, 1128
FspBI CTAG 1 cut(s) 1158
GlaI GCGC 1 cut(s) 879
GluI GCNGC 6 cut(s) 160, 183, 356, 1113, 1125, 1128
GsuI CTGGAG 2 cut(s) 453, 1059
HaeII RGCGCY 1 cut(s) 881
HaeIII GGCC 2 cut(s) 168, 182
HapII CCGG 3 cut(s) 131, 491, 604
HgaI GACGC 1 cut(s) 142
HhaI GCGC 1 cut(s) 880
Hin1I GRCGYC 2 cut(s) 153, 559
Hin1II CATG 3 cut(s) 166, 711, 1087
Hin6I GCGC 1 cut(s) 878
HinP1I GCGC 1 cut(s) 878
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HindIII AAGCTT 1 cut(s) 865
HinfI GANTC 2 cut(s) 113, 295
HpaII CCGG 3 cut(s) 131, 491, 604
HphI GGTGA 5 cut(s) 49, 57, 402, 806, 921
Hpy166II GTNNAC 2 cut(s) 16, 433
Hpy188I TCNGA 5 cut(s) 112, 322, 732, 843, 1015
Hpy188III TCNNGA 4 cut(s) 220, 590, 817, 972
Hpy8I GTNNAC 2 cut(s) 16, 433
Hpy99I CGWCG 3 cut(s) 158, 212, 368
HpyAV CCTTC 3 cut(s) 516, 638, 969
HpyCH4III ACNGT 2 cut(s) 145, 967
HpyCH4IV ACGT 2 cut(s) 559, 858
HpyCH4V TGCA 5 cut(s) 358, 524, 565, 779, 1077
HpyF10VI GCNNNNNNNGC 7 cut(s) 159, 165, 263, 542, 593, 677, 1121
HpyF3I CTNAG 6 cut(s) 588, 672, 688, 840, 996, 1014
HpySE526I ACGT 2 cut(s) 559, 858
Hsp92I GRCGYC 2 cut(s) 153, 559
Hsp92II CATG 3 cut(s) 166, 711, 1087
HspAI GCGC 1 cut(s) 878
Kzo9I GATC 3 cut(s) 216, 655, 1177
LguI GCTCTTC 1 cut(s) 825
LmnI GCTCC 6 cut(s) 352, 533, 606, 1055, 1107, 1148
Lsp1109I GCAGC 4 cut(s) 171, 342, 1099, 1114
MaeI CTAG 1 cut(s) 1158
MaeII ACGT 2 cut(s) 559, 858
MaeIII GTNAC 3 cut(s) 63, 784, 934
MalI GATC 3 cut(s) 218, 657, 1179
MboI GATC 3 cut(s) 216, 655, 1177
MboII GAAGA 9 cut(s) 27, 30, 33, 90, 650, 809, 842, 860, 1154
MfeI CAATTG 1 cut(s) 780
MflI RGATCY 1 cut(s) 1177
MhlI GDGCHC 3 cut(s) 739, 1020, 1153
MluCI AATT 6 cut(s) 406, 621, 695, 758, 780, 941
MlyI GAGTC 1 cut(s) 304
MmeI TCCRAC 1 cut(s) 630
MseI TTAA 3 cut(s) 233, 620, 809
MslI CAYNNNNRTG 2 cut(s) 1082, 1198
MspA1I CMGCKG 2 cut(s) 954, 995
MspI CCGG 3 cut(s) 131, 491, 604
MspR9I CCNGG 4 cut(s) 82, 131, 301, 471
MunI CAATTG 1 cut(s) 780
MvaI CCWGG 3 cut(s) 82, 301, 471
MwoI GCNNNNNNNGC 7 cut(s) 159, 165, 263, 542, 593, 677, 1121
NciI CCSGG 1 cut(s) 131
NdeI CATATG 1 cut(s) 683
NdeII GATC 3 cut(s) 216, 655, 1177
NlaIII CATG 3 cut(s) 166, 711, 1087
NlaIV GGNNCC 2 cut(s) 1150, 1191
NmeAIII GCCGAG 1 cut(s) 421
NmuCI GTSAC 3 cut(s) 63, 784, 934
NspI RCATGY 1 cut(s) 1087
NspV TTCGAA 1 cut(s) 873
PaeR7I CTCGAG 1 cut(s) 815
PasI CCCWGGG 1 cut(s) 300
PciSI GCTCTTC 1 cut(s) 825
PfeI GAWTC 1 cut(s) 113
PflFI GACNNNGTC 1 cut(s) 967
PfoI TCCNGGA 2 cut(s) 129, 469
PkrI GCNGC 6 cut(s) 161, 184, 357, 1114, 1126, 1129
PleI GAGTC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 303
PshBI ATTAAT 1 cut(s) 620
Psp124BI GAGCTC 1 cut(s) 1020
Psp6I CCWGG 3 cut(s) 80, 299, 469
PspGI CCWGG 3 cut(s) 80, 299, 469
PspN4I GGNNCC 2 cut(s) 1150, 1191
PspPI GGNCC 2 cut(s) 166, 222
PstNI CAGNNNCTG 2 cut(s) 971, 1037
PsuI RGATCY 1 cut(s) 1177
PsyI GACNNNGTC 1 cut(s) 967
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 1 cut(s) 454
RsaNI GTAC 1 cut(s) 453
RseI CAYNNNNRTG 2 cut(s) 1082, 1198
SacI GAGCTC 1 cut(s) 1020
SapI GCTCTTC 1 cut(s) 825
SaqAI TTAA 3 cut(s) 233, 620, 809
SatI GCNGC 6 cut(s) 160, 183, 356, 1113, 1125, 1128
Sau3AI GATC 3 cut(s) 216, 655, 1177
Sau96I GGNCC 2 cut(s) 166, 222
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 4 cut(s) 82, 131, 301, 471
SduI GDGCHC 3 cut(s) 739, 1020, 1153
SfcI CTRYAG 1 cut(s) 1135
Sfr274I CTCGAG 1 cut(s) 815
SfuI TTCGAA 1 cut(s) 873
SinI GGWCC 1 cut(s) 222
SlaI CTCGAG 1 cut(s) 815
SmiMI CAYNNNNRTG 2 cut(s) 1082, 1198
SmlI CTYRAG 3 cut(s) 770, 815, 1116
SmoI CTYRAG 3 cut(s) 770, 815, 1116
SpeI ACTAGT 1 cut(s) 1157
Sse9I AATT 6 cut(s) 406, 621, 695, 758, 780, 941
SsiI CCGC 7 cut(s) 122, 139, 183, 701, 954, 1124, 1171
SspMI CTAG 1 cut(s) 1158
SstI GAGCTC 1 cut(s) 1020
StyD4I CCNGG 4 cut(s) 80, 129, 299, 469
StyI CCWWGG 1 cut(s) 789
TaaI ACNGT 2 cut(s) 145, 967
TaiI ACGT 2 cut(s) 562, 861
TaqI TCGA 3 cut(s) 171, 816, 873
TasI AATT 6 cut(s) 406, 621, 695, 758, 780, 941
TauI GCSGC 2 cut(s) 185, 1127
TfiI GAWTC 1 cut(s) 113
Tru1I TTAA 3 cut(s) 233, 620, 809
Tru9I TTAA 3 cut(s) 233, 620, 809
TscAI CASTG 3 cut(s) 696, 939, 972
TseFI GTSAC 3 cut(s) 63, 784, 934
TseI GCWGC 4 cut(s) 159, 355, 1112, 1127
Tsp45I GTSAC 3 cut(s) 63, 784, 934
TspDTI ATGAA 3 cut(s) 90, 452, 915
TspGWI ACGGA 1 cut(s) 860
TspRI CASTG 3 cut(s) 696, 939, 972
Tth111I GACNNNGTC 1 cut(s) 967
VpaK11BI GGWCC 1 cut(s) 222
VspI ATTAAT 1 cut(s) 620
XapI RAATTY 1 cut(s) 695
XceI RCATGY 1 cut(s) 1087
XhoI CTCGAG 1 cut(s) 815
XmiI GTMKAC 1 cut(s) 15
XspI CTAG 1 cut(s) 1158
ZraI GACGTC 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.