Rh6BG291600

Belongs to the carbohydrate kinase PfkB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0004679
Physical Location & Seq
Reverse (-)
51719788 .. 51724958
5171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG291600.1

Sequence Viewer

Length: 819 bp
ATGGGGCTGGATTTCTTGGCCGTCGTGTCCTCTTATCCTAAGCCGGACGATAAGATCAGGACCACCAGCTTGAAGGTTCAAGGAGGTGGAAACGCCAGTAATGCTTTAACTTGTGTGGCTCGTTTGGGGCTAAGTCCCAGGGTCATTTCCAAGGTTGCGGATGATACACAAGGCAGGGGTATAATGGAGGAGCTACAAGCCGAAGGCGTAGATACTTCGTTTGTTGTGGTTGCTGAGGAGGGTAATTCACCATTTACCTACATTATTGTTGACAATGAAACGAATACTCGTACGTGTATTCATACTGAAGGATATCCTCCTATGATACCGGATGACCTTTCACCTTCAAGTTTATCGTCTGCACTGGATGGAGCAAGACTTGCTTATTTTGATGGACGTCTACATGAAACTGCTTTACTTGTTGCACAGGAGGCTACTCGCCGGAACATACCTATATTAATTGAGGCTGAAAGGATAAGAGAAGGGTTGGATGATCTTCTAAACTTGTCTGAGTATGCCATATGCTCAGCAAAATTTCCAAAGGCATGGACAGGAGCAGAAACTTTTCCAAGTGCTCTTGTTTCAATGCTTTTGAAATTGCCAAAGCTCAAATTTTCAATTGTGACCTTGGGTGAAGATGGATGCATAATGGTTGAGAGAAATCTAGAAGAGGCTCCTCAGACGGAAGAAATTGACGTGGACAGCTTATTCGAAGCGCTGAAGCAGAGAAAGGATGACAGCTTAACCAAACCAACATATGTTTCATCTGTAAGCCACTTTCAAATTACATTAAAAGTATACTCCTTTTGCTTTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

272

Amino Acids

29.89

Weight (kDa)

4.74

Isoelectric Point (pI)

31.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 10 - 221 5.1e-22 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 400
AccI GTMKAC 2 cut(s) 400, 798
AciI CCGC 1 cut(s) 158
AcoI YGGCCR 1 cut(s) 18
AcsI RAATTY 2 cut(s) 533, 611
AcuI CTGAAG 2 cut(s) 327, 740
AcyI GRCGYC 1 cut(s) 397
AfaI GTAC 1 cut(s) 292
AfeI AGCGCT 1 cut(s) 717
AflIII ACRYGT 1 cut(s) 293
AgsI TTSAA 7 cut(s) 73, 80, 348, 585, 595, 618, 782
AjiI CACGTC 1 cut(s) 697
AjnI CCWGG 1 cut(s) 137
AjuI GAANNNNNNNTTGG 2 cut(s) 745, 777
AluBI AGCT 5 cut(s) 69, 193, 607, 705, 741
AluI AGCT 5 cut(s) 69, 193, 607, 705, 741
Alw21I GWGCWC 1 cut(s) 577
Aor51HI AGCGCT 1 cut(s) 717
AoxI GGCC 1 cut(s) 18
ApoI RAATTY 2 cut(s) 533, 611
ArsI GACNNNNNNTTYG 2 cut(s) 692, 724
AseI ATTAAT 1 cut(s) 458
Asp700I GAANNNNTTC 1 cut(s) 564
AspLEI GCGC 1 cut(s) 718
AspS9I GGNCC 1 cut(s) 60
AsuHPI GGTGA 3 cut(s) 240, 333, 644
AsuII TTCGAA 1 cut(s) 711
AvaII GGWCC 1 cut(s) 60
Bbv12I GWGCWC 1 cut(s) 577
BbvCI CCTCAGC 1 cut(s) 234
BccI CCATC 3 cut(s) 362, 386, 632
BceAI ACGGC 1 cut(s) 5
BciT130I CCWGG 1 cut(s) 139
BfaI CTAG 1 cut(s) 665
BfoI RGCGCY 1 cut(s) 719
BlpI GCTNAGC 1 cut(s) 526
Bme1390I CCNGG 1 cut(s) 139
Bme18I GGWCC 1 cut(s) 60
BmgBI CACGTC 1 cut(s) 697
BmgT120I GGNCC 1 cut(s) 60
BmiI GGNNCC 1 cut(s) 675
BmrFI CCNGG 1 cut(s) 139
BmsI GCATC 1 cut(s) 632
Bpu10I CCTNAGC 2 cut(s) 39, 234
Bpu1102I GCTNAGC 1 cut(s) 526
Bpu14I TTCGAA 1 cut(s) 711
BsaAI YACGTR 1 cut(s) 294
BsaHI GRCGYC 1 cut(s) 397
BsaJI CCNNGG 4 cut(s) 137, 138, 150, 627
BsaWI WCCGGW 1 cut(s) 328
Bse1I ACTGG 2 cut(s) 96, 369
BseBI CCWGG 1 cut(s) 139
BseDI CCNNGG 4 cut(s) 137, 138, 150, 627
BseGI GGATG 6 cut(s) 166, 337, 373, 496, 647, 739
BseMII CTCAG 4 cut(s) 225, 501, 540, 692
BseNI ACTGG 2 cut(s) 96, 369
BseRI GAGGAG 3 cut(s) 203, 251, 666
BsgI GTGCAG 1 cut(s) 345
BshFI GGCC 1 cut(s) 20
BsiHKAI GWGCWC 1 cut(s) 577
BsiSI CCGG 3 cut(s) 44, 329, 442
BsiWI CGTACG 1 cut(s) 290
BslFI GGGAC 1 cut(s) 120
BsmFI GGGAC 1 cut(s) 120
BsnI GGCC 1 cut(s) 20
Bsp119I TTCGAA 1 cut(s) 711
Bsp1286I GDGCHC 1 cut(s) 577
Bsp143I GATC 2 cut(s) 54, 493
Bsp1720I GCTNAGC 1 cut(s) 526
BspACI CCGC 1 cut(s) 158
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 4 cut(s) 226, 502, 539, 691
BspLI GGNNCC 1 cut(s) 675
BspT104I TTCGAA 1 cut(s) 711
BsrI ACTGG 2 cut(s) 96, 369
BssECI CCNNGG 4 cut(s) 137, 138, 150, 627
BssMI GATC 2 cut(s) 54, 493
BssNAI GTATAC 1 cut(s) 799
BssNI GRCGYC 1 cut(s) 397
BssT1I CCWWGG 2 cut(s) 150, 627
Bst1107I GTATAC 1 cut(s) 799
Bst2UI CCWGG 1 cut(s) 139
Bst6I CTCTTC 1 cut(s) 663
BstACI GRCGYC 1 cut(s) 397
BstAPI GCANNNNNTGC 1 cut(s) 380
BstBAI YACGTR 1 cut(s) 294
BstBI TTCGAA 1 cut(s) 711
BstDEI CTNAG 6 cut(s) 39, 131, 234, 510, 526, 678
BstF5I GGATG 6 cut(s) 166, 337, 373, 496, 647, 739
BstH2I RGCGCY 1 cut(s) 719
BstHHI GCGC 1 cut(s) 718
BstKTI GATC 2 cut(s) 57, 496
BstMBI GATC 2 cut(s) 54, 493
BstMWI GCNNNNNNNGC 3 cut(s) 101, 380, 431
BstNI CCWGG 1 cut(s) 139
BstSCI CCNGG 1 cut(s) 137
BstXI CCANNNNNNTGG 1 cut(s) 546
BstZ17I GTATAC 1 cut(s) 799
BsuRI GGCC 1 cut(s) 20
BtrI CACGTC 1 cut(s) 697
BtsCI GGATG 6 cut(s) 166, 337, 373, 496, 647, 739
BtsIMutI CAGTG 1 cut(s) 362
CfoI GCGC 1 cut(s) 718
Cfr13I GGNCC 1 cut(s) 60
Csp6I GTAC 1 cut(s) 291
CviAII CATG 2 cut(s) 404, 546
CviQI GTAC 1 cut(s) 291
DdeI CTNAG 6 cut(s) 39, 131, 234, 510, 526, 678
DpnI GATC 2 cut(s) 56, 495
DpnII GATC 2 cut(s) 54, 493
EaeI YGGCCR 1 cut(s) 18
Eam1104I CTCTTC 1 cut(s) 663
EarI CTCTTC 1 cut(s) 663
Eco130I CCWWGG 2 cut(s) 150, 627
Eco32I GATATC 1 cut(s) 314
Eco47I GGWCC 1 cut(s) 60
Eco47III AGCGCT 1 cut(s) 717
Eco57I CTGAAG 2 cut(s) 327, 740
EcoRII CCWGG 1 cut(s) 137
EcoRV GATATC 1 cut(s) 314
EcoT14I CCWWGG 2 cut(s) 150, 627
EcoT22I ATGCAT 1 cut(s) 647
ErhI CCWWGG 2 cut(s) 150, 627
FaeI CATG 2 cut(s) 407, 549
FalI AAGNNNNNCTT 2 cut(s) 367, 399
FaqI GGGAC 1 cut(s) 120
FatI CATG 2 cut(s) 403, 545
FauNDI CATATG 2 cut(s) 521, 757
FblI GTMKAC 2 cut(s) 400, 798
FokI GGATG 6 cut(s) 173, 344, 380, 503, 654, 746
FspBI CTAG 1 cut(s) 665
GlaI GCGC 1 cut(s) 717
HaeII RGCGCY 1 cut(s) 719
HaeIII GGCC 1 cut(s) 20
HapII CCGG 3 cut(s) 44, 329, 442
HhaI GCGC 1 cut(s) 718
Hin1I GRCGYC 1 cut(s) 397
Hin1II CATG 2 cut(s) 407, 549
Hin6I GCGC 1 cut(s) 716
HinP1I GCGC 1 cut(s) 716
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HpaII CCGG 3 cut(s) 44, 329, 442
HphI GGTGA 3 cut(s) 240, 333, 644
Hpy166II GTNNAC 4 cut(s) 271, 401, 700, 799
Hpy188I TCNGA 2 cut(s) 511, 681
Hpy188III TCNNGA 2 cut(s) 58, 665
Hpy8I GTNNAC 4 cut(s) 271, 401, 700, 799
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 5 cut(s) 67, 197, 302, 354, 476
HpyCH4IV ACGT 3 cut(s) 293, 397, 696
HpyCH4V TGCA 3 cut(s) 362, 425, 645
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 380, 431
HpyF3I CTNAG 6 cut(s) 39, 131, 234, 510, 526, 678
HpySE526I ACGT 3 cut(s) 293, 397, 696
Hsp92I GRCGYC 1 cut(s) 397
Hsp92II CATG 2 cut(s) 407, 549
HspAI GCGC 1 cut(s) 716
Kzo9I GATC 2 cut(s) 54, 493
LmnI GCTCC 4 cut(s) 190, 371, 554, 679
LweI GCATC 1 cut(s) 632
MaeI CTAG 1 cut(s) 665
MaeII ACGT 3 cut(s) 293, 397, 696
MaeIII GTNAC 1 cut(s) 622
MalI GATC 2 cut(s) 56, 495
MboI GATC 2 cut(s) 54, 493
MboII GAAGA 4 cut(s) 488, 647, 680, 698
MfeI CAATTG 1 cut(s) 618
MhlI GDGCHC 1 cut(s) 577
MluCI AATT 8 cut(s) 244, 459, 533, 596, 611, 618, 690, 783
MmeI TCCRAC 1 cut(s) 468
Mph1103I ATGCAT 1 cut(s) 647
MroXI GAANNNNTTC 1 cut(s) 564
MseI TTAA 4 cut(s) 107, 458, 743, 791
MspI CCGG 3 cut(s) 44, 329, 442
MspR9I CCNGG 1 cut(s) 139
MunI CAATTG 1 cut(s) 618
MvaI CCWGG 1 cut(s) 139
MwoI GCNNNNNNNGC 3 cut(s) 101, 380, 431
NdeI CATATG 2 cut(s) 521, 757
NdeII GATC 2 cut(s) 54, 493
NlaIII CATG 2 cut(s) 407, 549
NlaIV GGNNCC 1 cut(s) 675
NmuCI GTSAC 1 cut(s) 622
NsiI ATGCAT 1 cut(s) 647
NspV TTCGAA 1 cut(s) 711
PasI CCCWGGG 1 cut(s) 138
PdmI GAANNNNTTC 1 cut(s) 564
Pfl23II CGTACG 1 cut(s) 290
Ppu21I YACGTR 1 cut(s) 294
PshBI ATTAAT 1 cut(s) 458
Psp6I CCWGG 1 cut(s) 137
PspGI CCWGG 1 cut(s) 137
PspLI CGTACG 1 cut(s) 290
PspN4I GGNNCC 1 cut(s) 675
PspPI GGNCC 1 cut(s) 60
RsaI GTAC 1 cut(s) 292
RsaNI GTAC 1 cut(s) 291
SaqAI TTAA 4 cut(s) 107, 458, 743, 791
Sau3AI GATC 2 cut(s) 54, 493
Sau96I GGNCC 1 cut(s) 60
ScrFI CCNGG 1 cut(s) 139
SduI GDGCHC 1 cut(s) 577
SfaNI GCATC 1 cut(s) 632
SfuI TTCGAA 1 cut(s) 711
SinI GGWCC 1 cut(s) 60
Sse9I AATT 8 cut(s) 244, 459, 533, 596, 611, 618, 690, 783
SsiI CCGC 1 cut(s) 158
SspMI CTAG 1 cut(s) 665
StyD4I CCNGG 1 cut(s) 137
StyI CCWWGG 2 cut(s) 150, 627
TaiI ACGT 3 cut(s) 296, 400, 699
TaqI TCGA 1 cut(s) 711
TasI AATT 8 cut(s) 244, 459, 533, 596, 611, 618, 690, 783
Tru1I TTAA 4 cut(s) 107, 458, 743, 791
Tru9I TTAA 4 cut(s) 107, 458, 743, 791
TscAI CASTG 1 cut(s) 369
TseFI GTSAC 1 cut(s) 622
Tsp45I GTSAC 1 cut(s) 622
TspDTI ATGAA 4 cut(s) 290, 291, 420, 753
TspGWI ACGGA 1 cut(s) 698
TspRI CASTG 1 cut(s) 369
VpaK11BI GGWCC 1 cut(s) 60
VspI ATTAAT 1 cut(s) 458
XapI RAATTY 2 cut(s) 533, 611
XbaI TCTAGA 1 cut(s) 664
XmiI GTMKAC 2 cut(s) 400, 798
XmnI GAANNNNTTC 1 cut(s) 564
XspI CTAG 1 cut(s) 665
ZraI GACGTC 1 cut(s) 398
Zsp2I ATGCAT 1 cut(s) 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.