Rh6AG289700

Belongs to the carbohydrate kinase PfkB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0004679
Physical Location & Seq
Reverse (-)
48689061 .. 48694311
5251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG289700.1

Sequence Viewer

Length: 1050 bp
ATGCTGTCTCTTGTTTACACGACCCCAAAAGCCAATTCCACCTCTCCTCTCGTCAGCTCACCTCACTATTCTTCTCCTCCTCCTCCTTTTCACACTTCACCGTCAAAGACGCACCACAGCCTATCTCTACCTTCGTCACCACGTCGCCCAACCAGGTTCAGAATGTCGTCCTCCGATTCGCTTCCCCCTCCCCCTCTCCCGGAAAACCGGACCGTGCTCGGTGTGGGCAGCATGGGGCTGGATTTCTTGGCCGTCGTGTCCTCTTATCCTAAGCCGGACGATAAGATCAGGACCACCAGCTTGAAGGTTCAAGGAGGTGGAAATGCCAGTAATGCTTTAACTTGTGTGGCTCGCTTGGGGCTAAGTCCCAGGGTCATTTCCAAGGTTGCGGATGATACACAAGGCAGGGGTATACTGGAGGAGCTACAAGCCGAAGGCGTAGATACTTCGTTTGCTGTGGTTGCTGAGGAGGGTAATTCACCATTTACCTACATTATTGTTGACAATGAAACGAATACTCGTACGTGTATTCATACTGAAGGATATCCTCCTATGATACCGGATGACCTTTCACCTTCAAGTTTATCGTCTGCACTGGATGGAACAAGACTTGCTTATTTTGATGGACGTCTACATGAAACTGCTTTACTTGTTGCACAGGAGGCTACTCGCCGGAACATACCTATATTAATTGAGGCTGAAAGAATAAGAGAAGGGTTGGATGATCTTCTAAACTTGTCTGAGTATGCCATATGCTCAGCAAAATTTCCAAAGGCATGGACAGGAGCAGAAACTTTTCCAAGTGCTCTTGTTTCAATGCTTTTGAAATTGCCAAAGCTCAAATTTGCAATTGTGACCTTGGGTGAAGATGGATGCATAATGCTTGAGAGAAATCTAGAAGAGGCTCCTCAGACGGAAGAAATTGACGTGGACAGCTTATTCGAAGCGCTGAAGCAGAGAAAGGATGACAGCTTAACCAAACCAACATATATTTCATCTGTAAGCCACTTTCAAATTACATTAAAAGTATACTCCTTTTGCTTTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

349

Amino Acids

38.14

Weight (kDa)

5.46

Isoelectric Point (pI)

47.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 73 - 298 2.3e-21 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 631
AccI GTMKAC 3 cut(s) 412, 631, 1029
AciI CCGC 1 cut(s) 389
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 2 cut(s) 764, 842
AcuI CTGAAG 2 cut(s) 558, 971
AcyI GRCGYC 1 cut(s) 628
AfaI GTAC 1 cut(s) 523
AfeI AGCGCT 1 cut(s) 948
AfiI CCNNNNNNNGG 1 cut(s) 199
AflIII ACRYGT 1 cut(s) 524
AgsI TTSAA 6 cut(s) 304, 311, 579, 816, 826, 1013
AjiI CACGTC 2 cut(s) 143, 928
AjnI CCWGG 2 cut(s) 152, 368
AjuI GAANNNNNNNTTGG 4 cut(s) 19, 51, 976, 1008
AluBI AGCT 6 cut(s) 57, 300, 424, 838, 936, 972
AluI AGCT 6 cut(s) 57, 300, 424, 838, 936, 972
Alw21I GWGCWC 2 cut(s) 219, 808
Alw26I GTCTC 1 cut(s) 12
Aor51HI AGCGCT 1 cut(s) 948
AoxI GGCC 1 cut(s) 249
ApeKI GCWGC 1 cut(s) 228
ApoI RAATTY 2 cut(s) 764, 842
ArsI GACNNNNNNTTYG 2 cut(s) 923, 955
AseI ATTAAT 1 cut(s) 689
Asp700I GAANNNNTTC 1 cut(s) 795
AspLEI GCGC 1 cut(s) 949
AspS9I GGNCC 2 cut(s) 210, 291
AsuC2I CCSGG 1 cut(s) 200
AsuHPI GGTGA 6 cut(s) 51, 90, 129, 471, 564, 875
AsuII TTCGAA 1 cut(s) 942
AvaII GGWCC 2 cut(s) 210, 291
Bbv12I GWGCWC 2 cut(s) 219, 808
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 1 cut(s) 240
BccI CCATC 3 cut(s) 593, 617, 863
BceAI ACGGC 1 cut(s) 236
BciT130I CCWGG 2 cut(s) 154, 370
BcnI CCSGG 1 cut(s) 200
BcoDI GTCTC 1 cut(s) 12
BfaI CTAG 1 cut(s) 896
BfoI RGCGCY 1 cut(s) 950
BisI GCNGC 1 cut(s) 229
BlpI GCTNAGC 1 cut(s) 757
BlsI GCNGC 1 cut(s) 230
Bme1390I CCNGG 3 cut(s) 154, 200, 370
Bme18I GGWCC 2 cut(s) 210, 291
BmgBI CACGTC 2 cut(s) 143, 928
BmgT120I GGNCC 2 cut(s) 210, 291
BmiI GGNNCC 1 cut(s) 906
BmrFI CCNGG 3 cut(s) 154, 200, 370
BmsI GCATC 1 cut(s) 863
BpmI CTGGAG 1 cut(s) 437
Bpu10I CCTNAGC 2 cut(s) 270, 465
Bpu1102I GCTNAGC 1 cut(s) 757
Bpu14I TTCGAA 1 cut(s) 942
BpuEI CTTGAG 1 cut(s) 905
BpuMI CCSGG 1 cut(s) 200
BsaAI YACGTR 1 cut(s) 525
BsaHI GRCGYC 1 cut(s) 628
BsaJI CCNNGG 4 cut(s) 368, 369, 381, 858
BsaWI WCCGGW 2 cut(s) 207, 559
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse1I ACTGG 3 cut(s) 327, 420, 600
BseBI CCWGG 2 cut(s) 154, 370
BseDI CCNNGG 4 cut(s) 368, 369, 381, 858
BseGI GGATG 6 cut(s) 397, 568, 604, 727, 878, 970
BseLI CCNNNNNNNGG 1 cut(s) 199
BseMII CTCAG 4 cut(s) 456, 732, 771, 923
BseNI ACTGG 3 cut(s) 327, 420, 600
BseRI GAGGAG 7 cut(s) 36, 66, 69, 72, 434, 482, 897
BseXI GCAGC 1 cut(s) 240
BsgI GTGCAG 1 cut(s) 576
BshFI GGCC 1 cut(s) 251
BsiHKAI GWGCWC 2 cut(s) 219, 808
BsiSI CCGG 5 cut(s) 200, 208, 275, 560, 673
BsiWI CGTACG 1 cut(s) 521
BslFI GGGAC 1 cut(s) 351
BslI CCNNNNNNNGG 1 cut(s) 199
BsmAI GTCTC 1 cut(s) 12
BsmFI GGGAC 1 cut(s) 351
BsnI GGCC 1 cut(s) 251
Bsp119I TTCGAA 1 cut(s) 942
Bsp1286I GDGCHC 2 cut(s) 219, 808
Bsp143I GATC 2 cut(s) 285, 724
Bsp1720I GCTNAGC 1 cut(s) 757
BspACI CCGC 1 cut(s) 389
BspANI GGCC 1 cut(s) 251
BspCNI CTCAG 4 cut(s) 457, 733, 770, 922
BspLI GGNNCC 1 cut(s) 906
BspT104I TTCGAA 1 cut(s) 942
BsrI ACTGG 3 cut(s) 327, 420, 600
BssECI CCNNGG 4 cut(s) 368, 369, 381, 858
BssMI GATC 2 cut(s) 285, 724
BssNAI GTATAC 2 cut(s) 413, 1030
BssNI GRCGYC 1 cut(s) 628
BssT1I CCWWGG 2 cut(s) 381, 858
Bst1107I GTATAC 2 cut(s) 413, 1030
Bst2UI CCWGG 2 cut(s) 154, 370
Bst4CI ACNGT 2 cut(s) 102, 214
Bst6I CTCTTC 1 cut(s) 894
BstACI GRCGYC 1 cut(s) 628
BstBAI YACGTR 1 cut(s) 525
BstBI TTCGAA 1 cut(s) 942
BstC8I GCNNGC 1 cut(s) 352
BstDEI CTNAG 6 cut(s) 270, 362, 465, 741, 757, 909
BstF5I GGATG 6 cut(s) 397, 568, 604, 727, 878, 970
BstH2I RGCGCY 1 cut(s) 950
BstHHI GCGC 1 cut(s) 949
BstKTI GATC 2 cut(s) 288, 727
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 2 cut(s) 285, 724
BstMWI GCNNNNNNNGC 3 cut(s) 332, 461, 662
BstNI CCWGG 2 cut(s) 154, 370
BstSCI CCNGG 3 cut(s) 152, 198, 368
BstV1I GCAGC 1 cut(s) 240
BstXI CCANNNNNNTGG 1 cut(s) 777
BstZ17I GTATAC 2 cut(s) 413, 1030
BsuRI GGCC 1 cut(s) 251
BtrI CACGTC 2 cut(s) 143, 928
BtsCI GGATG 6 cut(s) 397, 568, 604, 727, 878, 970
BtsIMutI CAGTG 1 cut(s) 593
Cac8I GCNNGC 1 cut(s) 352
CfoI GCGC 1 cut(s) 949
Cfr13I GGNCC 2 cut(s) 210, 291
CpoI CGGWCCG 1 cut(s) 210
CseI GACGC 1 cut(s) 118
CsiI ACCWGGT 1 cut(s) 152
Csp6I GTAC 1 cut(s) 522
CspI CGGWCCG 1 cut(s) 210
CviAII CATG 3 cut(s) 232, 635, 777
CviQI GTAC 1 cut(s) 522
DdeI CTNAG 6 cut(s) 270, 362, 465, 741, 757, 909
DpnI GATC 2 cut(s) 287, 726
DpnII GATC 2 cut(s) 285, 724
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 894
EarI CTCTTC 1 cut(s) 894
Eco130I CCWWGG 2 cut(s) 381, 858
Eco32I GATATC 1 cut(s) 545
Eco47I GGWCC 2 cut(s) 210, 291
Eco47III AGCGCT 1 cut(s) 948
Eco57I CTGAAG 2 cut(s) 558, 971
EcoRII CCWGG 2 cut(s) 152, 368
EcoRV GATATC 1 cut(s) 545
EcoT14I CCWWGG 2 cut(s) 381, 858
EcoT22I ATGCAT 1 cut(s) 878
ErhI CCWWGG 2 cut(s) 381, 858
FaeI CATG 3 cut(s) 235, 638, 780
FalI AAGNNNNNCTT 2 cut(s) 598, 630
FaqI GGGAC 1 cut(s) 351
FatI CATG 3 cut(s) 231, 634, 776
FauNDI CATATG 1 cut(s) 752
FblI GTMKAC 3 cut(s) 412, 631, 1029
Fnu4HI GCNGC 1 cut(s) 229
FokI GGATG 6 cut(s) 404, 575, 611, 734, 885, 977
Fsp4HI GCNGC 1 cut(s) 229
FspBI CTAG 1 cut(s) 896
GlaI GCGC 1 cut(s) 948
GluI GCNGC 1 cut(s) 229
GsuI CTGGAG 1 cut(s) 437
HaeII RGCGCY 1 cut(s) 950
HaeIII GGCC 1 cut(s) 251
HapII CCGG 5 cut(s) 200, 208, 275, 560, 673
HgaI GACGC 1 cut(s) 118
HhaI GCGC 1 cut(s) 949
Hin1I GRCGYC 1 cut(s) 628
Hin1II CATG 3 cut(s) 235, 638, 780
Hin6I GCGC 1 cut(s) 947
HinP1I GCGC 1 cut(s) 947
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HinfI GANTC 1 cut(s) 176
HpaII CCGG 5 cut(s) 200, 208, 275, 560, 673
HphI GGTGA 6 cut(s) 51, 90, 129, 471, 564, 875
Hpy166II GTNNAC 6 cut(s) 16, 413, 502, 632, 931, 1030
Hpy188I TCNGA 4 cut(s) 161, 175, 742, 912
Hpy188III TCNNGA 2 cut(s) 289, 896
Hpy8I GTNNAC 6 cut(s) 16, 413, 502, 632, 931, 1030
Hpy99I CGWCG 2 cut(s) 147, 257
HpyAV CCTTC 6 cut(s) 141, 298, 428, 533, 585, 707
HpyCH4III ACNGT 2 cut(s) 102, 214
HpyCH4IV ACGT 4 cut(s) 142, 524, 628, 927
HpyCH4V TGCA 4 cut(s) 593, 656, 848, 876
HpyF10VI GCNNNNNNNGC 3 cut(s) 332, 461, 662
HpyF3I CTNAG 6 cut(s) 270, 362, 465, 741, 757, 909
HpySE526I ACGT 4 cut(s) 142, 524, 628, 927
Hsp92I GRCGYC 1 cut(s) 628
Hsp92II CATG 3 cut(s) 235, 638, 780
HspAI GCGC 1 cut(s) 947
Kzo9I GATC 2 cut(s) 285, 724
LmnI GCTCC 3 cut(s) 421, 785, 910
Lsp1109I GCAGC 1 cut(s) 240
LweI GCATC 1 cut(s) 863
MabI ACCWGGT 1 cut(s) 152
MaeI CTAG 1 cut(s) 896
MaeII ACGT 4 cut(s) 142, 524, 628, 927
MaeIII GTNAC 2 cut(s) 135, 853
MalI GATC 2 cut(s) 287, 726
MboI GATC 2 cut(s) 285, 724
MboII GAAGA 5 cut(s) 63, 719, 878, 911, 929
MfeI CAATTG 1 cut(s) 849
MhlI GDGCHC 2 cut(s) 219, 808
MluCI AATT 9 cut(s) 34, 475, 690, 764, 827, 842, 849, 921, 1014
MmeI TCCRAC 1 cut(s) 699
Mph1103I ATGCAT 1 cut(s) 878
MroXI GAANNNNTTC 1 cut(s) 795
MseI TTAA 4 cut(s) 338, 689, 974, 1022
MspI CCGG 5 cut(s) 200, 208, 275, 560, 673
MspR9I CCNGG 3 cut(s) 154, 200, 370
MunI CAATTG 1 cut(s) 849
MvaI CCWGG 2 cut(s) 154, 370
MwoI GCNNNNNNNGC 3 cut(s) 332, 461, 662
NciI CCSGG 1 cut(s) 200
NdeI CATATG 1 cut(s) 752
NdeII GATC 2 cut(s) 285, 724
NlaIII CATG 3 cut(s) 235, 638, 780
NlaIV GGNNCC 1 cut(s) 906
NmuCI GTSAC 2 cut(s) 135, 853
NsiI ATGCAT 1 cut(s) 878
NspV TTCGAA 1 cut(s) 942
PasI CCCWGGG 1 cut(s) 369
PdmI GAANNNNTTC 1 cut(s) 795
PfeI GAWTC 1 cut(s) 176
Pfl23II CGTACG 1 cut(s) 521
PfoI TCCNGGA 1 cut(s) 198
PkrI GCNGC 1 cut(s) 230
Ppu21I YACGTR 1 cut(s) 525
PshBI ATTAAT 1 cut(s) 689
Psp6I CCWGG 2 cut(s) 152, 368
PspGI CCWGG 2 cut(s) 152, 368
PspLI CGTACG 1 cut(s) 521
PspN4I GGNNCC 1 cut(s) 906
PspPI GGNCC 2 cut(s) 210, 291
RsaI GTAC 1 cut(s) 523
RsaNI GTAC 1 cut(s) 522
Rsr2I CGGWCCG 1 cut(s) 210
RsrII CGGWCCG 1 cut(s) 210
SaqAI TTAA 4 cut(s) 338, 689, 974, 1022
SatI GCNGC 1 cut(s) 229
Sau3AI GATC 2 cut(s) 285, 724
Sau96I GGNCC 2 cut(s) 210, 291
ScrFI CCNGG 3 cut(s) 154, 200, 370
SduI GDGCHC 2 cut(s) 219, 808
SexAI ACCWGGT 1 cut(s) 152
SfaNI GCATC 1 cut(s) 863
SfuI TTCGAA 1 cut(s) 942
SinI GGWCC 2 cut(s) 210, 291
SmlI CTYRAG 1 cut(s) 884
SmoI CTYRAG 1 cut(s) 884
Sse9I AATT 9 cut(s) 34, 475, 690, 764, 827, 842, 849, 921, 1014
SsiI CCGC 1 cut(s) 389
SspMI CTAG 1 cut(s) 896
StyD4I CCNGG 3 cut(s) 152, 198, 368
StyI CCWWGG 2 cut(s) 381, 858
TaaI ACNGT 2 cut(s) 102, 214
TaiI ACGT 4 cut(s) 145, 527, 631, 930
TaqI TCGA 1 cut(s) 942
TasI AATT 9 cut(s) 34, 475, 690, 764, 827, 842, 849, 921, 1014
TfiI GAWTC 1 cut(s) 176
Tru1I TTAA 4 cut(s) 338, 689, 974, 1022
Tru9I TTAA 4 cut(s) 338, 689, 974, 1022
TscAI CASTG 1 cut(s) 600
TseFI GTSAC 2 cut(s) 135, 853
TseI GCWGC 1 cut(s) 228
Tsp45I GTSAC 2 cut(s) 135, 853
TspDTI ATGAA 4 cut(s) 521, 522, 651, 984
TspGWI ACGGA 1 cut(s) 929
TspRI CASTG 1 cut(s) 600
VpaK11BI GGWCC 2 cut(s) 210, 291
VspI ATTAAT 1 cut(s) 689
XapI RAATTY 2 cut(s) 764, 842
XbaI TCTAGA 1 cut(s) 895
XmiI GTMKAC 3 cut(s) 412, 631, 1029
XmnI GAANNNNTTC 1 cut(s) 795
XspI CTAG 1 cut(s) 896
ZraI GACGTC 1 cut(s) 629
Zsp2I ATGCAT 1 cut(s) 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.