AT5G45290

RING-H2 zinc finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005028
Physical Location & Seq
Reverse (-)
18349697 .. 18352637
2941 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G45290.1

Sequence Viewer

Length: 1638 bp
ATGGGATCTGGCCGGAGCAAATCATGTCTAGGCTCGTCGGCGATGGCGAGAGAAGCTTCTGACGTGGCTCAACCTCCTCCGTCGTCTTCTAGTGGTGGTGGAAGGAGAAGAAGATCAAAGAGTCTTTTTTGTGGTCTTCACTCTTCTTGTCTTGCTTCATCTTCTACTTCTCATTACAGTGATGATGATAATGATGAACAGGTGTGTAATGGATGGATGAGGAAGAGTCATGGAAGTGTTACACGGCGTAGAATGGAATCGAGAAGAGATGCATCAGATTGCTATGATAAAGAATTGAAAGATGATAAGAAATCTGAAAACGAAAGACATTGTGATGAGCTTGAGCTTGATGATGAGTGTGGTGTTGAAGAAGAAGAAGAGGAAGCTAGTGTAAGCAATGTACAGATGAGAAATGTTAGCGGTTCGATTCAAGAATCTTCTACTCCAGGTCGAGTTTTTTCGCATTTCAAATTCATTCCTGGTAACATAAGCTCTAGACTTAGCAGAGCTTCAAGTTCAAGATCCTTCAACACGACTTATCCAGTCTCTTCTTCTCATAGAGAAGATGGTGTTACTTCACCTTCAGAATCTGCTATTAGGCTCAATGATAGAGTTGAGTCTCCGAGAATTCCTGTGATTGACAATGTAGTTAGAGATATTGATGCCATGAGATTTGGGGAAGATGGTAGCTTACGTTCTCCGAGAGTAGTTAATGGTATGGATAGTACTTATGCTGGACTTCTTGATAGAAGACATGTTGTACGAGAACCTTCTATTGAGCGCAATGTTAGATTTAGTAGAACTCTGAGTGTTGGAAGACTCCGTGATAGAGTTCTTCGCCGATCTTCACTCTCTGATTTCGCATTCCGTCCCTCGCCACATCAGGGAGAAGAAGATACTGATCTCTTCTCTGCTGAAACCGCAGCAGCAGAGGATGCTCCTGTGACTTCAACGTCTATACTAAATCGTTCCGCTTCTAGCATTCGAAGGACCTTATTCGGCATTCAGGATCATGAGACACCAGCTGCTCTTGTGAGAGAAGGGAGGTATCAAGGTTTATTAGAACACAGATCAGATTTCCTTGAAAGAAGGAGGAGAATAAGATCTCAGGTTCGCGCTCTTCAAAGATTAGGAAGCCGGTTTGAGAATGTCGCAGTCCATCATGATAGATCTTGTGTCTTATCAGGTCAAGATCAAGCAGGTCGCTGCACATGCCGTGCAGTTACTAACCGTGGTTCGACAACAGCAACAACCGATGAAACAAATGCAAGAGCTAGCATCTCAAGGATAGTATTGCTAGCTGAAGCTCTCTTCGAGGTTCTTGATGAAATTCATCAGCAATCTGTTGTCTTATCCTCTCAACAACCATCAGTATCATCTATAGGATCTGTTCCTGCACCTAACGACGTTGTGGATTTATTACCGATAAAACTTTATACTAAGTCACAAAGCGAGGATCCATCACAATGTTACATATGCCTTGTGGAATATGAAGAAGCAGACTCTATAAGGACACTTCCTTGTCATCATGAATTCCACAAAACCTGTGTAGATAAATGGCTCAAGGAGATTCACAGAGTATGTCCGCTGTGTCGAGGAGATATTTGTAGACATGATCCATCATCGGAACAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

545

Amino Acids

60.77

Weight (kDa)

6.37

Isoelectric Point (pI)

71.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 489 - 532 1.3e-12 Ring finger domain
zf-rbx1 PF12678 489 - 532 9.1e-08 RING-H2 zinc finger domain
zf-RING_11 PF17123 489 - 517 9e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 489 - 531 1.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 490 - 531 2.2e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 952
Acc36I ACCTGC 1 cut(s) 1190
AccI GTMKAC 1 cut(s) 1609
AccII CGCG 1 cut(s) 1116
AciI CCGC 4 cut(s) 420, 921, 972, 1586
AclWI GGATC 7 cut(s) 13, 516, 1017, 1393, 1451, 1464, 1610
AcoI YGGCCR 1 cut(s) 10
AcsI RAATTY 4 cut(s) 470, 627, 1329, 1532
AcuI CTGAAG 2 cut(s) 567, 1323
AfaI GTAC 3 cut(s) 402, 727, 762
AfiI CCNNNNNNNGG 1 cut(s) 884
AflIII ACRYGT 1 cut(s) 754
AjiI CACGTC 1 cut(s) 64
AjnI CCWGG 2 cut(s) 445, 478
Alw26I GTCTC 3 cut(s) 550, 624, 1010
AlwI GGATC 7 cut(s) 13, 516, 1017, 1393, 1451, 1464, 1610
AlwNI CAGNNNCTG 1 cut(s) 590
AoxI GGCC 1 cut(s) 10
ApeKI GCWGC 4 cut(s) 923, 926, 1025, 1206
ApoI RAATTY 4 cut(s) 470, 627, 1329, 1532
AspLEI GCGC 2 cut(s) 783, 1118
AspS9I GGNCC 1 cut(s) 990
AsuHPI GGTGA 1 cut(s) 570
AsuII TTCGAA 1 cut(s) 985
AsuNHI GCTAGC 2 cut(s) 1274, 1297
AvaII GGWCC 1 cut(s) 990
BamHI GGATCC 1 cut(s) 1456
BbsI GAAGAC 4 cut(s) 78, 128, 757, 823
BbvI GCAGC 4 cut(s) 935, 938, 1012, 1193
BccI CCATC 8 cut(s) 37, 207, 560, 677, 1167, 1375, 1468, 1627
BceAI ACGGC 2 cut(s) 260, 1200
BciT130I CCWGG 2 cut(s) 447, 480
BcoDI GTCTC 3 cut(s) 550, 624, 1010
BfaI CTAG 7 cut(s) 29, 90, 387, 495, 978, 1275, 1298
BfmI CTRYAG 1 cut(s) 1380
BfuAI ACCTGC 1 cut(s) 1190
BglII AGATCT 2 cut(s) 1103, 1169
BisI GCNGC 4 cut(s) 924, 927, 1026, 1207
BlsI GCNGC 4 cut(s) 925, 928, 1027, 1208
BmcAI AGTACT 1 cut(s) 727
Bme1390I CCNGG 2 cut(s) 447, 480
Bme18I GGWCC 1 cut(s) 990
BmgBI CACGTC 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 990
BmiI GGNNCC 1 cut(s) 1458
BmrFI CCNGG 2 cut(s) 447, 480
BmsI GCATC 5 cut(s) 259, 281, 652, 925, 1287
BmtI GCTAGC 2 cut(s) 1278, 1301
BpiI GAAGAC 4 cut(s) 78, 128, 757, 823
BpmI CTGGAG 1 cut(s) 429
Bpu14I TTCGAA 1 cut(s) 985
BpuEI CTTGAG 3 cut(s) 362, 1267, 1547
BsaBI GATNNNNATC 1 cut(s) 900
BsaJI CCNNGG 1 cut(s) 1231
Bsc4I CCNNNNNNNGG 1 cut(s) 884
Bse118I RCCGGY 1 cut(s) 1137
Bse1I ACTGG 1 cut(s) 542
Bse3DI GCAATG 2 cut(s) 403, 790
Bse8I GATNNNNATC 1 cut(s) 900
BseBI CCWGG 2 cut(s) 447, 480
BseDI CCNNGG 1 cut(s) 1231
BseGI GGATG 3 cut(s) 218, 222, 940
BseJI GATNNNNATC 1 cut(s) 900
BseLI CCNNNNNNNGG 1 cut(s) 884
BseMI GCAATG 2 cut(s) 403, 790
BseMII CTCAG 2 cut(s) 797, 1121
BseNI ACTGG 1 cut(s) 542
BseRI GAGGAG 3 cut(s) 66, 1108, 1611
BseXI GCAGC 4 cut(s) 935, 938, 1012, 1193
BsgI GTGCAG 3 cut(s) 1192, 1239, 1380
Bsh1236I CGCG 1 cut(s) 1116
BshFI GGCC 1 cut(s) 12
BsiSI CCGG 2 cut(s) 13, 1138
BslFI GGGAC 1 cut(s) 855
BslI CCNNNNNNNGG 1 cut(s) 884
BsmAI GTCTC 3 cut(s) 550, 624, 1010
BsmFI GGGAC 1 cut(s) 855
BsmI GAATGC 3 cut(s) 863, 981, 1002
BsnI GGCC 1 cut(s) 12
Bsp119I TTCGAA 1 cut(s) 985
Bsp1407I TGTACA 1 cut(s) 400
BspACI CCGC 4 cut(s) 420, 921, 972, 1586
BspANI GGCC 1 cut(s) 12
BspCNI CTCAG 2 cut(s) 798, 1120
BspFNI CGCG 1 cut(s) 1116
BspHI TCATGA 3 cut(s) 1012, 1162, 1528
BspLI GGNNCC 1 cut(s) 1458
BspMI ACCTGC 1 cut(s) 1190
BspOI GCTAGC 2 cut(s) 1278, 1301
BspPI GGATC 7 cut(s) 13, 516, 1017, 1393, 1451, 1464, 1610
BspQI GCTCTTC 1 cut(s) 1125
BspT104I TTCGAA 1 cut(s) 985
BsrDI GCAATG 2 cut(s) 403, 790
BsrFI RCCGGY 1 cut(s) 1137
BsrGI TGTACA 1 cut(s) 400
BsrI ACTGG 1 cut(s) 542
BssAI RCCGGY 1 cut(s) 1137
BssECI CCNNGG 1 cut(s) 1231
Bst2UI CCWGG 2 cut(s) 447, 480
Bst4CI ACNGT 2 cut(s) 179, 1232
Bst6I CTCTTC 8 cut(s) 148, 218, 259, 372, 553, 911, 1125, 1316
BstAPI GCANNNNNTGC 1 cut(s) 935
BstAUI TGTACA 1 cut(s) 400
BstBI TTCGAA 1 cut(s) 985
BstC8I GCNNGC 2 cut(s) 1276, 1299
BstDEI CTNAG 4 cut(s) 500, 806, 1107, 1440
BstDSI CCRYGG 1 cut(s) 1231
BstF5I GGATG 3 cut(s) 218, 222, 940
BstFNI CGCG 1 cut(s) 1116
BstHHI GCGC 2 cut(s) 783, 1118
BstMAI GTCTC 3 cut(s) 550, 624, 1010
BstMWI GCNNNNNNNGC 4 cut(s) 53, 920, 935, 1212
BstNI CCWGG 2 cut(s) 447, 480
BstNSI RCATGY 2 cut(s) 758, 1215
BstSCI CCNGG 2 cut(s) 445, 478
BstSFI CTRYAG 1 cut(s) 1380
BstUI CGCG 1 cut(s) 1116
BstV1I GCAGC 4 cut(s) 935, 938, 1012, 1193
BstV2I GAAGAC 4 cut(s) 78, 128, 757, 823
BstX2I RGATCY 6 cut(s) 5, 521, 1103, 1169, 1385, 1456
BstYI RGATCY 6 cut(s) 5, 521, 1103, 1169, 1385, 1456
BsuRI GGCC 1 cut(s) 12
BtgI CCRYGG 1 cut(s) 1231
BtgZI GCGATG 1 cut(s) 56
BtrI CACGTC 1 cut(s) 64
BtsCI GGATG 3 cut(s) 218, 222, 940
BtsIMutI CAGTG 1 cut(s) 184
BveI ACCTGC 1 cut(s) 1190
Cac8I GCNNGC 2 cut(s) 1276, 1299
CaiI CAGNNNCTG 1 cut(s) 590
CciI TCATGA 3 cut(s) 1012, 1162, 1528
CfoI GCGC 2 cut(s) 783, 1118
Cfr10I RCCGGY 1 cut(s) 1137
Cfr13I GGNCC 1 cut(s) 990
Csp6I GTAC 3 cut(s) 401, 726, 761
CviAII CATG 9 cut(s) 24, 230, 667, 755, 1013, 1163, 1212, 1529, 1613
CviQI GTAC 3 cut(s) 401, 726, 761
DdeI CTNAG 4 cut(s) 500, 806, 1107, 1440
DrdI GACNNNNNNGTC 1 cut(s) 952
DseDI GACNNNNNNGTC 1 cut(s) 952
EaeI YGGCCR 1 cut(s) 10
Eam1104I CTCTTC 8 cut(s) 148, 218, 259, 372, 553, 911, 1125, 1316
EarI CTCTTC 8 cut(s) 148, 218, 259, 372, 553, 911, 1125, 1316
Eco47I GGWCC 1 cut(s) 990
Eco57I CTGAAG 2 cut(s) 567, 1323
EcoO109I RGGNCCY 1 cut(s) 990
EcoRI GAATTC 2 cut(s) 627, 1532
EcoRII CCWGG 2 cut(s) 445, 478
EcoT22I ATGCAT 1 cut(s) 274
FaeI CATG 9 cut(s) 27, 233, 670, 758, 1016, 1166, 1215, 1532, 1616
FaqI GGGAC 1 cut(s) 855
FatI CATG 9 cut(s) 23, 229, 666, 754, 1012, 1162, 1211, 1528, 1612
FauNDI CATATG 1 cut(s) 1475
FblI GTMKAC 1 cut(s) 1609
Fnu4HI GCNGC 4 cut(s) 924, 927, 1026, 1207
FokI GGATG 3 cut(s) 225, 229, 947
Fsp4HI GCNGC 4 cut(s) 924, 927, 1026, 1207
FspBI CTAG 7 cut(s) 29, 90, 387, 495, 978, 1275, 1298
GlaI GCGC 2 cut(s) 782, 1117
GluI GCNGC 4 cut(s) 924, 927, 1026, 1207
GsuI CTGGAG 1 cut(s) 429
HaeIII GGCC 1 cut(s) 12
HapII CCGG 2 cut(s) 13, 1138
HhaI GCGC 2 cut(s) 783, 1118
Hin1II CATG 9 cut(s) 27, 233, 670, 758, 1016, 1166, 1215, 1532, 1616
Hin6I GCGC 2 cut(s) 781, 1116
HinP1I GCGC 2 cut(s) 781, 1116
HindIII AAGCTT 1 cut(s) 54
HpaII CCGG 2 cut(s) 13, 1138
HphI GGTGA 1 cut(s) 570
Hpy166II GTNNAC 1 cut(s) 1610
Hpy8I GTNNAC 1 cut(s) 1610
Hpy99I CGWCG 3 cut(s) 40, 85, 1409
HpyAV CCTTC 7 cut(s) 96, 535, 591, 780, 981, 1034, 1083
HpyCH4III ACNGT 2 cut(s) 179, 1232
HpyCH4IV ACGT 4 cut(s) 63, 694, 953, 1407
HpyCH4V TGCA 5 cut(s) 272, 1209, 1220, 1268, 1397
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 920, 935, 1212
HpyF3I CTNAG 4 cut(s) 500, 806, 1107, 1440
HpySE526I ACGT 4 cut(s) 63, 694, 953, 1407
Hsp92II CATG 9 cut(s) 27, 233, 670, 758, 1016, 1166, 1215, 1532, 1616
HspAI GCGC 2 cut(s) 781, 1116
LguI GCTCTTC 1 cut(s) 1125
LmnI GCTCC 2 cut(s) 15, 943
Lsp1109I GCAGC 4 cut(s) 935, 938, 1012, 1193
LweI GCATC 5 cut(s) 259, 281, 652, 925, 1287
MaeI CTAG 7 cut(s) 29, 90, 387, 495, 978, 1275, 1298
MaeII ACGT 4 cut(s) 63, 694, 953, 1407
MaeIII GTNAC 7 cut(s) 238, 482, 571, 943, 1222, 1443, 1469
MflI RGATCY 6 cut(s) 5, 521, 1103, 1169, 1385, 1456
MluCI AATT 5 cut(s) 293, 470, 627, 1329, 1532
MlyI GAGTC 5 cut(s) 130, 235, 626, 813, 1496
MmeI TCCRAC 1 cut(s) 793
Mph1103I ATGCAT 1 cut(s) 274
MseI TTAA 1 cut(s) 711
MslI CAYNNNNRTG 4 cut(s) 177, 234, 333, 1465
MspA1I CMGCKG 2 cut(s) 1025, 1588
MspI CCGG 2 cut(s) 13, 1138
MspR9I CCNGG 2 cut(s) 447, 480
Mva1269I GAATGC 3 cut(s) 863, 981, 1002
MvaI CCWGG 2 cut(s) 447, 480
MvnI CGCG 1 cut(s) 1116
MwoI GCNNNNNNNGC 4 cut(s) 53, 920, 935, 1212
NdeI CATATG 1 cut(s) 1475
NheI GCTAGC 2 cut(s) 1274, 1297
NlaIII CATG 9 cut(s) 27, 233, 670, 758, 1016, 1166, 1215, 1532, 1616
NlaIV GGNNCC 1 cut(s) 1458
NmuCI GTSAC 2 cut(s) 943, 1443
NsiI ATGCAT 1 cut(s) 274
NspI RCATGY 2 cut(s) 758, 1215
NspV TTCGAA 1 cut(s) 985
PagI TCATGA 3 cut(s) 1012, 1162, 1528
PciI ACATGT 1 cut(s) 754
PciSI GCTCTTC 1 cut(s) 1125
PcsI WCGNNNNNNNCGW 1 cut(s) 44
PctI GAATGC 3 cut(s) 863, 981, 1002
PfeI GAWTC 5 cut(s) 257, 427, 434, 587, 1570
PkrI GCNGC 4 cut(s) 925, 928, 1027, 1208
PleI GAGTC 5 cut(s) 129, 234, 625, 813, 1496
PpsI GAGTC 5 cut(s) 129, 234, 625, 813, 1496
PpuMI RGGWCCY 1 cut(s) 990
PscI ACATGT 1 cut(s) 754
Psp5II RGGWCCY 1 cut(s) 990
Psp6I CCWGG 2 cut(s) 445, 478
PspGI CCWGG 2 cut(s) 445, 478
PspN4I GGNNCC 1 cut(s) 1458
PspPI GGNCC 1 cut(s) 990
PspPPI RGGWCCY 1 cut(s) 990
PsrI GAACNNNNNNTAC 2 cut(s) 679, 711
PstNI CAGNNNCTG 1 cut(s) 590
PsuI RGATCY 6 cut(s) 5, 521, 1103, 1169, 1385, 1456
PvuII CAGCTG 1 cut(s) 1025
RsaI GTAC 3 cut(s) 402, 727, 762
RsaNI GTAC 3 cut(s) 401, 726, 761
RseI CAYNNNNRTG 4 cut(s) 177, 234, 333, 1465
SapI GCTCTTC 1 cut(s) 1125
SaqAI TTAA 1 cut(s) 711
SatI GCNGC 4 cut(s) 924, 927, 1026, 1207
Sau96I GGNCC 1 cut(s) 990
ScaI AGTACT 1 cut(s) 727
SchI GAGTC 5 cut(s) 130, 235, 626, 813, 1496
ScrFI CCNGG 2 cut(s) 447, 480
SfaNI GCATC 5 cut(s) 259, 281, 652, 925, 1287
SfcI CTRYAG 1 cut(s) 1380
SfuI TTCGAA 1 cut(s) 985
SinI GGWCC 1 cut(s) 990
SmiMI CAYNNNNRTG 4 cut(s) 177, 234, 333, 1465
SmlI CTYRAG 3 cut(s) 341, 1282, 1562
SmoI CTYRAG 3 cut(s) 341, 1282, 1562
Sse9I AATT 5 cut(s) 293, 470, 627, 1329, 1532
SsiI CCGC 4 cut(s) 420, 921, 972, 1586
SspMI CTAG 7 cut(s) 29, 90, 387, 495, 978, 1275, 1298
StyD4I CCNGG 2 cut(s) 445, 478
TaaI ACNGT 2 cut(s) 179, 1232
TaiI ACGT 4 cut(s) 66, 697, 956, 1410
TaqI TCGA 7 cut(s) 260, 425, 451, 985, 1238, 1314, 1594
TasI AATT 5 cut(s) 293, 470, 627, 1329, 1532
TatI WGTACW 2 cut(s) 400, 725
TfiI GAWTC 5 cut(s) 257, 427, 434, 587, 1570
Tru1I TTAA 1 cut(s) 711
Tru9I TTAA 1 cut(s) 711
TscAI CASTG 1 cut(s) 184
TseFI GTSAC 2 cut(s) 943, 1443
TseI GCWGC 4 cut(s) 923, 926, 1025, 1206
Tsp45I GTSAC 2 cut(s) 943, 1443
TspDTI ATGAA 8 cut(s) 147, 210, 463, 1272, 1322, 1341, 1506, 1545
TspGWI ACGGA 3 cut(s) 69, 812, 857
TspRI CASTG 1 cut(s) 184
VpaK11BI GGWCC 1 cut(s) 990
XapI RAATTY 4 cut(s) 470, 627, 1329, 1532
XbaI TCTAGA 1 cut(s) 494
XceI RCATGY 2 cut(s) 758, 1215
XmiI GTMKAC 1 cut(s) 1609
XspI CTAG 7 cut(s) 29, 90, 387, 495, 978, 1275, 1298
ZrmI AGTACT 1 cut(s) 727
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.