MD16G1287000.v1.1

RING-like zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005028
Physical Location & Seq
Forward (+)
41122895 .. 41130836
7942 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1287000.v1.1.491

Sequence Viewer

Length: 1755 bp
ATGGGATCGGGCGGCAGCAAAGCGACGACGTCGTCGGCATTGCCTTCTTCGACGTCGTCGTTTGGTGGAAATGGCCGGAACCGGAGGCGTCGATCCAAATCCAAATGCCGCCGACTATTCAAGTCGTCATCTTGTCTGCGACTTAGAACCAAATCTCGATCTCACGACTGCGACAATGAACACCAGGTTTGTGATCATCAGAACAAAGGAATCAGGAATAATGCTCCATGTGCAAGCCTGAATGACACAGAGCCAGATCATGCAAACATTGAATGTTTCAGAAAGGTCAAAGTTGAACAACCTGATGAAATGCCTTGTATAACTCCAAATGTTGAGCTGAATGAATGGGCTCAGGCTAGTATCACTGAAACTGCTTCCATAACTGCTAGCAGCTCTGCCCAACCTTCTGCTACTCCAACTTTGAACCCTTCAAGTCGTTTCCTTTCTCGCTTTAGCTTCATTCCAGGTAATGTAAGCTTCAGGTTGAGCCGGGCAACAAGTGTAGGGTCATCCAGGTCTTATCCTCTATCTTCGACAAGTCTCACAATACTGAACAATGAGGATGAGCTTCATCTACATGCAAGGCCTGCTAGCAGTTTGATAGATACAGATGAAAGCCAACAAGCCAATGGCTTTCTTCCAGAATCACATGTTAATAGGATCCCAACACATATTTATAACCACACTTCTGGCAATGTACAATTAAATACTCCGGGATCTGTTTTTTCTGACCACTTGCAAGATAACCAAACAAATTCTTCTAGACGTGCTGTTGGTAGAAATACTAACGGTGCAATAATGGGGGTTGGTGTAAACTCGTGTTCTACTAGAATTCATAATGAAATAGGGAGCAACAGAACAAGACCTTCTGATAGGCGTTCAGGAGCTCGGGAACCTGTTGAACGGAATGTTCGTTTCAGCAGAACCTTAAGTGTTGGAAGACTTCGTGACAGGGTACTACGTCGATCATCATTATCTGATTTAACGTTTTCTTCTTTGCAACAAGAAGTGAGAGATACAAATCAGAGAGATGGTACACAGAGTTTGGAACCTGAAGGTAATGTTGTAAACTCCGAAAATGGGTCTGGTTATTCTCCATCTAATATGTCTAGTTCATTGTTTGGCATTCAAGACTATGGAGTGGAAACATCAAGACCAAGAGAAGCTAGGTATCATGATCTACTTGAACACAGATCAAATTTCCTAGAGCGGAGGAGAAGAATACGATCTCAGGTTCGTGCACTTCAGCGGTTGGGGAGCCGCTTCGAAAACATATCTGGACATGAGAGGTCTTGTATCTTATCTGGTCAACAGAGAACAGGTCGTTGTACATGTCGTATGAGTAATCGAGATGCCCGTCCAAATGATGACAACAGTGCTAGGGCTAGCATATCAAGAATAGTTATGTTAGCTGAGGCTTTGTTTGAGGTTCTGGATGAAATTCACCAGCAATCCGTGGTTTTATCTTCCCGACCTTCTGTATCTTCAATTGGATCTGTTCCTGCTCCGAATGAAGTTGTGGAATCTTTGCCTGTCAAATTGTTTTCGAAGTTGCAAAAAAATCAGTATGAGGAGGCTGCACAATGCTACATATGCCTTGTGGAGTATGAAGAAGGAGACAGAATGAGAATACTGCCTTGCCATCATGAGTTTCATAAAACTTGTATTGACAAGTGGCTCAAGGAGATTCACAGGGTGTGCCCGCTCTGTCGCAGGGATATCTGTAGATCTGATGCATCGCCTGCAGAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

585

Amino Acids

65.18

Weight (kDa)

9.19

Isoelectric Point (pI)

65.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 527 - 571 2e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 528 - 571 3.8e-13 Ring finger domain
zf-RING_11 PF17123 528 - 556 7.1e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 528 - 570 4.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 529 - 570 1.5e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 678
AasI GACNNNNNNGTC 1 cut(s) 31
AatII GACGTC 2 cut(s) 32, 56
AccBSI CCGCTC 2 cut(s) 1210, 1705
AciI CCGC 6 cut(s) 12, 109, 1210, 1249, 1261, 1703
AclI AACGTT 1 cut(s) 986
AclWI GGATC 6 cut(s) 13, 87, 655, 668, 724, 1501
AcoI YGGCCR 1 cut(s) 73
AcsI RAATTY 4 cut(s) 754, 831, 1198, 1440
AcuI CTGAAG 3 cut(s) 463, 1074, 1229
AcyI GRCGYC 3 cut(s) 29, 53, 88
AdeI CACNNNGTG 1 cut(s) 1696
AfaI GTAC 4 cut(s) 699, 957, 1036, 1330
AfiI CCNNNNNNNGG 1 cut(s) 1080
AflII CTTAAG 1 cut(s) 928
AflIII ACRYGT 2 cut(s) 649, 1331
AgsI TTSAA 9 cut(s) 121, 272, 296, 424, 432, 902, 1130, 1187, 1488
AjiI CACGTC 1 cut(s) 767
AjnI CCWGG 3 cut(s) 183, 463, 512
AluBI AGCT 8 cut(s) 337, 393, 456, 477, 568, 887, 1166, 1412
AluI AGCT 8 cut(s) 337, 393, 456, 477, 568, 887, 1166, 1412
Alw21I GWGCWC 2 cut(s) 889, 1243
Alw26I GTCTC 2 cut(s) 545, 1611
Alw44I GTGCAC 1 cut(s) 1239
AlwI GGATC 6 cut(s) 13, 87, 655, 668, 724, 1501
Ama87I CYCGRG 1 cut(s) 888
AoxI GGCC 2 cut(s) 73, 584
ApaLI GTGCAC 1 cut(s) 1239
ApeKI GCWGC 3 cut(s) 15, 390, 1577
ApoI RAATTY 4 cut(s) 754, 831, 1198, 1440
AsuC2I CCSGG 2 cut(s) 491, 714
AsuHPI GGTGA 1 cut(s) 1436
AsuII TTCGAA 2 cut(s) 1266, 1547
AsuNHI GCTAGC 3 cut(s) 386, 590, 1385
AvaI CYCGRG 1 cut(s) 888
BaeGI GKGCMC 2 cut(s) 1243, 1703
BamHI GGATCC 1 cut(s) 660
BanII GRGCYC 2 cut(s) 352, 889
BauI CACGAG 1 cut(s) 817
BbsI GAAGAC 1 cut(s) 946
Bbv12I GWGCWC 2 cut(s) 889, 1243
BbvCI CCTCAGC 1 cut(s) 1413
BbvI GCAGC 3 cut(s) 27, 402, 1564
BccI CCATC 3 cut(s) 1025, 1105, 1650
BciT130I CCWGG 3 cut(s) 185, 465, 514
BclI TGATCA 1 cut(s) 193
BcnI CCSGG 2 cut(s) 491, 714
BcoDI GTCTC 2 cut(s) 545, 1611
BfmI CTRYAG 2 cut(s) 1723, 1743
BfrI CTTAAG 1 cut(s) 928
BglII AGATCT 1 cut(s) 1727
BisI GCNGC 6 cut(s) 13, 16, 109, 391, 1261, 1578
BlsI GCNGC 6 cut(s) 14, 17, 110, 392, 1262, 1579
Bme1390I CCNGG 5 cut(s) 185, 465, 491, 514, 714
BmeT110I CYCGRG 1 cut(s) 888
BmgBI CACGTC 1 cut(s) 767
BmiI GGNNCC 5 cut(s) 80, 662, 894, 1050, 1259
BmrFI CCNGG 5 cut(s) 185, 465, 491, 514, 714
BmsI GCATC 3 cut(s) 1342, 1723, 1745
BmtI GCTAGC 3 cut(s) 390, 594, 1389
BpiI GAAGAC 1 cut(s) 946
Bpu10I CCTNAGC 2 cut(s) 351, 1413
Bpu14I TTCGAA 2 cut(s) 1266, 1547
BpuEI CTTGAG 1 cut(s) 1664
BpuMI CCSGG 2 cut(s) 491, 714
BsaBI GATNNNNATC 2 cut(s) 97, 1020
BsaHI GRCGYC 3 cut(s) 29, 53, 88
BsaJI CCNNGG 1 cut(s) 1455
BsaWI WCCGGW 1 cut(s) 81
Bsc4I CCNNNNNNNGG 1 cut(s) 1080
Bse3DI GCAATG 2 cut(s) 38, 700
Bse8I GATNNNNATC 2 cut(s) 97, 1020
BseBI CCWGG 3 cut(s) 185, 465, 514
BseDI CCNNGG 1 cut(s) 1455
BseGI GGATG 3 cut(s) 509, 568, 1441
BseJI GATNNNNATC 2 cut(s) 97, 1020
BseLI CCNNNNNNNGG 1 cut(s) 1080
BseMI GCAATG 2 cut(s) 38, 700
BseMII CTCAG 3 cut(s) 365, 1244, 1404
BseRI GAGGAG 2 cut(s) 1228, 1586
BseSI GKGCMC 2 cut(s) 1243, 1703
BseXI GCAGC 3 cut(s) 27, 402, 1564
BsgI GTGCAG 1 cut(s) 1563
BshFI GGCC 2 cut(s) 75, 586
BsiHKAI GWGCWC 2 cut(s) 889, 1243
BsiHKCI CYCGRG 1 cut(s) 888
BsiSI CCGG 4 cut(s) 76, 82, 490, 713
BslI CCNNNNNNNGG 1 cut(s) 1080
BsmAI GTCTC 2 cut(s) 545, 1611
BsmI GAATGC 1 cut(s) 1125
BsnI GGCC 2 cut(s) 75, 586
BsoBI CYCGRG 1 cut(s) 888
Bsp119I TTCGAA 2 cut(s) 1266, 1547
Bsp1286I GDGCHC 4 cut(s) 352, 889, 1243, 1703
Bsp1407I TGTACA 2 cut(s) 697, 1328
BspACI CCGC 6 cut(s) 12, 109, 1210, 1249, 1261, 1703
BspANI GGCC 2 cut(s) 75, 586
BspCNI CTCAG 3 cut(s) 364, 1243, 1405
BspHI TCATGA 2 cut(s) 1174, 1645
BspLI GGNNCC 5 cut(s) 80, 662, 894, 1050, 1259
BspMAI CTGCAG 1 cut(s) 1747
BspOI GCTAGC 3 cut(s) 390, 594, 1389
BspPI GGATC 6 cut(s) 13, 87, 655, 668, 724, 1501
BspT104I TTCGAA 2 cut(s) 1266, 1547
BspTI CTTAAG 1 cut(s) 928
BsrBI CCGCTC 2 cut(s) 1210, 1705
BsrDI GCAATG 2 cut(s) 38, 700
BsrGI TGTACA 2 cut(s) 697, 1328
BssECI CCNNGG 1 cut(s) 1455
BssNI GRCGYC 3 cut(s) 29, 53, 88
BssSI CACGAG 1 cut(s) 817
Bst2BI CACGAG 1 cut(s) 817
Bst2UI CCWGG 3 cut(s) 185, 465, 514
Bst4CI ACNGT 2 cut(s) 791, 1376
BstACI GRCGYC 3 cut(s) 29, 53, 88
BstAFI CTTAAG 1 cut(s) 928
BstAPI GCANNNNNTGC 2 cut(s) 587, 1742
BstAUI TGTACA 2 cut(s) 697, 1328
BstBI TTCGAA 2 cut(s) 1266, 1547
BstC8I GCNNGC 7 cut(s) 235, 388, 588, 592, 1387, 1703, 1743
BstDEI CTNAG 4 cut(s) 143, 351, 1230, 1413
BstDSI CCRYGG 1 cut(s) 1455
BstF5I GGATG 3 cut(s) 509, 568, 1441
BstMAI GTCTC 2 cut(s) 545, 1611
BstMWI GCNNNNNNNGC 4 cut(s) 230, 587, 1593, 1742
BstNI CCWGG 3 cut(s) 185, 465, 514
BstNSI RCATGY 3 cut(s) 581, 653, 1335
BstSCI CCNGG 5 cut(s) 183, 463, 489, 512, 712
BstSFI CTRYAG 2 cut(s) 1723, 1743
BstSLI GKGCMC 2 cut(s) 1243, 1703
BstV1I GCAGC 3 cut(s) 27, 402, 1564
BstV2I GAAGAC 1 cut(s) 946
BstX2I RGATCY 4 cut(s) 660, 716, 1493, 1727
BstXI CCANNNNNNTGG 1 cut(s) 689
BstYI RGATCY 4 cut(s) 660, 716, 1493, 1727
BsuRI GGCC 2 cut(s) 75, 586
BtgI CCRYGG 1 cut(s) 1455
BtgZI GCGATG 1 cut(s) 1722
BtrI CACGTC 1 cut(s) 767
BtsCI GGATG 3 cut(s) 509, 568, 1441
BtsIMutI CAGTG 2 cut(s) 363, 1381
Cac8I GCNNGC 7 cut(s) 235, 388, 588, 592, 1387, 1703, 1743
CciI TCATGA 2 cut(s) 1174, 1645
CseI GACGC 1 cut(s) 77
CsiI ACCWGGT 1 cut(s) 183
Csp6I GTAC 4 cut(s) 698, 956, 1035, 1329
CviAII CATG 8 cut(s) 228, 260, 578, 650, 1175, 1283, 1332, 1646
CviQI GTAC 4 cut(s) 698, 956, 1035, 1329
DdeI CTNAG 4 cut(s) 143, 351, 1230, 1413
DraIII CACNNNGTG 1 cut(s) 1696
DrdI GACNNNNNNGTC 1 cut(s) 31
DseDI GACNNNNNNGTC 1 cut(s) 31
EaeI YGGCCR 1 cut(s) 73
Ecl136II GAGCTC 1 cut(s) 887
Eco147I AGGCCT 1 cut(s) 586
Eco24I GRGCYC 2 cut(s) 352, 889
Eco32I GATATC 1 cut(s) 1720
Eco53kI GAGCTC 1 cut(s) 887
Eco57I CTGAAG 3 cut(s) 463, 1074, 1229
Eco88I CYCGRG 1 cut(s) 888
EcoICRI GAGCTC 1 cut(s) 887
EcoRI GAATTC 1 cut(s) 831
EcoRII CCWGG 3 cut(s) 183, 463, 512
EcoRV GATATC 1 cut(s) 1720
EcoT22I ATGCAT 1 cut(s) 1738
EcoT38I GRGCYC 2 cut(s) 352, 889
FaeI CATG 8 cut(s) 231, 263, 581, 653, 1178, 1286, 1335, 1649
FatI CATG 8 cut(s) 227, 259, 577, 649, 1174, 1282, 1331, 1645
FauI CCCGC 1 cut(s) 1710
FauNDI CATATG 1 cut(s) 1592
FbaI TGATCA 1 cut(s) 193
Fnu4HI GCNGC 6 cut(s) 13, 16, 109, 391, 1261, 1578
FokI GGATG 3 cut(s) 496, 575, 1448
FriOI GRGCYC 2 cut(s) 352, 889
Fsp4HI GCNGC 6 cut(s) 13, 16, 109, 391, 1261, 1578
GluI GCNGC 6 cut(s) 13, 16, 109, 391, 1261, 1578
HaeIII GGCC 2 cut(s) 75, 586
HapII CCGG 4 cut(s) 76, 82, 490, 713
HgaI GACGC 1 cut(s) 77
Hin1I GRCGYC 3 cut(s) 29, 53, 88
Hin1II CATG 8 cut(s) 231, 263, 581, 653, 1178, 1286, 1335, 1649
HincII GTYRAC 1 cut(s) 1310
HindII GTYRAC 1 cut(s) 1310
HindIII AAGCTT 1 cut(s) 475
HinfI GANTC 4 cut(s) 210, 644, 1523, 1687
HpaII CCGG 4 cut(s) 76, 82, 490, 713
HphI GGTGA 1 cut(s) 1436
Hpy166II GTNNAC 5 cut(s) 814, 1037, 1069, 1241, 1310
Hpy188I TCNGA 9 cut(s) 201, 281, 730, 871, 979, 1026, 1075, 1509, 1732
Hpy8I GTNNAC 5 cut(s) 814, 1037, 1069, 1241, 1310
Hpy99I CGWCG 9 cut(s) 28, 31, 34, 37, 55, 58, 61, 93, 966
HpyAV CCTTC 7 cut(s) 54, 414, 438, 876, 1049, 1485, 1607
HpyCH4III ACNGT 2 cut(s) 791, 1376
HpyCH4IV ACGT 5 cut(s) 29, 53, 766, 961, 986
HpyF10VI GCNNNNNNNGC 4 cut(s) 230, 587, 1593, 1742
HpyF3I CTNAG 4 cut(s) 143, 351, 1230, 1413
HpySE526I ACGT 5 cut(s) 29, 53, 766, 961, 986
Hsp92I GRCGYC 3 cut(s) 29, 53, 88
Hsp92II CATG 8 cut(s) 231, 263, 581, 653, 1178, 1286, 1335, 1649
Ksp22I TGATCA 1 cut(s) 193
LmnI GCTCC 5 cut(s) 229, 849, 884, 1257, 1510
Lsp1109I GCAGC 3 cut(s) 27, 402, 1564
LweI GCATC 3 cut(s) 1342, 1723, 1745
MabI ACCWGGT 1 cut(s) 183
MaeII ACGT 5 cut(s) 29, 53, 766, 961, 986
MaeIII GTNAC 1 cut(s) 947
MbiI CCGCTC 2 cut(s) 1210, 1705
MfeI CAATTG 1 cut(s) 1488
MflI RGATCY 4 cut(s) 660, 716, 1493, 1727
MhlI GDGCHC 4 cut(s) 352, 889, 1243, 1703
MluCI AATT 7 cut(s) 701, 754, 831, 1198, 1440, 1488, 1538
MmeI TCCRAC 2 cut(s) 440, 916
MnlI CCTC 9 cut(s) 78, 534, 553, 1206, 1281, 1408, 1420, 1564, 1567
Mph1103I ATGCAT 1 cut(s) 1738
MseI TTAA 4 cut(s) 654, 704, 929, 983
MslI CAYNNNNRTG 1 cut(s) 576
MspA1I CMGCKG 1 cut(s) 1249
MspCI CTTAAG 1 cut(s) 928
MspI CCGG 4 cut(s) 76, 82, 490, 713
MspR9I CCNGG 5 cut(s) 185, 465, 491, 514, 714
MunI CAATTG 1 cut(s) 1488
Mva1269I GAATGC 1 cut(s) 1125
MvaI CCWGG 3 cut(s) 185, 465, 514
MwoI GCNNNNNNNGC 4 cut(s) 230, 587, 1593, 1742
NciI CCSGG 2 cut(s) 491, 714
NdeI CATATG 1 cut(s) 1592
NheI GCTAGC 3 cut(s) 386, 590, 1385
NlaIII CATG 8 cut(s) 231, 263, 581, 653, 1178, 1286, 1335, 1649
NlaIV GGNNCC 5 cut(s) 80, 662, 894, 1050, 1259
NmuCI GTSAC 1 cut(s) 947
NsiI ATGCAT 1 cut(s) 1738
NspI RCATGY 3 cut(s) 581, 653, 1335
NspV TTCGAA 2 cut(s) 1266, 1547
PagI TCATGA 2 cut(s) 1174, 1645
PceI AGGCCT 1 cut(s) 586
PciI ACATGT 2 cut(s) 649, 1331
PcsI WCGNNNNNNNCGW 3 cut(s) 56, 910, 1354
PctI GAATGC 1 cut(s) 1125
PfeI GAWTC 4 cut(s) 210, 644, 1523, 1687
PflFI GACNNNGTC 3 cut(s) 28, 31, 55
PfoI TCCNGGA 1 cut(s) 712
PkrI GCNGC 6 cut(s) 14, 17, 110, 392, 1262, 1579
PscI ACATGT 2 cut(s) 649, 1331
PsiI TTATAA 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 889
Psp1406I AACGTT 1 cut(s) 986
Psp6I CCWGG 3 cut(s) 183, 463, 512
PspGI CCWGG 3 cut(s) 183, 463, 512
PspN4I GGNNCC 5 cut(s) 80, 662, 894, 1050, 1259
PstI CTGCAG 1 cut(s) 1747
PsuI RGATCY 4 cut(s) 660, 716, 1493, 1727
PsyI GACNNNGTC 3 cut(s) 28, 31, 55
RsaI GTAC 4 cut(s) 699, 957, 1036, 1330
RsaNI GTAC 4 cut(s) 698, 956, 1035, 1329
RseI CAYNNNNRTG 1 cut(s) 576
SacI GAGCTC 1 cut(s) 889
SaqAI TTAA 4 cut(s) 654, 704, 929, 983
SatI GCNGC 6 cut(s) 13, 16, 109, 391, 1261, 1578
ScrFI CCNGG 5 cut(s) 185, 465, 491, 514, 714
SduI GDGCHC 4 cut(s) 352, 889, 1243, 1703
SexAI ACCWGGT 1 cut(s) 183
SfaNI GCATC 3 cut(s) 1342, 1723, 1745
SfcI CTRYAG 2 cut(s) 1723, 1743
SfuI TTCGAA 2 cut(s) 1266, 1547
SmiMI CAYNNNNRTG 1 cut(s) 576
SmlI CTYRAG 2 cut(s) 928, 1679
SmoI CTYRAG 2 cut(s) 928, 1679
Sse9I AATT 7 cut(s) 701, 754, 831, 1198, 1440, 1488, 1538
SseBI AGGCCT 1 cut(s) 586
SsiI CCGC 6 cut(s) 12, 109, 1210, 1249, 1261, 1703
SstI GAGCTC 1 cut(s) 889
StuI AGGCCT 1 cut(s) 586
StyD4I CCNGG 5 cut(s) 183, 463, 489, 512, 712
TaaI ACNGT 2 cut(s) 791, 1376
TaiI ACGT 5 cut(s) 32, 56, 769, 964, 989
TaqI TCGA 8 cut(s) 50, 91, 157, 533, 964, 1266, 1348, 1547
TasI AATT 7 cut(s) 701, 754, 831, 1198, 1440, 1488, 1538
TatI WGTACW 2 cut(s) 697, 1328
TauI GCSGC 3 cut(s) 15, 111, 1263
TfiI GAWTC 4 cut(s) 210, 644, 1523, 1687
Tru1I TTAA 4 cut(s) 654, 704, 929, 983
Tru9I TTAA 4 cut(s) 654, 704, 929, 983
TscAI CASTG 2 cut(s) 370, 1381
TseFI GTSAC 1 cut(s) 947
TseI GCWGC 3 cut(s) 15, 390, 1577
Tsp45I GTSAC 1 cut(s) 947
TspGWI ACGGA 2 cut(s) 919, 1444
TspRI CASTG 2 cut(s) 370, 1381
Tth111I GACNNNGTC 3 cut(s) 28, 31, 55
Vha464I CTTAAG 1 cut(s) 928
VneI GTGCAC 1 cut(s) 1239
XapI RAATTY 4 cut(s) 754, 831, 1198, 1440
XbaI TCTAGA 1 cut(s) 761
XceI RCATGY 3 cut(s) 581, 653, 1335
XcmI CCANNNNNNNNNTGG 1 cut(s) 626
ZraI GACGTC 2 cut(s) 30, 54
Zsp2I ATGCAT 1 cut(s) 1738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.