AT5G45910

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009794
Physical Location & Seq
Reverse (-)
18620299 .. 18622301
2003 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G45910.1

Sequence Viewer

Length: 1119 bp
ATGAGAATCAATATGTTATTCATAGTGGCATTCTCATTTTTAGTCTCTGTTCGGTCATTACCGATGAGACCAACTCTAAAGTACGAGTCAATCTTTAATTTTGGCGATTCTTTAAGCGATACCGGAAACTTTTTATTATCCGGTGATGTTGACTCTCCGAACATTGGAAGACTACCGTACGGACAAACCTTTTTTAACCGTTCCACCGGTCGTTGCTCTGACGGACGTCTCATCATCGATTTCATCGCTGAGGCTAGTGGACTACCGTACATTCCACCGTATCTCCAAAGCTTACGGACGAATGATTCGGTAGATTTCAAGAGAGGTGCAAATTTTGCGGTGGCTGGAGCAACAGCGAACGAATTTAGCTTCTTTAAAAACAGAGGTCTTTCAGTAACATTGTTGACAAACAAGACACTGGATATTCAACTTGATTGGTTCAAGAAGTTGAAACCTTCTCTGTGTAAAACCAAGCCAGAATGTGAACAATATTTTAGAAAATCTCTATTTCTCGTTGGAGAAATTGGTGGAAACGATTATAACTACCCTCTTTTGGCATTCCGAAGTTTCAAACATGCTATGGATTTGGTACCATTTGTTATTAACAAAATCATGGACGTCACAAGTGCATTGATAGAGGAAGGTGCCATGACACTAATAGTTCCAGGAAACCTTCCAATCGGTTGTTCTGCGGCTCTACTAGAGCGGTTTAATGATAATAGTGGATGGCTTTATGACTCGAGGAACCAATGCTACATGCCATTGAACAATTTGGCTAAGCTTCACAATGATAAGCTCAAGAAAGGCCTCGCGGCTCTAAGAAAAAAGTACCCTTATGCCAAAATTATATATGCTGATTATTACAGTTCTGCCATGCAATTCTTCAACTCACCTTCCAAATACGGTTTCACTGGAAGTGTCCTAAAGGCATGTTGTGGAGGAGGAGATGGAAGATACAACGTGCAACCAAACGTGCGGTGCGGAGAAAAGGGTTCAACCACTTGCGAAGATCCATCAACGTACGCAAATTGGGACGGAATTCACCTCACTGAAGCGGCTTACCGCCACATTGCAACTGGTCTCATCTCCGGCCGTTTCACCATGCCTACTTATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

372

Amino Acids

41.53

Weight (kDa)

8.98

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 358 3.2e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 540, 1113
AatII GACGTC 2 cut(s) 229, 621
Acc65I GGTACC 1 cut(s) 589
AccB1I GGYRCC 2 cut(s) 589, 644
AccBSI CCGCTC 1 cut(s) 706
AccII CGCG 1 cut(s) 812
AciI CCGC 8 cut(s) 338, 692, 706, 812, 976, 981, 1055, 1063
AclWI GGATC 1 cut(s) 1004
AcoI YGGCCR 1 cut(s) 1090
AcsI RAATTY 3 cut(s) 331, 362, 1038
AcuI CTGAAG 1 cut(s) 1071
AcyI GRCGYC 2 cut(s) 226, 618
AfaI GTAC 6 cut(s) 83, 179, 269, 591, 830, 1022
AfiI CCNNNNNNNGG 2 cut(s) 164, 553
AgeI ACCGGT 1 cut(s) 206
AgsI TTSAA 8 cut(s) 319, 428, 442, 451, 571, 766, 886, 996
AjnI CCWGG 1 cut(s) 664
AjuI GAANNNNNNNTTGG 4 cut(s) 670, 702, 890, 922
AluBI AGCT 4 cut(s) 291, 369, 781, 796
AluI AGCT 4 cut(s) 291, 369, 781, 796
Alw26I GTCTC 4 cut(s) 49, 61, 233, 1085
AlwI GGATC 1 cut(s) 1004
Ama87I CYCGRG 1 cut(s) 739
AoxI GGCC 2 cut(s) 805, 1090
ApoI RAATTY 3 cut(s) 331, 362, 1038
ArsI GACNNNNNNTTYG 2 cut(s) 289, 321
AsiGI ACCGGT 1 cut(s) 206
Asp718I GGTACC 1 cut(s) 589
AsuHPI GGTGA 4 cut(s) 155, 882, 1034, 1090
AvaI CYCGRG 1 cut(s) 739
BanI GGYRCC 2 cut(s) 589, 644
BbsI GAAGAC 1 cut(s) 175
BbvCI CCTCAGC 1 cut(s) 249
BccI CCATC 3 cut(s) 720, 941, 1021
BceAI ACGGC 1 cut(s) 1077
BciT130I CCWGG 1 cut(s) 666
BcoDI GTCTC 4 cut(s) 49, 61, 233, 1085
BfaI CTAG 2 cut(s) 255, 701
BisI GCNGC 3 cut(s) 693, 813, 1056
BlpI GCTNAGC 1 cut(s) 777
BlsI GCNGC 3 cut(s) 694, 814, 1057
Bme1390I CCNGG 1 cut(s) 666
BmeT110I CYCGRG 1 cut(s) 739
BmiI GGNNCC 3 cut(s) 591, 646, 746
BmrFI CCNGG 1 cut(s) 666
BoxI GACNNNNGTC 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 175
BplI GAGNNNNNCTC 2 cut(s) 58, 90
BpmI CTGGAG 1 cut(s) 366
Bpu10I CCTNAGC 1 cut(s) 249
Bpu1102I GCTNAGC 1 cut(s) 777
BpuEI CTTGAG 1 cut(s) 782
Bsa29I ATCGAT 1 cut(s) 237
BsaHI GRCGYC 2 cut(s) 226, 618
BsaI GGTCTC 2 cut(s) 61, 1085
BsaWI WCCGGW 3 cut(s) 122, 140, 206
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 553
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 3 cut(s) 423, 916, 1081
Bse3DI GCAATG 1 cut(s) 1068
BseBI CCWGG 1 cut(s) 666
BseCI ATCGAT 1 cut(s) 237
BseGI GGATG 1 cut(s) 731
BseLI CCNNNNNNNGG 2 cut(s) 164, 553
BseMI GCAATG 1 cut(s) 1068
BseMII CTCAG 1 cut(s) 240
BseNI ACTGG 3 cut(s) 423, 916, 1081
BseRI GAGGAG 2 cut(s) 954, 957
BseX3I CGGCCG 1 cut(s) 1090
Bsh1236I CGCG 1 cut(s) 812
Bsh1285I CGRYCG 2 cut(s) 211, 1093
BshFI GGCC 2 cut(s) 807, 1092
BshNI GGYRCC 2 cut(s) 589, 644
BshTI ACCGGT 1 cut(s) 206
BshVI ATCGAT 1 cut(s) 237
BsiEI CGRYCG 2 cut(s) 211, 1093
BsiHKCI CYCGRG 1 cut(s) 739
BsiSI CCGG 4 cut(s) 123, 141, 207, 1089
BsiWI CGTACG 2 cut(s) 177, 1020
BslFI GGGAC 1 cut(s) 1046
BslI CCNNNNNNNGG 2 cut(s) 164, 553
BsmAI GTCTC 4 cut(s) 49, 61, 233, 1085
BsmBI CGTCTC 1 cut(s) 233
BsmFI GGGAC 1 cut(s) 1046
BsmI GAATGC 2 cut(s) 29, 557
BsnI GGCC 2 cut(s) 807, 1092
Bso31I GGTCTC 2 cut(s) 61, 1085
BsoBI CYCGRG 1 cut(s) 739
Bsp143I GATC 1 cut(s) 1009
Bsp1720I GCTNAGC 1 cut(s) 777
BspACI CCGC 8 cut(s) 338, 692, 706, 812, 976, 981, 1055, 1063
BspANI GGCC 2 cut(s) 807, 1092
BspCNI CTCAG 1 cut(s) 241
BspDI ATCGAT 1 cut(s) 237
BspFNI CGCG 1 cut(s) 812
BspLI GGNNCC 3 cut(s) 591, 646, 746
BspPI GGATC 1 cut(s) 1004
BspT107I GGYRCC 2 cut(s) 589, 644
BspTNI GGTCTC 2 cut(s) 61, 1085
BsrBI CCGCTC 1 cut(s) 706
BsrDI GCAATG 1 cut(s) 1068
BsrFI RCCGGY 1 cut(s) 206
BsrI ACTGG 3 cut(s) 423, 916, 1081
BssAI RCCGGY 1 cut(s) 206
BssMI GATC 1 cut(s) 1009
BssNI GRCGYC 2 cut(s) 226, 618
Bst2UI CCWGG 1 cut(s) 666
Bst4CI ACNGT 6 cut(s) 177, 200, 267, 279, 866, 905
BstACI GRCGYC 2 cut(s) 226, 618
BstAPI GCANNNNNTGC 1 cut(s) 335
BstDEI CTNAG 3 cut(s) 249, 777, 818
BstF5I GGATG 1 cut(s) 731
BstFNI CGCG 1 cut(s) 812
BstKTI GATC 1 cut(s) 1012
BstMAI GTCTC 4 cut(s) 49, 61, 233, 1085
BstMBI GATC 1 cut(s) 1009
BstMCI CGRYCG 2 cut(s) 211, 1093
BstMWI GCNNNNNNNGC 1 cut(s) 335
BstNI CCWGG 1 cut(s) 666
BstNSI RCATGY 3 cut(s) 578, 760, 933
BstPAI GACNNNNGTC 1 cut(s) 225
BstSCI CCNGG 1 cut(s) 664
BstUI CGCG 1 cut(s) 812
BstV2I GAAGAC 1 cut(s) 175
BstX2I RGATCY 1 cut(s) 1009
BstYI RGATCY 1 cut(s) 1009
BstZI CGGCCG 1 cut(s) 1090
Bsu15I ATCGAT 1 cut(s) 237
BsuRI GGCC 2 cut(s) 807, 1092
BsuTUI ATCGAT 1 cut(s) 237
BtgZI GCGATG 1 cut(s) 229
BtsCI GGATG 1 cut(s) 731
BtsIMutI CAGTG 3 cut(s) 416, 909, 1047
Cfr10I RCCGGY 1 cut(s) 206
ClaI ATCGAT 1 cut(s) 237
Csp6I GTAC 6 cut(s) 82, 178, 268, 590, 829, 1021
CspAI ACCGGT 1 cut(s) 206
CviAII CATG 7 cut(s) 575, 613, 649, 757, 874, 930, 1102
CviQI GTAC 6 cut(s) 82, 178, 268, 590, 829, 1021
DdeI CTNAG 3 cut(s) 249, 777, 818
DpnI GATC 1 cut(s) 1011
DpnII GATC 1 cut(s) 1009
DraI TTTAAA 1 cut(s) 376
EaeI YGGCCR 1 cut(s) 1090
EagI CGGCCG 1 cut(s) 1090
EclXI CGGCCG 1 cut(s) 1090
Eco147I AGGCCT 1 cut(s) 807
Eco31I GGTCTC 2 cut(s) 61, 1085
Eco52I CGGCCG 1 cut(s) 1090
Eco57I CTGAAG 1 cut(s) 1071
Eco88I CYCGRG 1 cut(s) 739
EcoRI GAATTC 1 cut(s) 1038
EcoRII CCWGG 1 cut(s) 664
Esp3I CGTCTC 1 cut(s) 233
FaeI CATG 7 cut(s) 578, 616, 652, 760, 877, 933, 1105
FaqI GGGAC 1 cut(s) 1046
FatI CATG 7 cut(s) 574, 612, 648, 756, 873, 929, 1101
Fnu4HI GCNGC 3 cut(s) 693, 813, 1056
FokI GGATG 1 cut(s) 738
Fsp4HI GCNGC 3 cut(s) 693, 813, 1056
FspBI CTAG 2 cut(s) 255, 701
GluI GCNGC 3 cut(s) 693, 813, 1056
GsuI CTGGAG 1 cut(s) 366
HaeIII GGCC 2 cut(s) 807, 1092
HapII CCGG 4 cut(s) 123, 141, 207, 1089
Hin1I GRCGYC 2 cut(s) 226, 618
Hin1II CATG 7 cut(s) 578, 616, 652, 760, 877, 933, 1105
HincII GTYRAC 2 cut(s) 151, 405
HindII GTYRAC 2 cut(s) 151, 405
HindIII AAGCTT 2 cut(s) 289, 779
HinfI GANTC 6 cut(s) 6, 86, 107, 152, 305, 737
HpaII CCGG 4 cut(s) 123, 141, 207, 1089
HphI GGTGA 4 cut(s) 155, 882, 1034, 1090
Hpy166II GTNNAC 4 cut(s) 151, 260, 405, 485
Hpy188I TCNGA 3 cut(s) 159, 220, 563
Hpy188III TCNNGA 3 cut(s) 319, 442, 799
Hpy8I GTNNAC 4 cut(s) 151, 260, 405, 485
HpyAV CCTTC 4 cut(s) 465, 635, 683, 903
HpyCH4III ACNGT 6 cut(s) 177, 200, 267, 279, 866, 905
HpyCH4IV ACGT 5 cut(s) 226, 618, 960, 972, 1019
HpyCH4V TGCA 5 cut(s) 329, 629, 877, 964, 1073
HpyF10VI GCNNNNNNNGC 1 cut(s) 335
HpyF3I CTNAG 3 cut(s) 249, 777, 818
HpySE526I ACGT 5 cut(s) 226, 618, 960, 972, 1019
Hsp92I GRCGYC 2 cut(s) 226, 618
Hsp92II CATG 7 cut(s) 578, 616, 652, 760, 877, 933, 1105
KpnI GGTACC 1 cut(s) 593
Kzo9I GATC 1 cut(s) 1009
LmnI GCTCC 1 cut(s) 347
MaeI CTAG 2 cut(s) 255, 701
MaeII ACGT 5 cut(s) 226, 618, 960, 972, 1019
MaeIII GTNAC 2 cut(s) 394, 619
MalI GATC 1 cut(s) 1011
MbiI CCGCTC 1 cut(s) 706
MboI GATC 1 cut(s) 1009
MboII GAAGA 4 cut(s) 180, 874, 963, 1019
MflI RGATCY 1 cut(s) 1009
MlyI GAGTC 3 cut(s) 95, 146, 731
MmeI TCCRAC 1 cut(s) 496
MseI TTAA 7 cut(s) 96, 113, 195, 375, 603, 711, 1117
MspI CCGG 4 cut(s) 123, 141, 207, 1089
MspR9I CCNGG 1 cut(s) 666
Mva1269I GAATGC 2 cut(s) 29, 557
MvaI CCWGG 1 cut(s) 666
MvnI CGCG 1 cut(s) 812
MwoI GCNNNNNNNGC 1 cut(s) 335
NdeII GATC 1 cut(s) 1009
NlaIII CATG 7 cut(s) 578, 616, 652, 760, 877, 933, 1105
NlaIV GGNNCC 3 cut(s) 591, 646, 746
NmuCI GTSAC 1 cut(s) 619
NspI RCATGY 3 cut(s) 578, 760, 933
PaeR7I CTCGAG 1 cut(s) 739
PceI AGGCCT 1 cut(s) 807
PctI GAATGC 2 cut(s) 29, 557
PfeI GAWTC 3 cut(s) 6, 107, 305
Pfl23II CGTACG 2 cut(s) 177, 1020
PfoI TCCNGGA 1 cut(s) 664
PinAI ACCGGT 1 cut(s) 206
PkrI GCNGC 3 cut(s) 694, 814, 1057
PleI GAGTC 3 cut(s) 94, 146, 731
PpsI GAGTC 3 cut(s) 94, 146, 731
PshAI GACNNNNGTC 1 cut(s) 225
PsiI TTATAA 2 cut(s) 540, 1113
Psp6I CCWGG 1 cut(s) 664
PspGI CCWGG 1 cut(s) 664
PspLI CGTACG 2 cut(s) 177, 1020
PspN4I GGNNCC 3 cut(s) 591, 646, 746
PspXI VCTCGAGB 1 cut(s) 739
PsrI GAACNNNNNNTAC 2 cut(s) 737, 769
PsuI RGATCY 1 cut(s) 1009
RsaI GTAC 6 cut(s) 83, 179, 269, 591, 830, 1022
RsaNI GTAC 6 cut(s) 82, 178, 268, 590, 829, 1021
SaqAI TTAA 7 cut(s) 96, 113, 195, 375, 603, 711, 1117
SatI GCNGC 3 cut(s) 693, 813, 1056
Sau3AI GATC 1 cut(s) 1009
SchI GAGTC 3 cut(s) 95, 146, 731
ScrFI CCNGG 1 cut(s) 666
Sfr274I CTCGAG 1 cut(s) 739
SlaI CTCGAG 1 cut(s) 739
SmlI CTYRAG 2 cut(s) 739, 797
SmoI CTYRAG 2 cut(s) 739, 797
SseBI AGGCCT 1 cut(s) 807
SsiI CCGC 8 cut(s) 338, 692, 706, 812, 976, 981, 1055, 1063
SspI AATATT 1 cut(s) 491
SspMI CTAG 2 cut(s) 255, 701
StuI AGGCCT 1 cut(s) 807
StyD4I CCNGG 1 cut(s) 664
TaaI ACNGT 6 cut(s) 177, 200, 267, 279, 866, 905
TaiI ACGT 5 cut(s) 229, 621, 963, 975, 1022
TaqI TCGA 2 cut(s) 237, 740
TaqII GACCGA 1 cut(s) 42
TauI GCSGC 3 cut(s) 695, 815, 1058
TfiI GAWTC 3 cut(s) 6, 107, 305
Tru1I TTAA 7 cut(s) 96, 113, 195, 375, 603, 711, 1117
Tru9I TTAA 7 cut(s) 96, 113, 195, 375, 603, 711, 1117
TscAI CASTG 3 cut(s) 423, 916, 1054
TseFI GTSAC 1 cut(s) 619
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 2 cut(s) 10, 232
TspGWI ACGGA 4 cut(s) 195, 237, 310, 1050
TspRI CASTG 3 cut(s) 423, 916, 1054
XapI RAATTY 3 cut(s) 331, 362, 1038
XceI RCATGY 3 cut(s) 578, 760, 933
XcmI CCANNNNNNNNNTGG 1 cut(s) 1073
XhoI CTCGAG 1 cut(s) 739
XspI CTAG 2 cut(s) 255, 701
ZraI GACGTC 2 cut(s) 227, 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.