Rroxscaffold_1G00065960

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0009794
Physical Location & Seq
Reverse (-)
87485880 .. 87488481
2602 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00065960.1

Sequence Viewer

Length: 930 bp
ATGGAGAGACCTTTAACCTTTTTTCACCACGCAACCGGCCGGTTCTCCGATGGCCGCCTTATTGTTGACTTCATCGCTGAGGCATCTGGATTGCCGCATTTACCACCATATTTAGCCCTAAAGAAAGGCCAAAACTTTTACCAAGGAGTAAACTTCGCTGTTGCTGGAGCCACTGCACTTGACCCTGAATTCTTCTACCGAAGGAAAATGGCATCCATTCTGGCCACAAACTACTCCTTGAGCACTCAGCTTGGTTGGTTCAAGGAGCTCAAGCCCTCACTCTGCAACACCAAACAAGAATGTGAGAACTACTTCAAGAAGGCTCTGTTTCTGGTGGGGGAGATAGGTGGAAACGATTACAACTACGCCTTCTTTGTTGGTGGGAGCATTAAACAGCTCAAAGCCTCGGTGCCTCTTGTTGTGGATGCAATTACTAAGGCCACCGGTGCATTGATAGAGGAAGGAGCAGTGGAGCTAGTGGTGCCTGGAAATTTGCCAATTGGGTGCTCAGCCGTCTACTTGACTCTGTATGGAGGCCCTAACAAAGCTTATTATGATAAACGAAATGGGTGTTTGAAGGCGTTCAATGCTTTTGCCAAGCACCATAACAAAGAGCTCAAACGTGCCCTAGGGACACTAGAAGAAAAGTACCCTCATGCAAGGATCATATATGCTGACTACTATGGTGCTTCCATGCCTTTTTATCATGCCCCTCAACATTATGGGTTCACAAGTAGAAGTCTAGCAGCTTGTTGCGGAGGAGGAGGACCATACAACTTCAACAATTCGGCAAGGTGCGGACATATTGGTTCTAAGGCTTGCAAAGATCCATCGTCATATGCAAATTGGGATGGACTTCACTTAACAGAAGCAGCTTATAGCCACATTGCCAAGGGTTTGATCCATGGAAATTTCACCGCACCCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

309

Amino Acids

33.85

Weight (kDa)

8.94

Isoelectric Point (pI)

44.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 10 - 299 2.9e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 409, 481
AccI GTMKAC 1 cut(s) 516
AciI CCGC 5 cut(s) 55, 95, 756, 798, 918
AclWI GGATC 3 cut(s) 671, 821, 895
AcoI YGGCCR 3 cut(s) 37, 52, 222
AcsI RAATTY 3 cut(s) 188, 490, 910
AfaI GTAC 1 cut(s) 650
AgeI ACCGGT 1 cut(s) 443
AgsI TTSAA 5 cut(s) 262, 316, 577, 586, 781
AjnI CCWGG 1 cut(s) 484
AluBI AGCT 8 cut(s) 250, 268, 397, 475, 548, 616, 749, 875
AluI AGCT 8 cut(s) 250, 268, 397, 475, 548, 616, 749, 875
Alw21I GWGCWC 4 cut(s) 245, 270, 509, 618
AlwI GGATC 3 cut(s) 671, 821, 895
AoxI GGCC 6 cut(s) 37, 52, 127, 222, 438, 535
ApeKI GCWGC 2 cut(s) 746, 872
ApoI RAATTY 3 cut(s) 188, 490, 910
AsiGI ACCGGT 1 cut(s) 443
Asp700I GAANNNNTTC 2 cut(s) 311, 581
AspA2I CCTAGG 1 cut(s) 628
AspS9I GGNCC 2 cut(s) 536, 767
AsuHPI GGTGA 2 cut(s) 17, 907
AvaII GGWCC 1 cut(s) 767
AvrII CCTAGG 1 cut(s) 628
BaeGI GKGCMC 1 cut(s) 628
BalI TGGCCA 1 cut(s) 224
BanI GGYRCC 2 cut(s) 409, 481
BanII GRGCYC 2 cut(s) 270, 618
Bbv12I GWGCWC 4 cut(s) 245, 270, 509, 618
BbvCI CCTCAGC 1 cut(s) 78
BbvI GCAGC 2 cut(s) 758, 884
BccI CCATC 3 cut(s) 44, 838, 845
BceAI ACGGC 1 cut(s) 497
BciT130I CCWGG 1 cut(s) 486
BfaI CTAG 5 cut(s) 476, 629, 638, 743, 928
BisI GCNGC 4 cut(s) 55, 95, 747, 873
BlnI CCTAGG 1 cut(s) 628
BlpI GCTNAGC 1 cut(s) 508
BlsI GCNGC 4 cut(s) 56, 96, 748, 874
Bme1390I CCNGG 1 cut(s) 486
Bme18I GGWCC 1 cut(s) 767
BmgT120I GGNCC 2 cut(s) 536, 767
BmiI GGNNCC 3 cut(s) 169, 411, 483
BmrFI CCNGG 1 cut(s) 486
BmsI GCATC 3 cut(s) 92, 221, 415
BpmI CTGGAG 1 cut(s) 186
Bpu10I CCTNAGC 1 cut(s) 78
Bpu1102I GCTNAGC 1 cut(s) 508
BpuEI CTTGAG 2 cut(s) 254, 259
BsaJI CCNNGG 5 cut(s) 142, 405, 628, 891, 904
BsaWI WCCGGW 1 cut(s) 443
Bse118I RCCGGY 3 cut(s) 35, 39, 443
Bse3DI GCAATG 1 cut(s) 885
BseBI CCWGG 1 cut(s) 486
BseDI CCNNGG 5 cut(s) 142, 405, 628, 891, 904
BseGI GGATG 3 cut(s) 212, 430, 856
BseMI GCAATG 1 cut(s) 885
BseMII CTCAG 3 cut(s) 69, 260, 522
BseRI GAGGAG 2 cut(s) 774, 777
BseSI GKGCMC 1 cut(s) 628
BseX3I CGGCCG 1 cut(s) 37
BseXI GCAGC 2 cut(s) 758, 884
BsgI GTGCAG 1 cut(s) 159
Bsh1285I CGRYCG 1 cut(s) 40
BshFI GGCC 6 cut(s) 39, 54, 129, 224, 440, 537
BshNI GGYRCC 2 cut(s) 409, 481
BshTI ACCGGT 1 cut(s) 443
BsiEI CGRYCG 1 cut(s) 40
BsiHKAI GWGCWC 4 cut(s) 245, 270, 509, 618
BsiSI CCGG 3 cut(s) 36, 40, 444
BslFI GGGAC 1 cut(s) 646
BsmFI GGGAC 1 cut(s) 646
BsnI GGCC 6 cut(s) 39, 54, 129, 224, 440, 537
Bsp1286I GDGCHC 5 cut(s) 245, 270, 509, 618, 628
Bsp143I GATC 3 cut(s) 663, 826, 900
Bsp1720I GCTNAGC 1 cut(s) 508
Bsp19I CCATGG 1 cut(s) 904
BspACI CCGC 5 cut(s) 55, 95, 756, 798, 918
BspANI GGCC 6 cut(s) 39, 54, 129, 224, 440, 537
BspCNI CTCAG 3 cut(s) 70, 259, 521
BspLI GGNNCC 3 cut(s) 169, 411, 483
BspPI GGATC 3 cut(s) 671, 821, 895
BspT107I GGYRCC 2 cut(s) 409, 481
BsrDI GCAATG 1 cut(s) 885
BsrFI RCCGGY 3 cut(s) 35, 39, 443
BssAI RCCGGY 3 cut(s) 35, 39, 443
BssECI CCNNGG 5 cut(s) 142, 405, 628, 891, 904
BssMI GATC 3 cut(s) 663, 826, 900
BssT1I CCWWGG 4 cut(s) 142, 628, 891, 904
Bst2UI CCWGG 1 cut(s) 486
BstC8I GCNNGC 1 cut(s) 820
BstDEI CTNAG 5 cut(s) 78, 246, 435, 508, 813
BstDSI CCRYGG 1 cut(s) 904
BstF5I GGATG 3 cut(s) 212, 430, 856
BstKTI GATC 3 cut(s) 666, 829, 903
BstMBI GATC 3 cut(s) 663, 826, 900
BstMCI CGRYCG 1 cut(s) 40
BstMWI GCNNNNNNNGC 3 cut(s) 446, 481, 587
BstNI CCWGG 1 cut(s) 486
BstSCI CCNGG 1 cut(s) 484
BstSLI GKGCMC 1 cut(s) 628
BstV1I GCAGC 2 cut(s) 758, 884
BstX2I RGATCY 1 cut(s) 826
BstYI RGATCY 1 cut(s) 826
BstZI CGGCCG 1 cut(s) 37
BsuRI GGCC 6 cut(s) 39, 54, 129, 224, 440, 537
BtgI CCRYGG 1 cut(s) 904
BtgZI GCGATG 1 cut(s) 58
BtsCI GGATG 3 cut(s) 212, 430, 856
BtsI GCAGTG 2 cut(s) 171, 474
BtsIMutI CAGTG 2 cut(s) 171, 474
Cac8I GCNNGC 1 cut(s) 820
Cfr10I RCCGGY 3 cut(s) 35, 39, 443
Cfr13I GGNCC 2 cut(s) 536, 767
Csp6I GTAC 1 cut(s) 649
CspAI ACCGGT 1 cut(s) 443
CspCI CAANNNNNGTGG 2 cut(s) 160, 195
CviAII CATG 4 cut(s) 656, 694, 707, 905
CviQI GTAC 1 cut(s) 649
DdeI CTNAG 5 cut(s) 78, 246, 435, 508, 813
DpnI GATC 3 cut(s) 665, 828, 902
DpnII GATC 3 cut(s) 663, 826, 900
EaeI YGGCCR 3 cut(s) 37, 52, 222
EagI CGGCCG 1 cut(s) 37
Ecl136II GAGCTC 2 cut(s) 268, 616
EclXI CGGCCG 1 cut(s) 37
Eco130I CCWWGG 4 cut(s) 142, 628, 891, 904
Eco24I GRGCYC 2 cut(s) 270, 618
Eco47I GGWCC 1 cut(s) 767
Eco52I CGGCCG 1 cut(s) 37
Eco53kI GAGCTC 2 cut(s) 268, 616
EcoICRI GAGCTC 2 cut(s) 268, 616
EcoO109I RGGNCCY 1 cut(s) 536
EcoRI GAATTC 1 cut(s) 188
EcoRII CCWGG 1 cut(s) 484
EcoT14I CCWWGG 4 cut(s) 142, 628, 891, 904
EcoT38I GRGCYC 2 cut(s) 270, 618
ErhI CCWWGG 4 cut(s) 142, 628, 891, 904
FaeI CATG 4 cut(s) 659, 697, 710, 908
FaqI GGGAC 1 cut(s) 646
FatI CATG 4 cut(s) 655, 693, 706, 904
FauNDI CATATG 1 cut(s) 838
FblI GTMKAC 1 cut(s) 516
Fnu4HI GCNGC 4 cut(s) 55, 95, 747, 873
FokI GGATG 3 cut(s) 199, 437, 863
FriOI GRGCYC 2 cut(s) 270, 618
Fsp4HI GCNGC 4 cut(s) 55, 95, 747, 873
FspBI CTAG 5 cut(s) 476, 629, 638, 743, 928
GluI GCNGC 4 cut(s) 55, 95, 747, 873
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 6 cut(s) 39, 54, 129, 224, 440, 537
HapII CCGG 3 cut(s) 36, 40, 444
Hin1II CATG 4 cut(s) 659, 697, 710, 908
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HindIII AAGCTT 1 cut(s) 546
HinfI GANTC 1 cut(s) 523
HpaII CCGG 3 cut(s) 36, 40, 444
HphI GGTGA 2 cut(s) 17, 907
Hpy166II GTNNAC 4 cut(s) 67, 151, 517, 729
Hpy188I TCNGA 1 cut(s) 49
Hpy188III TCNNGA 2 cut(s) 87, 316
Hpy8I GTNNAC 4 cut(s) 67, 151, 517, 729
HpyAV CCTTC 5 cut(s) 195, 313, 379, 455, 571
HpyCH4IV ACGT 1 cut(s) 622
HpyCH4V TGCA 7 cut(s) 176, 285, 428, 449, 659, 822, 842
HpyF10VI GCNNNNNNNGC 3 cut(s) 446, 481, 587
HpyF3I CTNAG 5 cut(s) 78, 246, 435, 508, 813
HpySE526I ACGT 1 cut(s) 622
Hsp92II CATG 4 cut(s) 659, 697, 710, 908
Kzo9I GATC 3 cut(s) 663, 826, 900
LmnI GCTCC 5 cut(s) 167, 265, 384, 464, 472
Lsp1109I GCAGC 2 cut(s) 758, 884
LweI GCATC 3 cut(s) 92, 221, 415
MaeI CTAG 5 cut(s) 476, 629, 638, 743, 928
MaeII ACGT 1 cut(s) 622
MalI GATC 3 cut(s) 665, 828, 902
MboI GATC 3 cut(s) 663, 826, 900
MboII GAAGA 2 cut(s) 184, 653
MfeI CAATTG 1 cut(s) 498
MflI RGATCY 1 cut(s) 826
MhlI GDGCHC 5 cut(s) 245, 270, 509, 618, 628
MlsI TGGCCA 1 cut(s) 224
MluCI AATT 7 cut(s) 188, 429, 490, 498, 784, 844, 910
MluNI TGGCCA 1 cut(s) 224
MlyI GAGTC 1 cut(s) 517
Mox20I TGGCCA 1 cut(s) 224
MroXI GAANNNNTTC 2 cut(s) 311, 581
MscI TGGCCA 1 cut(s) 224
MseI TTAA 3 cut(s) 14, 390, 863
Msp20I TGGCCA 1 cut(s) 224
MspI CCGG 3 cut(s) 36, 40, 444
MspR9I CCNGG 1 cut(s) 486
MunI CAATTG 1 cut(s) 498
MvaI CCWGG 1 cut(s) 486
MwoI GCNNNNNNNGC 3 cut(s) 446, 481, 587
NcoI CCATGG 1 cut(s) 904
NdeI CATATG 1 cut(s) 838
NdeII GATC 3 cut(s) 663, 826, 900
NlaIII CATG 4 cut(s) 659, 697, 710, 908
NlaIV GGNNCC 3 cut(s) 169, 411, 483
PdmI GAANNNNTTC 2 cut(s) 311, 581
PinAI ACCGGT 1 cut(s) 443
PkrI GCNGC 4 cut(s) 56, 96, 748, 874
PleI GAGTC 1 cut(s) 517
PpsI GAGTC 1 cut(s) 517
Psp124BI GAGCTC 2 cut(s) 270, 618
Psp6I CCWGG 1 cut(s) 484
PspGI CCWGG 1 cut(s) 484
PspN4I GGNNCC 3 cut(s) 169, 411, 483
PspPI GGNCC 2 cut(s) 536, 767
PsuI RGATCY 1 cut(s) 826
RsaI GTAC 1 cut(s) 650
RsaNI GTAC 1 cut(s) 649
SacI GAGCTC 2 cut(s) 270, 618
SaqAI TTAA 3 cut(s) 14, 390, 863
SatI GCNGC 4 cut(s) 55, 95, 747, 873
Sau3AI GATC 3 cut(s) 663, 826, 900
Sau96I GGNCC 2 cut(s) 536, 767
SchI GAGTC 1 cut(s) 517
ScrFI CCNGG 1 cut(s) 486
SduI GDGCHC 5 cut(s) 245, 270, 509, 618, 628
SfaNI GCATC 3 cut(s) 92, 221, 415
SgrAI CRCCGGYG 1 cut(s) 443
SinI GGWCC 1 cut(s) 767
SmlI CTYRAG 2 cut(s) 238, 269
SmoI CTYRAG 2 cut(s) 238, 269
Sse9I AATT 7 cut(s) 188, 429, 490, 498, 784, 844, 910
SsiI CCGC 5 cut(s) 55, 95, 756, 798, 918
SspMI CTAG 5 cut(s) 476, 629, 638, 743, 928
SstI GAGCTC 2 cut(s) 270, 618
StyD4I CCNGG 1 cut(s) 484
StyI CCWWGG 4 cut(s) 142, 628, 891, 904
TaiI ACGT 1 cut(s) 625
TasI AATT 7 cut(s) 188, 429, 490, 498, 784, 844, 910
TauI GCSGC 2 cut(s) 57, 97
Tru1I TTAA 3 cut(s) 14, 390, 863
Tru9I TTAA 3 cut(s) 14, 390, 863
TscAI CASTG 2 cut(s) 178, 474
TseI GCWGC 2 cut(s) 746, 872
TspDTI ATGAA 1 cut(s) 61
TspRI CASTG 2 cut(s) 178, 474
VpaK11BI GGWCC 1 cut(s) 767
XapI RAATTY 3 cut(s) 188, 490, 910
XmaJI CCTAGG 1 cut(s) 628
XmiI GTMKAC 1 cut(s) 516
XmnI GAANNNNTTC 2 cut(s) 311, 581
XspI CTAG 5 cut(s) 476, 629, 638, 743, 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.