RLG00000031731

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0009794
Physical Location & Seq
Forward (+)
7849695 .. 7851167
1473 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031731

Sequence Viewer

Length: 978 bp
ATGAAGTTGTTCTTCATCTTGCTAGCCTCTTCATGCTTGAGTTTGTACACATTTTTACCAAGTGTTCAATCCTACAAAACGATAATTAACTTTGGTGACTCCATTAGCGACACTGGAAATTACCTTCTTACCGGTGCCTTAAAAAATCCAGTCATCGGAAAGCTCCCTTATGGAATGACCTTTTTTCATCTTCCAACTGGGCGGAGCTCGGATGGACGTGTTCCTGTTGATTTCCTTACTGAGGCATTTGAGTTGCCATTTTTACCACCGTACCAAGCACTGAAGAAAGGCCAAGATTTTTACCATGGAGTGAACTTTGCTGTTGCTGGAGCCACTGCACTTGACCAGAATACTTCACTCTTCCTTGGTTGGTTCAAGAAGCTCAAGTCCTCACTTTGCAACAACAACCAAGAATGCGACAACTACTTCAAGAAGGCTCTGTTTCTGGTGGGGGAGATGGGTGGAAACGATTACAATTACCCCTTTTTCGCTGGTGTGAGAATCAAACAGATCGTAGCTTCAGTACCTCTTCTTGTTGATGCAATCACTAAGGCCACCAGATTCGGTGGAACTTGTCTTCAAATAGTTCATGTTATAGAGATTCTAGATATATGGCATATGGTAACTGCCCAGGATATTTCAACAATGATCCGATGTGGAGAAAATACCATCATATTTCTTATTAACAATGGAGGCTATACAATCGAAGTAGAGATTCGTGATGGCCCATACAATGTCACTAAGAACTGGGATTACACTGGACTCGTTGATGCAATCCACAATGCCGAAGGCAAATGCTGGACTACCAAGGTACGTTCAGAGGAGGACTTAACTGAAGCAATTGCCAAAGCAACAGGGGAGCACAAGGATAGTTATGCTTCATCGAAGTTTTTGTGCACAAAGATGACACTAGCAAAGATCTTCTGGAATGGAAATCCCGAGTTTCAGCTGCCAACAGCCGACCTCCAAATCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

326

Amino Acids

36.24

Weight (kDa)

6.08

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 187 1.6e-12 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 134
AciI CCGC 1 cut(s) 202
AclWI GGATC 1 cut(s) 643
AcuI CTGAAG 3 cut(s) 302, 504, 855
AfaI GTAC 4 cut(s) 47, 272, 525, 813
AfiI CCNNNNNNNGG 2 cut(s) 155, 241
AflIII ACRYGT 1 cut(s) 217
AgeI ACCGGT 1 cut(s) 131
AgsI TTSAA 5 cut(s) 68, 376, 430, 581, 642
AjiI CACGTC 1 cut(s) 218
AjnI CCWGG 1 cut(s) 630
AluBI AGCT 5 cut(s) 163, 207, 382, 518, 949
AluI AGCT 5 cut(s) 163, 207, 382, 518, 949
Alw21I GWGCWC 3 cut(s) 209, 864, 899
Alw44I GTGCAC 1 cut(s) 895
AlwI GGATC 1 cut(s) 643
Ama87I CYCGRG 1 cut(s) 938
AoxI GGCC 3 cut(s) 289, 552, 724
ApaLI GTGCAC 1 cut(s) 895
ApeKI GCWGC 1 cut(s) 949
AsiGI ACCGGT 1 cut(s) 131
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 1 cut(s) 725
AsuHPI GGTGA 1 cut(s) 107
AsuNHI GCTAGC 1 cut(s) 22
AvaI CYCGRG 1 cut(s) 938
BaeGI GKGCMC 1 cut(s) 899
BaeI ACNNNNGTAYC 2 cut(s) 254, 287
BanI GGYRCC 1 cut(s) 134
BanII GRGCYC 1 cut(s) 209
BbsI GAAGAC 1 cut(s) 569
Bbv12I GWGCWC 3 cut(s) 209, 864, 899
BbvI GCAGC 1 cut(s) 936
BccI CCATC 4 cut(s) 206, 451, 677, 716
BciT130I CCWGG 1 cut(s) 632
BfaI CTAG 3 cut(s) 23, 605, 911
BglII AGATCT 1 cut(s) 918
BisI GCNGC 1 cut(s) 950
BlsI GCNGC 1 cut(s) 951
Bme1390I CCNGG 1 cut(s) 632
BmeT110I CYCGRG 1 cut(s) 938
BmgBI CACGTC 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 725
BmiI GGNNCC 2 cut(s) 136, 331
BmrFI CCNGG 1 cut(s) 632
BmrI ACTGGG 2 cut(s) 207, 757
BmsI GCATC 2 cut(s) 529, 760
BmtI GCTAGC 1 cut(s) 26
BmuI ACTGGG 2 cut(s) 207, 757
BpiI GAAGAC 1 cut(s) 569
BpmI CTGGAG 1 cut(s) 348
BpuEI CTTGAG 2 cut(s) 58, 368
BsaJI CCNNGG 4 cut(s) 304, 364, 630, 807
BsaWI WCCGGW 1 cut(s) 131
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 241
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 6 cut(s) 118, 149, 202, 752, 763, 973
BseBI CCWGG 1 cut(s) 632
BseDI CCNNGG 4 cut(s) 304, 364, 630, 807
BseGI GGATG 1 cut(s) 217
BseLI CCNNNNNNNGG 2 cut(s) 155, 241
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 6 cut(s) 118, 149, 202, 752, 763, 973
BseRI GAGGAG 1 cut(s) 836
BseSI GKGCMC 1 cut(s) 899
BseXI GCAGC 1 cut(s) 936
BsgI GTGCAG 1 cut(s) 321
BshFI GGCC 3 cut(s) 291, 554, 726
BshNI GGYRCC 1 cut(s) 134
BshTI ACCGGT 1 cut(s) 131
BsiHKAI GWGCWC 3 cut(s) 209, 864, 899
BsiHKCI CYCGRG 1 cut(s) 938
BsiSI CCGG 1 cut(s) 132
BslI CCNNNNNNNGG 2 cut(s) 155, 241
BsmI GAATGC 1 cut(s) 419
BsnI GGCC 3 cut(s) 291, 554, 726
BsoBI CYCGRG 1 cut(s) 938
Bsp1286I GDGCHC 3 cut(s) 209, 864, 899
Bsp1407I TGTACA 1 cut(s) 45
Bsp143I GATC 3 cut(s) 510, 648, 918
Bsp19I CCATGG 1 cut(s) 304
BspACI CCGC 1 cut(s) 202
BspANI GGCC 3 cut(s) 291, 554, 726
BspCNI CTCAG 1 cut(s) 232
BspLI GGNNCC 2 cut(s) 136, 331
BspOI GCTAGC 1 cut(s) 26
BspPI GGATC 1 cut(s) 643
BspT107I GGYRCC 1 cut(s) 134
BsrFI RCCGGY 1 cut(s) 131
BsrGI TGTACA 1 cut(s) 45
BsrI ACTGG 6 cut(s) 118, 149, 202, 752, 763, 973
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 4 cut(s) 304, 364, 630, 807
BssMI GATC 3 cut(s) 510, 648, 918
BssT1I CCWWGG 3 cut(s) 304, 364, 807
Bst2UI CCWGG 1 cut(s) 632
Bst4CI ACNGT 1 cut(s) 270
Bst6I CTCTTC 3 cut(s) 34, 365, 534
BstAUI TGTACA 1 cut(s) 45
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 3 cut(s) 240, 549, 741
BstDSI CCRYGG 1 cut(s) 304
BstENI CCTNNNNNAGG 1 cut(s) 239
BstF5I GGATG 1 cut(s) 217
BstKTI GATC 3 cut(s) 513, 651, 921
BstMBI GATC 3 cut(s) 510, 648, 918
BstNI CCWGG 1 cut(s) 632
BstSCI CCNGG 1 cut(s) 630
BstSLI GKGCMC 1 cut(s) 899
BstV1I GCAGC 1 cut(s) 936
BstV2I GAAGAC 1 cut(s) 569
BstX2I RGATCY 1 cut(s) 918
BstYI RGATCY 1 cut(s) 918
BsuRI GGCC 3 cut(s) 291, 554, 726
BtgI CCRYGG 1 cut(s) 304
BtrI CACGTC 1 cut(s) 218
BtsCI GGATG 1 cut(s) 217
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 4 cut(s) 111, 278, 333, 756
Cac8I GCNNGC 1 cut(s) 24
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 1 cut(s) 725
Csp6I GTAC 4 cut(s) 46, 271, 524, 812
CspAI ACCGGT 1 cut(s) 131
CspCI CAANNNNNGTGG 2 cut(s) 322, 357
CviAII CATG 3 cut(s) 33, 305, 590
CviQI GTAC 4 cut(s) 46, 271, 524, 812
DdeI CTNAG 3 cut(s) 240, 549, 741
DpnI GATC 3 cut(s) 512, 650, 920
DpnII GATC 3 cut(s) 510, 648, 918
Eam1104I CTCTTC 3 cut(s) 34, 365, 534
EarI CTCTTC 3 cut(s) 34, 365, 534
EciI GGCGGA 1 cut(s) 217
Ecl136II GAGCTC 1 cut(s) 207
Eco130I CCWWGG 3 cut(s) 304, 364, 807
Eco24I GRGCYC 1 cut(s) 209
Eco53kI GAGCTC 1 cut(s) 207
Eco57I CTGAAG 3 cut(s) 302, 504, 855
Eco88I CYCGRG 1 cut(s) 938
EcoICRI GAGCTC 1 cut(s) 207
EcoNI CCTNNNNNAGG 1 cut(s) 239
EcoRII CCWGG 1 cut(s) 630
EcoT14I CCWWGG 3 cut(s) 304, 364, 807
EcoT38I GRGCYC 1 cut(s) 209
ErhI CCWWGG 3 cut(s) 304, 364, 807
FaeI CATG 3 cut(s) 36, 308, 593
FalI AAGNNNNNCTT 1 cut(s) 28
FatI CATG 3 cut(s) 32, 304, 589
FauNDI CATATG 1 cut(s) 618
Fnu4HI GCNGC 1 cut(s) 950
FokI GGATG 1 cut(s) 224
FriOI GRGCYC 1 cut(s) 209
Fsp4HI GCNGC 1 cut(s) 950
FspBI CTAG 3 cut(s) 23, 605, 911
GluI GCNGC 1 cut(s) 950
GsuI CTGGAG 1 cut(s) 348
HaeIII GGCC 3 cut(s) 291, 554, 726
HapII CCGG 1 cut(s) 132
Hin1II CATG 3 cut(s) 36, 308, 593
HinfI GANTC 6 cut(s) 98, 501, 561, 601, 715, 762
HpaII CCGG 1 cut(s) 132
HphI GGTGA 1 cut(s) 107
Hpy166II GTNNAC 3 cut(s) 48, 313, 897
Hpy188I TCNGA 4 cut(s) 158, 211, 653, 820
Hpy188III TCNNGA 6 cut(s) 376, 430, 605, 719, 925, 938
Hpy8I GTNNAC 3 cut(s) 48, 313, 897
HpyAV CCTTC 3 cut(s) 134, 427, 782
HpyCH4III ACNGT 1 cut(s) 270
HpyCH4IV ACGT 2 cut(s) 217, 814
HpyCH4V TGCA 5 cut(s) 338, 399, 542, 773, 897
HpyF3I CTNAG 3 cut(s) 240, 549, 741
HpySE526I ACGT 2 cut(s) 217, 814
Hsp92II CATG 3 cut(s) 36, 308, 593
Kzo9I GATC 3 cut(s) 510, 648, 918
LmnI GCTCC 4 cut(s) 168, 204, 329, 859
Lsp1109I GCAGC 1 cut(s) 936
LweI GCATC 2 cut(s) 529, 760
MaeI CTAG 3 cut(s) 23, 605, 911
MaeII ACGT 2 cut(s) 217, 814
MaeIII GTNAC 3 cut(s) 95, 622, 736
MalI GATC 3 cut(s) 512, 650, 920
MboI GATC 3 cut(s) 510, 648, 918
MboII GAAGA 8 cut(s) 4, 21, 182, 295, 352, 521, 569, 913
MfeI CAATTG 1 cut(s) 840
MflI RGATCY 1 cut(s) 918
MhlI GDGCHC 3 cut(s) 209, 864, 899
MluCI AATT 4 cut(s) 84, 118, 475, 840
MlyI GAGTC 2 cut(s) 92, 756
MmeI TCCRAC 1 cut(s) 218
MnlI CCTC 8 cut(s) 37, 235, 400, 537, 686, 814, 817, 974
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 4 cut(s) 87, 140, 684, 830
MslI CAYNNNNRTG 1 cut(s) 902
MspA1I CMGCKG 1 cut(s) 949
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 1 cut(s) 632
MunI CAATTG 1 cut(s) 840
Mva1269I GAATGC 1 cut(s) 419
MvaI CCWGG 1 cut(s) 632
NcoI CCATGG 1 cut(s) 304
NdeI CATATG 1 cut(s) 618
NdeII GATC 3 cut(s) 510, 648, 918
NheI GCTAGC 1 cut(s) 22
NlaIII CATG 3 cut(s) 36, 308, 593
NlaIV GGNNCC 2 cut(s) 136, 331
NmuCI GTSAC 2 cut(s) 95, 736
PctI GAATGC 1 cut(s) 419
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 4 cut(s) 501, 561, 601, 715
PinAI ACCGGT 1 cut(s) 131
PkrI GCNGC 1 cut(s) 951
PleI GAGTC 2 cut(s) 92, 756
PpsI GAGTC 2 cut(s) 92, 756
Psp124BI GAGCTC 1 cut(s) 209
Psp6I CCWGG 1 cut(s) 630
PspGI CCWGG 1 cut(s) 630
PspN4I GGNNCC 2 cut(s) 136, 331
PspPI GGNCC 1 cut(s) 725
PsrI GAACNNNNNNTAC 2 cut(s) 737, 769
PsuI RGATCY 1 cut(s) 918
PvuII CAGCTG 1 cut(s) 949
RsaI GTAC 4 cut(s) 47, 272, 525, 813
RsaNI GTAC 4 cut(s) 46, 271, 524, 812
RseI CAYNNNNRTG 1 cut(s) 902
SacI GAGCTC 1 cut(s) 209
SaqAI TTAA 4 cut(s) 87, 140, 684, 830
SatI GCNGC 1 cut(s) 950
Sau3AI GATC 3 cut(s) 510, 648, 918
Sau96I GGNCC 1 cut(s) 725
SchI GAGTC 2 cut(s) 92, 756
ScrFI CCNGG 1 cut(s) 632
SduI GDGCHC 3 cut(s) 209, 864, 899
SfaNI GCATC 2 cut(s) 529, 760
SmiMI CAYNNNNRTG 1 cut(s) 902
SmlI CTYRAG 2 cut(s) 37, 383
SmoI CTYRAG 2 cut(s) 37, 383
Sse9I AATT 4 cut(s) 84, 118, 475, 840
SsiI CCGC 1 cut(s) 202
SspMI CTAG 3 cut(s) 23, 605, 911
SstI GAGCTC 1 cut(s) 209
StyD4I CCNGG 1 cut(s) 630
StyI CCWWGG 3 cut(s) 304, 364, 807
TaaI ACNGT 1 cut(s) 270
TaiI ACGT 2 cut(s) 220, 817
TaqI TCGA 2 cut(s) 705, 884
TasI AATT 4 cut(s) 84, 118, 475, 840
TatI WGTACW 1 cut(s) 45
TfiI GAWTC 4 cut(s) 501, 561, 601, 715
Tru1I TTAA 4 cut(s) 87, 140, 684, 830
Tru9I TTAA 4 cut(s) 87, 140, 684, 830
TscAI CASTG 4 cut(s) 118, 285, 340, 763
TseFI GTSAC 2 cut(s) 95, 736
TseI GCWGC 1 cut(s) 949
Tsp45I GTSAC 2 cut(s) 95, 736
TspDTI ATGAA 6 cut(s) 4, 17, 21, 176, 578, 870
TspRI CASTG 4 cut(s) 118, 285, 340, 763
VneI GTGCAC 1 cut(s) 895
XagI CCTNNNNNAGG 1 cut(s) 239
XbaI TCTAGA 1 cut(s) 604
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 3 cut(s) 23, 605, 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.