Rmu_sc0000818.1_g000017

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000818.1
OG0009794
Physical Location & Seq
Forward (+)
82833 .. 84808
1976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000818.1_g000017.1.cds

Sequence Viewer

Length: 1092 bp
atgaagctgtttatcctgctagcttctttgtgcttgactttgcacgtagtctcatcaagtcttcaacagtatcaagcaatattcaacttcggtgactccctcagtgacaccggaaatttccttctctccgatccccgcgcacccccaaacgtcattggtaagctaccttatggagagaccttttttcaccacgcaaccggccggttctcggatggccgccttattgttgacttcatcgctgaggcatctggattgccgcatttaccaccatatttagccctaaagaaaggccaaaacttttaccaaggagtaaactttgctgttgctggagccactgcacttgaccctgaattcttctaccgaaggaaaatggcatcgattctggccacaaactactccttgagcactcagcttggttggttcaaggagctcaagccctcactctgcaacaccaaacaagaatgcgagaactacttcaagaaggctctgtttctggtgggggagataggtggaaacgattacaactacgccttctttgttggtgggagcattaaacagctcaaagcctcggtgcctcttgttgtggatgcaattactaaggccaccggtgcattgatagaggaaggagcagtggagctagtggtgcctggaaatttgccaattgggtgctcagccgcctacttgactctgtatggaggccctaacaaagcttattatgataaacgaaatgggtgtttgaaggcgttcaatgcttttgccaagcaccataacaaagagctcaaacgtgccctagggacactagaagaaaaataccctcatgcaaggatcatatatgctgactactatggtgcttccatgcctttttatcatgcccctcaacattatgggtttacaagtggaagtctagcagcttgttgcggaggaggaggaccatacaacttcaacaattcggcatggtgtggacatattggttctaaggcttgcaaagatccatcgtcatatgcaaattgggatggacttcacttaacagaagcagcttatagccacattgccaagggtttgatccatggaaatttcaccgcacccatctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

363

Amino Acids

39.55

Weight (kDa)

8.57

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 571, 643
AccII CGCG 1 cut(s) 138
AciI CCGC 6 cut(s) 136, 217, 257, 675, 918, 1080
AclWI GGATC 4 cut(s) 125, 833, 983, 1057
AcoI YGGCCR 3 cut(s) 199, 214, 384
AcsI RAATTY 4 cut(s) 115, 350, 652, 1072
AfiI CCNNNNNNNGG 1 cut(s) 208
AgeI ACCGGT 1 cut(s) 605
AgsI TTSAA 7 cut(s) 65, 85, 424, 478, 739, 748, 943
AjnI CCWGG 1 cut(s) 646
Alw21I GWGCWC 4 cut(s) 407, 432, 671, 780
Alw26I GTCTC 2 cut(s) 55, 170
AlwI GGATC 4 cut(s) 125, 833, 983, 1057
AoxI GGCC 6 cut(s) 199, 214, 289, 384, 600, 697
ApeKI GCWGC 2 cut(s) 908, 1034
ApoI RAATTY 4 cut(s) 115, 350, 652, 1072
AsiGI ACCGGT 1 cut(s) 605
Asp700I GAANNNNTTC 2 cut(s) 473, 743
AspA2I CCTAGG 1 cut(s) 790
AspLEI GCGC 1 cut(s) 140
AspS9I GGNCC 2 cut(s) 698, 929
AsuHPI GGTGA 3 cut(s) 104, 179, 1069
AsuNHI GCTAGC 1 cut(s) 19
AvaII GGWCC 1 cut(s) 929
AvrII CCTAGG 1 cut(s) 790
BaeGI GKGCMC 1 cut(s) 790
BalI TGGCCA 1 cut(s) 386
BanI GGYRCC 2 cut(s) 571, 643
BanII GRGCYC 2 cut(s) 432, 780
BbsI GAAGAC 1 cut(s) 53
Bbv12I GWGCWC 4 cut(s) 407, 432, 671, 780
BbvCI CCTCAGC 1 cut(s) 240
BbvI GCAGC 2 cut(s) 920, 1046
BccI CCATC 3 cut(s) 206, 1000, 1007
BciT130I CCWGG 1 cut(s) 648
BcoDI GTCTC 2 cut(s) 55, 170
BfaI CTAG 6 cut(s) 20, 638, 791, 800, 905, 1090
BisI GCNGC 5 cut(s) 217, 257, 675, 909, 1035
BlnI CCTAGG 1 cut(s) 790
BlpI GCTNAGC 1 cut(s) 670
BlsI GCNGC 5 cut(s) 218, 258, 676, 910, 1036
Bme1390I CCNGG 1 cut(s) 648
Bme18I GGWCC 1 cut(s) 929
BmgT120I GGNCC 2 cut(s) 698, 929
BmiI GGNNCC 3 cut(s) 331, 573, 645
BmrFI CCNGG 1 cut(s) 648
BmsI GCATC 3 cut(s) 254, 383, 577
BmtI GCTAGC 1 cut(s) 23
BpiI GAAGAC 1 cut(s) 53
BpmI CTGGAG 1 cut(s) 348
Bpu10I CCTNAGC 1 cut(s) 240
Bpu1102I GCTNAGC 1 cut(s) 670
BpuEI CTTGAG 2 cut(s) 416, 421
Bsa29I ATCGAT 1 cut(s) 377
BsaAI YACGTR 1 cut(s) 46
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 5 cut(s) 304, 567, 790, 1053, 1066
BsaWI WCCGGW 2 cut(s) 110, 605
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse118I RCCGGY 3 cut(s) 197, 201, 605
Bse3DI GCAATG 1 cut(s) 1047
BseBI CCWGG 1 cut(s) 648
BseCI ATCGAT 1 cut(s) 377
BseDI CCNNGG 5 cut(s) 304, 567, 790, 1053, 1066
BseGI GGATG 3 cut(s) 217, 592, 1018
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMI GCAATG 1 cut(s) 1047
BseMII CTCAG 4 cut(s) 115, 231, 422, 684
BseRI GAGGAG 2 cut(s) 936, 939
BseSI GKGCMC 1 cut(s) 790
BseX3I CGGCCG 1 cut(s) 199
BseXI GCAGC 2 cut(s) 920, 1046
BsgI GTGCAG 1 cut(s) 321
Bsh1236I CGCG 1 cut(s) 138
Bsh1285I CGRYCG 1 cut(s) 202
BshFI GGCC 6 cut(s) 201, 216, 291, 386, 602, 699
BshNI GGYRCC 2 cut(s) 571, 643
BshTI ACCGGT 1 cut(s) 605
BshVI ATCGAT 1 cut(s) 377
BsiEI CGRYCG 1 cut(s) 202
BsiHKAI GWGCWC 4 cut(s) 407, 432, 671, 780
BsiSI CCGG 4 cut(s) 111, 198, 202, 606
BslFI GGGAC 1 cut(s) 808
BslI CCNNNNNNNGG 1 cut(s) 208
BsmAI GTCTC 2 cut(s) 55, 170
BsmFI GGGAC 1 cut(s) 808
BsmI GAATGC 1 cut(s) 467
BsnI GGCC 6 cut(s) 201, 216, 291, 386, 602, 699
Bso31I GGTCTC 1 cut(s) 170
Bsp1286I GDGCHC 5 cut(s) 407, 432, 671, 780, 790
Bsp143I GATC 4 cut(s) 130, 825, 988, 1062
Bsp1720I GCTNAGC 1 cut(s) 670
Bsp19I CCATGG 1 cut(s) 1066
BspACI CCGC 6 cut(s) 136, 217, 257, 675, 918, 1080
BspANI GGCC 6 cut(s) 201, 216, 291, 386, 602, 699
BspCNI CTCAG 4 cut(s) 114, 232, 421, 683
BspDI ATCGAT 1 cut(s) 377
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 3 cut(s) 331, 573, 645
BspOI GCTAGC 1 cut(s) 23
BspPI GGATC 4 cut(s) 125, 833, 983, 1057
BspT107I GGYRCC 2 cut(s) 571, 643
BspTNI GGTCTC 1 cut(s) 170
BsrDI GCAATG 1 cut(s) 1047
BsrFI RCCGGY 3 cut(s) 197, 201, 605
BssAI RCCGGY 3 cut(s) 197, 201, 605
BssECI CCNNGG 5 cut(s) 304, 567, 790, 1053, 1066
BssMI GATC 4 cut(s) 130, 825, 988, 1062
BssT1I CCWWGG 4 cut(s) 304, 790, 1053, 1066
Bst2UI CCWGG 1 cut(s) 648
Bst4CI ACNGT 1 cut(s) 69
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 2 cut(s) 21, 982
BstDEI CTNAG 6 cut(s) 101, 240, 408, 597, 670, 975
BstDSI CCRYGG 1 cut(s) 1066
BstF5I GGATG 3 cut(s) 217, 592, 1018
BstFNI CGCG 1 cut(s) 138
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 4 cut(s) 133, 828, 991, 1065
BstMAI GTCTC 2 cut(s) 55, 170
BstMBI GATC 4 cut(s) 130, 825, 988, 1062
BstMCI CGRYCG 1 cut(s) 202
BstMWI GCNNNNNNNGC 3 cut(s) 608, 643, 749
BstNI CCWGG 1 cut(s) 648
BstSCI CCNGG 1 cut(s) 646
BstSLI GKGCMC 1 cut(s) 790
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 2 cut(s) 920, 1046
BstV2I GAAGAC 1 cut(s) 53
BstX2I RGATCY 1 cut(s) 988
BstYI RGATCY 1 cut(s) 988
BstZI CGGCCG 1 cut(s) 199
Bsu15I ATCGAT 1 cut(s) 377
BsuRI GGCC 6 cut(s) 201, 216, 291, 386, 602, 699
BsuTUI ATCGAT 1 cut(s) 377
BtgI CCRYGG 1 cut(s) 1066
BtgZI GCGATG 1 cut(s) 220
BtsCI GGATG 3 cut(s) 217, 592, 1018
BtsI GCAGTG 2 cut(s) 333, 636
BtsIMutI CAGTG 3 cut(s) 109, 333, 636
Cac8I GCNNGC 2 cut(s) 21, 982
CfoI GCGC 1 cut(s) 140
Cfr10I RCCGGY 3 cut(s) 197, 201, 605
Cfr13I GGNCC 2 cut(s) 698, 929
ClaI ATCGAT 1 cut(s) 377
CspAI ACCGGT 1 cut(s) 605
CspCI CAANNNNNGTGG 2 cut(s) 322, 357
CviAII CATG 5 cut(s) 818, 856, 869, 954, 1067
DdeI CTNAG 6 cut(s) 101, 240, 408, 597, 670, 975
DpnI GATC 4 cut(s) 132, 827, 990, 1064
DpnII GATC 4 cut(s) 130, 825, 988, 1062
EaeI YGGCCR 3 cut(s) 199, 214, 384
EagI CGGCCG 1 cut(s) 199
Ecl136II GAGCTC 2 cut(s) 430, 778
EclXI CGGCCG 1 cut(s) 199
Eco130I CCWWGG 4 cut(s) 304, 790, 1053, 1066
Eco24I GRGCYC 2 cut(s) 432, 780
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 1 cut(s) 929
Eco52I CGGCCG 1 cut(s) 199
Eco53kI GAGCTC 2 cut(s) 430, 778
EcoICRI GAGCTC 2 cut(s) 430, 778
EcoO109I RGGNCCY 1 cut(s) 698
EcoRI GAATTC 1 cut(s) 350
EcoRII CCWGG 1 cut(s) 646
EcoT14I CCWWGG 4 cut(s) 304, 790, 1053, 1066
EcoT38I GRGCYC 2 cut(s) 432, 780
ErhI CCWWGG 4 cut(s) 304, 790, 1053, 1066
FaeI CATG 5 cut(s) 821, 859, 872, 957, 1070
FaqI GGGAC 1 cut(s) 808
FatI CATG 5 cut(s) 817, 855, 868, 953, 1066
FauI CCCGC 1 cut(s) 143
FauNDI CATATG 1 cut(s) 1000
Fnu4HI GCNGC 5 cut(s) 217, 257, 675, 909, 1035
FokI GGATG 3 cut(s) 224, 599, 1025
FriOI GRGCYC 2 cut(s) 432, 780
Fsp4HI GCNGC 5 cut(s) 217, 257, 675, 909, 1035
FspBI CTAG 6 cut(s) 20, 638, 791, 800, 905, 1090
GlaI GCGC 1 cut(s) 139
GluI GCNGC 5 cut(s) 217, 257, 675, 909, 1035
GsuI CTGGAG 1 cut(s) 348
HaeIII GGCC 6 cut(s) 201, 216, 291, 386, 602, 699
HapII CCGG 4 cut(s) 111, 198, 202, 606
HhaI GCGC 1 cut(s) 140
Hin1II CATG 5 cut(s) 821, 859, 872, 957, 1070
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HindIII AAGCTT 1 cut(s) 708
HinfI GANTC 3 cut(s) 95, 379, 685
HpaII CCGG 4 cut(s) 111, 198, 202, 606
HphI GGTGA 3 cut(s) 104, 179, 1069
Hpy166II GTNNAC 4 cut(s) 229, 313, 891, 962
Hpy188I TCNGA 2 cut(s) 130, 211
Hpy188III TCNNGA 2 cut(s) 249, 478
Hpy8I GTNNAC 4 cut(s) 229, 313, 891, 962
HpyAV CCTTC 6 cut(s) 131, 357, 475, 541, 617, 733
HpyCH4III ACNGT 1 cut(s) 69
HpyCH4IV ACGT 3 cut(s) 45, 150, 784
HpyCH4V TGCA 8 cut(s) 43, 338, 447, 590, 611, 821, 984, 1004
HpyF10VI GCNNNNNNNGC 3 cut(s) 608, 643, 749
HpyF3I CTNAG 6 cut(s) 101, 240, 408, 597, 670, 975
HpySE526I ACGT 3 cut(s) 45, 150, 784
Hsp92II CATG 5 cut(s) 821, 859, 872, 957, 1070
HspAI GCGC 1 cut(s) 138
Kzo9I GATC 4 cut(s) 130, 825, 988, 1062
LmnI GCTCC 5 cut(s) 329, 427, 546, 626, 634
Lsp1109I GCAGC 2 cut(s) 920, 1046
LweI GCATC 3 cut(s) 254, 383, 577
MaeI CTAG 6 cut(s) 20, 638, 791, 800, 905, 1090
MaeII ACGT 3 cut(s) 45, 150, 784
MaeIII GTNAC 2 cut(s) 92, 104
MalI GATC 4 cut(s) 132, 827, 990, 1064
MboI GATC 4 cut(s) 130, 825, 988, 1062
MboII GAAGA 3 cut(s) 53, 346, 815
MfeI CAATTG 1 cut(s) 660
MflI RGATCY 1 cut(s) 988
MhlI GDGCHC 5 cut(s) 407, 432, 671, 780, 790
MlsI TGGCCA 1 cut(s) 386
MluCI AATT 8 cut(s) 115, 350, 591, 652, 660, 946, 1006, 1072
MluNI TGGCCA 1 cut(s) 386
MlyI GAGTC 2 cut(s) 89, 679
Mox20I TGGCCA 1 cut(s) 386
MroXI GAANNNNTTC 2 cut(s) 473, 743
MscI TGGCCA 1 cut(s) 386
MseI TTAA 2 cut(s) 552, 1025
Msp20I TGGCCA 1 cut(s) 386
MspI CCGG 4 cut(s) 111, 198, 202, 606
MspR9I CCNGG 1 cut(s) 648
MunI CAATTG 1 cut(s) 660
Mva1269I GAATGC 1 cut(s) 467
MvaI CCWGG 1 cut(s) 648
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 3 cut(s) 608, 643, 749
NcoI CCATGG 1 cut(s) 1066
NdeI CATATG 1 cut(s) 1000
NdeII GATC 4 cut(s) 130, 825, 988, 1062
NheI GCTAGC 1 cut(s) 19
NlaIII CATG 5 cut(s) 821, 859, 872, 957, 1070
NlaIV GGNNCC 3 cut(s) 331, 573, 645
NmuCI GTSAC 2 cut(s) 92, 104
PctI GAATGC 1 cut(s) 467
PdmI GAANNNNTTC 2 cut(s) 473, 743
PfeI GAWTC 1 cut(s) 379
PinAI ACCGGT 1 cut(s) 605
PkrI GCNGC 5 cut(s) 218, 258, 676, 910, 1036
PleI GAGTC 2 cut(s) 89, 679
PpsI GAGTC 2 cut(s) 89, 679
Ppu21I YACGTR 1 cut(s) 46
Psp124BI GAGCTC 2 cut(s) 432, 780
Psp6I CCWGG 1 cut(s) 646
PspGI CCWGG 1 cut(s) 646
PspN4I GGNNCC 3 cut(s) 331, 573, 645
PspPI GGNCC 2 cut(s) 698, 929
PsuI RGATCY 1 cut(s) 988
SacI GAGCTC 2 cut(s) 432, 780
SaqAI TTAA 2 cut(s) 552, 1025
SatI GCNGC 5 cut(s) 217, 257, 675, 909, 1035
Sau3AI GATC 4 cut(s) 130, 825, 988, 1062
Sau96I GGNCC 2 cut(s) 698, 929
SchI GAGTC 2 cut(s) 89, 679
ScrFI CCNGG 1 cut(s) 648
SduI GDGCHC 5 cut(s) 407, 432, 671, 780, 790
SfaNI GCATC 3 cut(s) 254, 383, 577
SgrAI CRCCGGYG 1 cut(s) 605
SinI GGWCC 1 cut(s) 929
SmlI CTYRAG 2 cut(s) 400, 431
SmoI CTYRAG 2 cut(s) 400, 431
Sse9I AATT 8 cut(s) 115, 350, 591, 652, 660, 946, 1006, 1072
SsiI CCGC 6 cut(s) 136, 217, 257, 675, 918, 1080
SspI AATATT 1 cut(s) 81
SspMI CTAG 6 cut(s) 20, 638, 791, 800, 905, 1090
SstI GAGCTC 2 cut(s) 432, 780
StyD4I CCNGG 1 cut(s) 646
StyI CCWWGG 4 cut(s) 304, 790, 1053, 1066
TaaI ACNGT 1 cut(s) 69
TaiI ACGT 3 cut(s) 48, 153, 787
TaqI TCGA 1 cut(s) 377
TasI AATT 8 cut(s) 115, 350, 591, 652, 660, 946, 1006, 1072
TauI GCSGC 3 cut(s) 219, 259, 677
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 2 cut(s) 552, 1025
Tru9I TTAA 2 cut(s) 552, 1025
TscAI CASTG 3 cut(s) 109, 340, 636
TseFI GTSAC 2 cut(s) 92, 104
TseI GCWGC 2 cut(s) 908, 1034
Tsp45I GTSAC 2 cut(s) 92, 104
TspDTI ATGAA 2 cut(s) 17, 223
TspRI CASTG 3 cut(s) 109, 340, 636
VpaK11BI GGWCC 1 cut(s) 929
XapI RAATTY 4 cut(s) 115, 350, 652, 1072
XmaJI CCTAGG 1 cut(s) 790
XmnI GAANNNNTTC 2 cut(s) 473, 743
XspI CTAG 6 cut(s) 20, 638, 791, 800, 905, 1090
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.