MD10G1283000.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009794
Physical Location & Seq
Reverse (-)
37341540 .. 37343514
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1283000.v1.1.491

Sequence Viewer

Length: 1104 bp
ATGAAGTTGATTAATATCCTTGTAGTCTCCTCTTTATGCCTCACGTTGTGTTTGAGCACTCTAGTCTCATCAAGTCTTCAACCCTACCAGGCAATATTCAACTTCGGCGACTCCCTCAGCGACACCGGAAATTTCCTTCTCTCCGGTGCCCTTGCATTTCCCGTCATCGGAAAGCTCCCTTATGGAGAGACCTTTTTTCAACACGCAACCGGTCGCTGCTCCGATGGACGGCTTGTCGTCGATTTCATTGTTGAGGCATTTGGGTTGCCACTTTTGCCACCCTACCTAGCACTGAACAAAACCCAAGATATTCAGCATGGAGTGAACTTTGCTGTTGCTGGAGCCACTGCACTTGACCCAGAATTCTTCTACCAAAGAAAAATCGGACCAGTTATGTGGACGAATGACTCTTTGAGCACTCAGCTTGGTTGGTTCAAGAAGCTGAAGCCATCACTTTGCAGAACCAAACAAGAGTGTGACAACTACTTCAAGAAGACTCTCTTTTTGGTGGGGGAGATAGGTGGAAACGATTACAATTACGCTTTCTTTGTCGGTGGCAACATGGAACAGCTCAAAGCTTCAGTACCATACGTTGTTGGAGCAATTACTAACGCCACCAGTGCATTGATAGAAGAAGGAGCAGTGGAGTTGTTGGTGCCTGGAAACTTGCCAATTGGATGTTCAGCCGTGTATTTGACTTTGTTTCAAACCACTAACAGAGCTTATTATGATAAACGAAATGGGTGTTTGAAGGCTTTCAATGCTTTCTCCAAATACCATAACAGTGAGCTCAAACGTGCCCTAGCAACACTTAGACAGAAATACCCTCATGCAAATATAATATATGCTGACTACTATGGCGCAGCCATGCCCTTTTTCCATGCCCCTCAACATTATGGATTCAAAAGTGGGACTCTAAGGGCATGCTGTGGAGGAGGAGGGCCATACAATTTCAATAATTCAGCAAGGTGTGGACACATAGGATCAACTGCCTGTAAAGATCCATCTTCCTATGTAAACTGGGATGGAATTCACTTAACAGAAGCTGCTTATCGCTACATTGCCGAGGGCTTGATCCATGGCCAGTTTACAAAACCCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

368

Amino Acids

40.3

Weight (kDa)

8.52

Isoelectric Point (pI)

36.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 32 - 357 9.2e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 146, 655
AclWI GGATC 3 cut(s) 991, 995, 1069
AcoI YGGCCR 1 cut(s) 1081
AcsI RAATTY 3 cut(s) 130, 362, 1029
AcuI CTGAAG 2 cut(s) 464, 564
AdeI CACNNNGTG 1 cut(s) 48
AfaI GTAC 1 cut(s) 585
AfiI CCNNNNNNNGG 2 cut(s) 167, 228
AgeI ACCGGT 1 cut(s) 209
AhdI GACNNNNNGTC 1 cut(s) 233
AjnI CCWGG 2 cut(s) 87, 658
AjuI GAANNNNNNNTTGG 2 cut(s) 664, 696
AluBI AGCT 8 cut(s) 175, 424, 442, 571, 578, 722, 790, 1046
AluI AGCT 8 cut(s) 175, 424, 442, 571, 578, 722, 790, 1046
Alw21I GWGCWC 3 cut(s) 59, 419, 792
Alw26I GTCTC 3 cut(s) 31, 70, 182
AlwI GGATC 3 cut(s) 991, 995, 1069
AlwNI CAGNNNCTG 1 cut(s) 1046
AoxI GGCC 2 cut(s) 941, 1081
ApeKI GCWGC 3 cut(s) 216, 863, 1046
ApoI RAATTY 3 cut(s) 130, 362, 1029
ArsI GACNNNNNNTTYG 2 cut(s) 487, 519
AseI ATTAAT 1 cut(s) 12
AsiGI ACCGGT 1 cut(s) 209
Asp700I GAANNNNTTC 1 cut(s) 755
AspLEI GCGC 1 cut(s) 863
AspS9I GGNCC 2 cut(s) 386, 941
AvaII GGWCC 1 cut(s) 386
BaeGI GKGCMC 2 cut(s) 151, 802
BalI TGGCCA 1 cut(s) 1083
BanI GGYRCC 2 cut(s) 146, 655
BanII GRGCYC 1 cut(s) 792
BbsI GAAGAC 2 cut(s) 68, 500
Bbv12I GWGCWC 3 cut(s) 59, 419, 792
BbvCI CCTCAGC 1 cut(s) 116
BbvI GCAGC 3 cut(s) 203, 875, 1033
BccI CCATC 4 cut(s) 218, 457, 1012, 1019
BceAI ACGGC 2 cut(s) 245, 671
BciT130I CCWGG 2 cut(s) 89, 660
BcoDI GTCTC 3 cut(s) 31, 70, 182
BfaI CTAG 3 cut(s) 62, 287, 803
BisI GCNGC 3 cut(s) 217, 864, 1047
BlsI GCNGC 3 cut(s) 218, 865, 1048
Bme1390I CCNGG 2 cut(s) 89, 660
Bme18I GGWCC 1 cut(s) 386
BmeRI GACNNNNNGTC 1 cut(s) 233
BmgT120I GGNCC 2 cut(s) 386, 941
BmiI GGNNCC 3 cut(s) 148, 343, 657
BmrFI CCNGG 2 cut(s) 89, 660
BmrI ACTGGG 1 cut(s) 1030
BmuI ACTGGG 1 cut(s) 1030
BpiI GAAGAC 2 cut(s) 68, 500
BpmI CTGGAG 1 cut(s) 360
Bpu10I CCTNAGC 1 cut(s) 116
BsaBI GATNNNNATC 1 cut(s) 14
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 2 cut(s) 1065, 1078
BsaWI WCCGGW 3 cut(s) 125, 143, 209
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 228
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 4 cut(s) 389, 618, 1025, 1084
Bse3DI GCAATG 1 cut(s) 1059
Bse8I GATNNNNATC 1 cut(s) 14
BseBI CCWGG 2 cut(s) 89, 660
BseDI CCNNGG 2 cut(s) 1065, 1078
BseGI GGATG 2 cut(s) 683, 1030
BseJI GATNNNNATC 1 cut(s) 14
BseLI CCNNNNNNNGG 2 cut(s) 167, 228
BseMI GCAATG 1 cut(s) 1059
BseMII CTCAG 2 cut(s) 130, 434
BseNI ACTGG 4 cut(s) 389, 618, 1025, 1084
BseRI GAGGAG 3 cut(s) 19, 948, 951
BseSI GKGCMC 2 cut(s) 151, 802
BseXI GCAGC 3 cut(s) 203, 875, 1033
BsgI GTGCAG 1 cut(s) 333
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 2 cut(s) 943, 1083
BshNI GGYRCC 2 cut(s) 146, 655
BshTI ACCGGT 1 cut(s) 209
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 3 cut(s) 59, 419, 792
BsiSI CCGG 3 cut(s) 126, 144, 210
BslFI GGGAC 1 cut(s) 925
BslI CCNNNNNNNGG 2 cut(s) 167, 228
BsmAI GTCTC 3 cut(s) 31, 70, 182
BsmFI GGGAC 1 cut(s) 925
BsnI GGCC 2 cut(s) 943, 1083
Bso31I GGTCTC 1 cut(s) 182
Bsp1286I GDGCHC 5 cut(s) 59, 151, 419, 792, 802
Bsp143I GATC 3 cut(s) 983, 1000, 1074
Bsp19I CCATGG 1 cut(s) 1078
BspANI GGCC 2 cut(s) 943, 1083
BspCNI CTCAG 2 cut(s) 129, 433
BspLI GGNNCC 3 cut(s) 148, 343, 657
BspPI GGATC 3 cut(s) 991, 995, 1069
BspT107I GGYRCC 2 cut(s) 146, 655
BspTNI GGTCTC 1 cut(s) 182
BsrDI GCAATG 1 cut(s) 1059
BsrFI RCCGGY 1 cut(s) 209
BsrI ACTGG 4 cut(s) 389, 618, 1025, 1084
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 2 cut(s) 1065, 1078
BssMI GATC 3 cut(s) 983, 1000, 1074
BssT1I CCWWGG 1 cut(s) 1078
Bst2UI CCWGG 2 cut(s) 89, 660
Bst4CI ACNGT 1 cut(s) 785
BstC8I GCNNGC 1 cut(s) 925
BstDEI CTNAG 4 cut(s) 116, 420, 812, 917
BstDSI CCRYGG 1 cut(s) 1078
BstF5I GGATG 2 cut(s) 683, 1030
BstHHI GCGC 1 cut(s) 863
BstKTI GATC 3 cut(s) 986, 1003, 1077
BstMAI GTCTC 3 cut(s) 31, 70, 182
BstMBI GATC 3 cut(s) 983, 1000, 1074
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 3 cut(s) 274, 620, 761
BstNI CCWGG 2 cut(s) 89, 660
BstNSI RCATGY 1 cut(s) 927
BstSCI CCNGG 2 cut(s) 87, 658
BstSLI GKGCMC 2 cut(s) 151, 802
BstV1I GCAGC 3 cut(s) 203, 875, 1033
BstV2I GAAGAC 2 cut(s) 68, 500
BstX2I RGATCY 1 cut(s) 1000
BstXI CCANNNNNNTGG 1 cut(s) 396
BstYI RGATCY 1 cut(s) 1000
BsuRI GGCC 2 cut(s) 943, 1083
BtgI CCRYGG 1 cut(s) 1078
BtsCI GGATG 2 cut(s) 683, 1030
BtsI GCAGTG 2 cut(s) 345, 648
BtsIMutI CAGTG 5 cut(s) 290, 345, 625, 648, 790
Cac8I GCNNGC 1 cut(s) 925
CaiI CAGNNNCTG 1 cut(s) 1046
CfoI GCGC 1 cut(s) 863
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 2 cut(s) 386, 941
Csp6I GTAC 1 cut(s) 584
CspAI ACCGGT 1 cut(s) 209
CspCI CAANNNNNGTGG 2 cut(s) 334, 369
CviAII CATG 7 cut(s) 317, 562, 830, 868, 881, 924, 1079
CviQI GTAC 1 cut(s) 584
DdeI CTNAG 4 cut(s) 116, 420, 812, 917
DpnI GATC 3 cut(s) 985, 1002, 1076
DpnII GATC 3 cut(s) 983, 1000, 1074
DraIII CACNNNGTG 1 cut(s) 48
DriI GACNNNNNGTC 1 cut(s) 233
EaeI YGGCCR 1 cut(s) 1081
Eam1105I GACNNNNNGTC 1 cut(s) 233
Ecl136II GAGCTC 1 cut(s) 790
Eco130I CCWWGG 1 cut(s) 1078
Eco24I GRGCYC 1 cut(s) 792
Eco31I GGTCTC 1 cut(s) 182
Eco47I GGWCC 1 cut(s) 386
Eco53kI GAGCTC 1 cut(s) 790
Eco57I CTGAAG 2 cut(s) 464, 564
EcoICRI GAGCTC 1 cut(s) 790
EcoRI GAATTC 2 cut(s) 362, 1029
EcoRII CCWGG 2 cut(s) 87, 658
EcoT14I CCWWGG 1 cut(s) 1078
EcoT38I GRGCYC 1 cut(s) 792
ErhI CCWWGG 1 cut(s) 1078
FaeI CATG 7 cut(s) 320, 565, 833, 871, 884, 927, 1082
FalI AAGNNNNNCTT 2 cut(s) 485, 517
FaqI GGGAC 1 cut(s) 925
FatI CATG 7 cut(s) 316, 561, 829, 867, 880, 923, 1078
Fnu4HI GCNGC 3 cut(s) 217, 864, 1047
FokI GGATG 2 cut(s) 690, 1037
FriOI GRGCYC 1 cut(s) 792
Fsp4HI GCNGC 3 cut(s) 217, 864, 1047
FspBI CTAG 3 cut(s) 62, 287, 803
GlaI GCGC 1 cut(s) 862
GluI GCNGC 3 cut(s) 217, 864, 1047
GsuI CTGGAG 1 cut(s) 360
HaeIII GGCC 2 cut(s) 943, 1083
HapII CCGG 3 cut(s) 126, 144, 210
HhaI GCGC 1 cut(s) 863
Hin1II CATG 7 cut(s) 320, 565, 833, 871, 884, 927, 1082
Hin6I GCGC 1 cut(s) 861
HinP1I GCGC 1 cut(s) 861
HindIII AAGCTT 1 cut(s) 576
HinfI GANTC 5 cut(s) 110, 407, 496, 900, 913
HpaII CCGG 3 cut(s) 126, 144, 210
Hpy166II GTNNAC 5 cut(s) 325, 399, 974, 1018, 1089
Hpy188I TCNGA 3 cut(s) 170, 223, 386
Hpy188III TCNNGA 2 cut(s) 436, 490
Hpy8I GTNNAC 5 cut(s) 325, 399, 974, 1018, 1089
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 3 cut(s) 146, 629, 745
HpyCH4III ACNGT 1 cut(s) 785
HpyCH4IV ACGT 3 cut(s) 44, 591, 796
HpyCH4V TGCA 5 cut(s) 155, 350, 459, 623, 833
HpyF10VI GCNNNNNNNGC 3 cut(s) 274, 620, 761
HpyF3I CTNAG 4 cut(s) 116, 420, 812, 917
HpySE526I ACGT 3 cut(s) 44, 591, 796
Hsp92II CATG 7 cut(s) 320, 565, 833, 871, 884, 927, 1082
HspAI GCGC 1 cut(s) 861
Kzo9I GATC 3 cut(s) 983, 1000, 1074
LmnI GCTCC 5 cut(s) 180, 224, 341, 599, 638
Lsp1109I GCAGC 3 cut(s) 203, 875, 1033
MaeI CTAG 3 cut(s) 62, 287, 803
MaeII ACGT 3 cut(s) 44, 591, 796
MaeIII GTNAC 1 cut(s) 476
MalI GATC 3 cut(s) 985, 1002, 1076
MboI GATC 3 cut(s) 983, 1000, 1074
MboII GAAGA 5 cut(s) 68, 358, 505, 644, 999
MfeI CAATTG 1 cut(s) 672
MflI RGATCY 1 cut(s) 1000
MhlI GDGCHC 5 cut(s) 59, 151, 419, 792, 802
MlsI TGGCCA 1 cut(s) 1083
MluCI AATT 8 cut(s) 130, 362, 535, 603, 672, 949, 958, 1029
MluNI TGGCCA 1 cut(s) 1083
MlyI GAGTC 4 cut(s) 104, 401, 490, 907
MmeI TCCRAC 1 cut(s) 577
Mox20I TGGCCA 1 cut(s) 1083
MroXI GAANNNNTTC 1 cut(s) 755
MscI TGGCCA 1 cut(s) 1083
MseI TTAA 2 cut(s) 12, 1037
MslI CAYNNNNRTG 1 cut(s) 783
Msp20I TGGCCA 1 cut(s) 1083
MspI CCGG 3 cut(s) 126, 144, 210
MspR9I CCNGG 2 cut(s) 89, 660
MunI CAATTG 1 cut(s) 672
MvaI CCWGG 2 cut(s) 89, 660
MwoI GCNNNNNNNGC 3 cut(s) 274, 620, 761
NcoI CCATGG 1 cut(s) 1078
NdeII GATC 3 cut(s) 983, 1000, 1074
NlaIII CATG 7 cut(s) 320, 565, 833, 871, 884, 927, 1082
NlaIV GGNNCC 3 cut(s) 148, 343, 657
NmeAIII GCCGAG 1 cut(s) 1090
NmuCI GTSAC 1 cut(s) 476
NspI RCATGY 1 cut(s) 927
PaeI GCATGC 1 cut(s) 927
PdmI GAANNNNTTC 1 cut(s) 755
PfeI GAWTC 1 cut(s) 900
PinAI ACCGGT 1 cut(s) 209
PkrI GCNGC 3 cut(s) 218, 865, 1048
PleI GAGTC 4 cut(s) 104, 401, 490, 907
PpsI GAGTC 4 cut(s) 104, 401, 490, 907
PshBI ATTAAT 1 cut(s) 12
Psp124BI GAGCTC 1 cut(s) 792
Psp6I CCWGG 2 cut(s) 87, 658
PspGI CCWGG 2 cut(s) 87, 658
PspN4I GGNNCC 3 cut(s) 148, 343, 657
PspPI GGNCC 2 cut(s) 386, 941
PstNI CAGNNNCTG 1 cut(s) 1046
PsuI RGATCY 1 cut(s) 1000
RsaI GTAC 1 cut(s) 585
RsaNI GTAC 1 cut(s) 584
RseI CAYNNNNRTG 1 cut(s) 783
SacI GAGCTC 1 cut(s) 792
SaqAI TTAA 2 cut(s) 12, 1037
SatI GCNGC 3 cut(s) 217, 864, 1047
Sau3AI GATC 3 cut(s) 983, 1000, 1074
Sau96I GGNCC 2 cut(s) 386, 941
SchI GAGTC 4 cut(s) 104, 401, 490, 907
ScrFI CCNGG 2 cut(s) 89, 660
SduI GDGCHC 5 cut(s) 59, 151, 419, 792, 802
SinI GGWCC 1 cut(s) 386
SmiMI CAYNNNNRTG 1 cut(s) 783
SphI GCATGC 1 cut(s) 927
Sse9I AATT 8 cut(s) 130, 362, 535, 603, 672, 949, 958, 1029
SspI AATATT 1 cut(s) 96
SspMI CTAG 3 cut(s) 62, 287, 803
SstI GAGCTC 1 cut(s) 792
StyD4I CCNGG 2 cut(s) 87, 658
StyI CCWWGG 1 cut(s) 1078
TaaI ACNGT 1 cut(s) 785
TaiI ACGT 3 cut(s) 47, 594, 799
TaqI TCGA 1 cut(s) 240
TasI AATT 8 cut(s) 130, 362, 535, 603, 672, 949, 958, 1029
TfiI GAWTC 1 cut(s) 900
Tru1I TTAA 2 cut(s) 12, 1037
Tru9I TTAA 2 cut(s) 12, 1037
TscAI CASTG 5 cut(s) 297, 352, 625, 648, 790
TseFI GTSAC 1 cut(s) 476
TseI GCWGC 3 cut(s) 216, 863, 1046
Tsp45I GTSAC 1 cut(s) 476
TspDTI ATGAA 2 cut(s) 17, 235
TspRI CASTG 5 cut(s) 297, 352, 625, 648, 790
VpaK11BI GGWCC 1 cut(s) 386
VspI ATTAAT 1 cut(s) 12
XapI RAATTY 3 cut(s) 130, 362, 1029
XceI RCATGY 1 cut(s) 927
XmnI GAANNNNTTC 1 cut(s) 755
XspI CTAG 3 cut(s) 62, 287, 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.