RchiOBHm_Chr5g0010211

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0009794
Physical Location & Seq
Forward (+)
6784641 .. 6785428
788 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29100

Sequence Viewer

Length: 546 bp
ATGAAGTTGTTCTTCATCTCGCTAGCCTCTTCATGCTTGAGTTTGTACATAGTTTTACCAAGTGTTCAATCCTACAAAATGATAATTAACTTTGGTGACTCCATTAGCGACACTGGAAATTACCTTCTTACCGGTGCCTTTAAAAATCCGGTCATCGGAAATCTCCCTTATGGAATGACCTTTTTCCATCTTCCAACTGGGCGGAGCTCGGATGGACGTGTTCCTGTTGATTTCCTTACTGAGGCATTTGAGTTGCCATTTTTACCACCCTACCTAACGCTGAAGAAAGGCCAAGATTTTTACCATGGAGTGAACTTTGCTGTTGCTTCACTAGACCCAAAATACTTCTACCAAAGAAAGATTGGATCAACTCTGTTGACAAACTACTCCTTGAGCACTCAGCTTGGTTGGTTCAAGAAGCTCAAGTCCTCACTCTGCAACAATAAGCAAGAATGCGACAACTACTTCAAGAAGGCTCTGCTTCTGGTGGGGGAGATAGGTGGAAACGATTACAATTACCCCTTCTTCGCAGGTGTGACTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

181

Amino Acids

20.3

Weight (kDa)

8.87

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 174 4.2e-10 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 521
Acc36I ACCTGC 1 cut(s) 521
AccB1I GGYRCC 1 cut(s) 134
AciI CCGC 1 cut(s) 202
AclWI GGATC 1 cut(s) 373
AcuI CTGAAG 1 cut(s) 302
AfaI GTAC 1 cut(s) 47
AfiI CCNNNNNNNGG 2 cut(s) 155, 241
AflIII ACRYGT 1 cut(s) 217
AgeI ACCGGT 1 cut(s) 131
AgsI TTSAA 3 cut(s) 68, 415, 469
AjiI CACGTC 1 cut(s) 218
AluBI AGCT 3 cut(s) 207, 403, 421
AluI AGCT 3 cut(s) 207, 403, 421
Alw21I GWGCWC 2 cut(s) 209, 398
AlwI GGATC 1 cut(s) 373
AoxI GGCC 1 cut(s) 289
AsiGI ACCGGT 1 cut(s) 131
Asp700I GAANNNNTTC 1 cut(s) 8
AsuHPI GGTGA 1 cut(s) 107
AsuNHI GCTAGC 1 cut(s) 22
BanI GGYRCC 1 cut(s) 134
BanII GRGCYC 1 cut(s) 209
Bbv12I GWGCWC 2 cut(s) 209, 398
BccI CCATC 2 cut(s) 195, 206
BfaI CTAG 2 cut(s) 23, 332
BfuAI ACCTGC 1 cut(s) 521
BmgBI CACGTC 1 cut(s) 218
BmiI GGNNCC 1 cut(s) 136
BmrI ACTGGG 1 cut(s) 207
BmtI GCTAGC 1 cut(s) 26
BmuI ACTGGG 1 cut(s) 207
BpuEI CTTGAG 3 cut(s) 58, 407, 412
BsaJI CCNNGG 1 cut(s) 304
BsaWI WCCGGW 2 cut(s) 131, 148
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 241
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 2 cut(s) 118, 202
BseDI CCNNGG 1 cut(s) 304
BseGI GGATG 1 cut(s) 217
BseLI CCNNNNNNNGG 2 cut(s) 155, 241
BseMII CTCAG 2 cut(s) 231, 413
BseNI ACTGG 2 cut(s) 118, 202
BshFI GGCC 1 cut(s) 291
BshNI GGYRCC 1 cut(s) 134
BshTI ACCGGT 1 cut(s) 131
BsiHKAI GWGCWC 2 cut(s) 209, 398
BsiSI CCGG 2 cut(s) 132, 149
BslI CCNNNNNNNGG 2 cut(s) 155, 241
BsmI GAATGC 1 cut(s) 458
BsnI GGCC 1 cut(s) 291
Bsp1286I GDGCHC 2 cut(s) 209, 398
Bsp1407I TGTACA 1 cut(s) 45
Bsp143I GATC 1 cut(s) 365
Bsp19I CCATGG 1 cut(s) 304
BspACI CCGC 1 cut(s) 202
BspANI GGCC 1 cut(s) 291
BspCNI CTCAG 2 cut(s) 232, 412
BspLI GGNNCC 1 cut(s) 136
BspMI ACCTGC 1 cut(s) 521
BspOI GCTAGC 1 cut(s) 26
BspPI GGATC 1 cut(s) 373
BspT107I GGYRCC 1 cut(s) 134
BsrFI RCCGGY 1 cut(s) 131
BsrGI TGTACA 1 cut(s) 45
BsrI ACTGG 2 cut(s) 118, 202
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 1 cut(s) 304
BssMI GATC 1 cut(s) 365
BssT1I CCWWGG 1 cut(s) 304
Bst4CI ACNGT 1 cut(s) 541
Bst6I CTCTTC 1 cut(s) 34
BstAUI TGTACA 1 cut(s) 45
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 2 cut(s) 240, 399
BstDSI CCRYGG 1 cut(s) 304
BstENI CCTNNNNNAGG 1 cut(s) 239
BstF5I GGATG 1 cut(s) 217
BstKTI GATC 1 cut(s) 368
BstMBI GATC 1 cut(s) 365
BsuRI GGCC 1 cut(s) 291
BtgI CCRYGG 1 cut(s) 304
BtrI CACGTC 1 cut(s) 218
BtsCI GGATG 1 cut(s) 217
BtsIMutI CAGTG 1 cut(s) 111
BveI ACCTGC 1 cut(s) 521
Cac8I GCNNGC 1 cut(s) 24
Cfr10I RCCGGY 1 cut(s) 131
Csp6I GTAC 1 cut(s) 46
CspAI ACCGGT 1 cut(s) 131
CviAII CATG 2 cut(s) 33, 305
CviJI RGCY 6 cut(s) 26, 207, 291, 403, 421, 476
CviKI_1 RGCY 6 cut(s) 26, 207, 291, 403, 421, 476
CviQI GTAC 1 cut(s) 46
DdeI CTNAG 2 cut(s) 240, 399
DpnI GATC 1 cut(s) 367
DpnII GATC 1 cut(s) 365
DraI TTTAAA 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 34
EarI CTCTTC 1 cut(s) 34
EciI GGCGGA 1 cut(s) 217
Ecl136II GAGCTC 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 304
Eco24I GRGCYC 1 cut(s) 209
Eco53kI GAGCTC 1 cut(s) 207
Eco57I CTGAAG 1 cut(s) 302
EcoICRI GAGCTC 1 cut(s) 207
EcoNI CCTNNNNNAGG 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 304
EcoT38I GRGCYC 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 304
FaeI CATG 2 cut(s) 36, 308
FaiI YATR 4 cut(s) 34, 50, 171, 306
FalI AAGNNNNNCTT 1 cut(s) 28
FatI CATG 2 cut(s) 32, 304
FokI GGATG 1 cut(s) 224
FriOI GRGCYC 1 cut(s) 209
FspBI CTAG 2 cut(s) 23, 332
HaeIII GGCC 1 cut(s) 291
HapII CCGG 2 cut(s) 132, 149
Hin1II CATG 2 cut(s) 36, 308
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HinfI GANTC 1 cut(s) 98
HpaII CCGG 2 cut(s) 132, 149
HphI GGTGA 1 cut(s) 107
Hpy166II GTNNAC 2 cut(s) 313, 378
Hpy188I TCNGA 2 cut(s) 158, 211
Hpy188III TCNNGA 2 cut(s) 415, 469
Hpy8I GTNNAC 2 cut(s) 313, 378
HpyAV CCTTC 3 cut(s) 134, 466, 532
HpyCH4III ACNGT 1 cut(s) 541
HpyCH4IV ACGT 1 cut(s) 217
HpyCH4V TGCA 1 cut(s) 438
HpyF3I CTNAG 2 cut(s) 240, 399
HpySE526I ACGT 1 cut(s) 217
Hsp92II CATG 2 cut(s) 36, 308
Kzo9I GATC 1 cut(s) 365
LmnI GCTCC 1 cut(s) 204
LpnPI CCDG 7 cut(s) 99, 145, 162, 183, 237, 470, 516
MaeI CTAG 2 cut(s) 23, 332
MaeII ACGT 1 cut(s) 217
MaeIII GTNAC 2 cut(s) 95, 535
MalI GATC 1 cut(s) 367
MboI GATC 1 cut(s) 365
MboII GAAGA 5 cut(s) 4, 21, 182, 295, 517
MhlI GDGCHC 2 cut(s) 209, 398
MluCI AATT 3 cut(s) 84, 118, 514
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 1 cut(s) 218
MnlI CCTC 3 cut(s) 37, 235, 439
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 2 cut(s) 87, 141
MspI CCGG 2 cut(s) 132, 149
Mva1269I GAATGC 1 cut(s) 458
NcoI CCATGG 1 cut(s) 304
NdeII GATC 1 cut(s) 365
NheI GCTAGC 1 cut(s) 22
NlaIII CATG 2 cut(s) 36, 308
NlaIV GGNNCC 1 cut(s) 136
NmuCI GTSAC 2 cut(s) 95, 535
PaqCI CACCTGC 1 cut(s) 521
PctI GAATGC 1 cut(s) 458
PdmI GAANNNNTTC 1 cut(s) 8
PinAI ACCGGT 1 cut(s) 131
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
Psp124BI GAGCTC 1 cut(s) 209
PspN4I GGNNCC 1 cut(s) 136
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
SacI GAGCTC 1 cut(s) 209
SaqAI TTAA 2 cut(s) 87, 141
Sau3AI GATC 1 cut(s) 365
SchI GAGTC 1 cut(s) 92
SduI GDGCHC 2 cut(s) 209, 398
SetI ASST 9 cut(s) 126, 182, 209, 220, 276, 405, 423, 502, 535
SmlI CTYRAG 3 cut(s) 37, 391, 422
SmoI CTYRAG 3 cut(s) 37, 391, 422
Sse9I AATT 3 cut(s) 84, 118, 514
SsiI CCGC 1 cut(s) 202
SspMI CTAG 2 cut(s) 23, 332
SstI GAGCTC 1 cut(s) 209
StyI CCWWGG 1 cut(s) 304
TaaI ACNGT 1 cut(s) 541
TaiI ACGT 1 cut(s) 220
TasI AATT 3 cut(s) 84, 118, 514
TatI WGTACW 1 cut(s) 45
Tru1I TTAA 2 cut(s) 87, 141
Tru9I TTAA 2 cut(s) 87, 141
TscAI CASTG 1 cut(s) 118
TseFI GTSAC 2 cut(s) 95, 535
Tsp45I GTSAC 2 cut(s) 95, 535
TspDTI ATGAA 3 cut(s) 4, 17, 21
TspRI CASTG 1 cut(s) 118
XagI CCTNNNNNAGG 1 cut(s) 239
XcmI CCANNNNNNNNNTGG 2 cut(s) 194, 359
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 23, 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.