Rmu_co8154512.1_g000001

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8154512.1
OG0009794
Physical Location & Seq
Reverse (-)
1 .. 606
606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8154512.1_g000001.1.cds

Sequence Viewer

Length: 460 bp
atgaagctgtttatcctgctagcttctttgtgcttgactttgcacgtagtctcatcaagtcttcaacagtatcaagcaatattcaacttcggtgactccctcagtgacaccggaaatttccttctctccgatccccgcgcacccccaaacgtcattggtaagctaccttatggagagaccttttttcaccacgcaaccggccggttctcggatggccgccttattgttgacttcatcgctgaggcatctggattgccgcatttaccaccatatttagccctaaagaaaggccaaaacttttaccaaggagtaaactttgctgttgctggagccactgcacttgaccctgaattcttctaccgaaggaaaatggcatcgattctggccacaaactactccttgagcactcagcttggttggttcaaggagctcaagccctcactctgcaacaccaaacaag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

153

Amino Acids

16.95

Weight (kDa)

8.93

Isoelectric Point (pI)

25.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 138
AciI CCGC 3 cut(s) 136, 217, 257
AclWI GGATC 1 cut(s) 125
AcoI YGGCCR 3 cut(s) 199, 214, 384
AcsI RAATTY 2 cut(s) 115, 350
AfiI CCNNNNNNNGG 1 cut(s) 208
AgsI TTSAA 3 cut(s) 65, 85, 424
AluBI AGCT 5 cut(s) 7, 23, 163, 412, 430
AluI AGCT 5 cut(s) 7, 23, 163, 412, 430
Alw21I GWGCWC 2 cut(s) 407, 432
Alw26I GTCTC 2 cut(s) 55, 170
AlwI GGATC 1 cut(s) 125
AoxI GGCC 4 cut(s) 199, 214, 289, 384
ApoI RAATTY 2 cut(s) 115, 350
AspLEI GCGC 1 cut(s) 140
AsuHPI GGTGA 2 cut(s) 104, 179
AsuNHI GCTAGC 1 cut(s) 19
BalI TGGCCA 1 cut(s) 386
BanII GRGCYC 1 cut(s) 432
BbsI GAAGAC 1 cut(s) 53
Bbv12I GWGCWC 2 cut(s) 407, 432
BbvCI CCTCAGC 1 cut(s) 240
BccI CCATC 1 cut(s) 206
BcoDI GTCTC 2 cut(s) 55, 170
BfaI CTAG 1 cut(s) 20
BisI GCNGC 2 cut(s) 217, 257
BlsI GCNGC 2 cut(s) 218, 258
BmiI GGNNCC 1 cut(s) 331
BmsI GCATC 2 cut(s) 254, 383
BmtI GCTAGC 1 cut(s) 23
BpiI GAAGAC 1 cut(s) 53
BpmI CTGGAG 1 cut(s) 348
Bpu10I CCTNAGC 1 cut(s) 240
BpuEI CTTGAG 2 cut(s) 416, 421
Bsa29I ATCGAT 1 cut(s) 377
BsaAI YACGTR 1 cut(s) 46
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 1 cut(s) 304
BsaWI WCCGGW 1 cut(s) 110
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse118I RCCGGY 2 cut(s) 197, 201
BseCI ATCGAT 1 cut(s) 377
BseDI CCNNGG 1 cut(s) 304
BseGI GGATG 1 cut(s) 217
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMII CTCAG 3 cut(s) 115, 231, 422
BseX3I CGGCCG 1 cut(s) 199
BsgI GTGCAG 1 cut(s) 321
Bsh1236I CGCG 1 cut(s) 138
Bsh1285I CGRYCG 1 cut(s) 202
BshFI GGCC 4 cut(s) 201, 216, 291, 386
BshVI ATCGAT 1 cut(s) 377
BsiEI CGRYCG 1 cut(s) 202
BsiHKAI GWGCWC 2 cut(s) 407, 432
BsiSI CCGG 3 cut(s) 111, 198, 202
BslI CCNNNNNNNGG 1 cut(s) 208
BsmAI GTCTC 2 cut(s) 55, 170
BsnI GGCC 4 cut(s) 201, 216, 291, 386
Bso31I GGTCTC 1 cut(s) 170
Bsp1286I GDGCHC 2 cut(s) 407, 432
Bsp143I GATC 1 cut(s) 130
BspACI CCGC 3 cut(s) 136, 217, 257
BspANI GGCC 4 cut(s) 201, 216, 291, 386
BspCNI CTCAG 3 cut(s) 114, 232, 421
BspDI ATCGAT 1 cut(s) 377
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 1 cut(s) 331
BspOI GCTAGC 1 cut(s) 23
BspPI GGATC 1 cut(s) 125
BspTNI GGTCTC 1 cut(s) 170
BsrFI RCCGGY 2 cut(s) 197, 201
BssAI RCCGGY 2 cut(s) 197, 201
BssECI CCNNGG 1 cut(s) 304
BssMI GATC 1 cut(s) 130
BssT1I CCWWGG 1 cut(s) 304
Bst4CI ACNGT 1 cut(s) 69
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 1 cut(s) 21
BstDEI CTNAG 3 cut(s) 101, 240, 408
BstF5I GGATG 1 cut(s) 217
BstFNI CGCG 1 cut(s) 138
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 1 cut(s) 133
BstMAI GTCTC 2 cut(s) 55, 170
BstMBI GATC 1 cut(s) 130
BstMCI CGRYCG 1 cut(s) 202
BstUI CGCG 1 cut(s) 138
BstV2I GAAGAC 1 cut(s) 53
BstZI CGGCCG 1 cut(s) 199
Bsu15I ATCGAT 1 cut(s) 377
BsuRI GGCC 4 cut(s) 201, 216, 291, 386
BsuTUI ATCGAT 1 cut(s) 377
BtgZI GCGATG 1 cut(s) 220
BtsCI GGATG 1 cut(s) 217
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 2 cut(s) 109, 333
Cac8I GCNNGC 1 cut(s) 21
CfoI GCGC 1 cut(s) 140
Cfr10I RCCGGY 2 cut(s) 197, 201
ClaI ATCGAT 1 cut(s) 377
CspCI CAANNNNNGTGG 2 cut(s) 322, 357
DdeI CTNAG 3 cut(s) 101, 240, 408
DpnI GATC 1 cut(s) 132
DpnII GATC 1 cut(s) 130
EaeI YGGCCR 3 cut(s) 199, 214, 384
EagI CGGCCG 1 cut(s) 199
Ecl136II GAGCTC 1 cut(s) 430
EclXI CGGCCG 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 304
Eco24I GRGCYC 1 cut(s) 432
Eco31I GGTCTC 1 cut(s) 170
Eco52I CGGCCG 1 cut(s) 199
Eco53kI GAGCTC 1 cut(s) 430
EcoICRI GAGCTC 1 cut(s) 430
EcoRI GAATTC 1 cut(s) 350
EcoT14I CCWWGG 1 cut(s) 304
EcoT38I GRGCYC 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 304
FaiI YATR 2 cut(s) 171, 271
FauI CCCGC 1 cut(s) 143
Fnu4HI GCNGC 2 cut(s) 217, 257
FokI GGATG 1 cut(s) 224
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 2 cut(s) 217, 257
FspBI CTAG 1 cut(s) 20
GlaI GCGC 1 cut(s) 139
GluI GCNGC 2 cut(s) 217, 257
GsuI CTGGAG 1 cut(s) 348
HaeIII GGCC 4 cut(s) 201, 216, 291, 386
HapII CCGG 3 cut(s) 111, 198, 202
HhaI GCGC 1 cut(s) 140
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 2 cut(s) 95, 379
HpaII CCGG 3 cut(s) 111, 198, 202
HphI GGTGA 2 cut(s) 104, 179
Hpy166II GTNNAC 2 cut(s) 229, 313
Hpy188I TCNGA 2 cut(s) 130, 211
Hpy188III TCNNGA 1 cut(s) 249
Hpy8I GTNNAC 2 cut(s) 229, 313
HpyAV CCTTC 2 cut(s) 131, 357
HpyCH4III ACNGT 1 cut(s) 69
HpyCH4IV ACGT 2 cut(s) 45, 150
HpyCH4V TGCA 3 cut(s) 43, 338, 447
HpyF3I CTNAG 3 cut(s) 101, 240, 408
HpySE526I ACGT 2 cut(s) 45, 150
HspAI GCGC 1 cut(s) 138
Kzo9I GATC 1 cut(s) 130
LmnI GCTCC 2 cut(s) 329, 427
LpnPI CCDG 8 cut(s) 29, 124, 211, 215, 234, 312, 360, 368
LweI GCATC 2 cut(s) 254, 383
MaeI CTAG 1 cut(s) 20
MaeII ACGT 2 cut(s) 45, 150
MaeIII GTNAC 2 cut(s) 92, 104
MalI GATC 1 cut(s) 132
MboI GATC 1 cut(s) 130
MboII GAAGA 2 cut(s) 53, 346
MhlI GDGCHC 2 cut(s) 407, 432
MlsI TGGCCA 1 cut(s) 386
MluCI AATT 2 cut(s) 115, 350
MluNI TGGCCA 1 cut(s) 386
MlyI GAGTC 1 cut(s) 89
MnlI CCTC 3 cut(s) 110, 235, 448
Mox20I TGGCCA 1 cut(s) 386
MscI TGGCCA 1 cut(s) 386
Msp20I TGGCCA 1 cut(s) 386
MspI CCGG 3 cut(s) 111, 198, 202
MvnI CGCG 1 cut(s) 138
NdeII GATC 1 cut(s) 130
NheI GCTAGC 1 cut(s) 19
NlaIV GGNNCC 1 cut(s) 331
NmuCI GTSAC 2 cut(s) 92, 104
PfeI GAWTC 1 cut(s) 379
PkrI GCNGC 2 cut(s) 218, 258
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Ppu21I YACGTR 1 cut(s) 46
Psp124BI GAGCTC 1 cut(s) 432
PspN4I GGNNCC 1 cut(s) 331
SacI GAGCTC 1 cut(s) 432
SatI GCNGC 2 cut(s) 217, 257
Sau3AI GATC 1 cut(s) 130
SchI GAGTC 1 cut(s) 89
SduI GDGCHC 2 cut(s) 407, 432
SetI ASST 9 cut(s) 9, 25, 48, 153, 165, 169, 182, 414, 432
SfaNI GCATC 2 cut(s) 254, 383
SmlI CTYRAG 2 cut(s) 400, 431
SmoI CTYRAG 2 cut(s) 400, 431
Sse9I AATT 2 cut(s) 115, 350
SsiI CCGC 3 cut(s) 136, 217, 257
SspI AATATT 1 cut(s) 81
SspMI CTAG 1 cut(s) 20
SstI GAGCTC 1 cut(s) 432
StyI CCWWGG 1 cut(s) 304
TaaI ACNGT 1 cut(s) 69
TaiI ACGT 2 cut(s) 48, 153
TaqI TCGA 1 cut(s) 377
TasI AATT 2 cut(s) 115, 350
TauI GCSGC 2 cut(s) 219, 259
TfiI GAWTC 1 cut(s) 379
TscAI CASTG 2 cut(s) 109, 340
TseFI GTSAC 2 cut(s) 92, 104
Tsp45I GTSAC 2 cut(s) 92, 104
TspDTI ATGAA 2 cut(s) 17, 223
TspRI CASTG 2 cut(s) 109, 340
XapI RAATTY 2 cut(s) 115, 350
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.