Rh5CG089000

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0009794
Physical Location & Seq
Forward (+)
6954379 .. 6962506
8128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG089000.1

Sequence Viewer

Length: 597 bp
ATGATAATTAACTTTGGTGACTCCATTAGCGACACTGGAAATTACCTTCTTACCGGTGCCTTTAAAAATCCGGTCATCGGAAATCTCCCTTATGGAATGACCTTTTTCCATCTTCCAACTGGGCGGAGCTCGGATGGACGTGTTCCTGTTGATTTCCTTACTGAGGCATTTGAGTTGCCATTTTTACCACCCTACCTAACGCTGAAGAAAGGCCAAGATTTTTACCATGGAGTGAACTTTGCTGTTGCTTCACTAGACCCAAAATACTTCTACCAAAGAAAGATTGGATCAACTCTGTTGACAAACTACTCCTTGAGCACTCAGCTTGGTTGGTTCAAGAAGCTCAAGTCCTCACTCTGCAACAATAAGCAAGATTGCAATAAACTCCTCTCCAGCTCTCTAGTTCTGGAGGGAAAGATTGGAGGCAACAATTACAATTTACAACGATGTGCTGTTGGCAGGGAAAAATATAGAACAGGTTCAAACATATGTAACATCTCTTCAACCATCCATGATTTATTGGGGGAACAGTCGAAGCATGCTGTGGAGGCGGAGGACCATACCATTACAACTCATCGGCACAATGCGGCTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

198

Amino Acids

22.03

Weight (kDa)

8.83

Isoelectric Point (pI)

35.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 2 - 117 3.1e-07 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 56
AciI CCGC 3 cut(s) 124, 551, 587
AclWI GGATC 1 cut(s) 295
AcuI CTGAAG 1 cut(s) 224
AfiI CCNNNNNNNGG 2 cut(s) 77, 163
AflIII ACRYGT 1 cut(s) 139
AgeI ACCGGT 1 cut(s) 53
AgsI TTSAA 3 cut(s) 337, 483, 504
AjiI CACGTC 1 cut(s) 140
AluBI AGCT 4 cut(s) 129, 325, 343, 396
AluI AGCT 4 cut(s) 129, 325, 343, 396
Alw21I GWGCWC 2 cut(s) 131, 320
AlwI GGATC 1 cut(s) 295
AoxI GGCC 1 cut(s) 211
AsiGI ACCGGT 1 cut(s) 53
Asp700I GAANNNNTTC 1 cut(s) 478
AspS9I GGNCC 1 cut(s) 556
AsuHPI GGTGA 1 cut(s) 29
AvaII GGWCC 1 cut(s) 556
BanI GGYRCC 1 cut(s) 56
BanII GRGCYC 1 cut(s) 131
BarI GAAGNNNNNNTAC 2 cut(s) 484, 516
Bbv12I GWGCWC 2 cut(s) 131, 320
BccI CCATC 3 cut(s) 117, 128, 515
BfaI CTAG 3 cut(s) 254, 401, 595
BisI GCNGC 1 cut(s) 588
BlsI GCNGC 1 cut(s) 589
Bme18I GGWCC 1 cut(s) 556
BmgBI CACGTC 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 556
BmiI GGNNCC 1 cut(s) 58
BmrI ACTGGG 1 cut(s) 129
BmuI ACTGGG 1 cut(s) 129
BpmI CTGGAG 2 cut(s) 376, 428
BpuEI CTTGAG 2 cut(s) 329, 334
BsaJI CCNNGG 1 cut(s) 226
BsaWI WCCGGW 2 cut(s) 53, 70
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 163
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 2 cut(s) 40, 124
BseDI CCNNGG 1 cut(s) 226
BseGI GGATG 2 cut(s) 139, 507
BseLI CCNNNNNNNGG 2 cut(s) 77, 163
BseMII CTCAG 2 cut(s) 153, 335
BseNI ACTGG 2 cut(s) 40, 124
BseRI GAGGAG 1 cut(s) 377
BshFI GGCC 1 cut(s) 213
BshNI GGYRCC 1 cut(s) 56
BshTI ACCGGT 1 cut(s) 53
BsiHKAI GWGCWC 2 cut(s) 131, 320
BsiSI CCGG 2 cut(s) 54, 71
BslI CCNNNNNNNGG 2 cut(s) 77, 163
BsnI GGCC 1 cut(s) 213
Bsp1286I GDGCHC 2 cut(s) 131, 320
Bsp143I GATC 1 cut(s) 287
Bsp19I CCATGG 1 cut(s) 226
BspACI CCGC 3 cut(s) 124, 551, 587
BspANI GGCC 1 cut(s) 213
BspCNI CTCAG 2 cut(s) 154, 334
BspLI GGNNCC 1 cut(s) 58
BspPI GGATC 1 cut(s) 295
BspT107I GGYRCC 1 cut(s) 56
BsrFI RCCGGY 1 cut(s) 53
BsrI ACTGG 2 cut(s) 40, 124
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 226
BssMI GATC 1 cut(s) 287
BssT1I CCWWGG 1 cut(s) 226
Bst4CI ACNGT 1 cut(s) 531
Bst6I CTCTTC 1 cut(s) 505
BstC8I GCNNGC 1 cut(s) 540
BstDEI CTNAG 2 cut(s) 162, 321
BstDSI CCRYGG 1 cut(s) 226
BstENI CCTNNNNNAGG 1 cut(s) 161
BstF5I GGATG 2 cut(s) 139, 507
BstKTI GATC 1 cut(s) 290
BstMBI GATC 1 cut(s) 287
BstMWI GCNNNNNNNGC 1 cut(s) 548
BstNSI RCATGY 1 cut(s) 542
BsuRI GGCC 1 cut(s) 213
BtgI CCRYGG 1 cut(s) 226
BtrI CACGTC 1 cut(s) 140
BtsCI GGATG 2 cut(s) 139, 507
BtsIMutI CAGTG 1 cut(s) 33
Cac8I GCNNGC 1 cut(s) 540
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 1 cut(s) 556
CspAI ACCGGT 1 cut(s) 53
CviAII CATG 3 cut(s) 227, 512, 539
CviJI RGCY 6 cut(s) 129, 213, 325, 343, 396, 590
CviKI_1 RGCY 6 cut(s) 129, 213, 325, 343, 396, 590
DdeI CTNAG 2 cut(s) 162, 321
DpnI GATC 1 cut(s) 289
DpnII GATC 1 cut(s) 287
DraI TTTAAA 1 cut(s) 64
Eam1104I CTCTTC 1 cut(s) 505
EarI CTCTTC 1 cut(s) 505
EciI GGCGGA 2 cut(s) 139, 566
Ecl136II GAGCTC 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 226
Eco24I GRGCYC 1 cut(s) 131
Eco47I GGWCC 1 cut(s) 556
Eco53kI GAGCTC 1 cut(s) 129
Eco57I CTGAAG 1 cut(s) 224
EcoICRI GAGCTC 1 cut(s) 129
EcoNI CCTNNNNNAGG 1 cut(s) 161
EcoT14I CCWWGG 1 cut(s) 226
EcoT38I GRGCYC 1 cut(s) 131
ErhI CCWWGG 1 cut(s) 226
FaeI CATG 3 cut(s) 230, 515, 542
FaiI YATR 8 cut(s) 93, 228, 471, 488, 490, 513, 540, 561
FatI CATG 3 cut(s) 226, 511, 538
FauNDI CATATG 1 cut(s) 488
Fnu4HI GCNGC 1 cut(s) 588
FokI GGATG 2 cut(s) 146, 494
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 1 cut(s) 588
FspBI CTAG 3 cut(s) 254, 401, 595
GluI GCNGC 1 cut(s) 588
GsuI CTGGAG 2 cut(s) 376, 428
HaeIII GGCC 1 cut(s) 213
HapII CCGG 2 cut(s) 54, 71
Hin1II CATG 3 cut(s) 230, 515, 542
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HinfI GANTC 1 cut(s) 20
HpaII CCGG 2 cut(s) 54, 71
HphI GGTGA 1 cut(s) 29
Hpy166II GTNNAC 2 cut(s) 235, 300
Hpy188I TCNGA 2 cut(s) 80, 133
Hpy188III TCNNGA 2 cut(s) 337, 407
Hpy8I GTNNAC 2 cut(s) 235, 300
HpyAV CCTTC 1 cut(s) 56
HpyCH4III ACNGT 1 cut(s) 531
HpyCH4IV ACGT 1 cut(s) 139
HpyCH4V TGCA 2 cut(s) 360, 378
HpyF10VI GCNNNNNNNGC 1 cut(s) 548
HpyF3I CTNAG 2 cut(s) 162, 321
HpySE526I ACGT 1 cut(s) 139
Hsp92II CATG 3 cut(s) 230, 515, 542
Kzo9I GATC 1 cut(s) 287
LmnI GCTCC 1 cut(s) 126
LpnPI CCDG 9 cut(s) 21, 67, 84, 105, 159, 392, 406, 445, 462
MaeI CTAG 3 cut(s) 254, 401, 595
MaeII ACGT 1 cut(s) 139
MaeIII GTNAC 2 cut(s) 17, 491
MalI GATC 1 cut(s) 289
MboI GATC 1 cut(s) 287
MboII GAAGA 3 cut(s) 104, 217, 492
MhlI GDGCHC 2 cut(s) 131, 320
MluCI AATT 4 cut(s) 6, 40, 430, 436
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 1 cut(s) 140
MnlI CCTC 7 cut(s) 157, 361, 398, 403, 416, 541, 547
MroXI GAANNNNTTC 1 cut(s) 478
MseI TTAA 2 cut(s) 9, 63
MspI CCGG 2 cut(s) 54, 71
MwoI GCNNNNNNNGC 1 cut(s) 548
NcoI CCATGG 1 cut(s) 226
NdeI CATATG 1 cut(s) 488
NdeII GATC 1 cut(s) 287
NlaIII CATG 3 cut(s) 230, 515, 542
NlaIV GGNNCC 1 cut(s) 58
NmuCI GTSAC 1 cut(s) 17
NspI RCATGY 1 cut(s) 542
PaeI GCATGC 1 cut(s) 542
PdmI GAANNNNTTC 1 cut(s) 478
PinAI ACCGGT 1 cut(s) 53
PkrI GCNGC 1 cut(s) 589
PleI GAGTC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 14
Psp124BI GAGCTC 1 cut(s) 131
PspN4I GGNNCC 1 cut(s) 58
PspPI GGNCC 1 cut(s) 556
SacI GAGCTC 1 cut(s) 131
SaqAI TTAA 2 cut(s) 9, 63
SatI GCNGC 1 cut(s) 588
Sau3AI GATC 1 cut(s) 287
Sau96I GGNCC 1 cut(s) 556
SchI GAGTC 1 cut(s) 14
SduI GDGCHC 2 cut(s) 131, 320
SetI ASST 9 cut(s) 48, 104, 131, 142, 198, 327, 345, 398, 481
SinI GGWCC 1 cut(s) 556
SmlI CTYRAG 2 cut(s) 313, 344
SmoI CTYRAG 2 cut(s) 313, 344
SphI GCATGC 1 cut(s) 542
Sse9I AATT 4 cut(s) 6, 40, 430, 436
SsiI CCGC 3 cut(s) 124, 551, 587
SspMI CTAG 3 cut(s) 254, 401, 595
SstI GAGCTC 1 cut(s) 131
StyI CCWWGG 1 cut(s) 226
TaaI ACNGT 1 cut(s) 531
TaiI ACGT 1 cut(s) 142
TaqI TCGA 1 cut(s) 533
TasI AATT 4 cut(s) 6, 40, 430, 436
TauI GCSGC 1 cut(s) 590
Tru1I TTAA 2 cut(s) 9, 63
Tru9I TTAA 2 cut(s) 9, 63
TscAI CASTG 1 cut(s) 40
TseFI GTSAC 1 cut(s) 17
Tsp45I GTSAC 1 cut(s) 17
TspRI CASTG 1 cut(s) 40
VpaK11BI GGWCC 1 cut(s) 556
XagI CCTNNNNNAGG 1 cut(s) 161
XceI RCATGY 1 cut(s) 542
XcmI CCANNNNNNNNNTGG 2 cut(s) 116, 281
XmnI GAANNNNTTC 1 cut(s) 478
XspI CTAG 3 cut(s) 254, 401, 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.