FvH4_4g04161

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009794
Physical Location & Seq
Forward (+)
3597672 .. 3599823
2152 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04161.t1

Sequence Viewer

Length: 1101 bp
ATGAAGCTGTTTTTCCTGCTAGCTACTTTGTGCTTCTTCACTTTGGGCAGAGTCTCATCAGGTCTTCCAAAATATGAAGCAATATTCAACTTCGGAGACTCCCTCAGTGACACTGGAAATTTCCTTCTCTCCGATCCCCTCGCATCCCAAGTCATTGCTAAGCTACCTTATGGAGAGACCTTTTTCCACCATGCAACCGGCCGGTCCTCCGACGGCCGCCTTATTGTTGACTTCATCGCTGAGGCATGTGAGTTGCCGCATTTACCACCTTATTTAGCCCTAAAGAAAGGCGGCCAAAACTTTTACCGAGGAGTAAACTTTGCTGTTGCCGGAGCTACTGTACTTGCACCTGAATTCCTTTCCAAGCTGTTGCGAGGTAAAGTGGGAGCAATGCTCACAAACTATTCCTTAAGCGCTCAGCTTGGTTGGTTCAAGGAGCTCAAGCCCTCACTTTGCAACACCAAACAAGAATGTGAGAGCTACTTCAACAAGGCTCTGTTTCTGGTGGGGGAGATCGGTGGAAACGATTACAACTACGGTTTCTTTGCTGGTGGGAGCATTAAACAGCTCAAAGCCTTGGTGCCTCTTGTTGTGGATGCAATCACTATGGCCACCAGTGTATTGATAGAGGAAGGAGCAGTGGAGCTAGTGGTGCCTGGAAATTTGCCAATTGGGTGCTTAGCAGTCTACTTGACTTTTAATGGAAGCCCTAACAAAGCTTATTATGATAAACGAAATGGGTGTTTGAAGGCGTTCAATGCTTTTGCCAAGTACCATAACAAAGAGCTCAAACGTGCCCTAGGAACAGTGGAACAAAAATACCCTCATGCAAGGATCATATATGCCGACTACTATGGTGCTTCCATGCCTTTTTTTCATGCTCCTCAACACTATGGGTTCACAAGTGGGAGTCTAGATGTTTGTTGTGGAGGAGGAGGACCATACAACTTCAACAATTCAGCAAGGTGTGGACAAATTGGTTCCAAGGTCTGCAAAGATCCATCTTCGTATGCAAATTGGGATGGAATTCACTTAACACAAGCAGCTTATAGCCACATTGCCAAGGGTTTGATCCATGGAAAGTTCACCATCCCCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

367

Amino Acids

39.86

Weight (kDa)

8.76

Isoelectric Point (pI)

32.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 356 2.9e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 580, 652
AccI GTMKAC 1 cut(s) 687
AciI CCGC 3 cut(s) 217, 257, 291
AclWI GGATC 4 cut(s) 128, 842, 992, 1066
AcoI YGGCCR 4 cut(s) 199, 214, 292, 609
AcsI RAATTY 4 cut(s) 118, 353, 661, 1026
AfaI GTAC 2 cut(s) 342, 773
AfeI AGCGCT 1 cut(s) 415
AflII CTTAAG 1 cut(s) 409
AgsI TTSAA 6 cut(s) 88, 433, 487, 748, 757, 952
AjnI CCWGG 1 cut(s) 655
Alw21I GWGCWC 2 cut(s) 441, 789
Alw26I GTCTC 3 cut(s) 58, 90, 170
AlwI GGATC 4 cut(s) 128, 842, 992, 1066
Aor51HI AGCGCT 1 cut(s) 415
AoxI GGCC 4 cut(s) 199, 214, 292, 609
ApeKI GCWGC 1 cut(s) 1043
ApoI RAATTY 4 cut(s) 118, 353, 661, 1026
Asp700I GAANNNNTTC 1 cut(s) 752
AspA2I CCTAGG 1 cut(s) 799
AspLEI GCGC 1 cut(s) 416
AspS9I GGNCC 2 cut(s) 204, 938
AsuHPI GGTGA 1 cut(s) 1078
AsuNHI GCTAGC 1 cut(s) 19
AvaII GGWCC 2 cut(s) 204, 938
AvrII CCTAGG 1 cut(s) 799
BaeGI GKGCMC 1 cut(s) 799
BalI TGGCCA 1 cut(s) 611
BanI GGYRCC 2 cut(s) 580, 652
BanII GRGCYC 2 cut(s) 441, 789
BbsI GAAGAC 1 cut(s) 56
Bbv12I GWGCWC 2 cut(s) 441, 789
BbvCI CCTCAGC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 1055
BccI CCATC 3 cut(s) 1009, 1016, 1097
BceAI ACGGC 1 cut(s) 229
BciT130I CCWGG 1 cut(s) 657
BcoDI GTCTC 3 cut(s) 58, 90, 170
BfaI CTAG 5 cut(s) 20, 647, 800, 914, 1099
BfoI RGCGCY 1 cut(s) 417
BfrI CTTAAG 1 cut(s) 409
BisI GCNGC 4 cut(s) 217, 257, 292, 1044
BlnI CCTAGG 1 cut(s) 799
BlpI GCTNAGC 3 cut(s) 159, 417, 679
BlsI GCNGC 4 cut(s) 218, 258, 293, 1045
Bme1390I CCNGG 1 cut(s) 657
Bme18I GGWCC 2 cut(s) 204, 938
BmgT120I GGNCC 2 cut(s) 204, 938
BmiI GGNNCC 3 cut(s) 582, 654, 982
BmrFI CCNGG 1 cut(s) 657
BmsI GCATC 2 cut(s) 152, 586
BmtI GCTAGC 1 cut(s) 23
BpiI GAAGAC 1 cut(s) 56
BplI GAGNNNNNCTC 4 cut(s) 87, 119, 378, 410
Bpu10I CCTNAGC 1 cut(s) 240
Bpu1102I GCTNAGC 3 cut(s) 159, 417, 679
BpuEI CTTGAG 1 cut(s) 425
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 6 cut(s) 307, 576, 799, 984, 1062, 1075
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bse118I RCCGGY 2 cut(s) 197, 201
Bse1I ACTGG 2 cut(s) 118, 615
Bse3DI GCAATG 3 cut(s) 153, 396, 1056
BseBI CCWGG 1 cut(s) 657
BseDI CCNNGG 6 cut(s) 307, 576, 799, 984, 1062, 1075
BseGI GGATG 4 cut(s) 143, 601, 1027, 1089
BseMI GCAATG 3 cut(s) 153, 396, 1056
BseMII CTCAG 3 cut(s) 118, 231, 431
BseNI ACTGG 2 cut(s) 118, 615
BseRI GAGGAG 4 cut(s) 324, 873, 945, 948
BseSI GKGCMC 1 cut(s) 799
BseX3I CGGCCG 2 cut(s) 199, 214
BseXI GCAGC 1 cut(s) 1055
Bsh1285I CGRYCG 2 cut(s) 202, 217
BshFI GGCC 4 cut(s) 201, 216, 294, 611
BshNI GGYRCC 2 cut(s) 580, 652
BsiEI CGRYCG 2 cut(s) 202, 217
BsiHKAI GWGCWC 2 cut(s) 441, 789
BsiSI CCGG 3 cut(s) 198, 202, 330
BsmAI GTCTC 3 cut(s) 58, 90, 170
BsnI GGCC 4 cut(s) 201, 216, 294, 611
Bso31I GGTCTC 1 cut(s) 170
Bsp1286I GDGCHC 3 cut(s) 441, 789, 799
Bsp143I GATC 5 cut(s) 133, 513, 834, 997, 1071
Bsp1720I GCTNAGC 3 cut(s) 159, 417, 679
Bsp19I CCATGG 1 cut(s) 1075
BspACI CCGC 3 cut(s) 217, 257, 291
BspANI GGCC 4 cut(s) 201, 216, 294, 611
BspCNI CTCAG 3 cut(s) 117, 232, 430
BspLI GGNNCC 3 cut(s) 582, 654, 982
BspOI GCTAGC 1 cut(s) 23
BspPI GGATC 4 cut(s) 128, 842, 992, 1066
BspT107I GGYRCC 2 cut(s) 580, 652
BspTI CTTAAG 1 cut(s) 409
BspTNI GGTCTC 1 cut(s) 170
BsrDI GCAATG 3 cut(s) 153, 396, 1056
BsrFI RCCGGY 2 cut(s) 197, 201
BsrI ACTGG 2 cut(s) 118, 615
BssAI RCCGGY 2 cut(s) 197, 201
BssECI CCNNGG 6 cut(s) 307, 576, 799, 984, 1062, 1075
BssMI GATC 5 cut(s) 133, 513, 834, 997, 1071
BssT1I CCWWGG 5 cut(s) 576, 799, 984, 1062, 1075
Bst2UI CCWGG 1 cut(s) 657
Bst4CI ACNGT 3 cut(s) 340, 539, 808
BstAFI CTTAAG 1 cut(s) 409
BstC8I GCNNGC 1 cut(s) 21
BstDEI CTNAG 5 cut(s) 104, 159, 240, 417, 679
BstDSI CCRYGG 1 cut(s) 1075
BstF5I GGATG 4 cut(s) 143, 601, 1027, 1089
BstH2I RGCGCY 1 cut(s) 417
BstHHI GCGC 1 cut(s) 416
BstKTI GATC 5 cut(s) 136, 516, 837, 1000, 1074
BstMAI GTCTC 3 cut(s) 58, 90, 170
BstMBI GATC 5 cut(s) 133, 513, 834, 997, 1071
BstMCI CGRYCG 2 cut(s) 202, 217
BstMWI GCNNNNNNNGC 2 cut(s) 652, 758
BstNI CCWGG 1 cut(s) 657
BstNSI RCATGY 1 cut(s) 249
BstSCI CCNGG 1 cut(s) 655
BstSLI GKGCMC 1 cut(s) 799
BstV1I GCAGC 1 cut(s) 1055
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 1 cut(s) 997
BstYI RGATCY 1 cut(s) 997
BstZI CGGCCG 2 cut(s) 199, 214
BsuRI GGCC 4 cut(s) 201, 216, 294, 611
BtgI CCRYGG 1 cut(s) 1075
BtgZI GCGATG 1 cut(s) 220
BtsCI GGATG 4 cut(s) 143, 601, 1027, 1089
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 5 cut(s) 111, 112, 622, 645, 813
Cac8I GCNNGC 1 cut(s) 21
CfoI GCGC 1 cut(s) 416
Cfr10I RCCGGY 2 cut(s) 197, 201
Cfr13I GGNCC 2 cut(s) 204, 938
Csp6I GTAC 2 cut(s) 341, 772
CviAII CATG 6 cut(s) 191, 246, 827, 865, 878, 1076
CviQI GTAC 2 cut(s) 341, 772
DdeI CTNAG 5 cut(s) 104, 159, 240, 417, 679
DpnI GATC 5 cut(s) 135, 515, 836, 999, 1073
DpnII GATC 5 cut(s) 133, 513, 834, 997, 1071
EaeI YGGCCR 4 cut(s) 199, 214, 292, 609
EagI CGGCCG 2 cut(s) 199, 214
Ecl136II GAGCTC 2 cut(s) 439, 787
EclXI CGGCCG 2 cut(s) 199, 214
Eco130I CCWWGG 5 cut(s) 576, 799, 984, 1062, 1075
Eco24I GRGCYC 2 cut(s) 441, 789
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 2 cut(s) 204, 938
Eco47III AGCGCT 1 cut(s) 415
Eco52I CGGCCG 2 cut(s) 199, 214
Eco53kI GAGCTC 2 cut(s) 439, 787
EcoICRI GAGCTC 2 cut(s) 439, 787
EcoRI GAATTC 2 cut(s) 353, 1026
EcoRII CCWGG 1 cut(s) 655
EcoT14I CCWWGG 5 cut(s) 576, 799, 984, 1062, 1075
EcoT38I GRGCYC 2 cut(s) 441, 789
ErhI CCWWGG 5 cut(s) 576, 799, 984, 1062, 1075
FaeI CATG 6 cut(s) 194, 249, 830, 868, 881, 1079
FatI CATG 6 cut(s) 190, 245, 826, 864, 877, 1075
FblI GTMKAC 1 cut(s) 687
Fnu4HI GCNGC 4 cut(s) 217, 257, 292, 1044
FokI GGATG 4 cut(s) 130, 608, 1034, 1076
FriOI GRGCYC 2 cut(s) 441, 789
Fsp4HI GCNGC 4 cut(s) 217, 257, 292, 1044
FspBI CTAG 5 cut(s) 20, 647, 800, 914, 1099
GlaI GCGC 1 cut(s) 415
GluI GCNGC 4 cut(s) 217, 257, 292, 1044
HaeII RGCGCY 1 cut(s) 417
HaeIII GGCC 4 cut(s) 201, 216, 294, 611
HapII CCGG 3 cut(s) 198, 202, 330
HhaI GCGC 1 cut(s) 416
Hin1II CATG 6 cut(s) 194, 249, 830, 868, 881, 1079
Hin6I GCGC 1 cut(s) 414
HinP1I GCGC 1 cut(s) 414
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HindIII AAGCTT 1 cut(s) 717
HinfI GANTC 3 cut(s) 51, 98, 910
HpaII CCGG 3 cut(s) 198, 202, 330
HphI GGTGA 1 cut(s) 1078
Hpy166II GTNNAC 6 cut(s) 229, 316, 688, 900, 971, 1086
Hpy188I TCNGA 3 cut(s) 95, 133, 211
Hpy188III TCNNGA 1 cut(s) 914
Hpy8I GTNNAC 6 cut(s) 229, 316, 688, 900, 971, 1086
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 3 cut(s) 134, 626, 742
HpyCH4III ACNGT 3 cut(s) 340, 539, 808
HpyCH4IV ACGT 1 cut(s) 793
HpyCH4V TGCA 7 cut(s) 194, 347, 456, 599, 830, 993, 1013
HpyF10VI GCNNNNNNNGC 2 cut(s) 652, 758
HpyF3I CTNAG 5 cut(s) 104, 159, 240, 417, 679
HpySE526I ACGT 1 cut(s) 793
Hsp92II CATG 6 cut(s) 194, 249, 830, 868, 881, 1079
HspAI GCGC 1 cut(s) 414
Kzo9I GATC 5 cut(s) 133, 513, 834, 997, 1071
LmnI GCTCC 7 cut(s) 332, 386, 436, 555, 635, 643, 886
Lsp1109I GCAGC 1 cut(s) 1055
LweI GCATC 2 cut(s) 152, 586
MaeI CTAG 5 cut(s) 20, 647, 800, 914, 1099
MaeII ACGT 1 cut(s) 793
MaeIII GTNAC 1 cut(s) 107
MalI GATC 5 cut(s) 135, 515, 836, 999, 1073
MboI GATC 5 cut(s) 133, 513, 834, 997, 1071
MboII GAAGA 3 cut(s) 28, 56, 996
MfeI CAATTG 1 cut(s) 669
MflI RGATCY 1 cut(s) 997
MhlI GDGCHC 3 cut(s) 441, 789, 799
MlsI TGGCCA 1 cut(s) 611
MluCI AATT 8 cut(s) 118, 353, 661, 669, 955, 975, 1015, 1026
MluNI TGGCCA 1 cut(s) 611
MlyI GAGTC 3 cut(s) 60, 92, 919
MmeI TCCRAC 1 cut(s) 234
Mox20I TGGCCA 1 cut(s) 611
MroXI GAANNNNTTC 1 cut(s) 752
MscI TGGCCA 1 cut(s) 611
MseI TTAA 4 cut(s) 410, 561, 699, 1034
Msp20I TGGCCA 1 cut(s) 611
MspCI CTTAAG 1 cut(s) 409
MspI CCGG 3 cut(s) 198, 202, 330
MspR9I CCNGG 1 cut(s) 657
MunI CAATTG 1 cut(s) 669
MvaI CCWGG 1 cut(s) 657
MwoI GCNNNNNNNGC 2 cut(s) 652, 758
NcoI CCATGG 1 cut(s) 1075
NdeII GATC 5 cut(s) 133, 513, 834, 997, 1071
NheI GCTAGC 1 cut(s) 19
NlaIII CATG 6 cut(s) 194, 249, 830, 868, 881, 1079
NlaIV GGNNCC 3 cut(s) 582, 654, 982
NmuCI GTSAC 1 cut(s) 107
NspI RCATGY 1 cut(s) 249
PcsI WCGNNNNNNNCGW 1 cut(s) 522
PdmI GAANNNNTTC 1 cut(s) 752
PkrI GCNGC 4 cut(s) 218, 258, 293, 1045
PleI GAGTC 3 cut(s) 59, 92, 918
PpsI GAGTC 3 cut(s) 59, 92, 918
Psp124BI GAGCTC 2 cut(s) 441, 789
Psp6I CCWGG 1 cut(s) 655
PspGI CCWGG 1 cut(s) 655
PspN4I GGNNCC 3 cut(s) 582, 654, 982
PspPI GGNCC 2 cut(s) 204, 938
PsuI RGATCY 1 cut(s) 997
RsaI GTAC 2 cut(s) 342, 773
RsaNI GTAC 2 cut(s) 341, 772
SacI GAGCTC 2 cut(s) 441, 789
SaqAI TTAA 4 cut(s) 410, 561, 699, 1034
SatI GCNGC 4 cut(s) 217, 257, 292, 1044
Sau3AI GATC 5 cut(s) 133, 513, 834, 997, 1071
Sau96I GGNCC 2 cut(s) 204, 938
SchI GAGTC 3 cut(s) 60, 92, 919
ScrFI CCNGG 1 cut(s) 657
SduI GDGCHC 3 cut(s) 441, 789, 799
SfaNI GCATC 2 cut(s) 152, 586
SinI GGWCC 2 cut(s) 204, 938
SmlI CTYRAG 2 cut(s) 409, 440
SmoI CTYRAG 2 cut(s) 409, 440
Sse9I AATT 8 cut(s) 118, 353, 661, 669, 955, 975, 1015, 1026
SsiI CCGC 3 cut(s) 217, 257, 291
SspI AATATT 1 cut(s) 84
SspMI CTAG 5 cut(s) 20, 647, 800, 914, 1099
SstI GAGCTC 2 cut(s) 441, 789
StyD4I CCNGG 1 cut(s) 655
StyI CCWWGG 5 cut(s) 576, 799, 984, 1062, 1075
TaaI ACNGT 3 cut(s) 340, 539, 808
TaiI ACGT 1 cut(s) 796
TasI AATT 8 cut(s) 118, 353, 661, 669, 955, 975, 1015, 1026
TatI WGTACW 1 cut(s) 340
TauI GCSGC 3 cut(s) 219, 259, 294
Tru1I TTAA 4 cut(s) 410, 561, 699, 1034
Tru9I TTAA 4 cut(s) 410, 561, 699, 1034
TscAI CASTG 5 cut(s) 112, 118, 622, 645, 813
TseFI GTSAC 1 cut(s) 107
TseI GCWGC 1 cut(s) 1043
Tsp45I GTSAC 1 cut(s) 107
TspDTI ATGAA 4 cut(s) 17, 90, 223, 866
TspRI CASTG 5 cut(s) 112, 118, 622, 645, 813
Vha464I CTTAAG 1 cut(s) 409
VpaK11BI GGWCC 2 cut(s) 204, 938
XapI RAATTY 4 cut(s) 118, 353, 661, 1026
XbaI TCTAGA 1 cut(s) 913
XceI RCATGY 1 cut(s) 249
XmaJI CCTAGG 1 cut(s) 799
XmiI GTMKAC 1 cut(s) 687
XmnI GAANNNNTTC 1 cut(s) 752
XspI CTAG 5 cut(s) 20, 647, 800, 914, 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.