AT5G48800

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007274
Physical Location & Seq
Forward (+)
19786465 .. 19789129
2665 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G48800.1

Sequence Viewer

Length: 1845 bp
ATGGACAAGCATCATCATCAACATCTTTTATTACAGCATCATCAACATCTTCACCATCATCAGAAGCTCTCTCTTGCCAAATCATCCAGACAAAGCTGTAGTGAATGGATCTTTCGAGATGTTCCAAGTGATATAACCATAGAAGTAAACGGTGGAAACTTTGCTCTGCATAAGTTCCCTTTAGTCTCTAGAAGTGGAAGAATCCGAAGAATAGTTGCAGAACATAGAGATTCAGATATCTCCAAAGTGGAGCTTCTTAATCTCCCAGGAGGAGCAGAGACATTCGAACTAGCAGCAAAATTCTGCTATGGGATTAACTTCGAGATCACTTCTTCGAACGTTGCACAGCTCTTTTGTGTCTCTGATTACCTCGAGATGACGGAAGAGTACTCCAAAGATAACTTGGCTTCAAGAACAGAGGAGTATCTTGAGAGCATCGTATGCAAGAATCTTGAGATGTGTGTTCAAGTCTTGAAACAATCCGAGATTCTTCTCCCTCTTGCAGATGAACTGAATATCATAGGTAGATGTATCGATGCCATTGCCTCGAAGGCTTGCGCTGAGCAGATCGCTTCAAGCTTTTCGAGGTTGGAGTATAGTAGCTCAGGGAGGCTTCATATGAGCAGACAAGTGAAAAGCAGTGGTGATGGAGGAGATTGGTGGATCGAAGATCTTTCGGTTCTGAGGATTGATTTGTATCAGAGAGTTATGAATGCAATGAAGTGTCGTGGTGTACGTCCAGAAAGTATAGGAGCTTCTTTGGTTAGTTACGCTGAGAGAGAGCTAACAAAGAGATCTGAACATGAACAAACCATTGTTGAGACAATTGTTACGCTTTTGCCAGTCGAAAACCTCGTGGTTCCGATCAGTTTCCTATTCGGGTTACTGAGAAGAGCAGTGATTCTTGATACTTCAGTTTCCTGCAGGCTTGATCTTGAGAGGAGGTTAGGTTCTCAGCTCGATATGGCGACTCTTGACGATCTCTTGATCCCGTCTTTTCGTCATGCAGGAGACACTTTGTTCGACATTGACACGGTTCACAGAATCTTAGTGAATTTCTCACAGCAAGGAGGAGATGATAGTGAGGATGAAGAGTCTGTGTTTGAATGCGATAGTCCACATTCGCCTTCACAAACCGCCATGTTTAAAGTTGCAAAGCTTGTAGACAGTTATCTTGCTGAGATAGCTCCCGATGCTAACCTTGACCTTTCTAAGTTCTTGCTTATCGCAGAAGCCTTACCGCCTCATGCTCGCACCCTTCACGATGGTTTATACCGCGCCATTGATCTCTATCTTAAGGCACATCAAGGGTTATCAGATTCAGACAAGAAGAAACTATCAAAACTTATTGACTTCCAGAAACTCTCACAAGAAGCTGGAGCACACGCTGCACAAAACGAGCGGCTTCCTCTGCAATCCATAGTTCAAGTTCTCTACTTTGAGCAACTCAAGCTTCGAAGCTCCTTGTGCTCTTCTTACTCAGACGAAGAGCCAAAGCCAAAGCAGCAACAACAGCAGTCATGGAGAATCAATAGCGGAGCTCTCAGTGCAACAATGTCCCCCAAAGACAACTATGCGTCTCTGAGGAGAGAAAACCGAGAACTGAAGCTCGAGTTAGCTAGGTTGAGAATGAGACTCAACGACCTAGAGAAAGAACATATATGTATGAAGAGAGATATGCAGAGGTCTCATTCGCGCAAATTCATGAGCAGTTTCTCAAAGAAAATGGGGAAACTTAGCTTCTTTGGGCATAGCTCTTCAAGAGGATCAAGCTCTCCATCAAAGCAGTCTTTCAGAACAGATTCAAAGGTACTGATGGAGAGAACTTGCGCAAGCACAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

614

Amino Acids

69.55

Weight (kDa)

6.48

Isoelectric Point (pI)

56.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 219 - 459 2.6e-89 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1831
AccBSI CCGCTC 1 cut(s) 1402
AccI GTMKAC 1 cut(s) 1164
AccII CGCG 2 cut(s) 1278, 1696
AciI CCGC 5 cut(s) 1137, 1241, 1276, 1402, 1536
AclI AACGTT 1 cut(s) 339
AclWI GGATC 4 cut(s) 116, 671, 982, 1774
AcsI RAATTY 3 cut(s) 299, 1054, 1700
AcuI CTGAAG 2 cut(s) 897, 1625
AfaI GTAC 3 cut(s) 389, 735, 1812
AflII CTTAAG 1 cut(s) 1295
AgsI TTSAA 8 cut(s) 411, 467, 475, 576, 1106, 1427, 1761, 1806
AjnI CCWGG 1 cut(s) 265
AloI GAACNNNNNNTCC 2 cut(s) 934, 966
Alw21I GWGCWC 3 cut(s) 1384, 1472, 1543
Alw26I GTCTC 8 cut(s) 190, 272, 364, 815, 1005, 1584, 1627, 1692
AlwI GGATC 4 cut(s) 116, 671, 982, 1774
Ama87I CYCGRG 2 cut(s) 371, 1610
ApeKI GCWGC 3 cut(s) 293, 1388, 1504
ApoI RAATTY 3 cut(s) 299, 1054, 1700
ArsI GACNNNNNNTTYG 2 cut(s) 1004, 1036
Asp700I GAANNNNTTC 1 cut(s) 1801
AspLEI GCGC 4 cut(s) 560, 1280, 1698, 1832
AsuHPI GGTGA 2 cut(s) 44, 656
AsuII TTCGAA 3 cut(s) 285, 335, 1456
AvaI CYCGRG 2 cut(s) 371, 1610
BaeI ACNNNNGTAYC 2 cut(s) 900, 933
BanII GRGCYC 1 cut(s) 1543
BarI GAAGNNNNNNTAC 2 cut(s) 739, 771
BauI CACGAG 1 cut(s) 854
Bbv12I GWGCWC 3 cut(s) 1384, 1472, 1543
BbvI GCAGC 3 cut(s) 305, 1375, 1516
BccI CCATC 5 cut(s) 63, 641, 1259, 1786, 1810
BcgI CGANNNNNNTGC 2 cut(s) 524, 558
BciT130I CCWGG 1 cut(s) 267
BcoDI GTCTC 8 cut(s) 190, 272, 364, 815, 1005, 1584, 1627, 1692
BfaI CTAG 4 cut(s) 189, 290, 1620, 1646
BfmI CTRYAG 2 cut(s) 97, 922
BfrI CTTAAG 1 cut(s) 1295
BglI GCCNNNNNGGC 1 cut(s) 551
BglII AGATCT 2 cut(s) 670, 794
BisI GCNGC 4 cut(s) 294, 1389, 1403, 1505
BlpI GCTNAGC 1 cut(s) 561
BlsI GCNGC 4 cut(s) 295, 1390, 1404, 1506
BmcAI AGTACT 1 cut(s) 389
Bme1390I CCNGG 1 cut(s) 267
BmeT110I CYCGRG 2 cut(s) 371, 1610
BmiI GGNNCC 1 cut(s) 861
BmrFI CCNGG 1 cut(s) 267
BmsI GCATC 5 cut(s) 19, 46, 444, 526, 1183
BpmI CTGGAG 1 cut(s) 1398
Bpu10I CCTNAGC 1 cut(s) 604
Bpu1102I GCTNAGC 1 cut(s) 561
Bpu14I TTCGAA 3 cut(s) 285, 335, 1456
BpuEI CTTGAG 4 cut(s) 449, 473, 956, 1433
Bsa29I ATCGAT 1 cut(s) 534
BsaBI GATNNNNATC 2 cut(s) 696, 1290
BsaI GGTCTC 1 cut(s) 1692
BsaJI CCNNGG 1 cut(s) 265
BsaXI ACNNNNNCTCC 2 cut(s) 934, 964
Bse1I ACTGG 1 cut(s) 842
Bse3DI GCAATG 2 cut(s) 540, 723
Bse8I GATNNNNATC 2 cut(s) 696, 1290
BseBI CCWGG 1 cut(s) 267
BseCI ATCGAT 1 cut(s) 534
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 2 cut(s) 83, 1093
BseJI GATNNNNATC 2 cut(s) 696, 1290
BseMI GCAATG 2 cut(s) 540, 723
BseNI ACTGG 1 cut(s) 842
BseRI GAGGAG 6 cut(s) 285, 434, 666, 955, 1086, 1600
BseXI GCAGC 3 cut(s) 305, 1375, 1516
BsgI GTGCAG 1 cut(s) 1374
Bsh1236I CGCG 2 cut(s) 1278, 1696
BshVI ATCGAT 1 cut(s) 534
BsiHKAI GWGCWC 3 cut(s) 1384, 1472, 1543
BsiHKCI CYCGRG 2 cut(s) 371, 1610
BslFI GGGAC 1 cut(s) 1543
BsmAI GTCTC 8 cut(s) 190, 272, 364, 815, 1005, 1584, 1627, 1692
BsmBI CGTCTC 1 cut(s) 1584
BsmFI GGGAC 1 cut(s) 1543
BsmI GAATGC 2 cut(s) 718, 1112
Bso31I GGTCTC 1 cut(s) 1692
BsoBI CYCGRG 2 cut(s) 371, 1610
Bsp119I TTCGAA 3 cut(s) 285, 335, 1456
Bsp1286I GDGCHC 3 cut(s) 1384, 1472, 1543
Bsp1720I GCTNAGC 1 cut(s) 561
BspACI CCGC 5 cut(s) 1137, 1241, 1276, 1402, 1536
BspDI ATCGAT 1 cut(s) 534
BspFNI CGCG 2 cut(s) 1278, 1696
BspHI TCATGA 1 cut(s) 1704
BspLI GGNNCC 1 cut(s) 861
BspMAI CTGCAG 1 cut(s) 926
BspPI GGATC 4 cut(s) 116, 671, 982, 1774
BspQI GCTCTTC 4 cut(s) 886, 1477, 1482, 1762
BspT104I TTCGAA 3 cut(s) 285, 335, 1456
BspTI CTTAAG 1 cut(s) 1295
BspTNI GGTCTC 1 cut(s) 1692
BsrBI CCGCTC 1 cut(s) 1402
BsrDI GCAATG 2 cut(s) 540, 723
BsrI ACTGG 1 cut(s) 842
BssECI CCNNGG 1 cut(s) 265
BssSI CACGAG 1 cut(s) 854
Bst2BI CACGAG 1 cut(s) 854
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 3 cut(s) 152, 1036, 1169
Bst6I CTCTTC 7 cut(s) 378, 886, 1086, 1477, 1482, 1664, 1762
BstAFI CTTAAG 1 cut(s) 1295
BstAPI GCANNNNNTGC 2 cut(s) 441, 1388
BstBI TTCGAA 3 cut(s) 285, 335, 1456
BstC8I GCNNGC 4 cut(s) 556, 926, 1252, 1834
BstF5I GGATG 2 cut(s) 83, 1093
BstFNI CGCG 2 cut(s) 1278, 1696
BstHHI GCGC 4 cut(s) 560, 1280, 1698, 1832
BstMAI GTCTC 8 cut(s) 190, 272, 364, 815, 1005, 1584, 1627, 1692
BstNI CCWGG 1 cut(s) 267
BstSCI CCNGG 1 cut(s) 265
BstSFI CTRYAG 2 cut(s) 97, 922
BstUI CGCG 2 cut(s) 1278, 1696
BstV1I GCAGC 3 cut(s) 305, 1375, 1516
BstX2I RGATCY 3 cut(s) 108, 670, 794
BstYI RGATCY 3 cut(s) 108, 670, 794
Bsu15I ATCGAT 1 cut(s) 534
BsuTUI ATCGAT 1 cut(s) 534
BtsCI GGATG 2 cut(s) 83, 1093
BtsI GCAGTG 2 cut(s) 646, 903
BtsIMutI CAGTG 3 cut(s) 646, 903, 1552
Cac8I GCNNGC 4 cut(s) 556, 926, 1252, 1834
CciI TCATGA 1 cut(s) 1704
CfoI GCGC 4 cut(s) 560, 1280, 1698, 1832
ClaI ATCGAT 1 cut(s) 534
CseI GACGC 1 cut(s) 1566
Csp6I GTAC 3 cut(s) 388, 734, 1811
CviAII CATG 6 cut(s) 803, 1004, 1141, 1247, 1521, 1705
CviQI GTAC 3 cut(s) 388, 734, 1811
DraI TTTAAA 1 cut(s) 1147
Eam1104I CTCTTC 7 cut(s) 378, 886, 1086, 1477, 1482, 1664, 1762
EarI CTCTTC 7 cut(s) 378, 886, 1086, 1477, 1482, 1664, 1762
Ecl136II GAGCTC 1 cut(s) 1541
Eco24I GRGCYC 1 cut(s) 1543
Eco31I GGTCTC 1 cut(s) 1692
Eco32I GATATC 1 cut(s) 238
Eco53kI GAGCTC 1 cut(s) 1541
Eco57I CTGAAG 2 cut(s) 897, 1625
Eco88I CYCGRG 2 cut(s) 371, 1610
EcoICRI GAGCTC 1 cut(s) 1541
EcoRII CCWGG 1 cut(s) 265
EcoRV GATATC 1 cut(s) 238
EcoT38I GRGCYC 1 cut(s) 1543
Esp3I CGTCTC 1 cut(s) 1584
FaeI CATG 6 cut(s) 806, 1007, 1144, 1250, 1524, 1708
FalI AAGNNNNNCTT 2 cut(s) 237, 269
FaqI GGGAC 1 cut(s) 1543
FatI CATG 6 cut(s) 802, 1003, 1140, 1246, 1520, 1704
FauNDI CATATG 1 cut(s) 618
FblI GTMKAC 1 cut(s) 1164
Fnu4HI GCNGC 4 cut(s) 294, 1389, 1403, 1505
FokI GGATG 2 cut(s) 70, 1100
FriOI GRGCYC 1 cut(s) 1543
Fsp4HI GCNGC 4 cut(s) 294, 1389, 1403, 1505
FspBI CTAG 4 cut(s) 189, 290, 1620, 1646
FspI TGCGCA 1 cut(s) 1831
GlaI GCGC 4 cut(s) 559, 1279, 1697, 1831
GluI GCNGC 4 cut(s) 294, 1389, 1403, 1505
GsuI CTGGAG 1 cut(s) 1398
HgaI GACGC 1 cut(s) 1566
HhaI GCGC 4 cut(s) 560, 1280, 1698, 1832
Hin1II CATG 6 cut(s) 806, 1007, 1144, 1250, 1524, 1708
Hin6I GCGC 4 cut(s) 558, 1278, 1696, 1830
HinP1I GCGC 4 cut(s) 558, 1278, 1696, 1830
HindIII AAGCTT 3 cut(s) 577, 1157, 1451
HphI GGTGA 2 cut(s) 44, 656
Hpy166II GTNNAC 5 cut(s) 148, 734, 1039, 1118, 1165
Hpy8I GTNNAC 5 cut(s) 148, 734, 1039, 1118, 1165
HpyAV CCTTC 3 cut(s) 544, 1137, 1268
HpyCH4III ACNGT 3 cut(s) 152, 1036, 1169
HpyCH4IV ACGT 2 cut(s) 339, 736
HpySE526I ACGT 2 cut(s) 339, 736
Hsp92II CATG 6 cut(s) 806, 1007, 1144, 1250, 1524, 1708
HspAI GCGC 4 cut(s) 558, 1278, 1696, 1830
LguI GCTCTTC 4 cut(s) 886, 1477, 1482, 1762
LmnI GCTCC 7 cut(s) 250, 272, 752, 1192, 1379, 1466, 1538
Lsp1109I GCAGC 3 cut(s) 305, 1375, 1516
LweI GCATC 5 cut(s) 19, 46, 444, 526, 1183
MaeI CTAG 4 cut(s) 189, 290, 1620, 1646
MaeII ACGT 2 cut(s) 339, 736
MaeIII GTNAC 3 cut(s) 767, 829, 882
MbiI CCGCTC 1 cut(s) 1402
MfeI CAATTG 1 cut(s) 825
MflI RGATCY 3 cut(s) 108, 670, 794
MhlI GDGCHC 3 cut(s) 1384, 1472, 1543
MluCI AATT 4 cut(s) 299, 825, 1054, 1700
MlyI GAGTC 3 cut(s) 964, 1103, 1629
MmeI TCCRAC 1 cut(s) 570
MroXI GAANNNNTTC 1 cut(s) 1801
MseI TTAA 4 cut(s) 258, 315, 1146, 1296
MspCI CTTAAG 1 cut(s) 1295
MspR9I CCNGG 1 cut(s) 267
MunI CAATTG 1 cut(s) 825
Mva1269I GAATGC 2 cut(s) 718, 1112
MvaI CCWGG 1 cut(s) 267
MvnI CGCG 2 cut(s) 1278, 1696
NdeI CATATG 1 cut(s) 618
NlaIII CATG 6 cut(s) 806, 1007, 1144, 1250, 1524, 1708
NlaIV GGNNCC 1 cut(s) 861
NsbI TGCGCA 1 cut(s) 1831
NspV TTCGAA 3 cut(s) 285, 335, 1456
PaeR7I CTCGAG 2 cut(s) 371, 1610
PagI TCATGA 1 cut(s) 1704
PciSI GCTCTTC 4 cut(s) 886, 1477, 1482, 1762
PcsI WCGNNNNNNNCGW 2 cut(s) 733, 852
PctI GAATGC 2 cut(s) 718, 1112
PdmI GAANNNNTTC 1 cut(s) 1801
PfeI GAWTC 9 cut(s) 201, 230, 448, 487, 901, 1044, 1319, 1527, 1802
PkrI GCNGC 4 cut(s) 295, 1390, 1404, 1506
PleI GAGTC 3 cut(s) 964, 1102, 1629
PpsI GAGTC 3 cut(s) 964, 1102, 1629
Psp124BI GAGCTC 1 cut(s) 1543
Psp1406I AACGTT 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 265
PspN4I GGNNCC 1 cut(s) 861
PspXI VCTCGAGB 1 cut(s) 1610
PstI CTGCAG 1 cut(s) 926
PsuI RGATCY 3 cut(s) 108, 670, 794
RsaI GTAC 3 cut(s) 389, 735, 1812
RsaNI GTAC 3 cut(s) 388, 734, 1811
SacI GAGCTC 1 cut(s) 1543
SapI GCTCTTC 4 cut(s) 886, 1477, 1482, 1762
SaqAI TTAA 4 cut(s) 258, 315, 1146, 1296
SatI GCNGC 4 cut(s) 294, 1389, 1403, 1505
SbfI CCTGCAGG 1 cut(s) 926
ScaI AGTACT 1 cut(s) 389
SchI GAGTC 3 cut(s) 964, 1103, 1629
ScrFI CCNGG 1 cut(s) 267
SdaI CCTGCAGG 1 cut(s) 926
SduI GDGCHC 3 cut(s) 1384, 1472, 1543
SfaNI GCATC 5 cut(s) 19, 46, 444, 526, 1183
SfcI CTRYAG 2 cut(s) 97, 922
Sfr274I CTCGAG 2 cut(s) 371, 1610
SfuI TTCGAA 3 cut(s) 285, 335, 1456
SlaI CTCGAG 2 cut(s) 371, 1610
SmlI CTYRAG 7 cut(s) 371, 428, 452, 935, 1295, 1448, 1610
SmoI CTYRAG 7 cut(s) 371, 428, 452, 935, 1295, 1448, 1610
Sse8387I CCTGCAGG 1 cut(s) 926
Sse9I AATT 4 cut(s) 299, 825, 1054, 1700
SsiI CCGC 5 cut(s) 1137, 1241, 1276, 1402, 1536
SspMI CTAG 4 cut(s) 189, 290, 1620, 1646
SstI GAGCTC 1 cut(s) 1543
StyD4I CCNGG 1 cut(s) 265
TaaI ACNGT 3 cut(s) 152, 1036, 1169
TaiI ACGT 2 cut(s) 342, 739
TasI AATT 4 cut(s) 299, 825, 1054, 1700
TatI WGTACW 1 cut(s) 387
TauI GCSGC 1 cut(s) 1405
TfiI GAWTC 9 cut(s) 201, 230, 448, 487, 901, 1044, 1319, 1527, 1802
Tru1I TTAA 4 cut(s) 258, 315, 1146, 1296
Tru9I TTAA 4 cut(s) 258, 315, 1146, 1296
TscAI CASTG 3 cut(s) 646, 903, 1552
TseI GCWGC 3 cut(s) 293, 1388, 1504
TspDTI ATGAA 8 cut(s) 522, 605, 725, 734, 819, 1104, 1682, 1693
TspGWI ACGGA 1 cut(s) 395
TspRI CASTG 3 cut(s) 646, 903, 1552
Vha464I CTTAAG 1 cut(s) 1295
XapI RAATTY 3 cut(s) 299, 1054, 1700
XbaI TCTAGA 1 cut(s) 188
XcmI CCANNNNNNNNNTGG 1 cut(s) 400
XhoI CTCGAG 2 cut(s) 371, 1610
XmiI GTMKAC 1 cut(s) 1164
XmnI GAANNNNTTC 1 cut(s) 1801
XspI CTAG 4 cut(s) 189, 290, 1620, 1646
ZrmI AGTACT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.