Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007274

Physical Location & Seq

Genomic Coordinates Reverse (-)

30306933 .. 30309772

Gene Structure 2840 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1175400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1866 bp

ATGGACCGCGGCAGTGACAAGCATCAGCATCAGCAGCAGCAGCAACCACAACTATCCGTGGCTAAGAGTTCGCGGCAGCAGCGATACAACGAGTGGATTTTTCGGGACGTTCCAAGCGATATTACAATACAAGTAAATGGAGGGGCTTTCTCACTACATAAGTTCCCTCTGGTCTCTAGAAGTGGGAGAATCCGGAAGTTGGTTGCTGAACACAGGGATTCTGATATCTCAAGAGTGGAGCTTCTTAATCTACCGGGAGGTGCAGAATCATTTGAGTTGGCAGCAAAGTTCTGTTATGGTATTAACTTCGAGATCACATCTTCAAACGTTTCTCAGCTGTGTTGTGCTTCTGATTACCTTGAAATGACTGAAGAGTACTCAAAAGATAATCTTGGTTCGCGCGCCGAAGAATACCTTGAAAGCGTTGTTTGCAAGAACCTTGAAATGTGTGTTGAGGTTTTGCAACAATGCGAGAGTTTACTCCCTCTGGCCGATGAGCTGAGAATAGTTAGCCGTTGCATAGATGCAATAGCCTCAAAGGCTTGTGTAGAGCAAATTGCTTCAAGCTTTTCGCGCTTAGAGTATAGCAGCTCGGGGAGGCTTCACATGAACAGGCAAGCCAAATCTGATGGGGACTGGTGGATAGAAGATCTTTCGGTTCTTCGAGTTGACTTGTACCAAAGAGTCATAACTGCCATGAAGTGCCGTGGAGTCCGCCCTGAGAGTATTGGTGCATCTCTTGTGAACTATGCGCAGAAGGAGTTGACAAAAAAGTCCAGCTTATGGAATATGTCTGGCCAGACAAAAGTTGACGGAATTTCAGCTTCAACTGGGCATGAAAAACTCGTGGTTGAGACAATTGTCAGCCTTCTGCCCGTTGAGAAACTTGTTATTCCAATCAATTTCCTATTCGGGCTTTTGCGAAGCGCTGTAATGCTTGATTGCACAATTGCTTGCAGACTTGATCTGGAGAGGAGAATTGGGTCACAGTTGGATATTGCTACCCTCGATGATCTTCTGATCCCTTCTTTCAAGCACGCTGGTGACACTTTATTCGATGTGGACACAGTGCATAGGATTTTAGTTAACTTTTCTCAGCAAGATGATAGCGAAGAAGATATGGAAGATGCGTCTGTTTTCGAATCTGATAGTCCTCATTCGCCTTCCCAGACCGCTTTGTTTAAAGTTTCAAAGTTGGTGGACAATTACCTTGCTGAAATAGCCCCTGATGCAAACCTCAAGCTTGCTAAGTTCATAGTCATTGCTGATGCTTTACCTGAACACGCACGAACAGTTCATGATGGGCTATATCGAGCCATTGATATTTACTTAAAAGCTCATCAAGGTTTAACCGATACAGACAAGAAGAGGCTCTGCAAAATGATTGATTTCCAAAAGCTCTCACAAGAAGCCGGTGCACACGCTGCACAGAACGAGCGCCTCCCTCTCCAGTCAATAGTACAAGTGCTTTATTTTGAGCAAATAAGGCTCAGAAATTCCTTGGGCTGCTCTAACGAAGATGAACCCAAGCCAATGCACCAGTCATGGCGGATCAGCAGTGGTGCGCTCAGTGCTGCGATGTCTCCACGGGACAACTATGCATCATTGAGGAGAGAAAACCGTGAGCTAAAGCTTGAGCTAGCTCGGTTAAGAATGAGACTAAATGATCTTGAGAGAGAACATGTATGCATGAAGAGGGATATGCAAAGGTCCAACTCTCGTAAATTTATGAGTTCTTTCTCCAAGAAGATTGGCAAGCTAAGCTTCTTTGCAAATAGTTCTTCAAGGGCATCCACTTCTCCGTCAAGGCATTCTTATAGAACTGATTCTAAAGTGATCGAGAGAACGTGTGCAAGCACAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BTB NPH3

Protein Analysis

622

Amino Acids

70.11

Weight (kDa)

6.49

Isoelectric Point (pI)

45.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BTB	PF00651	38 - 123	2.5e-06	BTB/POZ domain
NPH3	PF03000	212 - 471	3.1e-95	NPH3 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007274)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G48800
fragaria_vesca	FvH4_2g19060 FvH4_2g19060 FvH4_2g19060 FvH4_2g19060
malus_domestica	MD05G1175400.v1.1 MD10G1164500.v1.1
prunus_persica	Prupe.8G203100_v2.0.a1
pyrus_communis	pycom05g16220 pycom10g14180
rosa_chinensis	RchiOBHm_Chr6g0284881
rosa_laevigata	RLG00000012705
rosa_multiflora	Rmu_co8481861.1_g000001 Rmu_sc0000686.1_g000019
rosa_roxburghii	Rroxscaffold_7G00184270
rosa_rugosa	Rorug06G0167700 Rorug06G0167800 Rorug06G0167900 Rorug06G0167900 Rorug06G0168000
rosa_samantha	Rh6AG280300 Rh6BG280600 Rh6CG282600 Rh6DG275700
rosa_wichuraiana	Rw6G024090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	772
Acc16I	TGCGCA	1 cut(s)	753
AccB7I	CCANNNNNTGG	1 cut(s)	783
AccII	CGCG	5 cut(s)	9, 73, 400, 402, 574
AccIII	TCCGGA	1 cut(s)	192
AciI	CCGC	6 cut(s)	7, 9, 73, 715, 1173, 1549
AclI	AACGTT	1 cut(s)	327
AclWI	GGATC	2 cut(s)	1015, 1559
AcoI	YGGCCR	2 cut(s)	489, 796
AcsI	RAATTY	3 cut(s)	816, 1495, 1724
AcuI	CTGAAG	1 cut(s)	390
AfaI	GTAC	3 cut(s)	377, 677, 1461
AfeI	AGCGCT	1 cut(s)	928
AfiI	CCNNNNNNNGG	2 cut(s)	199, 783
AflIII	ACRYGT	2 cut(s)	1681, 1847
AgsI	TTSAA	9 cut(s)	324, 362, 419, 443, 564, 828, 1033, 1191, 1785
AleI	CACNNNNGTG	1 cut(s)	1041
Alw21I	GWGCWC	1 cut(s)	1420
Alw26I	GTCTC	4 cut(s)	178, 848, 1587, 1651
Alw44I	GTGCAC	1 cut(s)	1416
AlwI	GGATC	2 cut(s)	1015, 1559
Ama87I	CYCGRG	1 cut(s)	592
Aor13HI	TCCGGA	1 cut(s)	192
Aor51HI	AGCGCT	1 cut(s)	928
AoxI	GGCC	2 cut(s)	489, 796
ApaLI	GTGCAC	1 cut(s)	1416
ApoI	RAATTY	3 cut(s)	816, 1495, 1724
ArsI	GACNNNNNNTTYG	2 cut(s)	1037, 1069
Asp700I	GAANNNNTTC	1 cut(s)	1825
AspLEI	GCGC	7 cut(s)	402, 404, 576, 754, 929, 1440, 1567
AspS9I	GGNCC	2 cut(s)	4, 1710
AsuC2I	CCSGG	1 cut(s)	255
AsuHPI	GGTGA	1 cut(s)	1055
AsuII	TTCGAA	1 cut(s)	1140
AsuNHI	GCTAGC	1 cut(s)	1639
AvaI	CYCGRG	1 cut(s)	592
AvaII	GGWCC	2 cut(s)	4, 1710
BaeGI	GKGCMC	1 cut(s)	1420
BalI	TGGCCA	1 cut(s)	798
BauI	CACGAG	1 cut(s)	845
Bbv12I	GWGCWC	1 cut(s)	1420
BccI	CCATC	2 cut(s)	623, 1295
BceAI	ACGGC	2 cut(s)	498, 690
BcnI	CCSGG	1 cut(s)	255
BcoDI	GTCTC	4 cut(s)	178, 848, 1587, 1651
BfaI	CTAG	2 cut(s)	177, 1640
BfoI	RGCGCY	2 cut(s)	930, 1441
BglI	GCCNNNNNGGC	1 cut(s)	539
BglII	AGATCT	1 cut(s)	649
BlpI	GCTNAGC	1 cut(s)	1760
BmcAI	AGTACT	1 cut(s)	377
Bme1390I	CCNGG	1 cut(s)	255
Bme18I	GGWCC	2 cut(s)	4, 1710
BmeT110I	CYCGRG	1 cut(s)	592
BmgT120I	GGNCC	2 cut(s)	4, 1710
BmrFI	CCNGG	1 cut(s)	255
BmrI	ACTGGG	1 cut(s)	840
BmsI	GCATC	9 cut(s)	31, 37, 514, 743, 1117, 1219, 1258, 1610, 1799
BmtI	GCTAGC	1 cut(s)	1643
BmuI	ACTGGG	1 cut(s)	840
BoxI	GACNNNNGTC	1 cut(s)	860
BpmI	CTGGAG	2 cut(s)	989, 1433
Bpu1102I	GCTNAGC	1 cut(s)	1760
Bpu14I	TTCGAA	1 cut(s)	1140
BpuEI	CTTGAG	4 cut(s)	214, 1223, 1655, 1691
BpuMI	CCSGG	1 cut(s)	255
BsaI	GGTCTC	1 cut(s)	178
BsaJI	CCNNGG	5 cut(s)	7, 57, 706, 1500, 1586
BsaWI	WCCGGW	1 cut(s)	192
Bsc4I	CCNNNNNNNGG	2 cut(s)	199, 783
Bse118I	RCCGGY	1 cut(s)	1412
Bse1I	ACTGG	4 cut(s)	641, 835, 1450, 1540
Bse3DI	GCAATG	1 cut(s)	1260
BseAI	TCCGGA	1 cut(s)	192
BseDI	CCNNGG	5 cut(s)	7, 57, 706, 1500, 1586
BseGI	GGATG	1 cut(s)	1790
BseLI	CCNNNNNNNGG	2 cut(s)	199, 783
BseMI	GCAATG	1 cut(s)	1260
BseMII	CTCAG	6 cut(s)	347, 491, 711, 1109, 1504, 1582
BseNI	ACTGG	4 cut(s)	641, 835, 1450, 1540
BsePI	GCGCGC	1 cut(s)	400
BseRI	GAGGAG	2 cut(s)	988, 1624
BseSI	GKGCMC	1 cut(s)	1420
BsgI	GTGCAG	2 cut(s)	282, 1410
Bsh1236I	CGCG	5 cut(s)	9, 73, 400, 402, 574
BshFI	GGCC	2 cut(s)	491, 798
BsiHKAI	GWGCWC	1 cut(s)	1420
BsiHKCI	CYCGRG	1 cut(s)	592
BsiSI	CCGG	3 cut(s)	193, 254, 1413
BslFI	GGGAC	3 cut(s)	119, 647, 1604
BslI	CCNNNNNNNGG	2 cut(s)	199, 783
BsmAI	GTCTC	4 cut(s)	178, 848, 1587, 1651
BsmFI	GGGAC	3 cut(s)	119, 647, 1604
BsmI	GAATGC	1 cut(s)	1810
BsnI	GGCC	2 cut(s)	491, 798
Bso31I	GGTCTC	1 cut(s)	178
BsoBI	CYCGRG	1 cut(s)	592
Bsp119I	TTCGAA	1 cut(s)	1140
Bsp1286I	GDGCHC	1 cut(s)	1420
Bsp13I	TCCGGA	1 cut(s)	192
Bsp143I	GATC	8 cut(s)	312, 649, 964, 1012, 1020, 1551, 1666, 1836
Bsp1720I	GCTNAGC	1 cut(s)	1760
BspACI	CCGC	6 cut(s)	7, 9, 73, 715, 1173, 1549
BspANI	GGCC	2 cut(s)	491, 798
BspCNI	CTCAG	6 cut(s)	346, 492, 712, 1108, 1503, 1581
BspEI	TCCGGA	1 cut(s)	192
BspFNI	CGCG	5 cut(s)	9, 73, 400, 402, 574
BspHI	TCATGA	1 cut(s)	1297
BspOI	GCTAGC	1 cut(s)	1643
BspPI	GGATC	2 cut(s)	1015, 1559
BspT104I	TTCGAA	1 cut(s)	1140
BspTNI	GGTCTC	1 cut(s)	178
BsrDI	GCAATG	1 cut(s)	1260
BsrFI	RCCGGY	1 cut(s)	1412
BsrI	ACTGG	4 cut(s)	641, 835, 1450, 1540
BssAI	RCCGGY	1 cut(s)	1412
BssECI	CCNNGG	5 cut(s)	7, 57, 706, 1500, 1586
BssHII	GCGCGC	1 cut(s)	400
BssMI	GATC	8 cut(s)	312, 649, 964, 1012, 1020, 1551, 1666, 1836
BssSI	CACGAG	1 cut(s)	845
BssT1I	CCWWGG	1 cut(s)	1500
Bst2BI	CACGAG	1 cut(s)	845
Bst4CI	ACNGT	4 cut(s)	990, 1069, 1294, 1622
Bst6I	CTCTTC	3 cut(s)	366, 1361, 1688
BstAPI	GCANNNNNTGC	1 cut(s)	1424
BstBI	TTCGAA	1 cut(s)	1140
BstC8I	GCNNGC	8 cut(s)	402, 618, 955, 1038, 1245, 1641, 1757, 1855
BstDSI	CCRYGG	4 cut(s)	7, 57, 706, 1586
BstF5I	GGATG	1 cut(s)	1790
BstFNI	CGCG	5 cut(s)	9, 73, 400, 402, 574
BstH2I	RGCGCY	2 cut(s)	930, 1441
BstHHI	GCGC	7 cut(s)	402, 404, 576, 754, 929, 1440, 1567
BstKTI	GATC	8 cut(s)	315, 652, 967, 1015, 1023, 1554, 1669, 1839
BstMAI	GTCTC	4 cut(s)	178, 848, 1587, 1651
BstMBI	GATC	8 cut(s)	312, 649, 964, 1012, 1020, 1551, 1666, 1836
BstNSI	RCATGY	1 cut(s)	1685
BstPAI	GACNNNNGTC	1 cut(s)	860
BstSCI	CCNGG	1 cut(s)	253
BstSLI	GKGCMC	1 cut(s)	1420
BstUI	CGCG	5 cut(s)	9, 73, 400, 402, 574
BstX2I	RGATCY	1 cut(s)	649
BstYI	RGATCY	1 cut(s)	649
BsuRI	GGCC	2 cut(s)	491, 798
BtgI	CCRYGG	4 cut(s)	7, 57, 706, 1586
BtgZI	GCGATG	1 cut(s)	1592
BtsCI	GGATG	1 cut(s)	1790
BtsI	GCAGTG	2 cut(s)	19, 1564
BtsIMutI	CAGTG	4 cut(s)	19, 1074, 1564, 1576
Cac8I	GCNNGC	8 cut(s)	402, 618, 955, 1038, 1245, 1641, 1757, 1855
CciI	TCATGA	1 cut(s)	1297
CfoI	GCGC	7 cut(s)	402, 404, 576, 754, 929, 1440, 1567
Cfr10I	RCCGGY	1 cut(s)	1412
Cfr13I	GGNCC	2 cut(s)	4, 1710
Cfr42I	CCGCGG	1 cut(s)	10
CseI	GACGC	1 cut(s)	1119
Csp6I	GTAC	3 cut(s)	376, 676, 1460
CspCI	CAANNNNNGTGG	2 cut(s)	1179, 1214
CviAII	CATG	7 cut(s)	607, 697, 836, 1298, 1545, 1682, 1690
CviQI	GTAC	3 cut(s)	376, 676, 1460
DpnI	GATC	8 cut(s)	314, 651, 966, 1014, 1022, 1553, 1668, 1838
DpnII	GATC	8 cut(s)	312, 649, 964, 1012, 1020, 1551, 1666, 1836
DraI	TTTAAA	1 cut(s)	1183
DrdI	GACNNNNNNGTC	1 cut(s)	772
DseDI	GACNNNNNNGTC	1 cut(s)	772
EaeI	YGGCCR	2 cut(s)	489, 796
Eam1104I	CTCTTC	3 cut(s)	366, 1361, 1688
EarI	CTCTTC	3 cut(s)	366, 1361, 1688
EciI	GGCGGA	2 cut(s)	704, 1564
Eco130I	CCWWGG	1 cut(s)	1500
Eco31I	GGTCTC	1 cut(s)	178
Eco32I	GATATC	1 cut(s)	226
Eco47I	GGWCC	2 cut(s)	4, 1710
Eco47III	AGCGCT	1 cut(s)	928
Eco57I	CTGAAG	1 cut(s)	390
Eco88I	CYCGRG	1 cut(s)	592
EcoRV	GATATC	1 cut(s)	226
EcoT14I	CCWWGG	1 cut(s)	1500
EcoT22I	ATGCAT	2 cut(s)	1603, 1691
ErhI	CCWWGG	1 cut(s)	1500
FaeI	CATG	7 cut(s)	610, 700, 839, 1301, 1548, 1685, 1693
FaqI	GGGAC	3 cut(s)	119, 647, 1604
FatI	CATG	7 cut(s)	606, 696, 835, 1297, 1544, 1681, 1689
FokI	GGATG	1 cut(s)	1777
FspBI	CTAG	2 cut(s)	177, 1640
FspI	TGCGCA	1 cut(s)	753
GlaI	GCGC	7 cut(s)	401, 403, 575, 753, 928, 1439, 1566
GsuI	CTGGAG	2 cut(s)	989, 1433
HaeII	RGCGCY	2 cut(s)	930, 1441
HaeIII	GGCC	2 cut(s)	491, 798
HapII	CCGG	3 cut(s)	193, 254, 1413
HgaI	GACGC	1 cut(s)	1119
HhaI	GCGC	7 cut(s)	402, 404, 576, 754, 929, 1440, 1567
Hin1II	CATG	7 cut(s)	610, 700, 839, 1301, 1548, 1685, 1693
Hin6I	GCGC	7 cut(s)	400, 402, 574, 752, 927, 1438, 1565
HinP1I	GCGC	7 cut(s)	400, 402, 574, 752, 927, 1438, 1565
HincII	GTYRAC	4 cut(s)	670, 765, 811, 1087
HindII	GTYRAC	4 cut(s)	670, 765, 811, 1087
HindIII	AAGCTT	4 cut(s)	565, 1241, 1631, 1762
HinfI	GANTC	7 cut(s)	189, 218, 266, 684, 711, 1142, 1826
HpaI	GTTAAC	1 cut(s)	1087
HpaII	CCGG	3 cut(s)	193, 254, 1413
HphI	GGTGA	1 cut(s)	1055
Hpy166II	GTNNAC	9 cut(s)	479, 670, 745, 765, 811, 1063, 1087, 1201, 1418
Hpy188I	TCNGA	6 cut(s)	223, 352, 628, 1020, 1147, 1493
Hpy188III	TCNNGA	9 cut(s)	104, 177, 193, 231, 310, 968, 1298, 1670, 1840
Hpy8I	GTNNAC	9 cut(s)	479, 670, 745, 765, 811, 1063, 1087, 1201, 1418
HpyAV	CCTTC	4 cut(s)	751, 878, 1035, 1173
HpyCH4III	ACNGT	4 cut(s)	990, 1069, 1294, 1622
HpyCH4IV	ACGT	3 cut(s)	108, 327, 1847
HpySE526I	ACGT	3 cut(s)	108, 327, 1847
Hsp92II	CATG	7 cut(s)	610, 700, 839, 1301, 1548, 1685, 1693
HspAI	GCGC	7 cut(s)	400, 402, 574, 752, 927, 1438, 1565
Kpn2I	TCCGGA	1 cut(s)	192
KspAI	GTTAAC	1 cut(s)	1087
KspI	CCGCGG	1 cut(s)	10
Kzo9I	GATC	8 cut(s)	312, 649, 964, 1012, 1020, 1551, 1666, 1836
LmnI	GCTCC	1 cut(s)	238
LweI	GCATC	9 cut(s)	31, 37, 514, 743, 1117, 1219, 1258, 1610, 1799
MaeI	CTAG	2 cut(s)	177, 1640
MaeII	ACGT	3 cut(s)	108, 327, 1847
MaeIII	GTNAC	3 cut(s)	14, 984, 1043
MalI	GATC	8 cut(s)	314, 651, 966, 1014, 1022, 1553, 1668, 1838
MboI	GATC	8 cut(s)	312, 649, 964, 1012, 1020, 1551, 1666, 1836
MfeI	CAATTG	2 cut(s)	858, 948
MflI	RGATCY	1 cut(s)	649
MhlI	GDGCHC	1 cut(s)	1420
MlsI	TGGCCA	1 cut(s)	798
MluCI	AATT	9 cut(s)	555, 816, 858, 901, 948, 978, 1204, 1495, 1724
MluNI	TGGCCA	1 cut(s)	798
MlyI	GAGTC	2 cut(s)	693, 720
MmeI	TCCRAC	2 cut(s)	972, 1737
Mox20I	TGGCCA	1 cut(s)	798
Mph1103I	ATGCAT	2 cut(s)	1603, 1691
MroI	TCCGGA	1 cut(s)	192
MroXI	GAANNNNTTC	1 cut(s)	1825
MscI	TGGCCA	1 cut(s)	798
MseI	TTAA	7 cut(s)	246, 303, 1086, 1182, 1331, 1349, 1649
MslI	CAYNNNNRTG	1 cut(s)	1041
Msp20I	TGGCCA	1 cut(s)	798
MspA1I	CMGCKG	2 cut(s)	9, 337
MspI	CCGG	3 cut(s)	193, 254, 1413
MspR9I	CCNGG	1 cut(s)	255
MunI	CAATTG	2 cut(s)	858, 948
Mva1269I	GAATGC	1 cut(s)	1810
MvnI	CGCG	5 cut(s)	9, 73, 400, 402, 574
NciI	CCSGG	1 cut(s)	255
NdeII	GATC	8 cut(s)	312, 649, 964, 1012, 1020, 1551, 1666, 1836
NheI	GCTAGC	1 cut(s)	1639
NlaIII	CATG	7 cut(s)	610, 700, 839, 1301, 1548, 1685, 1693
NmuCI	GTSAC	3 cut(s)	14, 984, 1043
NsbI	TGCGCA	1 cut(s)	753
NsiI	ATGCAT	2 cut(s)	1603, 1691
NspI	RCATGY	1 cut(s)	1685
NspV	TTCGAA	1 cut(s)	1140
OliI	CACNNNNGTG	1 cut(s)	1041
PagI	TCATGA	1 cut(s)	1297
PauI	GCGCGC	1 cut(s)	400
PciI	ACATGT	1 cut(s)	1681
PcsI	WCGNNNNNNNCGW	1 cut(s)	114
PctI	GAATGC	1 cut(s)	1810
PdmI	GAANNNNTTC	1 cut(s)	1825
PfeI	GAWTC	5 cut(s)	189, 218, 266, 1142, 1826
PflMI	CCANNNNNTGG	1 cut(s)	783
PleI	GAGTC	2 cut(s)	692, 719
PpsI	GAGTC	2 cut(s)	692, 719
PscI	ACATGT	1 cut(s)	1681
PshAI	GACNNNNGTC	1 cut(s)	860
Psp1406I	AACGTT	1 cut(s)	327
PspPI	GGNCC	2 cut(s)	4, 1710
PsuI	RGATCY	1 cut(s)	649
PteI	GCGCGC	1 cut(s)	400
PvuII	CAGCTG	1 cut(s)	337
RsaI	GTAC	3 cut(s)	377, 677, 1461
RsaNI	GTAC	3 cut(s)	376, 676, 1460
RseI	CAYNNNNRTG	1 cut(s)	1041
SacII	CCGCGG	1 cut(s)	10
SaqAI	TTAA	7 cut(s)	246, 303, 1086, 1182, 1331, 1349, 1649
Sau3AI	GATC	8 cut(s)	312, 649, 964, 1012, 1020, 1551, 1666, 1836
Sau96I	GGNCC	2 cut(s)	4, 1710
ScaI	AGTACT	1 cut(s)	377
SchI	GAGTC	2 cut(s)	693, 720
ScrFI	CCNGG	1 cut(s)	255
SduI	GDGCHC	1 cut(s)	1420
SfaNI	GCATC	9 cut(s)	31, 37, 514, 743, 1117, 1219, 1258, 1610, 1799
Sfr303I	CCGCGG	1 cut(s)	10
SfuI	TTCGAA	1 cut(s)	1140
SgrBI	CCGCGG	1 cut(s)	10
SinI	GGWCC	2 cut(s)	4, 1710
SmiMI	CAYNNNNRTG	1 cut(s)	1041
SmlI	CTYRAG	4 cut(s)	229, 1238, 1634, 1670
SmoI	CTYRAG	4 cut(s)	229, 1238, 1634, 1670
Sse9I	AATT	9 cut(s)	555, 816, 858, 901, 948, 978, 1204, 1495, 1724
SsiI	CCGC	6 cut(s)	7, 9, 73, 715, 1173, 1549
SspMI	CTAG	2 cut(s)	177, 1640
StyD4I	CCNGG	1 cut(s)	253
StyI	CCWWGG	1 cut(s)	1500
TaaI	ACNGT	4 cut(s)	990, 1069, 1294, 1622
TaiI	ACGT	3 cut(s)	111, 330, 1850
TaqI	TCGA	7 cut(s)	309, 664, 1008, 1056, 1140, 1312, 1839
TasI	AATT	9 cut(s)	555, 816, 858, 901, 948, 978, 1204, 1495, 1724
TatI	WGTACW	2 cut(s)	375, 1459
TauI	GCSGC	2 cut(s)	12, 76
TfiI	GAWTC	5 cut(s)	189, 218, 266, 1142, 1826
Tru1I	TTAA	7 cut(s)	246, 303, 1086, 1182, 1331, 1349, 1649
Tru9I	TTAA	7 cut(s)	246, 303, 1086, 1182, 1331, 1349, 1649
TscAI	CASTG	4 cut(s)	19, 1074, 1564, 1576
TseFI	GTSAC	3 cut(s)	14, 984, 1043
Tsp45I	GTSAC	3 cut(s)	14, 984, 1043
TspDTI	ATGAA	7 cut(s)	623, 713, 852, 1243, 1286, 1536, 1706
TspGWI	ACGGA	3 cut(s)	46, 828, 1791
TspRI	CASTG	4 cut(s)	19, 1074, 1564, 1576
Van91I	CCANNNNNTGG	1 cut(s)	783
VneI	GTGCAC	1 cut(s)	1416
VpaK11BI	GGWCC	2 cut(s)	4, 1710
XapI	RAATTY	3 cut(s)	816, 1495, 1724
XbaI	TCTAGA	1 cut(s)	176
XceI	RCATGY	1 cut(s)	1685
XmnI	GAANNNNTTC	1 cut(s)	1825
XspI	CTAG	2 cut(s)	177, 1640
ZrmI	AGTACT	1 cut(s)	377
Zsp2I	ATGCAT	2 cut(s)	1603, 1691

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.