pycom05g16220

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007274
Physical Location & Seq
Reverse (-)
19559204 .. 19560167
964 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g16220.1

Sequence Viewer

Length: 858 bp
ATGATCTTCTCGATCCCTTCTTTCAAGCACGCTGGTGACACTTTATTTGATGTCGATACAGTGCATAGGATTTTAGTTAACTTTTCTCAGCAAGATGATAGCGAAGAAGATATGGAAGATGCGTCTGTTTTTGAATCTGATAGTCCTCATTCACCTTCCCAGACCGCTTTGTTTAAAGTTTCAAAGTTGGTGGACAATTACCTTGCTGAAATAGCTCCTGATGCAAACCTCAAGCTTGCTAAGTTCATAGTCATCGCTGATGCTTTACCTGAACACGCACGAACAGTTCATGATGGGCTATATCGAGCCATTGATATTTACTTAAAAGCTCATCAAGGTTTAACAGATACAGACAAGAAGAGGCTCTGCAAAATGATTGATTTCCAAAAGCTCTCACAAGAAGCAGGTGCACATGCTGCACAGAACGAGCGCCTCCCTCTCCAGTCAATAGTACAAGTGCTTTATTTTGAGCAAATAAGGCTCAGAAATTCCTTGGGCTGCTCTAATGAAGATGAACCCAAGCCAATGCACCAGTCATGGCGGATCAGCAGTGGTGCGCTCAGTGCAGCGATGTCTCCACGAGACAACTATGCATCATTGAGGAGAGAAAATCGTGAGCTAAAGCTTGAGCTAGCTCGGTTAAGAATGAGACTAAATGATCTTGAGAGAGAACATGTATGCATGAAGAGGGATATGCAAAGGTCCAACTCTCGTAAATTTATGAGTTCTTTCTCCAAGAAGATTGGCAAGCTAAGCTTCTTTGCAAATAGTTCTTCAAGGGCATCCACTTCTCCGTCAAGGCATTCTTATAGAACTGATTCTAAAGTGATTGAGAGAACGTGTGCAAGCACAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

286

Amino Acids

32.44

Weight (kDa)

8.28

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 5 - 135 6.8e-49 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 395
Acc36I ACCTGC 1 cut(s) 395
AciI CCGC 2 cut(s) 165, 541
AclWI GGATC 2 cut(s) 7, 551
AcsI RAATTY 2 cut(s) 487, 716
AfaI GTAC 1 cut(s) 453
AflIII ACRYGT 2 cut(s) 673, 839
AgsI TTSAA 4 cut(s) 25, 134, 183, 777
AleI CACNNNNGTG 1 cut(s) 33
Alw21I GWGCWC 1 cut(s) 412
Alw26I GTCTC 3 cut(s) 576, 579, 643
Alw44I GTGCAC 1 cut(s) 408
AlwI GGATC 2 cut(s) 7, 551
ApaLI GTGCAC 1 cut(s) 408
ApeKI GCWGC 3 cut(s) 416, 498, 566
ApoI RAATTY 2 cut(s) 487, 716
ArsI GACNNNNNNTTYG 2 cut(s) 29, 61
Asp700I GAANNNNTTC 1 cut(s) 817
AspLEI GCGC 2 cut(s) 432, 559
AspS9I GGNCC 1 cut(s) 702
AsuHPI GGTGA 2 cut(s) 47, 144
AsuNHI GCTAGC 1 cut(s) 631
AvaII GGWCC 1 cut(s) 702
BaeGI GKGCMC 1 cut(s) 412
BauI CACGAG 1 cut(s) 579
Bbv12I GWGCWC 1 cut(s) 412
BbvI GCAGC 3 cut(s) 403, 485, 578
BccI CCATC 1 cut(s) 287
BcoDI GTCTC 3 cut(s) 576, 579, 643
BfaI CTAG 1 cut(s) 632
BfoI RGCGCY 1 cut(s) 433
BfuAI ACCTGC 1 cut(s) 395
BisI GCNGC 3 cut(s) 417, 499, 567
BlpI GCTNAGC 1 cut(s) 752
BlsI GCNGC 3 cut(s) 418, 500, 568
Bme18I GGWCC 1 cut(s) 702
BmgT120I GGNCC 1 cut(s) 702
BmsI GCATC 5 cut(s) 109, 211, 250, 602, 791
BmtI GCTAGC 1 cut(s) 635
BpmI CTGGAG 1 cut(s) 425
Bpu1102I GCTNAGC 1 cut(s) 752
BpuEI CTTGAG 3 cut(s) 215, 647, 683
BsaJI CCNNGG 1 cut(s) 492
Bse1I ACTGG 2 cut(s) 442, 532
BseDI CCNNGG 1 cut(s) 492
BseGI GGATG 1 cut(s) 782
BseMII CTCAG 3 cut(s) 101, 496, 574
BseNI ACTGG 2 cut(s) 442, 532
BseRI GAGGAG 1 cut(s) 616
BseSI GKGCMC 1 cut(s) 412
BseXI GCAGC 3 cut(s) 403, 485, 578
BsgI GTGCAG 2 cut(s) 402, 585
BsiHKAI GWGCWC 1 cut(s) 412
BsmAI GTCTC 3 cut(s) 576, 579, 643
BsmI GAATGC 1 cut(s) 802
Bsp1286I GDGCHC 1 cut(s) 412
Bsp143I GATC 4 cut(s) 3, 12, 543, 658
Bsp1720I GCTNAGC 1 cut(s) 752
BspACI CCGC 2 cut(s) 165, 541
BspCNI CTCAG 3 cut(s) 100, 495, 573
BspHI TCATGA 1 cut(s) 289
BspMI ACCTGC 1 cut(s) 395
BspOI GCTAGC 1 cut(s) 635
BspPI GGATC 2 cut(s) 7, 551
BsrI ACTGG 2 cut(s) 442, 532
BssECI CCNNGG 1 cut(s) 492
BssMI GATC 4 cut(s) 3, 12, 543, 658
BssSI CACGAG 1 cut(s) 579
BssT1I CCWWGG 1 cut(s) 492
Bst2BI CACGAG 1 cut(s) 579
Bst4CI ACNGT 2 cut(s) 61, 286
Bst6I CTCTTC 2 cut(s) 353, 680
BstAPI GCANNNNNTGC 1 cut(s) 416
BstC8I GCNNGC 5 cut(s) 30, 237, 633, 749, 847
BstDEI CTNAG 5 cut(s) 87, 240, 482, 560, 752
BstF5I GGATG 1 cut(s) 782
BstH2I RGCGCY 1 cut(s) 433
BstHHI GCGC 2 cut(s) 432, 559
BstKTI GATC 4 cut(s) 6, 15, 546, 661
BstMAI GTCTC 3 cut(s) 576, 579, 643
BstMBI GATC 4 cut(s) 3, 12, 543, 658
BstMWI GCNNNNNNNGC 6 cut(s) 212, 221, 416, 478, 563, 753
BstNSI RCATGY 2 cut(s) 416, 677
BstSLI GKGCMC 1 cut(s) 412
BstV1I GCAGC 3 cut(s) 403, 485, 578
BtgZI GCGATG 2 cut(s) 238, 584
BtsCI GGATG 1 cut(s) 782
BtsI GCAGTG 1 cut(s) 556
BtsIMutI CAGTG 3 cut(s) 66, 556, 568
BveI ACCTGC 1 cut(s) 395
Cac8I GCNNGC 5 cut(s) 30, 237, 633, 749, 847
CciI TCATGA 1 cut(s) 289
CfoI GCGC 2 cut(s) 432, 559
Cfr13I GGNCC 1 cut(s) 702
CseI GACGC 1 cut(s) 111
Csp6I GTAC 1 cut(s) 452
CspCI CAANNNNNGTGG 2 cut(s) 171, 206
CviAII CATG 5 cut(s) 290, 413, 537, 674, 682
CviQI GTAC 1 cut(s) 452
DdeI CTNAG 5 cut(s) 87, 240, 482, 560, 752
DpnI GATC 4 cut(s) 5, 14, 545, 660
DpnII GATC 4 cut(s) 3, 12, 543, 658
DraI TTTAAA 1 cut(s) 175
Eam1104I CTCTTC 2 cut(s) 353, 680
EarI CTCTTC 2 cut(s) 353, 680
EciI GGCGGA 1 cut(s) 556
Eco130I CCWWGG 1 cut(s) 492
Eco47I GGWCC 1 cut(s) 702
EcoT14I CCWWGG 1 cut(s) 492
EcoT22I ATGCAT 2 cut(s) 595, 683
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 5 cut(s) 293, 416, 540, 677, 685
FalI AAGNNNNNCTT 4 cut(s) 740, 772, 790, 822
FatI CATG 5 cut(s) 289, 412, 536, 673, 681
Fnu4HI GCNGC 3 cut(s) 417, 499, 567
FokI GGATG 1 cut(s) 769
Fsp4HI GCNGC 3 cut(s) 417, 499, 567
FspBI CTAG 1 cut(s) 632
GlaI GCGC 2 cut(s) 431, 558
GluI GCNGC 3 cut(s) 417, 499, 567
GsuI CTGGAG 1 cut(s) 425
HaeII RGCGCY 1 cut(s) 433
HgaI GACGC 1 cut(s) 111
HhaI GCGC 2 cut(s) 432, 559
Hin1II CATG 5 cut(s) 293, 416, 540, 677, 685
Hin6I GCGC 2 cut(s) 430, 557
HinP1I GCGC 2 cut(s) 430, 557
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HindIII AAGCTT 3 cut(s) 233, 623, 754
HinfI GANTC 2 cut(s) 134, 818
HpaI GTTAAC 1 cut(s) 79
HphI GGTGA 2 cut(s) 47, 144
Hpy166II GTNNAC 3 cut(s) 79, 193, 410
Hpy188I TCNGA 2 cut(s) 139, 485
Hpy188III TCNNGA 5 cut(s) 10, 218, 290, 614, 662
Hpy8I GTNNAC 3 cut(s) 79, 193, 410
HpyAV CCTTC 2 cut(s) 27, 165
HpyCH4III ACNGT 2 cut(s) 61, 286
HpyCH4IV ACGT 1 cut(s) 839
HpyF10VI GCNNNNNNNGC 6 cut(s) 212, 221, 416, 478, 563, 753
HpyF3I CTNAG 5 cut(s) 87, 240, 482, 560, 752
HpySE526I ACGT 1 cut(s) 839
Hsp92II CATG 5 cut(s) 293, 416, 540, 677, 685
HspAI GCGC 2 cut(s) 430, 557
KspAI GTTAAC 1 cut(s) 79
Kzo9I GATC 4 cut(s) 3, 12, 543, 658
LmnI GCTCC 1 cut(s) 220
LpnPI CCDG 7 cut(s) 18, 173, 231, 282, 390, 455, 545
Lsp1109I GCAGC 3 cut(s) 403, 485, 578
LweI GCATC 5 cut(s) 109, 211, 250, 602, 791
MaeI CTAG 1 cut(s) 632
MaeII ACGT 1 cut(s) 839
MaeIII GTNAC 1 cut(s) 35
MalI GATC 4 cut(s) 5, 14, 545, 660
MboI GATC 4 cut(s) 3, 12, 543, 658
MboII GAAGA 8 cut(s) 116, 119, 128, 370, 521, 697, 751, 765
MhlI GDGCHC 1 cut(s) 412
MluCI AATT 3 cut(s) 196, 487, 716
MmeI TCCRAC 1 cut(s) 729
MnlI CCTC 7 cut(s) 156, 239, 354, 443, 447, 594, 681
Mph1103I ATGCAT 2 cut(s) 595, 683
MroXI GAANNNNTTC 1 cut(s) 817
MseI TTAA 5 cut(s) 78, 174, 323, 341, 641
MslI CAYNNNNRTG 1 cut(s) 33
Mva1269I GAATGC 1 cut(s) 802
MwoI GCNNNNNNNGC 6 cut(s) 212, 221, 416, 478, 563, 753
NdeII GATC 4 cut(s) 3, 12, 543, 658
NheI GCTAGC 1 cut(s) 631
NlaIII CATG 5 cut(s) 293, 416, 540, 677, 685
NmuCI GTSAC 1 cut(s) 35
NsiI ATGCAT 2 cut(s) 595, 683
NspI RCATGY 2 cut(s) 416, 677
OliI CACNNNNGTG 1 cut(s) 33
PagI TCATGA 1 cut(s) 289
PaqCI CACCTGC 1 cut(s) 395
PciI ACATGT 1 cut(s) 673
PctI GAATGC 1 cut(s) 802
PdmI GAANNNNTTC 1 cut(s) 817
PfeI GAWTC 2 cut(s) 134, 818
PkrI GCNGC 3 cut(s) 418, 500, 568
PscI ACATGT 1 cut(s) 673
PspPI GGNCC 1 cut(s) 702
RsaI GTAC 1 cut(s) 453
RsaNI GTAC 1 cut(s) 452
RseI CAYNNNNRTG 1 cut(s) 33
SaqAI TTAA 5 cut(s) 78, 174, 323, 341, 641
SatI GCNGC 3 cut(s) 417, 499, 567
Sau3AI GATC 4 cut(s) 3, 12, 543, 658
Sau96I GGNCC 1 cut(s) 702
SduI GDGCHC 1 cut(s) 412
SfaNI GCATC 5 cut(s) 109, 211, 250, 602, 791
SinI GGWCC 1 cut(s) 702
SmiMI CAYNNNNRTG 1 cut(s) 33
SmlI CTYRAG 3 cut(s) 230, 626, 662
SmoI CTYRAG 3 cut(s) 230, 626, 662
Sse9I AATT 3 cut(s) 196, 487, 716
SsiI CCGC 2 cut(s) 165, 541
SspMI CTAG 1 cut(s) 632
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 2 cut(s) 61, 286
TaiI ACGT 1 cut(s) 842
TaqI TCGA 3 cut(s) 11, 54, 304
TasI AATT 3 cut(s) 196, 487, 716
TatI WGTACW 1 cut(s) 451
TfiI GAWTC 2 cut(s) 134, 818
Tru1I TTAA 5 cut(s) 78, 174, 323, 341, 641
Tru9I TTAA 5 cut(s) 78, 174, 323, 341, 641
TscAI CASTG 3 cut(s) 66, 556, 568
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 3 cut(s) 416, 498, 566
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 5 cut(s) 235, 278, 522, 528, 698
TspGWI ACGGA 1 cut(s) 783
TspRI CASTG 3 cut(s) 66, 556, 568
VneI GTGCAC 1 cut(s) 408
VpaK11BI GGWCC 1 cut(s) 702
XapI RAATTY 2 cut(s) 487, 716
XceI RCATGY 2 cut(s) 416, 677
XmnI GAANNNNTTC 1 cut(s) 817
XspI CTAG 1 cut(s) 632
Zsp2I ATGCAT 2 cut(s) 595, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.