AT5G50360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011674
Physical Location & Seq
Reverse (-)
20505089 .. 20506354
1266 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50360.1

Sequence Viewer

Length: 876 bp
ATGAATTTCCGGGGAGGTTGTTGTATTGCGAGGTACGGTGGTAGTGGTGGTGGAAATGATATGTCGAAGGTTGATCGGATTATGCTCCGGTACCGTCCGATTGCACCGAGACCTGATTCTGGTGGATCTCCTGCTTCACCGACGGAGAAGAATGGATCGGTGATAACTAACGTTTCGTCGAGATCACGGAGAGGTAAGAGAAAATATTCAAAGGAGAATAATAGTAGCAGTACCGGATCCGTTAATAGTAACGGTAACAGCAAACGACAGAGAAACGATGAGACGAAGAACGGATCTGGTGGTGGTAGAGAGATCGTGACGTTGCCTCTTCTTCCAGAGACTCCTGAGAAGAAAGACTCGCCGTTGAAGGCTAAGGCGGCGCCGGAGTTAGGTGCGGCGGCGTTGTGGCTGAGTTTTAATGACGGAGCTAGTTATAACAGACGTTATCAGACAGAGTTAATGACGGAAACGGTTGTGTCGTCGTTGTTGACGGTGGAGTGTGTAACGGAGAGATTGATGGAGGGAGAGTATGAGTTAGGGTGTACGGACGAGGAGAGGAAGATGAATCTTGAGAGAGACACGTGTCCTGGTTTTATATCGGACGGTTTAGGGAGAGTCATTTGGACGAACGGTTCTTACAGAGAGTTGGTGGTTGGGAAAGATCATGAGCAGTGTAGTAAGATGAGTGTGTGGCTTGTGATGAAGGAGAAGCCTTTGGTAACGTACCGGACTTTTACGTGTAGAATGAGATTGCAGTACACGTGTCGTGATAAGGAGGTGAGTTCGATTACTTCGTTCTGTGATGTTTGGAGGATGAGTGACGGTGGCTTTGCGTGGCGGCTTGATGTTGATGCTGCACTTTGCCTTGGACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

291

Amino Acids

32.3

Weight (kDa)

8.96

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7950 PF25821 159 - 288 1.6e-49 Domain of unknown function (DUF7950)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011674)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48510 AT5G50360 AT5G63350
fragaria_vesca FvH4_5g07700
malus_domestica MD06G1147800.v1.1 MD14G1162100.v1.1
prunus_persica Prupe.5G154700_v2.0.a1
pyrus_communis pycom14g13490 pycom72g00090
rosa_chinensis RchiOBHm_Chr7g0190771
rosa_laevigata RLG00000004500
rosa_multiflora Rmu_co8372469.1_g000001
rosa_roxburghii Rroxscaffold_3G00264340
rosa_rugosa Rorug06G0506700
rosa_samantha Rh7AG113400 Rh7BG115900 Rh7CG118400 Rh7DG117400
rosa_wichuraiana Rw7G009900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 435
Acc65I GGTACC 1 cut(s) 90
AccB1I GGYRCC 2 cut(s) 90, 379
AciI CCGC 4 cut(s) 377, 395, 398, 838
AclI AACGTT 1 cut(s) 171
AclWI GGATC 5 cut(s) 133, 163, 231, 244, 301
AcsI RAATTY 1 cut(s) 4
AcvI CACGTG 2 cut(s) 582, 762
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 6 cut(s) 35, 92, 232, 544, 725, 758
AfiI CCNNNNNNNGG 2 cut(s) 119, 389
AflIII ACRYGT 5 cut(s) 579, 581, 737, 759, 761
AgsI TTSAA 2 cut(s) 210, 367
AjnI CCWGG 1 cut(s) 586
AloI GAACNNNNNNTCC 2 cut(s) 616, 648
AluBI AGCT 1 cut(s) 428
AluI AGCT 1 cut(s) 428
Alw26I GTCTC 4 cut(s) 103, 275, 332, 570
AlwI GGATC 5 cut(s) 133, 163, 231, 244, 301
ApeKI GCWGC 1 cut(s) 854
ApoI RAATTY 1 cut(s) 4
ArsI GACNNNNNNTTYG 2 cut(s) 812, 844
Asp700I GAANNNNTTC 1 cut(s) 205
Asp718I GGTACC 1 cut(s) 90
AspLEI GCGC 1 cut(s) 382
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 3 cut(s) 129, 172, 790
BaeI ACNNNNGTAYC 2 cut(s) 25, 58
BamHI GGATCC 1 cut(s) 236
BanI GGYRCC 2 cut(s) 90, 379
BbrPI CACGTG 2 cut(s) 582, 762
BbvI GCAGC 1 cut(s) 841
BccI CCATC 1 cut(s) 511
BceAI ACGGC 1 cut(s) 346
BciT130I CCWGG 1 cut(s) 588
BcnI CCSGG 1 cut(s) 11
BcoDI GTCTC 4 cut(s) 103, 275, 332, 570
BfaI CTAG 1 cut(s) 429
BfoI RGCGCY 1 cut(s) 383
BisI GCNGC 5 cut(s) 378, 396, 399, 839, 855
BlsI GCNGC 5 cut(s) 379, 397, 400, 840, 856
Bme1390I CCNGG 2 cut(s) 11, 588
BmiI GGNNCC 3 cut(s) 92, 238, 381
BmrFI CCNGG 2 cut(s) 11, 588
BmsI GCATC 1 cut(s) 841
BoxI GACNNNNGTC 1 cut(s) 582
Bpu10I CCTNAGC 1 cut(s) 372
BpuEI CTTGAG 1 cut(s) 590
BpuMI CCSGG 1 cut(s) 11
BsaAI YACGTR 3 cut(s) 582, 738, 762
BsaHI GRCGYC 1 cut(s) 380
BsaI GGTCTC 1 cut(s) 103
BsaJI CCNNGG 2 cut(s) 10, 865
BsaWI WCCGGW 3 cut(s) 87, 233, 726
BsaXI ACNNNNNCTCC 4 cut(s) 512, 542, 802, 832
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 389
BseBI CCWGG 1 cut(s) 588
BseDI CCNNGG 2 cut(s) 10, 865
BseGI GGATG 1 cut(s) 819
BseLI CCNNNNNNNGG 2 cut(s) 119, 389
BseMII CTCAG 2 cut(s) 336, 401
BseRI GAGGAG 1 cut(s) 566
BseXI GCAGC 1 cut(s) 841
BsgI GTGCAG 1 cut(s) 840
BshNI GGYRCC 2 cut(s) 90, 379
BsiSI CCGG 5 cut(s) 10, 88, 234, 383, 727
BslI CCNNNNNNNGG 2 cut(s) 119, 389
BsmAI GTCTC 4 cut(s) 103, 275, 332, 570
BsmBI CGTCTC 1 cut(s) 275
Bso31I GGTCTC 1 cut(s) 103
Bsp143I GATC 8 cut(s) 73, 125, 155, 182, 236, 293, 312, 661
BspACI CCGC 4 cut(s) 377, 395, 398, 838
BspCNI CTCAG 2 cut(s) 337, 402
BspHI TCATGA 1 cut(s) 664
BspLI GGNNCC 3 cut(s) 92, 238, 381
BspPI GGATC 5 cut(s) 133, 163, 231, 244, 301
BspT107I GGYRCC 2 cut(s) 90, 379
BspTNI GGTCTC 1 cut(s) 103
BssECI CCNNGG 2 cut(s) 10, 865
BssMI GATC 8 cut(s) 73, 125, 155, 182, 236, 293, 312, 661
BssNI GRCGYC 1 cut(s) 380
BssT1I CCWWGG 1 cut(s) 865
Bst2UI CCWGG 1 cut(s) 588
Bst4CI ACNGT 9 cut(s) 38, 95, 254, 472, 493, 605, 632, 824, 873
Bst6I CTCTTC 1 cut(s) 333
BstACI GRCGYC 1 cut(s) 380
BstBAI YACGTR 3 cut(s) 582, 738, 762
BstDEI CTNAG 3 cut(s) 345, 372, 410
BstF5I GGATG 1 cut(s) 819
BstH2I RGCGCY 1 cut(s) 383
BstHHI GCGC 1 cut(s) 382
BstKTI GATC 8 cut(s) 76, 128, 158, 185, 239, 296, 315, 664
BstMAI GTCTC 4 cut(s) 103, 275, 332, 570
BstMBI GATC 8 cut(s) 73, 125, 155, 182, 236, 293, 312, 661
BstMWI GCNNNNNNNGC 1 cut(s) 377
BstNI CCWGG 1 cut(s) 588
BstPAI GACNNNNGTC 1 cut(s) 582
BstSCI CCNGG 2 cut(s) 9, 586
BstV1I GCAGC 1 cut(s) 841
BstX2I RGATCY 3 cut(s) 125, 236, 293
BstYI RGATCY 3 cut(s) 125, 236, 293
BtsCI GGATG 1 cut(s) 819
BtsI GCAGTG 1 cut(s) 677
BtsIMutI CAGTG 1 cut(s) 677
CciI TCATGA 1 cut(s) 664
CfoI GCGC 1 cut(s) 382
Csp6I GTAC 6 cut(s) 34, 91, 231, 543, 724, 757
CviAII CATG 1 cut(s) 665
CviJI RGCY 7 cut(s) 371, 409, 428, 694, 712, 828, 841
CviKI_1 RGCY 7 cut(s) 371, 409, 428, 694, 712, 828, 841
CviQI GTAC 6 cut(s) 34, 91, 231, 543, 724, 757
DdeI CTNAG 3 cut(s) 345, 372, 410
DinI GGCGCC 1 cut(s) 381
DpnI GATC 8 cut(s) 75, 127, 157, 184, 238, 295, 314, 663
DpnII GATC 8 cut(s) 73, 125, 155, 182, 236, 293, 312, 661
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
Eco130I CCWWGG 1 cut(s) 865
Eco31I GGTCTC 1 cut(s) 103
Eco72I CACGTG 2 cut(s) 582, 762
EcoRII CCWGG 1 cut(s) 586
EcoT14I CCWWGG 1 cut(s) 865
EgeI GGCGCC 1 cut(s) 381
EheI GGCGCC 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 865
Esp3I CGTCTC 1 cut(s) 275
FaeI CATG 1 cut(s) 668
FaiI YATR 6 cut(s) 62, 83, 435, 531, 596, 666
FatI CATG 1 cut(s) 664
Fnu4HI GCNGC 5 cut(s) 378, 396, 399, 839, 855
FokI GGATG 1 cut(s) 826
Fsp4HI GCNGC 5 cut(s) 378, 396, 399, 839, 855
FspBI CTAG 1 cut(s) 429
GlaI GCGC 1 cut(s) 381
GluI GCNGC 5 cut(s) 378, 396, 399, 839, 855
HaeII RGCGCY 1 cut(s) 383
HapII CCGG 5 cut(s) 10, 88, 234, 383, 727
HhaI GCGC 1 cut(s) 382
Hin1I GRCGYC 1 cut(s) 380
Hin1II CATG 1 cut(s) 668
Hin6I GCGC 1 cut(s) 380
HinP1I GCGC 1 cut(s) 380
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HinfI GANTC 5 cut(s) 116, 340, 356, 565, 615
HpaII CCGG 5 cut(s) 10, 88, 234, 383, 727
HphI GGTGA 3 cut(s) 129, 172, 790
Hpy166II GTNNAC 3 cut(s) 489, 543, 759
Hpy188I TCNGA 4 cut(s) 78, 99, 450, 601
Hpy188III TCNNGA 7 cut(s) 180, 316, 335, 344, 569, 665, 767
Hpy8I GTNNAC 3 cut(s) 489, 543, 759
Hpy99I CGWCG 3 cut(s) 145, 181, 484
HpyAV CCTTC 3 cut(s) 61, 361, 697
HpyCH4III ACNGT 9 cut(s) 38, 95, 254, 472, 493, 605, 632, 824, 873
HpyCH4IV ACGT 7 cut(s) 171, 320, 442, 581, 722, 737, 761
HpyCH4V TGCA 3 cut(s) 104, 754, 857
HpyF10VI GCNNNNNNNGC 1 cut(s) 377
HpyF3I CTNAG 3 cut(s) 345, 372, 410
HpySE526I ACGT 7 cut(s) 171, 320, 442, 581, 722, 737, 761
Hsp92I GRCGYC 1 cut(s) 380
Hsp92II CATG 1 cut(s) 668
HspAI GCGC 1 cut(s) 380
KasI GGCGCC 1 cut(s) 379
KpnI GGTACC 1 cut(s) 94
Kzo9I GATC 8 cut(s) 73, 125, 155, 182, 236, 293, 312, 661
LmnI GCTCC 2 cut(s) 90, 425
Lsp1109I GCAGC 1 cut(s) 841
LweI GCATC 1 cut(s) 841
MaeI CTAG 1 cut(s) 429
MaeII ACGT 7 cut(s) 171, 320, 442, 581, 722, 737, 761
MaeIII GTNAC 6 cut(s) 248, 254, 316, 502, 718, 818
MalI GATC 8 cut(s) 75, 127, 157, 184, 238, 295, 314, 663
MboI GATC 8 cut(s) 73, 125, 155, 182, 236, 293, 312, 661
MboII GAAGA 6 cut(s) 160, 298, 320, 323, 361, 571
MflI RGATCY 3 cut(s) 125, 236, 293
MluCI AATT 1 cut(s) 4
Mly113I GGCGCC 1 cut(s) 380
MlyI GAGTC 3 cut(s) 334, 350, 624
MnlI CCTC 9 cut(s) 8, 24, 185, 336, 514, 544, 549, 769, 804
MroXI GAANNNNTTC 1 cut(s) 205
MseI TTAA 3 cut(s) 243, 417, 458
MspI CCGG 5 cut(s) 10, 88, 234, 383, 727
MspR9I CCNGG 2 cut(s) 11, 588
MvaI CCWGG 1 cut(s) 588
MwoI GCNNNNNNNGC 1 cut(s) 377
NarI GGCGCC 1 cut(s) 380
NciI CCSGG 1 cut(s) 11
NdeII GATC 8 cut(s) 73, 125, 155, 182, 236, 293, 312, 661
NlaIII CATG 1 cut(s) 668
NlaIV GGNNCC 3 cut(s) 92, 238, 381
NmuCI GTSAC 2 cut(s) 316, 818
PagI TCATGA 1 cut(s) 664
PcsI WCGNNNNNNNCGW 2 cut(s) 476, 791
PdmI GAANNNNTTC 1 cut(s) 205
PfeI GAWTC 2 cut(s) 116, 565
PkrI GCNGC 5 cut(s) 379, 397, 400, 840, 856
PleI GAGTC 3 cut(s) 334, 350, 623
PluTI GGCGCC 1 cut(s) 383
PmaCI CACGTG 2 cut(s) 582, 762
PmlI CACGTG 2 cut(s) 582, 762
PpsI GAGTC 3 cut(s) 334, 350, 623
Ppu21I YACGTR 3 cut(s) 582, 738, 762
PshAI GACNNNNGTC 1 cut(s) 582
PsiI TTATAA 1 cut(s) 435
Psp1406I AACGTT 1 cut(s) 171
Psp6I CCWGG 1 cut(s) 586
PspCI CACGTG 2 cut(s) 582, 762
PspGI CCWGG 1 cut(s) 586
PspN4I GGNNCC 3 cut(s) 92, 238, 381
PsrI GAACNNNNNNTAC 2 cut(s) 620, 652
PsuI RGATCY 3 cut(s) 125, 236, 293
RsaI GTAC 6 cut(s) 35, 92, 232, 544, 725, 758
RsaNI GTAC 6 cut(s) 34, 91, 231, 543, 724, 757
SaqAI TTAA 3 cut(s) 243, 417, 458
SatI GCNGC 5 cut(s) 378, 396, 399, 839, 855
Sau3AI GATC 8 cut(s) 73, 125, 155, 182, 236, 293, 312, 661
SchI GAGTC 3 cut(s) 334, 350, 624
ScrFI CCNGG 2 cut(s) 11, 588
SfaNI GCATC 1 cut(s) 841
SfoI GGCGCC 1 cut(s) 381
SmlI CTYRAG 1 cut(s) 569
SmoI CTYRAG 1 cut(s) 569
Sse9I AATT 1 cut(s) 4
SsiI CCGC 4 cut(s) 377, 395, 398, 838
SspDI GGCGCC 1 cut(s) 379
SspI AATATT 1 cut(s) 206
SspMI CTAG 1 cut(s) 429
StyD4I CCNGG 2 cut(s) 9, 586
StyI CCWWGG 1 cut(s) 865
TaaI ACNGT 9 cut(s) 38, 95, 254, 472, 493, 605, 632, 824, 873
TaiI ACGT 7 cut(s) 174, 323, 445, 584, 725, 740, 764
TaqI TCGA 3 cut(s) 65, 179, 785
TasI AATT 1 cut(s) 4
TatI WGTACW 1 cut(s) 756
TauI GCSGC 4 cut(s) 380, 398, 401, 841
TfiI GAWTC 2 cut(s) 116, 565
Tru1I TTAA 3 cut(s) 243, 417, 458
Tru9I TTAA 3 cut(s) 243, 417, 458
TscAI CASTG 1 cut(s) 677
TseFI GTSAC 2 cut(s) 316, 818
TseI GCWGC 1 cut(s) 854
Tsp45I GTSAC 2 cut(s) 316, 818
TspDTI ATGAA 3 cut(s) 17, 578, 716
TspGWI ACGGA 8 cut(s) 158, 202, 229, 306, 438, 479, 521, 560
TspRI CASTG 1 cut(s) 677
XapI RAATTY 1 cut(s) 4
XmnI GAANNNNTTC 1 cut(s) 205
XspI CTAG 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.