AT5G63350

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011674
Physical Location & Seq
Reverse (-)
25379931 .. 25381317
1387 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G63350.1

Sequence Viewer

Length: 909 bp
ATGGACGGTAGGGGAGGTTGTTGCATAGCCAGATATGGTGGTTACGGTGGTCGCTACGGTCTCTCCAAAGCAGATCGAATCATGCTTCGTTTTCGTCCCATTGCTCCTAAACCGGCCAGCGACGGTGGAAGTGTCTCTCTCACCGGGAAGAGCGGCTCCACCACAACTACAAGTGGTGGAAGCTCCGATCTCTCAGGTAAATCTGGGAGAGGAAAGAGAAAGTATCAAAAAGATTGTTCCGGTGGGAACTCCCGAAGGTGCAATAAGAAGAAGAGAGATCTAAGTGGAGACACTGCTACTACCACGGCGGTTACATTGTCTCTTTTACCAGAGACGCCTGAGAAACGAGTTTTTCCAGATCTGAATGCTTTTCCGGTGGAGAAGCAGAAGCGAAACGGTCCTTTATGGCTGAGTTTCAACGGCGGTGGTGAGATTTTAACGCCGTACAAAACGGCGGAGATATCACGAAGGACGGTGGTGGTTTCGTCGTGTGTGACGGTTGAGCGTGTGACTGACGCTTGGATCGACGGTTATGGATTAGGGGAGACAAATCAGGAGAGGAAGATGAATCTAGTGGAAGACACGTGTCCTGGTTTTATATCGGACGGTGTAGGGAGAGTCACGTGGACCAATGAGGCGTATAAGAAGATGGCTAGAGAAGATATTAATATTCCGATGGAGGAAGGTGTACCGGAGGATATTAGTTACGATAATTTTCACGTGAACGTACGGTTAGTGATGAAGGAGAGGCCGATGCTAACGTACCCAGCTTTCACATGCAGAGTGAGATTACAGTACACGTGTCAAGATCGAGAAAGAGGATCCGTCACGGTGCCTTGTGACGTGTGGAGAATGGACGGCGGTGGTTTTGCGTGGAGGCTTGACGTTAAGGCAGCTTTGTGTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

302

Amino Acids

33.21

Weight (kDa)

9.19

Isoelectric Point (pI)

38.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7950 PF25821 161 - 301 5e-50 Domain of unknown function (DUF7950)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011674)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48510 AT5G50360 AT5G63350
fragaria_vesca FvH4_5g07700
malus_domestica MD06G1147800.v1.1 MD14G1162100.v1.1
prunus_persica Prupe.5G154700_v2.0.a1
pyrus_communis pycom14g13490 pycom72g00090
rosa_chinensis RchiOBHm_Chr7g0190771
rosa_laevigata RLG00000004500
rosa_multiflora Rmu_co8372469.1_g000001
rosa_roxburghii Rroxscaffold_3G00264340
rosa_rugosa Rorug06G0506700
rosa_samantha Rh7AG113400 Rh7BG115900 Rh7CG118400 Rh7DG117400
rosa_wichuraiana Rw7G009900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 832
AccBSI CCGCTC 1 cut(s) 153
AciI CCGC 5 cut(s) 153, 308, 423, 455, 861
AclWI GGATC 3 cut(s) 530, 816, 829
AcoI YGGCCR 1 cut(s) 114
AcvI CACGTG 4 cut(s) 585, 624, 721, 801
AcyI GRCGYC 1 cut(s) 335
AfaI GTAC 5 cut(s) 446, 690, 729, 764, 797
AflIII ACRYGT 5 cut(s) 582, 584, 798, 800, 843
AgsI TTSAA 1 cut(s) 418
AjiI CACGTC 1 cut(s) 844
AjnI CCWGG 1 cut(s) 589
AluBI AGCT 3 cut(s) 183, 770, 896
AluI AGCT 3 cut(s) 183, 770, 896
Alw26I GTCTC 6 cut(s) 65, 139, 282, 324, 326, 539
AlwI GGATC 3 cut(s) 530, 816, 829
AoxI GGCC 2 cut(s) 114, 749
ApeKI GCWGC 1 cut(s) 893
AseI ATTAAT 1 cut(s) 666
AspS9I GGNCC 2 cut(s) 398, 627
AsuC2I CCSGG 1 cut(s) 145
AsuHPI GGTGA 2 cut(s) 133, 440
AvaII GGWCC 2 cut(s) 398, 627
BamHI GGATCC 1 cut(s) 821
BanI GGYRCC 1 cut(s) 832
BbrPI CACGTG 4 cut(s) 585, 624, 721, 801
BbsI GAAGAC 1 cut(s) 585
BbvI GCAGC 1 cut(s) 905
BccI CCATC 2 cut(s) 643, 670
BceAI ACGGC 5 cut(s) 321, 427, 436, 468, 874
BciT130I CCWGG 1 cut(s) 591
BcnI CCSGG 1 cut(s) 145
BcoDI GTCTC 6 cut(s) 65, 139, 282, 324, 326, 539
BfaI CTAG 2 cut(s) 572, 654
BglII AGATCT 2 cut(s) 277, 358
BisI GCNGC 2 cut(s) 154, 894
BlsI GCNGC 2 cut(s) 155, 895
Bme1390I CCNGG 2 cut(s) 145, 591
Bme18I GGWCC 2 cut(s) 398, 627
BmgBI CACGTC 1 cut(s) 844
BmgT120I GGNCC 2 cut(s) 398, 627
BmiI GGNNCC 3 cut(s) 157, 823, 834
BmrFI CCNGG 2 cut(s) 145, 591
BmsI GCATC 1 cut(s) 744
BoxI GACNNNNGTC 1 cut(s) 585
BpiI GAAGAC 1 cut(s) 585
BpuMI CCSGG 1 cut(s) 145
BsaAI YACGTR 4 cut(s) 585, 624, 721, 801
BsaHI GRCGYC 1 cut(s) 335
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 303
BsaWI WCCGGW 3 cut(s) 239, 373, 691
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bse118I RCCGGY 1 cut(s) 112
Bse3DI GCAATG 1 cut(s) 99
BseBI CCWGG 1 cut(s) 591
BseDI CCNNGG 1 cut(s) 303
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 3 cut(s) 207, 330, 401
BseXI GCAGC 1 cut(s) 905
BseYI CCCAGC 1 cut(s) 766
BshFI GGCC 2 cut(s) 116, 751
BshNI GGYRCC 1 cut(s) 832
BsiSI CCGG 5 cut(s) 113, 144, 240, 374, 692
BsiWI CGTACG 1 cut(s) 727
BslFI GGGAC 1 cut(s) 81
BsmAI GTCTC 6 cut(s) 65, 139, 282, 324, 326, 539
BsmBI CGTCTC 1 cut(s) 326
BsmFI GGGAC 1 cut(s) 81
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 2 cut(s) 116, 751
Bso31I GGTCTC 1 cut(s) 65
Bsp143I GATC 7 cut(s) 73, 187, 277, 358, 522, 808, 821
BspACI CCGC 5 cut(s) 153, 308, 423, 455, 861
BspANI GGCC 2 cut(s) 116, 751
BspCNI CTCAG 3 cut(s) 206, 331, 402
BspLI GGNNCC 3 cut(s) 157, 823, 834
BspPI GGATC 3 cut(s) 530, 816, 829
BspQI GCTCTTC 1 cut(s) 143
BspT107I GGYRCC 1 cut(s) 832
BspTNI GGTCTC 1 cut(s) 65
BsrBI CCGCTC 1 cut(s) 153
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 112
BssAI RCCGGY 1 cut(s) 112
BssECI CCNNGG 1 cut(s) 303
BssMI GATC 7 cut(s) 73, 187, 277, 358, 522, 808, 821
BssNI GRCGYC 1 cut(s) 335
Bst2UI CCWGG 1 cut(s) 591
Bst6I CTCTTC 2 cut(s) 143, 266
BstACI GRCGYC 1 cut(s) 335
BstBAI YACGTR 4 cut(s) 585, 624, 721, 801
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 4 cut(s) 193, 281, 339, 410
BstDSI CCRYGG 1 cut(s) 303
BstKTI GATC 7 cut(s) 76, 190, 280, 361, 525, 811, 824
BstMAI GTCTC 6 cut(s) 65, 139, 282, 324, 326, 539
BstMBI GATC 7 cut(s) 73, 187, 277, 358, 522, 808, 821
BstNI CCWGG 1 cut(s) 591
BstNSI RCATGY 1 cut(s) 780
BstPAI GACNNNNGTC 1 cut(s) 585
BstSCI CCNGG 2 cut(s) 143, 589
BstV1I GCAGC 1 cut(s) 905
BstV2I GAAGAC 1 cut(s) 585
BstX2I RGATCY 3 cut(s) 277, 358, 821
BstYI RGATCY 3 cut(s) 277, 358, 821
BsuRI GGCC 2 cut(s) 116, 751
BtgI CCRYGG 1 cut(s) 303
BtrI CACGTC 1 cut(s) 844
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 1 cut(s) 291
Cac8I GCNNGC 1 cut(s) 118
Cfr10I RCCGGY 1 cut(s) 112
Cfr13I GGNCC 2 cut(s) 398, 627
CseI GACGC 2 cut(s) 343, 524
Csp6I GTAC 5 cut(s) 445, 689, 728, 763, 796
CspCI CAANNNNNGTGG 2 cut(s) 406, 441
CviAII CATG 2 cut(s) 82, 777
CviQI GTAC 5 cut(s) 445, 689, 728, 763, 796
DdeI CTNAG 4 cut(s) 193, 281, 339, 410
DpnI GATC 7 cut(s) 75, 189, 279, 360, 524, 810, 823
DpnII GATC 7 cut(s) 73, 187, 277, 358, 522, 808, 821
EaeI YGGCCR 1 cut(s) 114
Eam1104I CTCTTC 2 cut(s) 143, 266
EarI CTCTTC 2 cut(s) 143, 266
EciI GGCGGA 1 cut(s) 470
Eco31I GGTCTC 1 cut(s) 65
Eco32I GATATC 1 cut(s) 462
Eco47I GGWCC 2 cut(s) 398, 627
Eco72I CACGTG 4 cut(s) 585, 624, 721, 801
EcoRII CCWGG 1 cut(s) 589
EcoRV GATATC 1 cut(s) 462
Esp3I CGTCTC 1 cut(s) 326
FaeI CATG 2 cut(s) 85, 780
FaiI YATR 8 cut(s) 26, 36, 83, 406, 534, 599, 642, 778
FaqI GGGAC 1 cut(s) 81
FatI CATG 2 cut(s) 81, 776
Fnu4HI GCNGC 2 cut(s) 154, 894
Fsp4HI GCNGC 2 cut(s) 154, 894
FspBI CTAG 2 cut(s) 572, 654
GluI GCNGC 2 cut(s) 154, 894
GsaI CCCAGC 1 cut(s) 770
HaeIII GGCC 2 cut(s) 116, 751
HapII CCGG 5 cut(s) 113, 144, 240, 374, 692
HgaI GACGC 2 cut(s) 343, 524
Hin1I GRCGYC 1 cut(s) 335
Hin1II CATG 2 cut(s) 85, 780
HinfI GANTC 3 cut(s) 78, 568, 618
HpaII CCGG 5 cut(s) 113, 144, 240, 374, 692
HphI GGTGA 2 cut(s) 133, 440
Hpy166II GTNNAC 4 cut(s) 627, 689, 724, 798
Hpy188I TCNGA 4 cut(s) 187, 363, 604, 675
Hpy188III TCNNGA 6 cut(s) 252, 356, 465, 554, 806, 812
Hpy8I GTNNAC 4 cut(s) 627, 689, 724, 798
Hpy99I CGWCG 3 cut(s) 125, 490, 530
HpyAV CCTTC 4 cut(s) 249, 462, 677, 736
HpyCH4IV ACGT 8 cut(s) 584, 623, 720, 726, 761, 800, 843, 885
HpyCH4V TGCA 3 cut(s) 24, 261, 780
HpyF3I CTNAG 4 cut(s) 193, 281, 339, 410
HpySE526I ACGT 8 cut(s) 584, 623, 720, 726, 761, 800, 843, 885
Hsp92I GRCGYC 1 cut(s) 335
Hsp92II CATG 2 cut(s) 85, 780
Kzo9I GATC 7 cut(s) 73, 187, 277, 358, 522, 808, 821
LguI GCTCTTC 1 cut(s) 143
LmnI GCTCC 3 cut(s) 109, 161, 188
Lsp1109I GCAGC 1 cut(s) 905
LweI GCATC 1 cut(s) 744
MaeI CTAG 2 cut(s) 572, 654
MaeII ACGT 8 cut(s) 584, 623, 720, 726, 761, 800, 843, 885
MaeIII GTNAC 8 cut(s) 41, 310, 493, 508, 619, 704, 826, 839
MalI GATC 7 cut(s) 75, 189, 279, 360, 524, 810, 823
MbiI CCGCTC 1 cut(s) 153
MboI GATC 7 cut(s) 73, 187, 277, 358, 522, 808, 821
MboII GAAGA 7 cut(s) 160, 280, 283, 574, 590, 658, 671
MflI RGATCY 3 cut(s) 277, 358, 821
MluCI AATT 1 cut(s) 712
MlyI GAGTC 1 cut(s) 627
MnlI CCTC 9 cut(s) 8, 203, 552, 628, 673, 688, 741, 812, 870
MseI TTAA 3 cut(s) 437, 666, 888
MspI CCGG 5 cut(s) 113, 144, 240, 374, 692
MspR9I CCNGG 2 cut(s) 145, 591
Mva1269I GAATGC 1 cut(s) 370
MvaI CCWGG 1 cut(s) 591
NciI CCSGG 1 cut(s) 145
NdeII GATC 7 cut(s) 73, 187, 277, 358, 522, 808, 821
NlaIII CATG 2 cut(s) 85, 780
NlaIV GGNNCC 3 cut(s) 157, 823, 834
NmuCI GTSAC 5 cut(s) 493, 508, 619, 826, 839
NspI RCATGY 1 cut(s) 780
PciSI GCTCTTC 1 cut(s) 143
PcsI WCGNNNNNNNCGW 1 cut(s) 522
PctI GAATGC 1 cut(s) 370
PfeI GAWTC 2 cut(s) 78, 568
Pfl23II CGTACG 1 cut(s) 727
PkrI GCNGC 2 cut(s) 155, 895
PleI GAGTC 1 cut(s) 626
PmaCI CACGTG 4 cut(s) 585, 624, 721, 801
PmlI CACGTG 4 cut(s) 585, 624, 721, 801
PpsI GAGTC 1 cut(s) 626
Ppu21I YACGTR 4 cut(s) 585, 624, 721, 801
PshAI GACNNNNGTC 1 cut(s) 585
PshBI ATTAAT 1 cut(s) 666
Psp6I CCWGG 1 cut(s) 589
PspCI CACGTG 4 cut(s) 585, 624, 721, 801
PspFI CCCAGC 1 cut(s) 766
PspGI CCWGG 1 cut(s) 589
PspLI CGTACG 1 cut(s) 727
PspN4I GGNNCC 3 cut(s) 157, 823, 834
PspPI GGNCC 2 cut(s) 398, 627
PsuI RGATCY 3 cut(s) 277, 358, 821
RsaI GTAC 5 cut(s) 446, 690, 729, 764, 797
RsaNI GTAC 5 cut(s) 445, 689, 728, 763, 796
SapI GCTCTTC 1 cut(s) 143
SaqAI TTAA 3 cut(s) 437, 666, 888
SatI GCNGC 2 cut(s) 154, 894
Sau3AI GATC 7 cut(s) 73, 187, 277, 358, 522, 808, 821
Sau96I GGNCC 2 cut(s) 398, 627
SchI GAGTC 1 cut(s) 627
ScrFI CCNGG 2 cut(s) 145, 591
SfaNI GCATC 1 cut(s) 744
SinI GGWCC 2 cut(s) 398, 627
Sse9I AATT 1 cut(s) 712
SsiI CCGC 5 cut(s) 153, 308, 423, 455, 861
SspI AATATT 1 cut(s) 670
SspMI CTAG 2 cut(s) 572, 654
StyD4I CCNGG 2 cut(s) 143, 589
TaiI ACGT 8 cut(s) 587, 626, 723, 729, 764, 803, 846, 888
TaqI TCGA 3 cut(s) 76, 525, 811
TasI AATT 1 cut(s) 712
TatI WGTACW 1 cut(s) 795
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 2 cut(s) 78, 568
Tru1I TTAA 3 cut(s) 437, 666, 888
Tru9I TTAA 3 cut(s) 437, 666, 888
TscAI CASTG 1 cut(s) 298
TseFI GTSAC 5 cut(s) 493, 508, 619, 826, 839
TseI GCWGC 1 cut(s) 893
Tsp45I GTSAC 5 cut(s) 493, 508, 619, 826, 839
TspDTI ATGAA 2 cut(s) 581, 755
TspGWI ACGGA 1 cut(s) 814
TspRI CASTG 1 cut(s) 298
VpaK11BI GGWCC 2 cut(s) 398, 627
VspI ATTAAT 1 cut(s) 666
XceI RCATGY 1 cut(s) 780
XspI CTAG 2 cut(s) 572, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.