MD14G1162100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011674
Physical Location & Seq
Reverse (-)
25655961 .. 25657871
1911 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1162100.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGGACGGGAGAGGCGGCTGTTGCATAGCGAGGTACGGATATGGTGGCGGAGAAGAATATGGCATATCGAAGGTGGACAGGATAATGTTAAAGTTCAGACCGATCGCGCCGAAGCCGGTCGACGGAGGAGGATCAACACCGGATAAGGGTGACTTGTACGTTAAGAGTGGGAGAGGGAGAGGGAAGAGATCACGGTCTGTGAAGTCAGGGCGCAAAGCGGTAGTTGACCCAAATGACAAAAATAAAAATAAGAGAAGGAGATCGTCGAAGGCGGAGGAGGAAAAGACGGTTGTGACGCTGCCTCTGTTGCCGGAGACTCCGGAACCAAAGGAGTCTTCTGAGGAGAGAGAAAGGAACTCCTACTTGAGTCACTCGAGTTACTCATCCAACACGTGGATGAGTTTCGATAATAATAATTCTACTTCTACGACTTCTCCGACAAGTCGTATAAGTAAGGTGGTGGCGGCGAAGGTGGTTGGGAAGTCGTGCGTGACGGTGGACTGCGTGACGGATACGTGGGTGGAAGGGGAGGGGCTAGGATGTACGGACGGGGAGAGGAGGATGAACCTGGAGAGGGACACGTGTCCAGGGTTTATTTCCGACGGGACGGGGAGGGTGGTGTGGACGAATGGGGCGTACCGGAGCATGGTGGGGCAAAGGGAGGGGGAGATGATGGTGTGGCTGGTGATGAAGGAGAAGGCAGCGAGGGCGGTGGGAGTGATGAGTAATTATACGGCGGCGTTCACGTGCAGGGTGACGGTGCAGTACAGTGGGTGTGGTGGTGGGATGTCGGCAGGGGATTGGAGCTCGTTGACGCTGCCGTGTGACGTTTGGAGGATGTTGGGTGGTGGGTTTGCGTGGAGGCTGGACGTGAAGGCAGCTCTTTGTTTGGGTGGTTATAGTAATTTTATCAGTGCCGCAAAAAGATGTTATAGATTATACGGTAAAATCTCTCGCCTCCTCGTGTCTCAATCTCCCATTTTCATAGTAAGTACACGCGTTAAAAGATGTAGAGGTAAAGAATCTATCGTGGTTGTCATGTGTGTTGGAGAAAAAGCTGTTGCTTTAATCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

359

Amino Acids

39.03

Weight (kDa)

9.47

Isoelectric Point (pI)

36.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7950 PF25821 161 - 296 4.2e-46 Domain of unknown function (DUF7950)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011674)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48510 AT5G50360 AT5G63350
fragaria_vesca FvH4_5g07700
malus_domestica MD06G1147800.v1.1 MD14G1162100.v1.1
prunus_persica Prupe.5G154700_v2.0.a1
pyrus_communis pycom14g13490 pycom72g00090
rosa_chinensis RchiOBHm_Chr7g0190771
rosa_laevigata RLG00000004500
rosa_multiflora Rmu_co8372469.1_g000001
rosa_roxburghii Rroxscaffold_3G00264340
rosa_rugosa Rorug06G0506700
rosa_samantha Rh7AG113400 Rh7BG115900 Rh7CG118400 Rh7DG117400
rosa_wichuraiana Rw7G009900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 393
AccI GTMKAC 1 cut(s) 120
AccII CGCG 2 cut(s) 107, 999
AccIII TCCGGA 1 cut(s) 319
AciI CCGC 8 cut(s) 15, 48, 218, 272, 464, 710, 737, 918
AclWI GGATC 1 cut(s) 139
AcvI CACGTG 3 cut(s) 393, 582, 747
AfaI GTAC 6 cut(s) 35, 158, 544, 638, 767, 994
AfiI CCNNNNNNNGG 5 cut(s) 122, 146, 393, 574, 646
AflIII ACRYGT 4 cut(s) 390, 579, 581, 997
AjiI CACGTC 1 cut(s) 871
AjnI CCWGG 2 cut(s) 567, 586
AjuI GAANNNNNNNTTGG 2 cut(s) 319, 351
AluBI AGCT 3 cut(s) 807, 881, 1058
AluI AGCT 3 cut(s) 807, 881, 1058
Alw21I GWGCWC 1 cut(s) 809
Alw26I GTCTC 2 cut(s) 308, 972
AlwI GGATC 1 cut(s) 139
Ama87I CYCGRG 1 cut(s) 373
Aor13HI TCCGGA 1 cut(s) 319
ApeKI GCWGC 4 cut(s) 298, 701, 817, 878
AspLEI GCGC 2 cut(s) 109, 213
AsuHPI GGTGA 3 cut(s) 161, 697, 766
AvaI CYCGRG 1 cut(s) 373
BaeI ACNNNNGTAYC 2 cut(s) 504, 537
BanII GRGCYC 1 cut(s) 809
BauI CACGAG 1 cut(s) 962
BbrPI CACGTG 3 cut(s) 393, 582, 747
BbsI GAAGAC 1 cut(s) 327
Bbv12I GWGCWC 1 cut(s) 809
BbvI GCAGC 4 cut(s) 285, 713, 804, 890
BccI CCATC 1 cut(s) 667
BceAI ACGGC 2 cut(s) 750, 805
BciT130I CCWGG 2 cut(s) 569, 588
BciVI GTATCC 1 cut(s) 505
BcoDI GTCTC 2 cut(s) 308, 972
BfaI CTAG 1 cut(s) 536
BfuI GTATCC 1 cut(s) 505
BisI GCNGC 8 cut(s) 16, 299, 465, 702, 738, 818, 879, 918
BlsI GCNGC 8 cut(s) 17, 300, 466, 703, 739, 819, 880, 919
Bme1390I CCNGG 2 cut(s) 569, 588
BmeT110I CYCGRG 1 cut(s) 373
BmgBI CACGTC 1 cut(s) 871
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 2 cut(s) 569, 588
BoxI GACNNNNGTC 1 cut(s) 582
BpiI GAAGAC 1 cut(s) 327
BpmI CTGGAG 1 cut(s) 590
BpuEI CTTGAG 1 cut(s) 385
BsaAI YACGTR 4 cut(s) 393, 516, 582, 747
BsaJI CCNNGG 1 cut(s) 587
BsaWI WCCGGW 3 cut(s) 139, 319, 639
BsaXI ACNNNNNCTCC 2 cut(s) 418, 448
Bsc4I CCNNNNNNNGG 5 cut(s) 122, 146, 393, 574, 646
Bse118I RCCGGY 1 cut(s) 115
BseAI TCCGGA 1 cut(s) 319
BseBI CCWGG 2 cut(s) 569, 588
BseDI CCNNGG 1 cut(s) 587
BseGI GGATG 6 cut(s) 383, 402, 545, 567, 792, 843
BseLI CCNNNNNNNGG 5 cut(s) 122, 146, 393, 574, 646
BseMII CTCAG 1 cut(s) 330
BseRI GAGGAG 5 cut(s) 141, 290, 356, 571, 950
BseXI GCAGC 4 cut(s) 285, 713, 804, 890
BsgI GTGCAG 2 cut(s) 769, 782
Bsh1236I CGCG 2 cut(s) 107, 999
Bsh1285I CGRYCG 2 cut(s) 105, 120
BsiEI CGRYCG 2 cut(s) 105, 120
BsiHKAI GWGCWC 1 cut(s) 809
BsiHKCI CYCGRG 1 cut(s) 373
BsiSI CCGG 5 cut(s) 116, 140, 311, 320, 640
BslFI GGGAC 2 cut(s) 590, 619
BslI CCNNNNNNNGG 5 cut(s) 122, 146, 393, 574, 646
BsmAI GTCTC 2 cut(s) 308, 972
BsmFI GGGAC 2 cut(s) 590, 619
BsoBI CYCGRG 1 cut(s) 373
Bsp1286I GDGCHC 1 cut(s) 809
Bsp13I TCCGGA 1 cut(s) 319
Bsp143I GATC 4 cut(s) 102, 131, 188, 260
BspACI CCGC 8 cut(s) 15, 48, 218, 272, 464, 710, 737, 918
BspCNI CTCAG 1 cut(s) 331
BspEI TCCGGA 1 cut(s) 319
BspFNI CGCG 2 cut(s) 107, 999
BspLI GGNNCC 1 cut(s) 324
BspPI GGATC 1 cut(s) 139
BsrFI RCCGGY 1 cut(s) 115
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 1 cut(s) 587
BssMI GATC 4 cut(s) 102, 131, 188, 260
BssSI CACGAG 1 cut(s) 962
Bst2BI CACGAG 1 cut(s) 962
Bst2UI CCWGG 2 cut(s) 569, 588
Bst4CI ACNGT 6 cut(s) 195, 289, 496, 760, 770, 944
Bst6I CTCTTC 1 cut(s) 179
BstBAI YACGTR 4 cut(s) 393, 516, 582, 747
BstDEI CTNAG 1 cut(s) 339
BstF5I GGATG 6 cut(s) 383, 402, 545, 567, 792, 843
BstFNI CGCG 2 cut(s) 107, 999
BstHHI GCGC 2 cut(s) 109, 213
BstKTI GATC 4 cut(s) 105, 134, 191, 263
BstMAI GTCTC 2 cut(s) 308, 972
BstMBI GATC 4 cut(s) 102, 131, 188, 260
BstMCI CGRYCG 2 cut(s) 105, 120
BstMWI GCNNNNNNNGC 3 cut(s) 21, 307, 707
BstNI CCWGG 2 cut(s) 569, 588
BstPAI GACNNNNGTC 1 cut(s) 582
BstSCI CCNGG 2 cut(s) 567, 586
BstUI CGCG 2 cut(s) 107, 999
BstV1I GCAGC 4 cut(s) 285, 713, 804, 890
BstV2I GAAGAC 1 cut(s) 327
BsuI GTATCC 1 cut(s) 505
BtrI CACGTC 1 cut(s) 871
BtsCI GGATG 6 cut(s) 383, 402, 545, 567, 792, 843
BtsIMutI CAGTG 2 cut(s) 775, 919
CfoI GCGC 2 cut(s) 109, 213
Cfr10I RCCGGY 1 cut(s) 115
CseI GACGC 2 cut(s) 304, 823
Csp6I GTAC 6 cut(s) 34, 157, 543, 637, 766, 993
CviAII CATG 2 cut(s) 646, 1039
CviJI RGCY 8 cut(s) 18, 115, 535, 682, 807, 865, 881, 1058
CviKI_1 RGCY 8 cut(s) 18, 115, 535, 682, 807, 865, 881, 1058
CviQI GTAC 6 cut(s) 34, 157, 543, 637, 766, 993
DdeI CTNAG 1 cut(s) 339
DpnI GATC 4 cut(s) 104, 133, 190, 262
DpnII GATC 4 cut(s) 102, 131, 188, 260
Eam1104I CTCTTC 1 cut(s) 179
EarI CTCTTC 1 cut(s) 179
EciI GGCGGA 2 cut(s) 63, 287
Ecl136II GAGCTC 1 cut(s) 807
Eco24I GRGCYC 1 cut(s) 809
Eco53kI GAGCTC 1 cut(s) 807
Eco72I CACGTG 3 cut(s) 393, 582, 747
Eco88I CYCGRG 1 cut(s) 373
EcoICRI GAGCTC 1 cut(s) 807
EcoRII CCWGG 2 cut(s) 567, 586
EcoT38I GRGCYC 1 cut(s) 809
FaeI CATG 2 cut(s) 649, 1042
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FaqI GGGAC 2 cut(s) 590, 619
FatI CATG 2 cut(s) 645, 1038
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 8 cut(s) 16, 299, 465, 702, 738, 818, 879, 918
FokI GGATG 6 cut(s) 370, 409, 552, 574, 799, 850
FriOI GRGCYC 1 cut(s) 809
Fsp4HI GCNGC 8 cut(s) 16, 299, 465, 702, 738, 818, 879, 918
FspBI CTAG 1 cut(s) 536
GlaI GCGC 2 cut(s) 108, 212
GluI GCNGC 8 cut(s) 16, 299, 465, 702, 738, 818, 879, 918
GsuI CTGGAG 1 cut(s) 590
HapII CCGG 5 cut(s) 116, 140, 311, 320, 640
HgaI GACGC 2 cut(s) 304, 823
HhaI GCGC 2 cut(s) 109, 213
Hin1II CATG 2 cut(s) 649, 1042
Hin6I GCGC 2 cut(s) 107, 211
HinP1I GCGC 2 cut(s) 107, 211
HincII GTYRAC 3 cut(s) 121, 226, 813
HindII GTYRAC 3 cut(s) 121, 226, 813
HinfI GANTC 4 cut(s) 316, 332, 367, 1022
HpaII CCGG 5 cut(s) 116, 140, 311, 320, 640
HphI GGTGA 3 cut(s) 161, 697, 766
Hpy166II GTNNAC 8 cut(s) 76, 121, 226, 499, 624, 744, 813, 995
Hpy188I TCNGA 4 cut(s) 98, 340, 438, 601
Hpy188III TCNNGA 1 cut(s) 320
Hpy8I GTNNAC 8 cut(s) 76, 121, 226, 499, 624, 744, 813, 995
Hpy99I CGWCG 3 cut(s) 125, 268, 605
HpyAV CCTTC 8 cut(s) 64, 249, 262, 463, 518, 685, 691, 868
HpyCH4III ACNGT 6 cut(s) 195, 289, 496, 760, 770, 944
HpyCH4IV ACGT 7 cut(s) 159, 392, 515, 581, 746, 828, 870
HpyCH4V TGCA 3 cut(s) 24, 750, 763
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 307, 707
HpyF3I CTNAG 1 cut(s) 339
HpySE526I ACGT 7 cut(s) 159, 392, 515, 581, 746, 828, 870
Hsp92II CATG 2 cut(s) 649, 1042
HspAI GCGC 2 cut(s) 107, 211
Kpn2I TCCGGA 1 cut(s) 319
Kzo9I GATC 4 cut(s) 102, 131, 188, 260
LmnI GCTCC 2 cut(s) 642, 804
Lsp1109I GCAGC 4 cut(s) 285, 713, 804, 890
MaeI CTAG 1 cut(s) 536
MaeII ACGT 7 cut(s) 159, 392, 515, 581, 746, 828, 870
MaeIII GTNAC 8 cut(s) 149, 292, 368, 377, 490, 505, 754, 824
MalI GATC 4 cut(s) 104, 133, 190, 262
MboI GATC 4 cut(s) 102, 131, 188, 260
MboII GAAGA 3 cut(s) 65, 196, 327
MhlI GDGCHC 1 cut(s) 809
MluCI AATT 3 cut(s) 415, 727, 904
MluI ACGCGT 1 cut(s) 997
MlyI GAGTC 3 cut(s) 310, 341, 376
MmeI TCCRAC 4 cut(s) 411, 461, 624, 1027
MroI TCCGGA 1 cut(s) 319
MseI TTAA 4 cut(s) 89, 162, 1002, 1067
MslI CAYNNNNRTG 1 cut(s) 395
MspI CCGG 5 cut(s) 116, 140, 311, 320, 640
MspR9I CCNGG 2 cut(s) 569, 588
MvaI CCWGG 2 cut(s) 569, 588
MvnI CGCG 2 cut(s) 107, 999
MwoI GCNNNNNNNGC 3 cut(s) 21, 307, 707
NdeII GATC 4 cut(s) 102, 131, 188, 260
NlaIII CATG 2 cut(s) 649, 1042
NlaIV GGNNCC 1 cut(s) 324
NmuCI GTSAC 7 cut(s) 149, 292, 368, 490, 505, 754, 824
PaeR7I CTCGAG 1 cut(s) 373
PcsI WCGNNNNNNNCGW 2 cut(s) 434, 632
PfeI GAWTC 1 cut(s) 1022
PflMI CCANNNNNTGG 1 cut(s) 393
PkrI GCNGC 8 cut(s) 17, 300, 466, 703, 739, 819, 880, 919
Ple19I CGATCG 1 cut(s) 105
PleI GAGTC 3 cut(s) 310, 340, 375
PmaCI CACGTG 3 cut(s) 393, 582, 747
PmlI CACGTG 3 cut(s) 393, 582, 747
PpsI GAGTC 3 cut(s) 310, 340, 375
Ppu21I YACGTR 4 cut(s) 393, 516, 582, 747
PshAI GACNNNNGTC 1 cut(s) 582
Psp124BI GAGCTC 1 cut(s) 809
Psp6I CCWGG 2 cut(s) 567, 586
PspCI CACGTG 3 cut(s) 393, 582, 747
PspGI CCWGG 2 cut(s) 567, 586
PspN4I GGNNCC 1 cut(s) 324
PspXI VCTCGAGB 1 cut(s) 373
PvuI CGATCG 1 cut(s) 105
RsaI GTAC 6 cut(s) 35, 158, 544, 638, 767, 994
RsaNI GTAC 6 cut(s) 34, 157, 543, 637, 766, 993
RseI CAYNNNNRTG 1 cut(s) 395
SacI GAGCTC 1 cut(s) 809
SalI GTCGAC 1 cut(s) 119
SaqAI TTAA 4 cut(s) 89, 162, 1002, 1067
SatI GCNGC 8 cut(s) 16, 299, 465, 702, 738, 818, 879, 918
Sau3AI GATC 4 cut(s) 102, 131, 188, 260
SchI GAGTC 3 cut(s) 310, 341, 376
ScrFI CCNGG 2 cut(s) 569, 588
SduI GDGCHC 1 cut(s) 809
Sfr274I CTCGAG 1 cut(s) 373
SlaI CTCGAG 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 395
SmlI CTYRAG 2 cut(s) 364, 373
SmoI CTYRAG 2 cut(s) 364, 373
Sse9I AATT 3 cut(s) 415, 727, 904
SsiI CCGC 8 cut(s) 15, 48, 218, 272, 464, 710, 737, 918
SspMI CTAG 1 cut(s) 536
SstI GAGCTC 1 cut(s) 809
StyD4I CCNGG 2 cut(s) 567, 586
TaaI ACNGT 6 cut(s) 195, 289, 496, 760, 770, 944
TaiI ACGT 7 cut(s) 162, 395, 518, 584, 749, 831, 873
TaqI TCGA 5 cut(s) 68, 120, 266, 374, 405
TaqII GACCGA 1 cut(s) 115
TasI AATT 3 cut(s) 415, 727, 904
TatI WGTACW 2 cut(s) 765, 992
TauI GCSGC 4 cut(s) 18, 467, 740, 920
TfiI GAWTC 1 cut(s) 1022
Tru1I TTAA 4 cut(s) 89, 162, 1002, 1067
Tru9I TTAA 4 cut(s) 89, 162, 1002, 1067
TscAI CASTG 2 cut(s) 775, 919
TseFI GTSAC 7 cut(s) 149, 292, 368, 490, 505, 754, 824
TseI GCWGC 4 cut(s) 298, 701, 817, 878
Tsp45I GTSAC 7 cut(s) 149, 292, 368, 490, 505, 754, 824
TspDTI ATGAA 3 cut(s) 578, 704, 973
TspGWI ACGGA 4 cut(s) 51, 138, 524, 560
TspRI CASTG 2 cut(s) 775, 919
Van91I CCANNNNNTGG 1 cut(s) 393
XhoI CTCGAG 1 cut(s) 373
XmiI GTMKAC 1 cut(s) 120
XspI CTAG 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.