RLG00000004500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011674
Physical Location & Seq
Forward (+)
59880841 .. 59881659
819 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004500

Sequence Viewer

Length: 819 bp
ATGGACGGGAGAGGCGGTTGTTGTATAGCGAGGTATGAGGGAGGTGGTGGCGTTTGTGATATGTCGAAGCTGGATAGAATAATGCTGAGATTCCGTCCAATCGCTCCGAAGCCGGTTGGAGGGTCAGTCTCCGGTGGATCTACTCCGGAGAATAGTGAGAACTGTAGAAGGGGAAAGAGAAGGTGTGTGAGAGACAGGCGGTGCAATAACAGAAAGCGGAAGGCTTCTTCTCCGCCTGATAAGAAGAAAGGTGCTGTCACTACTCTGTCGCTGTTGCCGGAGAGTCCGGAACCTAAGGACTCTCCGGAAGAGCGTAGCTCAGCCACGTGGCTGACCTTCGAGAGTTTTCCCGGAAATACCGGTCCGGAGTACAAGGTGGTGAACGTGCCGGCTTTGCGGCCGGTGAGAGTGGTCGAGTCGTACGTGACGGTGGACTGCGTCACCGATACGTGGGTGGAAGGAGAACTGGGGTTTACCGACGAGGAGAGGAGGATGAGCCTGGAGACGGACACGTGTCCGGGGTTCTTATCGGACGGTTATTGTAGGGTTGTTTGGACGAACCGGGCGTACAGAAGCATGGTGGGTCAGGAGAACGAGACGGATGAGGTGTCGGTGTGTTTGGTGGTGAAGGAGACGGCGTCTGTGACGGCGGTGGCCTTGGCTCACCCGGCGTTTACGTGCAGAGTGAGAGTGCAGTACACGTGTAGATCCATCACGCTGCCTTGTGACGTGTGGAGAATGGAGGGGGGCGGGTTTGCGTGGCGGTTGGACGTGAAAGCTGCGCTGTGTCTGGGCCGGTATAGCACCAAAGCCGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

273

Amino Acids

30.04

Weight (kDa)

8.74

Isoelectric Point (pI)

48.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7950 PF25821 138 - 263 1.4e-47 Domain of unknown function (DUF7950)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011674)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48510 AT5G50360 AT5G63350
fragaria_vesca FvH4_5g07700
malus_domestica MD06G1147800.v1.1 MD14G1162100.v1.1
prunus_persica Prupe.5G154700_v2.0.a1
pyrus_communis pycom14g13490 pycom72g00090
rosa_chinensis RchiOBHm_Chr7g0190771
rosa_laevigata RLG00000004500
rosa_multiflora Rmu_co8372469.1_g000001
rosa_roxburghii Rroxscaffold_3G00264340
rosa_rugosa Rorug06G0506700
rosa_samantha Rh7AG113400 Rh7BG115900 Rh7CG118400 Rh7DG117400
rosa_wichuraiana Rw7G009900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 4 cut(s) 145, 286, 304, 364
AciI CCGC 8 cut(s) 15, 199, 217, 233, 397, 650, 750, 763
AclWI GGATC 2 cut(s) 145, 702
AcoI YGGCCR 1 cut(s) 398
AcvI CACGTG 3 cut(s) 327, 513, 702
AcyI GRCGYC 1 cut(s) 638
AfaI GTAC 4 cut(s) 371, 422, 569, 698
AfiI CCNNNNNNNGG 3 cut(s) 119, 450, 505
AflIII ACRYGT 5 cut(s) 510, 512, 699, 701, 729
AgeI ACCGGT 1 cut(s) 359
AjiI CACGTC 2 cut(s) 730, 772
AjnI CCWGG 1 cut(s) 498
AluBI AGCT 3 cut(s) 70, 318, 779
AluI AGCT 3 cut(s) 70, 318, 779
Alw26I GTCTC 5 cut(s) 133, 186, 497, 590, 626
AlwI GGATC 2 cut(s) 145, 702
Aor13HI TCCGGA 4 cut(s) 145, 286, 304, 364
AoxI GGCC 3 cut(s) 398, 654, 793
ApeKI GCWGC 2 cut(s) 718, 779
AsiGI ACCGGT 1 cut(s) 359
AspLEI GCGC 1 cut(s) 784
AspS9I GGNCC 2 cut(s) 362, 793
AsuC2I CCSGG 4 cut(s) 351, 519, 563, 668
AsuHPI GGTGA 5 cut(s) 391, 415, 433, 637, 656
AvaII GGWCC 1 cut(s) 362
AxyI CCTNAGG 1 cut(s) 294
BaeI ACNNNNGTAYC 2 cut(s) 438, 471
BbrPI CACGTG 3 cut(s) 327, 513, 702
BbvI GCAGC 2 cut(s) 705, 766
BccI CCATC 1 cut(s) 719
BceAI ACGGC 3 cut(s) 651, 663, 797
BciT130I CCWGG 1 cut(s) 500
BcnI CCSGG 4 cut(s) 351, 519, 563, 668
BcoDI GTCTC 5 cut(s) 133, 186, 497, 590, 626
BfmI CTRYAG 1 cut(s) 163
BisI GCNGC 3 cut(s) 398, 719, 780
BlpI GCTNAGC 1 cut(s) 319
BlsI GCNGC 3 cut(s) 399, 720, 781
Bme1390I CCNGG 5 cut(s) 351, 500, 519, 563, 668
Bme18I GGWCC 1 cut(s) 362
BmgBI CACGTC 2 cut(s) 730, 772
BmgT120I GGNCC 2 cut(s) 362, 793
BmiI GGNNCC 1 cut(s) 291
BmrFI CCNGG 5 cut(s) 351, 500, 519, 563, 668
BmrI ACTGGG 1 cut(s) 476
BmuI ACTGGG 1 cut(s) 476
BoxI GACNNNNGTC 1 cut(s) 513
BplI GAGNNNNNCTC 2 cut(s) 302, 334
BpmI CTGGAG 1 cut(s) 521
Bpu1102I GCTNAGC 1 cut(s) 319
BpuMI CCSGG 4 cut(s) 351, 519, 563, 668
BsaAI YACGTR 6 cut(s) 327, 424, 450, 513, 678, 702
BsaHI GRCGYC 1 cut(s) 638
BsaJI CCNNGG 2 cut(s) 518, 657
BsaWI WCCGGW 6 cut(s) 131, 145, 286, 304, 359, 364
BsaXI ACNNNNNCTCC 5 cut(s) 31, 111, 141, 623, 653
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 450, 505
Bse118I RCCGGY 5 cut(s) 112, 359, 388, 400, 795
Bse1I ACTGG 1 cut(s) 471
Bse21I CCTNAGG 1 cut(s) 294
BseAI TCCGGA 4 cut(s) 145, 286, 304, 364
BseBI CCWGG 1 cut(s) 500
BseDI CCNNGG 2 cut(s) 518, 657
BseGI GGATG 2 cut(s) 498, 607
BseLI CCNNNNNNNGG 3 cut(s) 119, 450, 505
BseMII CTCAG 2 cut(s) 77, 333
BseNI ACTGG 1 cut(s) 471
BseRI GAGGAG 2 cut(s) 497, 502
BseX3I CGGCCG 1 cut(s) 398
BseXI GCAGC 2 cut(s) 705, 766
BsgI GTGCAG 2 cut(s) 700, 713
Bsh1285I CGRYCG 1 cut(s) 401
BshFI GGCC 3 cut(s) 400, 656, 795
BshTI ACCGGT 1 cut(s) 359
BsiEI CGRYCG 1 cut(s) 401
BsiWI CGTACG 1 cut(s) 420
BslI CCNNNNNNNGG 3 cut(s) 119, 450, 505
BsmAI GTCTC 5 cut(s) 133, 186, 497, 590, 626
BsmBI CGTCTC 3 cut(s) 497, 590, 626
BsnI GGCC 3 cut(s) 400, 656, 795
Bsp13I TCCGGA 4 cut(s) 145, 286, 304, 364
Bsp143I GATC 2 cut(s) 137, 707
Bsp1720I GCTNAGC 1 cut(s) 319
BspACI CCGC 8 cut(s) 15, 199, 217, 233, 397, 650, 750, 763
BspANI GGCC 3 cut(s) 400, 656, 795
BspCNI CTCAG 2 cut(s) 78, 332
BspEI TCCGGA 4 cut(s) 145, 286, 304, 364
BspLI GGNNCC 1 cut(s) 291
BspPI GGATC 2 cut(s) 145, 702
BspQI GCTCTTC 1 cut(s) 303
BsrFI RCCGGY 5 cut(s) 112, 359, 388, 400, 795
BsrI ACTGG 1 cut(s) 471
BssAI RCCGGY 5 cut(s) 112, 359, 388, 400, 795
BssECI CCNNGG 2 cut(s) 518, 657
BssMI GATC 2 cut(s) 137, 707
BssNI GRCGYC 1 cut(s) 638
BssT1I CCWWGG 1 cut(s) 657
Bst2UI CCWGG 1 cut(s) 500
Bst4CI ACNGT 3 cut(s) 164, 430, 536
Bst6I CTCTTC 1 cut(s) 303
BstACI GRCGYC 1 cut(s) 638
BstBAI YACGTR 6 cut(s) 327, 424, 450, 513, 678, 702
BstC8I GCNNGC 1 cut(s) 390
BstDEI CTNAG 3 cut(s) 86, 294, 319
BstF5I GGATG 2 cut(s) 498, 607
BstHHI GCGC 1 cut(s) 784
BstKTI GATC 2 cut(s) 140, 710
BstMAI GTCTC 5 cut(s) 133, 186, 497, 590, 626
BstMBI GATC 2 cut(s) 137, 707
BstMCI CGRYCG 1 cut(s) 401
BstMWI GCNNNNNNNGC 3 cut(s) 394, 668, 801
BstNI CCWGG 1 cut(s) 500
BstPAI GACNNNNGTC 1 cut(s) 513
BstSCI CCNGG 5 cut(s) 349, 498, 517, 561, 666
BstSFI CTRYAG 1 cut(s) 163
BstV1I GCAGC 2 cut(s) 705, 766
BstX2I RGATCY 2 cut(s) 137, 707
BstYI RGATCY 2 cut(s) 137, 707
BstZI CGGCCG 1 cut(s) 398
Bsu36I CCTNAGG 1 cut(s) 294
BsuRI GGCC 3 cut(s) 400, 656, 795
BtrI CACGTC 2 cut(s) 730, 772
BtsCI GGATG 2 cut(s) 498, 607
Cac8I GCNNGC 1 cut(s) 390
CfoI GCGC 1 cut(s) 784
Cfr10I RCCGGY 5 cut(s) 112, 359, 388, 400, 795
Cfr13I GGNCC 2 cut(s) 362, 793
CpoI CGGWCCG 1 cut(s) 362
CseI GACGC 2 cut(s) 427, 627
Csp6I GTAC 4 cut(s) 370, 421, 568, 697
CspAI ACCGGT 1 cut(s) 359
CspI CGGWCCG 1 cut(s) 362
CviAII CATG 1 cut(s) 577
CviQI GTAC 4 cut(s) 370, 421, 568, 697
DdeI CTNAG 3 cut(s) 86, 294, 319
DpnI GATC 2 cut(s) 139, 709
DpnII GATC 2 cut(s) 137, 707
EaeI YGGCCR 1 cut(s) 398
EagI CGGCCG 1 cut(s) 398
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
EciI GGCGGA 1 cut(s) 222
EclXI CGGCCG 1 cut(s) 398
Eco130I CCWWGG 1 cut(s) 657
Eco47I GGWCC 1 cut(s) 362
Eco52I CGGCCG 1 cut(s) 398
Eco72I CACGTG 3 cut(s) 327, 513, 702
Eco81I CCTNAGG 1 cut(s) 294
EcoRII CCWGG 1 cut(s) 498
EcoT14I CCWWGG 1 cut(s) 657
ErhI CCWWGG 1 cut(s) 657
Esp3I CGTCTC 3 cut(s) 497, 590, 626
FaeI CATG 1 cut(s) 580
FaiI YATR 6 cut(s) 26, 36, 62, 578, 801, 817
FatI CATG 1 cut(s) 576
FauI CCCGC 1 cut(s) 743
Fnu4HI GCNGC 3 cut(s) 398, 719, 780
FokI GGATG 2 cut(s) 505, 614
Fsp4HI GCNGC 3 cut(s) 398, 719, 780
GlaI GCGC 1 cut(s) 783
GluI GCNGC 3 cut(s) 398, 719, 780
GsuI CTGGAG 1 cut(s) 521
HaeIII GGCC 3 cut(s) 400, 656, 795
HgaI GACGC 2 cut(s) 427, 627
HhaI GCGC 1 cut(s) 784
Hin1I GRCGYC 1 cut(s) 638
Hin1II CATG 1 cut(s) 580
Hin6I GCGC 1 cut(s) 782
HinP1I GCGC 1 cut(s) 782
HinfI GANTC 4 cut(s) 90, 283, 299, 416
HphI GGTGA 5 cut(s) 391, 415, 433, 637, 656
Hpy166II GTNNAC 5 cut(s) 382, 433, 474, 675, 699
Hpy188I TCNGA 2 cut(s) 108, 532
Hpy188III TCNNGA 6 cut(s) 146, 287, 305, 340, 365, 587
Hpy8I GTNNAC 5 cut(s) 382, 433, 474, 675, 699
Hpy99I CGWCG 1 cut(s) 482
HpyAV CCTTC 6 cut(s) 162, 174, 214, 346, 452, 622
HpyCH4III ACNGT 3 cut(s) 164, 430, 536
HpyCH4IV ACGT 9 cut(s) 326, 384, 423, 449, 512, 677, 701, 729, 771
HpyCH4V TGCA 3 cut(s) 204, 681, 694
HpyF10VI GCNNNNNNNGC 3 cut(s) 394, 668, 801
HpyF3I CTNAG 3 cut(s) 86, 294, 319
HpySE526I ACGT 9 cut(s) 326, 384, 423, 449, 512, 677, 701, 729, 771
Hsp92I GRCGYC 1 cut(s) 638
Hsp92II CATG 1 cut(s) 580
HspAI GCGC 1 cut(s) 782
Kpn2I TCCGGA 4 cut(s) 145, 286, 304, 364
KroI GCCGGC 1 cut(s) 388
KroNI GCCGGC 1 cut(s) 390
Kzo9I GATC 2 cut(s) 137, 707
LguI GCTCTTC 1 cut(s) 303
LmnI GCTCC 1 cut(s) 109
Lsp1109I GCAGC 2 cut(s) 705, 766
MaeII ACGT 9 cut(s) 326, 384, 423, 449, 512, 677, 701, 729, 771
MaeIII GTNAC 5 cut(s) 256, 424, 439, 643, 725
MalI GATC 2 cut(s) 139, 709
MboI GATC 2 cut(s) 137, 707
MboII GAAGA 3 cut(s) 219, 256, 320
MflI RGATCY 2 cut(s) 137, 707
MlyI GAGTC 3 cut(s) 292, 293, 425
MmeI TCCRAC 2 cut(s) 97, 747
MroI TCCGGA 4 cut(s) 145, 286, 304, 364
MroNI GCCGGC 1 cut(s) 388
MspR9I CCNGG 5 cut(s) 351, 500, 519, 563, 668
MvaI CCWGG 1 cut(s) 500
MwoI GCNNNNNNNGC 3 cut(s) 394, 668, 801
NaeI GCCGGC 1 cut(s) 390
NciI CCSGG 4 cut(s) 351, 519, 563, 668
NdeII GATC 2 cut(s) 137, 707
NgoMIV GCCGGC 1 cut(s) 388
NlaIII CATG 1 cut(s) 580
NlaIV GGNNCC 1 cut(s) 291
NmuCI GTSAC 5 cut(s) 256, 424, 439, 643, 725
PciSI GCTCTTC 1 cut(s) 303
PcsI WCGNNNNNNNCGW 2 cut(s) 420, 563
PdiI GCCGGC 1 cut(s) 390
PfeI GAWTC 1 cut(s) 90
Pfl23II CGTACG 1 cut(s) 420
PflFI GACNNNGTC 2 cut(s) 437, 637
PfoI TCCNGGA 1 cut(s) 349
PinAI ACCGGT 1 cut(s) 359
PkrI GCNGC 3 cut(s) 399, 720, 781
PleI GAGTC 3 cut(s) 291, 293, 424
PmaCI CACGTG 3 cut(s) 327, 513, 702
PmlI CACGTG 3 cut(s) 327, 513, 702
PpsI GAGTC 3 cut(s) 291, 293, 424
Ppu21I YACGTR 6 cut(s) 327, 424, 450, 513, 678, 702
PshAI GACNNNNGTC 1 cut(s) 513
Psp6I CCWGG 1 cut(s) 498
PspCI CACGTG 3 cut(s) 327, 513, 702
PspGI CCWGG 1 cut(s) 498
PspLI CGTACG 1 cut(s) 420
PspN4I GGNNCC 1 cut(s) 291
PspPI GGNCC 2 cut(s) 362, 793
PsrI GAACNNNNNNTAC 2 cut(s) 551, 583
PsuI RGATCY 2 cut(s) 137, 707
PsyI GACNNNGTC 2 cut(s) 437, 637
RsaI GTAC 4 cut(s) 371, 422, 569, 698
RsaNI GTAC 4 cut(s) 370, 421, 568, 697
Rsr2I CGGWCCG 1 cut(s) 362
RsrII CGGWCCG 1 cut(s) 362
SapI GCTCTTC 1 cut(s) 303
SatI GCNGC 3 cut(s) 398, 719, 780
Sau3AI GATC 2 cut(s) 137, 707
Sau96I GGNCC 2 cut(s) 362, 793
SchI GAGTC 3 cut(s) 292, 293, 425
ScrFI CCNGG 5 cut(s) 351, 500, 519, 563, 668
SfcI CTRYAG 1 cut(s) 163
SinI GGWCC 1 cut(s) 362
SsiI CCGC 8 cut(s) 15, 199, 217, 233, 397, 650, 750, 763
StyD4I CCNGG 5 cut(s) 349, 498, 517, 561, 666
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 3 cut(s) 164, 430, 536
TaiI ACGT 9 cut(s) 329, 387, 426, 452, 515, 680, 704, 732, 774
TaqI TCGA 3 cut(s) 65, 339, 414
TatI WGTACW 2 cut(s) 369, 696
TauI GCSGC 1 cut(s) 400
TfiI GAWTC 1 cut(s) 90
TseFI GTSAC 5 cut(s) 256, 424, 439, 643, 725
TseI GCWGC 2 cut(s) 718, 779
Tsp45I GTSAC 5 cut(s) 256, 424, 439, 643, 725
TspGWI ACGGA 3 cut(s) 83, 521, 614
Tth111I GACNNNGTC 2 cut(s) 437, 637
VpaK11BI GGWCC 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.