MD06G1147800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011674
Physical Location & Seq
Reverse (-)
28988378 .. 28989301
924 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1147800.v1.1.491

Sequence Viewer

Length: 924 bp
ATGGACGGGAGAGGAGGCTGTTGCATAGCGAATTACGGATATGGTGGCGGAGGAGAATATGACATGTCGAAGGTGGACAGGATCATGTTAAAGTTCAGACCGATCGCGCCGAGGCCGGTCAATGGTGGAGGATCAACTGCGGAGAAGGGTGACGTGTACGTTAAGAGTGGGAGGGGGAGAGGGAAGAGATCTCGGTGTGTGAAGTCAAAACGCAGAGCGGTAGTTTACCCTAATAACAAAAATAATAATAAGAGAAAGAGACCGTCGAAGGCGAAGGAGGAAAAGATGGTTGTGACCTTGCCTCTGTTGCCGGAGACTCCGGAGCCAAGGGAGTCTTCTGTGGAGAGAGAAAGGAACTCCTACTCGAGTCACTCGAGTTACTCGTCCACTACGTGGATGAGTTTCGAGAATAATAATTGTACCTCTAGGACTTCTCCGACAAGTCATAGAAGTAAGGTGGCGGCGGCGACGGCGATGGGGAAGTCGTGCATCACGGTGGACTGCGTGACGGACACGTGGGTGGAAGTGGAGGGGCTAGGGTGTACGGACGAGGAGAGGAGGATGAACCTGGAGAGGGACACGTGTCCAGGGTTAATTTCCGACTGGATGGGGAAGGTGGTGTGGACAAATGGGGCCTACCGGAGTATGGTAGGGCAAAGGGAGGGGGAGATGATGGTGTGGCTGGTGATGAAGGAGAAAGCAGCGAGGGCGGTGGGAGTGATGAGTAGTTATACGGCGGCGTTCACGTGCAGGGTGAGGGTGCAGTACAGTGGGTGTGGTGGTGGGACTTCGGCAGGGGATTGGTGCTCGTTGACGCTGCCGTGTGACGTGTGGAGGATGGAGGGTGGTGGGTTTGCGTGGAGGCTGGATGTGAAGGCTGCTCTTTGTTTGGGTGCTTATAGTGATTTTATCAGTGCCGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

308

Amino Acids

33.73

Weight (kDa)

9.26

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7950 PF25821 161 - 296 3.5e-47 Domain of unknown function (DUF7950)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011674)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48510 AT5G50360 AT5G63350
fragaria_vesca FvH4_5g07700
malus_domestica MD06G1147800.v1.1 MD14G1162100.v1.1
prunus_persica Prupe.5G154700_v2.0.a1
pyrus_communis pycom14g13490 pycom72g00090
rosa_chinensis RchiOBHm_Chr7g0190771
rosa_laevigata RLG00000004500
rosa_multiflora Rmu_co8372469.1_g000001
rosa_roxburghii Rroxscaffold_3G00264340
rosa_rugosa Rorug06G0506700
rosa_samantha Rh7AG113400 Rh7BG115900 Rh7CG118400 Rh7DG117400
rosa_wichuraiana Rw7G009900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 393
AccBSI CCGCTC 1 cut(s) 218
AccII CGCG 1 cut(s) 107
AccIII TCCGGA 1 cut(s) 319
AciI CCGC 7 cut(s) 48, 140, 218, 461, 464, 710, 737
AclWI GGATC 2 cut(s) 89, 139
AcvI CACGTG 3 cut(s) 516, 582, 747
AdeI CACNNNGTG 1 cut(s) 393
AfaI GTAC 4 cut(s) 158, 421, 544, 767
AfiI CCNNNNNNNGG 4 cut(s) 122, 393, 574, 646
AflIII ACRYGT 6 cut(s) 63, 153, 513, 579, 581, 828
AjiI CACGTC 2 cut(s) 154, 829
AjnI CCWGG 2 cut(s) 567, 586
AjuI GAANNNNNNNTTGG 2 cut(s) 319, 351
AleI CACNNNNGTG 1 cut(s) 518
Alw21I GWGCWC 1 cut(s) 809
Alw26I GTCTC 2 cut(s) 253, 308
AlwI GGATC 2 cut(s) 89, 139
Ama87I CYCGRG 2 cut(s) 364, 373
Aor13HI TCCGGA 1 cut(s) 319
AoxI GGCC 2 cut(s) 113, 633
ApeKI GCWGC 3 cut(s) 701, 817, 878
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 1 cut(s) 633
AsuHPI GGTGA 3 cut(s) 161, 697, 766
AvaI CYCGRG 2 cut(s) 364, 373
BbrPI CACGTG 3 cut(s) 516, 582, 747
BbsI GAAGAC 1 cut(s) 327
Bbv12I GWGCWC 1 cut(s) 809
BbvI GCAGC 3 cut(s) 713, 804, 865
BccI CCATC 5 cut(s) 280, 469, 601, 667, 832
BceAI ACGGC 4 cut(s) 486, 750, 805, 902
BciT130I CCWGG 2 cut(s) 569, 588
BcoDI GTCTC 2 cut(s) 253, 308
BfaI CTAG 2 cut(s) 426, 536
BglII AGATCT 1 cut(s) 188
BisI GCNGC 6 cut(s) 462, 465, 702, 738, 818, 879
BlsI GCNGC 6 cut(s) 463, 466, 703, 739, 819, 880
Bme1390I CCNGG 2 cut(s) 569, 588
BmeT110I CYCGRG 2 cut(s) 364, 373
BmgBI CACGTC 2 cut(s) 154, 829
BmgT120I GGNCC 1 cut(s) 633
BmiI GGNNCC 2 cut(s) 324, 634
BmrFI CCNGG 2 cut(s) 569, 588
BmsI GCATC 1 cut(s) 498
BoxI GACNNNNGTC 1 cut(s) 582
BpiI GAAGAC 1 cut(s) 327
BpmI CTGGAG 1 cut(s) 590
BsaAI YACGTR 4 cut(s) 393, 516, 582, 747
BsaI GGTCTC 1 cut(s) 253
BsaJI CCNNGG 3 cut(s) 110, 326, 587
BsaWI WCCGGW 2 cut(s) 319, 639
Bsc4I CCNNNNNNNGG 4 cut(s) 122, 393, 574, 646
Bse118I RCCGGY 1 cut(s) 115
Bse1I ACTGG 1 cut(s) 608
BseAI TCCGGA 1 cut(s) 319
BseBI CCWGG 2 cut(s) 569, 588
BseDI CCNNGG 3 cut(s) 110, 326, 587
BseGI GGATG 5 cut(s) 402, 567, 612, 843, 874
BseLI CCNNNNNNNGG 4 cut(s) 122, 393, 574, 646
BseNI ACTGG 1 cut(s) 608
BseRI GAGGAG 4 cut(s) 27, 66, 566, 571
BseXI GCAGC 3 cut(s) 713, 804, 865
BsgI GTGCAG 2 cut(s) 769, 782
Bsh1236I CGCG 1 cut(s) 107
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 2 cut(s) 115, 635
BsiEI CGRYCG 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 809
BsiHKCI CYCGRG 2 cut(s) 364, 373
BsiSI CCGG 4 cut(s) 116, 311, 320, 640
BslFI GGGAC 2 cut(s) 590, 799
BslI CCNNNNNNNGG 4 cut(s) 122, 393, 574, 646
BsmAI GTCTC 2 cut(s) 253, 308
BsmFI GGGAC 2 cut(s) 590, 799
BsnI GGCC 2 cut(s) 115, 635
Bso31I GGTCTC 1 cut(s) 253
BsoBI CYCGRG 2 cut(s) 364, 373
Bsp1286I GDGCHC 1 cut(s) 809
Bsp13I TCCGGA 1 cut(s) 319
Bsp143I GATC 4 cut(s) 81, 102, 131, 188
BspACI CCGC 7 cut(s) 48, 140, 218, 461, 464, 710, 737
BspANI GGCC 2 cut(s) 115, 635
BspEI TCCGGA 1 cut(s) 319
BspFNI CGCG 1 cut(s) 107
BspLI GGNNCC 2 cut(s) 324, 634
BspPI GGATC 2 cut(s) 89, 139
BspTNI GGTCTC 1 cut(s) 253
BsrBI CCGCTC 1 cut(s) 218
BsrFI RCCGGY 1 cut(s) 115
BsrI ACTGG 1 cut(s) 608
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 3 cut(s) 110, 326, 587
BssMI GATC 4 cut(s) 81, 102, 131, 188
BssT1I CCWWGG 1 cut(s) 326
Bst2UI CCWGG 2 cut(s) 569, 588
Bst4CI ACNGT 3 cut(s) 264, 496, 770
Bst6I CTCTTC 1 cut(s) 179
BstBAI YACGTR 4 cut(s) 393, 516, 582, 747
BstF5I GGATG 5 cut(s) 402, 567, 612, 843, 874
BstFNI CGCG 1 cut(s) 107
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 4 cut(s) 84, 105, 134, 191
BstMAI GTCTC 2 cut(s) 253, 308
BstMBI GATC 4 cut(s) 81, 102, 131, 188
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 3 cut(s) 307, 470, 707
BstNI CCWGG 2 cut(s) 569, 588
BstNSI RCATGY 1 cut(s) 67
BstPAI GACNNNNGTC 1 cut(s) 582
BstSCI CCNGG 2 cut(s) 567, 586
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 3 cut(s) 713, 804, 865
BstV2I GAAGAC 1 cut(s) 327
BstX2I RGATCY 1 cut(s) 188
BstYI RGATCY 1 cut(s) 188
BsuRI GGCC 2 cut(s) 115, 635
BtgZI GCGATG 1 cut(s) 488
BtrI CACGTC 2 cut(s) 154, 829
BtsCI GGATG 5 cut(s) 402, 567, 612, 843, 874
BtsIMutI CAGTG 2 cut(s) 775, 919
CfoI GCGC 1 cut(s) 109
Cfr10I RCCGGY 1 cut(s) 115
Cfr13I GGNCC 1 cut(s) 633
CseI GACGC 1 cut(s) 823
Csp6I GTAC 4 cut(s) 157, 420, 543, 766
CviAII CATG 2 cut(s) 64, 85
CviJI RGCY 8 cut(s) 18, 115, 325, 535, 635, 682, 865, 878
CviKI_1 RGCY 8 cut(s) 18, 115, 325, 535, 635, 682, 865, 878
CviQI GTAC 4 cut(s) 157, 420, 543, 766
DpnI GATC 4 cut(s) 83, 104, 133, 190
DpnII GATC 4 cut(s) 81, 102, 131, 188
DraIII CACNNNGTG 1 cut(s) 393
Eam1104I CTCTTC 1 cut(s) 179
EarI CTCTTC 1 cut(s) 179
EciI GGCGGA 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 326
Eco31I GGTCTC 1 cut(s) 253
Eco72I CACGTG 3 cut(s) 516, 582, 747
Eco88I CYCGRG 2 cut(s) 364, 373
EcoO109I RGGNCCY 1 cut(s) 633
EcoRII CCWGG 2 cut(s) 567, 586
EcoT14I CCWWGG 1 cut(s) 326
ErhI CCWWGG 1 cut(s) 326
FaeI CATG 2 cut(s) 67, 88
FalI AAGNNNNNCTT 2 cut(s) 319, 351
FaqI GGGAC 2 cut(s) 590, 799
FatI CATG 2 cut(s) 63, 84
Fnu4HI GCNGC 6 cut(s) 462, 465, 702, 738, 818, 879
FokI GGATG 5 cut(s) 409, 574, 619, 850, 881
Fsp4HI GCNGC 6 cut(s) 462, 465, 702, 738, 818, 879
FspBI CTAG 2 cut(s) 426, 536
GlaI GCGC 1 cut(s) 108
GluI GCNGC 6 cut(s) 462, 465, 702, 738, 818, 879
GsuI CTGGAG 1 cut(s) 590
HaeIII GGCC 2 cut(s) 115, 635
HapII CCGG 4 cut(s) 116, 311, 320, 640
HgaI GACGC 1 cut(s) 823
HhaI GCGC 1 cut(s) 109
Hin1II CATG 2 cut(s) 67, 88
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HincII GTYRAC 1 cut(s) 813
HindII GTYRAC 1 cut(s) 813
HinfI GANTC 3 cut(s) 316, 332, 367
HpaII CCGG 4 cut(s) 116, 311, 320, 640
HphI GGTGA 3 cut(s) 161, 697, 766
Hpy166II GTNNAC 9 cut(s) 76, 157, 226, 387, 499, 543, 624, 744, 813
Hpy188I TCNGA 3 cut(s) 98, 438, 601
Hpy188III TCNNGA 2 cut(s) 320, 406
Hpy8I GTNNAC 9 cut(s) 76, 157, 226, 387, 499, 543, 624, 744, 813
Hpy99I CGWCG 2 cut(s) 268, 472
HpyAV CCTTC 7 cut(s) 64, 139, 262, 268, 607, 685, 868
HpyCH4III ACNGT 3 cut(s) 264, 496, 770
HpyCH4IV ACGT 7 cut(s) 153, 159, 392, 515, 581, 746, 828
HpyCH4V TGCA 4 cut(s) 24, 489, 750, 763
HpyF10VI GCNNNNNNNGC 3 cut(s) 307, 470, 707
HpySE526I ACGT 7 cut(s) 153, 159, 392, 515, 581, 746, 828
Hsp92II CATG 2 cut(s) 67, 88
HspAI GCGC 1 cut(s) 107
Kpn2I TCCGGA 1 cut(s) 319
Kzo9I GATC 4 cut(s) 81, 102, 131, 188
LmnI GCTCC 1 cut(s) 322
Lsp1109I GCAGC 3 cut(s) 713, 804, 865
LweI GCATC 1 cut(s) 498
MaeI CTAG 2 cut(s) 426, 536
MaeII ACGT 7 cut(s) 153, 159, 392, 515, 581, 746, 828
MaeIII GTNAC 6 cut(s) 149, 292, 368, 377, 505, 824
MalI GATC 4 cut(s) 83, 104, 133, 190
MbiI CCGCTC 1 cut(s) 218
MboI GATC 4 cut(s) 81, 102, 131, 188
MboII GAAGA 2 cut(s) 196, 327
MflI RGATCY 1 cut(s) 188
MhlI GDGCHC 1 cut(s) 809
MluCI AATT 3 cut(s) 31, 415, 594
MlyI GAGTC 3 cut(s) 310, 341, 376
MmeI TCCRAC 2 cut(s) 461, 624
MroI TCCGGA 1 cut(s) 319
MseI TTAA 3 cut(s) 89, 162, 593
MslI CAYNNNNRTG 2 cut(s) 494, 518
MspI CCGG 4 cut(s) 116, 311, 320, 640
MspR9I CCNGG 2 cut(s) 569, 588
MvaI CCWGG 2 cut(s) 569, 588
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 3 cut(s) 307, 470, 707
NdeII GATC 4 cut(s) 81, 102, 131, 188
NlaIII CATG 2 cut(s) 67, 88
NlaIV GGNNCC 2 cut(s) 324, 634
NmeAIII GCCGAG 1 cut(s) 135
NmuCI GTSAC 5 cut(s) 149, 292, 368, 505, 824
NspI RCATGY 1 cut(s) 67
OliI CACNNNNGTG 1 cut(s) 518
PaeR7I CTCGAG 2 cut(s) 364, 373
PciI ACATGT 1 cut(s) 63
PcsI WCGNNNNNNNCGW 3 cut(s) 371, 380, 389
PflMI CCANNNNNTGG 1 cut(s) 393
PkrI GCNGC 6 cut(s) 463, 466, 703, 739, 819, 880
Ple19I CGATCG 1 cut(s) 105
PleI GAGTC 3 cut(s) 310, 340, 375
PmaCI CACGTG 3 cut(s) 516, 582, 747
PmlI CACGTG 3 cut(s) 516, 582, 747
PpsI GAGTC 3 cut(s) 310, 340, 375
Ppu21I YACGTR 4 cut(s) 393, 516, 582, 747
PscI ACATGT 1 cut(s) 63
PshAI GACNNNNGTC 1 cut(s) 582
Psp6I CCWGG 2 cut(s) 567, 586
PspCI CACGTG 3 cut(s) 516, 582, 747
PspGI CCWGG 2 cut(s) 567, 586
PspN4I GGNNCC 2 cut(s) 324, 634
PspPI GGNCC 1 cut(s) 633
PspXI VCTCGAGB 2 cut(s) 364, 373
PsuI RGATCY 1 cut(s) 188
PvuI CGATCG 1 cut(s) 105
RsaI GTAC 4 cut(s) 158, 421, 544, 767
RsaNI GTAC 4 cut(s) 157, 420, 543, 766
RseI CAYNNNNRTG 2 cut(s) 494, 518
SaqAI TTAA 3 cut(s) 89, 162, 593
SatI GCNGC 6 cut(s) 462, 465, 702, 738, 818, 879
Sau3AI GATC 4 cut(s) 81, 102, 131, 188
Sau96I GGNCC 1 cut(s) 633
SchI GAGTC 3 cut(s) 310, 341, 376
ScrFI CCNGG 2 cut(s) 569, 588
SduI GDGCHC 1 cut(s) 809
SfaNI GCATC 1 cut(s) 498
Sfr274I CTCGAG 2 cut(s) 364, 373
SlaI CTCGAG 2 cut(s) 364, 373
SmiMI CAYNNNNRTG 2 cut(s) 494, 518
SmlI CTYRAG 2 cut(s) 364, 373
SmoI CTYRAG 2 cut(s) 364, 373
Sse9I AATT 3 cut(s) 31, 415, 594
SsiI CCGC 7 cut(s) 48, 140, 218, 461, 464, 710, 737
SspMI CTAG 2 cut(s) 426, 536
StyD4I CCNGG 2 cut(s) 567, 586
StyI CCWWGG 1 cut(s) 326
TaaI ACNGT 3 cut(s) 264, 496, 770
TaiI ACGT 7 cut(s) 156, 162, 395, 518, 584, 749, 831
TaqI TCGA 5 cut(s) 68, 266, 365, 374, 405
TaqII GACCGA 1 cut(s) 115
TasI AATT 3 cut(s) 31, 415, 594
TatI WGTACW 1 cut(s) 765
TauI GCSGC 3 cut(s) 464, 467, 740
Tru1I TTAA 3 cut(s) 89, 162, 593
Tru9I TTAA 3 cut(s) 89, 162, 593
TscAI CASTG 2 cut(s) 775, 919
TseFI GTSAC 5 cut(s) 149, 292, 368, 505, 824
TseI GCWGC 3 cut(s) 701, 817, 878
Tsp45I GTSAC 5 cut(s) 149, 292, 368, 505, 824
TspDTI ATGAA 2 cut(s) 578, 704
TspGWI ACGGA 3 cut(s) 51, 524, 560
TspRI CASTG 2 cut(s) 775, 919
Van91I CCANNNNNTGG 1 cut(s) 393
XceI RCATGY 1 cut(s) 67
XhoI CTCGAG 2 cut(s) 364, 373
XspI CTAG 2 cut(s) 426, 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.