AT5G51460

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007067
Physical Location & Seq
Forward (+)
20900859 .. 20904647
3789 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G51460.5

Sequence Viewer

Length: 1158 bp
ATGGACATGAAATCTGGTCACTCGTCTCCTGTAATGACTGATTCTCCACCAATAAGCAACTCAAGATTAACCATTCGTCAGAATAGACTTCCTTACTCATCAGCAGCAGCCACGGCTATCTCACAGAACAACAATCTCTTACTAACCGTTCCAAGAAAGAAAACTGGGATCCTTGATGATGTTAAGTCTAATGGTTGGCTTGATGCGATGAAATCTTCTTCTCCTCCTCCTACAATACTTAACAAAGATAACTTAAGCAATGATGCTACGGATATGACTTATCGCGAATGGATGCAGCTCAAGTATCCATCAGCTCTTACCTCTTTTGAGAAAATCATGAGTTTTGCAAAAGGCAAAAGAATAGCATTGTTTCTTGATTATGACGGGACACTTTCGCCTATTGTTGAGGAACCTGATTGTGCATACATGTCAAGTGCTATGCGTAGTGCAGTGCAAAATGTTGCCAAGTATTTCCCTACCGCGATCATTAGTGGAAGAAGCCGGGATAAGGTGTATGAGTTTGTTAATTTGAGTGAACTTTATTACGCCGGAAGCCATGGAATGGACATCATGAGTCCCGCAGGAGAATCTTTAAACCATGAACATAGCCGTACTGTATCAGTTTACGAACAGGGGAAAGATGTAAATCTATTCCAGCCTGCTAGCGAGTTTCTCCCGATGATCGATAAGGTGCTTTGTTCTCTTATAGAGAGTACAAAAGATATCAAAGGGGTAAAAGTAGAAGACAACAAGTTCTGCATCTCTGTGCATTACCGCAATGTAGAAGAAAAGAACTGGACATTGGTTGCACAGTGTGTAGATGATGTCATCAGAACATATCCAAAACTACGGCTAACACATGGCCGGAAGGTTTTAGAGATCCGTCCTGTGATTGACTGGGACAAAGGGAAAGCTGTGACATTTCTACTTGAATCACTCGGCCTAAACAACTGTGAGGATGTTCTTCCAATCTATGTTGGGGATGATCGAACAGACGAAGATGCATTTAAGGTACTACGAGATGGACCAAACCACGGTTATGGTATATTAGTCTCTGCTGTGCCTAAAGACAGCAATGCCTTTTACTCGCTTCGTGACCCGTCTGAGGTGATGGAGTTTCTGAAGTCATTGGTGACATGGAAGAGATCAATGGGTTAA

Protein Analysis

385

Amino Acids

43.19

Weight (kDa)

6.21

Isoelectric Point (pI)

45.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 124 - 368 1.7e-75 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 562
AccII CGCG 2 cut(s) 285, 482
AciI CCGC 3 cut(s) 480, 579, 775
AclWI GGATC 3 cut(s) 163, 176, 874
AcoI YGGCCR 1 cut(s) 862
AcuI CTGAAG 1 cut(s) 1142
AdeI CACNNNGTG 1 cut(s) 815
AfaI GTAC 3 cut(s) 613, 715, 1014
AfiI CCNNNNNNNGG 4 cut(s) 508, 562, 1034, 1105
AflII CTTAAG 1 cut(s) 253
AflIII ACRYGT 1 cut(s) 426
AgsI TTSAA 1 cut(s) 932
AjuI GAANNNNNNNTTGG 2 cut(s) 785, 817
AluBI AGCT 3 cut(s) 298, 314, 914
AluI AGCT 3 cut(s) 298, 314, 914
Alw26I GTCTC 2 cut(s) 30, 1057
AlwI GGATC 3 cut(s) 163, 176, 874
AoxI GGCC 2 cut(s) 862, 940
ApeKI GCWGC 3 cut(s) 104, 107, 295
AspS9I GGNCC 1 cut(s) 1025
AsuC2I CCSGG 1 cut(s) 503
AsuHPI GGTGA 2 cut(s) 1120, 1144
AsuNHI GCTAGC 1 cut(s) 662
AvaII GGWCC 1 cut(s) 1025
BamHI GGATCC 1 cut(s) 168
BbsI GAAGAC 1 cut(s) 750
BbvI GCAGC 3 cut(s) 116, 119, 307
BccI CCATC 3 cut(s) 316, 1016, 1105
BceAI ACGGC 3 cut(s) 129, 594, 866
BciVI GTATCC 1 cut(s) 315
BcnI CCSGG 1 cut(s) 503
BcoDI GTCTC 2 cut(s) 30, 1057
BfaI CTAG 1 cut(s) 663
BfrI CTTAAG 1 cut(s) 253
BfuI GTATCC 1 cut(s) 315
BisI GCNGC 3 cut(s) 105, 108, 296
BlsI GCNGC 3 cut(s) 106, 109, 297
Bme1390I CCNGG 1 cut(s) 503
Bme18I GGWCC 1 cut(s) 1025
BmgT120I GGNCC 1 cut(s) 1025
BmiI GGNNCC 2 cut(s) 170, 411
BmrFI CCNGG 1 cut(s) 503
BmrI ACTGGG 2 cut(s) 174, 907
BmsI GCATC 5 cut(s) 193, 253, 282, 768, 991
BmtI GCTAGC 1 cut(s) 666
BmuI ACTGGG 2 cut(s) 174, 907
BpiI GAAGAC 1 cut(s) 750
BpuEI CTTGAG 2 cut(s) 46, 284
BpuMI CCSGG 1 cut(s) 503
Bsa29I ATCGAT 1 cut(s) 684
BsaBI GATNNNNATC 1 cut(s) 645
BsaJI CCNNGG 3 cut(s) 111, 556, 1033
BsaXI ACNNNNNCTCC 2 cut(s) 28, 58
Bsc4I CCNNNNNNNGG 4 cut(s) 508, 562, 1034, 1105
Bse1I ACTGG 3 cut(s) 169, 800, 902
Bse3DI GCAATG 3 cut(s) 265, 784, 1081
Bse8I GATNNNNATC 1 cut(s) 645
BseCI ATCGAT 1 cut(s) 684
BseDI CCNNGG 3 cut(s) 111, 556, 1033
BseGI GGATG 3 cut(s) 297, 964, 988
BseJI GATNNNNATC 1 cut(s) 645
BseLI CCNNNNNNNGG 4 cut(s) 508, 562, 1034, 1105
BseMI GCAATG 3 cut(s) 265, 784, 1081
BseMII CTCAG 1 cut(s) 1095
BseNI ACTGG 3 cut(s) 169, 800, 902
BseRI GAGGAG 2 cut(s) 213, 216
BseXI GCAGC 3 cut(s) 116, 119, 307
BsgI GTGCAG 1 cut(s) 468
Bsh1236I CGCG 2 cut(s) 285, 482
BshFI GGCC 2 cut(s) 864, 942
BshVI ATCGAT 1 cut(s) 684
BsiSI CCGG 3 cut(s) 502, 549, 865
BslFI GGGAC 3 cut(s) 400, 561, 914
BslI CCNNNNNNNGG 4 cut(s) 508, 562, 1034, 1105
BsmAI GTCTC 2 cut(s) 30, 1057
BsmBI CGTCTC 1 cut(s) 30
BsmFI GGGAC 3 cut(s) 400, 561, 914
BsnI GGCC 2 cut(s) 864, 942
Bsp143I GATC 6 cut(s) 168, 483, 681, 879, 985, 1145
Bsp19I CCATGG 1 cut(s) 556
Bsp68I TCGCGA 1 cut(s) 285
BspACI CCGC 3 cut(s) 480, 579, 775
BspANI GGCC 2 cut(s) 864, 942
BspCNI CTCAG 1 cut(s) 1096
BspDI ATCGAT 1 cut(s) 684
BspFNI CGCG 2 cut(s) 285, 482
BspHI TCATGA 2 cut(s) 336, 570
BspLI GGNNCC 2 cut(s) 170, 411
BspOI GCTAGC 1 cut(s) 666
BspPI GGATC 3 cut(s) 163, 176, 874
BspTI CTTAAG 1 cut(s) 253
BsrDI GCAATG 3 cut(s) 265, 784, 1081
BsrI ACTGG 3 cut(s) 169, 800, 902
BssECI CCNNGG 3 cut(s) 111, 556, 1033
BssMI GATC 6 cut(s) 168, 483, 681, 879, 985, 1145
BssT1I CCWWGG 1 cut(s) 556
Bst4CI ACNGT 5 cut(s) 148, 616, 813, 953, 1037
Bst6I CTCTTC 1 cut(s) 1136
BstAFI CTTAAG 1 cut(s) 253
BstC8I GCNNGC 2 cut(s) 660, 664
BstDEI CTNAG 1 cut(s) 1104
BstDSI CCRYGG 3 cut(s) 111, 556, 1033
BstF5I GGATG 3 cut(s) 297, 964, 988
BstFNI CGCG 2 cut(s) 285, 482
BstKTI GATC 6 cut(s) 171, 486, 684, 882, 988, 1148
BstMAI GTCTC 2 cut(s) 30, 1057
BstMBI GATC 6 cut(s) 168, 483, 681, 879, 985, 1145
BstMWI GCNNNNNNNGC 1 cut(s) 113
BstNSI RCATGY 1 cut(s) 430
BstSCI CCNGG 1 cut(s) 501
BstUI CGCG 2 cut(s) 285, 482
BstV1I GCAGC 3 cut(s) 116, 119, 307
BstV2I GAAGAC 1 cut(s) 750
BstX2I RGATCY 2 cut(s) 168, 879
BstXI CCANNNNNNTGG 1 cut(s) 1040
BstYI RGATCY 2 cut(s) 168, 879
Bsu15I ATCGAT 1 cut(s) 684
BsuI GTATCC 1 cut(s) 315
BsuRI GGCC 2 cut(s) 864, 942
BsuTUI ATCGAT 1 cut(s) 684
BtgI CCRYGG 3 cut(s) 111, 556, 1033
BtgZI GCGATG 1 cut(s) 221
BtsCI GGATG 3 cut(s) 297, 964, 988
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 2 cut(s) 456, 818
BtuMI TCGCGA 1 cut(s) 285
Cac8I GCNNGC 2 cut(s) 660, 664
CciI TCATGA 2 cut(s) 336, 570
Cfr13I GGNCC 1 cut(s) 1025
ClaI ATCGAT 1 cut(s) 684
Csp6I GTAC 3 cut(s) 612, 714, 1013
CviAII CATG 8 cut(s) 7, 337, 427, 557, 571, 599, 860, 1137
CviQI GTAC 3 cut(s) 612, 714, 1013
DdeI CTNAG 1 cut(s) 1104
DpnI GATC 6 cut(s) 170, 485, 683, 881, 987, 1147
DpnII GATC 6 cut(s) 168, 483, 681, 879, 985, 1145
DraI TTTAAA 1 cut(s) 594
DraIII CACNNNGTG 1 cut(s) 815
EaeI YGGCCR 1 cut(s) 862
Eam1104I CTCTTC 1 cut(s) 1136
EarI CTCTTC 1 cut(s) 1136
Eco130I CCWWGG 1 cut(s) 556
Eco32I GATATC 1 cut(s) 724
Eco47I GGWCC 1 cut(s) 1025
Eco57I CTGAAG 1 cut(s) 1142
EcoRV GATATC 1 cut(s) 724
EcoT14I CCWWGG 1 cut(s) 556
EcoT22I ATGCAT 1 cut(s) 1006
ErhI CCWWGG 1 cut(s) 556
Esp3I CGTCTC 1 cut(s) 30
FaeI CATG 8 cut(s) 10, 340, 430, 560, 574, 602, 863, 1140
FaqI GGGAC 3 cut(s) 400, 561, 914
FatI CATG 8 cut(s) 6, 336, 426, 556, 570, 598, 859, 1136
FauI CCCGC 1 cut(s) 586
Fnu4HI GCNGC 3 cut(s) 105, 108, 296
FokI GGATG 3 cut(s) 304, 971, 995
Fsp4HI GCNGC 3 cut(s) 105, 108, 296
FspBI CTAG 1 cut(s) 663
GluI GCNGC 3 cut(s) 105, 108, 296
HaeIII GGCC 2 cut(s) 864, 942
HapII CCGG 3 cut(s) 502, 549, 865
Hin1II CATG 8 cut(s) 10, 340, 430, 560, 574, 602, 863, 1140
HinfI GANTC 4 cut(s) 41, 574, 587, 932
HpaII CCGG 3 cut(s) 502, 549, 865
HphI GGTGA 2 cut(s) 1120, 1144
Hpy166II GTNNAC 2 cut(s) 536, 625
Hpy188I TCNGA 4 cut(s) 81, 833, 1105, 1122
Hpy188III TCNNGA 7 cut(s) 63, 284, 337, 374, 571, 676, 1094
Hpy8I GTNNAC 2 cut(s) 536, 625
HpyAV CCTTC 1 cut(s) 862
HpyCH4III ACNGT 5 cut(s) 148, 616, 813, 953, 1037
HpyCH4V TGCA 9 cut(s) 295, 347, 422, 449, 454, 759, 769, 809, 1004
HpyF10VI GCNNNNNNNGC 1 cut(s) 113
HpyF3I CTNAG 1 cut(s) 1104
Hsp92II CATG 8 cut(s) 10, 340, 430, 560, 574, 602, 863, 1140
Kzo9I GATC 6 cut(s) 168, 483, 681, 879, 985, 1145
Lsp1109I GCAGC 3 cut(s) 116, 119, 307
LweI GCATC 5 cut(s) 193, 253, 282, 768, 991
MaeI CTAG 1 cut(s) 663
MaeIII GTNAC 4 cut(s) 17, 916, 1094, 1132
MalI GATC 6 cut(s) 170, 485, 683, 881, 987, 1147
MboI GATC 6 cut(s) 168, 483, 681, 879, 985, 1145
MboII GAAGA 8 cut(s) 207, 210, 507, 755, 797, 956, 1010, 1153
MflI RGATCY 2 cut(s) 168, 879
MluCI AATT 1 cut(s) 526
MlyI GAGTC 1 cut(s) 583
MnlI CCTC 6 cut(s) 234, 237, 331, 400, 949, 1099
Mph1103I ATGCAT 1 cut(s) 1006
MseI TTAA 8 cut(s) 68, 183, 240, 254, 525, 593, 1008, 1156
MslI CAYNNNNRTG 2 cut(s) 764, 1038
MspCI CTTAAG 1 cut(s) 253
MspI CCGG 3 cut(s) 502, 549, 865
MspR9I CCNGG 1 cut(s) 503
MvnI CGCG 2 cut(s) 285, 482
MwoI GCNNNNNNNGC 1 cut(s) 113
NciI CCSGG 1 cut(s) 503
NcoI CCATGG 1 cut(s) 556
NdeII GATC 6 cut(s) 168, 483, 681, 879, 985, 1145
NheI GCTAGC 1 cut(s) 662
NlaIII CATG 8 cut(s) 10, 340, 430, 560, 574, 602, 863, 1140
NlaIV GGNNCC 2 cut(s) 170, 411
NmeAIII GCCGAG 1 cut(s) 918
NmuCI GTSAC 4 cut(s) 17, 916, 1094, 1132
NruI TCGCGA 1 cut(s) 285
NsiI ATGCAT 1 cut(s) 1006
NspI RCATGY 1 cut(s) 430
PagI TCATGA 2 cut(s) 336, 570
PciI ACATGT 1 cut(s) 426
PfeI GAWTC 3 cut(s) 41, 587, 932
PflMI CCANNNNNTGG 1 cut(s) 562
PkrI GCNGC 3 cut(s) 106, 109, 297
PleI GAGTC 1 cut(s) 582
PpsI GAGTC 1 cut(s) 582
PscI ACATGT 1 cut(s) 426
PspN4I GGNNCC 2 cut(s) 170, 411
PspPI GGNCC 1 cut(s) 1025
PsuI RGATCY 2 cut(s) 168, 879
RruI TCGCGA 1 cut(s) 285
RsaI GTAC 3 cut(s) 613, 715, 1014
RsaNI GTAC 3 cut(s) 612, 714, 1013
RseI CAYNNNNRTG 2 cut(s) 764, 1038
SaqAI TTAA 8 cut(s) 68, 183, 240, 254, 525, 593, 1008, 1156
SatI GCNGC 3 cut(s) 105, 108, 296
Sau3AI GATC 6 cut(s) 168, 483, 681, 879, 985, 1145
Sau96I GGNCC 1 cut(s) 1025
SchI GAGTC 1 cut(s) 583
ScrFI CCNGG 1 cut(s) 503
SfaNI GCATC 5 cut(s) 193, 253, 282, 768, 991
SinI GGWCC 1 cut(s) 1025
SmiMI CAYNNNNRTG 2 cut(s) 764, 1038
SmlI CTYRAG 3 cut(s) 61, 253, 299
SmoI CTYRAG 3 cut(s) 61, 253, 299
Sse9I AATT 1 cut(s) 526
SsiI CCGC 3 cut(s) 480, 579, 775
SspMI CTAG 1 cut(s) 663
StyD4I CCNGG 1 cut(s) 501
StyI CCWWGG 1 cut(s) 556
TaaI ACNGT 5 cut(s) 148, 616, 813, 953, 1037
TaqI TCGA 2 cut(s) 684, 988
TasI AATT 1 cut(s) 526
TatI WGTACW 1 cut(s) 713
TfiI GAWTC 3 cut(s) 41, 587, 932
Tru1I TTAA 8 cut(s) 68, 183, 240, 254, 525, 593, 1008, 1156
Tru9I TTAA 8 cut(s) 68, 183, 240, 254, 525, 593, 1008, 1156
TscAI CASTG 2 cut(s) 456, 818
TseFI GTSAC 4 cut(s) 17, 916, 1094, 1132
TseI GCWGC 3 cut(s) 104, 107, 295
Tsp45I GTSAC 4 cut(s) 17, 916, 1094, 1132
TspDTI ATGAA 3 cut(s) 23, 224, 615
TspGWI ACGGA 2 cut(s) 284, 872
TspRI CASTG 2 cut(s) 456, 818
Van91I CCANNNNNTGG 1 cut(s) 562
Vha464I CTTAAG 1 cut(s) 253
VpaK11BI GGWCC 1 cut(s) 1025
XceI RCATGY 1 cut(s) 430
XspI CTAG 1 cut(s) 663
Zsp2I ATGCAT 1 cut(s) 1006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.